Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0015112

Physical Location & Seq

Genomic Coordinates Forward (+)

31124091 .. 31131039

Gene Structure 6949 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1247600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3054 bp

ATGGAAACGGAAGCGGCATCCCTGAAGAGGAAGTCGGAGGGAGAGGCCGAGTTGGAAGAAGCTCCACAGAAACAACAAAAGAGGGACAACGGTTGGGCAAGCGTCGACGACGAAGCGGTGGCCTGCTTGCACGACGTGTCGTATCCTGAGGGCTATGTCGTTCCTCCCCCTTCTTCTTCTTCTTCAGCCGCAGCAGAAGCTTCTGAACCTGCCAAGAAATTCCCCTTCCCTCTCGACCCTTTTCAGTCTGAGGCCATAAACTGCCTCGAAAAAGCCGAGTCCGTCATGGTGTCTGCTCATACCTCGGCTGGGAAAACTGTGGTAGCATCGTATGCGATTGCAATGTCTCTAAGAAACAAGCAGAGAGTGATATACACTTCACCAATCAAAGCACTGAGCAACCAAAAGTATAGGGAGTTCAAAGAGGAGTTTTCTGACGTTGGCTTGATGACTGGCGATGTAACTATCGATCCCAATGCTTCCTGCTTGGTTATGACCACAGAAATCTGGCGTAGCATGCAGTACAAGGGGTCAGAGATAACGCGTGAGGTGGCGTGGATTATCTTCGATGAGGTGCATTATATGCGTGATAGGGAGAGAGGTGTGGTGTGGGAAGAGAGCATTGTTATGGCTCCAAAGAATGCTCGGTTTGTGTTCCTCTCAGCAACTGTGCCAAATGCTAAGGAATTTGCTGATTGGCTTGCAAAGATTCACCGTCAGCCTTGTCACATTGTCTATACTGATTATAGGCCAACTCCTCTGCAGCATTATATTTTCCCTTCTGGAGGGAATGGTTTGTACTTGGTAGTAGATGAAAAAGGGAAATTTCGCGAAGACAGCTTTCAGAAAGCTCTTAATGCTCTTGCTCCCGCTGCCGATAGTGCTAAGAAGAAGGAAAATGGCAAGTGGCAGAAGGGTTTGATCATGGGCAAGGCTGCTGAGGAAAGTGATATCTTCAAGATGGTCAAAATGATAATTCAGCGTCAGTATGATCCTGTAATACTTTTTAGCTTTAGCAAAAGGGAGTGCGAATCTCTTGCAATGCAGATGGCAAAATTGGATCTAAATGGGGACAATGAGAAAGCAAATGTAGAAACCATCTTCTGGAGTGCTATGGATATGCTTTCTGATGATGATAAAAAGTTACCTCAGGCAAGCTTTCGTTTCTTCCTTTCTGAGCTGGCATGTCTGTTTAAGCATTGTGTTTCACATATGCTACCCCTTTTGAAGCGTGGAATTGGTGTGCATCATTCTGGCTTGCTTCCAATTCTAAAGGAAGTGATTGAGATATTATTTCAGGAAGGTCTAATCAAGTGTTTATTTGCCACAGAGACCTTCAGCATAGGATTGAACATGCCTGCAAAAACTGTTGTGTTTACCAATGTCCGGAAATTTGATGGTGATAAGTTCAGATGGCTATCCAGTGGAGAGTATATACAAATGAGTGGCCGTGCTGGTCGCCGTGGTATTGATAAGCGTGGAATATGTATACTTATGGTAGATGAGAAGCTGGAACCATCTACTGCTAAAATGATGCTTAAAGGAAGTGCTGATTGTTTGAACAGTGCTTTTCATTTGAGCTACAACATGCTTCTGAATCAATTACGCAGCGAAGATGGTAATCCAGAAAATTTGCTTCGTAACTCGTTCTATCAATTTCAAGCTGATCGTGCCATCCCCAGTCTTGAGAAGCAAGCAAAGGATCTTGAGAAAGAGAGGGATTCAATTATAATTGAAGAAGAAGATAGTGTAAAGAATTATTACAATCTGTTACAGCAATACAAGAGTTTGAAACAGGAACTTCGTGATATTGTGCTTTCTCCAAAGTACTGCTTACCATTTCTGAAACCTGGTAGGCTTGTCTCAATACAATGTGCTAAAAGTGATGAAAGTTCCCCTTCTTTTTCCATTGAGGACCCGGTCACATGGGGAGTGATACTAAATTTTCAACGGGTGAAAACTGCTTCAGAAGATGATGCAAGTAGAAAACCCGAAGATTCAAACTACACAGTGGATGTTCTGACAAGGTGCAGGGTGAGTACAGATGAAGTTGCTAAGAAAACCATCAAAATTATCCCTCTCAAAGAGGCAGGAGAACCTGCTGTTGTCTCCATCTCCATATCTCAGATAAATAGTATGAGTGGACTTTGTATGGTCATTCCTAAGGATCTTTTGCCACTACCAGCTCGAGAAAACACACTGAAGAGAGTTCTTGAAACTCTGTCAAGATTTGATAAAGGAAAGATTCCTCTTCTTGATCCAGAAGAAGATATGAAGATTGAAAGTAGTTCATACAAAAAGGTGTCTCGTAGGATCGAGGCTTTAGAGAACCTGTTTGACAGGCATGAAGTTGCTAGAACTCCACTTATTGAGCAAAAGCTTAAGGTTTTTCATATGAAGCAGGATTTGGCTGCCAAGATCAAGTCAATCAAGAAAACAATGCGCTCTTCCACTGCATTAGCTTTTAAAGATGAACTGAAGGCAAGAAAACGGGTCCTTCGGAGGCTAGGATATGTTACAAATGATGGTGTTGTGGAGTTGAAGGGTAAAGTTGCTTGTGAAATCAGTAGTGCAGAAGAGTTGACCCTGACTGAGCTCATGTTCAATGGGGCTTTCAAGGACGTAAACGTGGAAGAGATGGTGTCTCTTCTCTCATGTTTTGTGTGGCAGGAGAAGCTTAAGGAAGCTACAAAACCAAGGGAAGAGCTTGACCTGCTATTTTCACAATTACAAGATACAGCTCGGAGGGTTGCTGAGGTTCAGCTTGAGTGCAAGGTCGAAATTGATGTTGACAGCTTTGTGAGTTCTTTTCGGCCTGATATTATGGAGGCTGTGTATGCTTGGGCAAAAGGGTCCAAATTCTACGAGATCATGTCAGCTACACCTGTTTTTGAGGGCAGCTTGATCAGGGCAATTAGGAGATTAGAGGAAGTCCTTCAGCAACTGATTCAAGCAGCCAAGTCTATTGGAGAAACCGAGCTTGAGTCAAAATTTGAGGAGGCTGTTTCGAAGATCAAGAGGGACATTGTCTTTGCAGCTTCCCTATACTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0022613 GO:0031123 GO:0031125 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1018

Amino Acids

115.06

Weight (kDa)

6.26

Isoelectric Point (pI)

50.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	78 - 224	2.2e-08	Type III restriction enzyme, res subunit
DEAD	PF00270	80 - 229	1.6e-26	DEAD/DEAH box helicase
DEXQc_Suv3	PF22527	100 - 211	4.8e-08	DEXQ-box helicase domain of Suv3
Helicase_C	PF00271	411 - 489	5.2e-06	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	543 - 606	3.4e-20	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	607 - 815	6.3e-48	Mtr4-like, beta-barrel domain
DSHCT	PF08148	842 - 1012	4.6e-56	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015112)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59760
fragaria_vesca	FvH4_5g28050 FvH4_5g28050 FvH4_5g28050
malus_domestica	MD08G1247600.v1.1
prunus_persica	Prupe.6G113400_v2.0.a1
