Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0014160

Physical Location & Seq

Genomic Coordinates Forward (+)

26713323 .. 26715383

Gene Structure 2061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1185600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGACAATATCTCCCAAAGCATTCCCGCCGCCAAAACGCCGGCCCACGGCGGGGGCGTTCGTGTCGCCCAAGCTCTCTGACCAGACCCTCCTCCGCTCCCTCCACCATCTCTCCCATGAAATCTCCTCCGTCGAGCCTCTCCACTTCCTCCGCCGCCGCACCTCCCGCTCCATGATCCGCAAGTCAACCCTCCTCTCCATCCTCTTCGAAGACATCATCAGGAGCTCCACCGCCGTCTTCTCCCCCTCCGCCCTCCTCTGCTTCCAGGAGCTCTACATCCTGCTCCAGAGGATCCAGACTCTCATGGAAGATTCCTCCAACGGGAGCCGGATGTGGCTGCTCCTCCAGACCGAGTCCCTCGCCAACAGCTTCCACGAGCTCACCCTCGACCTCTCTACCTTGCTTGACATCCTCCCCGTCAAGGAGCTCCGATTAAACGACGATGTCCGGGAGGTCGTCTCCCTCCTCCGCAGCCAGTGCTCCCGCAGCACCACAAAATCGCTCGTCGATCCGAGAGACGACGCCCTCCGCCGCGAGGTTTGCTCCATGCTCGACCGCCTCAGGAGCGAGATCGTCCCTGACCACTCCAAGCTCTCTGAAATCTTCGCGCAATTAGGGATTCGCGACGCTTCGAATTGCAGAGAGGAGCTCGAGAGCCTCCAGGACGAGTTCCAGAACCAGACGGAGGAGAAACCCAAGTCGCAGCTCATCTCCCTCGTCGGACTCGTTCGCTACGGCAAGTGCGTGTTATTTGGAGCATCCACACCCGGATCGGACCTAATCCGCCGTAAATCGGCATCGGAATTAAGTTTCCCGGTAGATTTTCGGTGTCCGATCAGTTTGGAGCTAATGCGGGACCCGGTCACCGTGGCCACCGGCCAGACATACGATCGTGATTCCATCAAGCTGTGGATCGAATCAGGACACAACACGTGTCCCAAGACAGGTCAGGCCCTGGCCCACATGGAGATCATACCGAATCGCGCGTTGAAAAATCTCATCGCCATGTGGTGCCGCGAGCAGAGAGTCCCTTTCGAAACCTCGGAGAATAGTAAGGGGCTAGTCAACGCCGTAAAAAAGAACAAGGCGGCGCTAGAAGCTACGCGGATGACGGGCTCGTTTTTGGTCAACAAGCTTTCGGTGTCTCAGTCGATGGAGGCGGCGAACGGCGTCGTTTACGAGCTCCGGAGTCTGGCGAAGACGGACTCCGACTGTCGCGCCTGCATTGCGGAGGCCGGGGCTATTCCTTTGCTTGTACGGTACTTAAGCTCCGACGTGGGAGAGACCCATCCGAACCTGCAGGTTAACGCCGTGACGACGATTCTTAACCTTTCTATCTTGGAAGCGAACAAAACGCGGATCATGGAGACTGACGGAGCTCTTAACGGCGTTATCGAGGTGCTGAGGTCGGGGGCCACCTGGGAGGCGAAGGGGAATGCGGCGGCTACGATATTCAGTTTATCAGGTGTGCAGGCGTACAGGAAGAGACTCGGGAAGAAGACACGTGTCATAAAGGGGTTAGTGGAGTTGGCGAGGGATGGGCCCGAGCGCGGGTGTTTTAAAAAGGACGCCATGGTGGCGATACTGAATTTGGCGGGAGAGAGGGAGGCAGTGGCGAGATTGGTGGAAGGCGGGGTGGTGGAAATGACCGTGCAAGTGATGGAGGGGCTGCCGGAGGAGGCGGTTACTGTGTTGGAAATTGTGGTGAGAAGCGGCGGATTACTGGCCATTGCGGCGGCGCATCGCGCGATTAGAAAATTGGGGAGTGTGTTGAGAGAAGGGTCGGAGAGGGCGAGGGAGAGCGCGGCGGCCACGCTGGTGACAATCTGTCGAAAAGGGGGATCGGAGATGGTGGCGGAATTGGCGTCAATGCCAGGGATTGAGAGGGTGATATGGGAATTGATGGGGGTGGGGACGGTGAGGGCGAGGCGGAAGGCGGCGTCTCTATTGAGGATTCTGAGGAGATGGGCTGCCGGTTTGGATAGTGAGGTAGCGGAGGGGGGATATTCAACGGTGAATCATGTGAGTACATCGGCAACAACAACAGTAGTGGCAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

