FvH4_5g30861

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012625
Physical Location & Seq
Reverse (-)
21727053 .. 21731011
3959 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30861.t2

Sequence Viewer

Length: 1437 bp
ATGGGGGCTATATTGAGTTTGAATAATTCAAGGACTAGAGAGAGTGGACCTCATGGGGTTACGCAGAGTGAAGCACGCAAGAGGCAGAGGTTGTCTTCATTCCTTTATGAGGAGAACCCGAGATTGATTCCCAGTCTTCCTGACGAGATATCTATTCAGATTCTTGCCAGGGTACCTAGGATCCACTATTTGAAAATGAAGATGGTGTCACATGCTTGGAAAACCACTGTTATGAGTGCTGAGCTTTTCAATTTGAGAAAAGAGCTAGGAACAACGGAGGAATGGCTCTATGTGTTAACAAAGGTTGAAAGTGATAAGCTCATATGGAATGCTCTGGACCCTCTGGCCGGAAGATGGCAAAGGTTGCCACCAATGCCTAGTGTTTGTCCTGAAGATGAATCTAGGAGGGCTTTAACTGCCCGTAGAATGTGGAAAATGGTAGGCTCAAGCATTAGACTTGCGGATGTTATCATGGGATGGCTTGGGAGGAAAGATGTGCTGGATCGAATGCCTTTTTGTGGTTGTGCCATTGGTGCTGTTGATGGCTGCCTCTATGTCCTAGGTGGATTTTCAAGGGCTACAGCCCTATCATGTGCCTGGCGGTATAACCCAGTTAATAACTCTTGGAGTGAATCAGCTCCGATGTCAATGGGTAGAGCGTATTGTAAGACAGGCGTTTTAAACAATAAACTTTATGTTGTTGGAGGGGTTACTAGAGGACGTGGTGGATTGGCCCCTCTTCAATCAGCTGAAGTATTTGATCCAAAAACAGGTCTATGGTCCCAAATACCAAGCATGCCCTTTACCAAAGCTCAGGTGTTACCAACTGCATTTTTGACTGATCTGCTCAAGCCTATTGCTACCGGGATGACTTCATACAAGGGTAGGTTGTTTGTTCCACAAAGTTTATACTGCTGGCCCTTTTTTGTTGACGTTGGAGGTGAGGTGTATGATCCTAAGGTGAATTCATGGGGTGAAATGCCAACTGGCATGGGAGAGGGTTGGCCCGCAAGGCAAGCAGGGACGAAAATGAGTGTCACAGTTGATGGTGAATTGTATGCACTGGATCCTTCTAGTTCTCTTGATAGTGCCAAGATCAAGTTATATGATTATCAAGATGATGCTTGGAAAATTGTTGCAGACGACGTTCCAATTCGGGACTTCACTGAATCAGAGTCTCCTTATTTACTTGCCGGTTTCCTTGGGAAGCTTCATGTGATCACGAAGGATGCGAGTAACAATGTTGCAGTTCTGCAGGCATATGTGCCGAATCGTCTTGCTTCCATCTCAACAGATTCATCCTCTTCCTCATCCCAAGACAATGCCTCTGAAATTTCAGAACAAAAAGCAGAATCAGAAGTAGATCTTTGGAAGGTAATTGCCACAAGGAGTGCACGAACTACTGAACTGGTTAGCTGTCTCGTCCTTGATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

479

Amino Acids

52.93

Weight (kDa)

