MD15G1412100.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012625
Physical Location & Seq
Forward (+)
51167876 .. 51170450
2575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1412100.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGGGGGCTATATTGAGCTTGAATCATTCCAGGACTAGGGTCAGTGAACCTTCTCAAGATTTGCTGAGTGCATCATGCAAGAGGATGAAGTTGTCTCCAAGCTTTTATGACGAAAACCCAAGATTGATTCCCTGCATTCCTGACGAGATATCTATTCAGATTCTTGCCAGAATTCCTAGGATCCACTATTTGAAGCTCAAGTCTGTTTCACGGACCTGGAAAGCCACCATTACGAGTCCTGAACTTTTTTCTTTGAGAAAAGAACTAGGAACAACGGAGGAATGGCTCTACATGTTAACAAAGGTCCAAAGTGATAAGCTCGTATGGTGTGCTCTGGATCCTCTGGCCAGAAAATGGCAAAGGTTGCCATCTATGCCTAATGTTACTCCGGAAGAAGAATCTAGGAAGGGTTTAACTGGCCAGAGAATGTGGAACATGGCAGGCTCTAGTGTCAGAATTGCAGATGTCATCATGGGTTGGCTTGGGAGGAAGGATTCACTGGATCGAATGCCTTTTTGTGGTTGCTCTATTGGGGCTATTGATGGCTGCCTCTATGTGCTAGGGGGATTTTCAAGGGCTTCGGCTTTGAGATGCACATGGCGGTACAATCCAGTTAAAAATACTTGGAGTGAATCAAGTCCAATGTCTATTGGTAGAGCTTATTGTAAGACCGGCATCTTAAACGATAAGCTTTATGTTGTTGGAGGTGTTACAAGGGGTCACGGTGGATTGACCCCTCTTCAATCAGCTGAAGTATTTGATCCAAAAACAGGTGTGTGGTCCCAATTACCAAGCATGCCTTTTACGAAAGCTCAGGTGTTACCAACTGCATTTTTGGCTGACCTGCTGAAGCCTATTGCAACCGGAATGACTTCATACAGGGGAAGGTTGTTCGTTCCACAGAGTTTGTACTGCTGGCCATTTTTTGTTGACGTTGGAGGAGAGGTTTATGATCCAGAGGCAAATTCATGGGTTGAAATGCCAACTGGCATGGGAGAGGGTTGGCCTGCAAGGCAGGCAGGGACAAAAATGAGTGTCACAGTTGATGGCGAATTGTATGCATTGGATCCTTCTAGTTCTCTTGACAGTGCAAAGATCAAGGTATACGATTACCAAGCTGATGCCTGGAAAGTTGTTGCAGAAGATGTTCCAATTCGTGACTTCACTGAATCAGAGTCTCCCTATTTGCTTGCTGGTTTGCTGGGGAAGCTTCATGTGATCACAAGGGATGCAAATAACAACATTGCTGTTCTGCAGGCTTCTGTGCCGGACCATTTTGCATCGATCTCAGTAGCTTCATCCTCATCTCGCAATGACCCCCATGAAGATGTTGAACGACCAGCAGAATCAGAACGAGATCCTTGGAAAGTAATTGCCACAAGGAGTGCACGGGCTGCTGAACTGGTTAGCTGTCTAGTCCTTGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

477

Amino Acids

52.75

Weight (kDa)

7.51

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 83 9.7e-08 F-box domain
Kelch_FKB95 PF25210 166 - 388 3.9e-14 FKB95, Kelch-repeats domain
Kelch_1 PF01344 171 - 216 7.1e-13 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 174 - 266 6e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 176 - 268 9e-07 Calicin, beta-propeller domain
Kelch_1 PF01344 219 - 267 1.3e-12 Kelch motif
Kelch_2 PF07646 219 - 267 9e-06 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 852
AccB7I CCANNNNNTGG 1 cut(s) 354
AccI GTMKAC 1 cut(s) 1104
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 1 cut(s) 601
