Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0015112

Physical Location & Seq

Genomic Coordinates Forward (+)

40007927 .. 40014135

Gene Structure 6209 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG329600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2976 bp

ATGGGATCCCTGAAGAGAAAATCGGAGGAAGATGACTCTGCAGCAGCTCCACCCAAACTAGCCAAGTCGGAGAACAAGAACACTCTTGAAATCGTCAACAATGAACAGATGGTCTGCGTGCACGACGTGTCGTACCCTGAGGGTTACGTTCCTCCGCCGCCGCCGCCGCCTTCTTCTTCCGAACCCGCCAAGAAATTCCCCTTCACTCTCGACCCTTTTCAGTCCGAAGCCATCAAATGCATTGAAAATGGGGAGTCCGTCATGGTGTCTGCTCACACATCTGCTGGAAAAACTGTTGTTGCGTCGTACGCGATTGCGTTGTGCTTGCGTAATAAGCAACGTGTGATATACACTGCACCCATCAAAGCTCTCAGCAACCAAAAGTACAGAGAGTTCAAAGAGGAGTTTTCGGATGTTGGGTTGATGACTGGAGATGTAACAATCGACCCCAACGCTTCGTGCTTGGTGATGACCACGGAAATCTGGCGAAGCATGCAGTACAAAGGATCAGAGGTGACGCGAGAGGTGGCGTGGATAATCTTTGATGAGGTGCATTACATGAGGGATCTTGAGAGAGGTGTGGTGTGGGAAGAGAGCATTGTTATGGCTCCAAAGAATGCTCGCTTTGTCTTCCTCTCAGCAACTGTGCCCAATGCTAAGGAATTTGCTGATTGGCTCGCAAAGGTTCATCGACAGCCTTGCCACATTGTGTATACGGATTATAGGCCCACTCCTCTTCAGCATTATATTTTCCCCTCCGGAGGGGATGGTCTGTACTTGGTAGTTGATGAAAAAGGAAAGTTTCGTGAGGACAGCTTTCAACAAGCTCTAAATGCGCTTGCTCCTGCCTCCGACGCTGCTAAGAAGAAGGAAAATGGCAAGTGGCAGAAGGGTCTGGTCATCGGCAAGGCCGCTGAAGAAAGCGATATCTTCAAGATGGTGAAAATGATAATTCAGCGTCAGTATGACCCTGTCATACTTTTCAGCTTTAGCAAAAGGGAATGTGAATTACTTGCAATGCAGATGGCAAAGTTGGACCTAAATGGGGTCAGTGAGAAAGCAAACATAGAAACCATCTTCTGGAGTGCTATGGATATGCTTTCAGACGACGATAAGAAGTTACCCCAGGTTACCACTATGTTACCCCTTTTAATGCGTGGAATAGGTGTGCATCATTCTGGTTTGATTCCCATTCTAAAGGAAGTGATTGAGATATTATTTCAGGAAGGTCTTATCAAGTGTTTATTTGCAACAGAGACCTTCAGCATAGGACTGAACATGCCTGCGAAAACTGTTGTATTTACCAATGTCCGGAAATTTGATGGTAATAAGTTCAGATGGATAACCAGTGGTGAGTATATACAAATGAGTGGTCGTGCTGGTCGCCGGGGTATTGATAAACGTGGGATATGCATACTTATGGTAGATGAGAAGCTAGAGCCATCTACTGCCAAAATGATGCTTAAAGGAAATGCTGATAGTTTGAACAGTGCCTTTCATTTAAGCTACAACATGCTTCTGAATCAATTGCGCTCTGAAGATGGTAATCCAGAAAATTTACTTCGTAACTCATTCTATCAGTTCCAAGCTGATCGTGCCATTCCCAATCTGGAGAAACAAACAAAGGAGCTTGAGCAAGAGAGGGATTCAATTATAATTGAAGAAGAAGATAGTGTAAGGAATTATTACAATCTAGTACAGCAATACAAGAGTTTAAAGCAGGATCTTCGTGAGATTGTAATTTCTCCAAAGTACTGCTTACCATTTCTGAAACCTGGTAGGCTTGTGTCAATCCAATGCACCAAGAGTGATGAAGCTTCCGCTTCTTTTTCTACCGAGGACCATGTCACATGGGGTGTTATACTTAATTTTCAACGAGTGAAAACTGTTTCTGAAGACGATGCAGTTAAGAGACCTGAAGATTCAAACTACACAGTGGATGTTTTGACTAGGTGTCTGGTTGGCACAAATGGAGTTGCAAAAAAAACACTCAAAATTGTTCCTTTGAAAGAACTGGGAGAACCTGTTGTTGTTTCGATTTCCATCTCTCAGATTAATAGCATGAGTCGACTTTGTATGGTCATACCAAATGATCTTTTGTCATTACAAGCTCGAGAAAACACACTGAAGAAAGTTCTTGAAACTCTTTCAAGATTTGGAGAGAAGATTCCTCTTTTAGATCCTGAAGAAGACATGAAGATTCAAAGTAGGTCGTACCAAAAAGTGGCTCGTAGGATTGAGGCTTTAGAGAACCTGTTTGACAGGCATGAAATTGCAAAGTCACCACTAATTGAGCAAAAGCTCAAGGTTTTTCACATAAAGCAGGAATTGGCAGCGAAGATCAAGTCAATTAAGAAAACAATGCGCTCTTCGACTGCGTTGGCTTTTAAAGATGAACTTAAGGCACGAAAACGTGTTCTTCGGAGGCTAGGATATGTTACTAGCGATGATGTTGTGCAGTTGAAGGGTAAGGTTGCTTGTGAAATCAGTAGTGCAGATGAGTTGACCCTCACAGAGCTCATGTTCAATGGCGTTTTCAAGGACATAAAGGTAGAAGAGATGGTATCTCTTCTCTCATGTTTTGTTTGGCGGGAAAAACTAAAAGATGCTACAAAACCAAGGGAAGAACTTGACCTGCTCTTCTTGCAATTACAAGAAACTGCTCGGAGGGTGGCTGAAGTTCAGCTTGAGTGCAAGGTTGAAATTGACATCGATAGCTTTGTGAGTTCATTTCGGCCTGATATTATGGAGGCTGCATATGCTTGGGCAAAAGGCTCAAAATTTTATGAGATAATGTCAGTTACAGGGGTTTTCGAGGGTAGCTTAATCAGGGCAATCAGGAGATTAGAGGAAGTTCTTCAGCAACTTATACACGCAGCCAATTCTATTGGAGAAACTGATCTTGAATCAAAATTTGAGGAGGCTGTTTCAAAAATCAAGAGAGACATTGTCTTTGCAGCTTCCCTATACTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0022613 GO:0031123 GO:0031125 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

991

Amino Acids

112.29

Weight (kDa)

6.58

Isoelectric Point (pI)

49.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	69 - 216	3.1e-09	Type III restriction enzyme, res subunit
DEAD	PF00270	72 - 221	6.9e-27	DEAD/DEAH box helicase
DEXQc_Suv3	PF22527	93 - 203	2e-07	DEXQ-box helicase domain of Suv3
MTR4-like_stalk	PF21408	518 - 580	1.7e-20	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	582 - 789	2.8e-48	Mtr4-like, beta-barrel domain
DSHCT	PF08148	816 - 986	6.8e-55	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015112)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59760
fragaria_vesca	FvH4_5g28050 FvH4_5g28050 FvH4_5g28050
malus_domestica	MD08G1247600.v1.1
prunus_persica	Prupe.6G113400_v2.0.a1
pyrus_communis	pycom08g21450
rosa_chinensis	RchiOBHm_Chr7g0220221
rosa_laevigata	RLG00000002249
rosa_multiflora	Rmu_sc0001136.1_g000019
rosa_roxburghii	Rroxscaffold_3G00239700
rosa_rugosa	Rorug07G0191200
rosa_samantha	Rh7AG333300 Rh7CG350900 Rh7DG329600
