MD15G1445500.v1.1

YacP-like NYN domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003444
Physical Location & Seq
Reverse (-)
54858160 .. 54865693
7534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1445500.v1.1.491

Sequence Viewer

Length: 906 bp
ATGAAATCGGCGCCATGGTCGTCTTTAGTGTTTCAGTTGCCACGCCCACGAGGTTGCTATGGCTGTTCTTGTGCCCAGTTTCAGTTTGTTGTAATGGCCAAGGGCAAGAACAAGTCTCAACAATCATCGCCTCCTCCTCCTTTGGGGTCGGAGGCGCCAACTCCTCCGAGGATTACATCAAATGTGAGGCAGAATTTACAGCTTTTAAAGTTATGGAAGGAGTACCAAAACAGAAAGTCTAGCACACCTAAGCACTCTACTAGATACCGCCGAAAGAAGGTCCCCAAGGAACAGCTTCCCCAAGACCCTGACCTCTACCGCGATCCAACCACCACCCTCTATTATACTAACCAGGGTTGGGACATTGCTGTGCCCGTCTTGCTTGTTGATGGATATAACCTCTGCGGTTACTGGGCCAAGCTCAAGAAGCATTTTATGAATGGGAGACTTGATGTTGCTCGCCAAAAGTTAATCGATGAACTTGTTACTTTCAGCATGCTAAGAGAGGTGAAAGTGGTAGCTGTATTCGATGCCATGATGTCTGGACTCCCCACACACAAGGAAGATTTTAATGGTGTTGATGTAGTATACTCTGGTGAAACCTGTGCTGATGCGTGGATTGAGAAAGAGGTTGCAGCCTTAAGGGAGGATGGGTGCCCTAAAGTTTGGGTTGTGACCTCTGATCATATTCAGCAGCATGCAGCACATGGAGCAGGGGCTTTCATTTGGAGTTGCAAGGCATTGATTTCAGAGATTAAAGCATCACAGAAGGAGTTTGAAAGAATGCTTGAAGAACATAGGTCTACATCTTTTCAAGGGAGATTATTAAAACACAATCTTGATTCAGAAGTTGTTGACGCTCTGAAGGATCTTAGGAGGAAGTTGTCTGAAACTGAATCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN_YacP

Protein Analysis

302

Amino Acids

34.27

Weight (kDa)

