Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0015112

Physical Location & Seq

Genomic Coordinates Forward (+)

37537989 .. 37544201

Gene Structure 6213 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G028110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2949 bp

ATGGGATCCCTGAAGAGAAAATCGGAGGAAGATGACTCTGCAGCAGCTCCACCCAAACTAGCCAAGTCGGAGAACAAGAACACTCTTGAAATCGTCAACAATGAACAGATGGTCTGCGTGCACGACGTGTCGTACCCTGAGGGTTACGTTCCTCCGCCGCCGCCGCCTTCTTCTTCCGAACCCGCCAAGAAATTCCCCTTCACTCTCGACCCTTTTCAGTCCGAAGCCATCAAATGCATTGAAAATGGGGAGTCCGTCATGGTGTCTGCTCACACATCTGCTGGAAAAACTGTTGTTGCGTCGTACGCGATTGCGTTGTGTTTGCGTAATAAGCAACGTGTGATATACACCGCACCCATCAAAGCTCTCAGCAACCAAAAGTACAGAGAGTTCAAAGAGGAGTTTTCGGATGTTGGGTTGATGACTGGAGATGTAACAATCGACCCCAACGCTTCGTGCTTGAGCATGCAGTACAAAGGATCAGAGGTGACGCGAGAGGTGGCTTGGATAATCTTTGATGAGGTGCATTACATGAGGGATCTCGAGAGAGGTGTGGTGTGGGAAGAGAGCATTGTTATGGCTCCAAAGAATGCTCGCTTTGTCTTCCTCTCAGCAACTGTGCCCAATGCTAAGGAATTTGCTGATTGGCTCGCAAAGGTTCATCGACAGCCTTGCCACATTGTCTATACGGATTATAGGCCCACTCCTCTTCAGCATTATATTTTCCCCTCCGGAGGGGATGGTCTGTACTTGGTAGTTGATGAAAAAGGAAAGTTTCGTGAGGACAGCTTTCAACAAGCTCTTAATGCACTTGCTCCTGCCTCCGACGCTGCTAAGAAGAAGGAAAATGGAAAGTGGCAGAAGGGTCTGGTCATCGGCAAGGCCGCTGAAGAAAGCGATATCTTCAAGATGGTGAAAATGATAATTCAGCGTCAGTATGACCCTGTCATACTTTTCAGCTTTAGCAAAAGGGAATGTGAATTACTTGCAATGCAGATGGCAAAGTTGGACTTAAATGGGGACAGTGAGAAAGCAAACATAGAAACCATCTTCTGGAGTGCTATGGATATGCTTTCAGACGACGATAAGAAGTTACCCCAGGTTACCACTATGTTACCCCTTTTAATGCGTGGAATAGGTGTGCATCATTCTGGTTTGATTCCCATTCTAAAGGAAGTGATTGAAATATTATTTCAGGAAGGTCTTATCAAGTGTTTATTTGCAACAGAGACCTTCAGCATAGGACTGAACATGCCTGCGAAAACTGTTGTATTTACCAATGTCCGGAAATTTGATGGTAATAAGTTCAGATGGATAACCAGTGGTGAGTATATACAAATGAGTGGTCGTGCTGGTCGCCGGGGTATTGATAAACGTGGGATATGCATACTTATGGTAGATGAGAAGCTAGAGCCATCTACTGCCAAAATGATGCTTAAAGGAAATGCTGATAGTTTGAACAGTGCCTTTCATTTAAGCTACAACATGCTTCTTAATCAATTGCGCTCTGAAGATGGTAATCCAGAAAATTTACTTCGTAACTCATTCTATCAGTTCCAAGCTGATCGTGCCATTCCCAATCTGGAGAAACAAACAAAGGAGCTTGAGCAAGAGAGGGATTCAATTATAATTGAAGAAGAAGATAGTGTAAGGAATTATTACAATCTAGTACAGCAATACAAGAGTTTAAAGCAGGATCTTCGTGAGATTGTGATTTCTCCAAAGTACTGCTTACCATTTCTGAAACCTGGTAGGCTTGTGTCAATCCAATGCACCAAGAGTGATGAAGCTTCCGCTTCTTTTTCTACCGAGGACCATGTCACATGGGGTGTTATACTTAATTTTCAACGAGTGAAAACTGTTTCTGAAGACGATGCAGTTAAGAGACCTGAAGATTCAAACTACACAGTGGATGTTTTGACTAGGTGTCTGGTTGGCACAAATGGAGTTGCAAAAAAAACACTCAAAATTGTTCCTTTGAAAGAACTGGGAGAACCTGTTGTTGTTTCGATTTCCATCTCTCAGATTAATAGCATGAGTCGACTTTGTATGGTCATACCAAATGATCTTTTGTCATTACAAGCTCGAGAAAACACACTGAAGAAAGTTCTTGAAACTCTTTCAAGATTTGGAGAGAAGATTCCTCTTTTAGATCCTGAAGAAGACATGAAGATTCAAAGTAGGTCGTACAAAAAAGTGGCTCGTAGGATTGAGGCTTTAGAGAACCTGTTTGACAGGCATGAAATTGCAAAGTCACCACTAATTGAGCAAAAGCTCAAGGTTTTTCACATGAAGCAGGAATTGGCAGCGAAGATCAAGTCAATTAAGAAAACAATGCGCTCTTCGACTGCGTTGGCTTTTAAAGATGAACTTAAGGCACGAAAACGTGTTCTTCGGAGGCTAGGATATGTTACTAGCGATGATGTTGTGCAGTTGAAGGGTAAGGTTGCTTGTGAAATCAGTAGTGCAGATGAGTTGACCCTCACAGAGCTCATGTTCAATGGCGTTTTCAAGGACATAAAGGTAGAAGAGATGGTATCTCTTCTCTCATGTTTTGTTTGGCGGGAAAAACTAAAAGATGCTACAAAACCAAGGGAAGAACTTGACCTGCTCTTCTTGCAATTACAAGAAACTGCTCGGAGGGTGGCTGAAGTTCAGCTTGAGTGCAAGGTTGAAATTGACATCGATAGCTTTGTGAGTTCATTTCGGCCTGATATTATGGAGGCTGCATATGCTTGGGCAAAAGGCTCAAAATTTTATGAGATAATGTCAGTTACAGGGGTTTTCGAGGGTAGCTTAATCAGGGCAATCAGGAGATTAGAGGAAGTTCTTCAGCAACTTATACACGCAGCCAATTCTATTGGAGAAACTGATCTTGAATCAAAATTTGAGGAGGCTGTTTCAAAAATCAAGAGAGACATTGTCTTTGCAGCTTCCCTATACTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0022613 GO:0031123 GO:0031125 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

982

Amino Acids

111.21

Weight (kDa)

6.58

Isoelectric Point (pI)

49.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	68 - 207	3.4e-06	Type III restriction enzyme, res subunit
DEAD	PF00270	71 - 212	7.5e-22	DEAD/DEAH box helicase
DEXQc_Suv3	PF22527	92 - 194	2.8e-08	DEXQ-box helicase domain of Suv3
MTR4-like_stalk	PF21408	509 - 571	1.6e-20	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	573 - 780	9.5e-49	Mtr4-like, beta-barrel domain
DSHCT	PF08148	807 - 977	6.7e-55	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015112)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59760
fragaria_vesca	FvH4_5g28050 FvH4_5g28050 FvH4_5g28050
malus_domestica	MD08G1247600.v1.1
prunus_persica	Prupe.6G113400_v2.0.a1
pyrus_communis	pycom08g21450
rosa_chinensis	RchiOBHm_Chr7g0220221
rosa_laevigata	RLG00000002249
rosa_multiflora	Rmu_sc0001136.1_g000019
rosa_roxburghii	Rroxscaffold_3G00239700
rosa_rugosa	Rorug07G0191200
rosa_samantha	Rh7AG333300 Rh7CG350900 Rh7DG329600
