MD04G1052700.v1.1

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009609
Physical Location & Seq
Reverse (-)
6200053 .. 6206259
6207 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1052700.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGAGGTGGCCACCACAGCTTCCAGCTTCGCGCTCCACACGCGCATGTTAGCAACGCCCACCACTCCCCGAGCCCTGAACACTACCCGCCCCCATCTGACGTCGTGTCATTCCCCTACCAAAGCCTCCCTGTCCACCAGCTTCCTCTCTCCCTTCGCCGGCAACAGCCTCTCCTCCGACTTCTCCGGCCACAAGCTCCGCCCTTCCTCCCTCAATCCAGCGTCGTCTCATGGCTCCAAACTCAAACGCGGCGTCGTCACCATGGTTATTCCATTTCAAAGAGGAAGTGCATGGGAGCAGCCGCCTCCGGACCTGGCATCATATCTATACAAAAACCGAATAGTGTACTTGGGCATGTCACTTGTTCCTTCGGTGACGGAGTTGATACTTGCCGAGTTTCTCTACCTTCAGTACGAAGACGACACGAAGCCCATTTACTTGTACATTAATTCCACAGGAACAACCAAGGGTGGGGAGAAGTTGGGTTATGAAACAGAGGCTTTTGCAATATATGATGTCATGAGGTATGTCAAGCCACCTATATTTACTCTTTGTGTTGGTAATGCTTGGGGAGAAGCTGCCTTACTTTTGGCTGCTGGTGCAAAGGGAAACCGTTCTGCTTTACCCTCATCAACAATCATGATGAAGCAGCCGATTGCGAGGTTTCAAGGTCAAGCAACAGATATTGAACTTGCAAGAAAGGAAGTGAGGAATGTTAAAGACGAGTTGGTTAAACTCTTCGCAAAGCATGTGGGAAAGTCAACTGAGCAAATTGAAGCAGACATTAGGCGTCCAAAATATTTTAGTCCAAGTGAGGCAGTTGACTATGGCATCATTGACAAGGTACTGTACAATGAAAGGAGTACCGAAGACAGGGGAGTTGTCTCAGACCTTAAAAAAGCACAACTTATTCCATAG

Protein Analysis

306

Amino Acids

33.58

Weight (kDa)

