FvH4_5g24000

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012348
Physical Location & Seq
Forward (+)
15273743 .. 15275504
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24000.t1

Sequence Viewer

Length: 747 bp
ATGGGAAATCGACGGAAGGGGAGAGGCGGAGGAGGAGGAGAAGAGGAGGAGCTTCACGCGGCGGCGAGGTCGGGAGACCTGAGTCAAGTTCAGGTGATTGTCGGCTCCAACCCTTTGAAGGTCAATGGAAGAGATAAGCACTCCAGAACTCCACTACATTTGGCTGCATGGGCTGGGCAAACAGAAGTGGTGAATTTTCTGTGTAAGAACAAGGCTGATGTCGGTGCTGCTGCTATGGATGACATGAAGGCAATACACTTTGCTGCGCAAAAGGGACATGTAGAGGTTGTTCGGGCGCTACTTTCTTCTGGAGCTTCGGTCAAGTCTTCCACTCGCAAAGGTCTCACTCCACTGCACTTTGCTGTTCAAGGATGCCATGTGGAACTTATCAAGTATTTGGCTAGGAAAGGTGCGGATCTCACCACAAAGACAAAAGCAGGGAAGACCCCTCTTGAGCTTGCTAGCAATGATGAAGTCCGCTCTTGTTTGGAAGAGTGTGAAAGGTCATCTGAGAAAGGAGATCTGAATGTTAAACAGAAAGATGAAGAATCTGATCCAAAGATAATCCAGCAAGAAGATATGAAATCTGGAGGTGAAGCGCCGGGTTCTGGGAATGATGAACATGCCAAGGATGAAAGTCTGAAGAGGAAGGGTGATTCTGGGGAGGCCTCGGGGGAACCAAAAAGATCCAAAGTTTCACTGAACCATCTCCTGGCTGCTGATGATGATATACAGGATGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5

Protein Analysis

249

Amino Acids

26.53

Weight (kDa)

