Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0015112

Physical Location & Seq

Genomic Coordinates Reverse (-)

30561527 .. 30567580

Gene Structure 6054 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002249

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2979 bp

ATGGGATCCCTGAAGAGAAAGTCGGAGGAAGATGACCCTGCAGCAGCTCCACCAAAAGTAGCCAAGTCGGAGAACAAGAATACTCTTGAAATCGTCAACGATGAACAGATGGTGTGCGTGCACGACGTGTCGTACCCTGAGGGTTATGTTCCTCCTCCGCGGCCGCCGCCGCCTTCTTCTTCCGAACCCGCCAAGAAATTCCCCTTCACTCTCGACCCTTTTCAGTCCGAAGCCATCAAATGCATTGAAAATGGCGAGTCCGTCATGGTGTCAGCTCACACATCTGCTGGAAAAACTGTTGTTGCGTCATACGCGATTGCGTTGTGTTTGCATAACAAGCAACGTGTGATATACACCGCACCCATCAAAGCTCTCAGCAACCAAAAGTACAGAGAGTTCAAAGAGGAGTTTTCGGATGTTGGGTTGATGACTGGAGATGTAACAATCGATCCCAATGCTTCGTGCTTGGTGATGACCACGGAAATCTGGCGAAGCATGCAGTACAAAGGATCAGAGGTGACACGAGAGGTGGCTTGGATAATCTTTGATGAGGTGCATTACATGCGGGATCTCGAGAGAGGTGTGGTGTGGGAAGAGAGCATTGTTATGGCTCCAAAGAATGCTCGCTTTGTCTTCCTCTCAGCAACTGTGCCCAATGCCAAGGAATTTGCTGATTGGCTTGCAAAGGTTCATCGACAGCCTTGCCACATTGTCTATACGGATTATAGGCCCACTCCTCTTCAGCATTATATTTTCCCCTCCGGAGGGGATGGTCTGTACTTGGTAGTTGATGAAAAAGGAAAGTTTCGTGAGGACAGCTTTCAACAAGCTCTTAATGCACTTGCTCCTGCCTCCGACGCTGCTAAGAAGAAGGAAAATGGCAAGTGGCAGAAGGGTCTGGTCATCGGCAAGGCCGCTGAAGAAAGCGATATCTTCAAGATGGTGAAAATGATAATTCAGCGTCAGTATGACCCTGTCATACTTTTCAGCTTTAGCAAAAGGGAATGTGAATTACTTGCAATGCAGATGGCAAAGTTGGACCTAAATGGGGACAGTGAGAAAGCAAACATAGAAACCATCTTCTGGAGTGCTATGGATATGCTTTCAGACGACGATAAGAAGTTACCCCAGGTTACCACTATGTTACCCCTTTTAATGCGTGGAATAGGTGTGCATCATTCTGGTTTGATTCCCATTCTAAAGGAAGTGATTGAGATATTATTTCAGGAAGGTCTTATCAAGTGTTTGTTTGCAACAGAGACCTTCAGCATAGGACTGAACATGCCTGCGAAAACTGTTGTATTTACCAATGTCCGGAAATTTGATGGTAATAAGTTCAGATGGATAACCAGTGGTGAGTATATACAAATGAGTGGTCGTGCTGGTCGCCGGGGTATTGATAAACGTGGGATATGCATACTTATGGTAGATGAGAAGCTAGAGCCATCTACTGCCAAAATGATGCTTAAAGGAAATGCTGATAGTTTGAACAGTGCCTTTCATTTAAGCTACAACATGCTTCTGAATCAATTGCGCTCTGAAGATGGTAATCCAGAAAATTTACTTCGTAACTCATTCTATCAGTTCCAAGCTGATCGTGCCATTCCCAATCTGGAGAAACAAGCAAAGGAGCTTGAGCAAGAGAGGGATTCAATTATAATTGAAGAAGAAGATAGTGTAAGCAATTATTACAATCTAGTACAGCAATACAAGAGTTTAAAGCAGGATCTTCGTGAGATTGCGATTTCTCCAAAGTACTGCTTACCATTTCTGAAACCTGGTAGGCTTGTGTCAATCCAATGCACCAAGAGTGATGAAGCTTCTGCTTCTTTTTCTACTGAGGACCATGTCACATGGGGTGTTATACTTAATTTTCAACGAGTAAAAACTGTTCCTGAAGACGATGCAGTTAAGAGACCTGAAGATTCAAACTACACAGTGGATGTTTTGACTAGGTGTCTGGTCGGCACAAATGGAGTTGCGAAAAAAACACTCAAAATTGTTCCTTTGAAAGAACTGGGAGAACCTGTTGTTGTTTCGATTTCCATCTCTCAGATTAATAGCATGAGTCGACTTTGTATGGTCATACCAAATGATCTTTTGTCATTACAAGCTCGAGAAAACACACTGAAGAAAGTTCTTGAAACTCTTTCAAGATTTGGAGAGAAGATTCCTCTTTTAGATCCTGAAGAAGACATGAAGATTCAAAGTAGTTCGTACAAAAAAGTGGCTCGTAGGATTGAGGCTTTAGAGAACCTGTTTGACAGGCATGAAATTGCAAAATCACCACTTATTGAGCAAAAGCTCAAGGTTTTTCACATGAAGCAGGAATTGGCAGCGAAGATCAAGTCAATTAAGAAAACAATGCGCTCTTCGACTGCGTTGGCTTTTAAAGATGAACTTAAGGCACGAAAACGGGTTCTTCGGAGGCTAGGATATGTTACTAGCGATGATGTTGTGCAGTTGAAGGGTAAGGTTGCTTGTGAAATCAGTAGTGCAGATGAGTTGACCCTCACAGAGCTCATGTTCAATGGCGTGTTCAAGGACATAAAGGTAGAAGAGATGGTATCTCTTCTCTCATGTTTTGTTTGGCGGGAAAAACTAAAAGATGCTACAAAACCAAGGGAAGAACTTGACCTGCTCTTCTTGCAATTACAAGAAACTGCTCGGAGGGTGGCTGAAGTTCAGCTTGAGTGCAAGGTTGAAATTGACATCGATAGCTTTGTGAGTTCATTTCGGCCTGATATTATGGAGGCTGCATATGCTTGGGCAAAAGGGTCAAAATTTTATGAGATAATGTCAGTTACAGGGGTTTTCGAGGGTAGCTTAATCAGGGCAATCAGGAGATTAGAGGAAGTTCTTCAGCAACTTATACACGCAGCCAATTCTATTGGAGAAACTGACCTTGAATCAAAATTTGAGGAGGCTGTTTCAAAAATCAAGAGAGACATTGTCTTTGCAGCTTCCCTATACTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0022613 GO:0031123 GO:0031125 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

993

Amino Acids

112.27

Weight (kDa)

6.28

Isoelectric Point (pI)

49.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	70 - 217	3e-09	Type III restriction enzyme, res subunit
DEAD	PF00270	73 - 222	7.6e-27	DEAD/DEAH box helicase
DEXQc_Suv3	PF22527	94 - 204	2.2e-07	DEXQ-box helicase domain of Suv3
MTR4-like_stalk	PF21408	519 - 581	2.1e-20	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	583 - 790	7.8e-49	Mtr4-like, beta-barrel domain
DSHCT	PF08148	817 - 987	6.8e-55	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015112)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59760
fragaria_vesca	FvH4_5g28050 FvH4_5g28050 FvH4_5g28050
malus_domestica	MD08G1247600.v1.1
prunus_persica	Prupe.6G113400_v2.0.a1
pyrus_communis	pycom08g21450
rosa_chinensis	RchiOBHm_Chr7g0220221
rosa_laevigata	RLG00000002249
rosa_multiflora	Rmu_sc0001136.1_g000019
rosa_roxburghii	Rroxscaffold_3G00239700
rosa_rugosa	Rorug07G0191200
rosa_samantha	Rh7AG333300 Rh7CG350900 Rh7DG329600
rosa_wichuraiana	Rw7G028110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1658
