FvH4_5g21170

Ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010416
Physical Location & Seq
Forward (+)
12713721 .. 12716057
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21170.t1

Sequence Viewer

Length: 330 bp
ATGAACCCGGCGGCGAGGAGCGCGTGGAGTTTGTTCCGACGGCCAACGAGTCAGGGAGTGGCGGAGTCACTGAGTCACCACCGTCAGGAGCAAGTGAGAGGGATCCGGGTGAAGGTGCTGAACGGAAACTTGGAGCAAGCGCTGACGTTTATGCAGAGGAAGATGCAGTCCAGTGGGATCGAGAGGATGATCAAGCGCGAGCAGACCCACCACATCAAGAACTCCGAGAAGCGCGTGCTTGCACGCAAGCTTCTCGAGCGTAAGCTCAAGTCCCAGGACCTCGCCCGCAAGCTCAAGGCCATCCTCATCAAGAAAGTCAGAGGCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

110

Amino Acids

12.68

Weight (kDa)

11.77

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 37 - 89 5.4e-11 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 23, 198, 234
AciI CCGC 3 cut(s) 11, 62, 286
AclWI GGATC 3 cut(s) 97, 110, 185
AcoI YGGCCR 1 cut(s) 41
AfeI AGCGCT 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 85, 112
AjnI CCWGG 1 cut(s) 273
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 3 cut(s) 250, 265, 292
AluI AGCT 3 cut(s) 250, 265, 292
AlwI GGATC 3 cut(s) 97, 110, 185
Ama87I CYCGRG 1 cut(s) 254
Aor51HI AGCGCT 1 cut(s) 141
AoxI GGCC 3 cut(s) 41, 297, 322
AspLEI GCGC 4 cut(s) 23, 142, 198, 234
AspS9I GGNCC 1 cut(s) 277
AsuC2I CCSGG 2 cut(s) 8, 107
AsuHPI GGTGA 2 cut(s) 68, 121
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 277
BamHI GGATCC 1 cut(s) 102
BccI CCATC 1 cut(s) 308
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 235, 269
BciT130I CCWGG 1 cut(s) 275
BclI TGATCA 1 cut(s) 189
BcnI CCSGG 2 cut(s) 8, 107
BfoI RGCGCY 1 cut(s) 143
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 3 cut(s) 8, 107, 275
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 3 cut(s) 8, 107, 275
BmsI GCATC 1 cut(s) 153
BpuEI CTTGAG 2 cut(s) 251, 278
BpuMI CCSGG 2 cut(s) 8, 107
BsaJI CCNNGG 1 cut(s) 273
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 112
Bse1I ACTGG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 2 cut(s) 192, 300
BseLI CCNNNNNNNGG 2 cut(s) 85, 112
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 31
Bsh1236I CGCG 3 cut(s) 23, 198, 234
BshFI GGCC 3 cut(s) 43, 299, 324
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 2 cut(s) 8, 106
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 2 cut(s) 85, 112
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 3 cut(s) 43, 299, 324
BsoBI CYCGRG 1 cut(s) 254
Bsp143I GATC 3 cut(s) 102, 177, 189
BspACI CCGC 3 cut(s) 11, 62, 286
BspANI GGCC 3 cut(s) 43, 299, 324
BspCNI CTCAG 1 cut(s) 63
BspFNI CGCG 3 cut(s) 23, 198, 234
BspLI GGNNCC 1 cut(s) 104
BspPI GGATC 3 cut(s) 97, 110, 185
BsrI ACTGG 1 cut(s) 171
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 3 cut(s) 102, 177, 189
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 83
BstC8I GCNNGC 8 cut(s) 138, 200, 236, 240, 244, 248, 286, 290
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 2 cut(s) 192, 300
BstFNI CGCG 3 cut(s) 23, 198, 234
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 4 cut(s) 23, 142, 198, 234
BstKTI GATC 3 cut(s) 105, 180, 192
BstMBI GATC 3 cut(s) 102, 177, 189
BstMWI GCNNNNNNNGC 2 cut(s) 20, 256
BstNI CCWGG 1 cut(s) 275
BstSCI CCNGG 3 cut(s) 6, 105, 273
BstUI CGCG 3 cut(s) 23, 198, 234
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 3 cut(s) 43, 299, 324
BtsCI GGATG 2 cut(s) 192, 300
