Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0005165

Physical Location & Seq

Genomic Coordinates Reverse (-)

16426106 .. 16430053

Gene Structure 3948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0193100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGCCGACGAAGCCAAAGCCAAGGGCAACGCCGCCTACTCCGCCGGCGACTACACCGCCGCCATTACCCACTTCACCGAAGCCATCAACCTCGCTCCCACCAACCACGTCCTCTACTCCAACCGATCCGCCTCCTACGCCAGCCTCCACAAATACTCCGACGCCCTCTCCGACGCCAAGAAGACCGTCGAGCTCAAGCCCGACTGGGCCAAAGGCTACAGCCGCCTCGGCTCCGCCCACCACGGCCTCGGCCACTTCGACGACGCCGTTTCCGCTTACAAGAAGGGCCTCGAGATTGATCCCAACAACGCCGCCCTTAAAGAGGGCTTGGCCGAGAGTCAGTCCTCCGCCGCCCGCGCCGCCCGAGCACGAGCCCCGCCGCCGACGAACCTCTTCGGCGACGCGTTCTCGGGTCCTCAGATGTGGACCAAGCTCACGGCCGACCCGAGTACCCGGGCCTTTATGCAGCAGCCGGATTTCGTTAACATGATGCAGGAGATTCAGAAAAACCCTAGCAATCTCAATCTTTATTTGAAGGACCAGAGGGTTATGCAAGCCCTAGGGGTTTTGCTCAATGTGAAGCTCCAGGGAGGCGCGTCGGAGGACGACGATATGCCGGATTTTCCGTCGGAGAGGGCCGCTGCTGAGCCGCAGAAGAAGGAGGAGAAGAAGCCGGAACCGGAGCCGGTGGCGGTGCCGGAGCCGATGGAAGTGCCGGAGGAGGAGAAGGAGGCCAGGAAGAGGAAAGCTGAGGCTTTAAAGGAGAAGGAGGCTGGCAATGCTGCTTATAAGAAGAAGGATTTCGACACTGCTATTCAGCACTATACCAAGGCAATGGAGATAGATGATGAAGATATTTCCTTCTTGTTGAATCGTGCTGCGACTTATTTGGAAATGGGTCAGTATGATGAATGCATTAAAGACTGTGACAAGGCTGTGGAAAGGGGAAGAGAGGTAAGGGCGGACTTTAAGATGATAGCAAAAGCTTTGACTAGGAAGGGAACTGCAATTGTGAAAACAGCCAAAACCTCGAAAGACTATGAGCCTGCTATTGAGATATTCCAGAAAGCTCTCACAGAGCATCGCAACCCCGACACCCTAAAGAAATTGAATGATGCTGAGAAAGCTAAGAAGGACCTAGAGCAACAAGAGTATTTTGATCCAAAGCTAGCAGATGAGGAGCGAGAGAAAGGCAACGAATTCTTCAAGCAGCAGAAGTACCCAGAGGCTATTAGGCATTACACTGAATCTTTGAGAAGAAATCCAGAGGATCCAAAGGCATATAGCAATAGAGCTGCTTGCTACACAAAGTTGGGGGCAATGCCAGAGGGATTGAAGGATGCTGAGAAGTGCATTGAGCTTGACCCAACCTTTTCAAAGGGTTATACCAGAAAAGGTGCGGTCCAGTTTTTCATGAGAGAGTATGAAAAAGCTTTGGAAACGTATCAAGAAGGTCTGAAGCATGATCCTGGCAACCAGGACTTGCTTGATGGTGTGAGGAGATGTGTAGAGCAGATTAACAAAGCTAGCCGTGGGGATCTGAGTGCCGATGAATTGAAGGAGAGACAGACTAAGGGAATGCAGGACCCAGAAATTCAGAATATCCTCTCAGATCCTGTTATGAGACAGGTGTTGGTTGATTTCCAGGAAAATCCCAAAGCTGCTCAGGAACACTCAAAGAACCCGATGGTGATGGCCAAAATCCAGAAGCTGGTCAGTGCGGGAATTGTCCAGATTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016740 GO:0016741 GO:0030775 GO:0032259 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0071554 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Polysacc_synt_4

Protein Analysis

580

Amino Acids

65.11

Weight (kDa)

5.93

Isoelectric Point (pI)