687

Amino Acids

75.31

Weight (kDa)

8.97

Isoelectric Point (pI)

46.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	52 - 163	1.2e-10	PUB10-like, N-terminal domain
U-box	PF04564	272 - 342	2.4e-19	U-box domain
ARM_ARMC5	PF24768	373 - 540	4.5e-06	ARMC5
ARM_PUB	PF25598	376 - 655	9.9e-18	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014160)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01830
fragaria_vesca	FvH4_5g29050
malus_domestica	MD04G1172400.v1.1 MD12G1185600.v1.1
prunus_persica	Prupe.6G290800_v2.0.a1
pyrus_communis	pycom04g15230 pycom12g17420
rosa_chinensis	RchiOBHm_Chr3g0460861
rosa_laevigata	RLG00000024973
rosa_multiflora	Rmu_sc0009011.1_g000007
rosa_roxburghii	Rroxscaffold_6G00419150
rosa_rugosa	Rorug03G0047000
rosa_samantha	Rh3AG104100 Rh3BG107800 Rh3CG109600 Rh3DG109600
rosa_wichuraiana	Rw3G008860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1291, 1305
AccB1I	GGYRCC	1 cut(s)	1011
AccBSI	CCGCTC	2 cut(s)	96, 168
AccIII	TCCGGA	1 cut(s)	1185
AclWI	GGATC	8 cut(s)	169, 286, 299, 503, 778, 920, 1367, 1849
AcoI	YGGCCR	4 cut(s)	870, 877, 1725, 1809
AcsI	RAATTY	1 cut(s)	1588
AcvI	CACGTG	2 cut(s)	933, 1505
AcyI	GRCGYC	5 cut(s)	522, 1170, 1569, 1865, 1940
AfaI	GTAC	4 cut(s)	1257, 1262, 1478, 2029
AflII	CTTAAG	1 cut(s)	1264
AflIII	ACRYGT	4 cut(s)	930, 932, 1502, 1504
AgsI	TTSAA	2 cut(s)	991, 2010
AhdI	GACNNNNNGTC	1 cut(s)	1827
AjiI	CACGTC	1 cut(s)	1276
AjnI	CCWGG	5 cut(s)	264, 660, 954, 1418, 1873
AleI	CACNNNNGTG	1 cut(s)	1817
Alw21I	GWGCWC	8 cut(s)	227, 273, 381, 429, 482, 651, 1185, 1381
Alw26I	GTCTC	7 cut(s)	463, 510, 1149, 1277, 1361, 1480, 1947
AlwI	GGATC	8 cut(s)	169, 286, 299, 503, 778, 920, 1367, 1849
AlwNI	CAGNNNCTG	1 cut(s)	955
Ama87I	CYCGRG	3 cut(s)	650, 1490, 1544
Aor13HI	TCCGGA	1 cut(s)	1185
ApaI	GGGCCC	1 cut(s)	1545
ApeKI	GCWGC	6 cut(s)	337, 471, 486, 703, 1669, 1970
ApoI	RAATTY	1 cut(s)	1588
AspLEI	GCGC	8 cut(s)	610, 986, 1093, 1220, 1551, 1741, 1748, 1805
