Rroxscaffold_3G00245860

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012348
Physical Location & Seq
Reverse (-)
38951610 .. 38954344
2735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245860.1

Sequence Viewer

Length: 762 bp
ATGGGAAATCGACGGAAAGGTGCGAGTGGCGGAGGAGAAACAGACCTTCACGCGGCGGCGAGGTCCGGCGACCTGAGTGCAGTTCAGGTGATTGTCAGCTCCAACGCTTTGAAGGTCAATGGGAGAGATAAGCACTCCAGAACTCCACTACATTTAGCTGCGTGGGCTGGGCAAACGGAAGTGGTGAATTTTCTGTGTAAGAACAAGGCTGATGTTGGTGCTGCTGCTATGGATGACATGAAAGCAATACACTTTGCTGCACAAAAGGGACATTTAGAGGTTGTTCGGGCCCTACTTTCTTCTGGAGCTTCGGTCAAGTCTTCCACTCGCAAAGGTCTGACTCCGCTACACTTTGCTGTTCAAGGGTCCCATGTGGAACTTATCAAGTATTTGGCTAGGAAAGGTGCGGATCTCACTACAAAGACAAAAGCAGGGAAGACCCCTCTTGATCTTGCTAGCAATGATGAAATCCGCTCTTGTTTGGAAGAGTGTGAAAGGTCCTCTGAGAAAGGAGATCTGAATGGTAAACAGAAAGATGAAGAATCTGATCCAAAGACAACCCAGCAGGAAGATGTGAAATCTGGAGGTGAAGCTCCAGATTCAGTCAATGATGAACATGCCAAGGATGAAAGTCTGAAGAGGAAGGGTGATAATTCTGAGGAAGCCTCGGGGAACCTAAAAGAGCAAGAAAAGTCTAGGGCTAGTGGCCGGCATGGTGTATACTACTCTGACCTCTGTGAAGACTTCTTGTCCGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5

Protein Analysis

253

Amino Acids

27.03

Weight (kDa)