35.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_BSK1_C	PF25575	4 - 71	7.8e-07	Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1	PF00515	70 - 103	1.3e-07	Tetratricopeptide repeat
TPR_2	PF07719	70 - 103	1.9e-06	Tetratricopeptide repeat
STI1-HOP_DP	PF17830	139 - 191	6.8e-22	STI1/HOP, DP domain
TPR_BSK1_C	PF25575	249 - 318	4.9e-08	Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_11	PF13414	259 - 300	1.1e-06	TPR repeat
TPR_1	PF00515	289 - 314	4.1e-06	Tetratricopeptide repeat
TPR_12	PF13424	289 - 358	6.1e-07	Tetratricopeptide repeat
TPR_16	PF13432	397 - 448	8.2e-07	Tetratricopeptide repeat
TPR_11	PF13414	398 - 439	1.3e-08	TPR repeat
TPR_1	PF00515	425 - 458	1.2e-06	Tetratricopeptide repeat
TPR_1	PF00515	460 - 492	5.3e-06	Tetratricopeptide repeat
STI1-HOP_DP	PF17830	521 - 572	3.7e-15	STI1/HOP, DP domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26750
fragaria_vesca	FvH4_1g30250 FvH4_1g30250 FvH4_1g30250 FvH4_1g30250
malus_domestica	MD01G1063000.v1.1 MD07G1136300.v1.1
prunus_persica	Prupe.2G171300_v2.0.a1 Prupe.2G171300_v2.0.a1 Prupe.2G171300_v2.0.a1
pyrus_communis	pycom01g09340 pycom07g13360
rosa_chinensis	RchiOBHm_Chr6g0271051 RchiOBHm_Chr7g0220351
rosa_laevigata	RLG00000002228
rosa_multiflora	Rmu_sc0001136.1_g000086 Rmu_sc0001565.1_g000063
rosa_roxburghii	Rroxscaffold_3G00239510
rosa_rugosa	Rorug06G0058100 Rorug07G0193100
rosa_samantha	Rh6AG178100 Rh6BG181300 Rh6CG177100 Rh6DG169300 Rh7AG334400 Rh7BG325300 Rh7CG352100 Rh7DG331100
rosa_wichuraiana	Rw0G009890 Rw7G028270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	789
AccB1I	GGYRCC	1 cut(s)	694
AccB7I	CCANNNNNTGG	1 cut(s)	1711
AccII	CGCG	3 cut(s)	357, 404, 596
AclWI	GGATC	8 cut(s)	120, 293, 1154, 1265, 1278, 1460, 1545, 1607
AcoI	YGGCCR	5 cut(s)	3, 250, 330, 438, 1695
AcsI	RAATTY	2 cut(s)	1199, 1593
AcuI	CTGAAG	1 cut(s)	1478
AcyI	GRCGYC	3 cut(s)	162, 174, 264
AfaI	GTAC	2 cut(s)	451, 1220
AfiI	CCNNNNNNNGG	3 cut(s)	322, 741, 1711
AflIII	ACRYGT	1 cut(s)	402
AgsI	TTSAA	7 cut(s)	535, 871, 1111, 1207, 1336, 1377, 1558
AjiI	CACGTC	1 cut(s)	109
AjnI	CCWGG	5 cut(s)	585, 734, 1468, 1476, 1644
Alw21I	GWGCWC	2 cut(s)	195, 370
Alw26I	GTCTC	2 cut(s)	1558, 1618
AlwI	GGATC	8 cut(s)	120, 293, 1154, 1265, 1278, 1460, 1545, 1607
AlwNI	CAGNNNCTG	2 cut(s)	1616, 1711
Ama87I	CYCGRG	5 cut(s)	290, 363, 409, 445, 453
ApeKI	GCWGC	8 cut(s)	466, 469, 641, 782, 878, 1210, 1295, 1661