AspS9I	GGNCC	8 cut(s)	42, 775, 856, 952, 958, 1413, 1541, 1542
AsuC2I	CCSGG	5 cut(s)	449, 768, 815, 860, 1237
AsuHPI	GGTGA	7 cut(s)	373, 856, 1717, 1831, 1900, 1930, 2026
AsuII	TTCGAA	3 cut(s)	207, 632, 1035
AvaI	CYCGRG	3 cut(s)	650, 1490, 1544
AvaII	GGWCC	2 cut(s)	775, 856
AxyI	CCTNAGG	1 cut(s)	560
BaeGI	GKGCMC	1 cut(s)	1545
BalI	TGGCCA	2 cut(s)	872, 1727
BamHI	GGATCC	1 cut(s)	291
BanI	GGYRCC	1 cut(s)	1011
BanII	GRGCYC	9 cut(s)	227, 273, 381, 429, 651, 1118, 1185, 1381, 1545
BauI	CACGAG	1 cut(s)	374
BbrPI	CACGTG	2 cut(s)	933, 1505
BbsI	GAAGAC	4 cut(s)	216, 229, 1205, 1505
Bbv12I	GWGCWC	8 cut(s)	227, 273, 381, 429, 482, 651, 1185, 1381
BbvCI	CCTCAGC	1 cut(s)	1403
BbvI	GCAGC	6 cut(s)	324, 483, 498, 715, 1656, 1957
BceAI	ACGGC	8 cut(s)	63, 218, 751, 771, 1055, 1183, 1295, 1402
BciT130I	CCWGG	5 cut(s)	266, 662, 956, 1420, 1875
BcnI	CCSGG	5 cut(s)	449, 768, 815, 860, 1237
BcoDI	GTCTC	7 cut(s)	463, 510, 1149, 1277, 1361, 1480, 1947
BfaI	CTAG	2 cut(s)	1061, 1094
BfmI	CTRYAG	1 cut(s)	1298
BfoI	RGCGCY	1 cut(s)	1094
BfrI	CTTAAG	1 cut(s)	1264
BfuAI	ACCTGC	2 cut(s)	1291, 1305
BglI	GCCNNNNNGGC	2 cut(s)	1577, 1733
Bme18I	GGWCC	2 cut(s)	775, 856
BmeRI	GACNNNNNGTC	1 cut(s)	1827
BmeT110I	CYCGRG	3 cut(s)	650, 1490, 1544
BmgBI	CACGTC	1 cut(s)	1276
BmgT120I	GGNCC	8 cut(s)	42, 775, 856, 952, 958, 1413, 1541, 1542
BmiI	GGNNCC	7 cut(s)	293, 326, 857, 858, 1013, 1414, 1543
BmsI	GCATC	3 cut(s)	767, 806, 1750
BoxI	GACNNNNGTC	1 cut(s)	1505
BpiI	GAAGAC	4 cut(s)	216, 229, 1205, 1505
BplI	GAGNNNNNCTC	6 cut(s)	443, 475, 527, 559, 633, 665
BpmI	CTGGAG	3 cut(s)	269, 329, 644
Bpu10I	CCTNAGC	1 cut(s)	1403
Bpu14I	TTCGAA	3 cut(s)	207, 632, 1035
BpuMI	CCSGG	5 cut(s)	449, 768, 815, 860, 1237
BsaAI	YACGTR	2 cut(s)	933, 1505
BsaHI	GRCGYC	5 cut(s)	522, 1170, 1569, 1865, 1940
BsaI	GGTCTC	1 cut(s)	1277
BsaJI	CCNNGG	8 cut(s)	45, 867, 954, 1041, 1236, 1419, 1572, 1874
BsaWI	WCCGGW	1 cut(s)	1185
BsaXI	ACNNNNNCTCC	5 cut(s)	25, 95, 125, 1253, 1283
Bse118I	RCCGGY	3 cut(s)	39, 875, 1973
Bse1I	ACTGG	2 cut(s)	475, 1728
Bse21I	CCTNAGG	1 cut(s)	560
Bse3DI	GCAATG	2 cut(s)	1224, 1728
BseAI	TCCGGA	1 cut(s)	1185
BseBI	CCWGG	5 cut(s)	266, 662, 956, 1420, 1875
