FvH4_5g28110

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013922
Physical Location & Seq
Reverse (-)
19323853 .. 19324764
912 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28110.t1

Sequence Viewer

Length: 912 bp
ATGAGTCGATCACCAAAAACCCATCCAAGTTTCAACTATAAGCTTCTTCTCCTCGGAGTTTTCCTGGCTTTCGTTGTTCTCTTTGTCTTGAGATCAAGCTTCTCGTCATCATCTGATTCCAGTGAAAACACATCTACCATCTCACCAGAGGTACCTTTATTTAGTCGTTCGGCTAAAGATTCAAGTAGTAGCTCTACAAACTGCTCACCAAGTAGTACTACTGGTTGTAGCAAGATCCCATCCTCTCTAGCTCAAACCATAATCCACTACACAACCTCAACCATCACCCCACAACAAACCCTCAAGGAAATCTCAGTGACAGCAAAGATACTAGACAAGAAATCACCATGCAACTTCCTTGTGTTTGGCCTCGGCCACGACAGCCTTATGTGGAGTGCACTCAACCATGGGGGCAGGACAATTTTCCTGGAAGAAGACGAGTCCTGGATCGAGCAAATCCGACGTCGCTTCCCCACATTGGAGTCATACCATGTAACATATAACAGCAAGGTAAATGAGGCTGATAATCTCATGGATGTTGGCCAGGGGCCTGAGTGTACTGCAGTTGGTAGTGACATCAAGTACAGCATGTGCCAATTGGCTTTGAAGGGTCTGCCGAGTCAAGTGTATGACATGAAATGGGATTTGATAATGGTGGATGCACCCACCGGGTATCACGACCAAGCACCGGGGAGAATGAGTGCCATATACACAGCCGGAATGATTGCCAGAAATAAAGAGGAAGGGGAGACTACTCATGTGTTTGTTCATGATGTGAATAGAGTTGTGGAAGACAAGTTCTCTATGTCATTCCTTTGTAAAGGTTACATGCAGAAACAGGAAGGGAGGTTGAGGCACTTCACTATTCCTAGTCACATGGATGGTTCCAGCAGGCCATTTTGTCCTGAATGA

Protein Analysis

304

Amino Acids

33.7

Weight (kDa)

6.55

Isoelectric Point (pI)

