MD06G1040200.v1.1

Ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010416
Physical Location & Seq
Reverse (-)
5194404 .. 5196113
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1040200.v1.1.491

Sequence Viewer

Length: 438 bp
ATGAACTCGGTGGCGGCAAGGTGCGCGTGGAACGTGTTGAAACGGGCGAGTCAGGGGGTGGAGTCAGCGACGACGACACTGGGTCATCGACATGAGCAATCGAGAGGGATTCGGGTGAAGGTTATGAACGGGAACCTGGAGTGGGCGCTGACGGTTATGCAGAGGAAGATGACGTCGAGTGGGATCGAGCGGATGATAAAGCGGGAGCAAACCCACCACCTCAAGAACTCAGAGAAGCGCATCTTGGCCCGCAAGAATCTCGAACAGAAACTCCGATCTCAAGACCTCGCTCGCAAGCTCAAGGCCGTCCTCCTCAAGAAAGTCAGGCCTGTGAGAATGTCGTTCGAGCAGATGCTGGATTGGCATTTACCATTTTTTTGGTTCCAGAATTTCACAGCTAAATACAGCACTCTCTTTTCATTTCCGCTGCGACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

146

Amino Acids

17.08

Weight (kDa)

11.33

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 39 - 92 9.1e-10 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 190
AccII CGCG 1 cut(s) 26
AciI CCGC 5 cut(s) 14, 190, 202, 250, 425
AclWI GGATC 1 cut(s) 191
AcsI RAATTY 1 cut(s) 388
AcyI GRCGYC 1 cut(s) 173
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 1 cut(s) 40
AhdI GACNNNNNGTC 1 cut(s) 81
AjnI CCWGG 1 cut(s) 135
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
AluBI AGCT 2 cut(s) 298, 398
AluI AGCT 2 cut(s) 298, 398
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 3 cut(s) 246, 303, 326
ApeKI GCWGC 1 cut(s) 427
ApoI RAATTY 1 cut(s) 388
AspLEI GCGC 3 cut(s) 26, 148, 240
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 127
BbvI GCAGC 1 cut(s) 414
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 434
BfoI RGCGCY 1 cut(s) 149
BisI GCNGC 2 cut(s) 15, 428
BlsI GCNGC 2 cut(s) 16, 429
Bme1390I CCNGG 1 cut(s) 137
BmeRI GACNNNNNGTC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 134, 383
BmrFI CCNGG 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 89
BmsI GCATC 2 cut(s) 249, 342
BmuI ACTGGG 1 cut(s) 89
BpmI CTGGAG 1 cut(s) 158
BpuEI CTTGAG 4 cut(s) 206, 264, 284, 299
BsaHI GRCGYC 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 137
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 84
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 3 cut(s) 248, 305, 328
BslI CCNNNNNNNGG 1 cut(s) 142
BsnI GGCC 3 cut(s) 248, 305, 328
Bsp143I GATC 2 cut(s) 183, 275
BspACI CCGC 5 cut(s) 14, 190, 202, 250, 425
BspANI GGCC 3 cut(s) 248, 305, 328
BspCNI CTCAG 1 cut(s) 242
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 2 cut(s) 134, 383
BspPI GGATC 1 cut(s) 191
BsrBI CCGCTC 1 cut(s) 190
BsrI ACTGG 1 cut(s) 84
BssMI GATC 2 cut(s) 183, 275
BssNI GRCGYC 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 154, 435
BstACI GRCGYC 1 cut(s) 173
BstC8I GCNNGC 3 cut(s) 250, 292, 296
BstDEI CTNAG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 26
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 26, 148, 240
BstKTI GATC 2 cut(s) 186, 278
BstMBI GATC 2 cut(s) 183, 275
BstMWI GCNNNNNNNGC 2 cut(s) 23, 361
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 434
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 1 cut(s) 414
BstXI CCANNNNNNTGG 1 cut(s) 378
BsuRI GGCC 3 cut(s) 248, 305, 328
BtsCI GGATG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 3 cut(s) 250, 292, 296
CaiI CAGNNNCTG 1 cut(s) 355
CfoI GCGC 3 cut(s) 26, 148, 240