BseDI	CCNNGG	8 cut(s)	45, 867, 954, 1041, 1236, 1419, 1572, 1874
BseGI	GGATG	8 cut(s)	198, 276, 336, 408, 758, 1113, 1288, 1543
BseMI	GCAATG	2 cut(s)	1224, 1728
BseMII	CTCAG	4 cut(s)	574, 1160, 1394, 1949
BseNI	ACTGG	2 cut(s)	475, 1728
BseSI	GKGCMC	1 cut(s)	1545
BseXI	GCAGC	6 cut(s)	324, 483, 498, 715, 1656, 1957
BsgI	GTGCAG	1 cut(s)	1490
Bsh1285I	CGRYCG	2 cut(s)	556, 892
BshNI	GGYRCC	1 cut(s)	1011
BsiEI	CGRYCG	2 cut(s)	556, 892
BsiHKAI	GWGCWC	8 cut(s)	227, 273, 381, 429, 482, 651, 1185, 1381
BsiHKCI	CYCGRG	3 cut(s)	650, 1490, 1544
BslFI	GGGAC	6 cut(s)	340, 560, 869, 921, 1013, 1927
BsmAI	GTCTC	7 cut(s)	463, 510, 1149, 1277, 1361, 1480, 1947
BsmBI	CGTCTC	3 cut(s)	463, 510, 1947
BsmFI	GGGAC	6 cut(s)	340, 560, 869, 921, 1013, 1927
BsmI	GAATGC	2 cut(s)	20, 1441
Bso31I	GGTCTC	1 cut(s)	1277
BsoBI	CYCGRG	3 cut(s)	650, 1490, 1544
Bsp119I	TTCGAA	3 cut(s)	207, 632, 1035
Bsp120I	GGGCCC	1 cut(s)	1541
Bsp13I	TCCGGA	1 cut(s)	1185
Bsp19I	CCATGG	1 cut(s)	1572
Bsp68I	TCGCGA	1 cut(s)	624
BspCNI	CTCAG	4 cut(s)	573, 1159, 1395, 1950
BspEI	TCCGGA	1 cut(s)	1185
BspLI	GGNNCC	7 cut(s)	293, 326, 857, 858, 1013, 1414, 1543
BspMAI	CTGCAG	1 cut(s)	1302
BspMI	ACCTGC	2 cut(s)	1291, 1305
BspPI	GGATC	8 cut(s)	169, 286, 299, 503, 778, 920, 1367, 1849
BspT104I	TTCGAA	3 cut(s)	207, 632, 1035
BspT107I	GGYRCC	1 cut(s)	1011
BspTI	CTTAAG	1 cut(s)	1264
BspTNI	GGTCTC	1 cut(s)	1277
BsrBI	CCGCTC	2 cut(s)	96, 168
BsrDI	GCAATG	2 cut(s)	1224, 1728
BsrFI	RCCGGY	3 cut(s)	39, 875, 1973
BsrI	ACTGG	2 cut(s)	475, 1728
BssAI	RCCGGY	3 cut(s)	39, 875, 1973
BssECI	CCNNGG	8 cut(s)	45, 867, 954, 1041, 1236, 1419, 1572, 1874
BssNI	GRCGYC	5 cut(s)	522, 1170, 1569, 1865, 1940
BssSI	CACGAG	1 cut(s)	374
BssT1I	CCWWGG	1 cut(s)	1572
Bst2BI	CACGAG	1 cut(s)	374
Bst2UI	CCWGG	5 cut(s)	266, 662, 956, 1420, 1875
Bst4CI	ACNGT	8 cut(s)	868, 1214, 1260, 1651, 1690, 1918, 2014, 2047
Bst6I	CTCTTC	2 cut(s)	209, 1478
BstACI	GRCGYC	5 cut(s)	522, 1170, 1569, 1865, 1940
BstAFI	CTTAAG	1 cut(s)	1264
BstAPI	GCANNNNNTGC	1 cut(s)	477
BstBAI	YACGTR	2 cut(s)	933, 1505
BstBI	TTCGAA	3 cut(s)	207, 632, 1035
BstC8I	GCNNGC	5 cut(s)	41, 1019, 1222, 1473, 2056
BstDEI	CTNAG	4 cut(s)	560, 1146, 1403, 1958
