RLG00000001769

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012625
Physical Location & Seq
Forward (+)
20498774 .. 20500204
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001769

Sequence Viewer

Length: 1431 bp
ATGGGGGCTGTATTGAGTTTGAACAATTCTAGGACTAGGGAGGGTGGACCTCATGGGGTTTCACAGAGTGAAACACGCAAGAGGCAGAGGTTGTCTTCATGCCTTTATGAGGAAAACCCGCGATTGATTCCCAGTCTTCCTGACGAGATATCTATTCAGATTCTTGCCAGAATACCTAGGATCCACTATTTGAAATTGAAGATGGTGTCACGCACCTGGAAAACCACCGTTATGACTGCCGAAATTTTCAATTTGAGAAAAGAACTAGGAACAACAGAGGAATGGCTCTATGTATTAACAAAGGTTGAAAGTGATAAGCTCATATGGTATGCTCTGGACCCTCTGGCCGCAAAATGGCAGAGGTTGCCACCAATGCCTAGTGTTTGTTCTGAAGATGAATCTAGGAGGGCTTTAACTGCTCGTAGAATGTGGAAAATGGTAGGTTCAAGTATCAGACTTGCGGATGTCATCATGGGCTGGCTTGGTCGGAAGGATGCACTGGATCGAATGCCTTTTTGTGGTTGTGCCGTTGGTGCTGTTGATGGCTGCCTCTATGTCCTAGGTGGATTTTCAAGGGCTACAGCCCTATCATGTGCCTGGCGGTATAATCCAGTTATAAACTGTTGGAATGAATCAACTCCAATGTCAATGGGTAGAGCGTATTGTAAGACAGGCATTTTAAACAATAAACTTTATGTTGTTGGAGGGGTTACTAGAGGACGTGGTGGATTGGCCCCTCTTCAATCAGCTGAAGTATTTGATCCAAAAACAGGTATCTGGTCCCAAATACCAAGCATGCCCTTTGCCAAAGCTCAGGTGTTACCAACTGCATTTTTGGCTGATCTGCTCAAGCCTATTGCTACCGGGATGACTTCATACAAGGGGAGATTGTTTGTTCCACAAAGTTTATACTGCTGGCCGTTTTTTGTTGATGTTGGAGGTGAGGTTTATGATCCAGAGGTGAATTCATGGGGTGATATGCCAACTGGCATGGGGGAGGGTTGGCCTGCAAGGCAAGCAGGGACGAAAATGAGTGTCACAGTCGATGGTGAATTGTATGCACTGGATCCTTCTAGTTCTCTTGACAATGCCAAGATCAAGTTATATGATTACCAAGATGATGCTTGGAATGTTGTTGCAGAAGATGTTCCAATTCGGGACTTTACTGAATCAGAGTCTCCCTATTTACTTGCTGGTTTCCTTGGGAAGCTTCATGTGATCACGAAGGATGCCAATAACAATGTTGCAGTTCTACAGGCTTATGTGCCGAACCGTCATGCTTCCACCTCAGAAGCTTCATCCTCATCACCAGATGATACCTCTGAAATTTCTGAACCACAAGCAGAGTCAGAAGTAGATCTTTGGAAGGTAATTGCCACAAGGAGTTCACGGACTGCTGAACTGGTTAGCTGTCTCGTCCTTGATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

477

Amino Acids

52.73

Weight (kDa)