62.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 102 - 287 7.6e-80 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013922)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09610
fragaria_vesca FvH4_5g28110
malus_domestica MD08G1244700.v1.1 MD15G1439800.v1.1
prunus_persica Prupe.1G582100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0271041
rosa_laevigata RLG00000002227
rosa_multiflora Rmu_co8384627.1_g000001
rosa_roxburghii Rroxscaffold_7G00197370
rosa_rugosa Rorug06G0058100 Rorug07G0193100
rosa_samantha Rh6AG177900 Rh6BG181200 Rh6CG177000 Rh6DG169200 Rh7BG325400
rosa_wichuraiana Rw6G015180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 466
Acc65I GGTACC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 151
AclWI GGATC 2 cut(s) 229, 455
AcoI YGGCCR 2 cut(s) 373, 541
AcyI GRCGYC 1 cut(s) 463
AfaI GTAC 4 cut(s) 153, 217, 559, 584
AfiI CCNNNNNNNGG 2 cut(s) 478, 688
AgsI TTSAA 3 cut(s) 34, 183, 607
AjnI CCWGG 4 cut(s) 63, 426, 443, 543
AloI GAACNNNNNNTCC 2 cut(s) 782, 814
AluBI AGCT 4 cut(s) 43, 99, 192, 251
AluI AGCT 4 cut(s) 43, 99, 192, 251
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 743
Alw44I GTGCAC 1 cut(s) 396
AlwI GGATC 2 cut(s) 229, 455
AoxI GGCC 5 cut(s) 367, 373, 541, 548, 893
ApaLI GTGCAC 1 cut(s) 396
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
Asp718I GGTACC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 548
AsuC2I CCSGG 2 cut(s) 670, 690
AsuHPI GGTGA 5 cut(s) 3, 135, 198, 277, 336
BaeGI GKGCMC 1 cut(s) 400
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 441, 798
Bbv12I GWGCWC 1 cut(s) 400
BccI CCATC 5 cut(s) 30, 146, 247, 290, 875
BciT130I CCWGG 4 cut(s) 65, 428, 445, 545
BcnI CCSGG 2 cut(s) 670, 690
BcoDI GTCTC 1 cut(s) 743
BfaI CTAG 3 cut(s) 248, 332, 870
BfmI CTRYAG 1 cut(s) 561
BmcAI AGTACT 1 cut(s) 217
Bme1390I CCNGG 6 cut(s) 65, 428, 445, 545, 670, 690
BmgT120I GGNCC 1 cut(s) 548
BmiI GGNNCC 3 cut(s) 153, 549, 886
BmrFI CCNGG 6 cut(s) 65, 428, 445, 545, 670, 690
BmsI GCATC 1 cut(s) 649
BpiI GAAGAC 2 cut(s) 441, 798
BpuEI CTTGAG 2 cut(s) 109, 287
BpuMI CCSGG 2 cut(s) 670, 690
BsaHI GRCGYC 1 cut(s) 463
BsaJI CCNNGG 5 cut(s) 52, 370, 406, 544, 689
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 688
Bse1I ACTGG 2 cut(s) 120, 226
BseBI CCWGG 4 cut(s) 65, 428, 445, 545
BseDI CCNNGG 5 cut(s) 52, 370, 406, 544, 689
BseGI GGATG 5 cut(s) 22, 239, 541, 664, 886
BseLI CCNNNNNNNGG 2 cut(s) 478, 688
BseMII CTCAG 2 cut(s) 327, 543
BseNI ACTGG 2 cut(s) 120, 226
BseRI GAGGAG 1 cut(s) 41
BseSI GKGCMC 1 cut(s) 400
BshFI GGCC 5 cut(s) 369, 375, 543, 550, 895
BshNI GGYRCC 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 3 cut(s) 669, 689, 717
BslI CCNNNNNNNGG 2 cut(s) 478, 688
BsmAI GTCTC 1 cut(s) 743
BsnI GGCC 5 cut(s) 369, 375, 543, 550, 895
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 4 cut(s) 8, 92, 234, 447
Bsp19I CCATGG 1 cut(s) 406
BspANI GGCC 5 cut(s) 369, 375, 543, 550, 895
BspCNI CTCAG 2 cut(s) 326, 544
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 3 cut(s) 153, 549, 886
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 2 cut(s) 229, 455
BspT107I GGYRCC 1 cut(s) 151
BsrI ACTGG 2 cut(s) 120, 226
BssECI CCNNGG 5 cut(s) 52, 370, 406, 544, 689
BssMI GATC 4 cut(s) 8, 92, 234, 447
BssNI GRCGYC 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 4 cut(s) 65, 428, 445, 545
BstACI GRCGYC 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 893
BstDEI CTNAG 2 cut(s) 313, 552
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 5 cut(s) 22, 239, 541, 664, 886
BstKTI GATC 4 cut(s) 11, 95, 237, 450
BstMAI GTCTC 1 cut(s) 743
BstMBI GATC 4 cut(s) 8, 92, 234, 447
BstMWI GCNNNNNNNGC 1 cut(s) 381
BstNI CCWGG 4 cut(s) 65, 428, 445, 545
BstNSI RCATGY 2 cut(s) 592, 832
BstSCI CCNGG 6 cut(s) 63, 426, 443, 543, 668, 688
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 400
BstV2I GAAGAC 2 cut(s) 441, 798
