Rh7BG325400

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013922
Physical Location & Seq
Forward (+)
32114803 .. 32116397
1595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG325400.1

Sequence Viewer

Length: 822 bp
ATGAGTCGATCACCAAAAACCTATCCAAGTTTCAACTATAAGCTTCTTCTCCTCGGAGTTTTCCTGGCTTTCGTTGTCCTCTTTGTCTTGAGATCAAACTTCTCATCATCTGATTCCAATCAAAATCCATCTACCATCTCGCCAAAGGTACCTTTACTCAATGCTTCGGCTAAAGATTTAAGTAGCACTTCTACAGACTGCTCACCAAGTACTACTGGTTGCAACAAGATCCCATCCTCTCTAGCTCAAACCATAATCCACTACACAACCTCAACCATCACCCCACAACAAACCCTCAAGGAAATCTCAGTGACAGCAAAGATTCTAGACCAGAAATCACCATGCAACTTCCTTGTCTTTGGCCTTGGCCACGACAGCCTTATGTGGAGTGCACTCAACCATGGGGGTAGGACAATTTTCCTGGAAGAAGACGAGTCCTGGATCGAGCAAATCCGACGCCGCTTCCCCACATTGGAATCATACCATGTCACATATAACAGCAAGGTGAATGAGGCTGATAATCTCATGGATGTTGGGAAGGGGCCTGAGTGCACTGCAGTTGGTAGTGATATTAAGTACAGCATGTGCCAATTAGCTTTGAAAGGTCTACCGAGTGAGGTTTATGACATCAAATGGGATTTGATAATGGTGGATGCACCCACCGGGTATCATGATGAAGCACCGGGAAGAATGAGTGCCATATACACAGCTGGAATGATAGCCCGAAATAAAGAGGAAGGGGAGACTACTCATGTGTTTGTTCATGATGTGAACAGAGTTGTGGAAGACAAGTTCTCTATGTCATTCCTTTGTAAAGGTTGA

Protein Analysis

273

Amino Acids

30.24

Weight (kDa)