rosa_wichuraiana	Rw7G028110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1628
Acc36I	ACCTGC	1 cut(s)	2616
AccB7I	CCANNNNNTGG	1 cut(s)	1053
AccI	GTMKAC	1 cut(s)	2041
AccII	CGCG	2 cut(s)	308, 493
AccIII	TCCGGA	2 cut(s)	731, 1284
AciI	CCGC	9 cut(s)	155, 158, 161, 164, 183, 351, 885, 1794, 2563
AclWI	GGATC	5 cut(s)	13, 487, 546, 1704, 2147
AcsI	RAATTY	6 cut(s)	191, 635, 1289, 1528, 2753, 2885
AdeI	CACNNNGTG	1 cut(s)	127
AfaI	GTAC	8 cut(s)	134, 305, 383, 473, 749, 1671, 1727, 2189
AfiI	CCNNNNNNNGG	4 cut(s)	734, 735, 1053, 1284
AflII	CTTAAG	1 cut(s)	2372
AflIII	ACRYGT	3 cut(s)	126, 337, 2386
AhdI	GACNNNNNGTC	1 cut(s)	1926
AjiI	CACGTC	1 cut(s)	127
AjnI	CCWGG	2 cut(s)	1098, 1747
AjuI	GAANNNNNNNTTGG	2 cut(s)	2285, 2317
Alw21I	GWGCWC	2 cut(s)	123, 2493
Alw26I	GTCTC	3 cut(s)	1223, 1879, 2910
Alw44I	GTGCAC	1 cut(s)	119
AlwI	GGATC	5 cut(s)	13, 487, 546, 1704, 2147
AlwNI	CAGNNNCTG	1 cut(s)	617
Ama87I	CYCGRG	2 cut(s)	542, 2085
Aor13HI	TCCGGA	2 cut(s)	731, 1284
AoxI	GGCC	3 cut(s)	698, 882, 2708
ApaLI	GTGCAC	1 cut(s)	119
ApeKI	GCWGC	7 cut(s)	41, 44, 830, 2306, 2726, 2849, 2930
ApoI	RAATTY	6 cut(s)	191, 635, 1289, 1528, 2753, 2885
ArsI	GACNNNNNNTTYG	6 cut(s)	1330, 1362, 2303, 2335, 2909, 2941
AseI	ATTAAT	1 cut(s)	2028
Asp700I	GAANNNNTTC	1 cut(s)	2829
AspLEI	GCGC	2 cut(s)	1506, 2340
AspS9I	GGNCC	2 cut(s)	699, 1813
AsuC2I	CCSGG	1 cut(s)	1361
AsuHPI	GGTGA	4 cut(s)	499, 925, 1337, 2247
AvaI	CYCGRG	2 cut(s)	542, 2085
AvaII	GGWCC	1 cut(s)	1813
AxyI	CCTNAGG	1 cut(s)	138
BaeGI	GKGCMC	2 cut(s)	123, 624
BamHI	GGATCC	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	2493
BbsI	GAAGAC	3 cut(s)	595, 1875, 2169
Bbv12I	GWGCWC	2 cut(s)	123, 2493
BbvI	GCAGC	7 cut(s)	53, 56, 817, 2318, 2713, 2861, 2942
BcgI	CGANNNNNNTGC	4 cut(s)	654, 688, 1682, 1716
BciT130I	CCWGG	2 cut(s)	1100, 1749
BcnI	CCSGG	1 cut(s)	1361
BcoDI	GTCTC	3 cut(s)	1223, 1879, 2910
BfaI	CTAG	6 cut(s)	59, 1409, 1667, 1923, 2402, 2415
BfmI	CTRYAG	1 cut(s)	39
BfrI	CTTAAG	1 cut(s)	2372
BfuAI	ACCTGC	1 cut(s)	2616
BmcAI	AGTACT	1 cut(s)	1727
Bme1390I	CCNGG	3 cut(s)	1100, 1361, 1749
Bme18I	GGWCC	1 cut(s)	1813
BmeRI	GACNNNNNGTC	1 cut(s)	1926
BmeT110I	CYCGRG	2 cut(s)	542, 2085
BmgBI	CACGTC	1 cut(s)	127