Acc36I	ACCTGC	1 cut(s)	2646
AccB7I	CCANNNNNTGG	1 cut(s)	1083
AccI	GTMKAC	1 cut(s)	2071
AccII	CGCG	2 cut(s)	160, 314
AccIII	TCCGGA	2 cut(s)	761, 1314
AclWI	GGATC	6 cut(s)	13, 443, 517, 576, 1734, 2177
AcoI	YGGCCR	1 cut(s)	161
AcsI	RAATTY	6 cut(s)	197, 665, 1319, 1558, 2783, 2915
AdeI	CACNNNGTG	1 cut(s)	127
AfaI	GTAC	7 cut(s)	134, 389, 503, 779, 1701, 1757, 2219
AfiI	CCNNNNNNNGG	5 cut(s)	764, 765, 1048, 1083, 1314
AflII	CTTAAG	1 cut(s)	2402
AflIII	ACRYGT	2 cut(s)	126, 343
AhdI	GACNNNNNGTC	1 cut(s)	1956
AjiI	CACGTC	1 cut(s)	127
AjnI	CCWGG	2 cut(s)	1128, 1777
AjuI	GAANNNNNNNTTGG	2 cut(s)	2315, 2347
Alw21I	GWGCWC	2 cut(s)	123, 2523
Alw26I	GTCTC	3 cut(s)	1253, 1909, 2940
Alw44I	GTGCAC	1 cut(s)	119
AlwI	GGATC	6 cut(s)	13, 443, 517, 576, 1734, 2177
AlwNI	CAGNNNCTG	1 cut(s)	647
Ama87I	CYCGRG	2 cut(s)	572, 2115
Aor13HI	TCCGGA	2 cut(s)	761, 1314
AoxI	GGCC	4 cut(s)	161, 728, 912, 2738
ApaLI	GTGCAC	1 cut(s)	119
ApeKI	GCWGC	7 cut(s)	41, 44, 860, 2336, 2756, 2879, 2960
ApoI	RAATTY	6 cut(s)	197, 665, 1319, 1558, 2783, 2915
ArsI	GACNNNNNNTTYG	6 cut(s)	1360, 1392, 2333, 2365, 2939, 2971
AseI	ATTAAT	1 cut(s)	2058
Asp700I	GAANNNNTTC	1 cut(s)	2859
AspLEI	GCGC	2 cut(s)	1536, 2370
AspS9I	GGNCC	3 cut(s)	729, 1039, 1843
AsuC2I	CCSGG	1 cut(s)	1391
AsuHPI	GGTGA	5 cut(s)	481, 529, 955, 1367, 2277
AvaI	CYCGRG	2 cut(s)	572, 2115
AvaII	GGWCC	2 cut(s)	1039, 1843
AxyI	CCTNAGG	1 cut(s)	138
BaeGI	GKGCMC	2 cut(s)	123, 654
BamHI	GGATCC	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	2523
BauI	CACGAG	1 cut(s)	522
BbsI	GAAGAC	3 cut(s)	625, 1905, 2199
Bbv12I	GWGCWC	2 cut(s)	123, 2523
BbvI	GCAGC	7 cut(s)	53, 56, 847, 2348, 2743, 2891, 2972
BcgI	CGANNNNNNTGC	6 cut(s)	437, 471, 684, 718, 1712, 1746
BciT130I	CCWGG	2 cut(s)	1130, 1779
BcnI	CCSGG	1 cut(s)	1391
BcoDI	GTCTC	3 cut(s)	1253, 1909, 2940
BfaI	CTAG	5 cut(s)	1439, 1697, 1953, 2432, 2445
BfmI	CTRYAG	1 cut(s)	39
BfrI	CTTAAG	1 cut(s)	2402
BfuAI	ACCTGC	1 cut(s)	2646
BmcAI	AGTACT	1 cut(s)	1757
Bme1390I	CCNGG	3 cut(s)	1130, 1391, 1779
Bme18I	GGWCC	2 cut(s)	1039, 1843
BmeRI	GACNNNNNGTC	1 cut(s)	1956
BmeT110I	CYCGRG	2 cut(s)	572, 2115
BmgBI	CACGTC	1 cut(s)	127
BmgT120I	GGNCC	3 cut(s)	729, 1039, 1843
BmiI	GGNNCC	2 cut(s)	7, 612