pyrus_communis	pycom08g21450
rosa_chinensis	RchiOBHm_Chr7g0220221
rosa_laevigata	RLG00000002249
rosa_multiflora	Rmu_sc0001136.1_g000019
rosa_roxburghii	Rroxscaffold_3G00239700
rosa_rugosa	Rorug07G0191200
rosa_samantha	Rh7AG333300 Rh7CG350900 Rh7DG329600
rosa_wichuraiana	Rw7G028110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1730
Acc36I	ACCTGC	3 cut(s)	217, 2107, 2721
AccB7I	CCANNNNNTGG	1 cut(s)	1104
AccI	GTMKAC	2 cut(s)	105, 1489
AccII	CGCG	2 cut(s)	544, 831
AccIII	TCCGGA	1 cut(s)	1386
AciI	CCGC	4 cut(s)	14, 116, 189, 870
AclWI	GGATC	7 cut(s)	464, 986, 1068, 1710, 2175, 2252, 2321
AcoI	YGGCCR	1 cut(s)	1447
AcsI	RAATTY	8 cut(s)	218, 686, 824, 1391, 1630, 1942, 2858, 2990
AcuI	CTGAAG	7 cut(s)	44, 168, 1321, 1950, 2222, 2498, 2921
AdeI	CACNNNGTG	1 cut(s)	136
AfaI	GTAC	4 cut(s)	524, 800, 1829, 2041
AfiI	CCNNNNNNNGG	5 cut(s)	27, 309, 785, 1104, 1386
AflII	CTTAAG	2 cut(s)	2381, 2678
AflIII	ACRYGT	2 cut(s)	135, 542
AjiI	CACGTC	1 cut(s)	136
AjnI	CCWGG	1 cut(s)	1849
AjuI	GAANNNNNNNTTGG	2 cut(s)	2390, 2422
Alw21I	GWGCWC	1 cut(s)	2598
Alw26I	GTCTC	6 cut(s)	351, 1325, 1867, 2113, 2310, 2649
AlwI	GGATC	7 cut(s)	464, 986, 1068, 1710, 2175, 2252, 2321
AlwNI	CAGNNNCTG	2 cut(s)	668, 2944
Ama87I	CYCGRG	1 cut(s)	2187
Aor13HI	TCCGGA	1 cut(s)	1386
AoxI	GGCC	6 cut(s)	45, 120, 252, 749, 1447, 2813
ApeKI	GCWGC	9 cut(s)	191, 763, 872, 935, 1608, 2411, 2898, 2954, 3035
ApoI	RAATTY	8 cut(s)	218, 686, 824, 1391, 1630, 1942, 2858, 2990
ArsI	GACNNNNNNTTYG	2 cut(s)	3014, 3046
Asp700I	GAANNNNTTC	1 cut(s)	2934
AspLEI	GCGC	1 cut(s)	2445
AspS9I	GGNCC	3 cut(s)	1915, 2494, 2853
AsuC2I	CCSGG	1 cut(s)	1919
AsuHPI	GGTGA	5 cut(s)	372, 704, 1412, 1966, 2047
AsuII	TTCGAA	1 cut(s)	3008
AvaI	CYCGRG	1 cut(s)	2187
AvaII	GGWCC	3 cut(s)	1915, 2494, 2853
AxyI	CCTNAGG	3 cut(s)	147, 1149, 2163
BanII	GRGCYC	1 cut(s)	2598
BbsI	GAAGAC	1 cut(s)	840
Bbv12I	GWGCWC	1 cut(s)	2598
BbvCI	CCTCAGC	2 cut(s)	939, 2754
BbvI	GCAGC	9 cut(s)	203, 775, 859, 922, 1620, 2398, 2910, 2966, 3047
BceAI	ACGGC	2 cut(s)	1434, 1446
BcgI	CGANNNNNNTGC	4 cut(s)	458, 492, 1019, 1053
BciT130I	CCWGG	1 cut(s)	1851
BciVI	GTATCC	1 cut(s)	153
BclI	TGATCA	2 cut(s)	921, 2904