BmgT120I	GGNCC	2 cut(s)	699, 1813
BmiI	GGNNCC	2 cut(s)	7, 582
BmrFI	CCNGG	3 cut(s)	1100, 1361, 1749
BmrI	ACTGGG	1 cut(s)	1997
BmsI	GCATC	4 cut(s)	1153, 1422, 1864, 2569
BmuI	ACTGGG	1 cut(s)	1997
BpiI	GAAGAC	3 cut(s)	595, 1875, 2169
BpmI	CTGGAG	3 cut(s)	447, 1075, 1604
Bpu10I	CCTNAGC	1 cut(s)	630
BpuEI	CTTGAG	4 cut(s)	481, 1625, 2261, 2681
BpuMI	CCSGG	1 cut(s)	1361
Bsa29I	ATCGAT	1 cut(s)	2685
BsaBI	GATNNNNATC	2 cut(s)	1518, 2015
BsaI	GGTCTC	2 cut(s)	1223, 1879
BsaJI	CCNNGG	4 cut(s)	1098, 1360, 1809, 2591
BsaWI	WCCGGW	2 cut(s)	731, 1284
BsaXI	ACNNNNNCTCC	2 cut(s)	1983, 2013
Bsc4I	CCNNNNNNNGG	4 cut(s)	734, 735, 1053, 1284
Bse1I	ACTGG	3 cut(s)	430, 1320, 1992
Bse21I	CCTNAGG	1 cut(s)	138
Bse3DI	GCAATG	1 cut(s)	996
Bse8I	GATNNNNATC	2 cut(s)	1518, 2015
BseAI	TCCGGA	2 cut(s)	731, 1284
BseBI	CCWGG	2 cut(s)	1100, 1749
BseCI	ATCGAT	1 cut(s)	2685
BseDI	CCNNGG	4 cut(s)	1098, 1360, 1809, 2591
BseGI	GGATG	3 cut(s)	415, 745, 1918
BseJI	GATNNNNATC	2 cut(s)	1518, 2015
BseLI	CCNNNNNNNGG	4 cut(s)	734, 735, 1053, 1284
BseMI	GCAATG	1 cut(s)	996
BseMII	CTCAG	4 cut(s)	129, 382, 624, 2036
BseNI	ACTGG	3 cut(s)	430, 1320, 1992
BseRI	GAGGAG	3 cut(s)	413, 696, 2906
BseSI	GKGCMC	2 cut(s)	123, 624
BseXI	GCAGC	7 cut(s)	53, 56, 817, 2318, 2713, 2861, 2942
BsgI	GTGCAG	2 cut(s)	2450, 2487
Bsh1236I	CGCG	2 cut(s)	308, 493
BshFI	GGCC	3 cut(s)	700, 884, 2710
BshVI	ATCGAT	1 cut(s)	2685
BsiHKAI	GWGCWC	2 cut(s)	123, 2493
BsiHKCI	CYCGRG	2 cut(s)	542, 2085
BsiSI	CCGG	3 cut(s)	732, 1285, 1360
BsiWI	CGTACG	1 cut(s)	303
BslFI	GGGAC	1 cut(s)	1034
BslI	CCNNNNNNNGG	4 cut(s)	734, 735, 1053, 1284
BsmAI	GTCTC	3 cut(s)	1223, 1879, 2910
BsmFI	GGGAC	1 cut(s)	1034
BsmI	GAATGC	1 cut(s)	595
BsnI	GGCC	3 cut(s)	700, 884, 2710
Bso31I	GGTCTC	2 cut(s)	1223, 1879
BsoBI	CYCGRG	2 cut(s)	542, 2085
Bsp1286I	GDGCHC	3 cut(s)	123, 624, 2493
Bsp13I	TCCGGA	2 cut(s)	731, 1284
Bsp143I	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
BspACI	CCGC	9 cut(s)	155, 158, 161, 164, 183, 351, 885, 1794, 2563
BspANI	GGCC	3 cut(s)	700, 884, 2710
BspCNI	CTCAG	4 cut(s)	130, 381, 623, 2035
BspDI	ATCGAT	1 cut(s)	2685
BspEI	TCCGGA	2 cut(s)	731, 1284
BspFNI	CGCG	2 cut(s)	308, 493
BspLI	GGNNCC	2 cut(s)	7, 582
BspMAI	CTGCAG	1 cut(s)	43