BmrFI	CCNGG	3 cut(s)	1130, 1391, 1779
BmrI	ACTGGG	1 cut(s)	2027
BmsI	GCATC	4 cut(s)	1183, 1452, 1894, 2599
BmuI	ACTGGG	1 cut(s)	2027
BpiI	GAAGAC	3 cut(s)	625, 1905, 2199
BpmI	CTGGAG	3 cut(s)	453, 1105, 1634
BpuEI	CTTGAG	3 cut(s)	1655, 2291, 2711
BpuMI	CCSGG	1 cut(s)	1391
Bsa29I	ATCGAT	2 cut(s)	447, 2715
BsaBI	GATNNNNATC	2 cut(s)	1548, 2045
BsaI	GGTCTC	2 cut(s)	1253, 1909
BsaJI	CCNNGG	6 cut(s)	158, 477, 660, 1128, 1390, 2621
BsaWI	WCCGGW	2 cut(s)	761, 1314
BsaXI	ACNNNNNCTCC	2 cut(s)	2013, 2043
Bsc4I	CCNNNNNNNGG	5 cut(s)	764, 765, 1048, 1083, 1314
Bse1I	ACTGG	3 cut(s)	436, 1350, 2022
Bse21I	CCTNAGG	1 cut(s)	138
Bse3DI	GCAATG	1 cut(s)	1026
Bse8I	GATNNNNATC	2 cut(s)	1548, 2045
BseAI	TCCGGA	2 cut(s)	761, 1314
BseBI	CCWGG	2 cut(s)	1130, 1779
BseCI	ATCGAT	2 cut(s)	447, 2715
BseDI	CCNNGG	6 cut(s)	158, 477, 660, 1128, 1390, 2621
BseGI	GGATG	3 cut(s)	421, 775, 1948
BseJI	GATNNNNATC	2 cut(s)	1548, 2045
BseLI	CCNNNNNNNGG	5 cut(s)	764, 765, 1048, 1083, 1314
BseMI	GCAATG	1 cut(s)	1026
BseMII	CTCAG	5 cut(s)	129, 388, 654, 1830, 2066
BseNI	ACTGG	3 cut(s)	436, 1350, 2022
BseRI	GAGGAG	4 cut(s)	144, 419, 726, 2936
BseSI	GKGCMC	2 cut(s)	123, 654
BseX3I	CGGCCG	1 cut(s)	161
BseXI	GCAGC	7 cut(s)	53, 56, 847, 2348, 2743, 2891, 2972
BsgI	GTGCAG	2 cut(s)	2480, 2517
Bsh1236I	CGCG	2 cut(s)	160, 314
Bsh1285I	CGRYCG	1 cut(s)	164
BshFI	GGCC	4 cut(s)	163, 730, 914, 2740
BshVI	ATCGAT	2 cut(s)	447, 2715
BsiEI	CGRYCG	1 cut(s)	164
BsiHKAI	GWGCWC	2 cut(s)	123, 2523
BsiHKCI	CYCGRG	2 cut(s)	572, 2115
BsiSI	CCGG	3 cut(s)	762, 1315, 1390
BslFI	GGGAC	1 cut(s)	1064
BslI	CCNNNNNNNGG	5 cut(s)	764, 765, 1048, 1083, 1314
BsmAI	GTCTC	3 cut(s)	1253, 1909, 2940
BsmFI	GGGAC	1 cut(s)	1064
BsmI	GAATGC	1 cut(s)	625
BsnI	GGCC	4 cut(s)	163, 730, 914, 2740
Bso31I	GGTCTC	2 cut(s)	1253, 1909
BsoBI	CYCGRG	2 cut(s)	572, 2115
Bsp1286I	GDGCHC	3 cut(s)	123, 654, 2523
Bsp13I	TCCGGA	2 cut(s)	761, 1314
Bsp143I	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
BspANI	GGCC	4 cut(s)	163, 730, 914, 2740
BspCNI	CTCAG	5 cut(s)	130, 387, 653, 1831, 2065
BspDI	ATCGAT	2 cut(s)	447, 2715
BspEI	TCCGGA	2 cut(s)	761, 1314
BspFNI	CGCG	2 cut(s)	160, 314
BspLI	GGNNCC	2 cut(s)	7, 612
BspMAI	CTGCAG	1 cut(s)	43
BspMI	ACCTGC	1 cut(s)	2646