6.52

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 11 - 73 4.7e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 16 - 65 9.1e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 39 - 73 5.1e-06 Ankyrin repeats (many copies)
Ank PF00023 47 - 74 2.2e-06 Ankyrin repeat
Ank_2 PF12796 83 - 154 3.3e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 83 - 131 1.6e-12 Ankyrin repeats (many copies)
Ank_5 PF13857 103 - 152 7.6e-09 Ankyrin repeats (many copies)
Ank PF00023 112 - 142 4.7e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19150 AT4G19150
fragaria_vesca FvH4_5g24000
prunus_persica Prupe.5G019900_v2.0.a1
pyrus_communis pycom06g01270
rosa_chinensis RchiOBHm_Chr7g0213461
rosa_laevigata RLG00000002802 RLG00000007782
rosa_multiflora Rmu_co8493477.1_g000002 Rmu_sc0004455.1_g000006 Rmu_sc0013012.1_g000011
rosa_roxburghii Rroxscaffold_3G00245860
rosa_rugosa Rorug07G0142400
rosa_samantha Rh4BG226300 Rh7AG274500 Rh7BG270000 Rh7CG293700 Rh7DG282000
rosa_wichuraiana Rw7G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 474
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 53
AciI CCGC 6 cut(s) 30, 53, 56, 344, 407, 472
AclWI GGATC 2 cut(s) 417, 542
AcoI YGGCCR 1 cut(s) 706
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 656
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 2 cut(s) 112, 362
AhdI GACNNNNNGTC 1 cut(s) 748
AluBI AGCT 5 cut(s) 99, 158, 308, 593, 759
AluI AGCT 5 cut(s) 99, 158, 308, 593, 759
AlwI GGATC 2 cut(s) 417, 542
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 2 cut(s) 288, 706
ApaI GGGCCC 1 cut(s) 292
ApeKI GCWGC 5 cut(s) 158, 221, 224, 257, 756
ApoI RAATTY 1 cut(s) 187
AspS9I GGNCC 5 cut(s) 63, 288, 289, 366, 498
AsuHPI GGTGA 4 cut(s) 100, 196, 599, 659
AsuNHI GCTAGC 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 667
AvaII GGWCC 3 cut(s) 63, 366, 498
BaeGI GKGCMC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 292
BbsI GAAGAC 3 cut(s) 312, 443, 747
BbvI GCAGC 4 cut(s) 145, 208, 211, 244
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BfaI CTAG 4 cut(s) 396, 456, 696, 702
BglII AGATCT 1 cut(s) 514
BisI GCNGC 7 cut(s) 54, 57, 159, 222, 225, 258, 757
BlsI GCNGC 7 cut(s) 55, 58, 160, 223, 226, 259, 758
Bme18I GGWCC 3 cut(s) 63, 366, 498
BmeRI GACNNNNNGTC 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 667
BmgT120I GGNCC 5 cut(s) 63, 288, 289, 366, 498
BmiI GGNNCC 4 cut(s) 290, 367, 368, 674
BmtI GCTAGC 1 cut(s) 459
BpiI GAAGAC 3 cut(s) 312, 443, 747
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 4 cut(s) 121, 324, 579, 603
BsaJI CCNNGG 2 cut(s) 621, 666
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 708
Bse3DI GCAATG 1 cut(s) 466
BseDI CCNNGG 2 cut(s) 621, 666
BseGI GGATG 2 cut(s) 238, 631
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 65, 495, 648
BseRI GAGGAG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 292
BseXI GCAGC 4 cut(s) 145, 208, 211, 244
BseYI CCCAGC 2 cut(s) 167, 561
BsgI GTGCAG 2 cut(s) 99, 243
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 290, 708
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 3 cut(s) 66, 709, 753
BslFI GGGAC 2 cut(s) 282, 352
BslI CCNNNNNNNGG 1 cut(s) 52
BsmFI GGGAC 2 cut(s) 282, 352
BsnI GGCC 2 cut(s) 290, 708
BsoBI CYCGRG 1 cut(s) 667
Bsp120I GGGCCC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 4 cut(s) 409, 448, 514, 547
BspACI CCGC 6 cut(s) 30, 53, 56, 344, 407, 472
BspANI GGCC 2 cut(s) 290, 708
BspCNI CTCAG 3 cut(s) 66, 496, 649
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 4 cut(s) 290, 367, 368, 674
BspOI GCTAGC 1 cut(s) 459
BspPI GGATC 2 cut(s) 417, 542
BsrBI CCGCTC 1 cut(s) 474
BsrDI GCAATG 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 708
BssAI RCCGGY 1 cut(s) 708
BssECI CCNNGG 2 cut(s) 621, 666
BssMI GATC 4 cut(s) 409, 448, 514, 547
BssNAI GTATAC 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 621
Bst1107I GTATAC 1 cut(s) 721
Bst6I CTCTTC 2 cut(s) 480, 632
BstC8I GCNNGC 2 cut(s) 457, 710
BstDEI CTNAG 3 cut(s) 74, 504, 657
BstF5I GGATG 2 cut(s) 238, 631
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 4 cut(s) 412, 451, 517, 550
BstMBI GATC 4 cut(s) 409, 448, 514, 547
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 620
BstSLI GKGCMC 1 cut(s) 292
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 4 cut(s) 145, 208, 211, 244
