Rh7CG254800

tobamovirus multiplication protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0012296
Physical Location & Seq
Forward (+)
24631876 .. 24636021
4146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG254800.1

Sequence Viewer

Length: 816 bp
ATGGCCTGCAGAGGGTGCTTGGAGTGCCTATTGAAGCTATTGAACTTTATATTGACCCTCGTTGGGTTGGCCATTGTGGGTTATGGGATCTACTTGCTGGTAGAATACATGAAAGTAGCCGACAGTGCTAATATGCATTCACCTGTGGGTGAAACTGATGCTCTGATACAGCTTGGCCGCCCTATGCTCACGGCTGTGTCTCTTTCTTCTAATTTTTTTGACAATTTGCCAAAAGCCTGGTTCATATACTTGTTTATTGGGGTTGGAGTCATTATATTTGTCATCTCTTGTTTCGGTTGCATCGGTATTGTAACACGGAATGGCTGCTGCTTGAGTTGTTATTCGGTGTTGTTGATCCTTTTGATCTTGGTAGAGCTGGCTGCTGCAGCCTTTATATTCTTTGACAAAAGCTGGCAAACCGAAATCCCGACAGATGCAACTGGAGATTTTGATATGATATATGAGTTTCTGGAAGCGCACTGGAATATTGTCAAATGGGTTGTTCTTGGTGCTGTTATCTTTGAGGCTCTAATGTTCTTGATAGCCCTCGGAGTTAGAGCTGTAAATAGACCAGTGGAGTATGATAGTGATGAAGAGCCATTGATCAACAGGCCGCCACCAGTTCCAGCAACCGGTGTACCTGTTGCTGGGGCCAGTAGTCAGCGTCCAATTAGAAATGATGCTTGGAGTACACGCATGAGGGAAAAGTACGGGCTCGACACATCTTTGTATCCATATGACCCATCCGATTCAAGCAGGTTCCAGCAACCTCCAGCTCAGCCACAAGAAGAAAGGAGGCGCTGCATCATCATGTGA

Protein Analysis

271

Amino Acids

30.19

Weight (kDa)