AcoI YGGCCR 3 cut(s) 345, 418, 917
AcsI RAATTY 2 cut(s) 171, 964
AcuI CTGAAG 2 cut(s) 771, 869
AfaI GTAC 2 cut(s) 605, 911
AfiI CCNNNNNNNGG 4 cut(s) 36, 354, 518, 770
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 6 cut(s) 22, 193, 573, 743, 977, 1334
AjnI CCWGG 3 cut(s) 29, 215, 1124
Alw21I GWGCWC 2 cut(s) 334, 1390
Alw26I GTCTC 2 cut(s) 99, 1182
Alw44I GTGCAC 1 cut(s) 1386
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 4 cut(s) 345, 418, 917, 1004
ApaLI GTGCAC 1 cut(s) 1386
ApeKI GCWGC 2 cut(s) 546, 1394
ApoI RAATTY 2 cut(s) 171, 964
Asp700I GAANNNNTTC 2 cut(s) 871, 1146
AspA2I CCTAGG 1 cut(s) 176
AspS9I GGNCC 4 cut(s) 213, 304, 780, 1270
AvaII GGWCC 4 cut(s) 213, 304, 780, 1270
AvrII CCTAGG 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 1390
BalI TGGCCA 3 cut(s) 347, 420, 919
BamHI GGATCC 3 cut(s) 180, 337, 1066
Bbv12I GWGCWC 2 cut(s) 334, 1390
BbvI GCAGC 2 cut(s) 533, 1381
BccI CCATC 3 cut(s) 376, 536, 1040
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 3 cut(s) 31, 217, 1126
BclI TGATCA 1 cut(s) 1218
BcoDI GTCTC 2 cut(s) 99, 1182
BfaI CTAG 8 cut(s) 36, 177, 266, 402, 447, 560, 1074, 1415
BfmI CTRYAG 1 cut(s) 1253
BfuAI ACCTGC 1 cut(s) 852
BglI GCCNNNNNGGC 1 cut(s) 1012
BisI GCNGC 2 cut(s) 547, 1395
BlnI CCTAGG 1 cut(s) 176
BlsI GCNGC 2 cut(s) 548, 1396
Bme1390I CCNGG 3 cut(s) 31, 217, 1126
Bme18I GGWCC 4 cut(s) 213, 304, 780, 1270
BmgT120I GGNCC 4 cut(s) 213, 304, 780, 1270
BmiI GGNNCC 4 cut(s) 182, 339, 782, 1068
BmrFI CCNGG 3 cut(s) 31, 217, 1126
BmsI GCATC 6 cut(s) 80, 581, 684, 1111, 1219, 1289
BoxI GACNNNNGTC 1 cut(s) 38
Bpu10I CCTNAGC 1 cut(s) 813
BpuEI CTTGAG 2 cut(s) 39, 182
Bsa29I ATCGAT 1 cut(s) 1283
BsaJI CCNNGG 2 cut(s) 176, 1361
BsaWI WCCGGW 2 cut(s) 388, 863
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 354, 518, 770
Bse118I RCCGGY 1 cut(s) 671
Bse1I ACTGG 5 cut(s) 421, 504, 611, 991, 1407
Bse3DI GCAATG 2 cut(s) 1242, 1318
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 3 cut(s) 31, 217, 1126
BseCI ATCGAT 1 cut(s) 1283
BseDI CCNNGG 2 cut(s) 176, 1361
BseGI GGATG 3 cut(s) 90, 1234, 1298
BseLI CCNNNNNNNGG 4 cut(s) 36, 354, 518, 770
BseMI GCAATG 2 cut(s) 1242, 1318
BseMII CTCAG 3 cut(s) 56, 827, 1302
BseNI ACTGG 5 cut(s) 421, 504, 611, 991, 1407
BseRI GAGGAG 1 cut(s) 954
BseSI GKGCMC 1 cut(s) 1390
BseXI GCAGC 2 cut(s) 533, 1381
BseYI CCCAGC 1 cut(s) 1201
BshFI GGCC 4 cut(s) 347, 420, 919, 1006
BshVI ATCGAT 1 cut(s) 1283
BsiHKAI GWGCWC 2 cut(s) 334, 1390
BsiSI CCGG 4 cut(s) 389, 672, 864, 1268
BslFI GGGAC 2 cut(s) 766, 1036
BslI CCNNNNNNNGG 4 cut(s) 36, 354, 518, 770
BsmAI GTCTC 2 cut(s) 99, 1182
BsmFI GGGAC 2 cut(s) 766, 1036
BsmI GAATGC 2 cut(s) 135, 513
BsnI GGCC 4 cut(s) 347, 420, 919, 1006
Bsp1286I GDGCHC 2 cut(s) 334, 1390
Bsp13I TCCGGA 1 cut(s) 388
BspACI CCGC 1 cut(s) 601
BspANI GGCC 4 cut(s) 347, 420, 919, 1006
BspCNI CTCAG 3 cut(s) 57, 826, 1301
BspDI ATCGAT 1 cut(s) 1283
BspEI TCCGGA 1 cut(s) 388
BspLI GGNNCC 4 cut(s) 182, 339, 782, 1068
BspMAI CTGCAG 1 cut(s) 1257
BspMI ACCTGC 1 cut(s) 852
BsrDI GCAATG 2 cut(s) 1242, 1318
BsrFI RCCGGY 1 cut(s) 671
BsrI ACTGG 5 cut(s) 421, 504, 611, 991, 1407
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 2 cut(s) 176, 1361
BssNAI GTATAC 1 cut(s) 1105
BssT1I CCWWGG 2 cut(s) 176, 1361
Bst1107I GTATAC 1 cut(s) 1105
Bst2UI CCWGG 3 cut(s) 31, 217, 1126
Bst4CI ACNGT 3 cut(s) 725, 1042, 1088
Bst6I CTCTTC 1 cut(s) 744
BstAPI GCANNNNNTGC 2 cut(s) 364, 1394
BstC8I GCNNGC 7 cut(s) 442, 797, 917, 1008, 1017, 1191, 1257
BstDEI CTNAG 3 cut(s) 65, 813, 1288
BstF5I GGATG 3 cut(s) 90, 1234, 1298
BstMAI GTCTC 2 cut(s) 99, 1182
BstMWI GCNNNNNNNGC 7 cut(s) 364, 373, 836, 1012, 1016, 1207, 1394
BstNI CCWGG 3 cut(s) 31, 217, 1126
BstNSI RCATGY 2 cut(s) 295, 799
BstPAI GACNNNNGTC 1 cut(s) 38
BstSCI CCNGG 3 cut(s) 29, 215, 1124
BstSFI CTRYAG 1 cut(s) 1253
BstSLI GKGCMC 1 cut(s) 1390
BstV1I GCAGC 2 cut(s) 533, 1381
BstX2I RGATCY 4 cut(s) 180, 337, 1066, 1357
BstYI RGATCY 4 cut(s) 180, 337, 1066, 1357
BstZ17I GTATAC 1 cut(s) 1105
Bsu15I ATCGAT 1 cut(s) 1283
BsuRI GGCC 4 cut(s) 347, 420, 919, 1006
BsuTUI ATCGAT 1 cut(s) 1283
BtsCI GGATG 3 cut(s) 90, 1234, 1298
BtsIMutI CAGTG 4 cut(s) 49, 497, 1093, 1164
BveI ACCTGC 1 cut(s) 852
Cac8I GCNNGC 7 cut(s) 442, 797, 917, 1008, 1017, 1191, 1257
Cfr10I RCCGGY 1 cut(s) 671
Cfr13I GGNCC 4 cut(s) 213, 304, 780, 1270
ClaI ATCGAT 1 cut(s) 1283
Csp6I GTAC 2 cut(s) 604, 910
CspCI CAANNNNNGTGG 2 cut(s) 888, 923
CviQI GTAC 2 cut(s) 604, 910
DdeI CTNAG 3 cut(s) 65, 813, 1288
EaeI YGGCCR 3 cut(s) 345, 418, 917
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco130I CCWWGG 2 cut(s) 176, 1361
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 4 cut(s) 213, 304, 780, 1270
Eco57I CTGAAG 2 cut(s) 771, 869
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 3 cut(s) 29, 215, 1124
