RchiOBHm_Chr3g0460861

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0014160
Physical Location & Seq
Reverse (-)
8913311 .. 8915556
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42735

Sequence Viewer

Length: 2040 bp
ATGGCGGTTCCTCCACAAGCATTCCCGCCCAGAAAGCGGCGGCCATCTGCAGGAGCCTTCATGTCTCCCAAATTCTCCGACCAGAGCCTCCTCCGCTCTCTCCTCCACCTCTGCCAGGAAATATCCTCCCTCGAATCCCGCAGCTTCCTCCTCAGACGAACCACCCTATCCATGACTCGAAAAGCCAAGCTTCTTGCCTTCCTCTTCGAAGACCTAGTTCGATCATCAATCACCTACCTCTCTCCCTCCGCTCTCTTATGTTTCCAAGAAATGTACATACTGCTGCAGAGAATCCAAACCCTCATCGAAGACTCTTCTCACGGCAGCAAGATGTGGCTTCTCATCCAAAACGAGTCCCTCGCCAACAGCTTCCACGAGCTGACTCTTGATCTATCCACTTTGCTCGATATTCTCCCCGTCAAGGAGCTTAACCTAAACGAAGACGTCGAGGAAGTCGTCGCCTTGCTCCGCGCACAGTGCGCACGAAGCAAGGACGCTTTTGTTGATCCCAAGGATGATAATCTCCGCCTCGAAGTCTGCTCCATGCTCGATCGCCTCAAAAAGGAAATCATTCCAGACCACTCCAAGCTCTCCGAGATTTTTGTCCAGTTAGGAATTCTAGACTCTTCCAAATGTAAAGAAGAGCTAGAGTCTCTCCAAGACGAGTTTCAGAACCAAGCCGACGACAAGTCCAAATCCCAATTGGTTTCCCTCATCGGCCTCGTTCGCTACGCCAAATGTGTCCTCTTCGGCGCTTCAACTCCAAGTTCCGATCTCACTCGCCTGAAATCTTCCTCGGAGCTAAACCTCCCGGCGGACTTCCGGTGTCCGATCAGCTTAGAGCTTATGCGGGACCCGGTCGCCGTGGCCACCGGTCAGACATACGATCGTGAGTCCATCAAGCTGTGGATCGAATCAGGACACAATACGTGTCCCAAGACAGGTCAGACGCTGGCCCACACGAACTTGATACCCAACCGCGCTTTGAAAAATCTTATCGCCATGTGGTGCCGCGAGCAGCGAATCCCTTTCGAGCATGCCGAGAATAAGGATAAAGTCAGCGCCGTCAAGAAGAACAAGGCGGCGTTAGAAGCCACCAGGATGACCGCGTCGTTTTTGGTCAACAAACTCTCCCTCGCCCAGTCAATCGAGGCTGCCAACGGCGTCGTTTACGAGCTTCGTAGCCTGGCCAAGACTGATTCGGACTGCCGAGCCTGCATTGCCGAAGCTGGAGCGATTCCTTTGCTCGTACGTCATTTAGGCTCGGACGCCGGTCGGGACCACCCGAACCTACAGGTGAACGCCGTCACGACGATCCTCAACCTCTCAATACTCGAAGCCAACAAAGCAAGGATCATGGACACTGACGGAGCCTTAAACGGCGTCATTGAGGTGCTTCGTTCGGGTGCCACGTGGGAGGCTAAAGGGAACGCGGCGGCTACGATATTCAGCTTATCAGGTGTGCAAGCGTACAGGAAGAAACTAGGGAGGAAGGCACGTGTCATAAAGGCATTGGTGGACTTGGCCAGGGAGGGACCCGGGTTTTCCAAAAGGGACGCCATGGTGGCGATATTGAATTTGGCGGGAGAAAGAGAGGCGGTGGCGAAGTTGGTGGAATGCGGGGTGGTGGAGATGACGATGGAAGTAATGGAGGGGCTACCGGAAGAGGCGGTGACGGTGTTGGAAGTGGTGGTGAGAGGAGGAGGGATGGGGGCAATTGCGGCGGCGTATGGGGCTATCAGGCATTTGGGAGTGGTGTTGAGAGAAGGGTCGGATAGGGCGAGGGAGAGCGCGGCGGCGACACTTGTGACAATTTGTAGAAAAGGGGGGTCGGAAATGGTGGCGGAGTTGGTGTCGGTTTCGGGGATAGAGAGGGTAATATGGGAATTGATGGGGACGGGGACGGTGAGGGCGAGGAGGAAAGCGGCGTCGTTGTTGAGGATCTTGAGGAGATGGGCGGCCGGATTGGAGGGTGATCAAGTAGTGGAAGGGTATTCGACTATTGTGAGTTCATCAACGACAGCAATGGTGTCTGGCTAA

Protein Analysis

679

Amino Acids

74.29

Weight (kDa)

8.31

