Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0014160

Physical Location & Seq

Genomic Coordinates Forward (+)

19789358 .. 19791112

Gene Structure 1755 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g17420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGGAAGATTCCTCCAACGGGAGCCGGATGTGGCTCCTCCTCCAGACCGAGTCCCTGGCCAACAGCTTCCACGAGCTCACCCTCGACCTCTCCACCTTGCTTGACATCCTCCCCGTCAAGGAGCTCCGATTAAACGACGATGTCGGGGAGGTCGTCTCCCTCCTCCGCAGCCAGTGCTCCCGCAGCACCACAAAATCGCTCGTCGATCCGAGAGACGACGCCCTCTGCCGCGAGGTTTGCTCGATGCTCGACCGCCTCAGGAGCGAGATCGCCCCCGACCACTCCAAGCTCTCTGACATTTTCGCGCAATTAGGGATTCGCGACGCTTCGAATTGCAGAGAGGAGCTCGAGAGCCTCCAGGACGAGTTCCAGAACCAGACGGAGGAGAAATCCAAGTCGCAGCTCATCGCCCTCGTCGGACTCGTTCGCTACGGCAAGTGCGTGTTATTTGGAGCATCCACACCGGGATCGGACCTAATCCGCCGTAGATCGGCATCGGAATTAAGTTTCCCGGTGGATTTTCGGTGCCCGATCAGTTTGGAGCTAATGCGGGACCCGGTCACCGTGGCCACCGGCCAGACATACGATCGTGATTCCATCAAGCTGTGGATTGAATCAGGACACAACACGTGTCCCAAGACAGGTCAGGCCCTGGCCCACACGGAGCTCATACCGAATCGCGCGTTGAAAAATCTCATCGCCATGTGGTGCCGCGAGCAGAGAGTCCCTTTCGAAACCGCGGAGAACAATAAGGGGCTGGTCAACGCCGTCAAAAAGAACAAGGCGGCGCTAGAAGCTACGCGGATGACGGGCTCGTTTTTGGTCAACAAGCTTTCGGTGTCTCAGTCGATGGAGGCGGCGAACGGCGTCGTTTACGAGCTCCGGAGTCTGGCGAAGACGGACTCCGACTGTCGCGCCTGCATTGCGGAGGCCGGGGCTATTCCTTTGCTGGTACGGTACTTAAGCTCGGACGTGGGAGAGACCCATCCGAACCTGCAGGTTAACGCCGTGACGACGATTCTTAACCTTTCTATTTTGGAAGCGAACAAAACGCGGATCATGGAGACTGACGGAGCTCTTAACGGCGTTATCGAGGTGCTGAGGTCGGGGGCCACCTGGGAGGCGAAGGGGAATGCAGCGGCTACGATATTCAGTTTATCAGGTGTGCACGCGTACAGGAAGAGACTCGGGAAGAAGACACGTGTCATAAAGGGGTTAGTGGAGCTGGCGAGGGATGGGCCCGAGCGCGGGTGTTTTAAAAAGGACGCCATGGTGGCGATACTGAATTTGGCGGGAGAGAGGGAGGCAGTGGCGAGATTGGTGGAAGGCGGGGTGGTGGAGATGACCGTGCAAGTGATGGAGGGGCTGCCGGAGGAGGCGGTTACTGTGTTGGAAATTGTGGTGAGAAGCGGCGGACTACTAGCCATTGCGGCGGCATATCGCGCGATTAGAAAATTGGGGAGTGTGTTGAGAGAAGGGTCGGAGAGGGCGAGGGAGAGCGCGGCGGCCACGCTGGTGACAATCTGTCGAAAAGGGGGATCGGAGATGGTGGCGGAATTGGCGTCAATGCCGGGGATTGAGAGGGTGATATGGGAATTGATGGGGGTGGGGACGGTGAGGGCGAGGCGGAAGGCGGCGTCTCTATTGAGGATTCTGAGGAGATGGGCTGCCGGTTTGGATAGTGAGGTAGCGGAGGGGGGATATTCAACGGTGAATCATGTGAGTACATCGGCAACAACAGTAGTGGCAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

585

Amino Acids

63.52

Weight (kDa)

7.05

Isoelectric Point (pI)

39.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	171 - 241	5.8e-20	U-box domain