6.2

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 79 5.6e-07 F-box domain
Kelch_FKB95 PF25210 168 - 407 8.6e-13 FKB95, Kelch-repeats domain
Kelch_1 PF01344 171 - 216 9.6e-13 Kelch motif
Beta-prop_Calicin PF13964 176 - 268 2.7e-08 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 176 - 266 3.6e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 219 - 267 3.5e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 617
AccII CGCG 1 cut(s) 121
AciI CCGC 4 cut(s) 119, 348, 461, 601
AclWI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
AcoI YGGCCR 2 cut(s) 345, 917
AcsI RAATTY 3 cut(s) 243, 964, 1326
AcuI CTGAAG 2 cut(s) 411, 771
AdeI CACNNNGTG 1 cut(s) 68
AfiI CCNNNNNNNGG 4 cut(s) 109, 354, 518, 770
AgsI TTSAA 8 cut(s) 22, 193, 199, 250, 308, 447, 573, 743
AjiI CACGTC 1 cut(s) 722
AjnI CCWGG 2 cut(s) 215, 596
AluBI AGCT 6 cut(s) 319, 749, 812, 1210, 1295, 1410
AluI AGCT 6 cut(s) 319, 749, 812, 1210, 1295, 1410
Alw26I GTCTC 2 cut(s) 1182, 1418
AlwI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
AoxI GGCC 4 cut(s) 345, 732, 917, 1004
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 3 cut(s) 243, 964, 1326
Asp700I GAANNNNTTC 1 cut(s) 1146
AspA2I CCTAGG 2 cut(s) 176, 559
AspS9I GGNCC 4 cut(s) 47, 337, 733, 780
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 5 cut(s) 953, 973, 986, 1061, 1299
AvaII GGWCC 3 cut(s) 47, 337, 780
AvrII CCTAGG 2 cut(s) 176, 559
BamHI GGATCC 2 cut(s) 180, 1066
BbsI GAAGAC 2 cut(s) 87, 128
BbvI GCAGC 1 cut(s) 533
BccI CCATC 3 cut(s) 196, 536, 1040
BceAI ACGGC 2 cut(s) 512, 904
BciT130I CCWGG 2 cut(s) 217, 598
BclI TGATCA 1 cut(s) 1218
BcnI CCSGG 1 cut(s) 865
BcoDI GTCTC 2 cut(s) 1182, 1418
BfaI CTAG 9 cut(s) 30, 36, 177, 266, 378, 402, 560, 714, 1074
BfmI CTRYAG 2 cut(s) 579, 1253
BglI GCCNNNNNGGC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 1357
BisI GCNGC 2 cut(s) 348, 547
BlnI CCTAGG 2 cut(s) 176, 559
BlsI GCNGC 2 cut(s) 349, 548
Bme1390I CCNGG 3 cut(s) 217, 598, 865
Bme18I GGWCC 3 cut(s) 47, 337, 780
BmgBI CACGTC 1 cut(s) 722
BmgT120I GGNCC 4 cut(s) 47, 337, 733, 780
BmiI GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
BmrFI CCNGG 3 cut(s) 217, 598, 865
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 3 cut(s) 484, 1111, 1219
BmuI ACTGGG 1 cut(s) 126
BpiI GAAGAC 2 cut(s) 87, 128
Bpu10I CCTNAGC 1 cut(s) 813
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 865
BsaJI CCNNGG 3 cut(s) 176, 559, 1201
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 354, 518, 770
Bse1I ACTGG 6 cut(s) 132, 504, 611, 991, 1068, 1407
BseBI CCWGG 2 cut(s) 217, 598
BseDI CCNNGG 3 cut(s) 176, 559, 1201
BseGI GGATG 5 cut(s) 469, 499, 873, 1234, 1298
BseLI CCNNNNNNNGG 4 cut(s) 109, 354, 518, 770
BseMII CTCAG 2 cut(s) 827, 1302
BseNI ACTGG 6 cut(s) 132, 504, 611, 991, 1068, 1407
BseXI GCAGC 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 4 cut(s) 347, 734, 919, 1006
BsiSI CCGG 1 cut(s) 864
BslFI GGGAC 3 cut(s) 766, 1036, 1172
BslI CCNNNNNNNGG 4 cut(s) 109, 354, 518, 770
BsmAI GTCTC 2 cut(s) 1182, 1418
BsmFI GGGAC 3 cut(s) 766, 1036, 1172
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 4 cut(s) 347, 734, 919, 1006