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB10_N PF25368 52 - 164 7.2e-11 PUB10-like, N-terminal domain
U-box PF04564 271 - 341 1.8e-20 U-box domain
ARM_ARMC5 PF24768 368 - 549 4.8e-06 ARMC5
ARM_PUB PF25598 377 - 651 2.5e-14 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01830
fragaria_vesca FvH4_5g29050
malus_domestica MD04G1172400.v1.1 MD12G1185600.v1.1
prunus_persica Prupe.6G290800_v2.0.a1
pyrus_communis pycom04g15230 pycom12g17420
rosa_chinensis RchiOBHm_Chr3g0460861
rosa_laevigata RLG00000024973
rosa_multiflora Rmu_sc0009011.1_g000007
rosa_roxburghii Rroxscaffold_6G00419150
rosa_rugosa Rorug03G0047000
rosa_samantha Rh3AG104100 Rh3BG107800 Rh3CG109600 Rh3DG109600
rosa_wichuraiana Rw3G008860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 447
Acc16I TGCGCA 1 cut(s) 481
AccB1I GGYRCC 2 cut(s) 1008, 1406
AccBSI CCGCTC 2 cut(s) 96, 251
AccII CGCG 6 cut(s) 471, 981, 1014, 1109, 1433, 1793
AclWI GGATC 5 cut(s) 500, 917, 1309, 1361, 1949
AcoI YGGCCR 5 cut(s) 41, 867, 1188, 1524, 1959
AcsI RAATTY 3 cut(s) 71, 615, 1576
AcvI CACGTG 2 cut(s) 1413, 1499
AcyI GRCGYC 6 cut(s) 444, 1164, 1269, 1383, 1557, 1928
AfaI GTAC 3 cut(s) 275, 1251, 1472
AfiI CCNNNNNNNGG 7 cut(s) 36, 50, 115, 421, 562, 814, 941
AflIII ACRYGT 2 cut(s) 929, 1498
AgeI ACCGGT 1 cut(s) 872
AgsI TTSAA 3 cut(s) 759, 988, 1576
AhdI GACNNNNNGTC 1 cut(s) 688
AjnI CCWGG 4 cut(s) 114, 1097, 1185, 1526
AjuI GAANNNNNNNTTGG 2 cut(s) 651, 683
Alw26I GTCTC 2 cut(s) 69, 657
AlwI GGATC 5 cut(s) 500, 917, 1309, 1361, 1949
AlwNI CAGNNNCTG 1 cut(s) 952
Ama87I CYCGRG 1 cut(s) 1538
AoxI GGCC 7 cut(s) 41, 718, 867, 954, 1188, 1524, 1959
ApeKI GCWGC 5 cut(s) 141, 283, 324, 1018, 1154
ApoI RAATTY 3 cut(s) 71, 615, 1576
AsiGI ACCGGT 1 cut(s) 872
Asp700I GAANNNNTTC 1 cut(s) 570
AspLEI GCGC 6 cut(s) 473, 482, 755, 983, 1064, 1793
AspS9I GGNCC 4 cut(s) 853, 955, 1279, 1535
AsuC2I CCSGG 4 cut(s) 812, 857, 1539, 1540
AsuHPI GGTGA 6 cut(s) 223, 1309, 1684, 1705, 1918, 1985
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 1538
AvaII GGWCC 3 cut(s) 853, 1279, 1535
BalI TGGCCA 3 cut(s) 869, 1190, 1526
BanI GGYRCC 2 cut(s) 1008, 1406
BauI CACGAG 1 cut(s) 374
BbrPI CACGTG 2 cut(s) 1413, 1499
BbsI GAAGAC 3 cut(s) 216, 315, 447
BbvI GCAGC 5 cut(s) 153, 270, 336, 1030, 1141
BccI CCATC 6 cut(s) 52, 905, 1633, 1702, 1885, 1947
BceAI ACGGC 6 cut(s) 337, 848, 1049, 1177, 1289, 1396
