Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0015112

Physical Location & Seq

Genomic Coordinates Forward (+)

19284223 .. 19291814

Gene Structure 7592 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g28050.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2952 bp

ATGGGCTCCCTAAAGAGAAAGCCGGAGGAAGATGGCTCCGGATCTCCCCCATCCAAGGTAGCCAAGTCGGAGACCGACGAACAGAGGGTGTGTGTGCACGACGTGTCGTACCCTGAGGGTTACGTTCCTCCACCTCCGCCTCCGCCTCCAGAGGGTTCCGAACCCGCCAAGAAATTCCCCTTCACTCTGGACCCTTTTCAATCCGAAGCCATCAATTGCATCGAAAAGGGCGAGTCCGTCATGGTGTCTGCTCACACTTCTGCTGGCAAGACTGTGGTGGCATCATATGCGATTGCGTTGTGCTTGCGAAACAAGCAACGCGTGATCTACACGGCGCCCATCAAAGCTCTCAGCAACCAAAAGTACCGTGAGTTCAAAGAGGAGTTTTCCGATGTCGGCTTGATGACCGGAGATGTCACCATTGAGCCCAACGCTTCGTGCTTGGTGATGACCACAGAGATCTGGCGAAGCATGCAGTACAAAGGATCCGAGGTGACGCGAGAGGTGGCTTGGATAATCTTTGATGAGGTGCATTACATGAGGGACCTCGAGAGAGGTGTGGTTTGGGAAGAGAGCATTGTTATGGCTCCTAAGAATTCTCGCTTTGTCTTCCTCTCTGCAACTGTGCCCAATGCTAAGGAGTTTGCTGATTGGCTCGCCAAGGTTCATCAACAGCCTTGCCACATTGTCTACACTGATTACAGGCCCACTCCTCTTCAGCATTATATTTTCCCCTCCGGAGGGGATGGTCTGTTCCTTGTAGTTGATGACAAGGGAAAGTTTCGTGAGGACAGCTTTCAGCAAGCTCTGAATGCACTTGTTCCTGCCTCTGACGCTGCTAAGAAGAAGGAAAATGGCAAGTGGCAGAAGGGTCTGGTCATCGGCAAGGCCGTTGAAGAAAGCGATATCTTCAAGATGGTGAAAATGATTATTCAGCGACAGTATGATCCTGTCATACTTTTCTGCTTTAGCAAAAGGGAATGTGAATCACTTGCGATGCAGATGGCAAAGTTGGACCTAAATGGGGACAGTGAGAAAGCAAACATTGAAACCATCTTCTGGAGTGCTATGGATATGCTTTCAGACGATGATAAGAAGTTACCCCAGGTTACCAGTATGTTTCCCCATTTAATCCGTGGAATAGGTGTTCATCATTCTGGTTTGATTCCCATTCTGAAGGAAGTGATTGAGATATTGTTTCAGGAAGGTCTTATCAAGTGTTTATTTGCGACAGAGACCTTCAGCATAGGGTTGAACATGCCTGCAAAAACTGTTGTGTTTACCAACGTCCGGAAATTTGATGGTAATAAGTTCAGATGGATAACCAGTGGTGAGTATATACAAATGAGTGGTCGTGCTGGTCGCCGTGGTATTGATAAACGTGGGGTATGCATACTTATGGTAGATGAGAAGCTGGAGCCATCTACTGCTAAAATGATGCTTAAAGGAAATGCCGATAGTTTGAATAGTGCCTTTCATTTAAGCTACAACATGCTTTTGAATCAATTGCGCTCCGAAGATGGTAATCCAGAAAATTTGCTCCGTAACTCATTCTATCAGTTCCAAGCTGATCGTGGCATTCCCAACCTGGAGAAACAAGCAAAGGAGCTTGAGCAAGAGAGGGATTCAATTATAATTGAAGAAGAAGATAGTGTAAAGAATTATTACAATCTAGTACAGCAATACAAGAGTTTAAAGCAAGATCTTCGTGAGATTGTGATTTCTCCCAAGTTCTGCTTACCATTTCTGAAACCTGGTAGGCTTGTGTCAATCCAATGCACCAAGAGTGATGAAGCTTCCACTTTTTCTACCAAGGATCATGTCACATGGGGAGTTATACTTAATTTTCAACTAGTGAAAACTGTTTCCGAAGACGATGCAGTTAAGAAACCAGAAGATTCAAACTACACAGTGGATGTTTTAACAAGATGTGTGGTCAGTACAAATGGAGTTGCAAAAAAAACTCTCAAAATTGTTCCTCTTAAAGAACCTGGAGAACCTGTCGTTGTTTCCGTTTCCATATCTCAGATTAATAGCATGAGTCGACTTTGTATGGTCATTCCAAATGATCTTTTATCAATACAAGCTCGAGAAAACACACTGAAGAAAGTTCTTGAAACTCTTTCCAGATTTGGAGACAAGATTCCTCTTCTAGATCCTGAAGAAGACATGAATATTCAAAGTAGTTCATACAAAAAAGTGGCTCGTAGGATTGAGGCTTTAGAGAACCTGTTTGACAGGCATGAAATTGCAAAAACACCACTAATTGAGCAAAAGCTGAAGGTTTATCACATGAAGCAGGAATTGGGAGCTAAGATAAAATCAATTAAGAGAACAATGCGCTCCTCTACTGCATTGGCTTTTAAAGATGAACTTAAGGCACGAAAACGGGTTCTTCGGAGGCTAGGATATATTACACGTGATGATGTTGTGGAGTTGAAGGGTAAGGTTGCTTGTGAAATCAGTAGTGCAGATGAGTTGACCCTCACAGAGCTCATGTTTAATGGTGTTTTTAAGGACATAAAGGTAGAAGAGTTGGTATCTCTTCTCTCATGTTTTGTTTGGCGGGAAAAACTAAAAGATGCTACAAAACCAAGAGAAGAACTTGACTTGCTCTTCTTGCAATTACAAGAAACTGCTCGGAGGGTGGCTGAAGTTCAGCTTGAGTGCAAGGTTGAAATTGACATTGATAGCTTTGTGAATTCTTTTCGGCCTGATATTATGGAGGCTGTATATGCTTGGGCAAAAGGGTCAAAGTTTTACGAGATAATGTCAGTTACAGGGGTTTTTGAGGGTAGTTTGATCAGGGCAATTAGGAGATTGGAGGAAGTTCTTCAGCAACTTATACATGCAGCCAATTCTATTGGAGAAACTGATCTTGAATCAAAATTTGAGGAGGCTGTTTTGAAAATCAAGAGAGACATCGTCTTTGCAGCTTCCCTATACTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0022613 GO:0031123 GO:0031125 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

984

Amino Acids

111.38

Weight (kDa)

6.38

Isoelectric Point (pI)

49.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	4 - 69	6.2e-08	DEAD/DEAH box helicase
MTR4-like_stalk	PF21408	366 - 428	7e-21	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	430 - 636	1.9e-49	Mtr4-like, beta-barrel domain
DSHCT	PF08148	663 - 833	3.6e-55	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015112)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59760
fragaria_vesca	FvH4_5g28050 FvH4_5g28050 FvH4_5g28050
malus_domestica	MD08G1247600.v1.1
prunus_persica	Prupe.6G113400_v2.0.a1
pyrus_communis	pycom08g21450
rosa_chinensis	RchiOBHm_Chr7g0220221
rosa_laevigata	RLG00000002249
rosa_multiflora	Rmu_sc0001136.1_g000019
rosa_roxburghii	Rroxscaffold_3G00239700
rosa_rugosa	Rorug07G0191200
rosa_samantha	Rh7AG333300 Rh7CG350900 Rh7DG329600
rosa_wichuraiana	Rw7G028110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1634, 2950
AccB1I	GGYRCC	1 cut(s)	334
AccB7I	CCANNNNNTGG	1 cut(s)	1059
AccI	GTMKAC	2 cut(s)	690, 2044
AccII	CGCG	2 cut(s)	321, 499
AccIII	TCCGGA	3 cut(s)	38, 737, 1290
AciI	CCGC	4 cut(s)	137, 143, 165, 2566
