pycom01g09340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005165
Physical Location & Seq
Forward (+)
10290848 .. 10291787
940 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09340.1

Sequence Viewer

Length: 570 bp
ATGTCGTTCATAAAAGGAGATTTACTTACGAGAACCAGAAAGCTGGTGAAGGGCCTGGCCAAGGCCGAGCCTGTTTGGCTCAAAGCCATGGAAAAGGCACCGCCTGTAACGTTTCCTCGTGCAAATGCAATTCAGAAAATCACTCTCCCAGAGGATGTTTACATAAAGAAGTTTTTCCAGAAGCATCCAGATTCCAAACATGAAGATGCCATCAAGATTTCTTCTTTTGATCCTCCTCCAGCCCGTATATTTGGTTTGCGGGTACTTGAGTTGAAAGAGCAAGGTGTTGCTGAGGAAGAAGCAATGGCAGTAGCTGATATGGAATACCGGATGGAGAGAAAAGAGAAGAAGAAGGCATATTCCCGCTTGAAGAAAATTGCACGCCTTCAAGGGAAGAGGCCTCCTCCAAACCCATATCCCAGCGCTATCAAGGAAATACAAGCTGAGGAGAAGAAGTATGTTCGCGACCGTTTTTACAATCCCAAAATTATTCAACTTGTAAGGCAATTGCAAGAGGACAAGGCAGCTGAAGCGCAGGAGAGATTCAGAGCAGGAGGAGGTTCCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

21.83

Weight (kDa)