BspACI CCGC 4 cut(s) 119, 348, 461, 601
BspANI GGCC 4 cut(s) 347, 734, 919, 1006
BspCNI CTCAG 2 cut(s) 826, 1301
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
BspPI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
BsrI ACTGG 6 cut(s) 132, 504, 611, 991, 1068, 1407
BssECI CCNNGG 3 cut(s) 176, 559, 1201
BssT1I CCWWGG 3 cut(s) 176, 559, 1201
Bst2UI CCWGG 2 cut(s) 217, 598
Bst4CI ACNGT 4 cut(s) 229, 623, 1042, 1274
Bst6I CTCTTC 1 cut(s) 744
BstAPI GCANNNNNTGC 1 cut(s) 364
BstC8I GCNNGC 5 cut(s) 479, 797, 917, 1008, 1017
BstDEI CTNAG 2 cut(s) 813, 1288
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 5 cut(s) 469, 499, 873, 1234, 1298
BstFNI CGCG 1 cut(s) 121
BstMAI GTCTC 2 cut(s) 1182, 1418
BstMWI GCNNNNNNNGC 7 cut(s) 364, 373, 416, 533, 836, 1012, 1016
BstNI CCWGG 2 cut(s) 217, 598
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 3 cut(s) 215, 596, 863
BstSFI CTRYAG 2 cut(s) 579, 1253
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 2 cut(s) 87, 128
BstX2I RGATCY 3 cut(s) 180, 1066, 1357
BstYI RGATCY 3 cut(s) 180, 1066, 1357
BsuRI GGCC 4 cut(s) 347, 734, 919, 1006
BtrI CACGTC 1 cut(s) 722
BtsCI GGATG 5 cut(s) 469, 499, 873, 1234, 1298
BtsIMutI CAGTG 2 cut(s) 497, 1061
Cac8I GCNNGC 5 cut(s) 479, 797, 917, 1008, 1017
Cfr13I GGNCC 4 cut(s) 47, 337, 733, 780
CviAII CATG 9 cut(s) 53, 99, 472, 591, 796, 969, 991, 1214, 1277
DdeI CTNAG 2 cut(s) 813, 1288
DraI TTTAAA 1 cut(s) 681
DraIII CACNNNGTG 1 cut(s) 68
EaeI YGGCCR 2 cut(s) 345, 917
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco130I CCWWGG 3 cut(s) 176, 559, 1201
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 3 cut(s) 47, 337, 780
Eco57I CTGAAG 2 cut(s) 411, 771
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 2 cut(s) 215, 596
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 3 cut(s) 176, 559, 1201
ErhI CCWWGG 3 cut(s) 176, 559, 1201
FaeI CATG 9 cut(s) 56, 102, 475, 594, 799, 972, 994, 1217, 1280
FalI AAGNNNNNCTT 2 cut(s) 1344, 1376
FaqI GGGAC 3 cut(s) 766, 1036, 1172
FatI CATG 9 cut(s) 52, 98, 471, 590, 795, 968, 990, 1213, 1276
FauI CCCGC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 323
FbaI TGATCA 1 cut(s) 1218
Fnu4HI GCNGC 2 cut(s) 348, 547
FokI GGATG 5 cut(s) 476, 506, 880, 1241, 1285
Fsp4HI GCNGC 2 cut(s) 348, 547
FspBI CTAG 9 cut(s) 30, 36, 177, 266, 378, 402, 560, 714, 1074
GluI GCNGC 2 cut(s) 348, 547
HaeIII GGCC 4 cut(s) 347, 734, 919, 1006
HapII CCGG 1 cut(s) 864
Hin1II CATG 9 cut(s) 56, 102, 475, 594, 799, 972, 994, 1217, 1280
HindIII AAGCTT 2 cut(s) 1208, 1293
HinfI GANTC 7 cut(s) 127, 160, 398, 632, 1169, 1175, 1346
HpaII CCGG 1 cut(s) 864
HphI GGTGA 5 cut(s) 953, 973, 986, 1061, 1299
Hpy166II GTNNAC 2 cut(s) 47, 1388
Hpy188I TCNGA 9 cut(s) 159, 391, 455, 489, 1174, 1291, 1324, 1333, 1351
Hpy188III TCNNGA 6 cut(s) 140, 335, 956, 1082, 1157, 1222
Hpy8I GTNNAC 2 cut(s) 47, 1388
HpyAV CCTTC 4 cut(s) 484, 1080, 1219, 1360
HpyCH4III ACNGT 4 cut(s) 229, 623, 1042, 1274
HpyCH4IV ACGT 1 cut(s) 721
HpyCH4V TGCA 6 cut(s) 497, 830, 1010, 1061, 1139, 1247
HpyF10VI GCNNNNNNNGC 7 cut(s) 364, 373, 416, 533, 836, 1012, 1016
HpyF3I CTNAG 2 cut(s) 813, 1288