AclWI	GGATC	6 cut(s)	49, 480, 493, 941, 1824, 2150
AcsI	RAATTY	6 cut(s)	173, 595, 1295, 1534, 2701, 2888
AcuI	CTGAAG	8 cut(s)	701, 1196, 1225, 2123, 2181, 2300, 2673, 2819
AcvI	CACGTG	1 cut(s)	2420
AcyI	GRCGYC	1 cut(s)	335
AdeI	CACNNNGTG	1 cut(s)	103
AfaI	GTAC	5 cut(s)	110, 365, 479, 1677, 1942
AfiI	CCNNNNNNNGG	7 cut(s)	55, 152, 740, 741, 1024, 1059, 1290
AflII	CTTAAG	1 cut(s)	2375
AflIII	ACRYGT	3 cut(s)	102, 319, 2417
AhlI	ACTAGT	1 cut(s)	1852
AjiI	CACGTC	1 cut(s)	103
AjnI	CCWGG	4 cut(s)	1104, 1587, 1753, 1990
AjuI	GAANNNNNNNTTGG	2 cut(s)	2288, 2320
AloI	GAACNNNNNNTCC	4 cut(s)	737, 769, 1131, 1163
Alw21I	GWGCWC	2 cut(s)	99, 2496
Alw26I	GTCTC	4 cut(s)	65, 1229, 2130, 2913
Alw44I	GTGCAC	1 cut(s)	95
AlwI	GGATC	6 cut(s)	49, 480, 493, 941, 1824, 2150
Ama87I	CYCGRG	2 cut(s)	548, 2088
Aor13HI	TCCGGA	3 cut(s)	38, 737, 1290
AoxI	GGCC	3 cut(s)	704, 888, 2711
ApaLI	GTGCAC	1 cut(s)	95
ApeKI	GCWGC	3 cut(s)	836, 2852, 2933
ApoI	RAATTY	6 cut(s)	173, 595, 1295, 1534, 2701, 2888
ArsI	GACNNNNNNTTYG	4 cut(s)	1336, 1368, 2912, 2944
AseI	ATTAAT	1 cut(s)	2031
Asp700I	GAANNNNTTC	1 cut(s)	2832
AspLEI	GCGC	3 cut(s)	337, 1512, 2343
AspS9I	GGNCC	4 cut(s)	190, 544, 705, 1015
AsuHPI	GGTGA	5 cut(s)	409, 457, 505, 931, 1343
AvaI	CYCGRG	2 cut(s)	548, 2088
AvaII	GGWCC	3 cut(s)	190, 544, 1015
AxyI	CCTNAGG	1 cut(s)	114
BaeGI	GKGCMC	2 cut(s)	99, 630
BamHI	GGATCC	1 cut(s)	485
BanI	GGYRCC	1 cut(s)	334
BanII	GRGCYC	3 cut(s)	8, 429, 2496
BbrPI	CACGTG	1 cut(s)	2420
BbsI	GAAGAC	3 cut(s)	601, 1878, 2172
Bbv12I	GWGCWC	2 cut(s)	99, 2496
BbvI	GCAGC	3 cut(s)	823, 2864, 2945
BceAI	ACGGC	3 cut(s)	348, 875, 1350
BcgI	CGANNNNNNTGC	4 cut(s)	1688, 1722, 1859, 1893
BciT130I	CCWGG	4 cut(s)	1106, 1589, 1755, 1992
BclI	TGATCA	1 cut(s)	2802
BcoDI	GTCTC	4 cut(s)	65, 1229, 2130, 2913
BcuI	ACTAGT	1 cut(s)	1852
BfaI	CTAG	4 cut(s)	1673, 1853, 2153, 2405
BfoI	RGCGCY	1 cut(s)	338
BfrI	CTTAAG	1 cut(s)	2375
BglII	AGATCT	2 cut(s)	459, 1702
BisI	GCNGC	3 cut(s)	837, 2853, 2934
BlsI	GCNGC	3 cut(s)	838, 2854, 2935
Bme1390I	CCNGG	4 cut(s)	1106, 1589, 1755, 1992
Bme18I	GGWCC	3 cut(s)	190, 544, 1015
BmeT110I	CYCGRG	2 cut(s)	548, 2088
BmgBI	CACGTC	1 cut(s)	103
BmgT120I	GGNCC	4 cut(s)	190, 544, 705, 1015
BmiI	GGNNCC	9 cut(s)	7, 37, 157, 192, 336, 487, 545, 588, 1419