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 5 cut(s) 369, 375, 543, 550, 895
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 5 cut(s) 22, 239, 541, 664, 886
BtsIMutI CAGTG 2 cut(s) 127, 321
Cac8I GCNNGC 1 cut(s) 893
CciI TCATGA 1 cut(s) 769
Cfr13I GGNCC 1 cut(s) 548
Csp6I GTAC 4 cut(s) 152, 216, 558, 583
CviQI GTAC 4 cut(s) 152, 216, 558, 583
DdeI CTNAG 2 cut(s) 313, 552
DpnI GATC 4 cut(s) 10, 94, 236, 449
DpnII GATC 4 cut(s) 8, 92, 234, 447
EaeI YGGCCR 2 cut(s) 373, 541
Eco130I CCWWGG 1 cut(s) 406
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 4 cut(s) 63, 426, 443, 543
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
FokI GGATG 5 cut(s) 9, 226, 548, 671, 893
FspBI CTAG 3 cut(s) 248, 332, 870
HaeIII GGCC 5 cut(s) 369, 375, 543, 550, 895
HapII CCGG 3 cut(s) 669, 689, 717
Hin1I GRCGYC 1 cut(s) 463
HindIII AAGCTT 2 cut(s) 41, 97
HinfI GANTC 6 cut(s) 4, 116, 179, 440, 482, 619
HpaII CCGG 3 cut(s) 669, 689, 717
HphI GGTGA 5 cut(s) 3, 135, 198, 277, 336
Hpy166II GTNNAC 2 cut(s) 398, 558
Hpy188I TCNGA 3 cut(s) 56, 115, 461
Hpy188III TCNNGA 4 cut(s) 88, 677, 770, 905
Hpy8I GTNNAC 2 cut(s) 398, 558
Hpy99I CGWCG 2 cut(s) 465, 468
HpyAV CCTTC 3 cut(s) 601, 737, 836
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 5 cut(s) 351, 398, 563, 662, 832
HpyF10VI GCNNNNNNNGC 1 cut(s) 381
HpyF3I CTNAG 2 cut(s) 313, 552
HpySE526I ACGT 1 cut(s) 463
Hsp92I GRCGYC 1 cut(s) 463
KpnI GGTACC 1 cut(s) 155
Kzo9I GATC 4 cut(s) 8, 92, 234, 447
LweI GCATC 1 cut(s) 649
MaeI CTAG 3 cut(s) 248, 332, 870
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 5 cut(s) 316, 493, 572, 824, 872
MalI GATC 4 cut(s) 10, 94, 236, 449
MboI GATC 4 cut(s) 8, 92, 234, 447
MboII GAAGA 4 cut(s) 38, 443, 446, 803
MfeI CAATTG 1 cut(s) 596
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 400
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 2 cut(s) 420, 596
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 4 cut(s) 13, 449, 491, 628
MmeI TCCRAC 1 cut(s) 484
Mox20I TGGCCA 1 cut(s) 543
MscI TGGCCA 1 cut(s) 543
MslI CAYNNNNRTG 1 cut(s) 879
Msp20I TGGCCA 1 cut(s) 543
MspI CCGG 3 cut(s) 669, 689, 717
MspR9I CCNGG 6 cut(s) 65, 428, 445, 545, 670, 690
MunI CAATTG 1 cut(s) 596
MvaI CCWGG 4 cut(s) 65, 428, 445, 545
MwoI GCNNNNNNNGC 1 cut(s) 381
NciI CCSGG 2 cut(s) 670, 690
NcoI CCATGG 1 cut(s) 406
NdeII GATC 4 cut(s) 8, 92, 234, 447
NlaIV GGNNCC 3 cut(s) 153, 549, 886
NmeAIII GCCGAG 2 cut(s) 351, 642
NmuCI GTSAC 3 cut(s) 316, 572, 872
NspI RCATGY 2 cut(s) 592, 832
PagI TCATGA 1 cut(s) 769
PfeI GAWTC 2 cut(s) 116, 179
PfoI TCCNGGA 2 cut(s) 426, 443
PleI GAGTC 4 cut(s) 12, 448, 490, 627
PpsI GAGTC 4 cut(s) 12, 448, 490, 627
Psp6I CCWGG 4 cut(s) 63, 426, 443, 543
PspGI CCWGG 4 cut(s) 63, 426, 443, 543
PspN4I GGNNCC 3 cut(s) 153, 549, 886
PspPI GGNCC 1 cut(s) 548
PstI CTGCAG 1 cut(s) 565
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 4 cut(s) 153, 217, 559, 584
RsaNI GTAC 4 cut(s) 152, 216, 558, 583
RseI CAYNNNNRTG 1 cut(s) 879
Sau3AI GATC 4 cut(s) 8, 92, 234, 447
Sau96I GGNCC 1 cut(s) 548
ScaI AGTACT 1 cut(s) 217
SchI GAGTC 4 cut(s) 13, 449, 491, 628
ScrFI CCNGG 6 cut(s) 65, 428, 445, 545, 670, 690
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 1 cut(s) 649
SfcI CTRYAG 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 879
SmlI CTYRAG 2 cut(s) 88, 302
SmoI CTYRAG 2 cut(s) 88, 302
Sse9I AATT 2 cut(s) 420, 596
SspMI CTAG 3 cut(s) 248, 332, 870
StyD4I CCNGG 6 cut(s) 63, 426, 443, 543, 668, 688
StyI CCWWGG 1 cut(s) 406
TaiI ACGT 1 cut(s) 466
TaqI TCGA 2 cut(s) 7, 450
TasI AATT 2 cut(s) 420, 596
TatI WGTACW 3 cut(s) 215, 557, 582
TfiI GAWTC 2 cut(s) 116, 179
TscAI CASTG 2 cut(s) 127, 321
TseFI GTSAC 3 cut(s) 316, 572, 872
Tsp45I GTSAC 3 cut(s) 316, 572, 872
TspDTI ATGAA 2 cut(s) 650, 758
TspRI CASTG 2 cut(s) 127, 321
VneI GTGCAC 1 cut(s) 396
XceI RCATGY 2 cut(s) 592, 832
XspI CTAG 3 cut(s) 248, 332, 870
ZraI GACGTC 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.