ARM_ARMC5	PF24768	272 - 439	4.9e-06	ARMC5
ARM_PUB	PF25598	275 - 554	1.5e-17	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014160)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01830
fragaria_vesca	FvH4_5g29050
malus_domestica	MD04G1172400.v1.1 MD12G1185600.v1.1
prunus_persica	Prupe.6G290800_v2.0.a1
pyrus_communis	pycom04g15230 pycom12g17420
rosa_chinensis	RchiOBHm_Chr3g0460861
rosa_laevigata	RLG00000024973
rosa_multiflora	Rmu_sc0009011.1_g000007
rosa_roxburghii	Rroxscaffold_6G00419150
rosa_rugosa	Rorug03G0047000
rosa_samantha	Rh3AG104100 Rh3BG107800 Rh3CG109600 Rh3DG109600
rosa_wichuraiana	Rw3G008860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	988, 1002
AccB1I	GGYRCC	2 cut(s)	525, 708
AccIII	TCCGGA	1 cut(s)	882
AclWI	GGATC	4 cut(s)	200, 475, 1064, 1546
AcoI	YGGCCR	4 cut(s)	57, 567, 574, 1506
AcsI	RAATTY	1 cut(s)	1285
AcvI	CACGTG	2 cut(s)	630, 1202
AcyI	GRCGYC	5 cut(s)	219, 867, 1266, 1562, 1637
AfaI	GTAC	4 cut(s)	954, 959, 1175, 1726
AfiI	CCNNNNNNNGG	8 cut(s)	18, 118, 382, 490, 641, 889, 1247, 1248
AflII	CTTAAG	1 cut(s)	961
AflIII	ACRYGT	5 cut(s)	627, 629, 1170, 1199, 1201
AgsI	TTSAA	3 cut(s)	614, 688, 1707
AhdI	GACNNNNNGTC	1 cut(s)	1524
AjiI	CACGTC	1 cut(s)	973
AjnI	CCWGG	4 cut(s)	54, 357, 651, 1115
AleI	CACNNNNGTG	1 cut(s)	1514
Alw21I	GWGCWC	8 cut(s)	78, 126, 179, 348, 669, 882, 1078, 1170
Alw26I	GTCTC	7 cut(s)	160, 207, 846, 974, 1058, 1177, 1644
Alw44I	GTGCAC	1 cut(s)	1166
AlwI	GGATC	4 cut(s)	200, 475, 1064, 1546
AlwNI	CAGNNNCTG	1 cut(s)	652
Ama87I	CYCGRG	3 cut(s)	347, 1187, 1241
Aor13HI	TCCGGA	1 cut(s)	882
AoxI	GGCC	9 cut(s)	57, 567, 574, 648, 654, 930, 1110, 1238, 1506
ApaI	GGGCCC	1 cut(s)	1242
ApaLI	GTGCAC	1 cut(s)	1166
ApeKI	GCWGC	6 cut(s)	168, 183, 400, 1136, 1366, 1667
ApoI	RAATTY	1 cut(s)	1285
AspLEI	GCGC	7 cut(s)	307, 683, 790, 917, 1248, 1445, 1502
AspS9I	GGNCC	7 cut(s)	472, 553, 649, 655, 1110, 1238, 1239
AsuC2I	CCSGG	5 cut(s)	465, 512, 557, 934, 1572
AsuHPI	GGTGA	7 cut(s)	70, 553, 1414, 1528, 1597, 1627, 1723
AsuII	TTCGAA	2 cut(s)	329, 732
AvaI	CYCGRG	3 cut(s)	347, 1187, 1241
AvaII	GGWCC	2 cut(s)	472, 553
AxyI	CCTNAGG	1 cut(s)	257
BaeGI	GKGCMC	3 cut(s)	530, 1170, 1242
BalI	TGGCCA	2 cut(s)	59, 569
BanI	GGYRCC	2 cut(s)	525, 708