BmrFI	CCNGG	4 cut(s)	1106, 1589, 1755, 1992
BmsI	GCATC	6 cut(s)	228, 290, 987, 1428, 1867, 2572
BpiI	GAAGAC	3 cut(s)	601, 1878, 2172
BpmI	CTGGAG	5 cut(s)	132, 1081, 1436, 1610, 2013
Bpu10I	CCTNAGC	1 cut(s)	636
BpuEI	CTTGAG	2 cut(s)	1631, 2684
BsaAI	YACGTR	1 cut(s)	2420
BsaBI	GATNNNNATC	1 cut(s)	1524
BsaHI	GRCGYC	1 cut(s)	335
BsaI	GGTCTC	2 cut(s)	65, 1229
BsaJI	CCNNGG	7 cut(s)	54, 489, 660, 1104, 1135, 1366, 1812
BsaWI	WCCGGW	4 cut(s)	38, 407, 737, 1290
BsaXI	ACNNNNNCTCC	2 cut(s)	1986, 2016
Bsc4I	CCNNNNNNNGG	7 cut(s)	55, 152, 740, 741, 1024, 1059, 1290
Bse1I	ACTGG	2 cut(s)	1113, 1326
Bse21I	CCTNAGG	1 cut(s)	114
Bse8I	GATNNNNATC	1 cut(s)	1524
BseAI	TCCGGA	3 cut(s)	38, 737, 1290
BseBI	CCWGG	4 cut(s)	1106, 1589, 1755, 1992
BseDI	CCNNGG	7 cut(s)	54, 489, 660, 1104, 1135, 1366, 1812
BseGI	GGATG	3 cut(s)	50, 751, 1921
BseJI	GATNNNNATC	1 cut(s)	1524
BseLI	CCNNNNNNNGG	7 cut(s)	55, 152, 740, 741, 1024, 1059, 1290
BseMII	CTCAG	3 cut(s)	105, 364, 2039
BseNI	ACTGG	2 cut(s)	1113, 1326
BseRI	GAGGAG	4 cut(s)	395, 702, 2335, 2909
BseSI	GKGCMC	2 cut(s)	99, 630
BseXI	GCAGC	3 cut(s)	823, 2864, 2945
BsgI	GTGCAG	1 cut(s)	2490
Bsh1236I	CGCG	2 cut(s)	321, 499
BshFI	GGCC	3 cut(s)	706, 890, 2713
BshNI	GGYRCC	1 cut(s)	334
BsiHKAI	GWGCWC	2 cut(s)	99, 2496
BsiHKCI	CYCGRG	2 cut(s)	548, 2088
BsiSI	CCGG	5 cut(s)	23, 39, 408, 738, 1291
BslFI	GGGAC	2 cut(s)	557, 1040
BslI	CCNNNNNNNGG	7 cut(s)	55, 152, 740, 741, 1024, 1059, 1290
BsmAI	GTCTC	4 cut(s)	65, 1229, 2130, 2913
BsmFI	GGGAC	2 cut(s)	557, 1040
BsmI	GAATGC	2 cut(s)	817, 1578
BsnI	GGCC	3 cut(s)	706, 890, 2713
Bso31I	GGTCTC	2 cut(s)	65, 1229
BsoBI	CYCGRG	2 cut(s)	548, 2088
Bsp1286I	GDGCHC	5 cut(s)	8, 99, 429, 630, 2496
Bsp13I	TCCGGA	3 cut(s)	38, 737, 1290
BspACI	CCGC	4 cut(s)	137, 143, 165, 2566
BspANI	GGCC	3 cut(s)	706, 890, 2713
BspCNI	CTCAG	3 cut(s)	106, 363, 2038
BspEI	TCCGGA	3 cut(s)	38, 737, 1290
BspFNI	CGCG	2 cut(s)	321, 499
BspLI	GGNNCC	9 cut(s)	7, 37, 157, 192, 336, 487, 545, 588, 1419
BspPI	GGATC	6 cut(s)	49, 480, 493, 941, 1824, 2150
BspQI	GCTCTTC	1 cut(s)	2621
BspT107I	GGYRCC	1 cut(s)	334
BspTI	CTTAAG	1 cut(s)	2375
BspTNI	GGTCTC	2 cut(s)	65, 1229
BsrI	ACTGG	2 cut(s)	1113, 1326
BssECI	CCNNGG	7 cut(s)	54, 489, 660, 1104, 1135, 1366, 1812
BssNI	GRCGYC	1 cut(s)	335