rosa_wichuraiana	Rw7G028110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1655
Acc36I	ACCTGC	1 cut(s)	2643
AccB7I	CCANNNNNTGG	2 cut(s)	1080, 2224
AccI	GTMKAC	2 cut(s)	713, 2068
AccII	CGCG	2 cut(s)	311, 520
AccIII	TCCGGA	2 cut(s)	758, 1311
AciI	CCGC	9 cut(s)	155, 158, 161, 164, 167, 186, 912, 1821, 2590
AclWI	GGATC	5 cut(s)	13, 514, 573, 1731, 2174
AcsI	RAATTY	6 cut(s)	194, 662, 1316, 1555, 2780, 2912
AdeI	CACNNNGTG	2 cut(s)	127, 709
AfaI	GTAC	8 cut(s)	134, 308, 386, 500, 776, 1698, 1754, 2216
AfiI	CCNNNNNNNGG	6 cut(s)	761, 762, 1045, 1080, 1311, 2224
AflII	CTTAAG	1 cut(s)	2399
AflIII	ACRYGT	3 cut(s)	126, 340, 2413
AhdI	GACNNNNNGTC	1 cut(s)	1953
AjiI	CACGTC	1 cut(s)	127
AjnI	CCWGG	2 cut(s)	1125, 1774
Alw21I	GWGCWC	2 cut(s)	123, 2520
Alw26I	GTCTC	3 cut(s)	1250, 1906, 2937
Alw44I	GTGCAC	1 cut(s)	119
AlwI	GGATC	5 cut(s)	13, 514, 573, 1731, 2174
AlwNI	CAGNNNCTG	1 cut(s)	644
Ama87I	CYCGRG	1 cut(s)	2112
Aor13HI	TCCGGA	2 cut(s)	758, 1311
AoxI	GGCC	3 cut(s)	725, 909, 2735
ApaLI	GTGCAC	1 cut(s)	119
ApeKI	GCWGC	7 cut(s)	41, 44, 857, 2333, 2753, 2876, 2957
ApoI	RAATTY	6 cut(s)	194, 662, 1316, 1555, 2780, 2912
ArsI	GACNNNNNNTTYG	6 cut(s)	1357, 1389, 2330, 2362, 2936, 2968
AseI	ATTAAT	1 cut(s)	2055
Asp700I	GAANNNNTTC	1 cut(s)	2856
AspLEI	GCGC	3 cut(s)	838, 1533, 2367
AspS9I	GGNCC	3 cut(s)	726, 1036, 1840
AsuC2I	CCSGG	1 cut(s)	1388
AsuHPI	GGTGA	5 cut(s)	478, 526, 952, 1364, 2274
AvaI	CYCGRG	1 cut(s)	2112
AvaII	GGWCC	2 cut(s)	1036, 1840
AxyI	CCTNAGG	1 cut(s)	138
BaeGI	GKGCMC	2 cut(s)	123, 651
BamHI	GGATCC	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	2520
BbsI	GAAGAC	3 cut(s)	622, 1902, 2196
Bbv12I	GWGCWC	2 cut(s)	123, 2520
BbvI	GCAGC	7 cut(s)	53, 56, 844, 2345, 2740, 2888, 2969
BcgI	CGANNNNNNTGC	4 cut(s)	681, 715, 1709, 1743
BciT130I	CCWGG	2 cut(s)	1127, 1776
BcnI	CCSGG	1 cut(s)	1388
BcoDI	GTCTC	3 cut(s)	1250, 1906, 2937
BfaI	CTAG	6 cut(s)	59, 1436, 1694, 1950, 2429, 2442
BfmI	CTRYAG	1 cut(s)	39
BfrI	CTTAAG	1 cut(s)	2399
BfuAI	ACCTGC	1 cut(s)	2643
BmcAI	AGTACT	1 cut(s)	1754
Bme1390I	CCNGG	3 cut(s)	1127, 1388, 1776
Bme18I	GGWCC	2 cut(s)	1036, 1840
BmeRI	GACNNNNNGTC	1 cut(s)	1953
BmeT110I	CYCGRG	1 cut(s)	2112
BmgBI	CACGTC	1 cut(s)	127
BmgT120I	GGNCC	3 cut(s)	726, 1036, 1840