9.22

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 104 - 284 2.7e-51 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 104
AccII CGCG 3 cut(s) 32, 43, 249
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 4 cut(s) 88, 199, 249, 302
AcoI YGGCCR 2 cut(s) 9, 187
AcuI CTGAAG 1 cut(s) 392
AcyI GRCGYC 3 cut(s) 101, 252, 790
AfaI GTAC 6 cut(s) 347, 413, 443, 846, 851, 865
AfiI CCNNNNNNNGG 3 cut(s) 158, 471, 873
AgsI TTSAA 4 cut(s) 278, 668, 689, 776
AhdI GACNNNNNGTC 1 cut(s) 105
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 5 cut(s) 20, 27, 141, 196, 578
AluI AGCT 5 cut(s) 20, 27, 141, 196, 578
Alw26I GTCTC 2 cut(s) 231, 889
Ama87I CYCGRG 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 2 cut(s) 9, 187
ApeKI GCWGC 4 cut(s) 298, 578, 593, 649
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 613
AspLEI GCGC 2 cut(s) 34, 45
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 2 cut(s) 250, 385
AvaI CYCGRG 1 cut(s) 69
AvaII GGWCC 1 cut(s) 310
BalI TGGCCA 1 cut(s) 11
BanII GRGCYC 1 cut(s) 76
BarI GAAGNNNNNNTAC 4 cut(s) 419, 451, 567, 599
BbsI GAAGAC 2 cut(s) 423, 876
BbvI GCAGC 4 cut(s) 310, 565, 580, 661
BccI CCATC 1 cut(s) 102
BciT130I CCWGG 1 cut(s) 314
BcoDI GTCTC 2 cut(s) 231, 889
BisI GCNGC 6 cut(s) 250, 299, 302, 579, 594, 650
BlsI GCNGC 6 cut(s) 251, 300, 303, 580, 595, 651
Bme1390I CCNGG 1 cut(s) 314
Bme18I GGWCC 1 cut(s) 310
BmeRI GACNNNNNGTC 1 cut(s) 105
BmeT110I CYCGRG 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 2 cut(s) 326, 840
BpiI GAAGAC 2 cut(s) 423, 876
BsaHI GRCGYC 3 cut(s) 101, 252, 790
BsaJI CCNNGG 2 cut(s) 261, 465
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 471, 873
Bse118I RCCGGY 1 cut(s) 158
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 261, 465
BseLI CCNNNNNNNGG 3 cut(s) 158, 471, 873
BseMII CTCAG 2 cut(s) 756, 900
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 4 cut(s) 310, 565, 580, 661
Bsh1236I CGCG 3 cut(s) 32, 43, 249
BshFI GGCC 2 cut(s) 11, 189
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 3 cut(s) 159, 186, 308
BslI CCNNNNNNNGG 3 cut(s) 158, 471, 873
BsmAI GTCTC 2 cut(s) 231, 889
BsmBI CGTCTC 1 cut(s) 231
BsnI GGCC 2 cut(s) 11, 189
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 76
Bsp13I TCCGGA 1 cut(s) 307
Bsp1407I TGTACA 2 cut(s) 441, 849
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 4 cut(s) 88, 199, 249, 302
BspANI GGCC 2 cut(s) 11, 189
BspCNI CTCAG 2 cut(s) 757, 899
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 3 cut(s) 32, 43, 249
BspHI TCATGA 2 cut(s) 519, 639
BspLI GGNNCC 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 158
BsrGI TGTACA 2 cut(s) 441, 849
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 261, 465
BssNI GRCGYC 3 cut(s) 101, 252, 790
BssT1I CCWWGG 2 cut(s) 261, 465
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 614, 849
Bst6I CTCTTC 1 cut(s) 743
BstACI GRCGYC 3 cut(s) 101, 252, 790
BstAUI TGTACA 2 cut(s) 441, 849
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 765, 886
BstDSI CCRYGG 1 cut(s) 261
BstFNI CGCG 3 cut(s) 32, 43, 249
BstHHI GCGC 2 cut(s) 34, 45
BstMAI GTCTC 2 cut(s) 231, 889
BstMWI GCNNNNNNNGC 3 cut(s) 17, 40, 599
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 3 cut(s) 49, 358, 752
BstSCI CCNGG 1 cut(s) 312
BstUI CGCG 3 cut(s) 32, 43, 249
BstV1I GCAGC 4 cut(s) 310, 565, 580, 661
BstV2I GAAGAC 2 cut(s) 423, 876
BsuRI GGCC 2 cut(s) 11, 189
BtgI CCRYGG 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 160
CciI TCATGA 2 cut(s) 519, 639
CfoI GCGC 2 cut(s) 34, 45
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 310
CseI GACGC 3 cut(s) 210, 241, 779
Csp6I GTAC 6 cut(s) 346, 412, 442, 845, 850, 864
CspCI CAANNNNNGTGG 2 cut(s) 732, 767
CviAII CATG 8 cut(s) 46, 230, 262, 291, 355, 520, 640, 749
CviQI GTAC 6 cut(s) 346, 412, 442, 845, 850, 864
DdeI CTNAG 2 cut(s) 765, 886
DriI GACNNNNNGTC 1 cut(s) 105
EaeI YGGCCR 2 cut(s) 9, 187
Eam1104I CTCTTC 1 cut(s) 743
Eam1105I GACNNNNNGTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 743
EciI GGCGGA 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 261, 465
