RchiOBHm_Chr6g0271041

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013922
Physical Location & Seq
Reverse (-)
29082002 .. 29083221
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24314

Sequence Viewer

Length: 909 bp
ATGAGTCGATCACCAAAAACCTATCCAAGTTTCAACTATAAGCTTCTTCTCCTCGGAGTTTTCCTGGCTTTCGTTGTCCTCTTTGTCTTGAGATCAAGCTTCTCATCATCTGATTCCAATCAAAATTCATCTACCATCTCACCAAAGGTACCTTTCCTCAATCCTTCGGCTAAAGATTTAAGTAGCACTTCTACAGACTGCTCACCAAGTACTAATATTCCTTGCAACAAGATCCCATCCTCTCTAGTTCAAACCATAATCCACTACACAACCTCAACCATCACCCCACAACAAACCCTCAAGGAAATCTCAGTGACAGCAAAGATTCTAGACCAGAAATCACCATGCAACTTCCTTGTCTTTGGCCTTGGCCACGACAGCCTTATGTGGAGTGCACTCAACCATGGGGGTAGGACAATTTTCCTGGAAGAAGACGAGTCCTGGATCGAGCAAATCCGACGCCGCTTCCCCACATTGGAATCATACCATGTCACATATAACAGCAAGGTGAATGAGGCTGATAATCTCATGGATGTTGGGAAGGGGCCTGAGTGCACTGCAGTTGGTAGTGATATTAAGTACAGCATGTGCCAATTGGCTTTGAAGGGTCTACCGAGTGAAGTTTATGACATCAAATGGGATTTGATAATGGTGGATGCACCCACCGGGTATCATGATGAAGCACCGGGAAGAATGAGTGCCATATACACAGCTGGAATGATAGCCCGAAATAAAGAGGAAGGGGAGACTACTCATGTGTTTGTTCATGATGTGAACAGAGTTGTGGAAGACAAGTTCTCTATGTCATTCCTTTGTAAAGGTTACATGCAAAAACAGGAAGGGAGGTTGAGGCACTTCACTATTCCTAGTCACAGGGATGGTTCTAGTAGGCCATTTTGCCCTGAATGA

Protein Analysis

302

Amino Acids

33.8

Weight (kDa)

