FvH4_5g21300

tobamovirus multiplication protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012296
Physical Location & Seq
Forward (+)
12832301 .. 12837471
5171 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21300.t1

Sequence Viewer

Length: 816 bp
ATGGCCTGCAGAGGGTGCTTGGTGTTCCTATTGAAGCTATTGAACTTTATATTGACCCTTGTTGGGCTGGCCATGGTGGGCTATGGGATCTACTTGCTGGTAGAATACATGAAAGTAGCCGACACTGCGAATGTGTATTCGCCTGTGGGTGAAACTGACGCTCTGATACAGCTTGGCCGTCCTATGCTCACAACTGTGTCTCTTTCCGCTAGTTTTCTTGACAACTTGCCAAAAGCCTGGTTCATATACTTATTTATTGGGGTTGGAGTTATTATATTTGTCATCTCCTGTTTTGGTTGCATCGGTGCTGTAACACGGAATGGCTGCTGCTTGAGTTGTTATTCGGTTTTGTTGATTCTTTTGATCCTGGTAGAGCTGGCTGCTGCAGCCTTTATATTCTTTGACAAAAGCTGGGAAACCGAAATCCCGACGGATGCGACTGGAGATTTTGAGATGATATATGACTTTCTGGAAGCGCACTGGAGTATTGTCAAATGGGTTGTTCTTGGAGCTGTTATCTTTGAGGCTCTAATTTTCTTGCTAGCCCTTGGAGTTAGAGCAGTAAATAGACCAGTGGAGTATGATAGTGATGAAGAGCCATTAATCAACAGGCCGCCTCCAGTTCCAGCAACCGGTGTACCTGTTGCTGGGGCTGGAAATCAGCGTCCAACCAGAAATGATGCTTGGAGTACACGCATGAGGGAAAAGTACGGGCTGGACACATCTTTGTATCCATATGACCCATCCGATTCAAGCAGGTTACAGCAACCTCAAGCTCAGCCTCAAGAAGAAAGGAGCCGCTGCAGCATCATGTGA

Protein Analysis

272

Amino Acids

29.98

Weight (kDa)