BssT1I	CCWWGG	3 cut(s)	54, 660, 1812
Bst2UI	CCWGG	4 cut(s)	1106, 1589, 1755, 1992
Bst4CI	ACNGT	8 cut(s)	274, 368, 625, 942, 1031, 1273, 1864, 1912
Bst6I	CTCTTC	6 cut(s)	564, 720, 2154, 2526, 2550, 2621
BstACI	GRCGYC	1 cut(s)	335
BstAFI	CTTAAG	1 cut(s)	2375
BstAPI	GCANNNNNTGC	1 cut(s)	287
BstBAI	YACGTR	1 cut(s)	2420
BstC8I	GCNNGC	6 cut(s)	265, 305, 473, 657, 804, 1263
BstDEI	CTNAG	7 cut(s)	114, 350, 591, 636, 840, 2025, 2313
BstDSI	CCRYGG	2 cut(s)	1135, 1366
BstEII	GGTNACC	1 cut(s)	1108
BstF5I	GGATG	3 cut(s)	50, 751, 1921
BstFNI	CGCG	2 cut(s)	321, 499
BstH2I	RGCGCY	1 cut(s)	338
BstHHI	GCGC	3 cut(s)	337, 1512, 2343
BstMAI	GTCTC	4 cut(s)	65, 1229, 2130, 2913
BstMWI	GCNNNNNNNGC	7 cut(s)	287, 313, 472, 812, 833, 2620, 2735
BstNI	CCWGG	4 cut(s)	1106, 1589, 1755, 1992
BstNSI	RCATGY	4 cut(s)	475, 1261, 1495, 2852
BstPI	GGTNACC	1 cut(s)	1108
BstSCI	CCNGG	4 cut(s)	1104, 1587, 1753, 1990
BstSLI	GKGCMC	2 cut(s)	99, 630
BstUI	CGCG	2 cut(s)	321, 499
BstV1I	GCAGC	3 cut(s)	823, 2864, 2945
BstV2I	GAAGAC	3 cut(s)	601, 1878, 2172
BstX2I	RGATCY	5 cut(s)	41, 459, 485, 1702, 2155
BstYI	RGATCY	5 cut(s)	41, 459, 485, 1702, 2155
Bsu36I	CCTNAGG	1 cut(s)	114
BsuRI	GGCC	3 cut(s)	706, 890, 2713
BtgI	CCRYGG	2 cut(s)	1135, 1366
BtgZI	GCGATG	1 cut(s)	1010
BtrI	CACGTC	1 cut(s)	103
BtsCI	GGATG	3 cut(s)	50, 751, 1921
BtsIMutI	CAGTG	5 cut(s)	693, 1036, 1333, 1917, 2099
Cac8I	GCNNGC	6 cut(s)	265, 305, 473, 657, 804, 1263
CfoI	GCGC	3 cut(s)	337, 1512, 2343
Cfr13I	GGNCC	4 cut(s)	190, 544, 705, 1015
CseI	GACGC	2 cut(s)	505, 842
CsiI	ACCWGGT	1 cut(s)	1753
Csp6I	GTAC	5 cut(s)	109, 364, 478, 1676, 1941
CspCI	CAANNNNNGTGG	2 cut(s)	1914, 1949
CviQI	GTAC	5 cut(s)	109, 364, 478, 1676, 1941
DdeI	CTNAG	7 cut(s)	114, 350, 591, 636, 840, 2025, 2313
DinI	GGCGCC	1 cut(s)	336
DraI	TTTAAA	2 cut(s)	1695, 2365
DraIII	CACNNNGTG	1 cut(s)	103
Eam1104I	CTCTTC	6 cut(s)	564, 720, 2154, 2526, 2550, 2621
EarI	CTCTTC	6 cut(s)	564, 720, 2154, 2526, 2550, 2621
EciI	GGCGGA	2 cut(s)	126, 132
Ecl136II	GAGCTC	1 cut(s)	2494
Eco130I	CCWWGG	3 cut(s)	54, 660, 1812
Eco24I	GRGCYC	3 cut(s)	8, 429, 2496
Eco31I	GGTCTC	2 cut(s)	65, 1229
Eco32I	GATATC	1 cut(s)	907
Eco47I	GGWCC	3 cut(s)	190, 544, 1015
Eco53kI	GAGCTC	1 cut(s)	2494
Eco57I	CTGAAG	8 cut(s)	701, 1196, 1225, 2123, 2181, 2300, 2673, 2819