BspMI	ACCTGC	1 cut(s)	2616
BspPI	GGATC	5 cut(s)	13, 487, 546, 1704, 2147
BspQI	GCTCTTC	2 cut(s)	2347, 2618
BspTI	CTTAAG	1 cut(s)	2372
BspTNI	GGTCTC	2 cut(s)	1223, 1879
BsrDI	GCAATG	1 cut(s)	996
BsrI	ACTGG	3 cut(s)	430, 1320, 1992
BssECI	CCNNGG	4 cut(s)	1098, 1360, 1809, 2591
BssMI	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
BssT1I	CCWWGG	1 cut(s)	2591
Bst2UI	CCWGG	2 cut(s)	1100, 1749
Bst4CI	ACNGT	7 cut(s)	292, 619, 1025, 1267, 1463, 1861, 1909
Bst6I	CTCTTC	7 cut(s)	8, 558, 714, 2347, 2523, 2547, 2618
BstAFI	CTTAAG	1 cut(s)	2372
BstC8I	GCNNGC	5 cut(s)	119, 467, 595, 651, 1257
BstDEI	CTNAG	6 cut(s)	138, 368, 610, 630, 834, 2022
BstEII	GGTNACC	1 cut(s)	1102
BstF5I	GGATG	3 cut(s)	415, 745, 1918
BstFNI	CGCG	2 cut(s)	308, 493
BstHHI	GCGC	2 cut(s)	1506, 2340
BstKTI	GATC	9 cut(s)	8, 482, 541, 1567, 1699, 2068, 2155, 2316, 2875
BstMAI	GTCTC	3 cut(s)	1223, 1879, 2910
BstMBI	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
BstMWI	GCNNNNNNNGC	8 cut(s)	163, 305, 331, 806, 827, 1568, 2617, 2732
BstNI	CCWGG	2 cut(s)	1100, 1749
BstNSI	RCATGY	3 cut(s)	469, 1255, 1489
BstPI	GGTNACC	1 cut(s)	1102
BstSCI	CCNGG	3 cut(s)	1098, 1359, 1747
BstSFI	CTRYAG	1 cut(s)	39
BstSLI	GKGCMC	2 cut(s)	123, 624
BstUI	CGCG	2 cut(s)	308, 493
BstV1I	GCAGC	7 cut(s)	53, 56, 817, 2318, 2713, 2861, 2942
BstV2I	GAAGAC	3 cut(s)	595, 1875, 2169
BstX2I	RGATCY	4 cut(s)	5, 538, 1696, 2152
BstYI	RGATCY	4 cut(s)	5, 538, 1696, 2152
Bsu15I	ATCGAT	1 cut(s)	2685
Bsu36I	CCTNAGG	1 cut(s)	138
BsuRI	GGCC	3 cut(s)	700, 884, 2710
BsuTUI	ATCGAT	1 cut(s)	2685
BtgZI	GCGATG	1 cut(s)	2433
BtrI	CACGTC	1 cut(s)	127
BtsCI	GGATG	3 cut(s)	415, 745, 1918
BtsIMutI	CAGTG	5 cut(s)	1030, 1327, 1468, 1914, 2096
BveI	ACCTGC	1 cut(s)	2616
Cac8I	GCNNGC	5 cut(s)	119, 467, 595, 651, 1257
CaiI	CAGNNNCTG	1 cut(s)	617
CfoI	GCGC	2 cut(s)	1506, 2340
Cfr13I	GGNCC	2 cut(s)	699, 1813
ClaI	ATCGAT	1 cut(s)	2685
CseI	GACGC	4 cut(s)	288, 499, 836, 920
CsiI	ACCWGGT	1 cut(s)	1747
Csp6I	GTAC	8 cut(s)	133, 304, 382, 472, 748, 1670, 1726, 2188
CviQI	GTAC	8 cut(s)	133, 304, 382, 472, 748, 1670, 1726, 2188
DdeI	CTNAG	6 cut(s)	138, 368, 610, 630, 834, 2022
DpnI	GATC	9 cut(s)	7, 481, 540, 1566, 1698, 2067, 2154, 2315, 2874
