MD06G1039300.v1.1

Tobamovirus multiplication protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012296
Physical Location & Seq
Reverse (-)
5005160 .. 5016980
11821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1039300.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGCCTGCAGGGGGTGCTTGGAGTGCCTGTTGAAGCTGTTGAACTTTGTTTTGACCCTTGTGGGGCTGGCCATGGTGGGCTATGGGATCTACTTGCTGGTCGAATACTTGAGAGCTTCGGACTCAACCGTTATGTCTTCACCTGTAGGCGACGATCATGATTTGGTACAGTTGAGCCGACCTTTGCTCATGACTGTGTCTCTTTCAACTAGTGTCTTGGATAATTTGCCAAAAGCTTGGTTCATATACTTGTTTATTGGCATTGGAGCGATTATATTTATCATCTCTTGTTTTGGTTGCGTGGGTGCTATGACACGGAATGGCTGCTGCCTGAGTTGTTATTCGGTCCTGGTGATCCTTTTGATCTTGGTAGAGCTGGGAGCTGCCGCCTTTATATTCTTTGACAAAAGCTGGGAAGATGAAATACCAACGGATTCAAGTGGAGATTTTGAGATGATATATGCATTTCTGAAAGCTCACTGGACCATTGTTAGATGGGTTGCTCTTGGAGCTGTTGTCTTCGAGGCTCTACTTTTCTTGCTAGCTCTAGTGGTTAGGGCAGCAAACAGACCAGCAGAATATGACAGTGATGATGAGCTCATTGCTCCAAGGCAACAAATTAGGCAGCCATTGATTGCCAACAGGCAAGCTGTTCCGGCAACTGGTGCACCTGTTGCTGGGACTCTTGATCAGCGACCACCAAGCAGAAATGATGCGTGGAGTACACGCATGAGGGAAAAGTACGGGCTTGATACAGCTGAGTTTACATACAACCCATCAGAGTCACAGAGGCAAGTTTCAGCTCAACCACAAGAAGAAAGGAGCCGATGCATCATCATGTGA

Protein Analysis

281

Amino Acids

31.03

Weight (kDa)