5.98

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 100 - 272 3.2e-75 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013922)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09610
fragaria_vesca FvH4_5g28110
malus_domestica MD08G1244700.v1.1 MD15G1439800.v1.1
prunus_persica Prupe.1G582100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0271041
rosa_laevigata RLG00000002227
rosa_multiflora Rmu_co8384627.1_g000001
rosa_roxburghii Rroxscaffold_7G00197370
rosa_rugosa Rorug06G0058100 Rorug07G0193100
rosa_samantha Rh6AG177900 Rh6BG181200 Rh6CG177000 Rh6DG169200 Rh7BG325400
rosa_wichuraiana Rw6G015180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 607
AciI CCGC 1 cut(s) 460
AclWI GGATC 2 cut(s) 223, 449
AcoI YGGCCR 1 cut(s) 367
AcyI GRCGYC 1 cut(s) 457
AfaI GTAC 3 cut(s) 150, 211, 578
AfiI CCNNNNNNNGG 1 cut(s) 472
AgsI TTSAA 2 cut(s) 34, 601
AjnI CCWGG 3 cut(s) 63, 420, 437
AloI GAACNNNNNNTCC 2 cut(s) 776, 808
AluBI AGCT 4 cut(s) 43, 245, 596, 710
AluI AGCT 4 cut(s) 43, 245, 596, 710
Alw21I GWGCWC 2 cut(s) 394, 554
Alw26I GTCTC 1 cut(s) 737
Alw44I GTGCAC 2 cut(s) 390, 550
AlwI GGATC 2 cut(s) 223, 449
AoxI GGCC 3 cut(s) 361, 367, 542
ApaLI GTGCAC 2 cut(s) 390, 550
Asp718I GGTACC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 542
AsuC2I CCSGG 2 cut(s) 664, 684
AsuHPI GGTGA 5 cut(s) 3, 195, 271, 330, 517
BaeGI GKGCMC 2 cut(s) 394, 554
BalI TGGCCA 1 cut(s) 369
BanI GGYRCC 1 cut(s) 148
BbsI GAAGAC 2 cut(s) 435, 792
Bbv12I GWGCWC 2 cut(s) 394, 554
BccI CCATC 4 cut(s) 136, 143, 241, 284
BciT130I CCWGG 3 cut(s) 65, 422, 439
BcnI CCSGG 2 cut(s) 664, 684
BcoDI GTCTC 1 cut(s) 737
BfaI CTAG 2 cut(s) 242, 326
BfmI CTRYAG 2 cut(s) 192, 555
BisI GCNGC 1 cut(s) 460
BlsI GCNGC 1 cut(s) 461
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 5 cut(s) 65, 422, 439, 664, 684
BmgT120I GGNCC 1 cut(s) 542
BmiI GGNNCC 2 cut(s) 150, 543
BmrFI CCNGG 5 cut(s) 65, 422, 439, 664, 684
BmsI GCATC 1 cut(s) 643
BpiI GAAGAC 2 cut(s) 435, 792
BpuEI CTTGAG 2 cut(s) 109, 281
BpuMI CCSGG 2 cut(s) 664, 684
BsaBI GATNNNNATC 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 457
BsaJI CCNNGG 3 cut(s) 52, 364, 400
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse1I ACTGG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 3 cut(s) 65, 422, 439
BseDI CCNNGG 3 cut(s) 52, 364, 400
BseGI GGATG 3 cut(s) 233, 535, 658
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 472
BseMII CTCAG 2 cut(s) 321, 537
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 41
BseSI GKGCMC 2 cut(s) 394, 554
BshFI GGCC 3 cut(s) 363, 369, 544
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 394, 554
BsiSI CCGG 2 cut(s) 663, 683
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 1 cut(s) 737
BsnI GGCC 3 cut(s) 363, 369, 544
Bsp1286I GDGCHC 2 cut(s) 394, 554
Bsp143I GATC 4 cut(s) 8, 92, 228, 441
Bsp19I CCATGG 1 cut(s) 400
BspACI CCGC 1 cut(s) 460
BspANI GGCC 3 cut(s) 363, 369, 544
BspCNI CTCAG 2 cut(s) 320, 538
BspHI TCATGA 2 cut(s) 670, 763
BspLI GGNNCC 2 cut(s) 150, 543
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 2 cut(s) 223, 449
BspT107I GGYRCC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 3 cut(s) 52, 364, 400
BssMI GATC 4 cut(s) 8, 92, 228, 441
BssNI GRCGYC 1 cut(s) 457
BssT1I CCWWGG 2 cut(s) 364, 400
Bst2UI CCWGG 3 cut(s) 65, 422, 439
BstACI GRCGYC 1 cut(s) 457
BstDEI CTNAG 2 cut(s) 307, 546
BstDSI CCRYGG 1 cut(s) 400
BstF5I GGATG 3 cut(s) 233, 535, 658
BstKTI GATC 4 cut(s) 11, 95, 231, 444
BstMAI GTCTC 1 cut(s) 737
BstMBI GATC 4 cut(s) 8, 92, 228, 441
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNI CCWGG 3 cut(s) 65, 422, 439
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 5 cut(s) 63, 420, 437, 662, 682
BstSFI CTRYAG 2 cut(s) 192, 555
BstSLI GKGCMC 2 cut(s) 394, 554
BstV2I GAAGAC 2 cut(s) 435, 792
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
BsuRI GGCC 3 cut(s) 363, 369, 544
BtgI CCRYGG 1 cut(s) 400
BtsCI GGATG 3 cut(s) 233, 535, 658
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 315, 552
CciI TCATGA 2 cut(s) 670, 763
Cfr13I GGNCC 1 cut(s) 542
CseI GACGC 1 cut(s) 465
Csp6I GTAC 3 cut(s) 149, 210, 577