DpnII	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
DraI	TTTAAA	2 cut(s)	1689, 2362
DraIII	CACNNNGTG	1 cut(s)	127
DriI	GACNNNNNGTC	1 cut(s)	1926
Eam1104I	CTCTTC	7 cut(s)	8, 558, 714, 2347, 2523, 2547, 2618
Eam1105I	GACNNNNNGTC	1 cut(s)	1926
EarI	CTCTTC	7 cut(s)	8, 558, 714, 2347, 2523, 2547, 2618
EciI	GGCGGA	1 cut(s)	144
Ecl136II	GAGCTC	1 cut(s)	2491
Eco130I	CCWWGG	1 cut(s)	2591
Eco24I	GRGCYC	1 cut(s)	2493
Eco31I	GGTCTC	2 cut(s)	1223, 1879
Eco32I	GATATC	1 cut(s)	901
Eco47I	GGWCC	1 cut(s)	1813
Eco53kI	GAGCTC	1 cut(s)	2491
Eco81I	CCTNAGG	1 cut(s)	138
Eco88I	CYCGRG	2 cut(s)	542, 2085
Eco91I	GGTNACC	1 cut(s)	1102
EcoICRI	GAGCTC	1 cut(s)	2491
EcoO65I	GGTNACC	1 cut(s)	1102
EcoRII	CCWGG	2 cut(s)	1098, 1747
EcoRV	GATATC	1 cut(s)	901
EcoT14I	CCWWGG	1 cut(s)	2591
EcoT22I	ATGCAT	2 cut(s)	239, 1388
EcoT38I	GRGCYC	1 cut(s)	2493
ErhI	CCWWGG	1 cut(s)	2591
FalI	AAGNNNNNCTT	8 cut(s)	995, 1027, 1715, 1747, 2355, 2387, 2643, 2675
FaqI	GGGAC	1 cut(s)	1034
FauI	CCCGC	2 cut(s)	190, 2556
FauNDI	CATATG	1 cut(s)	2731
FblI	GTMKAC	1 cut(s)	2041
FokI	GGATG	3 cut(s)	422, 752, 1925
FriOI	GRGCYC	1 cut(s)	2493
FspBI	CTAG	6 cut(s)	59, 1409, 1667, 1923, 2402, 2415
GlaI	GCGC	2 cut(s)	1505, 2339
GsuI	CTGGAG	3 cut(s)	447, 1075, 1604
HaeIII	GGCC	3 cut(s)	700, 884, 2710
HapII	CCGG	3 cut(s)	732, 1285, 1360
HgaI	GACGC	4 cut(s)	288, 499, 836, 920
HhaI	GCGC	2 cut(s)	1506, 2340
Hin6I	GCGC	2 cut(s)	1504, 2338
HinP1I	GCGC	2 cut(s)	1504, 2338
HincII	GTYRAC	3 cut(s)	97, 2042, 2478
HindII	GTYRAC	3 cut(s)	97, 2042, 2478
HindIII	AAGCTT	1 cut(s)	1788
HinfI	GANTC	9 cut(s)	35, 251, 1159, 1619, 1895, 2038, 2140, 2173, 2879
HpaII	CCGG	3 cut(s)	732, 1285, 1360
HphI	GGTGA	4 cut(s)	499, 925, 1337, 2247
Hpy166II	GTNNAC	4 cut(s)	97, 121, 2042, 2478
Hpy8I	GTNNAC	4 cut(s)	97, 121, 2042, 2478
Hpy99I	CGWCG	4 cut(s)	128, 304, 830, 1085
HpyAV	CCTTC	7 cut(s)	177, 208, 835, 856, 1193, 1243, 2431
HpyCH4III	ACNGT	7 cut(s)	292, 619, 1025, 1267, 1463, 1861, 1909
HpyCH4IV	ACGT	5 cut(s)	126, 147, 337, 1375, 2386
HpyF10VI	GCNNNNNNNGC	8 cut(s)	163, 305, 331, 806, 827, 1568, 2617, 2732
HpyF3I	CTNAG	6 cut(s)	138, 368, 610, 630, 834, 2022
HpySE526I	ACGT	5 cut(s)	126, 147, 337, 1375, 2386
HspAI	GCGC	2 cut(s)	1504, 2338