4.94

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 8 - 138 6.5e-17 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32400 AT1G32400 AT1G32400
fragaria_vesca FvH4_5g21300
malus_domestica MD06G1039300.v1.1 MD16G1270100.v1.1
prunus_persica Prupe.5G044200_v2.0.a1 Prupe.5G044200_v2.0.a1 Prupe.5G044200_v2.0.a1
pyrus_communis pycom06g03290 pycom16g23920
rosa_chinensis RchiOBHm_Chr7g0208641
rosa_laevigata RLG00000003206
rosa_roxburghii Rroxscaffold_3G00250360
rosa_rugosa Rorug07G0105100
rosa_samantha Rh7AG240000 Rh7CG254800 Rh7DG245900
rosa_wichuraiana Rw7G020350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 387
AclWI GGATC 2 cut(s) 95, 349
AcoI YGGCCR 1 cut(s) 69
AfaI GTAC 3 cut(s) 168, 724, 743
AfiI CCNNNNNNNGG 4 cut(s) 12, 63, 662, 677
AgsI TTSAA 4 cut(s) 34, 43, 207, 438
AhlI ACTAGT 1 cut(s) 209
AjnI CCWGG 1 cut(s) 348
Alw21I GWGCWC 2 cut(s) 600, 670
Alw26I GTCTC 1 cut(s) 204
Alw44I GTGCAC 1 cut(s) 666
AlwI GGATC 2 cut(s) 95, 349
AoxI GGCC 2 cut(s) 3, 69
ApaLI GTGCAC 1 cut(s) 666
ApeKI GCWGC 5 cut(s) 324, 327, 383, 560, 625
AspS9I GGNCC 2 cut(s) 346, 483
AsuHPI GGTGA 2 cut(s) 132, 364
AsuNHI GCTAGC 1 cut(s) 541
AvaII GGWCC 2 cut(s) 346, 483
BaeGI GKGCMC 1 cut(s) 670
BalI TGGCCA 1 cut(s) 71
BanII GRGCYC 1 cut(s) 600
BarI GAAGNNNNNNTAC 2 cut(s) 408, 440
BbsI GAAGAC 2 cut(s) 129, 511
Bbv12I GWGCWC 2 cut(s) 600, 670
BbvI GCAGC 5 cut(s) 311, 314, 370, 572, 637
BccI CCATC 2 cut(s) 489, 784
BciT130I CCWGG 1 cut(s) 350
BclI TGATCA 1 cut(s) 688
BcoDI GTCTC 1 cut(s) 204
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 3 cut(s) 210, 542, 548
BfmI CTRYAG 2 cut(s) 7, 144
BisI GCNGC 6 cut(s) 325, 328, 384, 387, 561, 626
BlsI GCNGC 6 cut(s) 326, 329, 385, 388, 562, 627
Bme1390I CCNGG 1 cut(s) 350
Bme18I GGWCC 2 cut(s) 346, 483
BmgT120I GGNCC 2 cut(s) 346, 483
BmiI GGNNCC 1 cut(s) 824
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 2 cut(s) 703, 818
BmtI GCTAGC 1 cut(s) 545
BpiI GAAGAC 2 cut(s) 129, 511
BpuEI CTTGAG 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 72, 608
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 501, 531
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 63, 662, 677
Bse1I ACTGG 2 cut(s) 485, 667
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 2 cut(s) 72, 608
BseLI CCNNNNNNNGG 4 cut(s) 12, 63, 662, 677
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 2 cut(s) 323, 750
BseNI ACTGG 2 cut(s) 485, 667
BseSI GKGCMC 1 cut(s) 670
BseXI GCAGC 5 cut(s) 311, 314, 370, 572, 637
BseYI CCCAGC 3 cut(s) 376, 411, 677
BshFI GGCC 2 cut(s) 5, 71
BsiHKAI GWGCWC 2 cut(s) 600, 670
BsiSI CCGG 1 cut(s) 656
BslFI GGGAC 1 cut(s) 694
BslI CCNNNNNNNGG 4 cut(s) 12, 63, 662, 677
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 694
BsnI GGCC 2 cut(s) 5, 71
Bsp1286I GDGCHC 2 cut(s) 600, 670
Bsp143I GATC 5 cut(s) 87, 154, 354, 363, 688
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 1 cut(s) 387
BspANI GGCC 2 cut(s) 5, 71
BspCNI CTCAG 2 cut(s) 324, 751
BspHI TCATGA 2 cut(s) 157, 189
BspLI GGNNCC 1 cut(s) 824
BspMAI CTGCAG 1 cut(s) 11
BspOI GCTAGC 1 cut(s) 545
BspPI GGATC 2 cut(s) 95, 349
BsrDI GCAATG 1 cut(s) 600
BsrI ACTGG 2 cut(s) 485, 667
BssECI CCNNGG 2 cut(s) 72, 608
BssMI GATC 5 cut(s) 87, 154, 354, 363, 688
BssT1I CCWWGG 2 cut(s) 72, 608
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 4 cut(s) 130, 171, 196, 587
BstAPI GCANNNNNTGC 3 cut(s) 15, 665, 674
BstC8I GCNNGC 4 cut(s) 7, 69, 543, 648
BstDEI CTNAG 2 cut(s) 332, 759
BstDSI CCRYGG 1 cut(s) 72
BstKTI GATC 5 cut(s) 90, 157, 357, 366, 691
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 5 cut(s) 87, 154, 354, 363, 688
BstMWI GCNNNNNNNGC 6 cut(s) 15, 24, 509, 656, 665, 674
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 348
BstSFI CTRYAG 2 cut(s) 7, 144
BstSLI GKGCMC 1 cut(s) 670
BstV1I GCAGC 5 cut(s) 311, 314, 370, 572, 637
BstV2I GAAGAC 2 cut(s) 129, 511
BstX2I RGATCY 1 cut(s) 87
BstXI CCANNNNNNTGG 1 cut(s) 237
BstYI RGATCY 1 cut(s) 87
BsuRI GGCC 2 cut(s) 5, 71
BtgI CCRYGG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 478, 592
Cac8I GCNNGC 4 cut(s) 7, 69, 543, 648
CciI TCATGA 2 cut(s) 157, 189
Cfr13I GGNCC 2 cut(s) 346, 483
Csp6I GTAC 3 cut(s) 167, 723, 742
CviAII CATG 5 cut(s) 73, 158, 190, 730, 838
CviQI GTAC 3 cut(s) 167, 723, 742
DdeI CTNAG 2 cut(s) 332, 759
DpnI GATC 5 cut(s) 89, 156, 356, 365, 690
DpnII GATC 5 cut(s) 87, 154, 354, 363, 688
EaeI YGGCCR 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 72, 608