BtsIMutI CAGTG 2 cut(s) 68, 178
Cac8I GCNNGC 8 cut(s) 138, 200, 236, 240, 244, 248, 286, 290
CfoI GCGC 4 cut(s) 23, 142, 198, 234
Cfr13I GGNCC 1 cut(s) 277
CviJI RGCY 6 cut(s) 43, 250, 265, 292, 299, 324
CviKI_1 RGCY 6 cut(s) 43, 250, 265, 292, 299, 324
DdeI CTNAG 1 cut(s) 71
DpnI GATC 3 cut(s) 104, 179, 191
DpnII GATC 3 cut(s) 102, 177, 189
EaeI YGGCCR 1 cut(s) 41
EciI GGCGGA 1 cut(s) 77
Eco147I AGGCCT 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 277
Eco47III AGCGCT 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 273
FaiI YATR 2 cut(s) 152, 328
FaqI GGGAC 1 cut(s) 256
FauI CCCGC 1 cut(s) 293
FbaI TGATCA 1 cut(s) 189
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 2 cut(s) 199, 287
Fsp4HI GCNGC 1 cut(s) 12
GlaI GCGC 4 cut(s) 22, 141, 197, 233
GluI GCNGC 1 cut(s) 12
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 3 cut(s) 43, 299, 324
HapII CCGG 2 cut(s) 8, 106
HhaI GCGC 4 cut(s) 23, 142, 198, 234
Hin6I GCGC 4 cut(s) 21, 140, 196, 232
HinP1I GCGC 4 cut(s) 21, 140, 196, 232
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 3 cut(s) 49, 65, 73
HpaII CCGG 2 cut(s) 8, 106
HphI GGTGA 2 cut(s) 68, 121
Hpy188I TCNGA 3 cut(s) 38, 226, 320
Hpy188III TCNNGA 5 cut(s) 86, 181, 217, 254, 310
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 3 cut(s) 154, 166, 242
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 256
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 146
HspAI GCGC 4 cut(s) 21, 140, 196, 232
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 3 cut(s) 102, 177, 189
LmnI GCTCC 3 cut(s) 18, 88, 133
LpnPI CCDG 7 cut(s) 21, 38, 71, 119, 184, 260, 287
LweI GCATC 1 cut(s) 153
MaeII ACGT 1 cut(s) 146
MaeIII GTNAC 2 cut(s) 66, 74
MalI GATC 3 cut(s) 104, 179, 191
MboI GATC 3 cut(s) 102, 177, 189
MboII GAAGA 1 cut(s) 172
MflI RGATCY 1 cut(s) 102
MlyI GAGTC 3 cut(s) 58, 74, 82
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 7 cut(s) 9, 92, 150, 177, 290, 314, 314
MspI CCGG 2 cut(s) 8, 106
MspR9I CCNGG 3 cut(s) 8, 107, 275
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 3 cut(s) 23, 198, 234
MwoI GCNNNNNNNGC 2 cut(s) 20, 256
NciI CCSGG 2 cut(s) 8, 107
NdeII GATC 3 cut(s) 102, 177, 189
NlaIV GGNNCC 1 cut(s) 104
NmuCI GTSAC 2 cut(s) 66, 74
PaeR7I CTCGAG 1 cut(s) 254
PceI AGGCCT 1 cut(s) 324
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 3 cut(s) 57, 73, 81
PpsI GAGTC 3 cut(s) 57, 73, 81
PpuMI RGGWCCY 1 cut(s) 277
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 104
PspPI GGNCC 1 cut(s) 277
PspPPI RGGWCCY 1 cut(s) 277
PsuI RGATCY 1 cut(s) 102
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 3 cut(s) 102, 177, 189
Sau96I GGNCC 1 cut(s) 277
SchI GAGTC 3 cut(s) 58, 74, 82
ScrFI CCNGG 3 cut(s) 8, 107, 275
SetI ASST 6 cut(s) 117, 149, 252, 267, 282, 294
SfaNI GCATC 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 3 cut(s) 254, 266, 293
SmoI CTYRAG 3 cut(s) 254, 266, 293
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 3 cut(s) 11, 62, 286
StuI AGGCCT 1 cut(s) 324
StyD4I CCNGG 3 cut(s) 6, 105, 273
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 1 cut(s) 149
TaqI TCGA 2 cut(s) 180, 255
TauI GCSGC 1 cut(s) 14
TscAI CASTG 2 cut(s) 75, 178
TseFI GTSAC 2 cut(s) 66, 74
Tsp45I GTSAC 2 cut(s) 66, 74
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 2 cut(s) 75, 178
VpaK11BI GGWCC 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.