pycom08g19200

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012625
Physical Location & Seq
Forward (+)
19227101 .. 19228531
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19200.1

Sequence Viewer

Length: 1431 bp
ATGGGGGCTAGATTGAGCTTGAACCATTCCAAGACTAGGGTCGGTGAACCGTCTCAAGATTTACGTAGTGCATCTTGCAAGAGGCCGAGGTTGTCCCAAAGCTTTTACGACGAAAACCCAAGATTGATTCCCTGCCTTCCTGACGAGATATCTATTCAGATTCTTGCTAGAATTCCTAGGATCCACTATATGAAGCTAAAGTCTGTTTCGAGGACCTGGAAAGCCACCGTTACAAGTGCTGAACTCTTCTCTTTGAGAAAAGAGCTAGGAACAACGGAGGAATGGCTCTACATGTTAACAAAGGTCCAAAATGATAAGCTCATATGGTATGCACTTGATCCTATGGCCGGAAGATGGCAAAGGTTGCCACCTATGCCTAATGTTACTCTGGAAGATGAATCTAGGAAAGGTTTAACTGGCCAGAGATTGTGGAATATGGCAGGCTCCAGTATCAGAATTGCGGATGCCGTCATGGGTTGGCTTGGGAGGAAAGATTCACTAGATCGAATGCCTTTTTGTGGTTGCTCTATTGGGGCTATTGATGGCTGCCTCTATGTGCTAGGGGGATTTTCCAAGGCTTCGGCCCTGAGATGCACATGGCGGTACAATCCAGTTAAAAATACTTGGAGTGAATCAAGTCCAATGTCAATTGGTAGAGCTTATTGTAAGACCGGCATCTTAAACGATAAGCTTTATGTTGTTGGAGGTGTTACAAGGGGTCATGGTGGATTGACCCCTCTTCAATCAGCTGAAGTATTTGATCCAAAAATAGGTGTGTGGTCTCAAATACCAAGCATGCCTTTTATGAAAGCTCAGGTGTTACCAACTGCATTTTTGGCTGATCTGCTGAAGCCTATTGCTACCGGAATGACTTCATACAGGGGAAGGTTGTTCGTTCCCCAGAGTTTGTACTGCTGGCCATTTTTTGTTGATGTTGGAGGAGAGGTTTATGATCCAGAGGCAAATTCATGGGTTGAAATGCCAATTGGTATGGGCGAGGGTTGGCCTGCGAGGCAGGCAGGGACGAAAATGAGTGTCACAGTTGATGGCGAATTGTATGCACTGGATCCTTCTAGTTCTCTTGACAGTGCAAAGATCAAGGTATACGATTACCAAGCTGATGCCTGGAAGGTTGTTGCAGAAGATGTTCCAATTCGTGACTTCACTGAATCAGAGTCTCCCTATTTGCTTGCTGGATTGCTGGGGAAGCTTCATGTAATCACAAAGGATGCAAATCACAAAATTGCTGTTCTGCAGGCTTCAGTGCAGGACCATTTTGTGTCAAGCTTAGTAGCTTCATCCTCATCACACAATGACCCCCATGAAGATGTTGAACGACCAGCAGAATCAGAACGAGATCTCTGGAAAGTAATTGCCACAAGGAGTGCACGGGCTGCTGAACTGGTTAGTTGTCTAGTCCTTGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

477

Amino Acids

52.89

Weight (kDa)

