FvH4_5g29050

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014160
Physical Location & Seq
Forward (+)
20157500 .. 20160139
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29050.t1

Sequence Viewer

Length: 2040 bp
ATGGCGGTTTCTCCACAAGCATTCCCGCCCAGAAAGCGGCGCCCCTCTGCCGGAGCCTTCATGTCTCCCAAACTCTCCGATCAGACCCTCCTCCGCTCTCTCCTCCTCCTCTGCCAAGAAATCTCCTCCCTCGAATCCCGCGGCTTCCTCCTCAGGCGCACCACCCTCTCCATGACTCGTAAATCCAAGCTTCTTGCTTTCCTTTTCGAAGACCTAGCTCGATCTTCAATCACCTACCTCTCTCCCTCCGCTCTCTTGTGCTTCCAAGAAATGTACATCCTCCTCCAGAGGATCCAAACCCTAATCGAGGACTCTTCCAACGGCAGCAAGATGTGGCTTCTCATCCAGAACGAGTCCCTCGCCAACAGCTTCCACGAGCTCACTCTTGATCTGTCCACCTTGCTAGATATTCTCCCCGTCAAGGAGCTTGACCTCAACGACGACGTCGAGGAAGTCGTCGCCTTGCTTCGCGCCCAGTGCGCCCGAAGCAAGGACGCTTTTGTTGATCCCAAGGATGACGACCTCCGCCTCCAAGTCTGCTACATGCTCGATCGTCTCAAAAAAGAAATCATTCCAGACCACTCCAAGCTCTCCGAGATTTTCGCCCAGTTGGGAATTCTAGACTCTTCCAATTGTAGAGAAGAGCTAGAGTTTCTTCAAGACGAGTTTCAGAACCAAGCAGACGACAAGTCCAAGTCTCAGTTGCTTTCCCTCATCGGCCTCGTTCGCTATGCCAAATGTGTCCTCTTCGGCGCTTCAACTCCACCTTCCGATCTCACTCGCCTGAAATCCTCCTCCGAGTTAAACTTCCCGGTGGACTTCCGGTGTCCGATCAGCCTAGAGCTCATGCGGGACCCGGTGGCCGTGGCGACCGGTCAGACCTACGATCGTGAGTCAATCAAGCTGTGGATCGAATCGGGACACAACACGTGTCCCAAGACGGGACAGGCCCTGGTCCACACGAACTTGATACCCAACCGCGCTTTGAAAAATCTTATCGCCATGTGGTGCCGCGAGCAGCGAATCCCTTTCGACCACGCGGAGAATAAGGAGAAAGTCAGCGCCGTCAAGAAGAACAAGGCCGCGTTGGAAGCCACCAGGATGACGGCCTCGTTTTTGGTCAACAAACTCTCCCTCGCCCAGTCAGTCGAGGCTGCCAACGGCGTCGTTTACGAGCTCCGTAGTCTAGCCAAGACTGATTCGGACTGCCGCGCCTGCATTGCCGAAGCTGGAGCGATTCCTTTGCTGGTACGCTATTTAGGCTCCGACGTCGGCAGGGACCACCCAAACCTTCAGGTAAACGCCGTCACAACGATTCTCAACCTCTCCATACTCGAAGCCAATAAAGCAAGAATCATGGACACTGACGGAGCGTTAAACGGAGTTATTGAGGTGCTCCGCTCTGGCGCCACGTGGGAGGCCAAGGGAAACGCGGCGGCTACGATATTCAGCTTGTCAGGTGTGCATGCGTACAGGAAGAAACTAGGGAGGAAGGCACGTGTGGTGAAGGCATTGGTGGAGTTGGCCAGGGCGGGACCCGGATTCTCCAAGAGGGACGCCATGGTGGCGATATTGAATTTGGCGGGAGAAAGAGATGCGGTGGCAAGGTTGGTGGAAGGCGGGGTGGTGGAGATGACGGTGGAAGTAATGGAGGAGCTACCGGAGGAGGCGGTGACGGTGTTGGAAGTGGTGGTGAGAAGCGGCGGGATGGTGGCCATTGCGGCGTCGTTTGGTGCAATACGGCATTTGGGGGTGGTGTTGAGAGAAGGGTCGGATAGGGCGAGGGAGAGCGCGGCGGCGACTCTTGTGACCATTTGTCGAAAAGGTGGGTCGGAGATGGTGGCGGAATTGGTGTCTGTTTCAGGGATAGAGAGGGTAATATGGGAATTGATGGGGACGGGGACGGTGAGGGCGAGGAGGAAAGCGGCGTCGTTGTTGAGGATTCTGAGGAGATGGGCGGCTGCAATGGAGGGTGATCATATAGTGGAAGGGTATTCGACTATTGTGAGTTCATCAACGACGGCAATGGTGTCTAGCTAA

Protein Analysis

680

Amino Acids

74.52

Weight (kDa)

7.9