6.17

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 75 1.2e-08 Ankyrin repeats (3 copies)
Ank_4 PF13637 18 - 67 3.7e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 38 - 75 2.5e-06 Ankyrin repeats (many copies)
Ank PF00023 49 - 76 2.2e-06 Ankyrin repeat
Ank_2 PF12796 85 - 156 1.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 85 - 133 3.1e-12 Ankyrin repeats (many copies)
Ank_5 PF13857 105 - 154 1.4e-07 Ankyrin repeats (many copies)
Ank PF00023 114 - 144 6.9e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19150 AT4G19150
fragaria_vesca FvH4_5g24000
prunus_persica Prupe.5G019900_v2.0.a1
pyrus_communis pycom06g01270
rosa_chinensis RchiOBHm_Chr7g0213461
rosa_laevigata RLG00000002802 RLG00000007782
rosa_multiflora Rmu_co8493477.1_g000002 Rmu_sc0004455.1_g000006 Rmu_sc0013012.1_g000011
rosa_roxburghii Rroxscaffold_3G00245860
rosa_rugosa Rorug07G0142400
rosa_samantha Rh4BG226300 Rh7AG274500 Rh7BG270000 Rh7CG293700 Rh7DG282000
rosa_wichuraiana Rw7G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 267
AccB7I CCANNNNNTGG 1 cut(s) 712
AccBSI CCGCTC 1 cut(s) 480
AccII CGCG 1 cut(s) 59
AciI CCGC 5 cut(s) 27, 59, 62, 413, 478
AclWI GGATC 3 cut(s) 423, 548, 681
AcsI RAATTY 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 662
AfiI CCNNNNNNNGG 3 cut(s) 118, 608, 712
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 2 cut(s) 118, 368
AjnI CCWGG 1 cut(s) 711
AluBI AGCT 3 cut(s) 52, 314, 457
AluI AGCT 3 cut(s) 52, 314, 457
Alw26I GTCTC 2 cut(s) 69, 347
AlwI GGATC 3 cut(s) 423, 548, 681
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 5 cut(s) 164, 227, 230, 263, 716
ApoI RAATTY 1 cut(s) 193
AspLEI GCGC 3 cut(s) 268, 298, 601
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 5 cut(s) 106, 202, 412, 605, 665
AsuNHI GCTAGC 1 cut(s) 461
AvaI CYCGRG 1 cut(s) 670
BbsI GAAGAC 2 cut(s) 318, 449
BbvI GCAGC 5 cut(s) 151, 214, 217, 250, 703
BccI CCATC 1 cut(s) 714
BciT130I CCWGG 1 cut(s) 713
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 2 cut(s) 69, 347
BfaI CTAG 2 cut(s) 402, 462
BfoI RGCGCY 2 cut(s) 299, 602
BglII AGATCT 1 cut(s) 520
BisI GCNGC 7 cut(s) 60, 63, 165, 228, 231, 264, 717
BlsI GCNGC 7 cut(s) 61, 64, 166, 229, 232, 265, 718
Bme1390I CCNGG 2 cut(s) 603, 713
BmeT110I CYCGRG 1 cut(s) 670
BmiI GGNNCC 2 cut(s) 106, 678
BmrFI CCNGG 2 cut(s) 603, 713
BmsI GCATC 1 cut(s) 362
BmtI GCTAGC 1 cut(s) 465
BoxI GACNNNNGTC 1 cut(s) 81
BpiI GAAGAC 2 cut(s) 318, 449
BpmI CTGGAG 3 cut(s) 127, 330, 609
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 603
BsaI GGTCTC 2 cut(s) 69, 347
BsaJI CCNNGG 2 cut(s) 627, 669
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 608, 712
Bse3DI GCAATG 1 cut(s) 472
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 2 cut(s) 627, 669
BseGI GGATG 4 cut(s) 244, 377, 637, 742
BseLI CCNNNNNNNGG 3 cut(s) 118, 608, 712
BseMI GCAATG 1 cut(s) 472
BseMII CTCAG 2 cut(s) 71, 501
BseRI GAGGAG 5 cut(s) 45, 48, 51, 59, 62
BseXI GCAGC 5 cut(s) 151, 214, 217, 250, 703
BseYI CCCAGC 1 cut(s) 173
BsgI GTGCAG 1 cut(s) 338
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 1 cut(s) 668
BsiHKCI CYCGRG 1 cut(s) 670
BsiSI CCGG 1 cut(s) 602
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 3 cut(s) 118, 608, 712
BsmAI GTCTC 2 cut(s) 69, 347
BsmFI GGGAC 1 cut(s) 288
BsnI GGCC 1 cut(s) 668
Bso31I GGTCTC 2 cut(s) 69, 347
BsoBI CYCGRG 1 cut(s) 670
Bsp143I GATC 4 cut(s) 415, 520, 553, 686
BspACI CCGC 5 cut(s) 27, 59, 62, 413, 478
BspANI GGCC 1 cut(s) 668
BspCNI CTCAG 2 cut(s) 72, 502
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 2 cut(s) 106, 678
BspOI GCTAGC 1 cut(s) 465
BspPI GGATC 3 cut(s) 423, 548, 681
BspTNI GGTCTC 2 cut(s) 69, 347
BsrBI CCGCTC 1 cut(s) 480
BsrDI GCAATG 1 cut(s) 472
BssECI CCNNGG 2 cut(s) 627, 669
BssMI GATC 4 cut(s) 415, 520, 553, 686
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 1 cut(s) 713
Bst6I CTCTTC 4 cut(s) 36, 124, 486, 638
BstC8I GCNNGC 2 cut(s) 459, 463
BstDEI CTNAG 2 cut(s) 80, 510
BstF5I GGATG 4 cut(s) 244, 377, 637, 742
BstFNI CGCG 1 cut(s) 59
BstH2I RGCGCY 2 cut(s) 299, 602
BstHHI GCGC 3 cut(s) 268, 298, 601
BstKTI GATC 4 cut(s) 418, 523, 556, 689
BstMAI GTCTC 2 cut(s) 69, 347
BstMBI GATC 4 cut(s) 415, 520, 553, 686
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 2 cut(s) 281, 626
BstPAI GACNNNNGTC 1 cut(s) 81
BstSCI CCNGG 2 cut(s) 601, 711