8.01

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 82 2.1e-06 F-box domain
Kelch_FKB95 PF25210 169 - 265 2.1e-11 FKB95, Kelch-repeats domain
Kelch_1 PF01344 171 - 216 7.9e-14 Kelch motif
Beta-prop_Calicin PF13964 177 - 269 9.4e-09 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 177 - 266 2.1e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 219 - 267 1e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 172
AciI CCGC 3 cut(s) 461, 601, 1008
AclWI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 964, 1332
AcuI CTGAAG 2 cut(s) 411, 771
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 5 cut(s) 109, 347, 354, 518, 770
AgsI TTSAA 7 cut(s) 22, 30, 193, 250, 308, 573, 743
AjiI CACGTC 1 cut(s) 722
AjnI CCWGG 2 cut(s) 167, 596
AluBI AGCT 8 cut(s) 244, 265, 319, 638, 749, 812, 1210, 1416
AluI AGCT 8 cut(s) 244, 265, 319, 638, 749, 812, 1210, 1416
Alw21I GWGCWC 1 cut(s) 1396
Alw26I GTCTC 2 cut(s) 1182, 1424
Alw44I GTGCAC 1 cut(s) 1392
AlwI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 4 cut(s) 345, 732, 917, 1004
ApaLI GTGCAC 1 cut(s) 1392
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 2 cut(s) 964, 1332
Asp718I GGTACC 1 cut(s) 172
AspA2I CCTAGG 2 cut(s) 176, 559
AspS9I GGNCC 6 cut(s) 47, 337, 733, 780, 918, 1005
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 4 cut(s) 953, 973, 986, 1061
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 3 cut(s) 47, 337, 780
AvrII CCTAGG 2 cut(s) 176, 559
AxyI CCTNAGG 1 cut(s) 957
BaeGI GKGCMC 1 cut(s) 1396
BamHI GGATCC 2 cut(s) 180, 1066
BanI GGYRCC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 87, 128
Bbv12I GWGCWC 1 cut(s) 1396
BbvI GCAGC 1 cut(s) 533
BccI CCATC 6 cut(s) 196, 348, 471, 536, 1040, 1292
BciT130I CCWGG 2 cut(s) 169, 598
BclI TGATCA 1 cut(s) 1218
BcnI CCSGG 1 cut(s) 865
BcoDI GTCTC 2 cut(s) 1182, 1424
BfaI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
BfmI CTRYAG 2 cut(s) 579, 1253
BglI GCCNNNNNGGC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 1363
BisI GCNGC 1 cut(s) 547
BlnI CCTAGG 2 cut(s) 176, 559
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 548
Bme1390I CCNGG 3 cut(s) 169, 598, 865
Bme18I GGWCC 3 cut(s) 47, 337, 780
BmeT110I CYCGRG 1 cut(s) 118
BmgBI CACGTC 1 cut(s) 722
BmgT120I GGNCC 6 cut(s) 47, 337, 733, 780, 918, 1005
BmiI GGNNCC 6 cut(s) 174, 182, 339, 735, 782, 1068
BmrFI CCNGG 3 cut(s) 169, 598, 865
BmrI ACTGGG 2 cut(s) 126, 605
BmsI GCATC 2 cut(s) 1111, 1219
BmuI ACTGGG 2 cut(s) 126, 605
BpiI GAAGAC 2 cut(s) 87, 128
BplI GAGNNNNNCTC 2 cut(s) 34, 66
Bpu10I CCTNAGC 1 cut(s) 813
Bpu1102I GCTNAGC 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 430, 833
BpuMI CCSGG 1 cut(s) 865
BsaJI CCNNGG 4 cut(s) 168, 176, 559, 1201
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 347, 354, 518, 770
Bse118I RCCGGY 1 cut(s) 1193
Bse1I ACTGG 5 cut(s) 132, 611, 991, 1068, 1413
Bse21I CCTNAGG 1 cut(s) 957
BseBI CCWGG 2 cut(s) 169, 598
BseDI CCNNGG 4 cut(s) 168, 176, 559, 1201
BseGI GGATG 6 cut(s) 469, 482, 873, 1234, 1298, 1310
BseLI CCNNNNNNNGG 5 cut(s) 109, 347, 354, 518, 770
BseMII CTCAG 2 cut(s) 231, 827
BseNI ACTGG 5 cut(s) 132, 611, 991, 1068, 1413
BseRI GAGGAG 1 cut(s) 125
BseSI GKGCMC 1 cut(s) 1396
BseXI GCAGC 1 cut(s) 533
BshFI GGCC 4 cut(s) 347, 734, 919, 1006
BshNI GGYRCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 1396
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 3 cut(s) 348, 864, 1194
BslFI GGGAC 3 cut(s) 766, 1036, 1172
BslI CCNNNNNNNGG 5 cut(s) 109, 347, 354, 518, 770