BspPI	GGATC	6 cut(s)	13, 443, 517, 576, 1734, 2177
BspQI	GCTCTTC	2 cut(s)	2377, 2648
BspTI	CTTAAG	1 cut(s)	2402
BspTNI	GGTCTC	2 cut(s)	1253, 1909
BsrDI	GCAATG	1 cut(s)	1026
BsrI	ACTGG	3 cut(s)	436, 1350, 2022
BssECI	CCNNGG	6 cut(s)	158, 477, 660, 1128, 1390, 2621
BssMI	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	2 cut(s)	660, 2621
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	2 cut(s)	1130, 1779
Bst4CI	ACNGT	7 cut(s)	298, 649, 1055, 1297, 1493, 1891, 1939
Bst6I	CTCTTC	7 cut(s)	8, 588, 744, 2377, 2553, 2577, 2648
BstAFI	CTTAAG	1 cut(s)	2402
BstAPI	GCANNNNNTGC	1 cut(s)	562
BstC8I	GCNNGC	5 cut(s)	119, 497, 625, 681, 1287
BstDEI	CTNAG	6 cut(s)	138, 374, 640, 864, 1839, 2052
BstDSI	CCRYGG	2 cut(s)	158, 477
BstEII	GGTNACC	1 cut(s)	1132
BstF5I	GGATG	3 cut(s)	421, 775, 1948
BstFNI	CGCG	2 cut(s)	160, 314
BstHHI	GCGC	2 cut(s)	1536, 2370
BstKTI	GATC	9 cut(s)	8, 451, 512, 571, 1597, 1729, 2098, 2185, 2346
BstMAI	GTCTC	3 cut(s)	1253, 1909, 2940
BstMBI	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
BstMCI	CGRYCG	1 cut(s)	164
BstNI	CCWGG	2 cut(s)	1130, 1779
BstNSI	RCATGY	4 cut(s)	499, 565, 1285, 1519
BstPI	GGTNACC	1 cut(s)	1132
BstSCI	CCNGG	3 cut(s)	1128, 1389, 1777
BstSFI	CTRYAG	1 cut(s)	39
BstSLI	GKGCMC	2 cut(s)	123, 654
BstUI	CGCG	2 cut(s)	160, 314
BstV1I	GCAGC	7 cut(s)	53, 56, 847, 2348, 2743, 2891, 2972
BstV2I	GAAGAC	3 cut(s)	625, 1905, 2199
BstX2I	RGATCY	4 cut(s)	5, 568, 1726, 2182
BstYI	RGATCY	4 cut(s)	5, 568, 1726, 2182
BstZI	CGGCCG	1 cut(s)	161
Bsu15I	ATCGAT	2 cut(s)	447, 2715
Bsu36I	CCTNAGG	1 cut(s)	138
BsuRI	GGCC	4 cut(s)	163, 730, 914, 2740
BsuTUI	ATCGAT	2 cut(s)	447, 2715
BtgI	CCRYGG	2 cut(s)	158, 477
BtgZI	GCGATG	1 cut(s)	2463
BtrI	CACGTC	1 cut(s)	127
BtsCI	GGATG	3 cut(s)	421, 775, 1948
BtsIMutI	CAGTG	5 cut(s)	1060, 1357, 1498, 1944, 2126
BveI	ACCTGC	1 cut(s)	2646
Cac8I	GCNNGC	5 cut(s)	119, 497, 625, 681, 1287
CaiI	CAGNNNCTG	1 cut(s)	647
CciNI	GCGGCCGC	1 cut(s)	161
CfoI	GCGC	2 cut(s)	1536, 2370
Cfr13I	GGNCC	3 cut(s)	729, 1039, 1843
Cfr42I	CCGCGG	1 cut(s)	161
ClaI	ATCGAT	2 cut(s)	447, 2715
CseI	GACGC	3 cut(s)	294, 866, 950
CsiI	ACCWGGT	1 cut(s)	1777
Csp6I	GTAC	7 cut(s)	133, 388, 502, 778, 1700, 1756, 2218
CviQI	GTAC	7 cut(s)	133, 388, 502, 778, 1700, 1756, 2218
