MD07G1136300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005165
Physical Location & Seq
Reverse (-)
19844070 .. 19845768
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1136300.v1.1.491

Sequence Viewer

Length: 570 bp
ATGTCGTTCATGAAAGGAGATTTACTTACGAGAACTAGAAAGCTGGTGAAGGGCCTAGCCAAGGCCGAGCCTGTTTGGCTCAAAGCCATGGAAAAGGCACCGCCTGTAACATTTCCTCGTGCAAATGCAATTCAGAAAATCACTCTCCCAGAGGATGTTTACATAAAGAAGTTTTTCCAGAAGCATCCAGATTCCAAACATGAAGATGCCATAAAGATTTCTTCTTTTGATCCTCCACCAGCCCGTATATTTGGTTTGCGGGTGCTTGAGTTGAAAGAGCAAGGGGTTTCTGAGGAAGAAGCAATGGCAGTAGCCGATATGGAATACCGGATGGAGAGAAAAGAGAAGAAGAAGGCATATTCCCGCTTGAAGAAAATTGCACACCTTCAAGGGAAGAGGCCTCCTCCCAACCCATATCCCAGCGCTATCAAGGAAATACAAGCTGAGGAGAAGAAGTATGTTCGCGACCGCTTTTACAATCCCAAAATTATTCAACTTGTAAGGAAATTGCAAGAGGAGAAGGTAGCTGAAGCGCAGGAGAGATTCAGAGGAGGAGGAGGTTCCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

21.88

Weight (kDa)