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB10_N PF25368 52 - 164 1.9e-11 PUB10-like, N-terminal domain
U-box PF04564 271 - 341 1e-19 U-box domain
ARM_PUB PF25598 376 - 651 2.2e-15 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01830
fragaria_vesca FvH4_5g29050
malus_domestica MD04G1172400.v1.1 MD12G1185600.v1.1
prunus_persica Prupe.6G290800_v2.0.a1
pyrus_communis pycom04g15230 pycom12g17420
rosa_chinensis RchiOBHm_Chr3g0460861
rosa_laevigata RLG00000024973
rosa_multiflora Rmu_sc0009011.1_g000007
rosa_roxburghii Rroxscaffold_6G00419150
rosa_rugosa Rorug03G0047000
rosa_samantha Rh3AG104100 Rh3BG107800 Rh3CG109600 Rh3DG109600
rosa_wichuraiana Rw3G008860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 447, 1272
AccB1I GGYRCC 3 cut(s) 39, 1008, 1406
AccBSI CCGCTC 3 cut(s) 96, 251, 1401
AccII CGCG 9 cut(s) 141, 471, 981, 1014, 1040, 1085, 1212, 1433, 1793
AclWI GGATC 4 cut(s) 286, 299, 500, 917
AcoI YGGCCR 3 cut(s) 861, 1524, 1713
AcsI RAATTY 2 cut(s) 615, 1576
AcuI CTGAAG 1 cut(s) 1277
AcvI CACGTG 3 cut(s) 930, 1413, 1499
AcyI GRCGYC 8 cut(s) 40, 444, 1164, 1269, 1407, 1557, 1724, 1928
AfaI GTAC 3 cut(s) 275, 1251, 1472
AfiI CCNNNNNNNGG 6 cut(s) 36, 50, 307, 421, 490, 941
AflIII ACRYGT 3 cut(s) 927, 929, 1498
AgeI ACCGGT 1 cut(s) 872
AgsI TTSAA 5 cut(s) 228, 659, 759, 988, 1576
AhdI GACNNNNNGTC 2 cut(s) 688, 1815
AjnI CCWGG 3 cut(s) 951, 1097, 1526
Alw21I GWGCWC 4 cut(s) 381, 846, 1179, 1398
Alw26I GTCTC 3 cut(s) 69, 560, 702
AlwI GGATC 4 cut(s) 286, 299, 500, 917
AlwNI CAGNNNCTG 1 cut(s) 952
AoxI GGCC 8 cut(s) 718, 861, 948, 1080, 1107, 1419, 1524, 1713
ApeKI GCWGC 4 cut(s) 324, 1018, 1154, 1961
ApoI RAATTY 2 cut(s) 615, 1576
AsiGI ACCGGT 1 cut(s) 872
Asp700I GAANNNNTTC 1 cut(s) 570
AspS9I GGNCC 5 cut(s) 853, 949, 955, 1279, 1535
AsuC2I CCSGG 3 cut(s) 812, 857, 1539
AsuHPI GGTGA 6 cut(s) 223, 1516, 1684, 1705, 1918, 1985
AsuII TTCGAA 1 cut(s) 207
AvaII GGWCC 4 cut(s) 853, 955, 1279, 1535
AxyI CCTNAGG 1 cut(s) 152
BalI TGGCCA 2 cut(s) 1526, 1715
BamHI GGATCC 1 cut(s) 291
BanI GGYRCC 3 cut(s) 39, 1008, 1406
BanII GRGCYC 3 cut(s) 381, 846, 1179
BauI CACGAG 1 cut(s) 374
BbrPI CACGTG 3 cut(s) 930, 1413, 1499
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 4 cut(s) 381, 846, 1179, 1398
BbvI GCAGC 4 cut(s) 336, 1030, 1141, 1948
BccI CCATC 4 cut(s) 1702, 1831, 1885, 1947
BceAI ACGGC 8 cut(s) 337, 848, 1049, 1122, 1177, 1289, 1757, 2037