BmiI	GGNNCC	2 cut(s)	7, 609
BmrFI	CCNGG	3 cut(s)	1127, 1388, 1776
BmrI	ACTGGG	1 cut(s)	2024
BmsI	GCATC	4 cut(s)	1180, 1449, 1891, 2596
BmuI	ACTGGG	1 cut(s)	2024
BpiI	GAAGAC	3 cut(s)	622, 1902, 2196
BpmI	CTGGAG	3 cut(s)	450, 1102, 1631
Bpu10I	CCTNAGC	1 cut(s)	657
BpuEI	CTTGAG	4 cut(s)	590, 1652, 2288, 2708
BpuMI	CCSGG	1 cut(s)	1388
Bsa29I	ATCGAT	1 cut(s)	2712
BsaBI	GATNNNNATC	2 cut(s)	1545, 2042
BsaI	GGTCTC	2 cut(s)	1250, 1906
BsaJI	CCNNGG	5 cut(s)	474, 1125, 1387, 1836, 2618
BsaWI	WCCGGW	2 cut(s)	758, 1311
BsaXI	ACNNNNNCTCC	2 cut(s)	2010, 2040
Bsc4I	CCNNNNNNNGG	6 cut(s)	761, 762, 1045, 1080, 1311, 2224
Bse1I	ACTGG	3 cut(s)	433, 1347, 2019
Bse21I	CCTNAGG	1 cut(s)	138
Bse3DI	GCAATG	1 cut(s)	1023
Bse8I	GATNNNNATC	2 cut(s)	1545, 2042
BseAI	TCCGGA	2 cut(s)	758, 1311
BseBI	CCWGG	2 cut(s)	1127, 1776
BseCI	ATCGAT	1 cut(s)	2712
BseDI	CCNNGG	5 cut(s)	474, 1125, 1387, 1836, 2618
BseGI	GGATG	3 cut(s)	418, 772, 1945
BseJI	GATNNNNATC	2 cut(s)	1545, 2042
BseLI	CCNNNNNNNGG	6 cut(s)	761, 762, 1045, 1080, 1311, 2224
BseMI	GCAATG	1 cut(s)	1023
BseMII	CTCAG	4 cut(s)	129, 385, 651, 2063
BseNI	ACTGG	3 cut(s)	433, 1347, 2019
BseRI	GAGGAG	3 cut(s)	416, 723, 2933
BseSI	GKGCMC	2 cut(s)	123, 651
BseXI	GCAGC	7 cut(s)	53, 56, 844, 2345, 2740, 2888, 2969
BsgI	GTGCAG	3 cut(s)	339, 2477, 2514
Bsh1236I	CGCG	2 cut(s)	311, 520
BshFI	GGCC	3 cut(s)	727, 911, 2737
BshVI	ATCGAT	1 cut(s)	2712
BsiHKAI	GWGCWC	2 cut(s)	123, 2520
BsiHKCI	CYCGRG	1 cut(s)	2112
BsiSI	CCGG	3 cut(s)	759, 1312, 1387
BsiWI	CGTACG	1 cut(s)	306
BslI	CCNNNNNNNGG	6 cut(s)	761, 762, 1045, 1080, 1311, 2224
BsmAI	GTCTC	3 cut(s)	1250, 1906, 2937
BsmI	GAATGC	1 cut(s)	622
BsnI	GGCC	3 cut(s)	727, 911, 2737
Bso31I	GGTCTC	2 cut(s)	1250, 1906
BsoBI	CYCGRG	1 cut(s)	2112
Bsp1286I	GDGCHC	3 cut(s)	123, 651, 2520
Bsp13I	TCCGGA	2 cut(s)	758, 1311
Bsp143I	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
BspACI	CCGC	9 cut(s)	155, 158, 161, 164, 167, 186, 912, 1821, 2590
BspANI	GGCC	3 cut(s)	727, 911, 2737
BspCNI	CTCAG	4 cut(s)	130, 384, 650, 2062
BspDI	ATCGAT	1 cut(s)	2712
BspEI	TCCGGA	2 cut(s)	758, 1311
BspFNI	CGCG	2 cut(s)	311, 520
BspLI	GGNNCC	2 cut(s)	7, 609
BspMAI	CTGCAG	1 cut(s)	43
BspMI	ACCTGC	1 cut(s)	2643