4.74

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 9 - 138 1.9e-16 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32400 AT1G32400 AT1G32400
fragaria_vesca FvH4_5g21300
malus_domestica MD06G1039300.v1.1 MD16G1270100.v1.1
prunus_persica Prupe.5G044200_v2.0.a1 Prupe.5G044200_v2.0.a1 Prupe.5G044200_v2.0.a1
pyrus_communis pycom06g03290 pycom16g23920
rosa_chinensis RchiOBHm_Chr7g0208641
rosa_laevigata RLG00000003206
rosa_roxburghii Rroxscaffold_3G00250360
rosa_rugosa Rorug07G0105100
rosa_samantha Rh7AG240000 Rh7CG254800 Rh7DG245900
rosa_wichuraiana Rw7G020350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 747
AciI CCGC 3 cut(s) 207, 614, 799
AclWI GGATC 2 cut(s) 95, 358
AcoI YGGCCR 2 cut(s) 69, 175
AfaI GTAC 3 cut(s) 639, 691, 710
AfiI CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
AgeI ACCGGT 1 cut(s) 632
AgsI TTSAA 3 cut(s) 34, 43, 753
AjnI CCWGG 2 cut(s) 236, 366
AjuI GAANNNNNNNTTGG 2 cut(s) 667, 699
AleI CACNNNNGTG 1 cut(s) 194
AluBI AGCT 6 cut(s) 37, 172, 376, 411, 512, 776
AluI AGCT 6 cut(s) 37, 172, 376, 411, 512, 776
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 2 cut(s) 95, 358
AoxI GGCC 4 cut(s) 3, 69, 175, 611
ApeKI GCWGC 7 cut(s) 324, 327, 380, 383, 386, 801, 804
AseI ATTAAT 1 cut(s) 602
AsiGI ACCGGT 1 cut(s) 632
AspLEI GCGC 1 cut(s) 478
AsuHPI GGTGA 1 cut(s) 161
AsuNHI GCTAGC 1 cut(s) 541
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BalI TGGCCA 1 cut(s) 71
BbvI GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
BccI CCATC 1 cut(s) 751
BceAI ACGGC 1 cut(s) 162
BciT130I CCWGG 2 cut(s) 238, 368
BciVI GTATCC 1 cut(s) 741
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 2 cut(s) 210, 542
BfmI CTRYAG 3 cut(s) 7, 384, 802
BfuAI ACCTGC 1 cut(s) 747
BfuI GTATCC 1 cut(s) 741
BisI GCNGC 9 cut(s) 325, 328, 381, 384, 387, 614, 799, 802, 805
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 9 cut(s) 326, 329, 382, 385, 388, 615, 800, 803, 806
Bme1390I CCNGG 2 cut(s) 238, 368
BmiI GGNNCC 1 cut(s) 797
BmrFI CCNGG 2 cut(s) 238, 368
BmsI GCATC 3 cut(s) 309, 424, 670
BmtI GCTAGC 1 cut(s) 545
BpmI CTGGAG 3 cut(s) 462, 502, 603
Bpu1102I GCTNAGC 1 cut(s) 777
BpuEI CTTGAG 3 cut(s) 352, 756, 768
BsaJI CCNNGG 2 cut(s) 72, 547
BsaWI WCCGGW 1 cut(s) 632
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 4 cut(s) 445, 485, 572, 620
BseBI CCWGG 2 cut(s) 238, 368
BseDI CCNNGG 2 cut(s) 72, 547
BseGI GGATG 2 cut(s) 439, 743
BseLI CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
BseMII CTCAG 1 cut(s) 791
BseNI ACTGG 4 cut(s) 445, 485, 572, 620
BseXI GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
BseYI CCCAGC 2 cut(s) 411, 647
BshFI GGCC 4 cut(s) 5, 71, 177, 613
BshTI ACCGGT 1 cut(s) 632
BsiSI CCGG 1 cut(s) 633
BslI CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
BsmAI GTCTC 1 cut(s) 204
BsnI GGCC 4 cut(s) 5, 71, 177, 613
Bsp143I GATC 2 cut(s) 87, 363
Bsp1720I GCTNAGC 1 cut(s) 777
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 3 cut(s) 207, 614, 799
BspANI GGCC 4 cut(s) 5, 71, 177, 613
BspCNI CTCAG 1 cut(s) 790
BspLI GGNNCC 1 cut(s) 797
BspMAI CTGCAG 3 cut(s) 11, 388, 806
BspMI ACCTGC 1 cut(s) 747
BspOI GCTAGC 1 cut(s) 545
BspPI GGATC 2 cut(s) 95, 358
BspQI GCTCTTC 1 cut(s) 588
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 4 cut(s) 445, 485, 572, 620
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 2 cut(s) 72, 547
BssMI GATC 2 cut(s) 87, 363
BssT1I CCWWGG 2 cut(s) 72, 547
Bst2UI CCWGG 2 cut(s) 238, 368
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 4 cut(s) 7, 69, 378, 543
BstDEI CTNAG 1 cut(s) 777
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 439, 743
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 2 cut(s) 90, 366
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 2 cut(s) 87, 363
BstMWI GCNNNNNNNGC 4 cut(s) 15, 125, 386, 804
BstNI CCWGG 2 cut(s) 238, 368
BstSCI CCNGG 2 cut(s) 236, 366
BstSFI CTRYAG 3 cut(s) 7, 384, 802
BstV1I GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
BstX2I RGATCY 1 cut(s) 87
BstXI CCANNNNNNTGG 1 cut(s) 237
BstYI RGATCY 1 cut(s) 87
BsuI GTATCC 1 cut(s) 741
BsuRI GGCC 4 cut(s) 5, 71, 177, 613
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 439, 743
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 3 cut(s) 123, 478, 579