9.19

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NYN_YacP PF05991 127 - 293 1.6e-41 YacP-like NYN domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 10, 154, 654
AccI GTMKAC 2 cut(s) 588, 803
AccII CGCG 1 cut(s) 321
AciI CCGC 3 cut(s) 268, 319, 405
AclWI GGATC 2 cut(s) 317, 876
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 884
AcyI GRCGYC 2 cut(s) 11, 155
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 3 cut(s) 143, 277, 358
AflII CTTAAG 1 cut(s) 640
AgsI TTSAA 3 cut(s) 779, 791, 815
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 4 cut(s) 202, 295, 421, 521
AluI AGCT 4 cut(s) 202, 295, 421, 521
Alw26I GTCTC 2 cut(s) 120, 439
AlwI GGATC 2 cut(s) 317, 876
AoxI GGCC 2 cut(s) 96, 414
ApeKI GCWGC 3 cut(s) 635, 694, 701
ApoI RAATTY 1 cut(s) 193
Asp700I GAANNNNTTC 1 cut(s) 294
AspLEI GCGC 2 cut(s) 13, 157
AspS9I GGNCC 2 cut(s) 280, 414
AsuHPI GGTGA 2 cut(s) 520, 608
AvaII GGWCC 1 cut(s) 280
BaeGI GKGCMC 3 cut(s) 76, 375, 659
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 3 cut(s) 10, 154, 654
BauI CACGAG 1 cut(s) 48
BbvI GCAGC 3 cut(s) 647, 706, 713
BccI CCATC 2 cut(s) 383, 644
BciT130I CCWGG 1 cut(s) 353
BclI TGATCA 1 cut(s) 682
BcoDI GTCTC 2 cut(s) 120, 439
BfaI CTAG 2 cut(s) 240, 261
BfoI RGCGCY 2 cut(s) 14, 158
BfrI CTTAAG 1 cut(s) 640
BisI GCNGC 3 cut(s) 636, 695, 702
BlsI GCNGC 3 cut(s) 637, 696, 703
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 280, 414
BmiI GGNNCC 4 cut(s) 12, 156, 282, 656
BmrFI CCNGG 1 cut(s) 353
BmrI ACTGGG 2 cut(s) 70, 421
BmsI GCATC 3 cut(s) 520, 601, 770
BmuI ACTGGG 2 cut(s) 70, 421
Bpu10I CCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 474
BsaHI GRCGYC 2 cut(s) 11, 155
BsaJI CCNNGG 5 cut(s) 14, 99, 167, 285, 352
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 277, 358
Bse1I ACTGG 2 cut(s) 76, 416
Bse3DI GCAATG 1 cut(s) 363
BseBI CCWGG 1 cut(s) 353
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 5 cut(s) 14, 99, 167, 285, 352
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 3 cut(s) 143, 277, 358
BseMI GCAATG 1 cut(s) 363
BseNI ACTGG 2 cut(s) 76, 416
BseRI GAGGAG 3 cut(s) 123, 126, 153
BseSI GKGCMC 3 cut(s) 76, 375, 659
BseXI GCAGC 3 cut(s) 647, 706, 713
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 2 cut(s) 98, 416
BshNI GGYRCC 3 cut(s) 10, 154, 654
BshVI ATCGAT 1 cut(s) 474
BslFI GGGAC 2 cut(s) 266, 374
BslI CCNNNNNNNGG 3 cut(s) 143, 277, 358
BsmAI GTCTC 2 cut(s) 120, 439
BsmFI GGGAC 2 cut(s) 266, 374
BsmI GAATGC 1 cut(s) 789
BsnI GGCC 2 cut(s) 98, 416
Bsp1286I GDGCHC 3 cut(s) 76, 375, 659
Bsp143I GATC 3 cut(s) 322, 682, 868
Bsp19I CCATGG 1 cut(s) 14
BspACI CCGC 3 cut(s) 268, 319, 405
BspANI GGCC 2 cut(s) 98, 416
BspDI ATCGAT 1 cut(s) 474
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 4 cut(s) 12, 156, 282, 656
BspPI GGATC 2 cut(s) 317, 876
BspT107I GGYRCC 3 cut(s) 10, 154, 654
BspTI CTTAAG 1 cut(s) 640
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 2 cut(s) 76, 416
BssECI CCNNGG 5 cut(s) 14, 99, 167, 285, 352
BssMI GATC 3 cut(s) 322, 682, 868