BcnI	CCSGG	1 cut(s)	1919
BcoDI	GTCTC	6 cut(s)	351, 1325, 1867, 2113, 2310, 2649
BfaI	CTAG	2 cut(s)	2355, 2507
BfmI	CTRYAG	1 cut(s)	761
BfrI	CTTAAG	2 cut(s)	2381, 2678
BfuAI	ACCTGC	3 cut(s)	217, 2107, 2721
BfuI	GTATCC	1 cut(s)	153
BmcAI	AGTACT	1 cut(s)	1829
Bme1390I	CCNGG	2 cut(s)	1851, 1919
Bme18I	GGWCC	3 cut(s)	1915, 2494, 2853
BmeT110I	CYCGRG	1 cut(s)	2187
BmgBI	CACGTC	1 cut(s)	136
BmgT120I	GGNCC	3 cut(s)	1915, 2494, 2853
BmiI	GGNNCC	5 cut(s)	633, 1515, 1917, 2495, 2854
BmrFI	CCNGG	2 cut(s)	1851, 1919
BmrI	ACTGGG	1 cut(s)	1674
BmsI	GCATC	5 cut(s)	26, 335, 1255, 1524, 1966
BmuI	ACTGGG	1 cut(s)	1674
BpiI	GAAGAC	1 cut(s)	840
BpmI	CTGGAG	2 cut(s)	804, 1126
Bpu10I	CCTNAGC	3 cut(s)	681, 939, 2754
Bpu14I	TTCGAA	1 cut(s)	3008
BpuEI	CTTGAG	4 cut(s)	1706, 1727, 2786, 3002
BpuMI	CCSGG	1 cut(s)	1919
Bsa29I	ATCGAT	1 cut(s)	468
BsaBI	GATNNNNATC	2 cut(s)	1417, 1620
BsaI	GGTCTC	1 cut(s)	1325
BsaJI	CCNNGG	3 cut(s)	303, 1462, 2696
BsaWI	WCCGGW	1 cut(s)	1386
BsaXI	ACNNNNNCTCC	2 cut(s)	2819, 2849
Bsc4I	CCNNNNNNNGG	5 cut(s)	27, 309, 785, 1104, 1386
Bse1I	ACTGG	3 cut(s)	457, 1422, 1680
Bse21I	CCTNAGG	3 cut(s)	147, 1149, 2163
Bse3DI	GCAATG	2 cut(s)	348, 1047
Bse8I	GATNNNNATC	2 cut(s)	1417, 1620
BseAI	TCCGGA	1 cut(s)	1386
BseBI	CCWGG	1 cut(s)	1851
BseCI	ATCGAT	1 cut(s)	468
BseDI	CCNNGG	3 cut(s)	303, 1462, 2696
BseGI	GGATG	3 cut(s)	17, 1674, 2020
BseJI	GATNNNNATC	2 cut(s)	1417, 1620
BseLI	CCNNNNNNNGG	5 cut(s)	27, 309, 785, 1104, 1386
BseMI	GCAATG	2 cut(s)	348, 1047
BseNI	ACTGG	3 cut(s)	457, 1422, 1680
BseRI	GAGGAG	3 cut(s)	440, 747, 3011
BseXI	GCAGC	9 cut(s)	203, 775, 859, 922, 1620, 2398, 2910, 2966, 3047
BseYI	CCCAGC	1 cut(s)	308
BsgI	GTGCAG	2 cut(s)	2050, 2592
Bsh1236I	CGCG	2 cut(s)	544, 831
BshFI	GGCC	6 cut(s)	47, 122, 254, 751, 1449, 2815
BshVI	ATCGAT	1 cut(s)	468
BsiHKAI	GWGCWC	1 cut(s)	2598
BsiHKCI	CYCGRG	1 cut(s)	2187
BsiSI	CCGG	2 cut(s)	1387, 1919
BslFI	GGGAC	3 cut(s)	98, 1085, 3035
BslI	CCNNNNNNNGG	5 cut(s)	27, 309, 785, 1104, 1386
BsmAI	GTCTC	6 cut(s)	351, 1325, 1867, 2113, 2310, 2649
BsmFI	GGGAC	3 cut(s)	98, 1085, 3035
BsmI	GAATGC	1 cut(s)	646
BsnI	GGCC	6 cut(s)	47, 122, 254, 751, 1449, 2815