7.99

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 83 1.5e-07 F-box domain
Kelch_FKB95 PF25210 166 - 409 2.8e-13 FKB95, Kelch-repeats domain
Kelch_1 PF01344 171 - 216 8.6e-13 Kelch motif
Kelch_2 PF07646 171 - 215 4.7e-06 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 174 - 266 3.2e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 176 - 268 8.2e-07 Calicin, beta-propeller domain
Kelch_1 PF01344 219 - 267 4.5e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1104
AciI CCGC 2 cut(s) 461, 601
AclWI GGATC 7 cut(s) 175, 188, 332, 755, 947, 1061, 1074
AcoI YGGCCR 3 cut(s) 345, 418, 917
AcsI RAATTY 2 cut(s) 171, 964
AcuI CTGAAG 3 cut(s) 771, 869, 1245
AfaI GTAC 2 cut(s) 605, 911
AfiI CCNNNNNNNGG 5 cut(s) 36, 347, 354, 518, 770
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 4 cut(s) 22, 743, 977, 1334
AjnI CCWGG 2 cut(s) 215, 1124
AjuI GAANNNNNNNTTGG 2 cut(s) 969, 1001
Alw21I GWGCWC 1 cut(s) 1390
Alw26I GTCTC 3 cut(s) 57, 786, 1182
Alw44I GTGCAC 1 cut(s) 1386
AlwI GGATC 7 cut(s) 175, 188, 332, 755, 947, 1061, 1074
AoxI GGCC 6 cut(s) 83, 345, 418, 582, 917, 1004
ApaLI GTGCAC 1 cut(s) 1386
ApeKI GCWGC 2 cut(s) 546, 1394
ApoI RAATTY 2 cut(s) 171, 964
Asp700I GAANNNNTTC 2 cut(s) 871, 1146
AspA2I CCTAGG 1 cut(s) 176
AspS9I GGNCC 4 cut(s) 213, 304, 583, 1270
AsuHPI GGTGA 1 cut(s) 56
AvaII GGWCC 3 cut(s) 213, 304, 1270
AvrII CCTAGG 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 1390
BalI TGGCCA 2 cut(s) 420, 919
BamHI GGATCC 2 cut(s) 180, 1066
Bbv12I GWGCWC 1 cut(s) 1390
BbvI GCAGC 2 cut(s) 533, 1381
BccI CCATC 3 cut(s) 348, 536, 1040
BceAI ACGGC 1 cut(s) 452
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 2 cut(s) 217, 1126
BcoDI GTCTC 3 cut(s) 57, 786, 1182
BfmI CTRYAG 1 cut(s) 1253
BglI GCCNNNNNGGC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 1357
BisI GCNGC 2 cut(s) 547, 1395
BlnI CCTAGG 1 cut(s) 176
BlsI GCNGC 2 cut(s) 548, 1396
Bme1390I CCNGG 2 cut(s) 217, 1126
Bme18I GGWCC 3 cut(s) 213, 304, 1270
BmgT120I GGNCC 4 cut(s) 213, 304, 583, 1270
BmiI GGNNCC 3 cut(s) 182, 445, 1068
BmrFI CCNGG 2 cut(s) 217, 1126
BmsI GCATC 6 cut(s) 80, 454, 581, 684, 1111, 1219
BoxI GACNNNNGTC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 813
BpuEI CTTGAG 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 1233
BsaI GGTCTC 1 cut(s) 786
BsaJI CCNNGG 3 cut(s) 86, 176, 573
BsaWI WCCGGW 1 cut(s) 863
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 5 cut(s) 36, 347, 354, 518, 770
Bse118I RCCGGY 1 cut(s) 671
Bse1I ACTGG 5 cut(s) 421, 447, 611, 1068, 1407
Bse8I GATNNNNATC 1 cut(s) 1233
BseBI CCWGG 2 cut(s) 217, 1126
BseDI CCNNGG 3 cut(s) 86, 176, 573
BseGI GGATG 3 cut(s) 469, 1234, 1298
BseJI GATNNNNATC 1 cut(s) 1233
BseLI CCNNNNNNNGG 5 cut(s) 36, 347, 354, 518, 770
BseMII CTCAG 2 cut(s) 578, 827
BseNI ACTGG 5 cut(s) 421, 447, 611, 1068, 1407