BcgI CGANNNNNNTGC 2 cut(s) 1225, 1259
BciT130I CCWGG 4 cut(s) 116, 1099, 1187, 1528
BclI TGATCA 1 cut(s) 1975
BcnI CCSGG 4 cut(s) 812, 857, 1539, 1540
BcoDI GTCTC 2 cut(s) 69, 657
BfaI CTAG 4 cut(s) 215, 620, 647, 1484
BfmI CTRYAG 3 cut(s) 48, 284, 1292
BfoI RGCGCY 2 cut(s) 756, 1065
BglI GCCNNNNNGGC 1 cut(s) 1565
Bme1390I CCNGG 8 cut(s) 116, 812, 857, 1099, 1187, 1528, 1539, 1540
Bme18I GGWCC 3 cut(s) 853, 1279, 1535
BmeRI GACNNNNNGTC 1 cut(s) 688
BmeT110I CYCGRG 1 cut(s) 1538
BmgT120I GGNCC 4 cut(s) 853, 955, 1279, 1535
BmrFI CCNGG 8 cut(s) 116, 812, 857, 1099, 1187, 1528, 1539, 1540
BmrI ACTGGG 1 cut(s) 1135
BmuI ACTGGG 1 cut(s) 1135
BoxI GACNNNNGTC 1 cut(s) 1272
BpiI GAAGAC 3 cut(s) 216, 315, 447
BpmI CTGGAG 1 cut(s) 1251
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 1966
BpuMI CCSGG 4 cut(s) 812, 857, 1539, 1540
BsaAI YACGTR 3 cut(s) 930, 1413, 1499
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 6 cut(s) 444, 1164, 1269, 1383, 1557, 1928
BsaJI CCNNGG 6 cut(s) 510, 795, 864, 1527, 1538, 1560
BsaWI WCCGGW 3 cut(s) 822, 872, 1660
BsaXI ACNNNNNCTCC 4 cut(s) 1115, 1145, 1838, 1868
Bsc4I CCNNNNNNNGG 7 cut(s) 36, 50, 115, 421, 562, 814, 941
Bse118I RCCGGY 2 cut(s) 872, 1271
Bse1I ACTGG 2 cut(s) 607, 1141
Bse3DI GCAATG 2 cut(s) 1218, 2031
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 4 cut(s) 116, 1099, 1187, 1528
BseDI CCNNGG 6 cut(s) 510, 795, 864, 1527, 1538, 1560
BseGI GGATG 4 cut(s) 342, 520, 1107, 1713
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 7 cut(s) 36, 50, 115, 421, 562, 814, 941
BseMI GCAATG 2 cut(s) 1218, 2031
BseMII CTCAG 1 cut(s) 166
BseNI ACTGG 2 cut(s) 607, 1141
BseRI GAGGAG 7 cut(s) 80, 92, 140, 1713, 1716, 1930, 1963
BseX3I CGGCCG 1 cut(s) 1959
BseXI GCAGC 5 cut(s) 153, 270, 336, 1030, 1141
Bsh1236I CGCG 6 cut(s) 471, 981, 1014, 1109, 1433, 1793
Bsh1285I CGRYCG 5 cut(s) 553, 861, 889, 1276, 1962
BshFI GGCC 7 cut(s) 43, 720, 869, 956, 1190, 1526, 1961
BshNI GGYRCC 2 cut(s) 1008, 1406
BshTI ACCGGT 1 cut(s) 872
BsiEI CGRYCG 5 cut(s) 553, 861, 889, 1276, 1962
BsiHKCI CYCGRG 1 cut(s) 1538
BsiSI CCGG 8 cut(s) 812, 823, 857, 873, 1272, 1539, 1661, 1962
BsiWI CGTACG 1 cut(s) 1249
BslFI GGGAC 8 cut(s) 340, 866, 918, 1292, 1548, 1568, 1909, 1915
BslI CCNNNNNNNGG 7 cut(s) 36, 50, 115, 421, 562, 814, 941
BsmAI GTCTC 2 cut(s) 69, 657
BsmFI GGGAC 8 cut(s) 340, 866, 918, 1292, 1548, 1568, 1909, 1915