Bso31I	GGTCTC	1 cut(s)	1325
BsoBI	CYCGRG	1 cut(s)	2187
Bsp119I	TTCGAA	1 cut(s)	3008
Bsp1286I	GDGCHC	1 cut(s)	2598
Bsp13I	TCCGGA	1 cut(s)	1386
Bsp68I	TCGCGA	1 cut(s)	831
BspACI	CCGC	4 cut(s)	14, 116, 189, 870
BspANI	GGCC	6 cut(s)	47, 122, 254, 751, 1449, 2815
BspDI	ATCGAT	1 cut(s)	468
BspEI	TCCGGA	1 cut(s)	1386
BspFNI	CGCG	2 cut(s)	544, 831
BspLI	GGNNCC	5 cut(s)	633, 1515, 1917, 2495, 2854
BspMAI	CTGCAG	1 cut(s)	765
BspMI	ACCTGC	3 cut(s)	217, 2107, 2721
BspPI	GGATC	7 cut(s)	464, 986, 1068, 1710, 2175, 2252, 2321
BspQI	GCTCTTC	2 cut(s)	2452, 2697
BspT104I	TTCGAA	1 cut(s)	3008
BspTI	CTTAAG	2 cut(s)	2381, 2678
BspTNI	GGTCTC	1 cut(s)	1325
BsrDI	GCAATG	2 cut(s)	348, 1047
BsrI	ACTGG	3 cut(s)	457, 1422, 1680
BssECI	CCNNGG	3 cut(s)	303, 1462, 2696
BssNAI	GTATAC	1 cut(s)	1490
BssT1I	CCWWGG	1 cut(s)	2696
Bst1107I	GTATAC	1 cut(s)	1490
Bst2UI	CCWGG	1 cut(s)	1851
Bst4CI	ACNGT	7 cut(s)	92, 319, 670, 716, 1369, 1565, 2011
Bst6I	CTCTTC	9 cut(s)	20, 609, 2198, 2256, 2452, 2571, 2628, 2652, 2697
BstAFI	CTTAAG	2 cut(s)	2381, 2678
BstAPI	GCANNNNNTGC	2 cut(s)	332, 583
BstBI	TTCGAA	1 cut(s)	3008
BstDSI	CCRYGG	1 cut(s)	1462
BstENI	CCTNNNNNAGG	1 cut(s)	783
BstF5I	GGATG	3 cut(s)	17, 1674, 2020
BstFNI	CGCG	2 cut(s)	544, 831
BstHHI	GCGC	1 cut(s)	2445
BstMAI	GTCTC	6 cut(s)	351, 1325, 1867, 2113, 2310, 2649
BstNI	CCWGG	1 cut(s)	1851
BstNSI	RCATGY	4 cut(s)	520, 1188, 1357, 1591
BstSCI	CCNGG	2 cut(s)	1849, 1917
BstSFI	CTRYAG	1 cut(s)	761
BstUI	CGCG	2 cut(s)	544, 831
BstV1I	GCAGC	9 cut(s)	203, 775, 859, 922, 1620, 2398, 2910, 2966, 3047
BstV2I	GAAGAC	1 cut(s)	840
BstX2I	RGATCY	3 cut(s)	1060, 1702, 2167
BstYI	RGATCY	3 cut(s)	1060, 1702, 2167
BstZ17I	GTATAC	1 cut(s)	1490
Bsu15I	ATCGAT	1 cut(s)	468
Bsu36I	CCTNAGG	3 cut(s)	147, 1149, 2163
BsuI	GTATCC	1 cut(s)	153
BsuRI	GGCC	6 cut(s)	47, 122, 254, 751, 1449, 2815
BsuTUI	ATCGAT	1 cut(s)	468
BtgI	CCRYGG	1 cut(s)	1462
BtgZI	GCGATG	1 cut(s)	471
BtrI	CACGTC	1 cut(s)	136
BtsCI	GGATG	3 cut(s)	17, 1674, 2020
BtsI	GCAGTG	1 cut(s)	2451
BtsIMutI	CAGTG	6 cut(s)	392, 1429, 1570, 2016, 2198, 2451
BtuMI	TCGCGA	1 cut(s)	831
BveI	ACCTGC	3 cut(s)	217, 2107, 2721
CaiI	CAGNNNCTG	2 cut(s)	668, 2944
CfoI	GCGC	1 cut(s)	2445