BspPI	GGATC	5 cut(s)	13, 514, 573, 1731, 2174
BspQI	GCTCTTC	2 cut(s)	2374, 2645
BspTI	CTTAAG	1 cut(s)	2399
BspTNI	GGTCTC	2 cut(s)	1250, 1906
BsrDI	GCAATG	1 cut(s)	1023
BsrI	ACTGG	3 cut(s)	433, 1347, 2019
BssECI	CCNNGG	5 cut(s)	474, 1125, 1387, 1836, 2618
BssMI	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
BssNAI	GTATAC	1 cut(s)	714
BssT1I	CCWWGG	1 cut(s)	2618
Bst1107I	GTATAC	1 cut(s)	714
Bst2UI	CCWGG	2 cut(s)	1127, 1776
Bst4CI	ACNGT	6 cut(s)	295, 646, 1294, 1490, 1888, 1936
Bst6I	CTCTTC	7 cut(s)	8, 585, 741, 2374, 2550, 2574, 2645
BstAFI	CTTAAG	1 cut(s)	2399
BstC8I	GCNNGC	7 cut(s)	119, 326, 494, 622, 678, 840, 1284
BstDEI	CTNAG	6 cut(s)	138, 371, 637, 657, 861, 2049
BstDSI	CCRYGG	1 cut(s)	474
BstEII	GGTNACC	1 cut(s)	1129
BstF5I	GGATG	3 cut(s)	418, 772, 1945
BstFNI	CGCG	2 cut(s)	311, 520
BstHHI	GCGC	3 cut(s)	838, 1533, 2367
BstKTI	GATC	9 cut(s)	8, 509, 568, 1594, 1726, 2095, 2182, 2343, 2902
BstMAI	GTCTC	3 cut(s)	1250, 1906, 2937
BstMBI	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
BstNI	CCWGG	2 cut(s)	1127, 1776
BstNSI	RCATGY	3 cut(s)	496, 1282, 1516
BstPI	GGTNACC	1 cut(s)	1129
BstSCI	CCNGG	3 cut(s)	1125, 1386, 1774
BstSFI	CTRYAG	1 cut(s)	39
BstSLI	GKGCMC	2 cut(s)	123, 651
BstUI	CGCG	2 cut(s)	311, 520
BstV1I	GCAGC	7 cut(s)	53, 56, 844, 2345, 2740, 2888, 2969
BstV2I	GAAGAC	3 cut(s)	622, 1902, 2196
BstX2I	RGATCY	4 cut(s)	5, 565, 1723, 2179
BstYI	RGATCY	4 cut(s)	5, 565, 1723, 2179
BstZ17I	GTATAC	1 cut(s)	714
Bsu15I	ATCGAT	1 cut(s)	2712
Bsu36I	CCTNAGG	1 cut(s)	138
BsuRI	GGCC	3 cut(s)	727, 911, 2737
BsuTUI	ATCGAT	1 cut(s)	2712
BtgI	CCRYGG	1 cut(s)	474
BtgZI	GCGATG	1 cut(s)	2460
BtrI	CACGTC	1 cut(s)	127
BtsCI	GGATG	3 cut(s)	418, 772, 1945
BtsI	GCAGTG	1 cut(s)	351
BtsIMutI	CAGTG	6 cut(s)	351, 1057, 1354, 1495, 1941, 2123
BveI	ACCTGC	1 cut(s)	2643
Cac8I	GCNNGC	7 cut(s)	119, 326, 494, 622, 678, 840, 1284
CaiI	CAGNNNCTG	1 cut(s)	644
CfoI	GCGC	3 cut(s)	838, 1533, 2367
Cfr13I	GGNCC	3 cut(s)	726, 1036, 1840
ClaI	ATCGAT	1 cut(s)	2712
CseI	GACGC	4 cut(s)	291, 526, 863, 947
CsiI	ACCWGGT	1 cut(s)	1774
Csp6I	GTAC	8 cut(s)	133, 307, 385, 499, 775, 1697, 1753, 2215
CviQI	GTAC	8 cut(s)	133, 307, 385, 499, 775, 1697, 1753, 2215
DdeI	CTNAG	6 cut(s)	138, 371, 637, 657, 861, 2049