BseRI GAGGAG 1 cut(s) 954
BseSI GKGCMC 1 cut(s) 1390
BseXI GCAGC 2 cut(s) 533, 1381
BseYI CCCAGC 1 cut(s) 1201
BsgI GTGCAG 1 cut(s) 1286
BshFI GGCC 6 cut(s) 85, 347, 420, 584, 919, 1006
BsiHKAI GWGCWC 1 cut(s) 1390
BsiSI CCGG 3 cut(s) 348, 672, 864
BslFI GGGAC 2 cut(s) 79, 1036
BslI CCNNNNNNNGG 5 cut(s) 36, 347, 354, 518, 770
BsmAI GTCTC 3 cut(s) 57, 786, 1182
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 79, 1036
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 6 cut(s) 85, 347, 420, 584, 919, 1006
Bso31I GGTCTC 1 cut(s) 786
Bsp1286I GDGCHC 1 cut(s) 1390
Bsp143I GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
BspACI CCGC 2 cut(s) 461, 601
BspANI GGCC 6 cut(s) 85, 347, 420, 584, 919, 1006
BspCNI CTCAG 2 cut(s) 579, 826
BspLI GGNNCC 3 cut(s) 182, 445, 1068
BspMAI CTGCAG 1 cut(s) 1257
BspPI GGATC 7 cut(s) 175, 188, 332, 755, 947, 1061, 1074
BspTNI GGTCTC 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 671
BsrI ACTGG 5 cut(s) 421, 447, 611, 1068, 1407
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 3 cut(s) 86, 176, 573
BssMI GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
BssNAI GTATAC 1 cut(s) 1105
BssT1I CCWWGG 2 cut(s) 176, 573
Bst1107I GTATAC 1 cut(s) 1105
Bst2UI CCWGG 2 cut(s) 217, 1126
Bst4CI ACNGT 4 cut(s) 51, 229, 1042, 1088
Bst6I CTCTTC 2 cut(s) 251, 744
BstAPI GCANNNNNTGC 2 cut(s) 364, 1394
BstBAI YACGTR 1 cut(s) 65
BstC8I GCNNGC 7 cut(s) 442, 797, 917, 1008, 1017, 1191, 1257
BstDEI CTNAG 3 cut(s) 587, 813, 1288
BstF5I GGATG 3 cut(s) 469, 1234, 1298
BstKTI GATC 9 cut(s) 183, 340, 505, 763, 844, 955, 1069, 1098, 1360
BstMAI GTCTC 3 cut(s) 57, 786, 1182
BstMBI GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
BstMWI GCNNNNNNNGC 7 cut(s) 364, 373, 836, 1012, 1016, 1207, 1394
BstNI CCWGG 2 cut(s) 217, 1126
BstNSI RCATGY 2 cut(s) 295, 799
BstPAI GACNNNNGTC 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 215, 1124
BstSFI CTRYAG 1 cut(s) 1253
BstSLI GKGCMC 1 cut(s) 1390
BstSNI TACGTA 1 cut(s) 65
BstV1I GCAGC 2 cut(s) 533, 1381
BstX2I RGATCY 3 cut(s) 180, 1066, 1357
BstYI RGATCY 3 cut(s) 180, 1066, 1357
BstZ17I GTATAC 1 cut(s) 1105
BsuRI GGCC 6 cut(s) 85, 347, 420, 584, 919, 1006
BtsCI GGATG 3 cut(s) 469, 1234, 1298
BtsIMutI CAGTG 4 cut(s) 1061, 1093, 1164, 1269
Cac8I GCNNGC 7 cut(s) 442, 797, 917, 1008, 1017, 1191, 1257
Cfr10I RCCGGY 1 cut(s) 671
Cfr13I GGNCC 4 cut(s) 213, 304, 583, 1270
Csp6I GTAC 2 cut(s) 604, 910
CviAII CATG 8 cut(s) 292, 472, 597, 722, 796, 969, 1214, 1322
CviQI GTAC 2 cut(s) 604, 910
DdeI CTNAG 3 cut(s) 587, 813, 1288
DpnI GATC 9 cut(s) 182, 339, 504, 762, 843, 954, 1068, 1097, 1359
DpnII GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
EaeI YGGCCR 3 cut(s) 345, 418, 917
Eam1104I CTCTTC 2 cut(s) 251, 744
EarI CTCTTC 2 cut(s) 251, 744
Eco105I TACGTA 1 cut(s) 65