BsmAI GTCTC 2 cut(s) 1182, 1424
BsmFI GGGAC 3 cut(s) 766, 1036, 1172
BsmI GAATGC 2 cut(s) 334, 513
BsnI GGCC 4 cut(s) 347, 734, 919, 1006
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 1396
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 3 cut(s) 461, 601, 1008
BspANI GGCC 4 cut(s) 347, 734, 919, 1006
BspCNI CTCAG 2 cut(s) 232, 826
BspLI GGNNCC 6 cut(s) 174, 182, 339, 735, 782, 1068
BspMAI CTGCAG 1 cut(s) 1257
BspPI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
BspT107I GGYRCC 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 1193
BsrI ACTGG 5 cut(s) 132, 611, 991, 1068, 1413
BssAI RCCGGY 1 cut(s) 1193
BssECI CCNNGG 4 cut(s) 168, 176, 559, 1201
BssT1I CCWWGG 3 cut(s) 176, 559, 1201
Bst2UI CCWGG 2 cut(s) 169, 598
Bst4CI ACNGT 2 cut(s) 229, 1042
Bst6I CTCTTC 2 cut(s) 744, 1309
BstAPI GCANNNNNTGC 1 cut(s) 364
BstC8I GCNNGC 6 cut(s) 76, 797, 917, 1008, 1017, 1257
BstDEI CTNAG 3 cut(s) 240, 813, 957
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 6 cut(s) 469, 482, 873, 1234, 1298, 1310
BstMAI GTCTC 2 cut(s) 1182, 1424
BstMWI GCNNNNNNNGC 6 cut(s) 364, 373, 416, 533, 1012, 1016
BstNI CCWGG 2 cut(s) 169, 598
BstNSI RCATGY 2 cut(s) 215, 799
BstSCI CCNGG 3 cut(s) 167, 596, 863
BstSFI CTRYAG 2 cut(s) 579, 1253
BstSLI GKGCMC 1 cut(s) 1396
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 2 cut(s) 87, 128
BstX2I RGATCY 3 cut(s) 180, 1066, 1363
BstYI RGATCY 3 cut(s) 180, 1066, 1363
Bsu36I CCTNAGG 1 cut(s) 957
BsuRI GGCC 4 cut(s) 347, 734, 919, 1006
BtrI CACGTC 1 cut(s) 722
BtsCI GGATG 6 cut(s) 469, 482, 873, 1234, 1298, 1310
BtsIMutI CAGTG 3 cut(s) 225, 1061, 1164
Cac8I GCNNGC 6 cut(s) 76, 797, 917, 1008, 1017, 1257
Cfr10I RCCGGY 1 cut(s) 1193
Cfr13I GGNCC 6 cut(s) 47, 337, 733, 780, 918, 1005
Csp6I GTAC 1 cut(s) 173
CviAII CATG 8 cut(s) 53, 212, 472, 591, 796, 969, 991, 1214
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 3 cut(s) 240, 813, 957
DraI TTTAAA 1 cut(s) 681
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 744, 1309
EarI CTCTTC 2 cut(s) 744, 1309
Eco130I CCWWGG 3 cut(s) 176, 559, 1201
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 3 cut(s) 47, 337, 780
Eco57I CTGAAG 2 cut(s) 411, 771
Eco81I CCTNAGG 1 cut(s) 957
Eco88I CYCGRG 1 cut(s) 118
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 2 cut(s) 167, 596
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 3 cut(s) 176, 559, 1201
ErhI CCWWGG 3 cut(s) 176, 559, 1201
FaeI CATG 8 cut(s) 56, 215, 475, 594, 799, 972, 994, 1217
FalI AAGNNNNNCTT 2 cut(s) 1350, 1382
FaqI GGGAC 3 cut(s) 766, 1036, 1172
FatI CATG 8 cut(s) 52, 211, 471, 590, 795, 968, 990, 1213
FauI CCCGC 1 cut(s) 1015
FauNDI CATATG 2 cut(s) 323, 1261
FbaI TGATCA 1 cut(s) 1218
Fnu4HI GCNGC 1 cut(s) 547
FokI GGATG 6 cut(s) 476, 489, 880, 1241, 1285, 1297
Fsp4HI GCNGC 1 cut(s) 547
FspBI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
GluI GCNGC 1 cut(s) 547
HaeIII GGCC 4 cut(s) 347, 734, 919, 1006
HapII CCGG 3 cut(s) 348, 864, 1194
Hin1II CATG 8 cut(s) 56, 215, 475, 594, 799, 972, 994, 1217
HincII GTYRAC 2 cut(s) 297, 931
HindII GTYRAC 2 cut(s) 297, 931
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 9 cut(s) 127, 160, 398, 632, 1169, 1175, 1270, 1295, 1352
HpaI GTTAAC 1 cut(s) 297
HpaII CCGG 3 cut(s) 348, 864, 1194
HphI GGTGA 4 cut(s) 953, 973, 986, 1061
Hpy166II GTNNAC 4 cut(s) 47, 297, 931, 1394
Hpy188I TCNGA 6 cut(s) 159, 642, 1174, 1330, 1339, 1357
Hpy188III TCNNGA 7 cut(s) 140, 335, 389, 1082, 1115, 1157, 1222
