FvH4_5g27220

YacP-like NYN domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003444
Physical Location & Seq
Forward (+)
18505698 .. 18512409
6712 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27220.t6

Sequence Viewer

Length: 636 bp
ATGACGTTCCCGATGATCCGGAACTCTATCGTGATCCCACCACCACTCTCTACTAGTAATGCTCTCTCTGCCAGTACAAACCAGGGTTTTGAGAATGCAGTGCCTGTCTTGCTTGTTGATGGGTACAATGTGTGTGGCTATTGGGCCAAGCTCAAGAAACATTTTGTCAATGGGAGACTTGATATCGCACGCCAGAAGCTCATTGATGAGCTTGTCACTTTCAGCATGCTTAGAGAGGTGAAGGTGGTAGTTGTATTTGATGCCATGATGTCTGGACTACCCACGCATAAGGAAGATTTTGCCGGCGTTGATGTCGTTTTCTCAGGTGAAACTTGTGCTGATGCATGGATTGAAAAAGAGGTTGCAGGTTTAAGGGAGGATGGATGCCCTAAAGTTTGGGTTGTGACTTCTGATCATGTTCAGCAGCATGCAGCACATGGAGCAGGAGCCTTCATTTGGAGCAGCAAGGCACTGATTTCTGAGATTAAAGCATCACAGAAGGAGGTTGAGAGGATGCTTGAAGAACACAGGTCCACTTCTTTTCAAGGGAGGTTATTAAAGCACAATCTTGACTCAGAAGTTGTAGACGCTCTCAAGGATCTTAGAACAAAGTTATCTGAAACTGAACAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN_YacP

Protein Analysis

212

Amino Acids

23.45

Weight (kDa)