BstDSI	CCRYGG	3 cut(s)	45, 867, 1572
BstEII	GGTNACC	1 cut(s)	862
BstF5I	GGATG	8 cut(s)	198, 276, 336, 408, 758, 1113, 1288, 1543
BstH2I	RGCGCY	1 cut(s)	1094
BstHHI	GCGC	8 cut(s)	610, 986, 1093, 1220, 1551, 1741, 1748, 1805
BstMAI	GTCTC	7 cut(s)	463, 510, 1149, 1277, 1361, 1480, 1947
BstMCI	CGRYCG	2 cut(s)	556, 892
BstNI	CCWGG	5 cut(s)	266, 662, 956, 1420, 1875
BstPAI	GACNNNNGTC	1 cut(s)	1505
BstPI	GGTNACC	1 cut(s)	862
BstSFI	CTRYAG	1 cut(s)	1298
BstSLI	GKGCMC	1 cut(s)	1545
BstV1I	GCAGC	6 cut(s)	324, 483, 498, 715, 1656, 1957
BstV2I	GAAGAC	4 cut(s)	216, 229, 1205, 1505
BstX2I	RGATCY	1 cut(s)	291
BstYI	RGATCY	1 cut(s)	291
Bsu36I	CCTNAGG	1 cut(s)	560
BtgI	CCRYGG	3 cut(s)	45, 867, 1572
BtgZI	GCGATG	2 cut(s)	985, 1727
BtrI	CACGTC	1 cut(s)	1276
BtsCI	GGATG	8 cut(s)	198, 276, 336, 408, 758, 1113, 1288, 1543
BtsI	GCAGTG	1 cut(s)	1617
BtsIMutI	CAGTG	2 cut(s)	482, 1617
BtuMI	TCGCGA	1 cut(s)	624
BveI	ACCTGC	2 cut(s)	1291, 1305
Cac8I	GCNNGC	5 cut(s)	41, 1019, 1222, 1473, 2056
CaiI	CAGNNNCTG	1 cut(s)	955
CfoI	GCGC	8 cut(s)	610, 986, 1093, 1220, 1551, 1741, 1748, 1805
Cfr10I	RCCGGY	3 cut(s)	39, 875, 1973
Cfr13I	GGNCC	8 cut(s)	42, 775, 856, 952, 958, 1413, 1541, 1542
CseI	GACGC	6 cut(s)	530, 635, 1159, 1577, 1854, 1929
Csp6I	GTAC	4 cut(s)	1256, 1261, 1477, 2028
CspCI	CAANNNNNGTGG	1 cut(s)	2031
CviAII	CATG	9 cut(s)	116, 172, 304, 547, 964, 1006, 1363, 1573, 2021
CviQI	GTAC	4 cut(s)	1256, 1261, 1477, 2028
DdeI	CTNAG	4 cut(s)	560, 1146, 1403, 1958
DraI	TTTAAA	1 cut(s)	1561
DriI	GACNNNNNGTC	1 cut(s)	1827
EaeI	YGGCCR	4 cut(s)	870, 877, 1725, 1809
Eam1104I	CTCTTC	2 cut(s)	209, 1478
Eam1105I	GACNNNNNGTC	1 cut(s)	1827
EarI	CTCTTC	2 cut(s)	209, 1478
EciI	GGCGGA	7 cut(s)	140, 238, 518, 773, 1731, 1871, 1945
Ecl136II	GAGCTC	7 cut(s)	225, 271, 379, 427, 649, 1183, 1379
Eco130I	CCWWGG	1 cut(s)	1572
Eco24I	GRGCYC	9 cut(s)	227, 273, 381, 429, 651, 1118, 1185, 1381, 1545
Eco31I	GGTCTC	1 cut(s)	1277
Eco47I	GGWCC	2 cut(s)	775, 856
Eco53kI	GAGCTC	7 cut(s)	225, 271, 379, 427, 649, 1183, 1379
Eco72I	CACGTG	2 cut(s)	933, 1505
Eco81I	CCTNAGG	1 cut(s)	560
Eco88I	CYCGRG	3 cut(s)	650, 1490, 1544
Eco91I	GGTNACC	1 cut(s)	862