ApoI	RAATTY	2 cut(s)	1199, 1593
Asp700I	GAANNNNTTC	2 cut(s)	392, 800
AspA2I	CCTAGG	1 cut(s)	559
AspLEI	GCGC	2 cut(s)	359, 596
AsuC2I	CCSGG	2 cut(s)	454, 455
AsuHPI	GGTGA	2 cut(s)	67, 1702
AsuNHI	GCTAGC	2 cut(s)	1168, 1526
AvaI	CYCGRG	5 cut(s)	290, 363, 409, 445, 453
AvaII	GGWCC	6 cut(s)	413, 426, 538, 1135, 1402, 1585
AvrII	CCTAGG	1 cut(s)	559
BaeI	ACNNNNGTAYC	2 cut(s)	433, 466
BalI	TGGCCA	1 cut(s)	1697
BamHI	GGATCC	1 cut(s)	1270
BanI	GGYRCC	1 cut(s)	694
BanII	GRGCYC	2 cut(s)	195, 376
BauI	CACGAG	1 cut(s)	369
BbsI	GAAGAC	1 cut(s)	188
Bbv12I	GWGCWC	2 cut(s)	195, 370
BbvCI	CCTCAGC	1 cut(s)	750
BbvI	GCAGC	8 cut(s)	478, 481, 628, 769, 865, 1222, 1282, 1648
BccI	CCATC	5 cut(s)	92, 700, 1484, 1681, 1687
BceAI	ACGGC	4 cut(s)	251, 259, 453, 1515
BcgI	CGANNNNNNTGC	2 cut(s)	694, 728
BciT130I	CCWGG	5 cut(s)	587, 736, 1470, 1478, 1646
BcnI	CCSGG	2 cut(s)	454, 455
BcoDI	GTCTC	2 cut(s)	1558, 1618
BfaI	CTAG	6 cut(s)	513, 560, 993, 1139, 1169, 1527
BfmI	CTRYAG	1 cut(s)	217
BglI	GCCNNNNNGGC	1 cut(s)	228
BlnI	CCTAGG	1 cut(s)	559
BlpI	GCTNAGC	1 cut(s)	645
Bme1390I	CCNGG	7 cut(s)	454, 455, 587, 736, 1470, 1478, 1646
Bme18I	GGWCC	6 cut(s)	413, 426, 538, 1135, 1402, 1585
BmeT110I	CYCGRG	5 cut(s)	290, 363, 409, 445, 453
BmgBI	CACGTC	1 cut(s)	109
BmiI	GGNNCC	8 cut(s)	232, 414, 678, 684, 696, 702, 1272, 1587
BmrFI	CCNGG	7 cut(s)	454, 455, 587, 736, 1470, 1478, 1646
BmrI	ACTGGG	1 cut(s)	214
BmsI	GCATC	4 cut(s)	480, 1090, 1105, 1330
BmtI	GCTAGC	2 cut(s)	1172, 1530
BmuI	ACTGGG	1 cut(s)	214
BpiI	GAAGAC	1 cut(s)	188
BpmI	CTGGAG	1 cut(s)	569
Bpu10I	CCTNAGC	2 cut(s)	750, 1665
Bpu1102I	GCTNAGC	1 cut(s)	645
BpuEI	CTTGAG	1 cut(s)	179
BpuMI	CCSGG	2 cut(s)	454, 455
BsaHI	GRCGYC	3 cut(s)	162, 174, 264
BsaJI	CCNNGG	9 cut(s)	21, 226, 241, 247, 453, 559, 586, 828, 1531
BsaWI	WCCGGW	1 cut(s)	679
BsaXI	ACNNNNNCTCC	4 cut(s)	140, 152, 170, 182
Bsc4I	CCNNNNNNNGG	3 cut(s)	322, 741, 1711
Bse118I	RCCGGY	2 cut(s)	44, 685
Bse1I	ACTGG	2 cut(s)	209, 1405
Bse3DI	GCAATG	3 cut(s)	784, 840, 1326
BseBI	CCWGG	5 cut(s)	587, 736, 1470, 1478, 1646
BseDI	CCNNGG	9 cut(s)	21, 226, 241, 247, 453, 559, 586, 828, 1531
BseGI	GGATG	1 cut(s)	1345
BseLI	CCNNNNNNNGG	3 cut(s)	322, 741, 1711
BseMI	GCAATG	3 cut(s)	784, 840, 1326
BseMII	CTCAG	8 cut(s)	431, 636, 741, 1110, 1335, 1532, 1623, 1679
BseNI	ACTGG	2 cut(s)	209, 1405