DpnI	GATC	9 cut(s)	7, 508, 567, 1593, 1725, 2094, 2181, 2342, 2901
DpnII	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
DraI	TTTAAA	2 cut(s)	1716, 2389
DraIII	CACNNNGTG	2 cut(s)	127, 709
DriI	GACNNNNNGTC	1 cut(s)	1953
Eam1104I	CTCTTC	7 cut(s)	8, 585, 741, 2374, 2550, 2574, 2645
Eam1105I	GACNNNNNGTC	1 cut(s)	1953
EarI	CTCTTC	7 cut(s)	8, 585, 741, 2374, 2550, 2574, 2645
EciI	GGCGGA	1 cut(s)	144
Ecl136II	GAGCTC	1 cut(s)	2518
Eco130I	CCWWGG	1 cut(s)	2618
Eco24I	GRGCYC	1 cut(s)	2520
Eco31I	GGTCTC	2 cut(s)	1250, 1906
Eco32I	GATATC	1 cut(s)	928
Eco47I	GGWCC	2 cut(s)	1036, 1840
Eco53kI	GAGCTC	1 cut(s)	2518
Eco81I	CCTNAGG	1 cut(s)	138
Eco88I	CYCGRG	1 cut(s)	2112
Eco91I	GGTNACC	1 cut(s)	1129
EcoICRI	GAGCTC	1 cut(s)	2518
EcoO65I	GGTNACC	1 cut(s)	1129
EcoRII	CCWGG	2 cut(s)	1125, 1774
EcoRV	GATATC	1 cut(s)	928
EcoT14I	CCWWGG	1 cut(s)	2618
EcoT22I	ATGCAT	2 cut(s)	242, 1415
EcoT38I	GRGCYC	1 cut(s)	2520
ErhI	CCWWGG	1 cut(s)	2618
FalI	AAGNNNNNCTT	6 cut(s)	1742, 1774, 2382, 2414, 2670, 2702
FauI	CCCGC	2 cut(s)	193, 2583
FauNDI	CATATG	1 cut(s)	2758
FblI	GTMKAC	2 cut(s)	713, 2068
FokI	GGATG	3 cut(s)	425, 779, 1952
FriOI	GRGCYC	1 cut(s)	2520
FspBI	CTAG	6 cut(s)	59, 1436, 1694, 1950, 2429, 2442
GlaI	GCGC	3 cut(s)	837, 1532, 2366
GsuI	CTGGAG	3 cut(s)	450, 1102, 1631
HaeIII	GGCC	3 cut(s)	727, 911, 2737
HapII	CCGG	3 cut(s)	759, 1312, 1387
HgaI	GACGC	4 cut(s)	291, 526, 863, 947
HhaI	GCGC	3 cut(s)	838, 1533, 2367
Hin6I	GCGC	3 cut(s)	836, 1531, 2365
HinP1I	GCGC	3 cut(s)	836, 1531, 2365
HincII	GTYRAC	3 cut(s)	97, 2069, 2505
HindII	GTYRAC	3 cut(s)	97, 2069, 2505
HindIII	AAGCTT	1 cut(s)	1815
HpaII	CCGG	3 cut(s)	759, 1312, 1387
HphI	GGTGA	5 cut(s)	478, 526, 952, 1364, 2274
Hpy166II	GTNNAC	5 cut(s)	97, 121, 714, 2069, 2505
Hpy8I	GTNNAC	5 cut(s)	97, 121, 714, 2069, 2505
Hpy99I	CGWCG	4 cut(s)	128, 307, 857, 1112
HpyAV	CCTTC	7 cut(s)	180, 211, 862, 883, 1220, 1270, 2458
HpyCH4III	ACNGT	6 cut(s)	295, 646, 1294, 1490, 1888, 1936
HpyCH4IV	ACGT	5 cut(s)	126, 147, 340, 1402, 2413
HpyF3I	CTNAG	6 cut(s)	138, 371, 637, 657, 861, 2049
HpySE526I	ACGT	5 cut(s)	126, 147, 340, 1402, 2413
HspAI	GCGC	3 cut(s)	836, 1531, 2365
Kpn2I	TCCGGA	2 cut(s)	758, 1311