5.98

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NYN_YacP PF05991 37 - 202 5.2e-43 YacP-like NYN domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 585
AccIII TCCGGA 1 cut(s) 18
AclWI GGATC 3 cut(s) 10, 28, 606
AfaI GTAC 2 cut(s) 76, 125
AfiI CCNNNNNNNGG 1 cut(s) 456
AgsI TTSAA 3 cut(s) 353, 521, 545
AhlI ACTAGT 1 cut(s) 53
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 3 cut(s) 151, 199, 211
AluI AGCT 3 cut(s) 151, 199, 211
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 3 cut(s) 10, 28, 606
AlwNI CAGNNNCTG 1 cut(s) 104
Aor13HI TCCGGA 1 cut(s) 18
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 3 cut(s) 424, 431, 462
AspS9I GGNCC 2 cut(s) 144, 531
AsuHPI GGTGA 2 cut(s) 250, 338
AvaII GGWCC 1 cut(s) 531
BbvI GCAGC 3 cut(s) 436, 443, 474
BccI CCATC 2 cut(s) 113, 374
BciT130I CCWGG 1 cut(s) 83
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 1 cut(s) 169
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 356
BisI GCNGC 3 cut(s) 425, 432, 463
BlsI GCNGC 3 cut(s) 426, 433, 464
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 531
BmgT120I GGNCC 2 cut(s) 144, 531
BmiI GGNNCC 1 cut(s) 448
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 5 cut(s) 250, 331, 374, 500, 504
BpuEI CTTGAG 2 cut(s) 137, 578
BsaJI CCNNGG 1 cut(s) 82
BsaWI WCCGGW 1 cut(s) 18
Bsc4I CCNNNNNNNGG 1 cut(s) 456
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 72
BseAI TCCGGA 1 cut(s) 18
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 3 cut(s) 385, 389, 519
BseLI CCNNNNNNNGG 1 cut(s) 456
BseMII CTCAG 3 cut(s) 336, 471, 588
BseNI ACTGG 1 cut(s) 72
BseXI GCAGC 3 cut(s) 436, 443, 474
BshFI GGCC 1 cut(s) 146
BsiSI CCGG 2 cut(s) 19, 303
BslI CCNNNNNNNGG 1 cut(s) 456
BsmAI GTCTC 1 cut(s) 169
BsmI GAATGC 1 cut(s) 100
BsnI GGCC 1 cut(s) 146
Bsp13I TCCGGA 1 cut(s) 18
Bsp143I GATC 4 cut(s) 15, 33, 412, 598
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 3 cut(s) 335, 472, 587
BspEI TCCGGA 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 448
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 3 cut(s) 10, 28, 606
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 4 cut(s) 15, 33, 412, 598
Bst2UI CCWGG 1 cut(s) 83
BstC8I GCNNGC 4 cut(s) 190, 227, 304, 429
BstDEI CTNAG 5 cut(s) 230, 322, 480, 574, 602
BstF5I GGATG 3 cut(s) 385, 389, 519
BstKTI GATC 4 cut(s) 18, 36, 415, 601
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 4 cut(s) 15, 33, 412, 598
BstMWI GCNNNNNNNGC 3 cut(s) 68, 109, 440
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 2 cut(s) 229, 431
BstSCI CCNGG 1 cut(s) 81
BstV1I GCAGC 3 cut(s) 436, 443, 474
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 1 cut(s) 146
BtsCI GGATG 3 cut(s) 385, 389, 519
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 470
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 4 cut(s) 190, 227, 304, 429
CaiI CAGNNNCTG 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 2 cut(s) 144, 531
CseI GACGC 1 cut(s) 596
Csp6I GTAC 2 cut(s) 75, 124
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 6 cut(s) 226, 265, 345, 416, 428, 437
CviJI RGCY 6 cut(s) 138, 146, 151, 199, 211, 449
CviKI_1 RGCY 6 cut(s) 138, 146, 151, 199, 211, 449
CviQI GTAC 2 cut(s) 75, 124
DdeI CTNAG 5 cut(s) 230, 322, 480, 574, 602
DpnI GATC 4 cut(s) 17, 35, 414, 600
DpnII GATC 4 cut(s) 15, 33, 412, 598
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 81
EcoRV GATATC 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 346
FaeI CATG 6 cut(s) 229, 268, 348, 419, 431, 440
FaiI YATR 7 cut(s) 227, 266, 288, 346, 417, 429, 438
FatI CATG 6 cut(s) 225, 264, 344, 415, 427, 436
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 3 cut(s) 425, 432, 463
FokI GGATG 3 cut(s) 392, 396, 526
Fsp4HI GCNGC 3 cut(s) 425, 432, 463
FspBI CTAG 1 cut(s) 54
GluI GCNGC 3 cut(s) 425, 432, 463
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 19, 303
HgaI GACGC 1 cut(s) 596
Hin1II CATG 6 cut(s) 229, 268, 348, 419, 431, 440
HinfI GANTC 1 cut(s) 572
HpaII CCGG 2 cut(s) 19, 303
HphI GGTGA 2 cut(s) 250, 338
Hpy166II GTNNAC 2 cut(s) 534, 586
Hpy188I TCNGA 4 cut(s) 412, 481, 577, 619
Hpy188III TCNNGA 6 cut(s) 10, 19, 31, 154, 273, 569
Hpy8I GTNNAC 2 cut(s) 534, 586
HpyAV CCTTC 3 cut(s) 235, 460, 493
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 4 cut(s) 98, 344, 365, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 109, 440
HpyF3I CTNAG 5 cut(s) 230, 322, 480, 574, 602
HpySE526I ACGT 1 cut(s) 5
Hsp92II CATG 6 cut(s) 229, 268, 348, 419, 431, 440
Kpn2I TCCGGA 1 cut(s) 18
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 4 cut(s) 15, 33, 412, 598
LmnI GCTCC 3 cut(s) 440, 446, 459
Lsp1109I GCAGC 3 cut(s) 436, 443, 474
LweI GCATC 5 cut(s) 250, 331, 374, 500, 504
MaeI CTAG 1 cut(s) 54
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 214, 403
MalI GATC 4 cut(s) 17, 35, 414, 600
MboI GATC 4 cut(s) 15, 33, 412, 598
MboII GAAGA 2 cut(s) 305, 533
MflI RGATCY 1 cut(s) 598
MlyI GAGTC 1 cut(s) 566
MnlI CCTC 6 cut(s) 229, 352, 370, 496, 504, 543
Mph1103I ATGCAT 1 cut(s) 346
MroI TCCGGA 1 cut(s) 18
MroNI GCCGGC 1 cut(s) 302
MseI TTAA 3 cut(s) 371, 486, 557
MspI CCGG 2 cut(s) 19, 303
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 100
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 68, 109, 440
NaeI GCCGGC 1 cut(s) 304
NdeII GATC 4 cut(s) 15, 33, 412, 598
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 6 cut(s) 229, 268, 348, 419, 431, 440
NlaIV GGNNCC 1 cut(s) 448
NmuCI GTSAC 2 cut(s) 214, 403
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 2 cut(s) 229, 431
PaeI GCATGC 2 cut(s) 229, 431
PctI GAATGC 1 cut(s) 100
PdiI GCCGGC 1 cut(s) 304
PkrI GCNGC 3 cut(s) 426, 433, 464
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 2 cut(s) 144, 531
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 598
RsaI GTAC 2 cut(s) 76, 125
RsaNI GTAC 2 cut(s) 75, 124
SaqAI TTAA 3 cut(s) 371, 486, 557
SatI GCNGC 3 cut(s) 425, 432, 463
Sau3AI GATC 4 cut(s) 15, 33, 412, 598
Sau96I GGNCC 2 cut(s) 144, 531
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 1 cut(s) 83
SfaNI GCATC 5 cut(s) 250, 331, 374, 500, 504
SinI GGWCC 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 152, 593
SmoI CTYRAG 2 cut(s) 152, 593
SpeI ACTAGT 1 cut(s) 53
SphI GCATGC 2 cut(s) 229, 431
SspMI CTAG 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 81
TaiI ACGT 1 cut(s) 8
TatI WGTACW 1 cut(s) 74
Tru1I TTAA 3 cut(s) 371, 486, 557
Tru9I TTAA 3 cut(s) 371, 486, 557
TscAI CASTG 2 cut(s) 105, 477
TseFI GTSAC 2 cut(s) 214, 403
TseI GCWGC 3 cut(s) 424, 431, 462
Tsp45I GTSAC 2 cut(s) 214, 403
TspDTI ATGAA 1 cut(s) 442
TspRI CASTG 2 cut(s) 105, 477
VpaK11BI GGWCC 1 cut(s) 531
XceI RCATGY 2 cut(s) 229, 431
XmiI GTMKAC 1 cut(s) 585
XspI CTAG 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.