BanII	GRGCYC	8 cut(s)	78, 126, 348, 669, 815, 882, 1078, 1242
BauI	CACGAG	1 cut(s)	71
BbrPI	CACGTG	2 cut(s)	630, 1202
BbsI	GAAGAC	2 cut(s)	902, 1202
Bbv12I	GWGCWC	8 cut(s)	78, 126, 179, 348, 669, 882, 1078, 1170
BbvCI	CCTCAGC	1 cut(s)	1100
BbvI	GCAGC	6 cut(s)	180, 195, 412, 1148, 1353, 1654
BccI	CCATC	8 cut(s)	605, 844, 993, 1229, 1351, 1540, 1594, 1656
BceAI	ACGGC	6 cut(s)	448, 468, 752, 880, 992, 1099
BciT130I	CCWGG	4 cut(s)	56, 359, 653, 1117
BcnI	CCSGG	5 cut(s)	465, 512, 557, 934, 1572
BcoDI	GTCTC	7 cut(s)	160, 207, 846, 974, 1058, 1177, 1644
BfaI	CTAG	2 cut(s)	791, 1421
BfmI	CTRYAG	1 cut(s)	995
BfoI	RGCGCY	1 cut(s)	791
BfrI	CTTAAG	1 cut(s)	961
BfuAI	ACCTGC	2 cut(s)	988, 1002
BglI	GCCNNNNNGGC	2 cut(s)	1274, 1430
Bme1390I	CCNGG	9 cut(s)	56, 359, 465, 512, 557, 653, 934, 1117, 1572
Bme18I	GGWCC	2 cut(s)	472, 553
BmeRI	GACNNNNNGTC	1 cut(s)	1524
BmeT110I	CYCGRG	3 cut(s)	347, 1187, 1241
BmgBI	CACGTC	1 cut(s)	973
BmgT120I	GGNCC	7 cut(s)	472, 553, 649, 655, 1110, 1238, 1239
BmiI	GGNNCC	8 cut(s)	23, 35, 527, 554, 555, 710, 1111, 1240
BmrFI	CCNGG	9 cut(s)	56, 359, 465, 512, 557, 653, 934, 1117, 1572
BmsI	GCATC	3 cut(s)	234, 464, 503
BoxI	GACNNNNGTC	1 cut(s)	1202
BpiI	GAAGAC	2 cut(s)	902, 1202
BplI	GAGNNNNNCTC	6 cut(s)	140, 172, 224, 256, 330, 362
BpmI	CTGGAG	2 cut(s)	26, 341
Bpu10I	CCTNAGC	1 cut(s)	1100
Bpu14I	TTCGAA	2 cut(s)	329, 732
BpuMI	CCSGG	5 cut(s)	465, 512, 557, 934, 1572
BsaAI	YACGTR	2 cut(s)	630, 1202
BsaBI	GATNNNNATC	1 cut(s)	493
BsaHI	GRCGYC	5 cut(s)	219, 867, 1266, 1562, 1637
BsaI	GGTCTC	1 cut(s)	974
BsaJI	CCNNGG	8 cut(s)	54, 564, 651, 738, 933, 1116, 1269, 1571
BsaWI	WCCGGW	1 cut(s)	882
Bsc4I	CCNNNNNNNGG	8 cut(s)	18, 118, 382, 490, 641, 889, 1247, 1248
Bse118I	RCCGGY	2 cut(s)	572, 1670
Bse1I	ACTGG	1 cut(s)	172
Bse21I	CCTNAGG	1 cut(s)	257
Bse3DI	GCAATG	2 cut(s)	921, 1425
Bse8I	GATNNNNATC	1 cut(s)	493
BseAI	TCCGGA	1 cut(s)	882
BseBI	CCWGG	4 cut(s)	56, 359, 653, 1117
BseDI	CCNNGG	8 cut(s)	54, 564, 651, 738, 933, 1116, 1269, 1571
BseGI	GGATG	6 cut(s)	33, 105, 455, 810, 985, 1240
BseJI	GATNNNNATC	1 cut(s)	493
BseLI	CCNNNNNNNGG	8 cut(s)	18, 118, 382, 490, 641, 889, 1247, 1248
BseMI	GCAATG	2 cut(s)	921, 1425
BseMII	CTCAG	4 cut(s)	271, 857, 1091, 1646
BseNI	ACTGG	1 cut(s)	172
BseRI	GAGGAG	7 cut(s)	26, 29, 152, 356, 398, 1388, 1672