BveI ACCTGC 1 cut(s) 747
Cac8I GCNNGC 4 cut(s) 7, 69, 378, 543
CfoI GCGC 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 632
CseI GACGC 2 cut(s) 167, 653
Csp6I GTAC 3 cut(s) 638, 690, 709
CspAI ACCGGT 1 cut(s) 632
CviAII CATG 4 cut(s) 73, 109, 697, 811
CviQI GTAC 3 cut(s) 638, 690, 709
DdeI CTNAG 1 cut(s) 777
DpnI GATC 2 cut(s) 89, 365
DpnII GATC 2 cut(s) 87, 363
EaeI YGGCCR 2 cut(s) 69, 175
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco130I CCWWGG 2 cut(s) 72, 547
EcoRII CCWGG 2 cut(s) 236, 366
EcoT14I CCWWGG 2 cut(s) 72, 547
ErhI CCWWGG 2 cut(s) 72, 547
FaeI CATG 4 cut(s) 76, 112, 700, 814
FatI CATG 4 cut(s) 72, 108, 696, 810
FauNDI CATATG 1 cut(s) 736
Fnu4HI GCNGC 9 cut(s) 325, 328, 381, 384, 387, 614, 799, 802, 805
FokI GGATG 2 cut(s) 446, 730
Fsp4HI GCNGC 9 cut(s) 325, 328, 381, 384, 387, 614, 799, 802, 805
FspBI CTAG 2 cut(s) 210, 542
GlaI GCGC 1 cut(s) 477
GluI GCNGC 9 cut(s) 325, 328, 381, 384, 387, 614, 799, 802, 805
GsaI CCCAGC 2 cut(s) 415, 651
GsuI CTGGAG 3 cut(s) 462, 502, 603
HaeIII GGCC 4 cut(s) 5, 71, 177, 613
HapII CCGG 1 cut(s) 633
HgaI GACGC 2 cut(s) 167, 653
HhaI GCGC 1 cut(s) 478
Hin1II CATG 4 cut(s) 76, 112, 700, 814
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HinfI GANTC 2 cut(s) 355, 749
HpaII CCGG 1 cut(s) 633
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 638, 692
Hpy188I TCNGA 2 cut(s) 165, 748
Hpy188III TCNNGA 4 cut(s) 218, 427, 470, 785
Hpy8I GTNNAC 2 cut(s) 638, 692
Hpy99I CGWCG 1 cut(s) 433
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 4 cut(s) 9, 300, 386, 804
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 125, 386, 804
HpyF3I CTNAG 1 cut(s) 777
Hsp92II CATG 4 cut(s) 76, 112, 700, 814
HspAI GCGC 1 cut(s) 476
Kzo9I GATC 2 cut(s) 87, 363
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 2 cut(s) 509, 795
Lsp1109I GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
LweI GCATC 3 cut(s) 309, 424, 670
MaeI CTAG 2 cut(s) 210, 542
MaeIII GTNAC 2 cut(s) 310, 759
MalI GATC 2 cut(s) 89, 365
MboI GATC 2 cut(s) 87, 363
MboII GAAGA 2 cut(s) 605, 800
MflI RGATCY 1 cut(s) 87
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 1 cut(s) 531
MluNI TGGCCA 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 244, 692
MnlI CCTC 6 cut(s) 5, 517, 627, 693, 780, 792
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MseI TTAA 1 cut(s) 602
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 801
MspI CCGG 1 cut(s) 633
MspR9I CCNGG 2 cut(s) 238, 368
MvaI CCWGG 2 cut(s) 238, 368
MwoI GCNNNNNNNGC 4 cut(s) 15, 125, 386, 804
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 1 cut(s) 736
NdeII GATC 2 cut(s) 87, 363
NheI GCTAGC 1 cut(s) 541
NlaIII CATG 4 cut(s) 76, 112, 700, 814
NlaIV GGNNCC 1 cut(s) 797
OliI CACNNNNGTG 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 355, 749
PinAI ACCGGT 1 cut(s) 632
PkrI GCNGC 9 cut(s) 326, 329, 382, 385, 388, 615, 800, 803, 806
PshBI ATTAAT 1 cut(s) 602
Psp6I CCWGG 2 cut(s) 236, 366
PspFI CCCAGC 2 cut(s) 411, 647
PspGI CCWGG 2 cut(s) 236, 366
PspN4I GGNNCC 1 cut(s) 797
PstI CTGCAG 3 cut(s) 11, 388, 806
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 3 cut(s) 639, 691, 710
RsaNI GTAC 3 cut(s) 638, 690, 709
RseI CAYNNNNRTG 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 9 cut(s) 325, 328, 381, 384, 387, 614, 799, 802, 805
Sau3AI GATC 2 cut(s) 87, 363
ScrFI CCNGG 2 cut(s) 238, 368
SetI ASST 9 cut(s) 39, 174, 378, 413, 514, 643, 761, 772, 778
SfaNI GCATC 3 cut(s) 309, 424, 670
SfcI CTRYAG 3 cut(s) 7, 384, 802
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 3 cut(s) 331, 771, 783
SmoI CTYRAG 3 cut(s) 331, 771, 783
Sse9I AATT 1 cut(s) 531
SsiI CCGC 3 cut(s) 207, 614, 799
SspMI CTAG 2 cut(s) 210, 542
StyD4I CCNGG 2 cut(s) 236, 366
StyI CCWWGG 2 cut(s) 72, 547
TaaI ACNGT 1 cut(s) 196
TasI AATT 1 cut(s) 531
TatI WGTACW 1 cut(s) 689
TauI GCSGC 2 cut(s) 616, 801
TfiI GAWTC 2 cut(s) 355, 749
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 3 cut(s) 130, 485, 579
TseI GCWGC 7 cut(s) 324, 327, 380, 383, 386, 801, 804
TspDTI ATGAA 3 cut(s) 125, 232, 606
TspGWI ACGGA 2 cut(s) 331, 446
TspRI CASTG 3 cut(s) 130, 485, 579
VspI ATTAAT 1 cut(s) 602
XspI CTAG 2 cut(s) 210, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.