RLG00000003229

Ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010416
Physical Location & Seq
Reverse (-)
45869354 .. 45870304
951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003229

Sequence Viewer

Length: 381 bp
ATGAACCCGGCGGTGAGGAATGCCTGGAGTTTGTTCCGACGGCCGAGTCAGGGCCTCGCCGAGTCACTGAGTCCGGCGGCTCACCACCGCCACGAGCAAGTGAGAGGAATTCGCGTGAAGGTTCTGAACGGAAACTTGGAGTGGGCGCTGACGTTTATGCAGAGGAAGATGCAGTCAAGTGGGATCGAGAGGATGATCAAGCGCGAGCAGACCCACCACATCAAGAACTCGGAGAAGCGCGTGTTGGCACGCAAGAACCTCGAGCGTAAGCTCAAATCCCAAGACCTCGCCCGCAAGCTCCAGTCCATCCTCATCAAGAAAGTCAGGAAGTATTTGCCTGAGCACTTCTTTGATGTGCCAGCTGTTGAGTATACTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

127

Amino Acids

14.89

Weight (kDa)

11.16

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 39 - 91 2.6e-10 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 45
AccI GTMKAC 1 cut(s) 371
AccII CGCG 3 cut(s) 114, 204, 240
AciI CCGC 4 cut(s) 11, 77, 88, 292
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 50
AjnI CCWGG 1 cut(s) 23
AjuI GAANNNNNNNTTGG 4 cut(s) 119, 151, 227, 259
AluBI AGCT 3 cut(s) 271, 298, 362
AluI AGCT 3 cut(s) 271, 298, 362
Alw21I GWGCWC 1 cut(s) 345
AlwI GGATC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 2 cut(s) 41, 52
ApoI RAATTY 1 cut(s) 108
AspLEI GCGC 3 cut(s) 148, 204, 240
AspS9I GGNCC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 8
AsuHPI GGTGA 2 cut(s) 25, 74
AvaI CYCGRG 1 cut(s) 260
BauI CACGAG 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 345
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 25
BclI TGATCA 1 cut(s) 195
BcnI CCSGG 1 cut(s) 8
BfoI RGCGCY 1 cut(s) 149
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 2 cut(s) 8, 25
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 52
BmrFI CCNGG 2 cut(s) 8, 25
BmsI GCATC 1 cut(s) 159
BpmI CTGGAG 2 cut(s) 46, 284
Bpu10I CCTNAGC 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 8
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 25
BseGI GGATG 2 cut(s) 198, 306
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 2 cut(s) 59, 330
BseNI ACTGG 1 cut(s) 301
BseX3I CGGCCG 1 cut(s) 41
Bsh1236I CGCG 3 cut(s) 114, 204, 240
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 43, 54
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 2 cut(s) 8, 74
BslI CCNNNNNNNGG 1 cut(s) 50
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 2 cut(s) 43, 54
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 2 cut(s) 183, 195
BspACI CCGC 4 cut(s) 11, 77, 88, 292
BspANI GGCC 2 cut(s) 43, 54
BspCNI CTCAG 2 cut(s) 60, 331
BspFNI CGCG 3 cut(s) 114, 204, 240
BspPI GGATC 1 cut(s) 191
BsrI ACTGG 1 cut(s) 301
BssMI GATC 2 cut(s) 183, 195
BssNAI GTATAC 1 cut(s) 372
BssSI CACGAG 1 cut(s) 92
Bst1107I GTATAC 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 25
BstC8I GCNNGC 5 cut(s) 206, 250, 292, 296, 360
BstDEI CTNAG 2 cut(s) 68, 339
BstF5I GGATG 2 cut(s) 198, 306
BstFNI CGCG 3 cut(s) 114, 204, 240
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 148, 204, 240
BstKTI GATC 2 cut(s) 186, 198
BstMBI GATC 2 cut(s) 183, 195
BstMCI CGRYCG 1 cut(s) 44
BstNI CCWGG 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 6, 23
BstUI CGCG 3 cut(s) 114, 204, 240
BstZ17I GTATAC 1 cut(s) 372
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 2 cut(s) 43, 54