BseRI	GAGGAG	5 cut(s)	677, 734, 737, 1193, 1513
BseX3I	CGGCCG	1 cut(s)	438
BseXI	GCAGC	8 cut(s)	478, 481, 628, 769, 865, 1222, 1282, 1648
Bsh1236I	CGCG	3 cut(s)	357, 404, 596
Bsh1285I	CGRYCG	1 cut(s)	441
BshNI	GGYRCC	1 cut(s)	694
BsiEI	CGRYCG	1 cut(s)	441
BsiHKAI	GWGCWC	2 cut(s)	195, 370
BsiHKCI	CYCGRG	5 cut(s)	290, 363, 409, 445, 453
BsiSI	CCGG	9 cut(s)	45, 454, 473, 617, 674, 680, 686, 698, 716
BslI	CCNNNNNNNGG	3 cut(s)	322, 741, 1711
BsmAI	GTCTC	2 cut(s)	1558, 1618
BsmI	GAATGC	2 cut(s)	917, 1584
BsoBI	CYCGRG	5 cut(s)	290, 363, 409, 445, 453
Bsp1286I	GDGCHC	3 cut(s)	195, 370, 376
Bsp143I	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
Bsp1720I	GCTNAGC	1 cut(s)	645
BspCNI	CTCAG	8 cut(s)	430, 637, 742, 1111, 1336, 1533, 1622, 1678
BspFNI	CGCG	3 cut(s)	357, 404, 596
BspHI	TCATGA	1 cut(s)	1413
BspLI	GGNNCC	8 cut(s)	232, 414, 678, 684, 696, 702, 1272, 1587
BspOI	GCTAGC	2 cut(s)	1172, 1530
BspPI	GGATC	8 cut(s)	120, 293, 1154, 1265, 1278, 1460, 1545, 1607
BspT107I	GGYRCC	1 cut(s)	694
BsrDI	GCAATG	3 cut(s)	784, 840, 1326
BsrFI	RCCGGY	2 cut(s)	44, 685
BsrI	ACTGG	2 cut(s)	209, 1405
BssAI	RCCGGY	2 cut(s)	44, 685
BssECI	CCNNGG	9 cut(s)	21, 226, 241, 247, 453, 559, 586, 828, 1531
BssMI	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
BssNI	GRCGYC	3 cut(s)	162, 174, 264
BssSI	CACGAG	1 cut(s)	369
BssT1I	CCWWGG	3 cut(s)	21, 559, 828
Bst2BI	CACGAG	1 cut(s)	369
Bst2UI	CCWGG	5 cut(s)	587, 736, 1470, 1478, 1646
Bst4CI	ACNGT	2 cut(s)	187, 926
Bst6I	CTCTTC	3 cut(s)	398, 734, 943
BstACI	GRCGYC	3 cut(s)	162, 174, 264
BstC8I	GCNNGC	9 cut(s)	46, 142, 355, 555, 775, 1047, 1170, 1300, 1528
BstDSI	CCRYGG	2 cut(s)	241, 1531
BstENI	CCTNNNNNAGG	1 cut(s)	320
BstF5I	GGATG	1 cut(s)	1345
BstFNI	CGCG	3 cut(s)	357, 404, 596
BstHHI	GCGC	2 cut(s)	359, 596
BstKTI	GATC	7 cut(s)	128, 301, 1162, 1273, 1468, 1540, 1615
BstMAI	GTCTC	2 cut(s)	1558, 1618
BstMBI	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
BstMCI	CGRYCG	1 cut(s)	441
BstNI	CCWGG	5 cut(s)	587, 736, 1470, 1478, 1646
BstSCI	CCNGG	7 cut(s)	452, 453, 585, 734, 1468, 1476, 1644
BstSFI	CTRYAG	1 cut(s)	217
BstUI	CGCG	3 cut(s)	357, 404, 596
BstV1I	GCAGC	8 cut(s)	478, 481, 628, 769, 865, 1222, 1282, 1648
BstV2I	GAAGAC	1 cut(s)	188
BstX2I	RGATCY	3 cut(s)	1270, 1537, 1612
BstXI	CCANNNNNNTGG	1 cut(s)	835
BstYI	RGATCY	3 cut(s)	1270, 1537, 1612
BstZI	CGGCCG	1 cut(s)	438