EcoICRI	GAGCTC	7 cut(s)	225, 271, 379, 427, 649, 1183, 1379
EcoO109I	RGGNCCY	2 cut(s)	856, 952
EcoO65I	GGTNACC	1 cut(s)	862
EcoRII	CCWGG	5 cut(s)	264, 660, 954, 1418, 1873
EcoT14I	CCWWGG	1 cut(s)	1572
EcoT38I	GRGCYC	9 cut(s)	227, 273, 381, 429, 651, 1118, 1185, 1381, 1545
ErhI	CCWWGG	1 cut(s)	1572
Esp3I	CGTCTC	3 cut(s)	463, 510, 1947
FaeI	CATG	9 cut(s)	119, 175, 307, 550, 967, 1009, 1366, 1576, 2024
FaqI	GGGAC	6 cut(s)	340, 560, 869, 921, 1013, 1927
FatI	CATG	9 cut(s)	115, 171, 303, 546, 963, 1005, 1362, 1572, 2020
FauI	CCCGC	8 cut(s)	33, 43, 173, 491, 846, 1544, 1588, 1625
FokI	GGATG	8 cut(s)	185, 263, 343, 395, 745, 1120, 1275, 1550
FriOI	GRGCYC	9 cut(s)	227, 273, 381, 429, 651, 1118, 1185, 1381, 1545
FspBI	CTAG	2 cut(s)	1061, 1094
GlaI	GCGC	8 cut(s)	609, 985, 1092, 1219, 1550, 1740, 1747, 1804
GsuI	CTGGAG	3 cut(s)	269, 329, 644
HaeII	RGCGCY	1 cut(s)	1094
HgaI	GACGC	6 cut(s)	530, 635, 1159, 1577, 1854, 1929
HhaI	GCGC	8 cut(s)	610, 986, 1093, 1220, 1551, 1741, 1748, 1805
Hin1I	GRCGYC	5 cut(s)	522, 1170, 1569, 1865, 1940
Hin1II	CATG	9 cut(s)	119, 175, 307, 550, 967, 1009, 1366, 1576, 2024
Hin6I	GCGC	8 cut(s)	608, 984, 1091, 1218, 1549, 1739, 1746, 1803
HinP1I	GCGC	8 cut(s)	608, 984, 1091, 1218, 1549, 1739, 1746, 1803
HincII	GTYRAC	4 cut(s)	186, 1066, 1129, 1306
HindII	GTYRAC	4 cut(s)	186, 1066, 1129, 1306
HindIII	AAGCTT	1 cut(s)	1133
HpaI	GTTAAC	1 cut(s)	1306
HphI	GGTGA	7 cut(s)	373, 856, 1717, 1831, 1900, 1930, 2026
Hpy166II	GTNNAC	5 cut(s)	186, 1066, 1129, 1177, 1306
Hpy8I	GTNNAC	5 cut(s)	186, 1066, 1129, 1177, 1306
Hpy99I	CGWCG	9 cut(s)	134, 443, 509, 524, 629, 722, 1175, 1277, 1321
HpyAV	CCTTC	4 cut(s)	1423, 1622, 1772, 1927
HpyCH4III	ACNGT	8 cut(s)	868, 1214, 1260, 1651, 1690, 1918, 2014, 2047
HpyCH4IV	ACGT	3 cut(s)	932, 1275, 1504
HpyCH4V	TGCA	5 cut(s)	639, 1224, 1300, 1471, 1654
HpyF3I	CTNAG	4 cut(s)	560, 1146, 1403, 1958
HpySE526I	ACGT	3 cut(s)	932, 1275, 1504
Hsp92I	GRCGYC	5 cut(s)	522, 1170, 1569, 1865, 1940
Hsp92II	CATG	9 cut(s)	119, 175, 307, 550, 967, 1009, 1366, 1576, 2024
HspAI	GCGC	8 cut(s)	608, 984, 1091, 1218, 1549, 1739, 1746, 1803
KflI	GGGWCCC	1 cut(s)	856
Kpn2I	TCCGGA	1 cut(s)	1185
KroI	GCCGGC	1 cut(s)	39