6.51

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 101 - 286 4.3e-80 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013922)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09610
fragaria_vesca FvH4_5g28110
malus_domestica MD08G1244700.v1.1 MD15G1439800.v1.1
prunus_persica Prupe.1G582100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0271041
rosa_laevigata RLG00000002227
rosa_multiflora Rmu_co8384627.1_g000001
rosa_roxburghii Rroxscaffold_7G00197370
rosa_rugosa Rorug06G0058100 Rorug07G0193100
rosa_samantha Rh6AG177900 Rh6BG181200 Rh6CG177000 Rh6DG169200 Rh7BG325400
rosa_wichuraiana Rw6G015180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 610
AciI CCGC 1 cut(s) 463
AclWI GGATC 2 cut(s) 226, 452
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 460
AfaI GTAC 3 cut(s) 150, 211, 581
AfiI CCNNNNNNNGG 1 cut(s) 475
AgsI TTSAA 3 cut(s) 34, 251, 604
AjnI CCWGG 3 cut(s) 63, 423, 440
AloI GAACNNNNNNTCC 2 cut(s) 779, 811
AluBI AGCT 3 cut(s) 43, 99, 713
AluI AGCT 3 cut(s) 43, 99, 713
Alw21I GWGCWC 2 cut(s) 397, 557
Alw26I GTCTC 1 cut(s) 740
Alw44I GTGCAC 2 cut(s) 393, 553
AlwI GGATC 2 cut(s) 226, 452
AoxI GGCC 4 cut(s) 364, 370, 545, 890
ApaLI GTGCAC 2 cut(s) 393, 553
ApoI RAATTY 1 cut(s) 124
Asp718I GGTACC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 545
AsuC2I CCSGG 2 cut(s) 667, 687
AsuHPI GGTGA 6 cut(s) 3, 132, 195, 274, 333, 520
BaeGI GKGCMC 2 cut(s) 397, 557
BalI TGGCCA 1 cut(s) 372
BanI GGYRCC 1 cut(s) 148
BbsI GAAGAC 2 cut(s) 438, 795
Bbv12I GWGCWC 2 cut(s) 397, 557
BccI CCATC 4 cut(s) 143, 244, 287, 872
BciT130I CCWGG 3 cut(s) 65, 425, 442
BcnI CCSGG 2 cut(s) 667, 687
BcoDI GTCTC 1 cut(s) 740
BfaI CTAG 4 cut(s) 245, 329, 867, 885
BfmI CTRYAG 2 cut(s) 192, 558
BisI GCNGC 1 cut(s) 463
BlsI GCNGC 1 cut(s) 464
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 5 cut(s) 65, 425, 442, 667, 687
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 2 cut(s) 150, 546
BmrFI CCNGG 5 cut(s) 65, 425, 442, 667, 687
BmsI GCATC 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 438, 795
BpuEI CTTGAG 2 cut(s) 109, 284
BpuMI CCSGG 2 cut(s) 667, 687
BsaBI GATNNNNATC 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 460
BsaJI CCNNGG 3 cut(s) 52, 367, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 3 cut(s) 65, 425, 442
BseDI CCNNGG 3 cut(s) 52, 367, 403
BseGI GGATG 4 cut(s) 236, 538, 661, 883
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMII CTCAG 2 cut(s) 324, 540
BseRI GAGGAG 1 cut(s) 41
BseSI GKGCMC 2 cut(s) 397, 557
BshFI GGCC 4 cut(s) 366, 372, 547, 892
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 397, 557
BsiSI CCGG 2 cut(s) 666, 686
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 740
BsnI GGCC 4 cut(s) 366, 372, 547, 892
Bsp1286I GDGCHC 2 cut(s) 397, 557
Bsp143I GATC 4 cut(s) 8, 92, 231, 444
Bsp19I CCATGG 1 cut(s) 403
BspACI CCGC 1 cut(s) 463
BspANI GGCC 4 cut(s) 366, 372, 547, 892
BspCNI CTCAG 2 cut(s) 323, 541
BspHI TCATGA 2 cut(s) 673, 766
BspLI GGNNCC 2 cut(s) 150, 546
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 2 cut(s) 226, 452
BspT107I GGYRCC 1 cut(s) 148
BssECI CCNNGG 3 cut(s) 52, 367, 403
BssMI GATC 4 cut(s) 8, 92, 231, 444
BssNI GRCGYC 1 cut(s) 460
BssT1I CCWWGG 2 cut(s) 367, 403
Bst2UI CCWGG 3 cut(s) 65, 425, 442
BstACI GRCGYC 1 cut(s) 460
BstDEI CTNAG 2 cut(s) 310, 549
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 4 cut(s) 236, 538, 661, 883
BstKTI GATC 4 cut(s) 11, 95, 234, 447
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 4 cut(s) 8, 92, 231, 444
BstMWI GCNNNNNNNGC 1 cut(s) 378
BstNI CCWGG 3 cut(s) 65, 425, 442
BstNSI RCATGY 2 cut(s) 589, 829
BstSCI CCNGG 5 cut(s) 63, 423, 440, 665, 685
BstSFI CTRYAG 2 cut(s) 192, 558
BstSLI GKGCMC 2 cut(s) 397, 557
BstV2I GAAGAC 2 cut(s) 438, 795
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 4 cut(s) 366, 372, 547, 892
BtgI CCRYGG 1 cut(s) 403
BtsCI GGATG 4 cut(s) 236, 538, 661, 883
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 2 cut(s) 318, 555
CciI TCATGA 2 cut(s) 673, 766
Cfr13I GGNCC 1 cut(s) 545
CseI GACGC 1 cut(s) 468
Csp6I GTAC 3 cut(s) 149, 210, 580
CviAII CATG 9 cut(s) 345, 404, 488, 529, 586, 674, 755, 767, 826
CviQI GTAC 3 cut(s) 149, 210, 580
DdeI CTNAG 2 cut(s) 310, 549
DpnI GATC 4 cut(s) 10, 94, 233, 446