BsmI GAATGC 2 cut(s) 20, 1622
BsnI GGCC 7 cut(s) 43, 720, 869, 956, 1190, 1526, 1961
BsoBI CYCGRG 1 cut(s) 1538
Bsp119I TTCGAA 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 1560
BspANI GGCC 7 cut(s) 43, 720, 869, 956, 1190, 1526, 1961
BspCNI CTCAG 1 cut(s) 165
BspFNI CGCG 6 cut(s) 471, 981, 1014, 1109, 1433, 1793
BspMAI CTGCAG 2 cut(s) 52, 288
BspPI GGATC 5 cut(s) 500, 917, 1309, 1361, 1949
BspQI GCTCTTC 1 cut(s) 636
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 2 cut(s) 1008, 1406
BsrBI CCGCTC 2 cut(s) 96, 251
BsrDI GCAATG 2 cut(s) 1218, 2031
BsrFI RCCGGY 2 cut(s) 872, 1271
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 2 cut(s) 607, 1141
BssAI RCCGGY 2 cut(s) 872, 1271
BssECI CCNNGG 6 cut(s) 510, 795, 864, 1527, 1538, 1560
BssNI GRCGYC 6 cut(s) 444, 1164, 1269, 1383, 1557, 1928
BssSI CACGAG 1 cut(s) 374
BssT1I CCWWGG 2 cut(s) 510, 1560
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 4 cut(s) 116, 1099, 1187, 1528
Bst4CI ACNGT 3 cut(s) 477, 1678, 1906
Bst6I CTCTTC 6 cut(s) 209, 319, 631, 636, 752, 1659
BstACI GRCGYC 6 cut(s) 444, 1164, 1269, 1383, 1557, 1928
BstAUI TGTACA 1 cut(s) 273
BstBAI YACGTR 3 cut(s) 930, 1413, 1499
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 5 cut(s) 954, 1016, 1038, 1216, 1467
BstDEI CTNAG 2 cut(s) 152, 838
BstDSI CCRYGG 2 cut(s) 864, 1560
BstENI CCTNNNNNAGG 2 cut(s) 113, 560
BstF5I GGATG 4 cut(s) 342, 520, 1107, 1713
BstFNI CGCG 6 cut(s) 471, 981, 1014, 1109, 1433, 1793
BstH2I RGCGCY 2 cut(s) 756, 1065
BstHHI GCGC 6 cut(s) 473, 482, 755, 983, 1064, 1793
BstMAI GTCTC 2 cut(s) 69, 657
BstMCI CGRYCG 5 cut(s) 553, 861, 889, 1276, 1962
BstNI CCWGG 4 cut(s) 116, 1099, 1187, 1528
BstNSI RCATGY 1 cut(s) 1040
BstPAI GACNNNNGTC 1 cut(s) 1272
BstSCI CCNGG 8 cut(s) 114, 810, 855, 1097, 1185, 1526, 1537, 1538
BstSFI CTRYAG 3 cut(s) 48, 284, 1292
BstUI CGCG 6 cut(s) 471, 981, 1014, 1109, 1433, 1793
BstV1I GCAGC 5 cut(s) 153, 270, 336, 1030, 1141
BstV2I GAAGAC 3 cut(s) 216, 315, 447
BstX2I RGATCY 1 cut(s) 1941
BstYI RGATCY 1 cut(s) 1941
BstZI CGGCCG 1 cut(s) 1959
BsuRI GGCC 7 cut(s) 43, 720, 869, 956, 1190, 1526, 1961
BtgI CCRYGG 2 cut(s) 864, 1560
BtsCI GGATG 4 cut(s) 342, 520, 1107, 1713
BtsIMutI CAGTG 2 cut(s) 482, 1362
Cac8I GCNNGC 5 cut(s) 954, 1016, 1038, 1216, 1467
CaiI CAGNNNCTG 1 cut(s) 952
CfoI GCGC 6 cut(s) 473, 482, 755, 983, 1064, 1793
Cfr10I RCCGGY 2 cut(s) 872, 1271