BseSI	GKGCMC	3 cut(s)	530, 1170, 1242
BseXI	GCAGC	6 cut(s)	180, 195, 412, 1148, 1353, 1654
Bsh1285I	CGRYCG	2 cut(s)	253, 589
BshFI	GGCC	9 cut(s)	59, 569, 576, 650, 656, 932, 1112, 1240, 1508
BshNI	GGYRCC	2 cut(s)	525, 708
BsiEI	CGRYCG	2 cut(s)	253, 589
BsiHKAI	GWGCWC	8 cut(s)	78, 126, 179, 348, 669, 882, 1078, 1170
BsiHKCI	CYCGRG	3 cut(s)	347, 1187, 1241
BslFI	GGGAC	5 cut(s)	37, 566, 618, 710, 1624
BslI	CCNNNNNNNGG	8 cut(s)	18, 118, 382, 490, 641, 889, 1247, 1248
BsmAI	GTCTC	7 cut(s)	160, 207, 846, 974, 1058, 1177, 1644
BsmBI	CGTCTC	3 cut(s)	160, 207, 1644
BsmFI	GGGAC	5 cut(s)	37, 566, 618, 710, 1624
BsmI	GAATGC	1 cut(s)	1138
BsnI	GGCC	9 cut(s)	59, 569, 576, 650, 656, 932, 1112, 1240, 1508
Bso31I	GGTCTC	1 cut(s)	974
BsoBI	CYCGRG	3 cut(s)	347, 1187, 1241
Bsp119I	TTCGAA	2 cut(s)	329, 732
Bsp120I	GGGCCC	1 cut(s)	1238
Bsp13I	TCCGGA	1 cut(s)	882
Bsp143I	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
Bsp19I	CCATGG	1 cut(s)	1269
Bsp68I	TCGCGA	1 cut(s)	321
BspANI	GGCC	9 cut(s)	59, 569, 576, 650, 656, 932, 1112, 1240, 1508
BspCNI	CTCAG	4 cut(s)	270, 856, 1092, 1647
BspEI	TCCGGA	1 cut(s)	882
BspLI	GGNNCC	8 cut(s)	23, 35, 527, 554, 555, 710, 1111, 1240
BspMAI	CTGCAG	1 cut(s)	999
BspMI	ACCTGC	2 cut(s)	988, 1002
BspPI	GGATC	4 cut(s)	200, 475, 1064, 1546
BspT104I	TTCGAA	2 cut(s)	329, 732
BspT107I	GGYRCC	2 cut(s)	525, 708
BspTI	CTTAAG	1 cut(s)	961
BspTNI	GGTCTC	1 cut(s)	974
BsrDI	GCAATG	2 cut(s)	921, 1425
BsrFI	RCCGGY	2 cut(s)	572, 1670
BsrI	ACTGG	1 cut(s)	172
BssAI	RCCGGY	2 cut(s)	572, 1670
BssECI	CCNNGG	8 cut(s)	54, 564, 651, 738, 933, 1116, 1269, 1571
BssMI	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
BssNI	GRCGYC	5 cut(s)	219, 867, 1266, 1562, 1637
BssSI	CACGAG	1 cut(s)	71
BssT1I	CCWWGG	1 cut(s)	1269
Bst2BI	CACGAG	1 cut(s)	71
Bst2UI	CCWGG	4 cut(s)	56, 359, 653, 1117
Bst4CI	ACNGT	8 cut(s)	565, 911, 957, 1348, 1387, 1615, 1711, 1741
Bst6I	CTCTTC	1 cut(s)	1175
BstACI	GRCGYC	5 cut(s)	219, 867, 1266, 1562, 1637
BstAFI	CTTAAG	1 cut(s)	961
BstAPI	GCANNNNNTGC	1 cut(s)	174
BstBAI	YACGTR	2 cut(s)	630, 1202
BstBI	TTCGAA	2 cut(s)	329, 732
BstC8I	GCNNGC	5 cut(s)	716, 919, 1170, 1227, 1750
BstDEI	CTNAG	4 cut(s)	257, 843, 1100, 1655
BstDSI	CCRYGG	3 cut(s)	564, 738, 1269
BstEII	GGTNACC	1 cut(s)	559