HpySE526I ACGT 1 cut(s) 721
Hsp92II CATG 9 cut(s) 56, 102, 475, 594, 799, 972, 994, 1217, 1280
Ksp22I TGATCA 1 cut(s) 1218
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 3 cut(s) 484, 1111, 1219
MaeI CTAG 9 cut(s) 30, 36, 177, 266, 378, 402, 560, 714, 1074
MaeII ACGT 1 cut(s) 721
MaeIII GTNAC 4 cut(s) 207, 709, 819, 1036
MboII GAAGA 6 cut(s) 87, 128, 211, 404, 731, 1154
MflI RGATCY 3 cut(s) 180, 1066, 1357
MluCI AATT 9 cut(s) 25, 194, 243, 250, 964, 1052, 1152, 1326, 1371
MlyI GAGTC 2 cut(s) 1184, 1355
MmeI TCCRAC 4 cut(s) 467, 605, 682, 916
MroXI GAANNNNTTC 1 cut(s) 1146
MseI TTAA 3 cut(s) 296, 413, 680
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 1 cut(s) 864
MspR9I CCNGG 3 cut(s) 217, 598, 865
Mva1269I GAATGC 1 cut(s) 513
MvaI CCWGG 2 cut(s) 217, 598
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 7 cut(s) 364, 373, 416, 533, 836, 1012, 1016
NciI CCSGG 1 cut(s) 865
NdeI CATATG 1 cut(s) 323
NlaIII CATG 9 cut(s) 56, 102, 475, 594, 799, 972, 994, 1217, 1280
NlaIV GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
NmuCI GTSAC 2 cut(s) 207, 1036
NspI RCATGY 1 cut(s) 799
PaeI GCATGC 1 cut(s) 799
PctI GAATGC 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 1146
PfeI GAWTC 5 cut(s) 127, 160, 398, 632, 1169
PkrI GCNGC 2 cut(s) 349, 548
PleI GAGTC 2 cut(s) 1183, 1354
PpsI GAGTC 2 cut(s) 1183, 1354
PsiI TTATAA 1 cut(s) 617
Psp6I CCWGG 2 cut(s) 215, 596
PspGI CCWGG 2 cut(s) 215, 596
PspN4I GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
PspPI GGNCC 4 cut(s) 47, 337, 733, 780
PsuI RGATCY 3 cut(s) 180, 1066, 1357
PvuII CAGCTG 1 cut(s) 749
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 3 cut(s) 296, 413, 680
SatI GCNGC 2 cut(s) 348, 547
Sau96I GGNCC 4 cut(s) 47, 337, 733, 780
SchI GAGTC 2 cut(s) 1184, 1355
ScrFI CCNGG 3 cut(s) 217, 598, 865
SfaNI GCATC 3 cut(s) 484, 1111, 1219
SfcI CTRYAG 2 cut(s) 579, 1253
SinI GGWCC 3 cut(s) 47, 337, 780
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 848
SmoI CTYRAG 1 cut(s) 848
SphI GCATGC 1 cut(s) 799
Sse9I AATT 9 cut(s) 25, 194, 243, 250, 964, 1052, 1152, 1326, 1371
SsiI CCGC 4 cut(s) 119, 348, 461, 601
SspMI CTAG 9 cut(s) 30, 36, 177, 266, 378, 402, 560, 714, 1074
StyD4I CCNGG 3 cut(s) 215, 596, 863
StyI CCWWGG 3 cut(s) 176, 559, 1201
TaaI ACNGT 4 cut(s) 229, 623, 1042, 1274
TaiI ACGT 1 cut(s) 724
TaqI TCGA 2 cut(s) 505, 1044
TasI AATT 9 cut(s) 25, 194, 243, 250, 964, 1052, 1152, 1326, 1371
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 5 cut(s) 127, 160, 398, 632, 1169
Tru1I TTAA 3 cut(s) 296, 413, 680
Tru9I TTAA 3 cut(s) 296, 413, 680
TscAI CASTG 2 cut(s) 504, 1068
TseFI GTSAC 2 cut(s) 207, 1036
TseI GCWGC 1 cut(s) 546
Tsp45I GTSAC 2 cut(s) 207, 1036
TspDTI ATGAA 7 cut(s) 87, 411, 645, 864, 957, 1202, 1287
TspGWI ACGGA 1 cut(s) 1405
TspRI CASTG 2 cut(s) 504, 1068
VpaK11BI GGWCC 3 cut(s) 47, 337, 780
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 3 cut(s) 243, 964, 1326
XceI RCATGY 1 cut(s) 799
XmaJI CCTAGG 2 cut(s) 176, 559
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 9 cut(s) 30, 36, 177, 266, 378, 402, 560, 714, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.