BstV2I GAAGAC 3 cut(s) 312, 443, 747
BstX2I RGATCY 2 cut(s) 409, 514
BstYI RGATCY 2 cut(s) 409, 514
BstZ17I GTATAC 1 cut(s) 721
BsuRI GGCC 2 cut(s) 290, 708
BtsCI GGATG 2 cut(s) 238, 631
Cac8I GCNNGC 2 cut(s) 457, 710
Cfr10I RCCGGY 1 cut(s) 708
Cfr13I GGNCC 5 cut(s) 63, 288, 289, 366, 498
CviAII CATG 4 cut(s) 238, 371, 617, 713
DdeI CTNAG 3 cut(s) 74, 504, 657
DpnI GATC 4 cut(s) 411, 450, 516, 549
DpnII GATC 4 cut(s) 409, 448, 514, 547
DriI GACNNNNNGTC 1 cut(s) 748
EaeI YGGCCR 1 cut(s) 706
Eam1104I CTCTTC 2 cut(s) 480, 632
Eam1105I GACNNNNNGTC 1 cut(s) 748
EarI CTCTTC 2 cut(s) 480, 632
EciI GGCGGA 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 292
Eco47I GGWCC 3 cut(s) 63, 366, 498
Eco57I CTGAAG 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 667
EcoO109I RGGNCCY 3 cut(s) 289, 366, 498
EcoT14I CCWWGG 1 cut(s) 621
EcoT38I GRGCYC 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 4 cut(s) 241, 374, 620, 716
FaiI YATR 6 cut(s) 230, 239, 372, 618, 714, 721
FaqI GGGAC 2 cut(s) 282, 352
FatI CATG 4 cut(s) 237, 370, 616, 712
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 7 cut(s) 54, 57, 159, 222, 225, 258, 757
FokI GGATG 2 cut(s) 245, 638
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 7 cut(s) 54, 57, 159, 222, 225, 258, 757
FspBI CTAG 4 cut(s) 396, 456, 696, 702
GluI GCNGC 7 cut(s) 54, 57, 159, 222, 225, 258, 757
GsaI CCCAGC 2 cut(s) 171, 565
GsuI CTGGAG 4 cut(s) 121, 324, 579, 603
HaeIII GGCC 2 cut(s) 290, 708
HapII CCGG 3 cut(s) 66, 709, 753
Hin1II CATG 4 cut(s) 241, 374, 620, 716
HinfI GANTC 3 cut(s) 340, 542, 599
HpaII CCGG 3 cut(s) 66, 709, 753
HphI GGTGA 4 cut(s) 100, 196, 599, 659
Hpy166II GTNNAC 2 cut(s) 527, 721
Hpy188I TCNGA 7 cut(s) 339, 505, 519, 547, 636, 658, 730
Hpy188III TCNNGA 5 cut(s) 138, 303, 446, 582, 596
Hpy8I GTNNAC 2 cut(s) 527, 721
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 3 cut(s) 56, 106, 637
HpyCH4V TGCA 2 cut(s) 80, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 3 cut(s) 74, 504, 657
Hsp92II CATG 4 cut(s) 241, 374, 620, 716
KflI GGGWCCC 1 cut(s) 366
KroI GCCGGC 1 cut(s) 708
KroNI GCCGGC 1 cut(s) 710
Kzo9I GATC 4 cut(s) 409, 448, 514, 547
LmnI GCTCC 3 cut(s) 104, 305, 598
Lsp1109I GCAGC 4 cut(s) 145, 208, 211, 244
MaeI CTAG 4 cut(s) 396, 456, 696, 702
MalI GATC 4 cut(s) 411, 450, 516, 549
MbiI CCGCTC 1 cut(s) 474
MboI GATC 4 cut(s) 409, 448, 514, 547
MboII GAAGA 8 cut(s) 291, 312, 448, 497, 551, 581, 649, 752
MflI RGATCY 2 cut(s) 409, 514
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 2 cut(s) 187, 652
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 126
MroNI GCCGGC 1 cut(s) 708
MspA1I CMGCKG 1 cut(s) 759
MspI CCGG 3 cut(s) 66, 709, 753
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 1 cut(s) 164
NaeI GCCGGC 1 cut(s) 710
NdeII GATC 4 cut(s) 409, 448, 514, 547
NgoMIV GCCGGC 1 cut(s) 708
NheI GCTAGC 1 cut(s) 455
NlaIII CATG 4 cut(s) 241, 374, 620, 716
NlaIV GGNNCC 4 cut(s) 290, 367, 368, 674
NspI RCATGY 1 cut(s) 620
PdiI GCCGGC 1 cut(s) 710
PfeI GAWTC 2 cut(s) 542, 599
PkrI GCNGC 7 cut(s) 55, 58, 160, 223, 226, 259, 758
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
PpuMI RGGWCCY 2 cut(s) 366, 498
Psp5II RGGWCCY 2 cut(s) 366, 498
PspFI CCCAGC 2 cut(s) 167, 561
PspN4I GGNNCC 4 cut(s) 290, 367, 368, 674
PspOMI GGGCCC 1 cut(s) 288
PspPI GGNCC 5 cut(s) 63, 288, 289, 366, 498
PspPPI RGGWCCY 2 cut(s) 366, 498
PsuI RGATCY 2 cut(s) 409, 514
PvuII CAGCTG 1 cut(s) 759
SatI GCNGC 7 cut(s) 54, 57, 159, 222, 225, 258, 757
Sau3AI GATC 4 cut(s) 409, 448, 514, 547
Sau96I GGNCC 5 cut(s) 63, 288, 289, 366, 498
SchI GAGTC 1 cut(s) 334
SduI GDGCHC 1 cut(s) 292
SinI GGWCC 3 cut(s) 63, 366, 498
Sse9I AATT 2 cut(s) 187, 652
SsiI CCGC 6 cut(s) 30, 53, 56, 344, 407, 472
SspMI CTAG 4 cut(s) 396, 456, 696, 702
StyI CCWWGG 1 cut(s) 621
TaqI TCGA 1 cut(s) 10
TaqII GACCGA 1 cut(s) 301
TasI AATT 2 cut(s) 187, 652
TauI GCSGC 2 cut(s) 56, 59
TfiI GAWTC 2 cut(s) 542, 599
TseI GCWGC 5 cut(s) 158, 221, 224, 257, 756
TspDTI ATGAA 5 cut(s) 254, 480, 552, 627, 642
TspGWI ACGGA 2 cut(s) 28, 191
VpaK11BI GGWCC 3 cut(s) 63, 366, 498
XapI RAATTY 1 cut(s) 187
XceI RCATGY 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 4 cut(s) 396, 456, 696, 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.