Eco72I	CACGTG	1 cut(s)	2420
Eco81I	CCTNAGG	1 cut(s)	114
Eco88I	CYCGRG	2 cut(s)	548, 2088
Eco91I	GGTNACC	1 cut(s)	1108
EcoICRI	GAGCTC	1 cut(s)	2494
EcoO109I	RGGNCCY	1 cut(s)	544
EcoO65I	GGTNACC	1 cut(s)	1108
EcoRI	GAATTC	2 cut(s)	595, 2701
EcoRII	CCWGG	4 cut(s)	1104, 1587, 1753, 1990
EcoRV	GATATC	1 cut(s)	907
EcoT14I	CCWWGG	3 cut(s)	54, 660, 1812
EcoT22I	ATGCAT	1 cut(s)	1394
EcoT38I	GRGCYC	3 cut(s)	8, 429, 2496
EgeI	GGCGCC	1 cut(s)	336
EheI	GGCGCC	1 cut(s)	336
ErhI	CCWWGG	3 cut(s)	54, 660, 1812
FalI	AAGNNNNNCTT	6 cut(s)	1721, 1753, 2358, 2390, 2646, 2678
FaqI	GGGAC	2 cut(s)	557, 1040
FauI	CCCGC	2 cut(s)	172, 2559
FauNDI	CATATG	1 cut(s)	286
FbaI	TGATCA	1 cut(s)	2802
FblI	GTMKAC	2 cut(s)	690, 2044
Fnu4HI	GCNGC	3 cut(s)	837, 2853, 2934
FokI	GGATG	3 cut(s)	37, 758, 1928
FriOI	GRGCYC	3 cut(s)	8, 429, 2496
Fsp4HI	GCNGC	3 cut(s)	837, 2853, 2934
FspBI	CTAG	4 cut(s)	1673, 1853, 2153, 2405
GlaI	GCGC	3 cut(s)	336, 1511, 2342
GluI	GCNGC	3 cut(s)	837, 2853, 2934
GsuI	CTGGAG	5 cut(s)	132, 1081, 1436, 1610, 2013
HaeII	RGCGCY	1 cut(s)	338
HaeIII	GGCC	3 cut(s)	706, 890, 2713
HapII	CCGG	5 cut(s)	23, 39, 408, 738, 1291
HgaI	GACGC	2 cut(s)	505, 842
HhaI	GCGC	3 cut(s)	337, 1512, 2343
Hin1I	GRCGYC	1 cut(s)	335
Hin6I	GCGC	3 cut(s)	335, 1510, 2341
HinP1I	GCGC	3 cut(s)	335, 1510, 2341
HincII	GTYRAC	2 cut(s)	2045, 2481
HindII	GTYRAC	2 cut(s)	2045, 2481
HindIII	AAGCTT	1 cut(s)	1794
HinfI	GANTC	9 cut(s)	233, 986, 1165, 1501, 1625, 1898, 2041, 2143, 2882
HpaII	CCGG	5 cut(s)	23, 39, 408, 738, 1291
HphI	GGTGA	5 cut(s)	409, 457, 505, 931, 1343
Hpy166II	GTNNAC	5 cut(s)	97, 691, 1281, 2045, 2481
Hpy8I	GTNNAC	5 cut(s)	97, 691, 1281, 2045, 2481
Hpy99I	CGWCG	2 cut(s)	80, 104
HpyAV	CCTTC	8 cut(s)	190, 841, 862, 1171, 1199, 1249, 2275, 2434
HpyCH4III	ACNGT	8 cut(s)	274, 368, 625, 942, 1031, 1273, 1864, 1912
HpyCH4IV	ACGT	5 cut(s)	102, 123, 1287, 1381, 2419
HpyF10VI	GCNNNNNNNGC	7 cut(s)	287, 313, 472, 812, 833, 2620, 2735
HpyF3I	CTNAG	7 cut(s)	114, 350, 591, 636, 840, 2025, 2313
HpySE526I	ACGT	5 cut(s)	102, 123, 1287, 1381, 2419
Hsp92I	GRCGYC	1 cut(s)	335
HspAI	GCGC	3 cut(s)	335, 1510, 2341
KasI	GGCGCC	1 cut(s)	334
Kpn2I	TCCGGA	3 cut(s)	38, 737, 1290
Ksp22I	TGATCA	1 cut(s)	2802
LguI	GCTCTTC	1 cut(s)	2621