BtsCI GGATG 2 cut(s) 198, 306
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 5 cut(s) 206, 250, 292, 296, 360
CfoI GCGC 3 cut(s) 148, 204, 240
Cfr13I GGNCC 1 cut(s) 52
CviJI RGCY 6 cut(s) 43, 54, 80, 271, 298, 362
CviKI_1 RGCY 6 cut(s) 43, 54, 80, 271, 298, 362
DdeI CTNAG 2 cut(s) 68, 339
DpnI GATC 2 cut(s) 185, 197
DpnII GATC 2 cut(s) 183, 195
DrdI GACNNNNNNGTC 1 cut(s) 45
DseDI GACNNNNNNGTC 1 cut(s) 45
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 41
Eco52I CGGCCG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 23
FaiI YATR 2 cut(s) 158, 372
FauI CCCGC 1 cut(s) 299
FbaI TGATCA 1 cut(s) 195
FblI GTMKAC 1 cut(s) 371
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 2 cut(s) 205, 293
Fsp4HI GCNGC 1 cut(s) 78
GlaI GCGC 3 cut(s) 147, 203, 239
GluI GCNGC 1 cut(s) 78
GsuI CTGGAG 2 cut(s) 46, 284
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 43, 54
HapII CCGG 2 cut(s) 8, 74
HhaI GCGC 3 cut(s) 148, 204, 240
Hin6I GCGC 3 cut(s) 146, 202, 238
HinP1I GCGC 3 cut(s) 146, 202, 238
HinfI GANTC 3 cut(s) 46, 62, 70
HpaII CCGG 2 cut(s) 8, 74
HphI GGTGA 2 cut(s) 25, 74
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 3 cut(s) 38, 126, 232
Hpy188III TCNNGA 4 cut(s) 187, 223, 316, 325
Hpy8I GTNNAC 1 cut(s) 372
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 160, 172
HpyF3I CTNAG 2 cut(s) 68, 339
HpySE526I ACGT 1 cut(s) 152
HspAI GCGC 3 cut(s) 146, 202, 238
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 183, 195
LmnI GCTCC 1 cut(s) 303
LpnPI CCDG 9 cut(s) 10, 21, 35, 37, 87, 310, 314, 351, 372
LweI GCATC 1 cut(s) 159
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 63
MalI GATC 2 cut(s) 185, 197
MboI GATC 2 cut(s) 183, 195
MboII GAAGA 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 1 cut(s) 108
MlyI GAGTC 3 cut(s) 55, 71, 79
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 8 cut(s) 9, 65, 98, 156, 183, 269, 296, 320
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 2 cut(s) 8, 74
MspR9I CCNGG 2 cut(s) 8, 25
Mva1269I GAATGC 1 cut(s) 25
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 3 cut(s) 114, 204, 240
NciI CCSGG 1 cut(s) 8
NdeII GATC 2 cut(s) 183, 195
NmeAIII GCCGAG 2 cut(s) 69, 85
NmuCI GTSAC 1 cut(s) 63
PaeR7I CTCGAG 1 cut(s) 260
PctI GAATGC 1 cut(s) 25
PkrI GCNGC 1 cut(s) 79
PleI GAGTC 3 cut(s) 54, 70, 78
PpsI GAGTC 3 cut(s) 54, 70, 78
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PspPI GGNCC 1 cut(s) 52
PspXI VCTCGAGB 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 362
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 2 cut(s) 183, 195
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 3 cut(s) 55, 71, 79
ScrFI CCNGG 2 cut(s) 8, 25
SduI GDGCHC 1 cut(s) 345
SetI ASST 7 cut(s) 123, 155, 261, 273, 288, 300, 364
SfaNI GCATC 1 cut(s) 159
Sfr274I CTCGAG 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 1 cut(s) 108
SsiI CCGC 4 cut(s) 11, 77, 88, 292
StyD4I CCNGG 2 cut(s) 6, 23
TaiI ACGT 1 cut(s) 155
TaqI TCGA 2 cut(s) 186, 261
TasI AATT 1 cut(s) 108
TauI GCSGC 1 cut(s) 80
TscAI CASTG 1 cut(s) 72
TseFI GTSAC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 63
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 72
XapI RAATTY 1 cut(s) 108
XhoI CTCGAG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.