BstF5I	GGATG	6 cut(s)	33, 105, 455, 810, 985, 1240
BstH2I	RGCGCY	1 cut(s)	791
BstHHI	GCGC	7 cut(s)	307, 683, 790, 917, 1248, 1445, 1502
BstKTI	GATC	8 cut(s)	208, 270, 470, 491, 534, 589, 1059, 1541
BstMAI	GTCTC	7 cut(s)	160, 207, 846, 974, 1058, 1177, 1644
BstMBI	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
BstMCI	CGRYCG	2 cut(s)	253, 589
BstNI	CCWGG	4 cut(s)	56, 359, 653, 1117
BstPAI	GACNNNNGTC	1 cut(s)	1202
BstPI	GGTNACC	1 cut(s)	559
BstSCI	CCNGG	9 cut(s)	54, 357, 463, 510, 555, 651, 932, 1115, 1570
BstSFI	CTRYAG	1 cut(s)	995
BstSLI	GKGCMC	3 cut(s)	530, 1170, 1242
BstV1I	GCAGC	6 cut(s)	180, 195, 412, 1148, 1353, 1654
BstV2I	GAAGAC	2 cut(s)	902, 1202
Bsu36I	CCTNAGG	1 cut(s)	257
BsuRI	GGCC	9 cut(s)	59, 569, 576, 650, 656, 932, 1112, 1240, 1508
BtgI	CCRYGG	3 cut(s)	564, 738, 1269
BtgZI	GCGATG	2 cut(s)	391, 682
BtrI	CACGTC	1 cut(s)	973
BtsCI	GGATG	6 cut(s)	33, 105, 455, 810, 985, 1240
BtsI	GCAGTG	1 cut(s)	1314
BtsIMutI	CAGTG	2 cut(s)	179, 1314
BtuMI	TCGCGA	1 cut(s)	321
BveI	ACCTGC	2 cut(s)	988, 1002
Cac8I	GCNNGC	5 cut(s)	716, 919, 1170, 1227, 1750
CaiI	CAGNNNCTG	1 cut(s)	652
CfoI	GCGC	7 cut(s)	307, 683, 790, 917, 1248, 1445, 1502
Cfr10I	RCCGGY	2 cut(s)	572, 1670
Cfr13I	GGNCC	7 cut(s)	472, 553, 649, 655, 1110, 1238, 1239
Cfr42I	CCGCGG	1 cut(s)	741
CseI	GACGC	6 cut(s)	227, 332, 856, 1274, 1551, 1626
Csp6I	GTAC	4 cut(s)	953, 958, 1174, 1725
CspCI	CAANNNNNGTGG	3 cut(s)	82, 117, 1725
CviAII	CATG	4 cut(s)	703, 1060, 1270, 1718
CviQI	GTAC	4 cut(s)	953, 958, 1174, 1725
DdeI	CTNAG	4 cut(s)	257, 843, 1100, 1655
DpnI	GATC	8 cut(s)	207, 269, 469, 490, 533, 588, 1058, 1540
DpnII	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
DraI	TTTAAA	1 cut(s)	1258
DriI	GACNNNNNGTC	1 cut(s)	1524
EaeI	YGGCCR	4 cut(s)	57, 567, 574, 1506
Eam1104I	CTCTTC	1 cut(s)	1175
Eam1105I	GACNNNNNGTC	1 cut(s)	1524
EarI	CTCTTC	1 cut(s)	1175
EciI	GGCGGA	4 cut(s)	470, 1428, 1568, 1642
Ecl136II	GAGCTC	6 cut(s)	76, 124, 346, 667, 880, 1076
Eco130I	CCWWGG	1 cut(s)	1269
Eco24I	GRGCYC	8 cut(s)	78, 126, 348, 669, 815, 882, 1078, 1242
Eco31I	GGTCTC	1 cut(s)	974
Eco47I	GGWCC	2 cut(s)	472, 553
Eco53kI	GAGCTC	6 cut(s)	76, 124, 346, 667, 880, 1076
Eco72I	CACGTG	2 cut(s)	630, 1202
Eco81I	CCTNAGG	1 cut(s)	257