BssNAI GTATAC 1 cut(s) 589
BssNI GRCGYC 2 cut(s) 11, 155
BssSI CACGAG 1 cut(s) 48
BssT1I CCWWGG 3 cut(s) 14, 99, 285
Bst1107I GTATAC 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 353
BstACI GRCGYC 2 cut(s) 11, 155
BstAFI CTTAAG 1 cut(s) 640
BstC8I GCNNGC 3 cut(s) 460, 497, 699
BstDEI CTNAG 3 cut(s) 249, 500, 872
BstDSI CCRYGG 1 cut(s) 14
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 1 cut(s) 321
BstH2I RGCGCY 2 cut(s) 14, 158
BstHHI GCGC 2 cut(s) 13, 157
BstKTI GATC 3 cut(s) 325, 685, 871
BstMAI GTCTC 2 cut(s) 120, 439
BstMBI GATC 3 cut(s) 322, 682, 868
BstMWI GCNNNNNNNGC 3 cut(s) 379, 427, 710
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 2 cut(s) 499, 701
BstSCI CCNGG 1 cut(s) 351
BstSLI GKGCMC 3 cut(s) 76, 375, 659
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 3 cut(s) 647, 706, 713
BstX2I RGATCY 1 cut(s) 868
BstYI RGATCY 1 cut(s) 868
BstZ17I GTATAC 1 cut(s) 589
Bsu15I ATCGAT 1 cut(s) 474
BsuRI GGCC 2 cut(s) 98, 416
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 14
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 1 cut(s) 655
Cac8I GCNNGC 3 cut(s) 460, 497, 699
CfoI GCGC 2 cut(s) 13, 157
Cfr13I GGNCC 2 cut(s) 280, 414
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 1 cut(s) 866
Csp6I GTAC 1 cut(s) 223
CviAII CATG 5 cut(s) 15, 496, 535, 698, 707
CviJI RGCY 9 cut(s) 63, 98, 202, 295, 416, 421, 521, 638, 719
CviKI_1 RGCY 9 cut(s) 63, 98, 202, 295, 416, 421, 521, 638, 719
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 3 cut(s) 249, 500, 872
DinI GGCGCC 2 cut(s) 12, 156
DpnI GATC 3 cut(s) 324, 684, 870
DpnII GATC 3 cut(s) 322, 682, 868
DraI TTTAAA 1 cut(s) 207
EaeI YGGCCR 1 cut(s) 96
Eco130I CCWWGG 3 cut(s) 14, 99, 285
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 884
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 3 cut(s) 14, 99, 285
EgeI GGCGCC 2 cut(s) 12, 156
EheI GGCGCC 2 cut(s) 12, 156
ErhI CCWWGG 3 cut(s) 14, 99, 285
FaeI CATG 5 cut(s) 18, 499, 538, 701, 710
FaqI GGGAC 2 cut(s) 266, 374
FatI CATG 5 cut(s) 14, 495, 534, 697, 706
FbaI TGATCA 1 cut(s) 682
FblI GTMKAC 2 cut(s) 588, 803
Fnu4HI GCNGC 3 cut(s) 636, 695, 702
FokI GGATG 1 cut(s) 662
Fsp4HI GCNGC 3 cut(s) 636, 695, 702
FspBI CTAG 2 cut(s) 240, 261
GlaI GCGC 2 cut(s) 12, 156
GluI GCNGC 3 cut(s) 636, 695, 702
HaeII RGCGCY 2 cut(s) 14, 158
HaeIII GGCC 2 cut(s) 98, 416
HgaI GACGC 1 cut(s) 866
HhaI GCGC 2 cut(s) 13, 157
Hin1I GRCGYC 2 cut(s) 11, 155
Hin1II CATG 5 cut(s) 18, 499, 538, 701, 710
Hin6I GCGC 2 cut(s) 11, 155
HinP1I GCGC 2 cut(s) 11, 155
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HinfI GANTC 3 cut(s) 546, 842, 896
HphI GGTGA 2 cut(s) 520, 608
Hpy166II GTNNAC 3 cut(s) 589, 804, 856
Hpy188I TCNGA 7 cut(s) 151, 168, 682, 751, 847, 864, 889
Hpy188III TCNNGA 3 cut(s) 424, 543, 839
Hpy8I GTNNAC 3 cut(s) 589, 804, 856
HpyAV CCTTC 4 cut(s) 211, 271, 763, 859
HpyCH4V TGCA 3 cut(s) 635, 701, 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 379, 427, 710
HpyF3I CTNAG 3 cut(s) 249, 500, 872
Hsp92I GRCGYC 2 cut(s) 11, 155