BtgI	CCRYGG	2 cut(s)	241, 1531
BtgZI	GCGATG	1 cut(s)	1067
BtrI	CACGTC	1 cut(s)	109
BtsCI	GGATG	1 cut(s)	1345
BtsI	GCAGTG	1 cut(s)	807
BtsIMutI	CAGTG	3 cut(s)	807, 1242, 1723
Cac8I	GCNNGC	9 cut(s)	46, 142, 355, 555, 775, 1047, 1170, 1300, 1528
CaiI	CAGNNNCTG	2 cut(s)	1616, 1711
CciI	TCATGA	1 cut(s)	1413
CfoI	GCGC	2 cut(s)	359, 596
Cfr10I	RCCGGY	2 cut(s)	44, 685
Cfr9I	CCCGGG	1 cut(s)	453
CseI	GACGC	5 cut(s)	170, 182, 272, 410, 585
Csp6I	GTAC	2 cut(s)	450, 1219
CviAII	CATG	3 cut(s)	487, 1414, 1463
CviQI	GTAC	2 cut(s)	450, 1219
DpnI	GATC	7 cut(s)	127, 300, 1161, 1272, 1467, 1539, 1614
DpnII	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
DraI	TTTAAA	1 cut(s)	759
EaeI	YGGCCR	5 cut(s)	3, 250, 330, 438, 1695
EagI	CGGCCG	1 cut(s)	438
Eam1104I	CTCTTC	3 cut(s)	398, 734, 943
EarI	CTCTTC	3 cut(s)	398, 734, 943
EciI	GGCGGA	5 cut(s)	31, 118, 223, 337, 977
Ecl136II	GAGCTC	1 cut(s)	193
EclXI	CGGCCG	1 cut(s)	438
Eco130I	CCWWGG	3 cut(s)	21, 559, 828
Eco24I	GRGCYC	2 cut(s)	195, 376
Eco47I	GGWCC	6 cut(s)	413, 426, 538, 1135, 1402, 1585
Eco52I	CGGCCG	1 cut(s)	438
Eco53kI	GAGCTC	1 cut(s)	193
Eco57I	CTGAAG	1 cut(s)	1478
Eco88I	CYCGRG	5 cut(s)	290, 363, 409, 445, 453
EcoICRI	GAGCTC	1 cut(s)	193
EcoNI	CCTNNNNNAGG	1 cut(s)	320
EcoO109I	RGGNCCY	4 cut(s)	286, 413, 1135, 1585
EcoRI	GAATTC	1 cut(s)	1199
EcoRII	CCWGG	5 cut(s)	585, 734, 1468, 1476, 1644
EcoT14I	CCWWGG	3 cut(s)	21, 559, 828
EcoT22I	ATGCAT	1 cut(s)	917
EcoT38I	GRGCYC	2 cut(s)	195, 376
ErhI	CCWWGG	3 cut(s)	21, 559, 828
FaeI	CATG	3 cut(s)	490, 1417, 1466
FatI	CATG	3 cut(s)	486, 1413, 1462
FauI	CCCGC	3 cut(s)	362, 384, 1714
FokI	GGATG	1 cut(s)	1352
FriOI	GRGCYC	2 cut(s)	195, 376
FspBI	CTAG	6 cut(s)	513, 560, 993, 1139, 1169, 1527
GlaI	GCGC	2 cut(s)	358, 595
GsuI	CTGGAG	1 cut(s)	569
HapII	CCGG	9 cut(s)	45, 454, 473, 617, 674, 680, 686, 698, 716
HgaI	GACGC	5 cut(s)	170, 182, 272, 410, 585
HhaI	GCGC	2 cut(s)	359, 596
Hin1I	GRCGYC	3 cut(s)	162, 174, 264
Hin1II	CATG	3 cut(s)	490, 1417, 1466
Hin6I	GCGC	2 cut(s)	357, 594
HinP1I	GCGC	2 cut(s)	357, 594
HincII	GTYRAC	1 cut(s)	484
HindII	GTYRAC	1 cut(s)	484
HindIII	AAGCTT	2 cut(s)	984, 1431
HinfI	GANTC	4 cut(s)	337, 499, 871, 1247
HpaI	GTTAAC	1 cut(s)	484
HpaII	CCGG	9 cut(s)	45, 454, 473, 617, 674, 680, 686, 698, 716
HphI	GGTGA	2 cut(s)	67, 1702
Hpy166II	GTNNAC	2 cut(s)	426, 484