BcgI CGANNNNNNTGC 2 cut(s) 1225, 1259
BciT130I CCWGG 3 cut(s) 953, 1099, 1528
BclI TGATCA 1 cut(s) 1975
BcnI CCSGG 3 cut(s) 812, 857, 1539
BcoDI GTCTC 3 cut(s) 69, 560, 702
BfaI CTAG 8 cut(s) 215, 404, 620, 647, 839, 1187, 1484, 2034
BfoI RGCGCY 4 cut(s) 43, 756, 1065, 1410
BglI GCCNNNNNGGC 2 cut(s) 1565, 1721
Bme1390I CCNGG 6 cut(s) 812, 857, 953, 1099, 1528, 1539
Bme18I GGWCC 4 cut(s) 853, 955, 1279, 1535
BmeRI GACNNNNNGTC 2 cut(s) 688, 1815
BmgT120I GGNCC 5 cut(s) 853, 949, 955, 1279, 1535
BmrFI CCNGG 6 cut(s) 812, 857, 953, 1099, 1528, 1539
BmrI ACTGGG 3 cut(s) 469, 601, 1135
BmsI GCATC 1 cut(s) 1585
BmuI ACTGGG 3 cut(s) 469, 601, 1135
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 2 cut(s) 269, 1251
Bpu14I TTCGAA 1 cut(s) 207
BpuMI CCSGG 3 cut(s) 812, 857, 1539
BsaAI YACGTR 3 cut(s) 930, 1413, 1499
BsaHI GRCGYC 8 cut(s) 40, 444, 1164, 1269, 1407, 1557, 1724, 1928
BsaJI CCNNGG 7 cut(s) 139, 510, 864, 951, 1422, 1527, 1560
BsaWI WCCGGW 3 cut(s) 822, 872, 1660
BsaXI ACNNNNNCTCC 2 cut(s) 1115, 1145
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 50, 307, 421, 490, 941
Bse118I RCCGGY 1 cut(s) 872
Bse1I ACTGG 3 cut(s) 475, 607, 1141
Bse21I CCTNAGG 1 cut(s) 152
Bse3DI GCAATG 4 cut(s) 1218, 1716, 1971, 2031
BseBI CCWGG 3 cut(s) 953, 1099, 1528
BseDI CCNNGG 7 cut(s) 139, 510, 864, 951, 1422, 1527, 1560
BseGI GGATG 5 cut(s) 276, 342, 520, 1107, 1713
BseLI CCNNNNNNNGG 6 cut(s) 36, 50, 307, 421, 490, 941
BseMI GCAATG 4 cut(s) 1218, 1716, 1971, 2031
BseMII CTCAG 3 cut(s) 166, 713, 1937
BseNI ACTGG 3 cut(s) 475, 607, 1141
BseXI GCAGC 4 cut(s) 336, 1030, 1141, 1948
Bsh1236I CGCG 9 cut(s) 141, 471, 981, 1014, 1040, 1085, 1212, 1433, 1793
Bsh1285I CGRYCG 3 cut(s) 553, 873, 889
BshFI GGCC 8 cut(s) 720, 863, 950, 1082, 1109, 1421, 1526, 1715
BshNI GGYRCC 3 cut(s) 39, 1008, 1406
BshTI ACCGGT 1 cut(s) 872
BsiEI CGRYCG 3 cut(s) 553, 873, 889
BsiHKAI GWGCWC 4 cut(s) 381, 846, 1179, 1398
BsiSI CCGG 7 cut(s) 51, 812, 823, 857, 873, 1539, 1661
BslI CCNNNNNNNGG 6 cut(s) 36, 50, 307, 421, 490, 941
BsmAI GTCTC 3 cut(s) 69, 560, 702
BsmBI CGTCTC 1 cut(s) 560
BsmI GAATGC 1 cut(s) 20
BsnI GGCC 8 cut(s) 720, 863, 950, 1082, 1109, 1421, 1526, 1715
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 4 cut(s) 381, 846, 1179, 1398
Bsp1407I TGTACA 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 1560
