MD04G1159200.v1.1

ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010416
Physical Location & Seq
Reverse (-)
24725024 .. 24725416
393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1159200.v1.1.491

Sequence Viewer

Length: 393 bp
ATGAATGCGGTAGCAAGAAGCGCGTGGAAGGTGTTGAAATGGTCCAGTCAGGGCGTGGAGTCAGTGACTCAGTTGGAGTACCATCGTCATGAGCAATTGATAGGGATTTGGGTGAAGGCGATGAACGGGAACTTAGAGTGGGCCCTGACGGTTATGCAAAGGAAGATGACATCGAGGGGGATTGAAAGGATGATAAAGCAAGAGCAAACCCACCACCTCAAAAACTCTGAGAAACTAATCTCGGCTTGCAAGAATCTCGAGCAGAAGCTCCGGTCTCAAGACTTTGCTCGCAAGCTCAAGGCCATCCTCCTCAAGAAAGTCAAAAGCTCTGATTCAGAAATTCTGAACCAAACCCCCCACCGACGCCGTCCCCGCTCACTAAAACTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

131

Amino Acids

15.23

Weight (kDa)

10.57

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 38 - 78 8.3e-06 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 375
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 8, 373
AcsI RAATTY 1 cut(s) 339
AcyI GRCGYC 1 cut(s) 364
AfaI GTAC 1 cut(s) 80
AgsI TTSAA 2 cut(s) 37, 185
AluBI AGCT 3 cut(s) 268, 295, 327
AluI AGCT 3 cut(s) 268, 295, 327
Alw26I GTCTC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 2 cut(s) 141, 300
ApaI GGGCCC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 339
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 3 cut(s) 42, 141, 142
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 145
BccI CCATC 2 cut(s) 90, 311
BceAI ACGGC 1 cut(s) 351
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BcoDI GTCTC 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 3 cut(s) 42, 141, 142
BmiI GGNNCC 1 cut(s) 143
BpuEI CTTGAG 3 cut(s) 261, 281, 296
BsaHI GRCGYC 1 cut(s) 364
BsaI GGTCTC 1 cut(s) 279
BsaWI WCCGGW 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 195, 303
BseMII CTCAG 2 cut(s) 83, 219
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 299
BseSI GKGCMC 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 2 cut(s) 143, 302
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 271
BslFI GGGAC 1 cut(s) 354
BsmAI GTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 143, 302
Bso31I GGTCTC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 145
BspACI CCGC 2 cut(s) 8, 373
BspANI GGCC 2 cut(s) 143, 302
BspCNI CTCAG 2 cut(s) 82, 220
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 375
BsrI ACTGG 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 364
Bst4CI ACNGT 1 cut(s) 151
BstACI GRCGYC 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 247, 289, 293
BstDEI CTNAG 3 cut(s) 69, 133, 228
BstF5I GGATG 2 cut(s) 195, 303
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 23
BstMAI GTCTC 1 cut(s) 279
BstMWI GCNNNNNNNGC 2 cut(s) 20, 372
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 1 cut(s) 23
BsuRI GGCC 2 cut(s) 143, 302
BtgZI GCGATG 1 cut(s) 134
BtsCI GGATG 2 cut(s) 195, 303
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 247, 289, 293
CciI TCATGA 1 cut(s) 88
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 42, 141, 142
CseI GACGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 79
CviAII CATG 1 cut(s) 89
CviJI RGCY 6 cut(s) 143, 245, 268, 295, 302, 327
CviKI_1 RGCY 6 cut(s) 143, 245, 268, 295, 302, 327
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 3 cut(s) 69, 133, 228
Eco24I GRGCYC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 145
FaeI CATG 1 cut(s) 92
FaiI YATR 2 cut(s) 90, 155
FaqI GGGAC 1 cut(s) 354
FatI CATG 1 cut(s) 88
FauI CCCGC 1 cut(s) 380
FokI GGATG 2 cut(s) 202, 290
FriOI GRGCYC 1 cut(s) 145
GlaI GCGC 1 cut(s) 22
HaeIII GGCC 2 cut(s) 143, 302
HapII CCGG 1 cut(s) 271
HgaI GACGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 364
Hin1II CATG 1 cut(s) 92
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HinfI GANTC 4 cut(s) 59, 67, 253, 332
HpaII CCGG 1 cut(s) 271
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 5 cut(s) 229, 331, 337, 345, 392
Hpy188III TCNNGA 4 cut(s) 89, 257, 278, 313
Hpy99I CGWCG 1 cut(s) 366
HpyAV CCTTC 2 cut(s) 22, 109
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 2 cut(s) 157, 249
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 372
HpyF3I CTNAG 3 cut(s) 69, 133, 228
Hsp92I GRCGYC 1 cut(s) 364
Hsp92II CATG 1 cut(s) 92
HspAI GCGC 1 cut(s) 21
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 4 cut(s) 35, 58, 158, 284
MaeIII GTNAC 1 cut(s) 64
MbiI CCGCTC 1 cut(s) 375
MboII GAAGA 1 cut(s) 175
MfeI CAATTG 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 2 cut(s) 95, 339
MlyI GAGTC 2 cut(s) 61, 68
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 4 cut(s) 168, 227, 317, 320
MslI CAYNNNNRTG 1 cut(s) 87
MspI CCGG 1 cut(s) 271
MunI CAATTG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 10
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 20, 372
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 1 cut(s) 64
PaeR7I CTCGAG 1 cut(s) 257
PagI TCATGA 1 cut(s) 88
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 253, 332
PflFI GACNNNGTC 1 cut(s) 366
PleI GAGTC 2 cut(s) 61, 67
PpsI GAGTC 2 cut(s) 61, 67
PspN4I GGNNCC 1 cut(s) 143
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 3 cut(s) 42, 141, 142
PsyI GACNNNGTC 1 cut(s) 366
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 87
Sau96I GGNCC 3 cut(s) 42, 141, 142
SchI GAGTC 2 cut(s) 61, 68
SduI GDGCHC 1 cut(s) 145
SetI ASST 5 cut(s) 33, 219, 270, 297, 329
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 87
SmlI CTYRAG 4 cut(s) 257, 276, 296, 311
SmoI CTYRAG 4 cut(s) 257, 276, 296, 311
Sse9I AATT 2 cut(s) 95, 339
SsiI CCGC 2 cut(s) 8, 373
TaaI ACNGT 1 cut(s) 151
TaqI TCGA 2 cut(s) 173, 258
TasI AATT 2 cut(s) 95, 339
TfiI GAWTC 2 cut(s) 253, 332
TscAI CASTG 1 cut(s) 69
TseFI GTSAC 1 cut(s) 64
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 2 cut(s) 17, 137
TspRI CASTG 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 366
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 1 cut(s) 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XhoI CTCGAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.