DdeI	CTNAG	6 cut(s)	138, 374, 640, 864, 1839, 2052
DpnI	GATC	9 cut(s)	7, 450, 511, 570, 1596, 1728, 2097, 2184, 2345
DpnII	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
DraI	TTTAAA	2 cut(s)	1719, 2392
DraIII	CACNNNGTG	1 cut(s)	127
DriI	GACNNNNNGTC	1 cut(s)	1956
EaeI	YGGCCR	1 cut(s)	161
EagI	CGGCCG	1 cut(s)	161
Eam1104I	CTCTTC	7 cut(s)	8, 588, 744, 2377, 2553, 2577, 2648
Eam1105I	GACNNNNNGTC	1 cut(s)	1956
EarI	CTCTTC	7 cut(s)	8, 588, 744, 2377, 2553, 2577, 2648
Ecl136II	GAGCTC	1 cut(s)	2521
EclXI	CGGCCG	1 cut(s)	161
Eco130I	CCWWGG	2 cut(s)	660, 2621
Eco24I	GRGCYC	1 cut(s)	2523
Eco31I	GGTCTC	2 cut(s)	1253, 1909
Eco32I	GATATC	1 cut(s)	931
Eco47I	GGWCC	2 cut(s)	1039, 1843
Eco52I	CGGCCG	1 cut(s)	161
Eco53kI	GAGCTC	1 cut(s)	2521
Eco81I	CCTNAGG	1 cut(s)	138
Eco88I	CYCGRG	2 cut(s)	572, 2115
Eco91I	GGTNACC	1 cut(s)	1132
EcoICRI	GAGCTC	1 cut(s)	2521
EcoO65I	GGTNACC	1 cut(s)	1132
EcoRII	CCWGG	2 cut(s)	1128, 1777
EcoRV	GATATC	1 cut(s)	931
EcoT14I	CCWWGG	2 cut(s)	660, 2621
EcoT22I	ATGCAT	2 cut(s)	245, 1418
EcoT38I	GRGCYC	1 cut(s)	2523
ErhI	CCWWGG	2 cut(s)	660, 2621
FalI	AAGNNNNNCTT	6 cut(s)	1745, 1777, 2385, 2417, 2673, 2705
FaqI	GGGAC	1 cut(s)	1064
FauI	CCCGC	3 cut(s)	196, 558, 2586
FauNDI	CATATG	1 cut(s)	2761
FblI	GTMKAC	1 cut(s)	2071
FokI	GGATG	3 cut(s)	428, 782, 1955
FriOI	GRGCYC	1 cut(s)	2523
FspBI	CTAG	5 cut(s)	1439, 1697, 1953, 2432, 2445
GlaI	GCGC	2 cut(s)	1535, 2369
GsuI	CTGGAG	3 cut(s)	453, 1105, 1634
HaeIII	GGCC	4 cut(s)	163, 730, 914, 2740
HapII	CCGG	3 cut(s)	762, 1315, 1390
HgaI	GACGC	3 cut(s)	294, 866, 950
HhaI	GCGC	2 cut(s)	1536, 2370
Hin6I	GCGC	2 cut(s)	1534, 2368
HinP1I	GCGC	2 cut(s)	1534, 2368
HincII	GTYRAC	3 cut(s)	97, 2072, 2508
HindII	GTYRAC	3 cut(s)	97, 2072, 2508
HindIII	AAGCTT	1 cut(s)	1818
HinfI	GANTC	9 cut(s)	257, 1189, 1525, 1649, 1925, 2068, 2170, 2203, 2909
HpaII	CCGG	3 cut(s)	762, 1315, 1390
HphI	GGTGA	5 cut(s)	481, 529, 955, 1367, 2277
Hpy166II	GTNNAC	4 cut(s)	97, 121, 2072, 2508
Hpy8I	GTNNAC	4 cut(s)	97, 121, 2072, 2508
Hpy99I	CGWCG	3 cut(s)	128, 860, 1115
HpyAV	CCTTC	7 cut(s)	183, 214, 865, 886, 1223, 1273, 2461
HpyCH4III	ACNGT	7 cut(s)	298, 649, 1055, 1297, 1493, 1891, 1939
HpyCH4IV	ACGT	3 cut(s)	126, 343, 1405
HpyF3I	CTNAG	6 cut(s)	138, 374, 640, 864, 1839, 2052
