MD02G1109400.v1.1

Ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010416
Physical Location & Seq
Reverse (-)
8816698 .. 8817030
333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1109400.v1.1.491

Sequence Viewer

Length: 333 bp
ATGGAGTCAGCGACGCCAACACTGGGTCATCGACATGAGCAATCGAGAGGGATTCGGGTGAAGGTTATGAACGGGAACCTGGAGTGGGCGCTGACGGTTATGCAGAGGAAGATGACGTCGAGTGGGATCGAGCGGATGATAAAGCGGCAGCAAACCCACCACCTCAAGAGCTCTGAGAAGCGCATCTTGGCCCGCAAGAATGTCGAACAGAAACTCCGATCTCAAGACCTCGCTCGCAAGCTCAAGGCCGTCCTCCTCAAGAAAGTCAGGTACTCATTTCACAATCTCAAACAACAAATCGCAAAATTATGGATTATACAAGTAGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

111

Amino Acids

12.95

Weight (kDa)

11.06

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 20 - 72 6.8e-10 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 133
AciI CCGC 3 cut(s) 133, 145, 193
AclWI GGATC 1 cut(s) 134
AcyI GRCGYC 2 cut(s) 14, 116
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 23, 85
AjnI CCWGG 1 cut(s) 78
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
AluBI AGCT 2 cut(s) 171, 241
AluI AGCT 2 cut(s) 171, 241
Alw21I GWGCWC 1 cut(s) 173
AlwI GGATC 1 cut(s) 134
AoxI GGCC 2 cut(s) 189, 246
ApeKI GCWGC 1 cut(s) 148
AspLEI GCGC 2 cut(s) 91, 183
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 70
BanII GRGCYC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 160
BceAI ACGGC 1 cut(s) 233
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 2 cut(s) 146, 149
BlsI GCNGC 2 cut(s) 147, 150
Bme1390I CCNGG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 80
BmrI ACTGGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 101
BpuEI CTTGAG 4 cut(s) 149, 207, 227, 242
BsaHI GRCGYC 2 cut(s) 14, 116
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 85
Bse1I ACTGG 1 cut(s) 27
BseBI CCWGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 23, 85
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 2 cut(s) 191, 248
BsiHKAI GWGCWC 1 cut(s) 173
BslI CCNNNNNNNGG 2 cut(s) 23, 85
BsnI GGCC 2 cut(s) 191, 248
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 2 cut(s) 126, 218
BspACI CCGC 3 cut(s) 133, 145, 193
BspANI GGCC 2 cut(s) 191, 248
BspCNI CTCAG 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 77
BspPI GGATC 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 133
BsrI ACTGG 1 cut(s) 27
BssMI GATC 2 cut(s) 126, 218
BssNI GRCGYC 2 cut(s) 14, 116
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 97
BstACI GRCGYC 2 cut(s) 14, 116
BstC8I GCNNGC 3 cut(s) 193, 235, 239
BstDEI CTNAG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 141
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 2 cut(s) 91, 183
BstKTI GATC 2 cut(s) 129, 221
BstMBI GATC 2 cut(s) 126, 218
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 160
BsuRI GGCC 2 cut(s) 191, 248
BtsCI GGATG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 3 cut(s) 193, 235, 239
CfoI GCGC 2 cut(s) 91, 183
Cfr13I GGNCC 1 cut(s) 190
CseI GACGC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 271
CviAII CATG 1 cut(s) 35
CviJI RGCY 4 cut(s) 171, 191, 241, 248
CviKI_1 RGCY 4 cut(s) 171, 191, 241, 248
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 1 cut(s) 174
DpnI GATC 2 cut(s) 128, 220
DpnII GATC 2 cut(s) 126, 218
Ecl136II GAGCTC 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 173
FaeI CATG 1 cut(s) 38
FaiI YATR 5 cut(s) 36, 68, 101, 310, 317
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FatI CATG 1 cut(s) 34
FauI CCCGC 1 cut(s) 200
Fnu4HI GCNGC 2 cut(s) 146, 149
FokI GGATG 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 2 cut(s) 146, 149
GlaI GCGC 2 cut(s) 90, 182
GluI GCNGC 2 cut(s) 146, 149
GsuI CTGGAG 1 cut(s) 101
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 2 cut(s) 191, 248
HgaI GACGC 1 cut(s) 22
HhaI GCGC 2 cut(s) 91, 183
Hin1I GRCGYC 2 cut(s) 14, 116
Hin1II CATG 1 cut(s) 38
Hin6I GCGC 2 cut(s) 89, 181
HinP1I GCGC 2 cut(s) 89, 181
HinfI GANTC 2 cut(s) 5, 52
HphI GGTGA 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 175, 218
Hpy188III TCNNGA 4 cut(s) 45, 166, 224, 259
Hpy99I CGWCG 2 cut(s) 16, 121
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 1 cut(s) 103
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 116
Hsp92I GRCGYC 2 cut(s) 14, 116
Hsp92II CATG 1 cut(s) 38
HspAI GCGC 2 cut(s) 89, 181
Kzo9I GATC 2 cut(s) 126, 218
LpnPI CCDG 4 cut(s) 8, 65, 92, 253
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 1 cut(s) 192
MaeII ACGT 1 cut(s) 116
MalI GATC 2 cut(s) 128, 220
MbiI CCGCTC 1 cut(s) 133
MboI GATC 2 cut(s) 126, 218
MboII GAAGA 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 1 cut(s) 305
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 6 cut(s) 41, 99, 173, 239, 263, 266
MseI TTAA 1 cut(s) 331
MslI CAYNNNNRTG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
NdeII GATC 2 cut(s) 126, 218
NlaIII CATG 1 cut(s) 38
NlaIV GGNNCC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 52
PkrI GCNGC 2 cut(s) 147, 150
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 1 cut(s) 190
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 33
SacI GAGCTC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 331
SatI GCNGC 2 cut(s) 146, 149
Sau3AI GATC 2 cut(s) 126, 218
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 173
SetI ASST 8 cut(s) 66, 81, 119, 165, 173, 231, 243, 272
SfaNI GCATC 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 4 cut(s) 164, 222, 242, 257
SmoI CTYRAG 4 cut(s) 164, 222, 242, 257
Sse9I AATT 1 cut(s) 305
SsiI CCGC 3 cut(s) 133, 145, 193
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 1 cut(s) 119
TaqI TCGA 5 cut(s) 31, 44, 119, 129, 204
TasI AATT 1 cut(s) 305
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 331
Tru9I TTAA 1 cut(s) 331
TscAI CASTG 1 cut(s) 27
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 83
TspRI CASTG 1 cut(s) 27
ZraI GACGTC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.