Rroxscaffold_3G00250590

Ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010416
Physical Location & Seq
Reverse (-)
44405585 .. 44406596
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250590.1

Sequence Viewer

Length: 408 bp
ATGAACACGGCGGCGAGGAATGCCTGGAGTTTGTTCCGACGGCCGAGTCAGGGCCTCGCCGAGTCACTGAGTCCGGCGGCTCGCCACCGCCACGAGCAAGTGAGAGGGATTCGCGTGAAGGTTCTGAACGGAAACTTGGAGTGGGCGCTGACGTTTATGCAGAGGAAGATGCAGTCGAGTGGGATCGAGAGGATGATCAAGCGCGAGCAGACCCACCACATCAAGAACTCCGAGAAGCGCGTGTTGGCACGCAAGAACCTCGAGCGTAAGCTCAAATCCCAAGACCTCGCCCGCAAGCTCCAGTCCATCCTCATCAAGAAAGTCAGTTGTTGGGTATACTCGATGAGAATGGGAAAGGAAGAAACAAAGCTTAAGTCCTTTAAACTTCCTGTTGTTGGTTATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

135

Amino Acids

15.82

Weight (kDa)

11.03

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 39 - 91 3e-10 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 45
AccI GTMKAC 1 cut(s) 336
AccII CGCG 3 cut(s) 114, 204, 240
AciI CCGC 4 cut(s) 11, 77, 88, 292
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 50, 395
AflII CTTAAG 1 cut(s) 371
AjnI CCWGG 1 cut(s) 23
AjuI GAANNNNNNNTTGG 4 cut(s) 119, 151, 227, 259
AluBI AGCT 3 cut(s) 271, 298, 370
AluI AGCT 3 cut(s) 271, 298, 370
AlwI GGATC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 2 cut(s) 41, 52
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AspLEI GCGC 3 cut(s) 148, 204, 240
AspS9I GGNCC 1 cut(s) 52
AvaI CYCGRG 1 cut(s) 260
BauI CACGAG 1 cut(s) 92
BccI CCATC 1 cut(s) 314
BceAI ACGGC 2 cut(s) 24, 56
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 25
BclI TGATCA 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 149
BfrI CTTAAG 1 cut(s) 371
BisI GCNGC 2 cut(s) 12, 78
BlsI GCNGC 2 cut(s) 13, 79
Bme1390I CCNGG 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 25
BmsI GCATC 1 cut(s) 159
BpmI CTGGAG 2 cut(s) 46, 284
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 395
Bse1I ACTGG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 25
BseGI GGATG 2 cut(s) 198, 306
BseLI CCNNNNNNNGG 2 cut(s) 50, 395
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 301
BseX3I CGGCCG 1 cut(s) 41
Bsh1236I CGCG 3 cut(s) 114, 204, 240
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 43, 54
BsiEI CGRYCG 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 50, 395
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 2 cut(s) 43, 54
BsoBI CYCGRG 1 cut(s) 260
Bsp143I GATC 2 cut(s) 183, 195
BspACI CCGC 4 cut(s) 11, 77, 88, 292
BspANI GGCC 2 cut(s) 43, 54
BspCNI CTCAG 1 cut(s) 60
BspFNI CGCG 3 cut(s) 114, 204, 240
BspPI GGATC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 371
BsrI ACTGG 1 cut(s) 301
BssMI GATC 2 cut(s) 183, 195
BssNAI GTATAC 1 cut(s) 337
BssSI CACGAG 1 cut(s) 92
Bst1107I GTATAC 1 cut(s) 337
Bst2BI CACGAG 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 25
BstAFI CTTAAG 1 cut(s) 371
BstC8I GCNNGC 5 cut(s) 82, 206, 250, 292, 296
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 198, 306
BstFNI CGCG 3 cut(s) 114, 204, 240
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 148, 204, 240
BstKTI GATC 2 cut(s) 186, 198
BstMBI GATC 2 cut(s) 183, 195
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 23
BstUI CGCG 3 cut(s) 114, 204, 240
BstZ17I GTATAC 1 cut(s) 337
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 2 cut(s) 43, 54
