Rh7DG228000

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009609
Physical Location & Seq
Reverse (-)
22438653 .. 22443892
5240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG228000.1

Sequence Viewer

Length: 756 bp
ATGGAGGTGGCCACCGCAGCTTCCAGCTTCGCGCTTCACACGCGCATCTCAGCCGCTCCCCGAGCCCTAAACCCTAACCGCGTCCAAACGATGTCGTCTTGCTCTCCTCTCCGAGCCTCCCTCTCCTCCAACTTCCTCTCGCCCTTCGCCGGCGGCAGCGTCACCTCCGACTTCTCCGGCCACAAGCTCCGCCCTTCCTCTCTCAATCCGGTGTCGTCTCGTGGCTCTAAGCCCAAACGCGGTGTCGTCACCATGGTTATTCCATTTCAAAGGGGAAGTGCATGGGAGCAACCACCTCCAGACCTGGCATCATATCTATACAAGAATCGAATTGTTTACTTGGGAATGTCGCTTGTTCCTTCAGTAACAGAGTTGATTCTTGCAGAATTTCTCTACCTTCAGTATGAGGACGATACAAAGCCTATCTACTTGTACATAAATTCCACTGGAACTACCAAGGGTGGTGAGAAATTGGGTTACGAGACAGAGGCTCTTGCAATATATGATGTCATGAGGTATGTCAAGCCACCAATATTTACTCTTTGTGTTGGTAATGCTTGGGGAGAAGCCGCTTTACTCTTGGCTGCTGGTGCAAAGGGAAACCGTTCTGCGTTACCCTCATCAACAATCATGATAAAGCAGCCAATTGCAAGGTTCCAAGGTCAAGCAACAGATATTGAACTTGCAAGAAAAGAAGTGAAGAATGTGAAAGATGAGTTGAGATTTTTGGAGAGAGGGAGGGAGGGAGAACCTTAG

Protein Analysis

251

Amino Acids

27.39

Weight (kDa)