KroNI	GCCGGC	1 cut(s)	41
KspAI	GTTAAC	1 cut(s)	1306
Lsp1109I	GCAGC	6 cut(s)	324, 483, 498, 715, 1656, 1957
LweI	GCATC	3 cut(s)	767, 806, 1750
MaeI	CTAG	2 cut(s)	1061, 1094
MaeII	ACGT	3 cut(s)	932, 1275, 1504
MaeIII	GTNAC	4 cut(s)	862, 1312, 1684, 1819
MbiI	CCGCTC	2 cut(s)	96, 168
MboII	GAAGA	9 cut(s)	196, 221, 229, 320, 595, 1210, 1495, 1507, 1510
MflI	RGATCY	1 cut(s)	291
MlsI	TGGCCA	2 cut(s)	872, 1727
MluCI	AATT	8 cut(s)	611, 634, 803, 1588, 1698, 1757, 1859, 1898
MluNI	TGGCCA	2 cut(s)	872, 1727
MlyI	GAGTC	7 cut(s)	292, 362, 717, 1035, 1198, 1199, 1482
MmeI	TCCRAC	6 cut(s)	342, 700, 1233, 1296, 1674, 1764
Mox20I	TGGCCA	2 cut(s)	872, 1727
MroI	TCCGGA	1 cut(s)	1185
MroNI	GCCGGC	1 cut(s)	39
MscI	TGGCCA	2 cut(s)	872, 1727
MseI	TTAA	7 cut(s)	434, 806, 1265, 1305, 1326, 1383, 1560
MslI	CAYNNNNRTG	2 cut(s)	1004, 1817
Msp20I	TGGCCA	2 cut(s)	872, 1727
MspCI	CTTAAG	1 cut(s)	1264
Mva1269I	GAATGC	2 cut(s)	20, 1441
MvaI	CCWGG	5 cut(s)	266, 662, 956, 1420, 1875
NaeI	GCCGGC	1 cut(s)	41
NciI	CCSGG	5 cut(s)	449, 768, 815, 860, 1237
NcoI	CCATGG	1 cut(s)	1572
NgoMIV	GCCGGC	1 cut(s)	39
NlaIII	CATG	9 cut(s)	119, 175, 307, 550, 967, 1009, 1366, 1576, 2024
NlaIV	GGNNCC	7 cut(s)	293, 326, 857, 858, 1013, 1414, 1543
NmuCI	GTSAC	3 cut(s)	862, 1312, 1819
NruI	TCGCGA	1 cut(s)	624
NspV	TTCGAA	3 cut(s)	207, 632, 1035
OliI	CACNNNNGTG	1 cut(s)	1817
PaeR7I	CTCGAG	1 cut(s)	650
PcsI	WCGNNNNNNNCGW	6 cut(s)	53, 723, 741, 1392, 1790, 1922
PctI	GAATGC	2 cut(s)	20, 1441
PdiI	GCCGGC	1 cut(s)	41
PfeI	GAWTC	8 cut(s)	311, 619, 896, 917, 979, 1321, 1954, 2017
PflFI	GACNNNGTC	3 cut(s)	352, 443, 860
PfoI	TCCNGGA	3 cut(s)	264, 447, 660
Ple19I	CGATCG	1 cut(s)	892
PleI	GAGTC	7 cut(s)	292, 361, 717, 1034, 1197, 1199, 1482
PmaCI	CACGTG	2 cut(s)	933, 1505
PmlI	CACGTG	2 cut(s)	933, 1505
PpsI	GAGTC	7 cut(s)	292, 361, 717, 1034, 1197, 1199, 1482
Ppu21I	YACGTR	2 cut(s)	933, 1505
PpuMI	RGGWCCY	1 cut(s)	856
PshAI	GACNNNNGTC	1 cut(s)	1505
Psp124BI	GAGCTC	7 cut(s)	227, 273, 381, 429, 651, 1185, 1381
Psp5II	RGGWCCY	1 cut(s)	856
Psp6I	CCWGG	5 cut(s)	264, 660, 954, 1418, 1873
PspCI	CACGTG	2 cut(s)	933, 1505
PspEI	GGTNACC	1 cut(s)	862
PspGI	CCWGG	5 cut(s)	264, 660, 954, 1418, 1873