Hsp92II CATG 5 cut(s) 18, 499, 538, 701, 710
HspAI GCGC 2 cut(s) 11, 155
KasI GGCGCC 2 cut(s) 10, 154
Ksp22I TGATCA 1 cut(s) 682
Kzo9I GATC 3 cut(s) 322, 682, 868
LmnI GCTCC 1 cut(s) 710
LpnPI CCDG 9 cut(s) 89, 321, 338, 365, 397, 528, 579, 616, 699
Lsp1109I GCAGC 3 cut(s) 647, 706, 713
LweI GCATC 3 cut(s) 520, 601, 770
MaeI CTAG 2 cut(s) 240, 261
MaeIII GTNAC 3 cut(s) 407, 484, 673
MalI GATC 3 cut(s) 324, 684, 870
MboI GATC 3 cut(s) 322, 682, 868
MboII GAAGA 2 cut(s) 575, 803
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 3 cut(s) 76, 375, 659
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 1 cut(s) 193
MluNI TGGCCA 1 cut(s) 98
Mly113I GGCGCC 2 cut(s) 11, 155
MlyI GAGTC 1 cut(s) 540
MmeI TCCRAC 2 cut(s) 129, 350
Mox20I TGGCCA 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 294
MscI TGGCCA 1 cut(s) 98
MseI TTAA 6 cut(s) 206, 470, 570, 641, 756, 827
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 640
MspR9I CCNGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 789
MvaI CCWGG 1 cut(s) 353
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 3 cut(s) 379, 427, 710
NarI GGCGCC 2 cut(s) 11, 155
NcoI CCATGG 1 cut(s) 14
NdeII GATC 3 cut(s) 322, 682, 868
NlaIII CATG 5 cut(s) 18, 499, 538, 701, 710
NlaIV GGNNCC 4 cut(s) 12, 156, 282, 656
NmuCI GTSAC 1 cut(s) 673
NspI RCATGY 2 cut(s) 499, 701
PaeI GCATGC 2 cut(s) 499, 701
PctI GAATGC 1 cut(s) 789
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 2 cut(s) 842, 896
PkrI GCNGC 3 cut(s) 637, 696, 703
PleI GAGTC 1 cut(s) 540
PluTI GGCGCC 2 cut(s) 14, 158
PpsI GAGTC 1 cut(s) 540
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 4 cut(s) 12, 156, 282, 656
PspPI GGNCC 2 cut(s) 280, 414
PspPPI RGGWCCY 1 cut(s) 280
PsuI RGATCY 1 cut(s) 868
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 6 cut(s) 206, 470, 570, 641, 756, 827
SatI GCNGC 3 cut(s) 636, 695, 702
Sau3AI GATC 3 cut(s) 322, 682, 868
Sau96I GGNCC 2 cut(s) 280, 414
SchI GAGTC 1 cut(s) 540
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 3 cut(s) 76, 375, 659
SfaNI GCATC 3 cut(s) 520, 601, 770
SfoI GGCGCC 2 cut(s) 12, 156
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 422, 640
SmoI CTYRAG 2 cut(s) 422, 640
SphI GCATGC 2 cut(s) 499, 701
Sse9I AATT 1 cut(s) 193
SsiI CCGC 3 cut(s) 268, 319, 405
SspDI GGCGCC 2 cut(s) 10, 154
SspMI CTAG 2 cut(s) 240, 261
StyD4I CCNGG 1 cut(s) 351
StyI CCWWGG 3 cut(s) 14, 99, 285
TaqI TCGA 3 cut(s) 474, 528, 899
TasI AATT 1 cut(s) 193
TfiI GAWTC 2 cut(s) 842, 896
Tru1I TTAA 6 cut(s) 206, 470, 570, 641, 756, 827
Tru9I TTAA 6 cut(s) 206, 470, 570, 641, 756, 827
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 3 cut(s) 635, 694, 701
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 4 cut(s) 17, 452, 492, 712
Vha464I CTTAAG 1 cut(s) 640
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 1 cut(s) 193
XceI RCATGY 2 cut(s) 499, 701
XmiI GTMKAC 2 cut(s) 588, 803
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 2 cut(s) 240, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.