9.85

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 1 cut(s) 465
AciI CCGC 3 cut(s) 101, 259, 364
AclI AACGTT 1 cut(s) 110
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 1 cut(s) 264
AfeI AGCGCT 1 cut(s) 424
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 4 cut(s) 274, 370, 389, 494
AjnI CCWGG 2 cut(s) 54, 563
AluBI AGCT 4 cut(s) 43, 314, 443, 527
AluI AGCT 4 cut(s) 43, 314, 443, 527
AlwI GGATC 1 cut(s) 224
AlwNI CAGNNNCTG 1 cut(s) 314
Aor51HI AGCGCT 1 cut(s) 424
AoxI GGCC 4 cut(s) 52, 57, 63, 398
ApeKI GCWGC 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 2 cut(s) 425, 535
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 58
BaeI ACNNNNGTAYC 2 cut(s) 254, 287
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 1 cut(s) 97
BauI CACGAG 1 cut(s) 117
BbvCI CCTCAGC 2 cut(s) 291, 444
BbvI GCAGC 1 cut(s) 536
BccI CCATC 2 cut(s) 218, 325
BciT130I CCWGG 2 cut(s) 56, 565
BfoI RGCGCY 1 cut(s) 426
BglI GCCNNNNNGGC 1 cut(s) 76
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
Bme1390I CCNGG 2 cut(s) 56, 565
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 99, 562
BmrFI CCNGG 2 cut(s) 56, 565
BmsI GCATC 2 cut(s) 193, 196
BplI GAGNNNNNCTC 2 cut(s) 388, 420
BpmI CTGGAG 1 cut(s) 222
Bpu10I CCTNAGC 2 cut(s) 291, 444
BpuEI CTTGAG 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 60, 87
BsaWI WCCGGW 1 cut(s) 327
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 2 cut(s) 56, 565
BseDI CCNNGG 2 cut(s) 60, 87
BseGI GGATG 3 cut(s) 160, 184, 336
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 2 cut(s) 282, 435
BseRI GAGGAG 4 cut(s) 225, 393, 461, 570
BseXI GCAGC 1 cut(s) 536
BseYI CCCAGC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 465
Bsh1285I CGRYCG 1 cut(s) 469
BshFI GGCC 4 cut(s) 54, 59, 65, 400
BshNI GGYRCC 1 cut(s) 97
BsiEI CGRYCG 1 cut(s) 469
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 61
BsnI GGCC 4 cut(s) 54, 59, 65, 400
Bsp143I GATC 1 cut(s) 229
Bsp19I CCATGG 1 cut(s) 87
Bsp68I TCGCGA 1 cut(s) 465
BspACI CCGC 3 cut(s) 101, 259, 364
BspANI GGCC 4 cut(s) 54, 59, 65, 400
BspCNI CTCAG 2 cut(s) 283, 436
BspFNI CGCG 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 99, 562
BspPI GGATC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 60, 87
BssMI GATC 1 cut(s) 229
BssSI CACGAG 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 60, 87
Bst2BI CACGAG 1 cut(s) 117
Bst2UI CCWGG 2 cut(s) 56, 565
Bst4CI ACNGT 1 cut(s) 470
Bst6I CTCTTC 1 cut(s) 389
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 2 cut(s) 291, 444
BstDSI CCRYGG 1 cut(s) 87
BstENI CCTNNNNNAGG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 160, 184, 336
BstFNI CGCG 1 cut(s) 465
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 425, 535
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstMCI CGRYCG 1 cut(s) 469
BstMWI GCNNNNNNNGC 2 cut(s) 76, 530
BstNI CCWGG 2 cut(s) 56, 565
BstSCI CCNGG 2 cut(s) 54, 563
BstUI CGCG 1 cut(s) 465
BstV1I GCAGC 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 43
BsuRI GGCC 4 cut(s) 54, 59, 65, 400
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 3 cut(s) 160, 184, 336
BtuMI TCGCGA 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 382
CaiI CAGNNNCTG 1 cut(s) 314
CfoI GCGC 2 cut(s) 425, 535
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 263
CviAII CATG 2 cut(s) 88, 200
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 2 cut(s) 291, 444
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 60, 87
Eco147I AGGCCT 1 cut(s) 400
Eco47III AGCGCT 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 549
EcoNI CCTNNNNNAGG 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 2 cut(s) 54, 563
EcoT14I CCWWGG 2 cut(s) 60, 87
ErhI CCWWGG 2 cut(s) 60, 87
FaeI CATG 2 cut(s) 91, 203
FaiI YATR 9 cut(s) 11, 89, 164, 201, 248, 320, 358, 415, 459
FatI CATG 2 cut(s) 87, 199
FauI CCCGC 2 cut(s) 252, 371
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 3 cut(s) 167, 171, 343
Fsp4HI GCNGC 1 cut(s) 525
GlaI GCGC 2 cut(s) 424, 534
GluI GCNGC 1 cut(s) 525
GsaI CCCAGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 4 cut(s) 54, 59, 65, 400
HapII CCGG 1 cut(s) 328
HhaI GCGC 2 cut(s) 425, 535
Hin1II CATG 2 cut(s) 91, 203
Hin6I GCGC 2 cut(s) 423, 533
HinP1I GCGC 2 cut(s) 423, 533
HinfI GANTC 2 cut(s) 191, 543
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 135, 548
Hpy188III TCNNGA 4 cut(s) 178, 188, 214, 464
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 3 cut(s) 43, 346, 395
HpyCH4III ACNGT 1 cut(s) 470
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 4 cut(s) 122, 128, 380, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 530
HpyF3I CTNAG 2 cut(s) 291, 444
HpySE526I ACGT 1 cut(s) 110
Hsp92II CATG 2 cut(s) 91, 203
HspAI GCGC 2 cut(s) 423, 533
Kzo9I GATC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 536
LweI GCATC 2 cut(s) 193, 196
MaeII ACGT 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 8 cut(s) 213, 215, 308, 358, 361, 382, 406, 463
MfeI CAATTG 1 cut(s) 506
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 4 cut(s) 129, 375, 486, 506
MluNI TGGCCA 1 cut(s) 59
Mox20I TGGCCA 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 59
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 2 cut(s) 56, 565
MunI CAATTG 1 cut(s) 506
MvaI CCWGG 2 cut(s) 56, 565
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 2 cut(s) 76, 530
NcoI CCATGG 1 cut(s) 87
NdeII GATC 1 cut(s) 229
NlaIII CATG 2 cut(s) 91, 203
NlaIV GGNNCC 2 cut(s) 99, 562
NmeAIII GCCGAG 1 cut(s) 91
NruI TCGCGA 1 cut(s) 465
PceI AGGCCT 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 191, 543
PkrI GCNGC 1 cut(s) 526
Psp1406I AACGTT 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 54, 563
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 2 cut(s) 54, 563
PspN4I GGNNCC 2 cut(s) 99, 562
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 527
RruI TCGCGA 1 cut(s) 465
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
RseI CAYNNNNRTG 1 cut(s) 204
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 1 cut(s) 52
ScrFI CCNGG 2 cut(s) 56, 565
SetI ASST 7 cut(s) 45, 113, 286, 316, 445, 529, 562
SfaNI GCATC 2 cut(s) 193, 196
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 4 cut(s) 129, 375, 486, 506
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 3 cut(s) 101, 259, 364
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 2 cut(s) 54, 563
StyI CCWWGG 2 cut(s) 60, 87
TaaI ACNGT 1 cut(s) 470
TaiI ACGT 1 cut(s) 113
TasI AATT 4 cut(s) 129, 375, 486, 506
TfiI GAWTC 2 cut(s) 191, 543
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 1 cut(s) 216
XagI CCTNNNNNAGG 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.