Rorug07G0142400

Phosphoribosylglycinamide formyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012348
Physical Location & Seq
Reverse (-)
11252527 .. 11256779
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0142400.1

Sequence Viewer

Length: 660 bp
ATGATAGCAAAACGCAAAAGCCATCTCCTTCAACCACAGGCACTCCAACACAACACTCACTCCCTGCAACTCTCTCAGTTTATCTCAATGACTAGTACTTGTATTCAATTTCATTGCAAAGCTTCCAACTTTCTTGTTCCTTCACATTTTCTGGGTTCCTCTTCTTTCTTGCAGAGTCCCCAACCAAACCCACCACTAAGAAACAGAAACCCCAAGAGTCATACACAGAGAAGGATTAGCAATAGAAACATAAGAGGTGGGATTGTCTGTGGTGTGCTATTTCCAGTTGATCCATGGGCACCCAACATTGATTCACAGAGCATAGCTTCACAGCTCTTTGCAGCTTCTTTGTTTCCCTACCTTGGTTTCTTGTACTTTATCACCAAGTCCAAGACTGCCCCAAAGCTCACTCTTTTTGGGTTTTACTTCTTGCTAGCTTTTGTTGGGGCAACCATCCCTGCAGGAATTTATGCAAAGGTGAACTACGGCACTTCCTTGGCGAATGTTGATTGGTTACACGGTGGCGCTGAGTCACTTCTCACTCTAACCAACATATTCATTGTATTGGGGTTGAGAGAAGCTCTGAGGAAAGCTAAAGCAAACGAAGGAACGTCAGATCCTGTTCCAGGGTTGAAAGAGGAGAAGAAAACTTCTGTTTAG

Protein Analysis

219

Amino Acids

24.11

Weight (kDa)