BspANI GGCC 8 cut(s) 720, 863, 950, 1082, 1109, 1421, 1526, 1715
BspCNI CTCAG 3 cut(s) 165, 712, 1938
BspFNI CGCG 9 cut(s) 141, 471, 981, 1014, 1040, 1085, 1212, 1433, 1793
BspPI GGATC 4 cut(s) 286, 299, 500, 917
BspQI GCTCTTC 1 cut(s) 636
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 3 cut(s) 39, 1008, 1406
BsrBI CCGCTC 3 cut(s) 96, 251, 1401
BsrDI GCAATG 4 cut(s) 1218, 1716, 1971, 2031
BsrFI RCCGGY 1 cut(s) 872
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 3 cut(s) 475, 607, 1141
BssAI RCCGGY 1 cut(s) 872
BssECI CCNNGG 7 cut(s) 139, 510, 864, 951, 1422, 1527, 1560
BssNI GRCGYC 8 cut(s) 40, 444, 1164, 1269, 1407, 1557, 1724, 1928
BssSI CACGAG 1 cut(s) 374
BssT1I CCWWGG 3 cut(s) 510, 1422, 1560
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 3 cut(s) 953, 1099, 1528
Bst4CI ACNGT 3 cut(s) 1639, 1678, 1906
Bst6I CTCTTC 4 cut(s) 319, 631, 636, 752
BstACI GRCGYC 8 cut(s) 40, 444, 1164, 1269, 1407, 1557, 1724, 1928
BstAUI TGTACA 1 cut(s) 273
BstBAI YACGTR 3 cut(s) 930, 1413, 1499
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 3 cut(s) 1016, 1216, 1467
BstDEI CTNAG 3 cut(s) 152, 699, 1946
BstDSI CCRYGG 3 cut(s) 139, 864, 1560
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 5 cut(s) 276, 342, 520, 1107, 1713
BstFNI CGCG 9 cut(s) 141, 471, 981, 1014, 1040, 1085, 1212, 1433, 1793
BstH2I RGCGCY 4 cut(s) 43, 756, 1065, 1410
BstMAI GTCTC 3 cut(s) 69, 560, 702
BstMCI CGRYCG 3 cut(s) 553, 873, 889
BstNI CCWGG 3 cut(s) 953, 1099, 1528
BstNSI RCATGY 2 cut(s) 547, 1469
BstSCI CCNGG 6 cut(s) 810, 855, 951, 1097, 1526, 1537
BstUI CGCG 9 cut(s) 141, 471, 981, 1014, 1040, 1085, 1212, 1433, 1793
BstV1I GCAGC 4 cut(s) 336, 1030, 1141, 1948
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
Bsu36I CCTNAGG 1 cut(s) 152
BsuRI GGCC 8 cut(s) 720, 863, 950, 1082, 1109, 1421, 1526, 1715
BtgI CCRYGG 3 cut(s) 139, 864, 1560
BtsCI GGATG 5 cut(s) 276, 342, 520, 1107, 1713
BtsIMutI CAGTG 2 cut(s) 482, 1362
Cac8I GCNNGC 3 cut(s) 1016, 1216, 1467
CaiI CAGNNNCTG 1 cut(s) 952
Cfr10I RCCGGY 1 cut(s) 872
Cfr13I GGNCC 5 cut(s) 853, 949, 955, 1279, 1535
Cfr42I CCGCGG 1 cut(s) 142
CseI GACGC 5 cut(s) 503, 1153, 1565, 1713, 1917
Csp6I GTAC 3 cut(s) 274, 1250, 1471
CspAI ACCGGT 1 cut(s) 872
CspCI CAANNNNNGTGG 2 cut(s) 1593, 1628
CviAII CATG 8 cut(s) 61, 172, 544, 847, 1003, 1357, 1466, 1561
CviQI GTAC 3 cut(s) 274, 1250, 1471