Kzo9I	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
LguI	GCTCTTC	2 cut(s)	2374, 2645
LmnI	GCTCC	4 cut(s)	52, 613, 847, 1627
Lsp1109I	GCAGC	7 cut(s)	53, 56, 844, 2345, 2740, 2888, 2969
LweI	GCATC	4 cut(s)	1180, 1449, 1891, 2596
MabI	ACCWGGT	1 cut(s)	1774
MaeI	CTAG	6 cut(s)	59, 1436, 1694, 1950, 2429, 2442
MaeII	ACGT	5 cut(s)	126, 147, 340, 1402, 2413
MalI	GATC	9 cut(s)	7, 508, 567, 1593, 1725, 2094, 2181, 2342, 2901
MboI	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
MfeI	CAATTG	1 cut(s)	1526
MflI	RGATCY	4 cut(s)	5, 565, 1723, 2179
MhlI	GDGCHC	3 cut(s)	123, 651, 2520
MlyI	GAGTC	3 cut(s)	29, 263, 2074
MmeI	TCCRAC	3 cut(s)	48, 876, 1014
Mph1103I	ATGCAT	2 cut(s)	242, 1415
MroI	TCCGGA	2 cut(s)	758, 1311
MroXI	GAANNNNTTC	1 cut(s)	2856
MslI	CAYNNNNRTG	2 cut(s)	602, 1418
MspA1I	CMGCKG	1 cut(s)	914
MspCI	CTTAAG	1 cut(s)	2399
MspI	CCGG	3 cut(s)	759, 1312, 1387
MspR9I	CCNGG	3 cut(s)	1127, 1388, 1776
MunI	CAATTG	1 cut(s)	1526
Mva1269I	GAATGC	1 cut(s)	622
MvaI	CCWGG	2 cut(s)	1127, 1776
MvnI	CGCG	2 cut(s)	311, 520
NciI	CCSGG	1 cut(s)	1388
NdeI	CATATG	1 cut(s)	2758
NdeII	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
NlaIV	GGNNCC	2 cut(s)	7, 609
NmuCI	GTSAC	3 cut(s)	514, 1846, 2280
NsiI	ATGCAT	2 cut(s)	242, 1415
NspI	RCATGY	3 cut(s)	496, 1282, 1516
PaeI	GCATGC	1 cut(s)	496
PaeR7I	CTCGAG	1 cut(s)	2112
PciSI	GCTCTTC	2 cut(s)	2374, 2645
PctI	GAATGC	1 cut(s)	622
PdmI	GAANNNNTTC	1 cut(s)	2856
PfeI	GAWTC	7 cut(s)	1186, 1522, 1646, 1922, 2167, 2200, 2906
Pfl23II	CGTACG	1 cut(s)	306
PflFI	GACNNNGTC	3 cut(s)	971, 1844, 2948
PflMI	CCANNNNNTGG	2 cut(s)	1080, 2224
PleI	GAGTC	3 cut(s)	29, 262, 2073
PpsI	GAGTC	3 cut(s)	29, 262, 2073
PshBI	ATTAAT	1 cut(s)	2055
PsiI	TTATAA	1 cut(s)	1655
Psp124BI	GAGCTC	1 cut(s)	2520
Psp6I	CCWGG	2 cut(s)	1125, 1774
PspEI	GGTNACC	1 cut(s)	1129
PspGI	CCWGG	2 cut(s)	1125, 1774
PspLI	CGTACG	1 cut(s)	306
PspN4I	GGNNCC	2 cut(s)	7, 609
PspPI	GGNCC	3 cut(s)	726, 1036, 1840
PsrI	GAACNNNNNNTAC	2 cut(s)	377, 409
PstI	CTGCAG	1 cut(s)	43
PstNI	CAGNNNCTG	1 cut(s)	644
PsuI	RGATCY	4 cut(s)	5, 565, 1723, 2179
PsyI	GACNNNGTC	3 cut(s)	971, 1844, 2948
RsaI	GTAC	8 cut(s)	134, 308, 386, 500, 776, 1698, 1754, 2216
RsaNI	GTAC	8 cut(s)	133, 307, 385, 499, 775, 1697, 1753, 2215