LmnI	GCTCC	9 cut(s)	11, 41, 592, 1417, 1517, 1545, 1606, 2309, 2348
Lsp1109I	GCAGC	3 cut(s)	823, 2864, 2945
LweI	GCATC	6 cut(s)	228, 290, 987, 1428, 1867, 2572
MabI	ACCWGGT	1 cut(s)	1753
MaeI	CTAG	4 cut(s)	1673, 1853, 2153, 2405
MaeII	ACGT	5 cut(s)	102, 123, 1287, 1381, 2419
MaeIII	GTNAC	8 cut(s)	119, 415, 493, 1098, 1108, 1544, 1822, 2776
MfeI	CAATTG	2 cut(s)	214, 1505
MflI	RGATCY	5 cut(s)	41, 459, 485, 1702, 2155
MhlI	GDGCHC	5 cut(s)	8, 99, 429, 630, 2496
MluI	ACGCGT	1 cut(s)	319
Mly113I	GGCGCC	1 cut(s)	335
MlyI	GAGTC	2 cut(s)	242, 2050
MmeI	TCCRAC	2 cut(s)	48, 993
Mph1103I	ATGCAT	1 cut(s)	1394
MroI	TCCGGA	3 cut(s)	38, 737, 1290
MroXI	GAANNNNTTC	1 cut(s)	2832
MslI	CAYNNNNRTG	2 cut(s)	581, 1397
MspCI	CTTAAG	1 cut(s)	2375
MspI	CCGG	5 cut(s)	23, 39, 408, 738, 1291
MspR9I	CCNGG	4 cut(s)	1106, 1589, 1755, 1992
MunI	CAATTG	2 cut(s)	214, 1505
Mva1269I	GAATGC	2 cut(s)	817, 1578
MvaI	CCWGG	4 cut(s)	1106, 1589, 1755, 1992
MvnI	CGCG	2 cut(s)	321, 499
MwoI	GCNNNNNNNGC	7 cut(s)	287, 313, 472, 812, 833, 2620, 2735
NarI	GGCGCC	1 cut(s)	335
NdeI	CATATG	1 cut(s)	286
NlaIV	GGNNCC	9 cut(s)	7, 37, 157, 192, 336, 487, 545, 588, 1419
NmuCI	GTSAC	3 cut(s)	415, 493, 1822
NsiI	ATGCAT	1 cut(s)	1394
NspI	RCATGY	4 cut(s)	475, 1261, 1495, 2852
PaeI	GCATGC	1 cut(s)	475
PaeR7I	CTCGAG	2 cut(s)	548, 2088
PciSI	GCTCTTC	1 cut(s)	2621
PcsI	WCGNNNNNNNCGW	3 cut(s)	228, 888, 2010
PctI	GAATGC	2 cut(s)	817, 1578
PdmI	GAANNNNTTC	1 cut(s)	2832
PfeI	GAWTC	7 cut(s)	986, 1165, 1501, 1625, 1898, 2143, 2882
PflFI	GACNNNGTC	1 cut(s)	2924
PflMI	CCANNNNNTGG	1 cut(s)	1059
PkrI	GCNGC	3 cut(s)	838, 2854, 2935
PleI	GAGTC	2 cut(s)	241, 2049
PluTI	GGCGCC	1 cut(s)	338
PmaCI	CACGTG	1 cut(s)	2420
PmlI	CACGTG	1 cut(s)	2420
PpsI	GAGTC	2 cut(s)	241, 2049
Ppu21I	YACGTR	1 cut(s)	2420
PpuMI	RGGWCCY	1 cut(s)	544
PshBI	ATTAAT	1 cut(s)	2031
PsiI	TTATAA	2 cut(s)	1634, 2950
Psp124BI	GAGCTC	1 cut(s)	2496
Psp5II	RGGWCCY	1 cut(s)	544
Psp6I	CCWGG	4 cut(s)	1104, 1587, 1753, 1990
PspCI	CACGTG	1 cut(s)	2420
PspEI	GGTNACC	1 cut(s)	1108
PspGI	CCWGG	4 cut(s)	1104, 1587, 1753, 1990
PspN4I	GGNNCC	9 cut(s)	7, 37, 157, 192, 336, 487, 545, 588, 1419
PspPI	GGNCC	4 cut(s)	190, 544, 705, 1015
PspPPI	RGGWCCY	1 cut(s)	544
PsrI	GAACNNNNNNTAC	2 cut(s)	356, 388
PsuI	RGATCY	5 cut(s)	41, 459, 485, 1702, 2155