DpnII GATC 4 cut(s) 8, 92, 231, 444
EaeI YGGCCR 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 367, 403
EcoO109I RGGNCCY 1 cut(s) 545
EcoRII CCWGG 3 cut(s) 63, 423, 440
EcoT14I CCWWGG 2 cut(s) 367, 403
ErhI CCWWGG 2 cut(s) 367, 403
FaeI CATG 9 cut(s) 348, 407, 491, 532, 589, 677, 758, 770, 829
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FatI CATG 9 cut(s) 344, 403, 487, 528, 585, 673, 754, 766, 825
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 1 cut(s) 463
FokI GGATG 4 cut(s) 223, 545, 668, 890
Fsp4HI GCNGC 1 cut(s) 463
FspBI CTAG 4 cut(s) 245, 329, 867, 885
GluI GCNGC 1 cut(s) 463
HaeIII GGCC 4 cut(s) 366, 372, 547, 892
HapII CCGG 2 cut(s) 666, 686
HgaI GACGC 1 cut(s) 468
Hin1I GRCGYC 1 cut(s) 460
Hin1II CATG 9 cut(s) 348, 407, 491, 532, 589, 677, 758, 770, 829
HindIII AAGCTT 2 cut(s) 41, 97
HinfI GANTC 5 cut(s) 4, 113, 325, 437, 479
HpaII CCGG 2 cut(s) 666, 686
HphI GGTGA 6 cut(s) 3, 132, 195, 274, 333, 520
Hpy166II GTNNAC 4 cut(s) 395, 555, 611, 775
Hpy188I TCNGA 3 cut(s) 56, 112, 458
Hpy188III TCNNGA 4 cut(s) 88, 329, 674, 767
Hpy8I GTNNAC 4 cut(s) 395, 555, 611, 775
Hpy99I CGWCG 1 cut(s) 462
HpyAV CCTTC 5 cut(s) 174, 535, 598, 734, 833
HpyCH4V TGCA 7 cut(s) 225, 348, 395, 555, 560, 659, 829
HpyF10VI GCNNNNNNNGC 1 cut(s) 378
HpyF3I CTNAG 2 cut(s) 310, 549
Hsp92I GRCGYC 1 cut(s) 460
Hsp92II CATG 9 cut(s) 348, 407, 491, 532, 589, 677, 758, 770, 829
KpnI GGTACC 1 cut(s) 152
Kzo9I GATC 4 cut(s) 8, 92, 231, 444
LweI GCATC 1 cut(s) 646
MaeI CTAG 4 cut(s) 245, 329, 867, 885
MaeIII GTNAC 4 cut(s) 313, 490, 821, 869
MalI GATC 4 cut(s) 10, 94, 233, 446
MboI GATC 4 cut(s) 8, 92, 231, 444
MboII GAAGA 5 cut(s) 38, 440, 443, 702, 800
MfeI CAATTG 1 cut(s) 593
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 397, 557
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 3 cut(s) 124, 417, 593
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 2 cut(s) 13, 446
MmeI TCCRAC 1 cut(s) 481
Mox20I TGGCCA 1 cut(s) 372
MscI TGGCCA 1 cut(s) 372
MseI TTAA 2 cut(s) 179, 576
MslI CAYNNNNRTG 1 cut(s) 876
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 713
MspI CCGG 2 cut(s) 666, 686
MspR9I CCNGG 5 cut(s) 65, 425, 442, 667, 687
MunI CAATTG 1 cut(s) 593
MvaI CCWGG 3 cut(s) 65, 425, 442
MwoI GCNNNNNNNGC 1 cut(s) 378
NciI CCSGG 2 cut(s) 667, 687
NcoI CCATGG 1 cut(s) 403
NdeII GATC 4 cut(s) 8, 92, 231, 444
NlaIII CATG 9 cut(s) 348, 407, 491, 532, 589, 677, 758, 770, 829
NlaIV GGNNCC 2 cut(s) 150, 546
NmuCI GTSAC 3 cut(s) 313, 490, 869
NspI RCATGY 2 cut(s) 589, 829
PagI TCATGA 2 cut(s) 673, 766
PfeI GAWTC 3 cut(s) 113, 325, 479
PfoI TCCNGGA 2 cut(s) 423, 440
PkrI GCNGC 1 cut(s) 464
PleI GAGTC 2 cut(s) 12, 445
PpsI GAGTC 2 cut(s) 12, 445
Psp6I CCWGG 3 cut(s) 63, 423, 440
PspGI CCWGG 3 cut(s) 63, 423, 440
PspN4I GGNNCC 2 cut(s) 150, 546
PspPI GGNCC 1 cut(s) 545
PstI CTGCAG 1 cut(s) 562
PsuI RGATCY 1 cut(s) 231
PvuII CAGCTG 1 cut(s) 713
RsaI GTAC 3 cut(s) 150, 211, 581
RsaNI GTAC 3 cut(s) 149, 210, 580
RseI CAYNNNNRTG 1 cut(s) 876
SaqAI TTAA 2 cut(s) 179, 576
SatI GCNGC 1 cut(s) 463
Sau3AI GATC 4 cut(s) 8, 92, 231, 444
Sau96I GGNCC 1 cut(s) 545
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 2 cut(s) 13, 446
ScrFI CCNGG 5 cut(s) 65, 425, 442, 667, 687
SduI GDGCHC 2 cut(s) 397, 557
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 2 cut(s) 192, 558
SmiMI CAYNNNNRTG 1 cut(s) 876
SmlI CTYRAG 2 cut(s) 88, 299
SmoI CTYRAG 2 cut(s) 88, 299
Sse9I AATT 3 cut(s) 124, 417, 593
SsiI CCGC 1 cut(s) 463
SspI AATATT 1 cut(s) 217
SspMI CTAG 4 cut(s) 245, 329, 867, 885
StyD4I CCNGG 5 cut(s) 63, 423, 440, 665, 685
StyI CCWWGG 2 cut(s) 367, 403
TaqI TCGA 2 cut(s) 7, 447
TasI AATT 3 cut(s) 124, 417, 593
TatI WGTACW 2 cut(s) 209, 579
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 3 cut(s) 113, 325, 479
Tru1I TTAA 2 cut(s) 179, 576
Tru9I TTAA 2 cut(s) 179, 576
TscAI CASTG 2 cut(s) 318, 562
TseFI GTSAC 3 cut(s) 313, 490, 869
Tsp45I GTSAC 3 cut(s) 313, 490, 869
TspDTI ATGAA 3 cut(s) 117, 693, 755
TspRI CASTG 2 cut(s) 318, 562
VneI GTGCAC 2 cut(s) 393, 553
XapI RAATTY 1 cut(s) 124
XbaI TCTAGA 1 cut(s) 328
XceI RCATGY 2 cut(s) 589, 829
XmiI GTMKAC 1 cut(s) 610
XspI CTAG 4 cut(s) 245, 329, 867, 885
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.