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 2 cut(s) 176, 1361
EcoT22I ATGCAT 1 cut(s) 1063
ErhI CCWWGG 2 cut(s) 176, 1361
FalI AAGNNNNNCTT 2 cut(s) 784, 816
FaqI GGGAC 2 cut(s) 766, 1036
FbaI TGATCA 1 cut(s) 1218
FblI GTMKAC 1 cut(s) 1104
Fnu4HI GCNGC 2 cut(s) 547, 1395
FokI GGATG 3 cut(s) 97, 1241, 1285
Fsp4HI GCNGC 2 cut(s) 547, 1395
FspBI CTAG 8 cut(s) 36, 177, 266, 402, 447, 560, 1074, 1415
GluI GCNGC 2 cut(s) 547, 1395
GsaI CCCAGC 1 cut(s) 1205
HaeIII GGCC 4 cut(s) 347, 420, 919, 1006
HapII CCGG 4 cut(s) 389, 672, 864, 1268
HincII GTYRAC 2 cut(s) 297, 931
HindII GTYRAC 2 cut(s) 297, 931
HindIII AAGCTT 3 cut(s) 100, 689, 1208
HpaI GTTAAC 1 cut(s) 297
HpaII CCGG 4 cut(s) 389, 672, 864, 1268
Hpy166II GTNNAC 5 cut(s) 47, 297, 931, 1105, 1388
Hpy188I TCNGA 4 cut(s) 159, 455, 1174, 1351
Hpy188III TCNNGA 8 cut(s) 56, 140, 239, 335, 389, 956, 1082, 1157
Hpy8I GTNNAC 5 cut(s) 47, 297, 931, 1105, 1388
HpyAV CCTTC 5 cut(s) 60, 400, 484, 879, 1080
HpyCH4III ACNGT 3 cut(s) 725, 1042, 1088
HpyCH4IV ACGT 1 cut(s) 933
HpyF10VI GCNNNNNNNGC 7 cut(s) 364, 373, 836, 1012, 1016, 1207, 1394
HpyF3I CTNAG 3 cut(s) 65, 813, 1288
HpySE526I ACGT 1 cut(s) 933
Kpn2I TCCGGA 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 1218
KspAI GTTAAC 1 cut(s) 297
Lsp1109I GCAGC 2 cut(s) 533, 1381
LweI GCATC 6 cut(s) 80, 581, 684, 1111, 1219, 1289
MaeI CTAG 8 cut(s) 36, 177, 266, 402, 447, 560, 1074, 1415
MaeII ACGT 1 cut(s) 933
MaeIII GTNAC 6 cut(s) 382, 709, 719, 819, 1036, 1157
MboII GAAGA 5 cut(s) 404, 407, 731, 1154, 1337
MflI RGATCY 4 cut(s) 180, 337, 1066, 1357
MhlI GDGCHC 2 cut(s) 334, 1390
MlsI TGGCCA 3 cut(s) 347, 420, 919
MluCI AATT 7 cut(s) 171, 456, 785, 964, 1052, 1152, 1371
MluNI TGGCCA 3 cut(s) 347, 420, 919
MlyI GAGTC 2 cut(s) 244, 1184
MmeI TCCRAC 2 cut(s) 682, 916
Mox20I TGGCCA 3 cut(s) 347, 420, 919
Mph1103I ATGCAT 1 cut(s) 1063
MroI TCCGGA 1 cut(s) 388
MroXI GAANNNNTTC 2 cut(s) 871, 1146
MscI TGGCCA 3 cut(s) 347, 420, 919
MseI TTAA 4 cut(s) 296, 413, 615, 680
MslI CAYNNNNRTG 1 cut(s) 1326
Msp20I TGGCCA 3 cut(s) 347, 420, 919
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 4 cut(s) 389, 672, 864, 1268
MspR9I CCNGG 3 cut(s) 31, 217, 1126
Mva1269I GAATGC 2 cut(s) 135, 513
MvaI CCWGG 3 cut(s) 31, 217, 1126
MwoI GCNNNNNNNGC 7 cut(s) 364, 373, 836, 1012, 1016, 1207, 1394
NlaIV GGNNCC 4 cut(s) 182, 339, 782, 1068
NmuCI GTSAC 3 cut(s) 719, 1036, 1157
NsiI ATGCAT 1 cut(s) 1063