HpySE526I	ACGT	3 cut(s)	126, 343, 1405
HspAI	GCGC	2 cut(s)	1534, 2368
Kpn2I	TCCGGA	2 cut(s)	761, 1314
KspI	CCGCGG	1 cut(s)	161
Kzo9I	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
LguI	GCTCTTC	2 cut(s)	2377, 2648
LmnI	GCTCC	4 cut(s)	52, 616, 850, 1630
Lsp1109I	GCAGC	7 cut(s)	53, 56, 847, 2348, 2743, 2891, 2972
LweI	GCATC	4 cut(s)	1183, 1452, 1894, 2599
MabI	ACCWGGT	1 cut(s)	1777
MaeI	CTAG	5 cut(s)	1439, 1697, 1953, 2432, 2445
MaeII	ACGT	3 cut(s)	126, 343, 1405
MaeIII	GTNAC	9 cut(s)	439, 517, 1122, 1132, 1143, 1568, 1849, 2440, 2803
MalI	GATC	9 cut(s)	7, 450, 511, 570, 1596, 1728, 2097, 2184, 2345
MboI	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
MfeI	CAATTG	1 cut(s)	1529
MflI	RGATCY	4 cut(s)	5, 568, 1726, 2182
MhlI	GDGCHC	3 cut(s)	123, 654, 2523
MlyI	GAGTC	2 cut(s)	266, 2077
MmeI	TCCRAC	3 cut(s)	48, 879, 1017
Mph1103I	ATGCAT	2 cut(s)	245, 1418
MroI	TCCGGA	2 cut(s)	761, 1314
MroXI	GAANNNNTTC	1 cut(s)	2859
MslI	CAYNNNNRTG	2 cut(s)	605, 1421
MspA1I	CMGCKG	2 cut(s)	160, 917
MspCI	CTTAAG	1 cut(s)	2402
MspI	CCGG	3 cut(s)	762, 1315, 1390
MspR9I	CCNGG	3 cut(s)	1130, 1391, 1779
MunI	CAATTG	1 cut(s)	1529
Mva1269I	GAATGC	1 cut(s)	625
MvaI	CCWGG	2 cut(s)	1130, 1779
MvnI	CGCG	2 cut(s)	160, 314
NciI	CCSGG	1 cut(s)	1391
NdeI	CATATG	1 cut(s)	2761
NdeII	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
NlaIV	GGNNCC	2 cut(s)	7, 612
NmuCI	GTSAC	2 cut(s)	517, 1849
NotI	GCGGCCGC	1 cut(s)	161
NsiI	ATGCAT	2 cut(s)	245, 1418
NspI	RCATGY	4 cut(s)	499, 565, 1285, 1519
PaeI	GCATGC	1 cut(s)	499
PaeR7I	CTCGAG	2 cut(s)	572, 2115
PciSI	GCTCTTC	2 cut(s)	2377, 2648
PctI	GAATGC	1 cut(s)	625
PdmI	GAANNNNTTC	1 cut(s)	2859
PfeI	GAWTC	7 cut(s)	1189, 1525, 1649, 1925, 2170, 2203, 2909
PflFI	GACNNNGTC	3 cut(s)	974, 1847, 2951
PflMI	CCANNNNNTGG	1 cut(s)	1083
PleI	GAGTC	2 cut(s)	265, 2076
PpsI	GAGTC	2 cut(s)	265, 2076
PshBI	ATTAAT	1 cut(s)	2058
PsiI	TTATAA	1 cut(s)	1658
Psp124BI	GAGCTC	1 cut(s)	2523
Psp6I	CCWGG	2 cut(s)	1128, 1777
PspEI	GGTNACC	1 cut(s)	1132
PspGI	CCWGG	2 cut(s)	1128, 1777
PspN4I	GGNNCC	2 cut(s)	7, 612
PspPI	GGNCC	3 cut(s)	729, 1039, 1843
PsrI	GAACNNNNNNTAC	4 cut(s)	380, 412, 1875, 1907
PstI	CTGCAG	1 cut(s)	43
PstNI	CAGNNNCTG	1 cut(s)	647