PspN4I	GGNNCC	7 cut(s)	293, 326, 857, 858, 1013, 1414, 1543
PspOMI	GGGCCC	1 cut(s)	1541
PspPI	GGNCC	8 cut(s)	42, 775, 856, 952, 958, 1413, 1541, 1542
PspPPI	RGGWCCY	1 cut(s)	856
PstI	CTGCAG	1 cut(s)	1302
PstNI	CAGNNNCTG	1 cut(s)	955
PsuI	RGATCY	1 cut(s)	291
PsyI	GACNNNGTC	3 cut(s)	352, 443, 860
PvuI	CGATCG	1 cut(s)	892
RruI	TCGCGA	1 cut(s)	624
RsaI	GTAC	4 cut(s)	1257, 1262, 1478, 2029
RsaNI	GTAC	4 cut(s)	1256, 1261, 1477, 2028
RseI	CAYNNNNRTG	2 cut(s)	1004, 1817
SacI	GAGCTC	7 cut(s)	227, 273, 381, 429, 651, 1185, 1381
SaqAI	TTAA	7 cut(s)	434, 806, 1265, 1305, 1326, 1383, 1560
Sau96I	GGNCC	8 cut(s)	42, 775, 856, 952, 958, 1413, 1541, 1542
SbfI	CCTGCAGG	1 cut(s)	1302
SchI	GAGTC	7 cut(s)	292, 362, 717, 1035, 1198, 1199, 1482
SdaI	CCTGCAGG	1 cut(s)	1302
SfaNI	GCATC	3 cut(s)	767, 806, 1750
SfcI	CTRYAG	1 cut(s)	1298
Sfr274I	CTCGAG	1 cut(s)	650
SfuI	TTCGAA	3 cut(s)	207, 632, 1035
SinI	GGWCC	2 cut(s)	775, 856
SlaI	CTCGAG	1 cut(s)	650
SmiMI	CAYNNNNRTG	2 cut(s)	1004, 1817
SmlI	CTYRAG	2 cut(s)	650, 1264
SmoI	CTYRAG	2 cut(s)	650, 1264
Sse8387I	CCTGCAGG	1 cut(s)	1302
Sse9I	AATT	8 cut(s)	611, 634, 803, 1588, 1698, 1757, 1859, 1898
SspMI	CTAG	2 cut(s)	1061, 1094
SstI	GAGCTC	7 cut(s)	227, 273, 381, 429, 651, 1185, 1381
StyI	CCWWGG	1 cut(s)	1572
TaaI	ACNGT	8 cut(s)	868, 1214, 1260, 1651, 1690, 1918, 2014, 2047
TaiI	ACGT	3 cut(s)	935, 1278, 1507
TaqII	GACCGA	1 cut(s)	365
TasI	AATT	8 cut(s)	611, 634, 803, 1588, 1698, 1757, 1859, 1898
TatI	WGTACW	1 cut(s)	2027
TfiI	GAWTC	8 cut(s)	311, 619, 896, 917, 979, 1321, 1954, 2017
Tru1I	TTAA	7 cut(s)	434, 806, 1265, 1305, 1326, 1383, 1560
Tru9I	TTAA	7 cut(s)	434, 806, 1265, 1305, 1326, 1383, 1560
TscAI	CASTG	2 cut(s)	482, 1617
TseFI	GTSAC	3 cut(s)	862, 1312, 1819
TseI	GCWGC	6 cut(s)	337, 471, 486, 703, 1669, 1970
Tsp45I	GTSAC	3 cut(s)	862, 1312, 1819
TspDTI	ATGAA	1 cut(s)	132
TspGWI	ACGGA	4 cut(s)	118, 698, 1217, 1389
TspRI	CASTG	2 cut(s)	482, 1617
Tth111I	GACNNNGTC	3 cut(s)	352, 443, 860
Vha464I	CTTAAG	1 cut(s)	1264
VpaK11BI	GGWCC	2 cut(s)	775, 856
XapI	RAATTY	1 cut(s)	1588
XhoI	CTCGAG	1 cut(s)	650
XspI	CTAG	2 cut(s)	1061, 1094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.