NspI RCATGY 2 cut(s) 295, 799
PaeI GCATGC 1 cut(s) 799
PciI ACATGT 1 cut(s) 291
PctI GAATGC 2 cut(s) 135, 513
PdmI GAANNNNTTC 2 cut(s) 871, 1146
PfeI GAWTC 8 cut(s) 22, 127, 160, 398, 494, 632, 1169, 1346
PflMI CCANNNNNTGG 1 cut(s) 354
PfoI TCCNGGA 1 cut(s) 29
PkrI GCNGC 2 cut(s) 548, 1396
PleI GAGTC 2 cut(s) 243, 1183
PpsI GAGTC 2 cut(s) 243, 1183
PscI ACATGT 1 cut(s) 291
PshAI GACNNNNGTC 1 cut(s) 38
Psp6I CCWGG 3 cut(s) 29, 215, 1124
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 3 cut(s) 29, 215, 1124
PspN4I GGNNCC 4 cut(s) 182, 339, 782, 1068
PspPI GGNCC 4 cut(s) 213, 304, 780, 1270
PstI CTGCAG 1 cut(s) 1257
PsuI RGATCY 4 cut(s) 180, 337, 1066, 1357
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 2 cut(s) 605, 911
RsaNI GTAC 2 cut(s) 604, 910
RseI CAYNNNNRTG 1 cut(s) 1326
SaqAI TTAA 4 cut(s) 296, 413, 615, 680
SatI GCNGC 2 cut(s) 547, 1395
Sau96I GGNCC 4 cut(s) 213, 304, 780, 1270
SchI GAGTC 2 cut(s) 244, 1184
ScrFI CCNGG 3 cut(s) 31, 217, 1126
SduI GDGCHC 2 cut(s) 334, 1390
SfaNI GCATC 6 cut(s) 80, 581, 684, 1111, 1219, 1289
SfcI CTRYAG 1 cut(s) 1253
SinI GGWCC 4 cut(s) 213, 304, 780, 1270
SmiMI CAYNNNNRTG 1 cut(s) 1326
SmlI CTYRAG 2 cut(s) 54, 197
SmoI CTYRAG 2 cut(s) 54, 197
SphI GCATGC 1 cut(s) 799
Sse9I AATT 7 cut(s) 171, 456, 785, 964, 1052, 1152, 1371
SsiI CCGC 1 cut(s) 601
SspMI CTAG 8 cut(s) 36, 177, 266, 402, 447, 560, 1074, 1415
StyD4I CCNGG 3 cut(s) 29, 215, 1124
StyI CCWWGG 2 cut(s) 176, 1361
TaaI ACNGT 3 cut(s) 725, 1042, 1088
TaiI ACGT 1 cut(s) 936
TaqI TCGA 2 cut(s) 505, 1283
TasI AATT 7 cut(s) 171, 456, 785, 964, 1052, 1152, 1371
TatI WGTACW 1 cut(s) 909
TfiI GAWTC 8 cut(s) 22, 127, 160, 398, 494, 632, 1169, 1346
Tru1I TTAA 4 cut(s) 296, 413, 615, 680
Tru9I TTAA 4 cut(s) 296, 413, 615, 680
TscAI CASTG 4 cut(s) 49, 504, 1093, 1171
TseFI GTSAC 3 cut(s) 719, 1036, 1157
TseI GCWGC 2 cut(s) 546, 1394
Tsp45I GTSAC 3 cut(s) 719, 1036, 1157
TspDTI ATGAA 6 cut(s) 101, 864, 957, 1202, 1287, 1338
TspGWI ACGGA 2 cut(s) 226, 290
TspRI CASTG 4 cut(s) 49, 504, 1093, 1171
Van91I CCANNNNNTGG 1 cut(s) 354
VneI GTGCAC 1 cut(s) 1386
VpaK11BI GGWCC 4 cut(s) 213, 304, 780, 1270
XapI RAATTY 2 cut(s) 171, 964
XceI RCATGY 2 cut(s) 295, 799
XmaJI CCTAGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 2 cut(s) 871, 1146
XspI CTAG 8 cut(s) 36, 177, 266, 402, 447, 560, 1074, 1415
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.