PsuI	RGATCY	4 cut(s)	5, 568, 1726, 2182
PsyI	GACNNNGTC	3 cut(s)	974, 1847, 2951
RsaI	GTAC	7 cut(s)	134, 389, 503, 779, 1701, 1757, 2219
RsaNI	GTAC	7 cut(s)	133, 388, 502, 778, 1700, 1756, 2218
RseI	CAYNNNNRTG	2 cut(s)	605, 1421
SacI	GAGCTC	1 cut(s)	2523
SacII	CCGCGG	1 cut(s)	161
SalI	GTCGAC	1 cut(s)	2070
SapI	GCTCTTC	2 cut(s)	2377, 2648
Sau3AI	GATC	9 cut(s)	5, 448, 509, 568, 1594, 1726, 2095, 2182, 2343
Sau96I	GGNCC	3 cut(s)	729, 1039, 1843
ScaI	AGTACT	1 cut(s)	1757
SchI	GAGTC	2 cut(s)	266, 2077
ScrFI	CCNGG	3 cut(s)	1130, 1391, 1779
SduI	GDGCHC	3 cut(s)	123, 654, 2523
SexAI	ACCWGGT	1 cut(s)	1777
SfaNI	GCATC	4 cut(s)	1183, 1452, 1894, 2599
SfcI	CTRYAG	1 cut(s)	39
Sfr274I	CTCGAG	2 cut(s)	572, 2115
Sfr303I	CCGCGG	1 cut(s)	161
SgrBI	CCGCGG	1 cut(s)	161
SinI	GGWCC	2 cut(s)	1039, 1843
SlaI	CTCGAG	2 cut(s)	572, 2115
SmiMI	CAYNNNNRTG	2 cut(s)	605, 1421
SmlI	CTYRAG	6 cut(s)	572, 1634, 2115, 2306, 2402, 2690
SmoI	CTYRAG	6 cut(s)	572, 1634, 2115, 2306, 2402, 2690
SphI	GCATGC	1 cut(s)	499
SspMI	CTAG	5 cut(s)	1439, 1697, 1953, 2432, 2445
SstI	GAGCTC	1 cut(s)	2523
StyD4I	CCNGG	3 cut(s)	1128, 1389, 1777
StyI	CCWWGG	2 cut(s)	660, 2621
TaaI	ACNGT	7 cut(s)	298, 649, 1055, 1297, 1493, 1891, 1939
TaiI	ACGT	3 cut(s)	129, 346, 1408
TatI	WGTACW	5 cut(s)	387, 501, 777, 1699, 1755
TauI	GCSGC	5 cut(s)	163, 166, 169, 172, 917
TfiI	GAWTC	7 cut(s)	1189, 1525, 1649, 1925, 2170, 2203, 2909
TscAI	CASTG	5 cut(s)	1060, 1357, 1498, 1944, 2133
TseFI	GTSAC	2 cut(s)	517, 1849
TseI	GCWGC	7 cut(s)	41, 44, 860, 2336, 2756, 2879, 2960
Tsp45I	GTSAC	2 cut(s)	517, 1849
TspGWI	ACGGA	3 cut(s)	250, 494, 734
TspRI	CASTG	5 cut(s)	1060, 1357, 1498, 1944, 2133
Tth111I	GACNNNGTC	3 cut(s)	974, 1847, 2951
Van91I	CCANNNNNTGG	1 cut(s)	1083
Vha464I	CTTAAG	1 cut(s)	2402
VneI	GTGCAC	1 cut(s)	119
VpaK11BI	GGWCC	2 cut(s)	1039, 1843
VspI	ATTAAT	1 cut(s)	2058
XapI	RAATTY	6 cut(s)	197, 665, 1319, 1558, 2783, 2915
XceI	RCATGY	4 cut(s)	499, 565, 1285, 1519
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1609
XhoI	CTCGAG	2 cut(s)	572, 2115
XmiI	GTMKAC	1 cut(s)	2071
XmnI	GAANNNNTTC	1 cut(s)	2859
XspI	CTAG	5 cut(s)	1439, 1697, 1953, 2432, 2445
ZrmI	AGTACT	1 cut(s)	1757
Zsp2I	ATGCAT	2 cut(s)	245, 1418

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.