Eco88I	CYCGRG	3 cut(s)	347, 1187, 1241
Eco91I	GGTNACC	1 cut(s)	559
EcoICRI	GAGCTC	6 cut(s)	76, 124, 346, 667, 880, 1076
EcoO109I	RGGNCCY	2 cut(s)	553, 649
EcoO65I	GGTNACC	1 cut(s)	559
EcoRII	CCWGG	4 cut(s)	54, 357, 651, 1115
EcoT14I	CCWWGG	1 cut(s)	1269
EcoT38I	GRGCYC	8 cut(s)	78, 126, 348, 669, 815, 882, 1078, 1242
ErhI	CCWWGG	1 cut(s)	1269
Esp3I	CGTCTC	3 cut(s)	160, 207, 1644
FaeI	CATG	4 cut(s)	706, 1063, 1273, 1721
FaiI	YATR	9 cut(s)	583, 671, 704, 1061, 1208, 1271, 1438, 1591, 1719
FaqI	GGGAC	5 cut(s)	37, 566, 618, 710, 1624
FatI	CATG	4 cut(s)	702, 1059, 1269, 1717
FauI	CCCGC	5 cut(s)	188, 543, 1241, 1285, 1322
FokI	GGATG	6 cut(s)	40, 92, 442, 817, 972, 1247
FriOI	GRGCYC	8 cut(s)	78, 126, 348, 669, 815, 882, 1078, 1242
FspBI	CTAG	2 cut(s)	791, 1421
GlaI	GCGC	7 cut(s)	306, 682, 789, 916, 1247, 1444, 1501
GsuI	CTGGAG	2 cut(s)	26, 341
HaeII	RGCGCY	1 cut(s)	791
HaeIII	GGCC	9 cut(s)	59, 569, 576, 650, 656, 932, 1112, 1240, 1508
HgaI	GACGC	6 cut(s)	227, 332, 856, 1274, 1551, 1626
HhaI	GCGC	7 cut(s)	307, 683, 790, 917, 1248, 1445, 1502
Hin1I	GRCGYC	5 cut(s)	219, 867, 1266, 1562, 1637
Hin1II	CATG	4 cut(s)	706, 1063, 1273, 1721
Hin6I	GCGC	7 cut(s)	305, 681, 788, 915, 1246, 1443, 1500
HinP1I	GCGC	7 cut(s)	305, 681, 788, 915, 1246, 1443, 1500
HincII	GTYRAC	3 cut(s)	763, 826, 1003
HindII	GTYRAC	3 cut(s)	763, 826, 1003
HindIII	AAGCTT	1 cut(s)	830
HpaI	GTTAAC	1 cut(s)	1003
HphI	GGTGA	7 cut(s)	70, 553, 1414, 1528, 1597, 1627, 1723
Hpy166II	GTNNAC	5 cut(s)	763, 826, 874, 1003, 1168
Hpy188III	TCNNGA	9 cut(s)	43, 259, 320, 349, 370, 590, 618, 883, 1189
Hpy8I	GTNNAC	5 cut(s)	763, 826, 874, 1003, 1168
Hpy99I	CGWCG	7 cut(s)	140, 206, 221, 326, 419, 872, 1018
HpyAV	CCTTC	4 cut(s)	1120, 1319, 1469, 1624
HpyCH4III	ACNGT	8 cut(s)	565, 911, 957, 1348, 1387, 1615, 1711, 1741
HpyCH4IV	ACGT	3 cut(s)	629, 972, 1201
HpyCH4V	TGCA	6 cut(s)	336, 921, 997, 1136, 1168, 1351
HpyF3I	CTNAG	4 cut(s)	257, 843, 1100, 1655
HpySE526I	ACGT	3 cut(s)	629, 972, 1201
Hsp92I	GRCGYC	5 cut(s)	219, 867, 1266, 1562, 1637
Hsp92II	CATG	4 cut(s)	706, 1063, 1273, 1721
HspAI	GCGC	7 cut(s)	305, 681, 788, 915, 1246, 1443, 1500
KflI	GGGWCCC	1 cut(s)	553
Kpn2I	TCCGGA	1 cut(s)	882
KspAI	GTTAAC	1 cut(s)	1003
KspI	CCGCGG	1 cut(s)	741
Kzo9I	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