Eco130I CCWWGG 2 cut(s) 176, 573
Eco31I GGTCTC 1 cut(s) 786
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 3 cut(s) 213, 304, 1270
Eco57I CTGAAG 3 cut(s) 771, 869, 1245
EcoO109I RGGNCCY 1 cut(s) 213
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 215, 1124
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 2 cut(s) 176, 573
ErhI CCWWGG 2 cut(s) 176, 573
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 8 cut(s) 295, 475, 600, 725, 799, 972, 1217, 1325
FalI AAGNNNNNCTT 2 cut(s) 784, 816
FaqI GGGAC 2 cut(s) 79, 1036
FatI CATG 8 cut(s) 291, 471, 596, 721, 795, 968, 1213, 1321
FauNDI CATATG 1 cut(s) 323
FblI GTMKAC 1 cut(s) 1104
Fnu4HI GCNGC 2 cut(s) 547, 1395
FokI GGATG 3 cut(s) 476, 1241, 1285
Fsp4HI GCNGC 2 cut(s) 547, 1395
GluI GCNGC 2 cut(s) 547, 1395
GsaI CCCAGC 1 cut(s) 1205
GsuI CTGGAG 1 cut(s) 430
HaeIII GGCC 6 cut(s) 85, 347, 420, 584, 919, 1006
HapII CCGG 3 cut(s) 348, 672, 864
Hin1II CATG 8 cut(s) 295, 475, 600, 725, 799, 972, 1217, 1325
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HindIII AAGCTT 4 cut(s) 100, 689, 1208, 1285
HinfI GANTC 8 cut(s) 127, 160, 398, 494, 632, 1169, 1175, 1346
HpaI GTTAAC 1 cut(s) 297
HpaII CCGG 3 cut(s) 348, 672, 864
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 4 cut(s) 47, 297, 1105, 1388
Hpy188I TCNGA 4 cut(s) 159, 455, 1174, 1351
Hpy188III TCNNGA 7 cut(s) 56, 140, 389, 956, 1082, 1157, 1363
Hpy8I GTNNAC 4 cut(s) 47, 297, 1105, 1388
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 146, 879, 1080, 1123
HpyCH4III ACNGT 4 cut(s) 51, 229, 1042, 1088
HpyCH4IV ACGT 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 7 cut(s) 364, 373, 836, 1012, 1016, 1207, 1394
HpyF3I CTNAG 3 cut(s) 587, 813, 1288
HpySE526I ACGT 1 cut(s) 64
Hsp92II CATG 8 cut(s) 295, 475, 600, 725, 799, 972, 1217, 1325
KspAI GTTAAC 1 cut(s) 297
Kzo9I GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
LmnI GCTCC 1 cut(s) 449
Lsp1109I GCAGC 2 cut(s) 533, 1381
LweI GCATC 6 cut(s) 80, 454, 581, 684, 1111, 1219
MaeII ACGT 1 cut(s) 64
MaeIII GTNAC 6 cut(s) 229, 382, 709, 819, 1036, 1157
MalI GATC 9 cut(s) 182, 339, 504, 762, 843, 954, 1068, 1097, 1359
MboI GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
MboII GAAGA 6 cut(s) 238, 363, 404, 731, 1154, 1337
MfeI CAATTG 2 cut(s) 648, 984
MflI RGATCY 3 cut(s) 180, 1066, 1357
MhlI GDGCHC 1 cut(s) 1390
MlsI TGGCCA 2 cut(s) 420, 919
MluCI AATT 9 cut(s) 171, 456, 648, 964, 984, 1052, 1152, 1242, 1371
MluNI TGGCCA 2 cut(s) 420, 919
MlyI GAGTC 1 cut(s) 1184
MmeI TCCRAC 2 cut(s) 682, 916
Mox20I TGGCCA 2 cut(s) 420, 919
MroXI GAANNNNTTC 2 cut(s) 871, 1146
MscI TGGCCA 2 cut(s) 420, 919
MseI TTAA 4 cut(s) 296, 413, 615, 680
MslI CAYNNNNRTG 1 cut(s) 1326
Msp20I TGGCCA 2 cut(s) 420, 919
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 3 cut(s) 348, 672, 864
MspR9I CCNGG 2 cut(s) 217, 1126