DdeI CTNAG 3 cut(s) 152, 699, 1946
DinI GGCGCC 2 cut(s) 41, 1408
DriI GACNNNNNGTC 2 cut(s) 688, 1815
EaeI YGGCCR 3 cut(s) 861, 1524, 1713
Eam1104I CTCTTC 4 cut(s) 319, 631, 636, 752
Eam1105I GACNNNNNGTC 2 cut(s) 688, 1815
EarI CTCTTC 4 cut(s) 319, 631, 636, 752
EciI GGCGGA 2 cut(s) 515, 1859
Ecl136II GAGCTC 3 cut(s) 379, 844, 1177
Eco130I CCWWGG 3 cut(s) 510, 1422, 1560
Eco24I GRGCYC 3 cut(s) 381, 846, 1179
Eco47I GGWCC 4 cut(s) 853, 955, 1279, 1535
Eco53kI GAGCTC 3 cut(s) 379, 844, 1177
Eco57I CTGAAG 1 cut(s) 1277
Eco72I CACGTG 3 cut(s) 930, 1413, 1499
Eco81I CCTNAGG 1 cut(s) 152
EcoICRI GAGCTC 3 cut(s) 379, 844, 1177
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO109I RGGNCCY 3 cut(s) 853, 949, 1535
EcoRI GAATTC 1 cut(s) 615
EcoRII CCWGG 3 cut(s) 951, 1097, 1526
EcoT14I CCWWGG 3 cut(s) 510, 1422, 1560
EcoT38I GRGCYC 3 cut(s) 381, 846, 1179
EgeI GGCGCC 2 cut(s) 41, 1408
EheI GGCGCC 2 cut(s) 41, 1408
ErhI CCWWGG 3 cut(s) 510, 1422, 1560
Esp3I CGTCTC 1 cut(s) 560
FaeI CATG 8 cut(s) 64, 175, 547, 850, 1006, 1360, 1469, 1564
FatI CATG 8 cut(s) 60, 171, 543, 846, 1002, 1356, 1465, 1560
FauI CCCGC 7 cut(s) 33, 146, 843, 1525, 1576, 1613, 1697
FbaI TGATCA 1 cut(s) 1975
FokI GGATG 5 cut(s) 263, 329, 527, 1114, 1720
FriOI GRGCYC 3 cut(s) 381, 846, 1179
FspBI CTAG 8 cut(s) 215, 404, 620, 647, 839, 1187, 1484, 2034
GsuI CTGGAG 2 cut(s) 269, 1251
HaeII RGCGCY 4 cut(s) 43, 756, 1065, 1410
HaeIII GGCC 8 cut(s) 720, 863, 950, 1082, 1109, 1421, 1526, 1715
HapII CCGG 7 cut(s) 51, 812, 823, 857, 873, 1539, 1661
HgaI GACGC 5 cut(s) 503, 1153, 1565, 1713, 1917
Hin1I GRCGYC 8 cut(s) 40, 444, 1164, 1269, 1407, 1557, 1724, 1928
Hin1II CATG 8 cut(s) 64, 175, 547, 850, 1006, 1360, 1469, 1564
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HindIII AAGCTT 1 cut(s) 188
HpaII CCGG 7 cut(s) 51, 812, 823, 857, 873, 1539, 1661
HphI GGTGA 6 cut(s) 223, 1516, 1684, 1705, 1918, 1985
Hpy166II GTNNAC 6 cut(s) 396, 817, 958, 1123, 1171, 1300
Hpy188III TCNNGA 9 cut(s) 286, 346, 386, 575, 620, 659, 890, 918, 1069
Hpy8I GTNNAC 6 cut(s) 396, 817, 958, 1123, 1171, 1300
HpyAV CCTTC 8 cut(s) 67, 777, 1301, 1486, 1501, 1610, 1760, 1982
HpyCH4III ACNGT 3 cut(s) 1639, 1678, 1906
HpyCH4IV ACGT 5 cut(s) 444, 929, 1269, 1412, 1498
HpyCH4V TGCA 4 cut(s) 1218, 1465, 1736, 1964
HpyF3I CTNAG 3 cut(s) 152, 699, 1946