Lsp1109I	GCAGC	6 cut(s)	180, 195, 412, 1148, 1353, 1654
LweI	GCATC	3 cut(s)	234, 464, 503
MaeI	CTAG	2 cut(s)	791, 1421
MaeII	ACGT	3 cut(s)	629, 972, 1201
MaeIII	GTNAC	4 cut(s)	559, 1009, 1381, 1516
MalI	GATC	8 cut(s)	207, 269, 469, 490, 533, 588, 1058, 1540
MboI	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
MboII	GAAGA	5 cut(s)	17, 907, 1192, 1204, 1207
MlsI	TGGCCA	2 cut(s)	59, 569
MluCI	AATT	8 cut(s)	308, 331, 500, 1285, 1395, 1454, 1556, 1595
MluI	ACGCGT	1 cut(s)	1170
MluNI	TGGCCA	2 cut(s)	59, 569
MlyI	GAGTC	6 cut(s)	59, 414, 732, 895, 896, 1179
MmeI	TCCRAC	5 cut(s)	39, 397, 930, 1371, 1461
Mox20I	TGGCCA	2 cut(s)	59, 569
MroI	TCCGGA	1 cut(s)	882
MscI	TGGCCA	2 cut(s)	59, 569
MseI	TTAA	7 cut(s)	131, 503, 962, 1002, 1023, 1080, 1257
MslI	CAYNNNNRTG	2 cut(s)	701, 1514
Msp20I	TGGCCA	2 cut(s)	59, 569
MspA1I	CMGCKG	2 cut(s)	740, 1139
MspCI	CTTAAG	1 cut(s)	961
MspR9I	CCNGG	9 cut(s)	56, 359, 465, 512, 557, 653, 934, 1117, 1572
Mva1269I	GAATGC	1 cut(s)	1138
MvaI	CCWGG	4 cut(s)	56, 359, 653, 1117
NciI	CCSGG	5 cut(s)	465, 512, 557, 934, 1572
NcoI	CCATGG	1 cut(s)	1269
NdeII	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
NlaIII	CATG	4 cut(s)	706, 1063, 1273, 1721
NlaIV	GGNNCC	8 cut(s)	23, 35, 527, 554, 555, 710, 1111, 1240
NmuCI	GTSAC	3 cut(s)	559, 1009, 1516
NruI	TCGCGA	1 cut(s)	321
NspV	TTCGAA	2 cut(s)	329, 732
OliI	CACNNNNGTG	1 cut(s)	1514
PaeR7I	CTCGAG	1 cut(s)	347
PcsI	WCGNNNNNNNCGW	6 cut(s)	150, 420, 438, 1089, 1487, 1619
PctI	GAATGC	1 cut(s)	1138
PfeI	GAWTC	8 cut(s)	8, 316, 593, 614, 676, 1018, 1651, 1714
PflFI	GACNNNGTC	3 cut(s)	49, 140, 557
PfoI	TCCNGGA	1 cut(s)	357
Ple19I	CGATCG	1 cut(s)	589
PleI	GAGTC	6 cut(s)	58, 414, 731, 894, 896, 1179
PmaCI	CACGTG	2 cut(s)	630, 1202
PmlI	CACGTG	2 cut(s)	630, 1202
PpsI	GAGTC	6 cut(s)	58, 414, 731, 894, 896, 1179
Ppu21I	YACGTR	2 cut(s)	630, 1202
PpuMI	RGGWCCY	1 cut(s)	553
PshAI	GACNNNNGTC	1 cut(s)	1202
Psp124BI	GAGCTC	6 cut(s)	78, 126, 348, 669, 882, 1078
Psp5II	RGGWCCY	1 cut(s)	553
Psp6I	CCWGG	4 cut(s)	54, 357, 651, 1115
PspCI	CACGTG	2 cut(s)	630, 1202
PspEI	GGTNACC	1 cut(s)	559
PspGI	CCWGG	4 cut(s)	54, 357, 651, 1115
PspN4I	GGNNCC	8 cut(s)	23, 35, 527, 554, 555, 710, 1111, 1240
PspOMI	GGGCCC	1 cut(s)	1238