Hpy188III	TCNNGA	8 cut(s)	292, 1063, 1265, 1414, 1448, 1667, 1705, 1732
Hpy8I	GTNNAC	2 cut(s)	426, 484
HpyCH4III	ACNGT	2 cut(s)	187, 926
HpyCH4IV	ACGT	2 cut(s)	108, 1442
HpyCH4V	TGCA	7 cut(s)	466, 493, 553, 915, 1007, 1353, 1582
HpySE526I	ACGT	2 cut(s)	108, 1442
Hsp92I	GRCGYC	3 cut(s)	162, 174, 264
Hsp92II	CATG	3 cut(s)	490, 1417, 1466
HspAI	GCGC	2 cut(s)	357, 594
KroI	GCCGGC	1 cut(s)	44
KroNI	GCCGGC	1 cut(s)	46
KspAI	GTTAAC	1 cut(s)	484
Kzo9I	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
LmnI	GCTCC	6 cut(s)	100, 236, 588, 682, 700, 1180
Lsp1109I	GCAGC	8 cut(s)	478, 481, 628, 769, 865, 1222, 1282, 1648
LweI	GCATC	4 cut(s)	480, 1090, 1105, 1330
MaeI	CTAG	6 cut(s)	513, 560, 993, 1139, 1169, 1527
MaeII	ACGT	2 cut(s)	108, 1442
MaeIII	GTNAC	1 cut(s)	926
MalI	GATC	7 cut(s)	127, 300, 1161, 1272, 1467, 1539, 1614
MboI	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
MfeI	CAATTG	1 cut(s)	1008
MflI	RGATCY	3 cut(s)	1270, 1537, 1612
MhlI	GDGCHC	3 cut(s)	195, 370, 376
MlsI	TGGCCA	1 cut(s)	1697
MluCI	AATT	6 cut(s)	1008, 1106, 1199, 1553, 1593, 1725
MluI	ACGCGT	1 cut(s)	402
MluNI	TGGCCA	1 cut(s)	1697
MlyI	GAGTC	1 cut(s)	346
MmeI	TCCRAC	5 cut(s)	144, 183, 195, 579, 609
Mox20I	TGGCCA	1 cut(s)	1697
Mph1103I	ATGCAT	1 cut(s)	917
MreI	CGCCGGCG	1 cut(s)	44
MroNI	GCCGGC	1 cut(s)	44
MroXI	GAANNNNTTC	2 cut(s)	392, 800
MscI	TGGCCA	1 cut(s)	1697
MseI	TTAA	7 cut(s)	318, 483, 758, 918, 969, 1518, 1737
Msp20I	TGGCCA	1 cut(s)	1697
MspA1I	CMGCKG	1 cut(s)	641
MspI	CCGG	9 cut(s)	45, 454, 473, 617, 674, 680, 686, 698, 716
MspR9I	CCNGG	7 cut(s)	454, 455, 587, 736, 1470, 1478, 1646
MunI	CAATTG	1 cut(s)	1008
Mva1269I	GAATGC	2 cut(s)	917, 1584
MvaI	CCWGG	5 cut(s)	587, 736, 1470, 1478, 1646
MvnI	CGCG	3 cut(s)	357, 404, 596
NaeI	GCCGGC	1 cut(s)	46
NciI	CCSGG	2 cut(s)	454, 455
NdeII	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
NgoMIV	GCCGGC	1 cut(s)	44
NheI	GCTAGC	2 cut(s)	1168, 1526
NlaIII	CATG	3 cut(s)	490, 1417, 1466
NlaIV	GGNNCC	8 cut(s)	232, 414, 678, 684, 696, 702, 1272, 1587
NmeAIII	GCCGAG	3 cut(s)	207, 228, 358
NmuCI	GTSAC	1 cut(s)	926
NsiI	ATGCAT	1 cut(s)	917
PaeR7I	CTCGAG	1 cut(s)	290
PagI	TCATGA	1 cut(s)	1413
PcsI	WCGNNNNNNNCGW	4 cut(s)	168, 255, 264, 443
PctI	GAATGC	2 cut(s)	917, 1584
PdiI	GCCGGC	1 cut(s)	46
PdmI	GAANNNNTTC	2 cut(s)	392, 800
PfeI	GAWTC	3 cut(s)	499, 871, 1247
PflMI	CCANNNNNTGG	1 cut(s)	1711
PfoI	TCCNGGA	1 cut(s)	1644