10.02

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3593 PF12159 110 - 195 2.5e-39 Protein of unknown function (DUF3593)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19150 AT4G19150
fragaria_vesca FvH4_5g24000
prunus_persica Prupe.5G019900_v2.0.a1
pyrus_communis pycom06g01270
rosa_chinensis RchiOBHm_Chr7g0213461
rosa_laevigata RLG00000002802 RLG00000007782
rosa_multiflora Rmu_co8493477.1_g000002 Rmu_sc0004455.1_g000006 Rmu_sc0013012.1_g000011
rosa_roxburghii Rroxscaffold_3G00245860
rosa_rugosa Rorug07G0142400
rosa_samantha Rh4BG226300 Rh7AG274500 Rh7BG270000 Rh7CG293700 Rh7DG282000
rosa_wichuraiana Rw7G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 298
AclWI GGATC 2 cut(s) 284, 611
AcsI RAATTY 1 cut(s) 465
AfaI GTAC 2 cut(s) 97, 374
AfiI CCNNNNNNNGG 2 cut(s) 362, 626
AgsI TTSAA 3 cut(s) 32, 107, 634
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 1 cut(s) 625
AloI GAACNNNNNNTCC 2 cut(s) 601, 633
AluBI AGCT 8 cut(s) 122, 326, 334, 344, 406, 437, 581, 593
AluI AGCT 8 cut(s) 122, 326, 334, 344, 406, 437, 581, 593
AlwI GGATC 2 cut(s) 284, 611
AlwNI CAGNNNCTG 1 cut(s) 620
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 465
AspLEI GCGC 1 cut(s) 527
AsuHPI GGTGA 2 cut(s) 373, 490
AsuNHI GCTAGC 1 cut(s) 433
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 298
BbvI GCAGC 1 cut(s) 353
BccI CCATC 2 cut(s) 30, 461
BceAI ACGGC 1 cut(s) 502
BciT130I CCWGG 1 cut(s) 627
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 2 cut(s) 93, 434
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 528
BisI GCNGC 1 cut(s) 342
BlsI GCNGC 1 cut(s) 343
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 627
BmiI GGNNCC 2 cut(s) 157, 300
BmrFI CCNGG 1 cut(s) 627
BmtI GCTAGC 1 cut(s) 437
BplI GAGNNNNNCTC 2 cut(s) 565, 597
BsaJI CCNNGG 4 cut(s) 293, 361, 495, 626
BsaXI ACNNNNNCTCC 4 cut(s) 27, 44, 57, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 626
Bse1I ACTGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 112
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 4 cut(s) 293, 361, 495, 626
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 2 cut(s) 362, 626
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 3 cut(s) 89, 519, 575
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 653
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 1 cut(s) 353
BshNI GGYRCC 1 cut(s) 298
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 2 cut(s) 362, 626
BsmFI GGGAC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 2 cut(s) 289, 616
Bsp19I CCATGG 1 cut(s) 293
BspCNI CTCAG 3 cut(s) 88, 520, 576
BspLI GGNNCC 2 cut(s) 157, 300
BspMAI CTGCAG 1 cut(s) 463
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 2 cut(s) 284, 611
BspT107I GGYRCC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 112
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 4 cut(s) 293, 361, 495, 626
BssMI GATC 2 cut(s) 289, 616
BssT1I CCWWGG 3 cut(s) 293, 361, 495
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 1 cut(s) 521
Bst6I CTCTTC 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 4 cut(s) 75, 197, 528, 584
BstDSI CCRYGG 1 cut(s) 293
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 1 cut(s) 453
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 2 cut(s) 292, 619
BstMBI GATC 2 cut(s) 289, 616
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 625
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 301
BstV1I GCAGC 1 cut(s) 353
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 1 cut(s) 453
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 527
Csp6I GTAC 2 cut(s) 96, 373
CviAII CATG 1 cut(s) 294
CviJI RGCY 9 cut(s) 21, 122, 326, 334, 344, 406, 437, 581, 593
CviKI_1 RGCY 9 cut(s) 21, 122, 326, 334, 344, 406, 437, 581, 593
CviQI GTAC 2 cut(s) 96, 373
DdeI CTNAG 4 cut(s) 75, 197, 528, 584
DpnI GATC 2 cut(s) 291, 618
DpnII GATC 2 cut(s) 289, 616
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 293, 361, 495
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 3 cut(s) 293, 361, 495
ErhI CCWWGG 3 cut(s) 293, 361, 495
FaeI CATG 1 cut(s) 297
FaiI YATR 6 cut(s) 222, 251, 295, 323, 471, 554
FaqI GGGAC 1 cut(s) 162
FatI CATG 1 cut(s) 293
Fnu4HI GCNGC 1 cut(s) 342
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 2 cut(s) 93, 434
GlaI GCGC 1 cut(s) 526
GluI GCNGC 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 528
HhaI GCGC 1 cut(s) 527
Hin1II CATG 1 cut(s) 297
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 4 cut(s) 175, 217, 311, 530
HphI GGTGA 2 cut(s) 373, 490
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 2 cut(s) 585, 616
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 4 cut(s) 38, 150, 225, 599
HpyCH4III ACNGT 1 cut(s) 521
HpyCH4IV ACGT 1 cut(s) 611
HpyCH4V TGCA 6 cut(s) 67, 117, 172, 341, 461, 473
HpyF3I CTNAG 4 cut(s) 75, 197, 528, 584
HpySE526I ACGT 1 cut(s) 611
Hsp92II CATG 1 cut(s) 297
HspAI GCGC 1 cut(s) 525
Kzo9I GATC 2 cut(s) 289, 616
LpnPI CCDG 9 cut(s) 23, 77, 137, 297, 447, 471, 612, 633, 639
Lsp1109I GCAGC 1 cut(s) 353
MaeI CTAG 2 cut(s) 93, 434
MaeII ACGT 1 cut(s) 611
MaeIII GTNAC 2 cut(s) 513, 531
MalI GATC 2 cut(s) 291, 618
MboI GATC 2 cut(s) 289, 616
MboII GAAGA 2 cut(s) 153, 655
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 2 cut(s) 107, 465
MlyI GAGTC 3 cut(s) 184, 226, 539
MmeI TCCRAC 2 cut(s) 70, 150
MnlI CCTC 4 cut(s) 169, 248, 579, 631
MspR9I CCNGG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 627
NcoI CCATGG 1 cut(s) 293
NdeII GATC 2 cut(s) 289, 616
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 1 cut(s) 297
NlaIV GGNNCC 2 cut(s) 157, 300
NmuCI GTSAC 1 cut(s) 531
PfeI GAWTC 1 cut(s) 311
PkrI GCNGC 1 cut(s) 343
PleI GAGTC 3 cut(s) 183, 225, 538
PpsI GAGTC 3 cut(s) 183, 225, 538
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 2 cut(s) 157, 300
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 2 cut(s) 97, 374
RsaNI GTAC 2 cut(s) 96, 373
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 2 cut(s) 289, 616
SbfI CCTGCAGG 1 cut(s) 463
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 3 cut(s) 184, 226, 539
ScrFI CCNGG 1 cut(s) 627
SdaI CCTGCAGG 1 cut(s) 463
SduI GDGCHC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 92
Sse8387I CCTGCAGG 1 cut(s) 463
Sse9I AATT 2 cut(s) 107, 465
SspMI CTAG 2 cut(s) 93, 434
StyD4I CCNGG 1 cut(s) 625
StyI CCWWGG 3 cut(s) 293, 361, 495
TaaI ACNGT 1 cut(s) 521
TaiI ACGT 1 cut(s) 614
TasI AATT 2 cut(s) 107, 465
TatI WGTACW 2 cut(s) 95, 372
TfiI GAWTC 1 cut(s) 311
TseFI GTSAC 1 cut(s) 531
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 531
TspDTI ATGAA 2 cut(s) 101, 547
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 1 cut(s) 465
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
XspI CTAG 2 cut(s) 93, 434
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.