Cfr13I	GGNCC	3 cut(s)	1915, 2494, 2853
ClaI	ATCGAT	1 cut(s)	468
CseI	GACGC	2 cut(s)	91, 971
CsiI	ACCWGGT	1 cut(s)	1849
Csp6I	GTAC	4 cut(s)	523, 799, 1828, 2040
CviQI	GTAC	4 cut(s)	523, 799, 1828, 2040
DraI	TTTAAA	1 cut(s)	2467
DraIII	CACNNNGTG	1 cut(s)	136
EaeI	YGGCCR	1 cut(s)	1447
Eam1104I	CTCTTC	9 cut(s)	20, 609, 2198, 2256, 2452, 2571, 2628, 2652, 2697
EarI	CTCTTC	9 cut(s)	20, 609, 2198, 2256, 2452, 2571, 2628, 2652, 2697
Ecl136II	GAGCTC	1 cut(s)	2596
Eco130I	CCWWGG	1 cut(s)	2696
Eco24I	GRGCYC	1 cut(s)	2598
Eco31I	GGTCTC	1 cut(s)	1325
Eco32I	GATATC	1 cut(s)	952
Eco47I	GGWCC	3 cut(s)	1915, 2494, 2853
Eco53kI	GAGCTC	1 cut(s)	2596
Eco57I	CTGAAG	7 cut(s)	44, 168, 1321, 1950, 2222, 2498, 2921
Eco81I	CCTNAGG	3 cut(s)	147, 1149, 2163
Eco88I	CYCGRG	1 cut(s)	2187
EcoICRI	GAGCTC	1 cut(s)	2596
EcoNI	CCTNNNNNAGG	1 cut(s)	783
EcoO109I	RGGNCCY	2 cut(s)	1915, 2494
EcoRII	CCWGG	1 cut(s)	1849
EcoRV	GATATC	1 cut(s)	952
EcoT14I	CCWWGG	1 cut(s)	2696
EcoT38I	GRGCYC	1 cut(s)	2598
ErhI	CCWWGG	1 cut(s)	2696
FalI	AAGNNNNNCTT	4 cut(s)	1817, 1849, 1882, 1914
FaqI	GGGAC	3 cut(s)	98, 1085, 3035
FauI	CCCGC	1 cut(s)	877
FauNDI	CATATG	2 cut(s)	1212, 2394
FbaI	TGATCA	2 cut(s)	921, 2904
FblI	GTMKAC	2 cut(s)	105, 1489
FokI	GGATG	3 cut(s)	4, 1661, 2027
FriOI	GRGCYC	1 cut(s)	2598
FspBI	CTAG	2 cut(s)	2355, 2507
GlaI	GCGC	1 cut(s)	2444
GsaI	CCCAGC	1 cut(s)	312
GsuI	CTGGAG	2 cut(s)	804, 1126
HaeIII	GGCC	6 cut(s)	47, 122, 254, 751, 1449, 2815
HapII	CCGG	2 cut(s)	1387, 1919
HgaI	GACGC	2 cut(s)	91, 971
HhaI	GCGC	1 cut(s)	2445
Hin6I	GCGC	1 cut(s)	2443
HinP1I	GCGC	1 cut(s)	2443
HincII	GTYRAC	3 cut(s)	106, 2583, 2791
HindII	GTYRAC	3 cut(s)	106, 2583, 2791
HindIII	AAGCTT	4 cut(s)	198, 1156, 2378, 2675
HinfI	GANTC	9 cut(s)	278, 709, 1031, 1597, 1721, 1997, 2245, 2947, 2984
HpaII	CCGG	2 cut(s)	1387, 1919
HphI	GGTGA	5 cut(s)	372, 704, 1412, 1966, 2047
Hpy166II	GTNNAC	7 cut(s)	106, 1377, 1490, 2144, 2583, 2626, 2791
Hpy8I	GTNNAC	7 cut(s)	106, 1377, 1490, 2144, 2583, 2626, 2791
Hpy99I	CGWCG	4 cut(s)	107, 110, 113, 137
HpyCH4III	ACNGT	7 cut(s)	92, 319, 670, 716, 1369, 1565, 2011
HpyCH4IV	ACGT	4 cut(s)	135, 438, 2622, 2628