Cfr13I GGNCC 4 cut(s) 853, 955, 1279, 1535
Cfr9I CCCGGG 1 cut(s) 1538
CseI GACGC 8 cut(s) 503, 958, 1098, 1153, 1277, 1372, 1565, 1917
Csp6I GTAC 3 cut(s) 274, 1250, 1471
CspAI ACCGGT 1 cut(s) 872
CviAII CATG 7 cut(s) 61, 172, 544, 1003, 1037, 1357, 1561
CviQI GTAC 3 cut(s) 274, 1250, 1471
DdeI CTNAG 2 cut(s) 152, 838
DriI GACNNNNNGTC 1 cut(s) 688
EaeI YGGCCR 5 cut(s) 41, 867, 1188, 1524, 1959
EagI CGGCCG 1 cut(s) 1959
Eam1104I CTCTTC 6 cut(s) 209, 319, 631, 636, 752, 1659
Eam1105I GACNNNNNGTC 1 cut(s) 688
EarI CTCTTC 6 cut(s) 209, 319, 631, 636, 752, 1659
EciI GGCGGA 3 cut(s) 515, 830, 1859
EclXI CGGCCG 1 cut(s) 1959
Eco130I CCWWGG 2 cut(s) 510, 1560
Eco47I GGWCC 3 cut(s) 853, 1279, 1535
Eco52I CGGCCG 1 cut(s) 1959
Eco72I CACGTG 2 cut(s) 1413, 1499
Eco88I CYCGRG 1 cut(s) 1538
EcoNI CCTNNNNNAGG 2 cut(s) 113, 560
EcoO109I RGGNCCY 2 cut(s) 853, 1535
EcoRI GAATTC 1 cut(s) 615
EcoRII CCWGG 4 cut(s) 114, 1097, 1185, 1526
EcoT14I CCWWGG 2 cut(s) 510, 1560
ErhI CCWWGG 2 cut(s) 510, 1560
FaeI CATG 7 cut(s) 64, 175, 547, 1006, 1040, 1360, 1564
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 8 cut(s) 340, 866, 918, 1292, 1548, 1568, 1909, 1915
FatI CATG 7 cut(s) 60, 171, 543, 1002, 1036, 1356, 1560
FauI CCCGC 5 cut(s) 33, 146, 843, 1576, 1613
FbaI TGATCA 1 cut(s) 1975
FokI GGATG 4 cut(s) 329, 527, 1114, 1720
FspAI RTGCGCAY 1 cut(s) 481
FspBI CTAG 4 cut(s) 215, 620, 647, 1484
FspI TGCGCA 1 cut(s) 481
GlaI GCGC 6 cut(s) 472, 481, 754, 982, 1063, 1792
GsuI CTGGAG 1 cut(s) 1251
HaeII RGCGCY 2 cut(s) 756, 1065
HaeIII GGCC 7 cut(s) 43, 720, 869, 956, 1190, 1526, 1961
HapII CCGG 8 cut(s) 812, 823, 857, 873, 1272, 1539, 1661, 1962
HgaI GACGC 8 cut(s) 503, 958, 1098, 1153, 1277, 1372, 1565, 1917
HhaI GCGC 6 cut(s) 473, 482, 755, 983, 1064, 1793
Hin1I GRCGYC 6 cut(s) 444, 1164, 1269, 1383, 1557, 1928
Hin1II CATG 7 cut(s) 64, 175, 547, 1006, 1040, 1360, 1564
Hin6I GCGC 6 cut(s) 471, 480, 753, 981, 1062, 1791
HinP1I GCGC 6 cut(s) 471, 480, 753, 981, 1062, 1791
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HindIII AAGCTT 1 cut(s) 188
HpaII CCGG 8 cut(s) 812, 823, 857, 873, 1272, 1539, 1661, 1962
HphI GGTGA 6 cut(s) 223, 1309, 1684, 1705, 1918, 1985
Hpy166II GTNNAC 4 cut(s) 1123, 1171, 1300, 1519
Hpy188III TCNNGA 9 cut(s) 386, 575, 620, 890, 918, 1069, 1277, 1309, 1945
Hpy8I GTNNAC 4 cut(s) 1123, 1171, 1300, 1519