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 346, 483
Eco53kI GAGCTC 1 cut(s) 598
EcoICRI GAGCTC 1 cut(s) 598
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 2 cut(s) 72, 608
EcoT22I ATGCAT 2 cut(s) 466, 833
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 2 cut(s) 72, 608
FaeI CATG 5 cut(s) 76, 161, 193, 733, 841
FaqI GGGAC 1 cut(s) 694
FatI CATG 5 cut(s) 72, 157, 189, 729, 837
FbaI TGATCA 1 cut(s) 688
Fnu4HI GCNGC 6 cut(s) 325, 328, 384, 387, 561, 626
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 6 cut(s) 325, 328, 384, 387, 561, 626
FspBI CTAG 3 cut(s) 210, 542, 548
GluI GCNGC 6 cut(s) 325, 328, 384, 387, 561, 626
GsaI CCCAGC 3 cut(s) 380, 415, 681
HaeIII GGCC 2 cut(s) 5, 71
HapII CCGG 1 cut(s) 656
Hin1II CATG 5 cut(s) 76, 161, 193, 733, 841
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 4 cut(s) 122, 434, 682, 782
HpaII CCGG 1 cut(s) 656
HphI GGTGA 2 cut(s) 132, 364
Hpy166II GTNNAC 3 cut(s) 668, 725, 765
Hpy188I TCNGA 3 cut(s) 121, 471, 781
Hpy188III TCNNGA 3 cut(s) 158, 190, 686
Hpy8I GTNNAC 3 cut(s) 668, 725, 765
Hpy99I CGWCG 1 cut(s) 155
HpyCH4III ACNGT 4 cut(s) 130, 171, 196, 587
HpyCH4V TGCA 4 cut(s) 9, 464, 668, 831
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 24, 509, 656, 665, 674
HpyF3I CTNAG 2 cut(s) 332, 759
Hsp92II CATG 5 cut(s) 76, 161, 193, 733, 841
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 5 cut(s) 87, 154, 354, 363, 688
LmnI GCTCC 5 cut(s) 266, 380, 509, 610, 822
Lsp1109I GCAGC 5 cut(s) 311, 314, 370, 572, 637
LweI GCATC 2 cut(s) 703, 818
MaeI CTAG 3 cut(s) 210, 542, 548
MaeIII GTNAC 1 cut(s) 783
MalI GATC 5 cut(s) 89, 156, 356, 365, 690
MboI GATC 5 cut(s) 87, 154, 354, 363, 688
MboII GAAGA 4 cut(s) 129, 428, 511, 827
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 2 cut(s) 600, 670
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 2 cut(s) 223, 618
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 3 cut(s) 116, 676, 791
MnlI CCTC 3 cut(s) 517, 726, 783
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 2 cut(s) 466, 833
MscI TGGCCA 1 cut(s) 71
MslI CAYNNNNRTG 2 cut(s) 194, 836
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 1 cut(s) 656
MspR9I CCNGG 1 cut(s) 350
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 6 cut(s) 15, 24, 509, 656, 665, 674
NcoI CCATGG 1 cut(s) 72
NdeII GATC 5 cut(s) 87, 154, 354, 363, 688
NheI GCTAGC 1 cut(s) 541
NlaIII CATG 5 cut(s) 76, 161, 193, 733, 841
NlaIV GGNNCC 1 cut(s) 824
NmuCI GTSAC 1 cut(s) 783
NsiI ATGCAT 2 cut(s) 466, 833
PagI TCATGA 2 cut(s) 157, 189
PfeI GAWTC 1 cut(s) 434
PflFI GACNNNGTC 1 cut(s) 196
PkrI GCNGC 6 cut(s) 326, 329, 385, 388, 562, 627
PleI GAGTC 3 cut(s) 116, 676, 790
PpsI GAGTC 3 cut(s) 116, 676, 790
Psp124BI GAGCTC 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 348
PspFI CCCAGC 3 cut(s) 376, 411, 677
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 824
PspPI GGNCC 2 cut(s) 346, 483
PstI CTGCAG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 87
PsyI GACNNNGTC 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 3 cut(s) 168, 724, 743
RsaNI GTAC 3 cut(s) 167, 723, 742
RseI CAYNNNNRTG 2 cut(s) 194, 836
SacI GAGCTC 1 cut(s) 600
SatI GCNGC 6 cut(s) 325, 328, 384, 387, 561, 626
Sau3AI GATC 5 cut(s) 87, 154, 354, 363, 688
Sau96I GGNCC 2 cut(s) 346, 483
SbfI CCTGCAGG 1 cut(s) 11
SchI GAGTC 3 cut(s) 116, 676, 791
ScrFI CCNGG 1 cut(s) 350
SdaI CCTGCAGG 1 cut(s) 11
SduI GDGCHC 2 cut(s) 600, 670
SfaNI GCATC 2 cut(s) 703, 818
SfcI CTRYAG 2 cut(s) 7, 144
SinI GGWCC 2 cut(s) 346, 483
SmiMI CAYNNNNRTG 2 cut(s) 194, 836
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
SpeI ACTAGT 1 cut(s) 209
Sse8387I CCTGCAGG 1 cut(s) 11
Sse9I AATT 2 cut(s) 223, 618
SsiI CCGC 1 cut(s) 387
SspMI CTAG 3 cut(s) 210, 542, 548
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 2 cut(s) 72, 608
TaaI ACNGT 4 cut(s) 130, 171, 196, 587
TaqI TCGA 2 cut(s) 102, 522
TaqII GACCGA 1 cut(s) 334
TasI AATT 2 cut(s) 223, 618
TatI WGTACW 1 cut(s) 722
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 1 cut(s) 434
TscAI CASTG 2 cut(s) 485, 592
TseFI GTSAC 1 cut(s) 783
TseI GCWGC 5 cut(s) 324, 327, 383, 560, 625
Tsp45I GTSAC 1 cut(s) 783
TspDTI ATGAA 2 cut(s) 232, 435
TspGWI ACGGA 2 cut(s) 331, 446
TspRI CASTG 2 cut(s) 485, 592
Tth111I GACNNNGTC 1 cut(s) 196
VneI GTGCAC 1 cut(s) 666
VpaK11BI GGWCC 2 cut(s) 346, 483
XspI CTAG 3 cut(s) 210, 542, 548
Zsp2I ATGCAT 2 cut(s) 466, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.