HpySE526I ACGT 5 cut(s) 444, 929, 1269, 1412, 1498
Hsp92I GRCGYC 8 cut(s) 40, 444, 1164, 1269, 1407, 1557, 1724, 1928
Hsp92II CATG 8 cut(s) 64, 175, 547, 850, 1006, 1360, 1469, 1564
KasI GGCGCC 2 cut(s) 39, 1406
KflI GGGWCCC 2 cut(s) 853, 1535
Ksp22I TGATCA 1 cut(s) 1975
KspI CCGCGG 1 cut(s) 142
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 8 cut(s) 53, 424, 1182, 1232, 1268, 1370, 1401, 1654
Lsp1109I GCAGC 4 cut(s) 336, 1030, 1141, 1948
LweI GCATC 1 cut(s) 1585
MaeI CTAG 8 cut(s) 215, 404, 620, 647, 839, 1187, 1484, 2034
MaeII ACGT 5 cut(s) 444, 929, 1269, 1412, 1498
MaeIII GTNAC 3 cut(s) 1306, 1672, 1807
MbiI CCGCTC 3 cut(s) 96, 251, 1401
MboII GAAGA 9 cut(s) 216, 221, 306, 618, 647, 653, 739, 1084, 1489
MfeI CAATTG 1 cut(s) 631
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 4 cut(s) 381, 846, 1179, 1398
MlsI TGGCCA 2 cut(s) 1526, 1715
MluCI AATT 5 cut(s) 615, 631, 1576, 1847, 1886
MluNI TGGCCA 2 cut(s) 1526, 1715
Mly113I GGCGCC 2 cut(s) 40, 1407
MlyI GAGTC 6 cut(s) 169, 305, 362, 617, 902, 1795
MmeI TCCRAC 6 cut(s) 342, 1068, 1290, 1662, 1752, 1812
Mox20I TGGCCA 2 cut(s) 1526, 1715
MroXI GAANNNNTTC 1 cut(s) 570
MscI TGGCCA 2 cut(s) 1526, 1715
MseI TTAA 2 cut(s) 803, 1376
MslI CAYNNNNRTG 1 cut(s) 1100
Msp20I TGGCCA 2 cut(s) 1526, 1715
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 7 cut(s) 51, 812, 823, 857, 873, 1539, 1661
MspR9I CCNGG 6 cut(s) 812, 857, 953, 1099, 1528, 1539
MunI CAATTG 1 cut(s) 631
Mva1269I GAATGC 1 cut(s) 20
MvaI CCWGG 3 cut(s) 953, 1099, 1528
MvnI CGCG 9 cut(s) 141, 471, 981, 1014, 1040, 1085, 1212, 1433, 1793
NarI GGCGCC 2 cut(s) 40, 1407
NciI CCSGG 3 cut(s) 812, 857, 1539
NcoI CCATGG 1 cut(s) 1560
NlaIII CATG 8 cut(s) 64, 175, 547, 850, 1006, 1360, 1469, 1564
NmuCI GTSAC 3 cut(s) 1306, 1672, 1807
NspI RCATGY 2 cut(s) 547, 1469
NspV TTCGAA 1 cut(s) 207
PaeI GCATGC 1 cut(s) 1469
PciSI GCTCTTC 1 cut(s) 636
PcsI WCGNNNNNNNCGW 4 cut(s) 444, 453, 1778, 1910
PctI GAATGC 1 cut(s) 20
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 9 cut(s) 134, 914, 1023, 1199, 1237, 1315, 1353, 1542, 1942
PflFI GACNNNGTC 1 cut(s) 443
PinAI ACCGGT 1 cut(s) 872
Ple19I CGATCG 2 cut(s) 553, 889
PleI GAGTC 6 cut(s) 169, 305, 361, 617, 901, 1795
PluTI GGCGCC 2 cut(s) 43, 1410
PmaCI CACGTG 3 cut(s) 930, 1413, 1499
PmlI CACGTG 3 cut(s) 930, 1413, 1499
PpsI GAGTC 6 cut(s) 169, 305, 361, 617, 901, 1795