CviAII CATG 8 cut(s) 342, 401, 485, 526, 583, 671, 752, 764
CviQI GTAC 3 cut(s) 149, 210, 577
DdeI CTNAG 2 cut(s) 307, 546
DpnI GATC 4 cut(s) 10, 94, 230, 443
DpnII GATC 4 cut(s) 8, 92, 228, 441
EaeI YGGCCR 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 364, 400
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 3 cut(s) 63, 420, 437
EcoT14I CCWWGG 2 cut(s) 364, 400
ErhI CCWWGG 2 cut(s) 364, 400
FaeI CATG 8 cut(s) 345, 404, 488, 529, 586, 674, 755, 767
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FatI CATG 8 cut(s) 341, 400, 484, 525, 582, 670, 751, 763
FblI GTMKAC 1 cut(s) 607
Fnu4HI GCNGC 1 cut(s) 460
FokI GGATG 3 cut(s) 220, 542, 665
Fsp4HI GCNGC 1 cut(s) 460
FspBI CTAG 2 cut(s) 242, 326
GluI GCNGC 1 cut(s) 460
HaeIII GGCC 3 cut(s) 363, 369, 544
HapII CCGG 2 cut(s) 663, 683
HgaI GACGC 1 cut(s) 465
Hin1I GRCGYC 1 cut(s) 457
Hin1II CATG 8 cut(s) 345, 404, 488, 529, 586, 674, 755, 767
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 5 cut(s) 4, 113, 322, 434, 476
HpaII CCGG 2 cut(s) 663, 683
HphI GGTGA 5 cut(s) 3, 195, 271, 330, 517
Hpy166II GTNNAC 4 cut(s) 392, 552, 608, 772
Hpy188I TCNGA 3 cut(s) 56, 112, 455
Hpy188III TCNNGA 4 cut(s) 88, 326, 671, 764
Hpy8I GTNNAC 4 cut(s) 392, 552, 608, 772
Hpy99I CGWCG 1 cut(s) 459
HpyAV CCTTC 2 cut(s) 532, 731
HpyCH4V TGCA 6 cut(s) 222, 345, 392, 552, 557, 656
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpyF3I CTNAG 2 cut(s) 307, 546
Hsp92I GRCGYC 1 cut(s) 457
Hsp92II CATG 8 cut(s) 345, 404, 488, 529, 586, 674, 755, 767
KpnI GGTACC 1 cut(s) 152
Kzo9I GATC 4 cut(s) 8, 92, 228, 441
LweI GCATC 1 cut(s) 643
MaeI CTAG 2 cut(s) 242, 326
MaeIII GTNAC 2 cut(s) 310, 487
MalI GATC 4 cut(s) 10, 94, 230, 443
MboI GATC 4 cut(s) 8, 92, 228, 441
MboII GAAGA 5 cut(s) 38, 437, 440, 699, 797
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 394, 554
MlsI TGGCCA 1 cut(s) 369
MluCI AATT 2 cut(s) 414, 590
MluNI TGGCCA 1 cut(s) 369
MlyI GAGTC 2 cut(s) 13, 443
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 8 cut(s) 62, 89, 247, 280, 305, 505, 610, 727
Mox20I TGGCCA 1 cut(s) 369
MscI TGGCCA 1 cut(s) 369
MseI TTAA 2 cut(s) 179, 573
Msp20I TGGCCA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 2 cut(s) 663, 683
MspR9I CCNGG 5 cut(s) 65, 422, 439, 664, 684
MvaI CCWGG 3 cut(s) 65, 422, 439
MwoI GCNNNNNNNGC 1 cut(s) 375
NciI CCSGG 2 cut(s) 664, 684
NcoI CCATGG 1 cut(s) 400
NdeII GATC 4 cut(s) 8, 92, 228, 441
NlaIII CATG 8 cut(s) 345, 404, 488, 529, 586, 674, 755, 767
NlaIV GGNNCC 2 cut(s) 150, 543
NmuCI GTSAC 2 cut(s) 310, 487
NspI RCATGY 1 cut(s) 586
PagI TCATGA 2 cut(s) 670, 763
PfeI GAWTC 3 cut(s) 113, 322, 476
PfoI TCCNGGA 2 cut(s) 420, 437
PkrI GCNGC 1 cut(s) 461
PleI GAGTC 2 cut(s) 12, 442
PpsI GAGTC 2 cut(s) 12, 442
Psp6I CCWGG 3 cut(s) 63, 420, 437
PspGI CCWGG 3 cut(s) 63, 420, 437
PspN4I GGNNCC 2 cut(s) 150, 543
PspPI GGNCC 1 cut(s) 542
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 228
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 3 cut(s) 150, 211, 578
RsaNI GTAC 3 cut(s) 149, 210, 577
SaqAI TTAA 2 cut(s) 179, 573
SatI GCNGC 1 cut(s) 460
Sau3AI GATC 4 cut(s) 8, 92, 228, 441
Sau96I GGNCC 1 cut(s) 542
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 2 cut(s) 13, 443
ScrFI CCNGG 5 cut(s) 65, 422, 439, 664, 684
SduI GDGCHC 2 cut(s) 394, 554
SfaNI GCATC 1 cut(s) 643
SfcI CTRYAG 2 cut(s) 192, 555
SmlI CTYRAG 2 cut(s) 88, 296
SmoI CTYRAG 2 cut(s) 88, 296
Sse9I AATT 2 cut(s) 414, 590
SsiI CCGC 1 cut(s) 460
SspMI CTAG 2 cut(s) 242, 326
StyD4I CCNGG 5 cut(s) 63, 420, 437, 662, 682
StyI CCWWGG 2 cut(s) 364, 400
TaqI TCGA 2 cut(s) 7, 444
TasI AATT 2 cut(s) 414, 590
TatI WGTACW 2 cut(s) 209, 576
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 3 cut(s) 113, 322, 476
Tru1I TTAA 2 cut(s) 179, 573
Tru9I TTAA 2 cut(s) 179, 573
TscAI CASTG 2 cut(s) 315, 559
TseFI GTSAC 2 cut(s) 310, 487
Tsp45I GTSAC 2 cut(s) 310, 487
TspDTI ATGAA 2 cut(s) 690, 752
TspRI CASTG 2 cut(s) 315, 559
VneI GTGCAC 2 cut(s) 390, 550
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 1 cut(s) 586
XmiI GTMKAC 1 cut(s) 607
XspI CTAG 2 cut(s) 242, 326
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.