PspPI	GGNCC	7 cut(s)	472, 553, 649, 655, 1110, 1238, 1239
PspPPI	RGGWCCY	1 cut(s)	553
PstI	CTGCAG	1 cut(s)	999
PstNI	CAGNNNCTG	1 cut(s)	652
PsyI	GACNNNGTC	3 cut(s)	49, 140, 557
PvuI	CGATCG	1 cut(s)	589
RruI	TCGCGA	1 cut(s)	321
RsaI	GTAC	4 cut(s)	954, 959, 1175, 1726
RsaNI	GTAC	4 cut(s)	953, 958, 1174, 1725
RseI	CAYNNNNRTG	2 cut(s)	701, 1514
SacI	GAGCTC	6 cut(s)	78, 126, 348, 669, 882, 1078
SacII	CCGCGG	1 cut(s)	741
SaqAI	TTAA	7 cut(s)	131, 503, 962, 1002, 1023, 1080, 1257
Sau3AI	GATC	8 cut(s)	205, 267, 467, 488, 531, 586, 1056, 1538
Sau96I	GGNCC	7 cut(s)	472, 553, 649, 655, 1110, 1238, 1239
SbfI	CCTGCAGG	1 cut(s)	999
SchI	GAGTC	6 cut(s)	59, 414, 732, 895, 896, 1179
ScrFI	CCNGG	9 cut(s)	56, 359, 465, 512, 557, 653, 934, 1117, 1572
SdaI	CCTGCAGG	1 cut(s)	999
SfaNI	GCATC	3 cut(s)	234, 464, 503
SfcI	CTRYAG	1 cut(s)	995
Sfr274I	CTCGAG	1 cut(s)	347
Sfr303I	CCGCGG	1 cut(s)	741
SfuI	TTCGAA	2 cut(s)	329, 732
SgrBI	CCGCGG	1 cut(s)	741
SinI	GGWCC	2 cut(s)	472, 553
SlaI	CTCGAG	1 cut(s)	347
SmiMI	CAYNNNNRTG	2 cut(s)	701, 1514
SmlI	CTYRAG	2 cut(s)	347, 961
SmoI	CTYRAG	2 cut(s)	347, 961
Sse8387I	CCTGCAGG	1 cut(s)	999
Sse9I	AATT	8 cut(s)	308, 331, 500, 1285, 1395, 1454, 1556, 1595
SspMI	CTAG	2 cut(s)	791, 1421
SstI	GAGCTC	6 cut(s)	78, 126, 348, 669, 882, 1078
StyD4I	CCNGG	9 cut(s)	54, 357, 463, 510, 555, 651, 932, 1115, 1570
StyI	CCWWGG	1 cut(s)	1269
TaaI	ACNGT	8 cut(s)	565, 911, 957, 1348, 1387, 1615, 1711, 1741
TaiI	ACGT	3 cut(s)	632, 975, 1204
TaqII	GACCGA	1 cut(s)	62
TasI	AATT	8 cut(s)	308, 331, 500, 1285, 1395, 1454, 1556, 1595
TatI	WGTACW	1 cut(s)	1724
TfiI	GAWTC	8 cut(s)	8, 316, 593, 614, 676, 1018, 1651, 1714
Tru1I	TTAA	7 cut(s)	131, 503, 962, 1002, 1023, 1080, 1257
Tru9I	TTAA	7 cut(s)	131, 503, 962, 1002, 1023, 1080, 1257
TscAI	CASTG	2 cut(s)	179, 1314
TseFI	GTSAC	3 cut(s)	559, 1009, 1516
TseI	GCWGC	6 cut(s)	168, 183, 400, 1136, 1366, 1667
Tsp45I	GTSAC	3 cut(s)	559, 1009, 1516
TspGWI	ACGGA	4 cut(s)	395, 677, 914, 1086
TspRI	CASTG	2 cut(s)	179, 1314
Tth111I	GACNNNGTC	3 cut(s)	49, 140, 557
Vha464I	CTTAAG	1 cut(s)	961
VneI	GTGCAC	1 cut(s)	1166
VpaK11BI	GGWCC	2 cut(s)	472, 553
XapI	RAATTY	1 cut(s)	1285
XhoI	CTCGAG	1 cut(s)	347
XspI	CTAG	2 cut(s)	791, 1421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.