MunI CAATTG 2 cut(s) 648, 984
Mva1269I GAATGC 1 cut(s) 513
MvaI CCWGG 2 cut(s) 217, 1126
MwoI GCNNNNNNNGC 7 cut(s) 364, 373, 836, 1012, 1016, 1207, 1394
NdeI CATATG 1 cut(s) 323
NdeII GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
NlaIII CATG 8 cut(s) 295, 475, 600, 725, 799, 972, 1217, 1325
NlaIV GGNNCC 3 cut(s) 182, 445, 1068
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 1036, 1157
NspI RCATGY 2 cut(s) 295, 799
PaeI GCATGC 1 cut(s) 799
PciI ACATGT 1 cut(s) 291
PctI GAATGC 1 cut(s) 513
PdmI GAANNNNTTC 2 cut(s) 871, 1146
PfeI GAWTC 7 cut(s) 127, 160, 398, 494, 632, 1169, 1346
PkrI GCNGC 2 cut(s) 548, 1396
PleI GAGTC 1 cut(s) 1183
PpsI GAGTC 1 cut(s) 1183
Ppu21I YACGTR 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 213
PscI ACATGT 1 cut(s) 291
PshAI GACNNNNGTC 1 cut(s) 38
Psp5II RGGWCCY 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 215, 1124
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 2 cut(s) 215, 1124
PspN4I GGNNCC 3 cut(s) 182, 445, 1068
PspPI GGNCC 4 cut(s) 213, 304, 583, 1270
PspPPI RGGWCCY 1 cut(s) 213
PstI CTGCAG 1 cut(s) 1257
PsuI RGATCY 3 cut(s) 180, 1066, 1357
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 2 cut(s) 605, 911
RsaNI GTAC 2 cut(s) 604, 910
RseI CAYNNNNRTG 1 cut(s) 1326
SaqAI TTAA 4 cut(s) 296, 413, 615, 680
SatI GCNGC 2 cut(s) 547, 1395
Sau3AI GATC 9 cut(s) 180, 337, 502, 760, 841, 952, 1066, 1095, 1357
Sau96I GGNCC 4 cut(s) 213, 304, 583, 1270
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 2 cut(s) 217, 1126
SduI GDGCHC 1 cut(s) 1390
SfaNI GCATC 6 cut(s) 80, 454, 581, 684, 1111, 1219
SfcI CTRYAG 1 cut(s) 1253
SinI GGWCC 3 cut(s) 213, 304, 1270
SmiMI CAYNNNNRTG 1 cut(s) 1326
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
SnaBI TACGTA 1 cut(s) 65
SphI GCATGC 1 cut(s) 799
Sse9I AATT 9 cut(s) 171, 456, 648, 964, 984, 1052, 1152, 1242, 1371
SsiI CCGC 2 cut(s) 461, 601
StyD4I CCNGG 2 cut(s) 215, 1124
StyI CCWWGG 2 cut(s) 176, 573
TaaI ACNGT 4 cut(s) 51, 229, 1042, 1088
TaiI ACGT 1 cut(s) 67
TaqI TCGA 2 cut(s) 209, 505
TasI AATT 9 cut(s) 171, 456, 648, 964, 984, 1052, 1152, 1242, 1371
TatI WGTACW 1 cut(s) 909
TfiI GAWTC 7 cut(s) 127, 160, 398, 494, 632, 1169, 1346
Tru1I TTAA 4 cut(s) 296, 413, 615, 680
Tru9I TTAA 4 cut(s) 296, 413, 615, 680
TscAI CASTG 4 cut(s) 1068, 1093, 1171, 1269
TseFI GTSAC 2 cut(s) 1036, 1157
TseI GCWGC 2 cut(s) 546, 1394
Tsp45I GTSAC 2 cut(s) 1036, 1157
TspDTI ATGAA 8 cut(s) 206, 411, 821, 864, 957, 1202, 1287, 1338
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 4 cut(s) 1068, 1093, 1171, 1269
VneI GTGCAC 1 cut(s) 1386
VpaK11BI GGWCC 3 cut(s) 213, 304, 1270
XapI RAATTY 2 cut(s) 171, 964
XceI RCATGY 2 cut(s) 295, 799
XmaJI CCTAGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 2 cut(s) 871, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.