Hpy99I CGWCG 7 cut(s) 449, 461, 686, 1114, 1169, 1315, 1933
HpyAV CCTTC 5 cut(s) 67, 208, 1486, 1760, 1982
HpyCH4III ACNGT 3 cut(s) 477, 1678, 1906
HpyCH4IV ACGT 5 cut(s) 444, 929, 1252, 1412, 1498
HpyCH4V TGCA 4 cut(s) 50, 286, 1218, 1465
HpyF3I CTNAG 2 cut(s) 152, 838
HpySE526I ACGT 5 cut(s) 444, 929, 1252, 1412, 1498
Hsp92I GRCGYC 6 cut(s) 444, 1164, 1269, 1383, 1557, 1928
Hsp92II CATG 7 cut(s) 64, 175, 547, 1006, 1040, 1360, 1564
HspAI GCGC 6 cut(s) 471, 480, 753, 981, 1062, 1791
KflI GGGWCCC 2 cut(s) 853, 1535
Ksp22I TGATCA 1 cut(s) 1975
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 7 cut(s) 53, 424, 471, 545, 799, 1232, 1370
Lsp1109I GCAGC 5 cut(s) 153, 270, 336, 1030, 1141
MaeI CTAG 4 cut(s) 215, 620, 647, 1484
MaeII ACGT 5 cut(s) 444, 929, 1252, 1412, 1498
MaeIII GTNAC 3 cut(s) 1306, 1672, 1807
MbiI CCGCTC 2 cut(s) 96, 251
MfeI CAATTG 2 cut(s) 701, 1716
MflI RGATCY 1 cut(s) 1941
MlsI TGGCCA 3 cut(s) 869, 1190, 1526
MluCI AATT 7 cut(s) 71, 615, 701, 1576, 1716, 1812, 1886
MluNI TGGCCA 3 cut(s) 869, 1190, 1526
MlyI GAGTC 7 cut(s) 169, 305, 362, 376, 617, 659, 902
MmeI TCCRAC 4 cut(s) 102, 1662, 1752, 1812
Mox20I TGGCCA 3 cut(s) 869, 1190, 1526
MroXI GAANNNNTTC 1 cut(s) 570
MscI TGGCCA 3 cut(s) 869, 1190, 1526
MseI TTAA 2 cut(s) 429, 1376
MslI CAYNNNNRTG 2 cut(s) 1100, 1391
Msp20I TGGCCA 3 cut(s) 869, 1190, 1526
MspI CCGG 8 cut(s) 812, 823, 857, 873, 1272, 1539, 1661, 1962
MspR9I CCNGG 8 cut(s) 116, 812, 857, 1099, 1187, 1528, 1539, 1540
MunI CAATTG 2 cut(s) 701, 1716
Mva1269I GAATGC 2 cut(s) 20, 1622
MvaI CCWGG 4 cut(s) 116, 1099, 1187, 1528
MvnI CGCG 6 cut(s) 471, 981, 1014, 1109, 1433, 1793
NciI CCSGG 4 cut(s) 812, 857, 1539, 1540
NcoI CCATGG 1 cut(s) 1560
NlaIII CATG 7 cut(s) 64, 175, 547, 1006, 1040, 1360, 1564
NmeAIII GCCGAG 2 cut(s) 1066, 1235
NmuCI GTSAC 3 cut(s) 1306, 1672, 1807
NsbI TGCGCA 1 cut(s) 481
NspI RCATGY 1 cut(s) 1040
NspV TTCGAA 1 cut(s) 207
PaeI GCATGC 1 cut(s) 1040
PciSI GCTCTTC 1 cut(s) 636
PcsI WCGNNNNNNNCGW 6 cut(s) 444, 453, 1038, 1409, 1778, 1910
PctI GAATGC 2 cut(s) 20, 1622
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 6 cut(s) 134, 291, 914, 1023, 1199, 1237
Pfl23II CGTACG 1 cut(s) 1249
PflFI GACNNNGTC 2 cut(s) 857, 1108
PinAI ACCGGT 1 cut(s) 872
Ple19I CGATCG 2 cut(s) 553, 889
PleI GAGTC 7 cut(s) 169, 305, 361, 376, 617, 658, 901
PmaCI CACGTG 2 cut(s) 1413, 1499