4.92

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 8 - 138 5.1e-17 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32400 AT1G32400 AT1G32400
fragaria_vesca FvH4_5g21300
malus_domestica MD06G1039300.v1.1 MD16G1270100.v1.1
prunus_persica Prupe.5G044200_v2.0.a1 Prupe.5G044200_v2.0.a1 Prupe.5G044200_v2.0.a1
pyrus_communis pycom06g03290 pycom16g23920
rosa_chinensis RchiOBHm_Chr7g0208641
rosa_laevigata RLG00000003206
rosa_roxburghii Rroxscaffold_3G00250360
rosa_rugosa Rorug07G0105100
rosa_samantha Rh7AG240000 Rh7CG254800 Rh7DG245900
rosa_wichuraiana Rw7G020350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 747
AciI CCGC 2 cut(s) 178, 614
AclWI GGATC 2 cut(s) 95, 349
AcoI YGGCCR 2 cut(s) 69, 175
AfaI GTAC 3 cut(s) 639, 691, 710
AfiI CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
AgeI ACCGGT 1 cut(s) 632
AgsI TTSAA 3 cut(s) 34, 43, 753
AjnI CCWGG 1 cut(s) 236
AjuI GAANNNNNNNTTGG 2 cut(s) 667, 699
AleI CACNNNNGTG 1 cut(s) 194
AluBI AGCT 6 cut(s) 37, 172, 376, 411, 560, 776
AluI AGCT 6 cut(s) 37, 172, 376, 411, 560, 776
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 2 cut(s) 95, 349
AoxI GGCC 5 cut(s) 3, 69, 175, 611, 651
ApeKI GCWGC 6 cut(s) 324, 327, 380, 383, 386, 801
AsiGI ACCGGT 1 cut(s) 632
AspLEI GCGC 2 cut(s) 478, 801
AspS9I GGNCC 1 cut(s) 651
AsuHPI GGTGA 2 cut(s) 132, 161
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BalI TGGCCA 1 cut(s) 71
BanII GRGCYC 1 cut(s) 717
BbvI GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
BccI CCATC 1 cut(s) 751
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 1 cut(s) 238
BciVI GTATCC 1 cut(s) 741
BclI TGATCA 1 cut(s) 603
BcoDI GTCTC 1 cut(s) 204
BfmI CTRYAG 2 cut(s) 7, 384
BfoI RGCGCY 1 cut(s) 802
BfuAI ACCTGC 1 cut(s) 747
BfuI GTATCC 1 cut(s) 741
BisI GCNGC 8 cut(s) 178, 325, 328, 381, 384, 387, 614, 802
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 8 cut(s) 179, 326, 329, 382, 385, 388, 615, 803
Bme1390I CCNGG 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 651
BmiI GGNNCC 2 cut(s) 652, 761
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 4 cut(s) 148, 309, 424, 670
BpmI CTGGAG 2 cut(s) 462, 756
Bpu1102I GCTNAGC 1 cut(s) 777
BpuEI CTTGAG 1 cut(s) 352
BsaJI CCNNGG 1 cut(s) 547
BsaWI WCCGGW 1 cut(s) 632
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 5 cut(s) 445, 485, 572, 620, 654
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 547
BseGI GGATG 1 cut(s) 743
BseLI CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
BseMII CTCAG 1 cut(s) 791
BseNI ACTGG 5 cut(s) 445, 485, 572, 620, 654
BseXI GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
BseYI CCCAGC 1 cut(s) 647
BshFI GGCC 5 cut(s) 5, 71, 177, 613, 653
BshTI ACCGGT 1 cut(s) 632
BsiSI CCGG 1 cut(s) 633
BslI CCNNNNNNNGG 4 cut(s) 12, 63, 632, 647
BsmAI GTCTC 1 cut(s) 204
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 5 cut(s) 5, 71, 177, 613, 653
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 4 cut(s) 87, 354, 363, 603
Bsp1720I GCTNAGC 1 cut(s) 777
BspACI CCGC 2 cut(s) 178, 614
BspANI GGCC 5 cut(s) 5, 71, 177, 613, 653
BspCNI CTCAG 1 cut(s) 790
BspLI GGNNCC 2 cut(s) 652, 761
BspMAI CTGCAG 2 cut(s) 11, 388
BspMI ACCTGC 1 cut(s) 747
BspPI GGATC 2 cut(s) 95, 349
BspQI GCTCTTC 1 cut(s) 588
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 5 cut(s) 445, 485, 572, 620, 654
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 1 cut(s) 547
BssMI GATC 4 cut(s) 87, 354, 363, 603
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 3 cut(s) 7, 378, 413
BstDEI CTNAG 1 cut(s) 777
BstF5I GGATG 1 cut(s) 743
BstH2I RGCGCY 1 cut(s) 802
BstHHI GCGC 2 cut(s) 478, 801
BstKTI GATC 4 cut(s) 90, 357, 366, 606
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 4 cut(s) 87, 354, 363, 603
BstMWI GCNNNNNNNGC 4 cut(s) 15, 24, 125, 386
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstSFI CTRYAG 2 cut(s) 7, 384
BstV1I GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
BstX2I RGATCY 1 cut(s) 87
BstXI CCANNNNNNTGG 1 cut(s) 237
BstYI RGATCY 1 cut(s) 87
BsuI GTATCC 1 cut(s) 741
BsuRI GGCC 5 cut(s) 5, 71, 177, 613, 653
BtsCI GGATG 1 cut(s) 743
BtsIMutI CAGTG 3 cut(s) 130, 478, 579
BveI ACCTGC 1 cut(s) 747
Cac8I GCNNGC 3 cut(s) 7, 378, 413
CfoI GCGC 2 cut(s) 478, 801
