RchiOBHm_Chr7g0226051

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012625
Physical Location & Seq
Reverse (-)
49102697 .. 49105387
2691 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20246

Sequence Viewer

Length: 1431 bp
ATGGGGGCTATATTGAGTTTGAACAATTGTAGGACTAGGGAGAGTGGACCTCATGGGGTTTCACAGAGTGAAACACGCAAGAGGCAGAGGTTGTCTTCATGCCTTTATGAGGAAAACCCGAGATTGATTCCCAGTCTTCCTGACGAGATATCTATTCAGATTCTTGCCAGAATACCTAGGATCCACTATTTGAAATTGAAGATGGTGTCACGTACATGGAAAACCACCGTTATGACCGCCGAAATTTTCAATTTGAGAAAAGAACTAGGAACAACAGAGGAATGGCTCTATGTATTAACAAAGGTTGAAAGTGATAAGCTCATATGGTATGCTCTGGACCCTCTGGCAGCAAAATGGCAAAGGTTGCCACCAATGCCTAGTGTTTGTCCTGAAGATGAATCTAGGAGGGCTTTAACTGCTCGTAGAATGTGGAAAATGGTAGGTTCAAGTATCAGACTTGCGGATGTCATCATGGGCTGGCTTGGTCGGAAGGATGCACTGGATCGAATGCCTTTTTGTGGTTGTGCCGTTGGTGCTGTTGATGGCTGCCTCTATGTCCTAGGTGGATTTTCAAGGGCTACAGCCCTATCATGTGCCTGGCGGTATAATCCAGTTATAAACTCTTGGAGTGAATCAACTCCAATGTCAATGGGTAGAGCGTATTGTAAGACAGGCATTTTAAACAATAAACTTTATGTTGTTGGAGGGGTTACTAGAGGACGTGGTGGATTGGCCCCTCTTCAATCAGCTGAAGTATTTGATCCAAAAACAGGTATATGGTCCCAAATACCAAGCATGCCCTTTGCCAAAGCTCAGGTGTTACCAACTGCATTTTTGGCTGATCTGCTCAAGCCTATTGCTACCGGGATGACTTCATACAAGGGGAGATTGTTTGTTCCACAAAGTTTATACTGCTGGCCGTTTTTTGTTGATGTTGGAGGTGAGGTTTATGATCCAGAGGTGAATTCATGGGGTGATATGCCAACTGGCATGGGGGAGGGTTGGCCTGCAAGGCAAGCAGGGACGAAAATGAGTGTCACAGTCGATGGTGAATTGTATGCACTGGATCCATCTAGTTCTCTTGACAATGCCAAGATCAAGTTATATGATTACCAAGATGATGCTTGGAATGTTGTTGCAGAAGATGTTCCAATTCGGGACTTTACTGAATCAGAGTCTCCCTATTTACTTGCTGGTTTCCTTGGGAAGCTTCATGTGATCACGAAGGATGCCAATAACAATGTTGCAGTTCTGCAGGCTTATGTGCCGAACCGTCATGCTTCCACCTCAGCAGCTTCATCCTCATCACCAGATGATGCCTCTGAAATTTCTGAACCACAAGCAGAGTCAGAAGTAGATCTTTGGAAGGTAATTGCCACAAGGGGTGCACGGACTGCTGAACTGGTTAGCTGTCTCGTCCTTGATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

476

Amino Acids

52.62

Weight (kDa)

6.49

Isoelectric Point (pI)