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 5 cut(s) 151, 214, 217, 250, 703
BstV2I GAAGAC 2 cut(s) 318, 449
BstX2I RGATCY 3 cut(s) 415, 520, 686
BstYI RGATCY 3 cut(s) 415, 520, 686
BsuRI GGCC 1 cut(s) 668
BtsCI GGATG 4 cut(s) 244, 377, 637, 742
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 2 cut(s) 350, 698
Cac8I GCNNGC 2 cut(s) 459, 463
CfoI GCGC 3 cut(s) 268, 298, 601
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviAII CATG 5 cut(s) 168, 244, 278, 377, 623
DdeI CTNAG 2 cut(s) 80, 510
DpnI GATC 4 cut(s) 417, 522, 555, 688
DpnII GATC 4 cut(s) 415, 520, 553, 686
Eam1104I CTCTTC 4 cut(s) 36, 124, 486, 638
EarI CTCTTC 4 cut(s) 36, 124, 486, 638
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 627
Eco147I AGGCCT 1 cut(s) 668
Eco31I GGTCTC 2 cut(s) 69, 347
Eco57I CTGAAG 1 cut(s) 662
Eco88I CYCGRG 1 cut(s) 670
EcoRII CCWGG 1 cut(s) 711
EcoT14I CCWWGG 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 627
FaeI CATG 5 cut(s) 171, 247, 281, 380, 626
FaiI YATR 8 cut(s) 169, 236, 245, 279, 378, 581, 624, 731
FaqI GGGAC 1 cut(s) 288
FatI CATG 5 cut(s) 167, 243, 277, 376, 622
Fnu4HI GCNGC 7 cut(s) 60, 63, 165, 228, 231, 264, 717
FokI GGATG 3 cut(s) 251, 384, 644
Fsp4HI GCNGC 7 cut(s) 60, 63, 165, 228, 231, 264, 717
FspBI CTAG 2 cut(s) 402, 462
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 3 cut(s) 267, 297, 600
GluI GCNGC 7 cut(s) 60, 63, 165, 228, 231, 264, 717
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 3 cut(s) 127, 330, 609
HaeII RGCGCY 2 cut(s) 299, 602
HaeIII GGCC 1 cut(s) 668
HapII CCGG 1 cut(s) 602
HhaI GCGC 3 cut(s) 268, 298, 601
Hin1II CATG 5 cut(s) 171, 247, 281, 380, 626
Hin6I GCGC 3 cut(s) 266, 296, 599
HinP1I GCGC 3 cut(s) 266, 296, 599
HinfI GANTC 3 cut(s) 82, 548, 656
HpaII CCGG 1 cut(s) 602
HphI GGTGA 5 cut(s) 106, 202, 412, 605, 665
Hpy188I TCNGA 4 cut(s) 511, 525, 553, 642
Hpy188III TCNNGA 5 cut(s) 72, 144, 309, 452, 588
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 4 cut(s) 10, 112, 241, 643
HpyCH4V TGCA 2 cut(s) 167, 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 2 cut(s) 80, 510
Hsp92II CATG 5 cut(s) 171, 247, 281, 380, 626
HspAI GCGC 3 cut(s) 266, 296, 599
Kzo9I GATC 4 cut(s) 415, 520, 553, 686
LmnI GCTCC 3 cut(s) 49, 110, 311
Lsp1109I GCAGC 5 cut(s) 151, 214, 217, 250, 703
LweI GCATC 1 cut(s) 362
MaeI CTAG 2 cut(s) 402, 462
MalI GATC 4 cut(s) 417, 522, 555, 688
MbiI CCGCTC 1 cut(s) 480
MboI GATC 4 cut(s) 415, 520, 553, 686
MboII GAAGA 9 cut(s) 53, 141, 297, 318, 454, 503, 557, 587, 655
MflI RGATCY 3 cut(s) 415, 520, 686
MluCI AATT 1 cut(s) 193
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 132
MseI TTAA 1 cut(s) 531
MspI CCGG 1 cut(s) 602
MspR9I CCNGG 2 cut(s) 603, 713
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 170
NciI CCSGG 1 cut(s) 603
NdeII GATC 4 cut(s) 415, 520, 553, 686
NheI GCTAGC 1 cut(s) 461
NlaIII CATG 5 cut(s) 171, 247, 281, 380, 626
NlaIV GGNNCC 2 cut(s) 106, 678
NsbI TGCGCA 1 cut(s) 267
NspI RCATGY 2 cut(s) 281, 626
PceI AGGCCT 1 cut(s) 668
PciI ACATGT 1 cut(s) 277
PfeI GAWTC 2 cut(s) 548, 656
PflMI CCANNNNNTGG 1 cut(s) 712
PkrI GCNGC 7 cut(s) 61, 64, 166, 229, 232, 265, 718
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
PscI ACATGT 1 cut(s) 277
PshAI GACNNNNGTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 2 cut(s) 106, 678
PsrI GAACNNNNNNTAC 2 cut(s) 273, 305
PsuI RGATCY 3 cut(s) 415, 520, 686
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 7 cut(s) 60, 63, 165, 228, 231, 264, 717
Sau3AI GATC 4 cut(s) 415, 520, 553, 686
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 603, 713
SfaNI GCATC 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 1 cut(s) 193
SseBI AGGCCT 1 cut(s) 668
SsiI CCGC 5 cut(s) 27, 59, 62, 413, 478
SspMI CTAG 2 cut(s) 402, 462
StuI AGGCCT 1 cut(s) 668
StyD4I CCNGG 2 cut(s) 601, 711
StyI CCWWGG 1 cut(s) 627
TaqI TCGA 1 cut(s) 10
TaqII GACCGA 1 cut(s) 307
TasI AATT 1 cut(s) 193
TauI GCSGC 2 cut(s) 62, 65
TfiI GAWTC 2 cut(s) 548, 656
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 2 cut(s) 357, 705
TseI GCWGC 5 cut(s) 164, 227, 230, 263, 716
TspDTI ATGAA 6 cut(s) 260, 486, 558, 596, 633, 648
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 2 cut(s) 357, 705
Van91I CCANNNNNTGG 1 cut(s) 712
XapI RAATTY 1 cut(s) 193
XceI RCATGY 2 cut(s) 281, 626
XspI CTAG 2 cut(s) 402, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.