PsyI	GACNNNGTC	1 cut(s)	2924
RsaI	GTAC	5 cut(s)	110, 365, 479, 1677, 1942
RsaNI	GTAC	5 cut(s)	109, 364, 478, 1676, 1941
RseI	CAYNNNNRTG	2 cut(s)	581, 1397
SacI	GAGCTC	1 cut(s)	2496
SalI	GTCGAC	1 cut(s)	2043
SapI	GCTCTTC	1 cut(s)	2621
SatI	GCNGC	3 cut(s)	837, 2853, 2934
Sau96I	GGNCC	4 cut(s)	190, 544, 705, 1015
SchI	GAGTC	2 cut(s)	242, 2050
ScrFI	CCNGG	4 cut(s)	1106, 1589, 1755, 1992
SduI	GDGCHC	5 cut(s)	8, 99, 429, 630, 2496
SexAI	ACCWGGT	1 cut(s)	1753
SfaNI	GCATC	6 cut(s)	228, 290, 987, 1428, 1867, 2572
SfoI	GGCGCC	1 cut(s)	336
Sfr274I	CTCGAG	2 cut(s)	548, 2088
SinI	GGWCC	3 cut(s)	190, 544, 1015
SlaI	CTCGAG	2 cut(s)	548, 2088
SmiMI	CAYNNNNRTG	2 cut(s)	581, 1397
SmlI	CTYRAG	5 cut(s)	548, 1610, 2088, 2375, 2663
SmoI	CTYRAG	5 cut(s)	548, 1610, 2088, 2375, 2663
SpeI	ACTAGT	1 cut(s)	1852
SphI	GCATGC	1 cut(s)	475
SsiI	CCGC	4 cut(s)	137, 143, 165, 2566
SspDI	GGCGCC	1 cut(s)	334
SspI	AATATT	1 cut(s)	2176
SspMI	CTAG	4 cut(s)	1673, 1853, 2153, 2405
SstI	GAGCTC	1 cut(s)	2496
StyD4I	CCNGG	4 cut(s)	1104, 1587, 1753, 1990
StyI	CCWWGG	3 cut(s)	54, 660, 1812
TaaI	ACNGT	8 cut(s)	274, 368, 625, 942, 1031, 1273, 1864, 1912
TaiI	ACGT	5 cut(s)	105, 126, 1290, 1384, 2422
TaqI	TCGA	4 cut(s)	222, 549, 2044, 2089
TaqII	GACCGA	1 cut(s)	89
TatI	WGTACW	3 cut(s)	477, 1675, 1940
TfiI	GAWTC	7 cut(s)	986, 1165, 1501, 1625, 1898, 2143, 2882
TscAI	CASTG	5 cut(s)	700, 1036, 1333, 1917, 2106
TseFI	GTSAC	3 cut(s)	415, 493, 1822
TseI	GCWGC	3 cut(s)	836, 2852, 2933
Tsp45I	GTSAC	3 cut(s)	415, 493, 1822
TspDTI	ATGAA	9 cut(s)	656, 1139, 1466, 1806, 2178, 2186, 2259, 2309, 2385
TspGWI	ACGGA	4 cut(s)	226, 1124, 1532, 2002
TspRI	CASTG	5 cut(s)	700, 1036, 1333, 1917, 2106
Tth111I	GACNNNGTC	1 cut(s)	2924
Van91I	CCANNNNNTGG	1 cut(s)	1059
Vha464I	CTTAAG	1 cut(s)	2375
VneI	GTGCAC	1 cut(s)	95
VpaK11BI	GGWCC	3 cut(s)	190, 544, 1015
VspI	ATTAAT	1 cut(s)	2031
XapI	RAATTY	6 cut(s)	173, 595, 1295, 1534, 2701, 2888
XbaI	TCTAGA	1 cut(s)	2152
XceI	RCATGY	4 cut(s)	475, 1261, 1495, 2852
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1133, 1571
XhoI	CTCGAG	2 cut(s)	548, 2088
XmiI	GTMKAC	2 cut(s)	690, 2044
XmnI	GAANNNNTTC	1 cut(s)	2832
XspI	CTAG	4 cut(s)	1673, 1853, 2153, 2405
Zsp2I	ATGCAT	1 cut(s)	1394

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.