HpySE526I	ACGT	4 cut(s)	135, 438, 2622, 2628
HspAI	GCGC	1 cut(s)	2443
Kpn2I	TCCGGA	1 cut(s)	1386
Ksp22I	TGATCA	2 cut(s)	921, 2904
LguI	GCTCTTC	2 cut(s)	2452, 2697
LmnI	GCTCC	3 cut(s)	67, 637, 871
Lsp1109I	GCAGC	9 cut(s)	203, 775, 859, 922, 1620, 2398, 2910, 2966, 3047
LweI	GCATC	5 cut(s)	26, 335, 1255, 1524, 1966
MabI	ACCWGGT	1 cut(s)	1849
MaeI	CTAG	2 cut(s)	2355, 2507
MaeII	ACGT	4 cut(s)	135, 438, 2622, 2628
MaeIII	GTNAC	7 cut(s)	460, 725, 1143, 1640, 1770, 1921, 2515
MflI	RGATCY	3 cut(s)	1060, 1702, 2167
MhlI	GDGCHC	1 cut(s)	2598
MluI	ACGCGT	1 cut(s)	542
MlyI	GAGTC	2 cut(s)	287, 2993
MmeI	TCCRAC	2 cut(s)	15, 33
MroI	TCCGGA	1 cut(s)	1386
MroXI	GAANNNNTTC	1 cut(s)	2934
MseI	TTAA	6 cut(s)	855, 1194, 1539, 2382, 2466, 2679
MslI	CAYNNNNRTG	1 cut(s)	626
MspA1I	CMGCKG	1 cut(s)	872
MspCI	CTTAAG	2 cut(s)	2381, 2678
MspI	CCGG	2 cut(s)	1387, 1919
MspR9I	CCNGG	2 cut(s)	1851, 1919
Mva1269I	GAATGC	1 cut(s)	646
MvaI	CCWGG	1 cut(s)	1851
MvnI	CGCG	2 cut(s)	544, 831
NciI	CCSGG	1 cut(s)	1919
NdeI	CATATG	2 cut(s)	1212, 2394
NlaIV	GGNNCC	5 cut(s)	633, 1515, 1917, 2495, 2854
NmeAIII	GCCGAG	3 cut(s)	73, 284, 301
NmuCI	GTSAC	2 cut(s)	725, 1921
NruI	TCGCGA	1 cut(s)	831
NspI	RCATGY	4 cut(s)	520, 1188, 1357, 1591
NspV	TTCGAA	1 cut(s)	3008
PaeI	GCATGC	1 cut(s)	520
PaeR7I	CTCGAG	1 cut(s)	2187
PciSI	GCTCTTC	2 cut(s)	2452, 2697
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PctI	GAATGC	1 cut(s)	646
PdmI	GAANNNNTTC	1 cut(s)	2934
PfeI	GAWTC	7 cut(s)	709, 1031, 1597, 1721, 1997, 2245, 2947
PflFI	GACNNNGTC	2 cut(s)	1919, 3026
PflMI	CCANNNNNTGG	1 cut(s)	1104
PleI	GAGTC	2 cut(s)	286, 2992
PpsI	GAGTC	2 cut(s)	286, 2992
PpuMI	RGGWCCY	2 cut(s)	1915, 2494
PsiI	TTATAA	1 cut(s)	1730
Psp124BI	GAGCTC	1 cut(s)	2598
Psp5II	RGGWCCY	2 cut(s)	1915, 2494
Psp6I	CCWGG	1 cut(s)	1849
PspFI	CCCAGC	1 cut(s)	308
PspGI	CCWGG	1 cut(s)	1849
PspN4I	GGNNCC	5 cut(s)	633, 1515, 1917, 2495, 2854
PspPI	GGNCC	3 cut(s)	1915, 2494, 2853
PspPPI	RGGWCCY	2 cut(s)	1915, 2494
PsrI	GAACNNNNNNTAC	2 cut(s)	401, 433
PstI	CTGCAG	1 cut(s)	765
PstNI	CAGNNNCTG	2 cut(s)	668, 2944
PsuI	RGATCY	3 cut(s)	1060, 1702, 2167
PsyI	GACNNNGTC	2 cut(s)	1919, 3026
RruI	TCGCGA	1 cut(s)	831