RseI	CAYNNNNRTG	2 cut(s)	602, 1418
SacI	GAGCTC	1 cut(s)	2520
SalI	GTCGAC	1 cut(s)	2067
SapI	GCTCTTC	2 cut(s)	2374, 2645
Sau3AI	GATC	9 cut(s)	5, 506, 565, 1591, 1723, 2092, 2179, 2340, 2899
Sau96I	GGNCC	3 cut(s)	726, 1036, 1840
ScaI	AGTACT	1 cut(s)	1754
SchI	GAGTC	3 cut(s)	29, 263, 2074
ScrFI	CCNGG	3 cut(s)	1127, 1388, 1776
SduI	GDGCHC	3 cut(s)	123, 651, 2520
SexAI	ACCWGGT	1 cut(s)	1774
SfaNI	GCATC	4 cut(s)	1180, 1449, 1891, 2596
SfcI	CTRYAG	1 cut(s)	39
Sfr274I	CTCGAG	1 cut(s)	2112
SinI	GGWCC	2 cut(s)	1036, 1840
SlaI	CTCGAG	1 cut(s)	2112
SmiMI	CAYNNNNRTG	2 cut(s)	602, 1418
SmlI	CTYRAG	6 cut(s)	569, 1631, 2112, 2303, 2399, 2687
SmoI	CTYRAG	6 cut(s)	569, 1631, 2112, 2303, 2399, 2687
SphI	GCATGC	1 cut(s)	496
SsiI	CCGC	9 cut(s)	155, 158, 161, 164, 167, 186, 912, 1821, 2590
SspMI	CTAG	6 cut(s)	59, 1436, 1694, 1950, 2429, 2442
SstI	GAGCTC	1 cut(s)	2520
StyD4I	CCNGG	3 cut(s)	1125, 1386, 1774
StyI	CCWWGG	1 cut(s)	2618
TaaI	ACNGT	6 cut(s)	295, 646, 1294, 1490, 1888, 1936
TaiI	ACGT	5 cut(s)	129, 150, 343, 1405, 2416
TaqI	TCGA	9 cut(s)	210, 444, 691, 2036, 2068, 2113, 2372, 2712, 2814
TatI	WGTACW	5 cut(s)	384, 498, 774, 1696, 1752
TauI	GCSGC	5 cut(s)	160, 163, 166, 169, 914
TfiI	GAWTC	7 cut(s)	1186, 1522, 1646, 1922, 2167, 2200, 2906
TscAI	CASTG	6 cut(s)	358, 1057, 1354, 1495, 1941, 2130
TseFI	GTSAC	3 cut(s)	514, 1846, 2280
TseI	GCWGC	7 cut(s)	41, 44, 857, 2333, 2753, 2876, 2957
Tsp45I	GTSAC	3 cut(s)	514, 1846, 2280
TspDTI	ATGAA	9 cut(s)	117, 677, 804, 1487, 1827, 2210, 2283, 2409, 2718
TspGWI	ACGGA	3 cut(s)	247, 491, 731
TspRI	CASTG	6 cut(s)	358, 1057, 1354, 1495, 1941, 2130
Tth111I	GACNNNGTC	3 cut(s)	971, 1844, 2948
Van91I	CCANNNNNTGG	2 cut(s)	1080, 2224
Vha464I	CTTAAG	1 cut(s)	2399
VneI	GTGCAC	1 cut(s)	119
VpaK11BI	GGWCC	2 cut(s)	1036, 1840
VspI	ATTAAT	1 cut(s)	2055
XapI	RAATTY	6 cut(s)	194, 662, 1316, 1555, 2780, 2912
XceI	RCATGY	3 cut(s)	496, 1282, 1516
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1606
XhoI	CTCGAG	1 cut(s)	2112
XmiI	GTMKAC	2 cut(s)	713, 2068
XmnI	GAANNNNTTC	1 cut(s)	2856
XspI	CTAG	6 cut(s)	59, 1436, 1694, 1950, 2429, 2442
ZrmI	AGTACT	1 cut(s)	1754
Zsp2I	ATGCAT	2 cut(s)	242, 1415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.