PleI	GAGTC	1 cut(s)	345
PpsI	GAGTC	1 cut(s)	345
PpuMI	RGGWCCY	3 cut(s)	413, 1135, 1585
PsiI	TTATAA	1 cut(s)	789
Psp124BI	GAGCTC	1 cut(s)	195
Psp5II	RGGWCCY	3 cut(s)	413, 1135, 1585
Psp6I	CCWGG	5 cut(s)	585, 734, 1468, 1476, 1644
PspGI	CCWGG	5 cut(s)	585, 734, 1468, 1476, 1644
PspN4I	GGNNCC	8 cut(s)	232, 414, 678, 684, 696, 702, 1272, 1587
PspPPI	RGGWCCY	3 cut(s)	413, 1135, 1585
PstNI	CAGNNNCTG	2 cut(s)	1616, 1711
PsuI	RGATCY	3 cut(s)	1270, 1537, 1612
RsaI	GTAC	2 cut(s)	451, 1220
RsaNI	GTAC	2 cut(s)	450, 1219
SacI	GAGCTC	1 cut(s)	195
SaqAI	TTAA	7 cut(s)	318, 483, 758, 918, 969, 1518, 1737
Sau3AI	GATC	7 cut(s)	125, 298, 1159, 1270, 1465, 1537, 1612
SchI	GAGTC	1 cut(s)	346
ScrFI	CCNGG	7 cut(s)	454, 455, 587, 736, 1470, 1478, 1646
SduI	GDGCHC	3 cut(s)	195, 370, 376
SfaNI	GCATC	4 cut(s)	480, 1090, 1105, 1330
SfcI	CTRYAG	1 cut(s)	217
Sfr274I	CTCGAG	1 cut(s)	290
SgrAI	CRCCGGYG	1 cut(s)	44
SinI	GGWCC	6 cut(s)	413, 426, 538, 1135, 1402, 1585
SlaI	CTCGAG	1 cut(s)	290
SmaI	CCCGGG	1 cut(s)	455
SmlI	CTYRAG	2 cut(s)	194, 290
SmoI	CTYRAG	2 cut(s)	194, 290
Sse9I	AATT	6 cut(s)	1008, 1106, 1199, 1553, 1593, 1725
SspMI	CTAG	6 cut(s)	513, 560, 993, 1139, 1169, 1527
SstI	GAGCTC	1 cut(s)	195
StyD4I	CCNGG	7 cut(s)	452, 453, 585, 734, 1468, 1476, 1644
StyI	CCWWGG	3 cut(s)	21, 559, 828
TaaI	ACNGT	2 cut(s)	187, 926
TaiI	ACGT	2 cut(s)	111, 1445
TaqI	TCGA	5 cut(s)	189, 258, 291, 804, 1031
TasI	AATT	6 cut(s)	1008, 1106, 1199, 1553, 1593, 1725
TauI	GCSGC	9 cut(s)	35, 62, 225, 314, 353, 362, 382, 641, 652
TfiI	GAWTC	3 cut(s)	499, 871, 1247
Tru1I	TTAA	7 cut(s)	318, 483, 758, 918, 969, 1518, 1737
Tru9I	TTAA	7 cut(s)	318, 483, 758, 918, 969, 1518, 1737
TscAI	CASTG	3 cut(s)	814, 1249, 1723
TseFI	GTSAC	1 cut(s)	926
TseI	GCWGC	8 cut(s)	466, 469, 641, 782, 878, 1210, 1295, 1661
Tsp45I	GTSAC	1 cut(s)	926
TspDTI	ATGAA	5 cut(s)	864, 924, 1402, 1440, 1566
TspGWI	ACGGA	1 cut(s)	615
TspMI	CCCGGG	1 cut(s)	453
TspRI	CASTG	3 cut(s)	814, 1249, 1723
Van91I	CCANNNNNTGG	1 cut(s)	1711
VpaK11BI	GGWCC	6 cut(s)	413, 426, 538, 1135, 1402, 1585
XagI	CCTNNNNNAGG	1 cut(s)	320
XapI	RAATTY	2 cut(s)	1199, 1593
XhoI	CTCGAG	1 cut(s)	290
XmaI	CCCGGG	1 cut(s)	453
XmaJI	CCTAGG	1 cut(s)	559
XmnI	GAANNNNTTC	2 cut(s)	392, 800
XspI	CTAG	6 cut(s)	513, 560, 993, 1139, 1169, 1527
Zsp2I	ATGCAT	1 cut(s)	917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.