RsaI	GTAC	4 cut(s)	524, 800, 1829, 2041
RsaNI	GTAC	4 cut(s)	523, 799, 1828, 2040
RseI	CAYNNNNRTG	1 cut(s)	626
SacI	GAGCTC	1 cut(s)	2598
SalI	GTCGAC	1 cut(s)	104
SapI	GCTCTTC	2 cut(s)	2452, 2697
SaqAI	TTAA	6 cut(s)	855, 1194, 1539, 2382, 2466, 2679
Sau96I	GGNCC	3 cut(s)	1915, 2494, 2853
ScaI	AGTACT	1 cut(s)	1829
SchI	GAGTC	2 cut(s)	287, 2993
ScrFI	CCNGG	2 cut(s)	1851, 1919
SduI	GDGCHC	1 cut(s)	2598
SexAI	ACCWGGT	1 cut(s)	1849
SfaNI	GCATC	5 cut(s)	26, 335, 1255, 1524, 1966
SfcI	CTRYAG	1 cut(s)	761
Sfr274I	CTCGAG	1 cut(s)	2187
SfuI	TTCGAA	1 cut(s)	3008
SgrDI	CGTCGACG	1 cut(s)	104
SinI	GGWCC	3 cut(s)	1915, 2494, 2853
SlaI	CTCGAG	1 cut(s)	2187
SmiMI	CAYNNNNRTG	1 cut(s)	626
SmlI	CTYRAG	7 cut(s)	1685, 1706, 2187, 2381, 2678, 2765, 2981
SmoI	CTYRAG	7 cut(s)	1685, 1706, 2187, 2381, 2678, 2765, 2981
SphI	GCATGC	1 cut(s)	520
SsiI	CCGC	4 cut(s)	14, 116, 189, 870
SspMI	CTAG	2 cut(s)	2355, 2507
SstI	GAGCTC	1 cut(s)	2598
StyD4I	CCNGG	2 cut(s)	1849, 1917
StyI	CCWWGG	1 cut(s)	2696
TaaI	ACNGT	7 cut(s)	92, 319, 670, 716, 1369, 1565, 2011
TaiI	ACGT	4 cut(s)	138, 441, 2625, 2631
TaqI	TCGA	9 cut(s)	105, 234, 267, 468, 567, 2188, 2316, 2778, 3008
TatI	WGTACW	4 cut(s)	522, 798, 1827, 2039
TauI	GCSGC	2 cut(s)	17, 191
TfiI	GAWTC	7 cut(s)	709, 1031, 1597, 1721, 1997, 2245, 2947
Tru1I	TTAA	6 cut(s)	855, 1194, 1539, 2382, 2466, 2679
Tru9I	TTAA	6 cut(s)	855, 1194, 1539, 2382, 2466, 2679
TscAI	CASTG	6 cut(s)	399, 1429, 1570, 2016, 2205, 2458
TseFI	GTSAC	2 cut(s)	725, 1921
TseI	GCWGC	9 cut(s)	191, 763, 872, 935, 1608, 2411, 2898, 2954, 3035
Tsp45I	GTSAC	2 cut(s)	725, 1921
TspGWI	ACGGA	2 cut(s)	23, 271
TspRI	CASTG	6 cut(s)	399, 1429, 1570, 2016, 2205, 2458
Tth111I	GACNNNGTC	2 cut(s)	1919, 3026
Van91I	CCANNNNNTGG	1 cut(s)	1104
Vha464I	CTTAAG	2 cut(s)	2381, 2678
VpaK11BI	GGWCC	3 cut(s)	1915, 2494, 2853
XagI	CCTNNNNNAGG	1 cut(s)	783
XapI	RAATTY	8 cut(s)	218, 686, 824, 1391, 1630, 1942, 2858, 2990
XceI	RCATGY	4 cut(s)	520, 1188, 1357, 1591
XhoI	CTCGAG	1 cut(s)	2187
XmiI	GTMKAC	2 cut(s)	105, 1489
XmnI	GAANNNNTTC	1 cut(s)	2934
XspI	CTAG	2 cut(s)	2355, 2507
ZrmI	AGTACT	1 cut(s)	1829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.