9.39

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 101 - 240 1.3e-36 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 56
AccII CGCG 4 cut(s) 32, 43, 81, 240
AciI CCGC 7 cut(s) 15, 54, 79, 153, 190, 240, 570
AcoI YGGCCR 2 cut(s) 9, 178
AcsI RAATTY 2 cut(s) 386, 439
AcuI CTGAAG 2 cut(s) 345, 383
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 149, 239
AgsI TTSAA 2 cut(s) 269, 680
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 3 cut(s) 20, 27, 187
AluI AGCT 3 cut(s) 20, 27, 187
Alw26I GTCTC 2 cut(s) 222, 476
Ama87I CYCGRG 1 cut(s) 60
AoxI GGCC 2 cut(s) 9, 178
ApeKI GCWGC 4 cut(s) 17, 156, 584, 640
ApoI RAATTY 2 cut(s) 386, 439
ArsI GACNNNNNNTTYG 2 cut(s) 228, 260
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 34, 45
AsuHPI GGTGA 3 cut(s) 154, 241, 476
AvaI CYCGRG 1 cut(s) 60
BalI TGGCCA 1 cut(s) 11
BanII GRGCYC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BauI CACGAG 1 cut(s) 219
BbvI GCAGC 4 cut(s) 29, 168, 571, 652
BciT130I CCWGG 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 222, 476
BisI GCNGC 7 cut(s) 18, 54, 154, 157, 570, 585, 641
BlsI GCNGC 7 cut(s) 19, 55, 155, 158, 571, 586, 642
Bme1390I CCNGG 1 cut(s) 305
BmeT110I CYCGRG 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 656
BmrFI CCNGG 1 cut(s) 305
BmsI GCATC 2 cut(s) 54, 317
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 252, 456, 658
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 239
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 451
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 252, 456, 658
BseLI CCNNNNNNNGG 2 cut(s) 149, 239
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 2 cut(s) 96, 115
BseXI GCAGC 4 cut(s) 29, 168, 571, 652
Bsh1236I CGCG 4 cut(s) 32, 43, 81, 240
BshFI GGCC 2 cut(s) 11, 180
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 3 cut(s) 150, 177, 209
BslI CCNNNNNNNGG 2 cut(s) 149, 239
BsmAI GTCTC 2 cut(s) 222, 476
BsmBI CGTCTC 1 cut(s) 222
BsnI GGCC 2 cut(s) 11, 180
BsoBI CYCGRG 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 432
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 7 cut(s) 15, 54, 79, 153, 190, 240, 570
BspANI GGCC 2 cut(s) 11, 180
BspCNI CTCAG 1 cut(s) 62
BspFNI CGCG 4 cut(s) 32, 43, 81, 240
BspHI TCATGA 2 cut(s) 510, 630
BspLI GGNNCC 1 cut(s) 656
BsrBI CCGCTC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 252, 456, 658
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 3 cut(s) 252, 456, 658
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 605
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 3 cut(s) 49, 228, 753
BstDSI CCRYGG 1 cut(s) 252
BstFNI CGCG 4 cut(s) 32, 43, 81, 240
BstHHI GCGC 2 cut(s) 34, 45
BstMAI GTCTC 2 cut(s) 222, 476
BstMWI GCNNNNNNNGC 4 cut(s) 17, 40, 62, 590
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 303
BstUI CGCG 4 cut(s) 32, 43, 81, 240
BstV1I GCAGC 4 cut(s) 29, 168, 571, 652
BsuRI GGCC 2 cut(s) 11, 180
BtgI CCRYGG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 151
CciI TCATGA 2 cut(s) 510, 630
CfoI GCGC 2 cut(s) 34, 45
Cfr10I RCCGGY 1 cut(s) 149
CseI GACGC 2 cut(s) 70, 148
Csp6I GTAC 1 cut(s) 433
CviAII CATG 4 cut(s) 253, 282, 511, 631
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 3 cut(s) 49, 228, 753
EaeI YGGCCR 2 cut(s) 9, 178
EciI GGCGGA 1 cut(s) 179
Eco130I CCWWGG 3 cut(s) 252, 456, 658
Eco24I GRGCYC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 345, 383
Eco88I CYCGRG 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 3 cut(s) 252, 456, 658
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 3 cut(s) 252, 456, 658
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 4 cut(s) 256, 285, 514, 634
FatI CATG 4 cut(s) 252, 281, 510, 630
Fnu4HI GCNGC 7 cut(s) 18, 54, 154, 157, 570, 585, 641
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 7 cut(s) 18, 54, 154, 157, 570, 585, 641
GlaI GCGC 2 cut(s) 33, 44
GluI GCNGC 7 cut(s) 18, 54, 154, 157, 570, 585, 641
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 11, 180
HapII CCGG 3 cut(s) 150, 177, 209
HgaI GACGC 2 cut(s) 70, 148
HhaI GCGC 2 cut(s) 34, 45
Hin1II CATG 4 cut(s) 256, 285, 514, 634
Hin6I GCGC 2 cut(s) 32, 43
HinP1I GCGC 2 cut(s) 32, 43
HinfI GANTC 2 cut(s) 325, 376
HpaII CCGG 3 cut(s) 150, 177, 209
HphI GGTGA 3 cut(s) 154, 241, 476
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 2 cut(s) 113, 169
Hpy188III TCNNGA 3 cut(s) 299, 511, 631
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 4 cut(s) 154, 204, 369, 407
HpyCH4III ACNGT 1 cut(s) 605
HpyCH4V TGCA 6 cut(s) 281, 383, 497, 593, 650, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 40, 62, 590
HpyF3I CTNAG 3 cut(s) 49, 228, 753
Hsp92II CATG 4 cut(s) 256, 285, 514, 634
HspAI GCGC 2 cut(s) 32, 43
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
LmnI GCTCC 3 cut(s) 61, 192, 286
LpnPI CCDG 9 cut(s) 37, 163, 190, 222, 290, 312, 317, 432, 573
Lsp1109I GCAGC 4 cut(s) 29, 168, 571, 652
LweI GCATC 2 cut(s) 54, 317
MaeIII GTNAC 5 cut(s) 160, 247, 364, 476, 612
MbiI CCGCTC 1 cut(s) 56
MboII GAAGA 1 cut(s) 712
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 67
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 5 cut(s) 330, 386, 439, 470, 645
MluNI TGGCCA 1 cut(s) 11
MmeI TCCRAC 2 cut(s) 153, 192
Mox20I TGGCCA 1 cut(s) 11
MreI CGCCGGCG 1 cut(s) 149
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 604
MscI TGGCCA 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 3 cut(s) 150, 177, 209
MspR9I CCNGG 1 cut(s) 305
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 4 cut(s) 32, 43, 81, 240
MwoI GCNNNNNNNGC 4 cut(s) 17, 40, 62, 590
NaeI GCCGGC 1 cut(s) 151
NcoI CCATGG 1 cut(s) 252
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 4 cut(s) 256, 285, 514, 634
NlaIV GGNNCC 1 cut(s) 656
NmuCI GTSAC 2 cut(s) 160, 247
PagI TCATGA 2 cut(s) 510, 630
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 2 cut(s) 325, 376
PkrI GCNGC 7 cut(s) 19, 55, 155, 158, 571, 586, 642
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 656
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
SatI GCNGC 7 cut(s) 18, 54, 154, 157, 570, 585, 641
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 2 cut(s) 54, 317
SgrAI CRCCGGYG 1 cut(s) 149
Sse9I AATT 5 cut(s) 330, 386, 439, 470, 645
SsiI CCGC 7 cut(s) 15, 54, 79, 153, 190, 240, 570
SspI AATATT 1 cut(s) 534
StyD4I CCNGG 1 cut(s) 303
StyI CCWWGG 3 cut(s) 252, 456, 658
TaaI ACNGT 1 cut(s) 605
TaqI TCGA 1 cut(s) 328
TasI AATT 5 cut(s) 330, 386, 439, 470, 645
TatI WGTACW 1 cut(s) 432
TauI GCSGC 3 cut(s) 56, 156, 572
TfiI GAWTC 2 cut(s) 325, 376
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 2 cut(s) 160, 247
TseI GCWGC 4 cut(s) 17, 156, 584, 640
Tsp45I GTSAC 2 cut(s) 160, 247
TspRI CASTG 1 cut(s) 451
XapI RAATTY 2 cut(s) 386, 439
XmnI GAANNNNTTC 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.