Cfr13I GGNCC 1 cut(s) 247
CviAII CATG 1 cut(s) 92
CviJI RGCY 5 cut(s) 248, 298, 305, 328, 398
CviKI_1 RGCY 5 cut(s) 248, 298, 305, 328, 398
DdeI CTNAG 1 cut(s) 229
DpnI GATC 2 cut(s) 185, 277
DpnII GATC 2 cut(s) 183, 275
DriI GACNNNNNGTC 1 cut(s) 81
Eam1105I GACNNNNNGTC 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 135
FaeI CATG 1 cut(s) 95
FaiI YATR 3 cut(s) 93, 125, 158
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FatI CATG 1 cut(s) 91
FauI CCCGC 2 cut(s) 195, 257
Fnu4HI GCNGC 2 cut(s) 15, 428
FokI GGATG 1 cut(s) 205
Fsp4HI GCNGC 2 cut(s) 15, 428
GlaI GCGC 3 cut(s) 25, 147, 239
GluI GCNGC 2 cut(s) 15, 428
GsuI CTGGAG 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 3 cut(s) 248, 305, 328
HhaI GCGC 3 cut(s) 26, 148, 240
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 1 cut(s) 95
Hin6I GCGC 3 cut(s) 24, 146, 238
HinP1I GCGC 3 cut(s) 24, 146, 238
HinfI GANTC 4 cut(s) 49, 62, 109, 256
HphI GGTGA 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 232, 275
Hpy188III TCNNGA 6 cut(s) 102, 223, 260, 281, 316, 385
Hpy99I CGWCG 3 cut(s) 73, 76, 178
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 2 cut(s) 154, 435
HpyCH4IV ACGT 2 cut(s) 33, 173
HpyCH4V TGCA 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 361
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 2 cut(s) 33, 173
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 1 cut(s) 95
HspAI GCGC 3 cut(s) 24, 146, 238
Kzo9I GATC 2 cut(s) 183, 275
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 8 cut(s) 38, 65, 122, 149, 310, 341, 342, 398
Lsp1109I GCAGC 1 cut(s) 414
LweI GCATC 2 cut(s) 249, 342
MaeII ACGT 2 cut(s) 33, 173
MalI GATC 2 cut(s) 185, 277
MbiI CCGCTC 1 cut(s) 190
MboI GATC 2 cut(s) 183, 275
MboII GAAGA 1 cut(s) 178
MluCI AATT 1 cut(s) 388
MlyI GAGTC 2 cut(s) 58, 71
MnlI CCTC 6 cut(s) 98, 156, 230, 296, 320, 323
MslI CAYNNNNRTG 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 427
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 23, 361
NdeII GATC 2 cut(s) 183, 275
NlaIII CATG 1 cut(s) 95
NlaIV GGNNCC 2 cut(s) 134, 383
PceI AGGCCT 1 cut(s) 328
PfeI GAWTC 2 cut(s) 109, 256
PkrI GCNGC 2 cut(s) 16, 429
PleI GAGTC 2 cut(s) 57, 70
PpsI GAGTC 2 cut(s) 57, 70
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 134, 383
PspPI GGNCC 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 90
SatI GCNGC 2 cut(s) 15, 428
Sau3AI GATC 2 cut(s) 183, 275
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 2 cut(s) 58, 71
ScrFI CCNGG 1 cut(s) 137
SetI ASST 9 cut(s) 23, 36, 123, 138, 176, 222, 288, 300, 400
SfaNI GCATC 2 cut(s) 249, 342
SfcI CTRYAG 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 4 cut(s) 221, 279, 299, 314
SmoI CTYRAG 4 cut(s) 221, 279, 299, 314
Sse9I AATT 1 cut(s) 388
SseBI AGGCCT 1 cut(s) 328
SsiI CCGC 5 cut(s) 14, 190, 202, 250, 425
StuI AGGCCT 1 cut(s) 328
StyD4I CCNGG 1 cut(s) 135
TaaI ACNGT 2 cut(s) 154, 435
TaiI ACGT 2 cut(s) 36, 176
TaqI TCGA 6 cut(s) 88, 101, 176, 186, 261, 345
TasI AATT 1 cut(s) 388
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 2 cut(s) 109, 256
TscAI CASTG 1 cut(s) 84
TseI GCWGC 1 cut(s) 427
TspDTI ATGAA 3 cut(s) 17, 140, 408
TspRI CASTG 1 cut(s) 84
XapI RAATTY 1 cut(s) 388
ZraI GACGTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.