Kpn2I	TCCGGA	2 cut(s)	731, 1284
Kzo9I	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
LguI	GCTCTTC	2 cut(s)	2347, 2618
LmnI	GCTCC	4 cut(s)	52, 586, 820, 1600
Lsp1109I	GCAGC	7 cut(s)	53, 56, 817, 2318, 2713, 2861, 2942
LweI	GCATC	4 cut(s)	1153, 1422, 1864, 2569
MabI	ACCWGGT	1 cut(s)	1747
MaeI	CTAG	6 cut(s)	59, 1409, 1667, 1923, 2402, 2415
MaeII	ACGT	5 cut(s)	126, 147, 337, 1375, 2386
MalI	GATC	9 cut(s)	7, 481, 540, 1566, 1698, 2067, 2154, 2315, 2874
MboI	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
MfeI	CAATTG	1 cut(s)	1499
MflI	RGATCY	4 cut(s)	5, 538, 1696, 2152
MhlI	GDGCHC	3 cut(s)	123, 624, 2493
MlyI	GAGTC	3 cut(s)	29, 260, 2047
MmeI	TCCRAC	3 cut(s)	48, 849, 987
Mph1103I	ATGCAT	2 cut(s)	239, 1388
MroI	TCCGGA	2 cut(s)	731, 1284
MroXI	GAANNNNTTC	1 cut(s)	2829
MslI	CAYNNNNRTG	2 cut(s)	575, 1391
MspA1I	CMGCKG	1 cut(s)	887
MspCI	CTTAAG	1 cut(s)	2372
MspI	CCGG	3 cut(s)	732, 1285, 1360
MspR9I	CCNGG	3 cut(s)	1100, 1361, 1749
MunI	CAATTG	1 cut(s)	1499
Mva1269I	GAATGC	1 cut(s)	595
MvaI	CCWGG	2 cut(s)	1100, 1749
MvnI	CGCG	2 cut(s)	308, 493
MwoI	GCNNNNNNNGC	8 cut(s)	163, 305, 331, 806, 827, 1568, 2617, 2732
NciI	CCSGG	1 cut(s)	1361
NdeI	CATATG	1 cut(s)	2731
NdeII	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
NlaIV	GGNNCC	2 cut(s)	7, 582
NmuCI	GTSAC	3 cut(s)	487, 1819, 2253
NsiI	ATGCAT	2 cut(s)	239, 1388
NspI	RCATGY	3 cut(s)	469, 1255, 1489
PaeI	GCATGC	1 cut(s)	469
PaeR7I	CTCGAG	2 cut(s)	542, 2085
PciSI	GCTCTTC	2 cut(s)	2347, 2618
PctI	GAATGC	1 cut(s)	595
PdmI	GAANNNNTTC	1 cut(s)	2829
PfeI	GAWTC	6 cut(s)	1159, 1619, 1895, 2140, 2173, 2879
Pfl23II	CGTACG	1 cut(s)	303
PflFI	GACNNNGTC	3 cut(s)	944, 1817, 2921
PflMI	CCANNNNNTGG	1 cut(s)	1053
PleI	GAGTC	3 cut(s)	29, 259, 2046
PpsI	GAGTC	3 cut(s)	29, 259, 2046
PshBI	ATTAAT	1 cut(s)	2028
PsiI	TTATAA	1 cut(s)	1628
Psp124BI	GAGCTC	1 cut(s)	2493
Psp6I	CCWGG	2 cut(s)	1098, 1747
PspEI	GGTNACC	1 cut(s)	1102
PspGI	CCWGG	2 cut(s)	1098, 1747
PspLI	CGTACG	1 cut(s)	303
PspN4I	GGNNCC	2 cut(s)	7, 582
PspPI	GGNCC	2 cut(s)	699, 1813
PsrI	GAACNNNNNNTAC	2 cut(s)	374, 406
PstI	CTGCAG	1 cut(s)	43
PstNI	CAGNNNCTG	1 cut(s)	617
PsuI	RGATCY	4 cut(s)	5, 538, 1696, 2152
PsyI	GACNNNGTC	3 cut(s)	944, 1817, 2921