Hpy8I GTNNAC 4 cut(s) 47, 297, 931, 1394
Hpy99I CGWCG 1 cut(s) 1148
HpyAV CCTTC 3 cut(s) 1080, 1219, 1366
HpyCH4III ACNGT 2 cut(s) 229, 1042
HpyCH4IV ACGT 3 cut(s) 721, 933, 1146
HpyCH4V TGCA 6 cut(s) 830, 1061, 1139, 1247, 1255, 1394
HpyF10VI GCNNNNNNNGC 6 cut(s) 364, 373, 416, 533, 1012, 1016
HpyF3I CTNAG 3 cut(s) 240, 813, 957
HpySE526I ACGT 3 cut(s) 721, 933, 1146
Hsp92II CATG 8 cut(s) 56, 215, 475, 594, 799, 972, 994, 1217
KpnI GGTACC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 1218
KspAI GTTAAC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 643
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 2 cut(s) 1111, 1219
MaeI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
MaeII ACGT 3 cut(s) 721, 933, 1146
MaeIII GTNAC 6 cut(s) 58, 207, 709, 819, 1036, 1235
MboII GAAGA 7 cut(s) 87, 128, 211, 363, 404, 731, 1296
MflI RGATCY 3 cut(s) 180, 1066, 1363
MhlI GDGCHC 1 cut(s) 1396
MluCI AATT 8 cut(s) 25, 250, 964, 1052, 1131, 1152, 1332, 1377
MlyI GAGTC 1 cut(s) 1184
MmeI TCCRAC 2 cut(s) 682, 916
MseI TTAA 4 cut(s) 296, 413, 615, 680
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 3 cut(s) 348, 864, 1194
MspR9I CCNGG 3 cut(s) 169, 598, 865
Mva1269I GAATGC 2 cut(s) 334, 513
MvaI CCWGG 2 cut(s) 169, 598
MwoI GCNNNNNNNGC 6 cut(s) 364, 373, 416, 533, 1012, 1016
NciI CCSGG 1 cut(s) 865
NdeI CATATG 2 cut(s) 323, 1261
NlaIII CATG 8 cut(s) 56, 215, 475, 594, 799, 972, 994, 1217
NlaIV GGNNCC 6 cut(s) 174, 182, 339, 735, 782, 1068
NmuCI GTSAC 2 cut(s) 207, 1036
NspI RCATGY 2 cut(s) 215, 799
PaeI GCATGC 1 cut(s) 799
PcsI WCGNNNNNNNCGW 1 cut(s) 1229
PctI GAATGC 2 cut(s) 334, 513
PfeI GAWTC 8 cut(s) 127, 160, 398, 632, 1169, 1270, 1295, 1352
PkrI GCNGC 1 cut(s) 548
PleI GAGTC 1 cut(s) 1183
PpsI GAGTC 1 cut(s) 1183
Psp6I CCWGG 2 cut(s) 167, 596
PspGI CCWGG 2 cut(s) 167, 596
PspN4I GGNNCC 6 cut(s) 174, 182, 339, 735, 782, 1068
PspPI GGNCC 6 cut(s) 47, 337, 733, 780, 918, 1005
PstI CTGCAG 1 cut(s) 1257
PsuI RGATCY 3 cut(s) 180, 1066, 1363
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 4 cut(s) 296, 413, 615, 680
SatI GCNGC 1 cut(s) 547
Sau96I GGNCC 6 cut(s) 47, 337, 733, 780, 918, 1005
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 3 cut(s) 169, 598, 865
SduI GDGCHC 1 cut(s) 1396
SfaNI GCATC 2 cut(s) 1111, 1219
SfcI CTRYAG 2 cut(s) 579, 1253
SinI GGWCC 3 cut(s) 47, 337, 780
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 2 cut(s) 445, 848
SmoI CTYRAG 2 cut(s) 445, 848
SphI GCATGC 1 cut(s) 799
Sse9I AATT 8 cut(s) 25, 250, 964, 1052, 1131, 1152, 1332, 1377
SsiI CCGC 3 cut(s) 461, 601, 1008
SspMI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
StyD4I CCNGG 3 cut(s) 167, 596, 863
StyI CCWWGG 3 cut(s) 176, 559, 1201
TaaI ACNGT 2 cut(s) 229, 1042
TaiI ACGT 3 cut(s) 724, 936, 1149
TaqI TCGA 1 cut(s) 505
TasI AATT 8 cut(s) 25, 250, 964, 1052, 1131, 1152, 1332, 1377
TfiI GAWTC 8 cut(s) 127, 160, 398, 632, 1169, 1270, 1295, 1352
Tru1I TTAA 4 cut(s) 296, 413, 615, 680
Tru9I TTAA 4 cut(s) 296, 413, 615, 680
TscAI CASTG 3 cut(s) 232, 1068, 1171
TseFI GTSAC 2 cut(s) 207, 1036
TseI GCWGC 1 cut(s) 546
Tsp45I GTSAC 2 cut(s) 207, 1036
TspDTI ATGAA 7 cut(s) 87, 212, 411, 864, 957, 1202, 1287
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 3 cut(s) 232, 1068, 1171
VneI GTGCAC 1 cut(s) 1392
VpaK11BI GGWCC 3 cut(s) 47, 337, 780
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 2 cut(s) 964, 1332
XceI RCATGY 2 cut(s) 215, 799
XmaJI CCTAGG 2 cut(s) 176, 559
XspI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.