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 1 cut(s) 651
CseI GACGC 1 cut(s) 653
Csp6I GTAC 3 cut(s) 638, 690, 709
CspAI ACCGGT 1 cut(s) 632
CviAII CATG 3 cut(s) 109, 697, 811
CviQI GTAC 3 cut(s) 638, 690, 709
DdeI CTNAG 1 cut(s) 777
DpnI GATC 4 cut(s) 89, 356, 365, 605
DpnII GATC 4 cut(s) 87, 354, 363, 603
EaeI YGGCCR 2 cut(s) 69, 175
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco24I GRGCYC 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 236
EcoT22I ATGCAT 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 717
FaeI CATG 3 cut(s) 112, 700, 814
FatI CATG 3 cut(s) 108, 696, 810
FauNDI CATATG 1 cut(s) 736
FbaI TGATCA 1 cut(s) 603
Fnu4HI GCNGC 8 cut(s) 178, 325, 328, 381, 384, 387, 614, 802
FokI GGATG 1 cut(s) 730
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 8 cut(s) 178, 325, 328, 381, 384, 387, 614, 802
GlaI GCGC 2 cut(s) 477, 800
GluI GCNGC 8 cut(s) 178, 325, 328, 381, 384, 387, 614, 802
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 2 cut(s) 462, 756
HaeII RGCGCY 1 cut(s) 802
HaeIII GGCC 5 cut(s) 5, 71, 177, 613, 653
HapII CCGG 1 cut(s) 633
HgaI GACGC 1 cut(s) 653
HhaI GCGC 2 cut(s) 478, 801
Hin1II CATG 3 cut(s) 112, 700, 814
Hin6I GCGC 2 cut(s) 476, 799
HinP1I GCGC 2 cut(s) 476, 799
HinfI GANTC 2 cut(s) 267, 749
HpaII CCGG 1 cut(s) 633
HphI GGTGA 2 cut(s) 132, 161
Hpy166II GTNNAC 2 cut(s) 638, 692
Hpy188I TCNGA 3 cut(s) 165, 551, 748
Hpy188III TCNNGA 3 cut(s) 427, 470, 538
Hpy8I GTNNAC 2 cut(s) 638, 692
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4V TGCA 6 cut(s) 9, 136, 300, 386, 437, 804
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 24, 125, 386
HpyF3I CTNAG 1 cut(s) 777
Hsp92II CATG 3 cut(s) 112, 700, 814
HspAI GCGC 2 cut(s) 476, 799
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 4 cut(s) 87, 354, 363, 603
LguI GCTCTTC 1 cut(s) 588
Lsp1109I GCAGC 6 cut(s) 311, 314, 367, 370, 398, 788
LweI GCATC 4 cut(s) 148, 309, 424, 670
MaeIII GTNAC 1 cut(s) 310
MalI GATC 4 cut(s) 89, 356, 365, 605
MboI GATC 4 cut(s) 87, 354, 363, 603
MboII GAAGA 3 cut(s) 198, 605, 800
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 717
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 3 cut(s) 211, 223, 669
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 7 cut(s) 5, 68, 517, 557, 693, 780, 789
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 1 cut(s) 138
MscI TGGCCA 1 cut(s) 71
MslI CAYNNNNRTG 2 cut(s) 194, 809
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 1 cut(s) 633
MspR9I CCNGG 1 cut(s) 238
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 4 cut(s) 15, 24, 125, 386
NdeI CATATG 1 cut(s) 736
NdeII GATC 4 cut(s) 87, 354, 363, 603
NlaIII CATG 3 cut(s) 112, 700, 814
NlaIV GGNNCC 2 cut(s) 652, 761
NsiI ATGCAT 1 cut(s) 138
OliI CACNNNNGTG 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 588
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 749
PinAI ACCGGT 1 cut(s) 632
PkrI GCNGC 8 cut(s) 179, 326, 329, 382, 385, 388, 615, 803
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 236
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 652, 761
PspPI GGNCC 1 cut(s) 651
PstI CTGCAG 2 cut(s) 11, 388
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 3 cut(s) 639, 691, 710
RsaNI GTAC 3 cut(s) 638, 690, 709
RseI CAYNNNNRTG 2 cut(s) 194, 809
SapI GCTCTTC 1 cut(s) 588
SatI GCNGC 8 cut(s) 178, 325, 328, 381, 384, 387, 614, 802
Sau3AI GATC 4 cut(s) 87, 354, 363, 603
Sau96I GGNCC 1 cut(s) 651
SchI GAGTC 1 cut(s) 276
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 1 cut(s) 717
SfaNI GCATC 4 cut(s) 148, 309, 424, 670
SfcI CTRYAG 2 cut(s) 7, 384
SmiMI CAYNNNNRTG 2 cut(s) 194, 809
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
Sse9I AATT 3 cut(s) 211, 223, 669
SsiI CCGC 2 cut(s) 178, 614
SspI AATATT 1 cut(s) 487
StyD4I CCNGG 1 cut(s) 236
TaaI ACNGT 1 cut(s) 125
TaqI TCGA 1 cut(s) 717
TasI AATT 3 cut(s) 211, 223, 669
TatI WGTACW 1 cut(s) 689
TauI GCSGC 2 cut(s) 180, 616
TfiI GAWTC 1 cut(s) 749
TscAI CASTG 3 cut(s) 130, 485, 579
TseI GCWGC 6 cut(s) 324, 327, 380, 383, 386, 801
TspDTI ATGAA 3 cut(s) 125, 232, 606
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 3 cut(s) 130, 485, 579
Zsp2I ATGCAT 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.