RsaI	GTAC	8 cut(s)	134, 305, 383, 473, 749, 1671, 1727, 2189
RsaNI	GTAC	8 cut(s)	133, 304, 382, 472, 748, 1670, 1726, 2188
RseI	CAYNNNNRTG	2 cut(s)	575, 1391
SacI	GAGCTC	1 cut(s)	2493
SalI	GTCGAC	1 cut(s)	2040
SapI	GCTCTTC	2 cut(s)	2347, 2618
Sau3AI	GATC	9 cut(s)	5, 479, 538, 1564, 1696, 2065, 2152, 2313, 2872
Sau96I	GGNCC	2 cut(s)	699, 1813
ScaI	AGTACT	1 cut(s)	1727
SchI	GAGTC	3 cut(s)	29, 260, 2047
ScrFI	CCNGG	3 cut(s)	1100, 1361, 1749
SduI	GDGCHC	3 cut(s)	123, 624, 2493
SexAI	ACCWGGT	1 cut(s)	1747
SfaNI	GCATC	4 cut(s)	1153, 1422, 1864, 2569
SfcI	CTRYAG	1 cut(s)	39
Sfr274I	CTCGAG	2 cut(s)	542, 2085
SinI	GGWCC	1 cut(s)	1813
SlaI	CTCGAG	2 cut(s)	542, 2085
SmiMI	CAYNNNNRTG	2 cut(s)	575, 1391
SmlI	CTYRAG	7 cut(s)	460, 542, 1604, 2085, 2276, 2372, 2660
SmoI	CTYRAG	7 cut(s)	460, 542, 1604, 2085, 2276, 2372, 2660
SphI	GCATGC	1 cut(s)	469
SsiI	CCGC	9 cut(s)	155, 158, 161, 164, 183, 351, 885, 1794, 2563
SspI	AATATT	1 cut(s)	1188
SspMI	CTAG	6 cut(s)	59, 1409, 1667, 1923, 2402, 2415
SstI	GAGCTC	1 cut(s)	2493
StyD4I	CCNGG	3 cut(s)	1098, 1359, 1747
StyI	CCWWGG	1 cut(s)	2591
TaaI	ACNGT	7 cut(s)	292, 619, 1025, 1267, 1463, 1861, 1909
TaiI	ACGT	5 cut(s)	129, 150, 340, 1378, 2389
TatI	WGTACW	5 cut(s)	381, 471, 747, 1669, 1725
TauI	GCSGC	4 cut(s)	160, 163, 166, 887
TfiI	GAWTC	6 cut(s)	1159, 1619, 1895, 2140, 2173, 2879
TscAI	CASTG	5 cut(s)	1030, 1327, 1468, 1914, 2103
TseFI	GTSAC	3 cut(s)	487, 1819, 2253
TseI	GCWGC	7 cut(s)	41, 44, 830, 2306, 2726, 2849, 2930
Tsp45I	GTSAC	3 cut(s)	487, 1819, 2253
TspGWI	ACGGA	2 cut(s)	244, 704
TspRI	CASTG	5 cut(s)	1030, 1327, 1468, 1914, 2103
Tth111I	GACNNNGTC	3 cut(s)	944, 1817, 2921
Van91I	CCANNNNNTGG	1 cut(s)	1053
Vha464I	CTTAAG	1 cut(s)	2372
VneI	GTGCAC	1 cut(s)	119
VpaK11BI	GGWCC	1 cut(s)	1813
VspI	ATTAAT	1 cut(s)	2028
XapI	RAATTY	6 cut(s)	191, 635, 1289, 1528, 2753, 2885
XceI	RCATGY	3 cut(s)	469, 1255, 1489
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1579
XhoI	CTCGAG	2 cut(s)	542, 2085
XmiI	GTMKAC	1 cut(s)	2041
XmnI	GAANNNNTTC	1 cut(s)	2829
XspI	CTAG	6 cut(s)	59, 1409, 1667, 1923, 2402, 2415
ZrmI	AGTACT	1 cut(s)	1727
Zsp2I	ATGCAT	2 cut(s)	239, 1388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.