BtsCI GGATG 2 cut(s) 198, 306
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 5 cut(s) 82, 206, 250, 292, 296
CfoI GCGC 3 cut(s) 148, 204, 240
Cfr13I GGNCC 1 cut(s) 52
CviJI RGCY 6 cut(s) 43, 54, 80, 271, 298, 370
CviKI_1 RGCY 6 cut(s) 43, 54, 80, 271, 298, 370
DdeI CTNAG 1 cut(s) 68
DpnI GATC 2 cut(s) 185, 197
DpnII GATC 2 cut(s) 183, 195
DraI TTTAAA 1 cut(s) 382
DrdI GACNNNNNNGTC 1 cut(s) 45
DseDI GACNNNNNNGTC 1 cut(s) 45
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 41
Eco52I CGGCCG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 23
FaiI YATR 3 cut(s) 158, 337, 402
FauI CCCGC 1 cut(s) 299
FbaI TGATCA 1 cut(s) 195
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 12, 78
FokI GGATG 2 cut(s) 205, 293
Fsp4HI GCNGC 2 cut(s) 12, 78
GlaI GCGC 3 cut(s) 147, 203, 239
GluI GCNGC 2 cut(s) 12, 78
GsuI CTGGAG 2 cut(s) 46, 284
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 43, 54
HapII CCGG 1 cut(s) 74
HhaI GCGC 3 cut(s) 148, 204, 240
Hin6I GCGC 3 cut(s) 146, 202, 238
HinP1I GCGC 3 cut(s) 146, 202, 238
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 4 cut(s) 46, 62, 70, 109
HpaII CCGG 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 3 cut(s) 38, 126, 232
Hpy188III TCNNGA 3 cut(s) 187, 223, 316
Hpy8I GTNNAC 1 cut(s) 337
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 160, 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 152
HspAI GCGC 3 cut(s) 146, 202, 238
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 183, 195
LmnI GCTCC 1 cut(s) 303
LpnPI CCDG 6 cut(s) 10, 35, 37, 87, 314, 402
LweI GCATC 1 cut(s) 159
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 63
MalI GATC 2 cut(s) 185, 197
MboI GATC 2 cut(s) 183, 195
MboII GAAGA 2 cut(s) 178, 371
MlyI GAGTC 3 cut(s) 55, 71, 79
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 8 cut(s) 9, 65, 98, 156, 183, 269, 296, 320
MseI TTAA 2 cut(s) 372, 381
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 25
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 3 cut(s) 114, 204, 240
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeII GATC 2 cut(s) 183, 195
NmeAIII GCCGAG 2 cut(s) 69, 85
NmuCI GTSAC 1 cut(s) 63
PaeR7I CTCGAG 1 cut(s) 260
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 2 cut(s) 13, 79
PleI GAGTC 3 cut(s) 54, 70, 78
PpsI GAGTC 3 cut(s) 54, 70, 78
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PspPI GGNCC 1 cut(s) 52
PspXI VCTCGAGB 1 cut(s) 260
SaqAI TTAA 2 cut(s) 372, 381
SatI GCNGC 2 cut(s) 12, 78
Sau3AI GATC 2 cut(s) 183, 195
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 3 cut(s) 55, 71, 79
ScrFI CCNGG 1 cut(s) 25
SetI ASST 7 cut(s) 123, 155, 261, 273, 288, 300, 372
SfaNI GCATC 1 cut(s) 159
Sfr274I CTCGAG 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 260
SmlI CTYRAG 2 cut(s) 260, 371
SmoI CTYRAG 2 cut(s) 260, 371
SsiI CCGC 4 cut(s) 11, 77, 88, 292
StyD4I CCNGG 1 cut(s) 23
TaiI ACGT 1 cut(s) 155
TaqI TCGA 4 cut(s) 176, 186, 261, 341
TauI GCSGC 2 cut(s) 14, 80
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 372, 381
Tru9I TTAA 2 cut(s) 372, 381
TscAI CASTG 1 cut(s) 72
TseFI GTSAC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 63
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 72
Vha464I CTTAAG 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.