Ppu21I YACGTR 3 cut(s) 930, 1413, 1499
PpuMI RGGWCCY 2 cut(s) 853, 1535
Psp124BI GAGCTC 3 cut(s) 381, 846, 1179
Psp5II RGGWCCY 2 cut(s) 853, 1535
Psp6I CCWGG 3 cut(s) 951, 1097, 1526
PspCI CACGTG 3 cut(s) 930, 1413, 1499
PspGI CCWGG 3 cut(s) 951, 1097, 1526
PspPI GGNCC 5 cut(s) 853, 949, 955, 1279, 1535
PspPPI RGGWCCY 2 cut(s) 853, 1535
PstNI CAGNNNCTG 1 cut(s) 952
PsuI RGATCY 1 cut(s) 291
PsyI GACNNNGTC 1 cut(s) 443
PvuI CGATCG 2 cut(s) 553, 889
RsaI GTAC 3 cut(s) 275, 1251, 1472
RsaNI GTAC 3 cut(s) 274, 1250, 1471
RseI CAYNNNNRTG 1 cut(s) 1100
SacI GAGCTC 3 cut(s) 381, 846, 1179
SacII CCGCGG 1 cut(s) 142
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 2 cut(s) 803, 1376
Sau96I GGNCC 5 cut(s) 853, 949, 955, 1279, 1535
SchI GAGTC 6 cut(s) 169, 305, 362, 617, 902, 1795
ScrFI CCNGG 6 cut(s) 812, 857, 953, 1099, 1528, 1539
SduI GDGCHC 4 cut(s) 381, 846, 1179, 1398
SfaNI GCATC 1 cut(s) 1585
SfoI GGCGCC 2 cut(s) 41, 1408
Sfr303I CCGCGG 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 207
SgrBI CCGCGG 1 cut(s) 142
SinI GGWCC 4 cut(s) 853, 955, 1279, 1535
SmiMI CAYNNNNRTG 1 cut(s) 1100
SphI GCATGC 1 cut(s) 1469
Sse9I AATT 5 cut(s) 615, 631, 1576, 1847, 1886
SspDI GGCGCC 2 cut(s) 39, 1406
SspMI CTAG 8 cut(s) 215, 404, 620, 647, 839, 1187, 1484, 2034
SstI GAGCTC 3 cut(s) 381, 846, 1179
StyD4I CCNGG 6 cut(s) 810, 855, 951, 1097, 1526, 1537
StyI CCWWGG 3 cut(s) 510, 1422, 1560
TaaI ACNGT 3 cut(s) 1639, 1678, 1906
TaiI ACGT 5 cut(s) 447, 932, 1272, 1415, 1501
TasI AATT 5 cut(s) 615, 631, 1576, 1847, 1886
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 9 cut(s) 134, 914, 1023, 1199, 1237, 1315, 1353, 1542, 1942
Tru1I TTAA 2 cut(s) 803, 1376
Tru9I TTAA 2 cut(s) 803, 1376
TscAI CASTG 2 cut(s) 482, 1369
TseFI GTSAC 3 cut(s) 1306, 1672, 1807
TseI GCWGC 4 cut(s) 324, 1018, 1154, 1961
Tsp45I GTSAC 3 cut(s) 1306, 1672, 1807
TspDTI ATGAA 2 cut(s) 49, 2001
TspGWI ACGGA 3 cut(s) 1169, 1383, 1395
TspRI CASTG 2 cut(s) 482, 1369
Tth111I GACNNNGTC 1 cut(s) 443
VpaK11BI GGWCC 4 cut(s) 853, 955, 1279, 1535
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 2 cut(s) 615, 1576
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 2 cut(s) 547, 1469
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 8 cut(s) 215, 404, 620, 647, 839, 1187, 1484, 2034
ZraI GACGTC 2 cut(s) 445, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.