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 2 cut(s) 261, 465
EcoT38I GRGCYC 1 cut(s) 76
ErhI CCWWGG 2 cut(s) 261, 465
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 8 cut(s) 49, 233, 265, 294, 358, 523, 643, 752
FalI AAGNNNNNCTT 1 cut(s) 891
FatI CATG 8 cut(s) 45, 229, 261, 290, 354, 519, 639, 748
FauI CCCGC 1 cut(s) 95
Fnu4HI GCNGC 6 cut(s) 250, 299, 302, 579, 594, 650
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 6 cut(s) 250, 299, 302, 579, 594, 650
GlaI GCGC 2 cut(s) 33, 44
GluI GCNGC 6 cut(s) 250, 299, 302, 579, 594, 650
HaeIII GGCC 2 cut(s) 11, 189
HapII CCGG 3 cut(s) 159, 186, 308
HgaI GACGC 3 cut(s) 210, 241, 779
HhaI GCGC 2 cut(s) 34, 45
Hin1I GRCGYC 3 cut(s) 101, 252, 790
Hin1II CATG 8 cut(s) 49, 233, 265, 294, 358, 523, 643, 752
Hin6I GCGC 2 cut(s) 32, 43
HinP1I GCGC 2 cut(s) 32, 43
HincII GTYRAC 2 cut(s) 762, 823
HindII GTYRAC 2 cut(s) 762, 823
HpaII CCGG 3 cut(s) 159, 186, 308
HphI GGTGA 2 cut(s) 250, 385
Hpy166II GTNNAC 4 cut(s) 135, 346, 762, 823
Hpy188I TCNGA 3 cut(s) 99, 178, 889
Hpy188III TCNNGA 3 cut(s) 308, 520, 640
Hpy8I GTNNAC 4 cut(s) 135, 346, 762, 823
Hpy99I CGWCG 3 cut(s) 106, 226, 257
HpyAV CCTTC 4 cut(s) 163, 213, 378, 416
HpyCH4III ACNGT 2 cut(s) 614, 849
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 4 cut(s) 290, 506, 602, 695
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 40, 599
HpyF3I CTNAG 2 cut(s) 765, 886
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 3 cut(s) 101, 252, 790
Hsp92II CATG 8 cut(s) 49, 233, 265, 294, 358, 523, 643, 752
HspAI GCGC 2 cut(s) 32, 43
Kpn2I TCCGGA 1 cut(s) 307
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
LmnI GCTCC 4 cut(s) 39, 201, 239, 295
Lsp1109I GCAGC 4 cut(s) 310, 565, 580, 661
LweI GCATC 2 cut(s) 326, 840
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 3 cut(s) 256, 357, 373
MboII GAAGA 3 cut(s) 428, 730, 881
MhlI GDGCHC 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 2 cut(s) 448, 771
MluNI TGGCCA 1 cut(s) 11
MmeI TCCRAC 1 cut(s) 201
Mox20I TGGCCA 1 cut(s) 11
MroI TCCGGA 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 613
MscI TGGCCA 1 cut(s) 11
MseI TTAA 4 cut(s) 447, 717, 732, 894
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 3 cut(s) 159, 186, 308
MspR9I CCNGG 1 cut(s) 314
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 3 cut(s) 32, 43, 249
MwoI GCNNNNNNNGC 3 cut(s) 17, 40, 599
NaeI GCCGGC 1 cut(s) 160
NcoI CCATGG 1 cut(s) 261
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 8 cut(s) 49, 233, 265, 294, 358, 523, 643, 752
NlaIV GGNNCC 1 cut(s) 235
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 3 cut(s) 256, 357, 373
NspI RCATGY 3 cut(s) 49, 358, 752
PagI TCATGA 2 cut(s) 519, 639
PdiI GCCGGC 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 613
PkrI GCNGC 6 cut(s) 251, 300, 303, 580, 595, 651
PshBI ATTAAT 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 310
RsaI GTAC 6 cut(s) 347, 413, 443, 846, 851, 865
RsaNI GTAC 6 cut(s) 346, 412, 442, 845, 850, 864
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 4 cut(s) 447, 717, 732, 894
SatI GCNGC 6 cut(s) 250, 299, 302, 579, 594, 650
Sau96I GGNCC 1 cut(s) 310
ScrFI CCNGG 1 cut(s) 314
SduI GDGCHC 1 cut(s) 76
SfaNI GCATC 2 cut(s) 326, 840
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 44
Sse9I AATT 2 cut(s) 448, 771
SsiI CCGC 4 cut(s) 88, 199, 249, 302
SspI AATATT 1 cut(s) 800
StyD4I CCNGG 1 cut(s) 312
StyI CCWWGG 2 cut(s) 261, 465
TaaI ACNGT 2 cut(s) 614, 849
TaiI ACGT 1 cut(s) 104
TasI AATT 2 cut(s) 448, 771
TatI WGTACW 3 cut(s) 345, 441, 849
TauI GCSGC 2 cut(s) 252, 304
Tru1I TTAA 4 cut(s) 447, 717, 732, 894
Tru9I TTAA 4 cut(s) 447, 717, 732, 894
TseFI GTSAC 3 cut(s) 256, 357, 373
TseI GCWGC 4 cut(s) 298, 578, 593, 649
Tsp45I GTSAC 3 cut(s) 256, 357, 373
TspDTI ATGAA 3 cut(s) 504, 659, 870
TspGWI ACGGA 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 310
VspI ATTAAT 1 cut(s) 447
XceI RCATGY 3 cut(s) 49, 358, 752
XmnI GAANNNNTTC 1 cut(s) 613
ZraI GACGTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.