9.83

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 1 cut(s) 465
AciI CCGC 4 cut(s) 101, 259, 364, 469
AclWI GGATC 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 549
AfeI AGCGCT 1 cut(s) 424
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 4 cut(s) 274, 370, 389, 494
AjnI CCWGG 1 cut(s) 563
AluBI AGCT 3 cut(s) 43, 443, 527
AluI AGCT 3 cut(s) 43, 443, 527
AlwI GGATC 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 424
AoxI GGCC 3 cut(s) 52, 63, 398
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 2 cut(s) 425, 535
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 58
BanI GGYRCC 1 cut(s) 97
BauI CACGAG 1 cut(s) 117
BbvCI CCTCAGC 1 cut(s) 444
BccI CCATC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 565
BfaI CTAG 2 cut(s) 36, 56
BfoI RGCGCY 1 cut(s) 426
BglI GCCNNNNNGGC 1 cut(s) 76
Bme1390I CCNGG 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 99, 562
BmrFI CCNGG 1 cut(s) 565
BmsI GCATC 2 cut(s) 193, 196
BplI GAGNNNNNCTC 2 cut(s) 388, 420
Bpu10I CCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 60, 87
BsaWI WCCGGW 1 cut(s) 327
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 565
BseDI CCNNGG 2 cut(s) 60, 87
BseGI GGATG 3 cut(s) 160, 184, 336
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 2 cut(s) 282, 435
BseRI GAGGAG 6 cut(s) 393, 461, 530, 564, 567, 570
BseYI CCCAGC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 465
Bsh1285I CGRYCG 1 cut(s) 469
BshFI GGCC 3 cut(s) 54, 65, 400
BshNI GGYRCC 1 cut(s) 97
BsiEI CGRYCG 1 cut(s) 469
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 61
BsnI GGCC 3 cut(s) 54, 65, 400
Bsp143I GATC 1 cut(s) 229
Bsp19I CCATGG 1 cut(s) 87
Bsp68I TCGCGA 1 cut(s) 465
BspACI CCGC 4 cut(s) 101, 259, 364, 469
BspANI GGCC 3 cut(s) 54, 65, 400
BspCNI CTCAG 2 cut(s) 283, 436
BspFNI CGCG 1 cut(s) 465
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 2 cut(s) 99, 562
BspPI GGATC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 60, 87
BssMI GATC 1 cut(s) 229
BssSI CACGAG 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 60, 87
Bst2BI CACGAG 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 565
Bst6I CTCTTC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 291, 444
BstDSI CCRYGG 1 cut(s) 87
BstENI CCTNNNNNAGG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 160, 184, 336
BstFNI CGCG 1 cut(s) 465
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 425, 535
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstMCI CGRYCG 1 cut(s) 469
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstNI CCWGG 1 cut(s) 565
BstSCI CCNGG 1 cut(s) 563
BstUI CGCG 1 cut(s) 465
BsuRI GGCC 3 cut(s) 54, 65, 400
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 3 cut(s) 160, 184, 336
BtuMI TCGCGA 1 cut(s) 465
CciI TCATGA 1 cut(s) 9
CfoI GCGC 2 cut(s) 425, 535
Cfr13I GGNCC 1 cut(s) 52
CviAII CATG 3 cut(s) 10, 88, 200
DdeI CTNAG 2 cut(s) 291, 444
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 60, 87
Eco147I AGGCCT 1 cut(s) 400
Eco47III AGCGCT 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 549
EcoNI CCTNNNNNAGG 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 563
EcoT14I CCWWGG 2 cut(s) 60, 87
ErhI CCWWGG 2 cut(s) 60, 87
FaeI CATG 3 cut(s) 13, 91, 203
FatI CATG 3 cut(s) 9, 87, 199
FauI CCCGC 2 cut(s) 252, 371
FokI GGATG 3 cut(s) 167, 171, 343
FspBI CTAG 2 cut(s) 36, 56
GlaI GCGC 2 cut(s) 424, 534
GsaI CCCAGC 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 3 cut(s) 54, 65, 400
HapII CCGG 1 cut(s) 328
HhaI GCGC 2 cut(s) 425, 535
Hin1II CATG 3 cut(s) 13, 91, 203
Hin6I GCGC 2 cut(s) 423, 533
HinP1I GCGC 2 cut(s) 423, 533
HinfI GANTC 2 cut(s) 191, 543
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 3 cut(s) 135, 292, 548
Hpy188III TCNNGA 4 cut(s) 10, 178, 188, 464
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 4 cut(s) 43, 346, 395, 514
HpyCH4V TGCA 4 cut(s) 122, 128, 380, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpyF3I CTNAG 2 cut(s) 291, 444
Hsp92II CATG 3 cut(s) 13, 91, 203
HspAI GCGC 2 cut(s) 423, 533
Kzo9I GATC 1 cut(s) 229
LweI GCATC 2 cut(s) 193, 196
MaeI CTAG 2 cut(s) 36, 56
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 8 cut(s) 213, 215, 308, 358, 361, 382, 406, 463
MluCI AATT 4 cut(s) 129, 375, 486, 506
MroXI GAANNNNTTC 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 204
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 565
MvaI CCWGG 1 cut(s) 565
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 76
NcoI CCATGG 1 cut(s) 87
NdeII GATC 1 cut(s) 229
NlaIII CATG 3 cut(s) 13, 91, 203
NlaIV GGNNCC 2 cut(s) 99, 562
NmeAIII GCCGAG 1 cut(s) 91
NruI TCGCGA 1 cut(s) 465
PagI TCATGA 1 cut(s) 9
PceI AGGCCT 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 191, 543
Psp6I CCWGG 1 cut(s) 563
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 99, 562
PspPI GGNCC 1 cut(s) 52
RruI TCGCGA 1 cut(s) 465
RseI CAYNNNNRTG 1 cut(s) 204
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 565
SetI ASST 6 cut(s) 45, 387, 445, 525, 529, 562
SfaNI GCATC 2 cut(s) 193, 196
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 4 cut(s) 129, 375, 486, 506
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 4 cut(s) 101, 259, 364, 469
SspMI CTAG 2 cut(s) 36, 56
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 563
StyI CCWWGG 2 cut(s) 60, 87
TasI AATT 4 cut(s) 129, 375, 486, 506
TfiI GAWTC 2 cut(s) 191, 543
TspDTI ATGAA 2 cut(s) 26, 216
XagI CCTNNNNNAGG 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 2 cut(s) 36, 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.