PmlI CACGTG 2 cut(s) 1413, 1499
PpsI GAGTC 7 cut(s) 169, 305, 361, 376, 617, 658, 901
Ppu21I YACGTR 3 cut(s) 930, 1413, 1499
PpuMI RGGWCCY 2 cut(s) 853, 1535
PshAI GACNNNNGTC 1 cut(s) 1272
Psp5II RGGWCCY 2 cut(s) 853, 1535
Psp6I CCWGG 4 cut(s) 114, 1097, 1185, 1526
PspCI CACGTG 2 cut(s) 1413, 1499
PspGI CCWGG 4 cut(s) 114, 1097, 1185, 1526
PspLI CGTACG 1 cut(s) 1249
PspPI GGNCC 4 cut(s) 853, 955, 1279, 1535
PspPPI RGGWCCY 2 cut(s) 853, 1535
PstI CTGCAG 2 cut(s) 52, 288
PstNI CAGNNNCTG 1 cut(s) 952
PsuI RGATCY 1 cut(s) 1941
PsyI GACNNNGTC 2 cut(s) 857, 1108
PvuI CGATCG 2 cut(s) 553, 889
RsaI GTAC 3 cut(s) 275, 1251, 1472
RsaNI GTAC 3 cut(s) 274, 1250, 1471
RseI CAYNNNNRTG 2 cut(s) 1100, 1391
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 2 cut(s) 429, 1376
Sau96I GGNCC 4 cut(s) 853, 955, 1279, 1535
SchI GAGTC 7 cut(s) 169, 305, 362, 376, 617, 659, 902
ScrFI CCNGG 8 cut(s) 116, 812, 857, 1099, 1187, 1528, 1539, 1540
SfcI CTRYAG 3 cut(s) 48, 284, 1292
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 3 cut(s) 853, 1279, 1535
SmaI CCCGGG 1 cut(s) 1540
SmiMI CAYNNNNRTG 2 cut(s) 1100, 1391
SmlI CTYRAG 1 cut(s) 1945
SmoI CTYRAG 1 cut(s) 1945
SphI GCATGC 1 cut(s) 1040
Sse9I AATT 7 cut(s) 71, 615, 701, 1576, 1716, 1812, 1886
SspMI CTAG 4 cut(s) 215, 620, 647, 1484
StyD4I CCNGG 8 cut(s) 114, 810, 855, 1097, 1185, 1526, 1537, 1538
StyI CCWWGG 2 cut(s) 510, 1560
TaaI ACNGT 3 cut(s) 477, 1678, 1906
TaiI ACGT 5 cut(s) 447, 932, 1255, 1415, 1501
TasI AATT 7 cut(s) 71, 615, 701, 1576, 1716, 1812, 1886
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 6 cut(s) 134, 291, 914, 1023, 1199, 1237
Tru1I TTAA 2 cut(s) 429, 1376
Tru9I TTAA 2 cut(s) 429, 1376
TscAI CASTG 2 cut(s) 482, 1369
TseFI GTSAC 3 cut(s) 1306, 1672, 1807
TseI GCWGC 5 cut(s) 141, 283, 324, 1018, 1154
Tsp45I GTSAC 3 cut(s) 1306, 1672, 1807
TspDTI ATGAA 2 cut(s) 49, 2001
TspGWI ACGGA 1 cut(s) 1383
TspMI CCCGGG 1 cut(s) 1538
TspRI CASTG 2 cut(s) 482, 1369
Tth111I GACNNNGTC 2 cut(s) 857, 1108
VpaK11BI GGWCC 3 cut(s) 853, 1279, 1535
XagI CCTNNNNNAGG 2 cut(s) 113, 560
XapI RAATTY 3 cut(s) 71, 615, 1576
XbaI TCTAGA 1 cut(s) 619
XceI RCATGY 1 cut(s) 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 700
XmaI CCCGGG 1 cut(s) 1538
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 4 cut(s) 215, 620, 647, 1484
ZraI GACGTC 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.