52.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 79 5.6e-07 F-box domain
Kelch_FKB95 PF25210 168 - 407 4.6e-13 FKB95, Kelch-repeats domain
Kelch_1 PF01344 171 - 216 3.2e-13 Kelch motif
Beta-prop_Calicin PF13964 176 - 268 8.2e-08 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 176 - 266 8.8e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 219 - 267 3.5e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 617
AciI CCGC 3 cut(s) 237, 461, 601
AclWI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
AcoI YGGCCR 1 cut(s) 917
AcsI RAATTY 3 cut(s) 243, 964, 1326
AcuI CTGAAG 2 cut(s) 411, 771
AdeI CACNNNGTG 1 cut(s) 68
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 3 cut(s) 109, 518, 770
AgsI TTSAA 8 cut(s) 22, 193, 199, 250, 308, 447, 573, 743
AjiI CACGTC 1 cut(s) 722
AjnI CCWGG 1 cut(s) 596
AluBI AGCT 6 cut(s) 319, 749, 812, 1210, 1295, 1410
AluI AGCT 6 cut(s) 319, 749, 812, 1210, 1295, 1410
Alw21I GWGCWC 1 cut(s) 1390
Alw26I GTCTC 2 cut(s) 1182, 1418
Alw44I GTGCAC 1 cut(s) 1386
AlwI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 3 cut(s) 732, 917, 1004
ApaLI GTGCAC 1 cut(s) 1386
ApeKI GCWGC 3 cut(s) 347, 546, 1292
ApoI RAATTY 3 cut(s) 243, 964, 1326
Asp700I GAANNNNTTC 1 cut(s) 1146
AspA2I CCTAGG 2 cut(s) 176, 559
AspS9I GGNCC 4 cut(s) 47, 337, 733, 780
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 5 cut(s) 953, 973, 986, 1061, 1299
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 3 cut(s) 47, 337, 780
AvrII CCTAGG 2 cut(s) 176, 559
BaeGI GKGCMC 1 cut(s) 1390
BamHI GGATCC 2 cut(s) 180, 1066
BbsI GAAGAC 2 cut(s) 87, 128
Bbv12I GWGCWC 1 cut(s) 1390
BbvCI CCTCAGC 1 cut(s) 1288
BbvI GCAGC 3 cut(s) 359, 533, 1304
BccI CCATC 4 cut(s) 196, 536, 1040, 1078
BceAI ACGGC 2 cut(s) 512, 904
BciT130I CCWGG 1 cut(s) 598
BclI TGATCA 1 cut(s) 1218
BcnI CCSGG 1 cut(s) 865
BcoDI GTCTC 2 cut(s) 1182, 1418
BfaI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
BfmI CTRYAG 2 cut(s) 579, 1253
BglI GCCNNNNNGGC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 1357
BisI GCNGC 3 cut(s) 348, 547, 1293
BlnI CCTAGG 2 cut(s) 176, 559
BlsI GCNGC 3 cut(s) 349, 548, 1294
Bme1390I CCNGG 2 cut(s) 598, 865
Bme18I GGWCC 3 cut(s) 47, 337, 780
BmeT110I CYCGRG 1 cut(s) 118
BmgBI CACGTC 1 cut(s) 722
BmgT120I GGNCC 4 cut(s) 47, 337, 733, 780
BmiI GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
BmrFI CCNGG 2 cut(s) 598, 865
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 4 cut(s) 484, 1111, 1219, 1306
BmuI ACTGGG 1 cut(s) 126
BpiI GAAGAC 2 cut(s) 87, 128
BplI GAGNNNNNCTC 2 cut(s) 34, 66
Bpu10I CCTNAGC 2 cut(s) 813, 1288
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 865
BsaAI YACGTR 1 cut(s) 212
BsaJI CCNNGG 3 cut(s) 176, 559, 1201
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 518, 770
Bse1I ACTGG 6 cut(s) 132, 504, 611, 991, 1068, 1407
BseBI CCWGG 1 cut(s) 598
BseDI CCNNGG 3 cut(s) 176, 559, 1201
BseGI GGATG 5 cut(s) 469, 499, 873, 1234, 1298
BseLI CCNNNNNNNGG 3 cut(s) 109, 518, 770
BseMII CTCAG 2 cut(s) 827, 1302
BseNI ACTGG 6 cut(s) 132, 504, 611, 991, 1068, 1407
BseSI GKGCMC 1 cut(s) 1390
BseXI GCAGC 3 cut(s) 359, 533, 1304
BshFI GGCC 3 cut(s) 734, 919, 1006
BsiHKAI GWGCWC 1 cut(s) 1390
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 1 cut(s) 864
BslFI GGGAC 3 cut(s) 766, 1036, 1172
BslI CCNNNNNNNGG 3 cut(s) 109, 518, 770
BsmAI GTCTC 2 cut(s) 1182, 1418
BsmFI GGGAC 3 cut(s) 766, 1036, 1172
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 3 cut(s) 734, 919, 1006
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 1390
BspACI CCGC 3 cut(s) 237, 461, 601
BspANI GGCC 3 cut(s) 734, 919, 1006
BspCNI CTCAG 2 cut(s) 826, 1301
BspLI GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
BspMAI CTGCAG 1 cut(s) 1257
BspPI GGATC 7 cut(s) 175, 188, 510, 755, 947, 1061, 1074
BsrI ACTGG 6 cut(s) 132, 504, 611, 991, 1068, 1407
BssECI CCNNGG 3 cut(s) 176, 559, 1201
BssT1I CCWWGG 3 cut(s) 176, 559, 1201
Bst2UI CCWGG 1 cut(s) 598
Bst4CI ACNGT 3 cut(s) 229, 1042, 1274
Bst6I CTCTTC 1 cut(s) 744
BstAPI GCANNNNNTGC 2 cut(s) 364, 1394
BstBAI YACGTR 1 cut(s) 212
BstC8I GCNNGC 6 cut(s) 479, 797, 917, 1008, 1017, 1257
BstDEI CTNAG 2 cut(s) 813, 1288
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 5 cut(s) 469, 499, 873, 1234, 1298
BstMAI GTCTC 2 cut(s) 1182, 1418
BstMWI GCNNNNNNNGC 8 cut(s) 364, 373, 416, 533, 836, 1012, 1016, 1394
BstNI CCWGG 1 cut(s) 598
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 2 cut(s) 596, 863
BstSFI CTRYAG 2 cut(s) 579, 1253
BstSLI GKGCMC 1 cut(s) 1390
BstV1I GCAGC 3 cut(s) 359, 533, 1304
BstV2I GAAGAC 2 cut(s) 87, 128
BstX2I RGATCY 3 cut(s) 180, 1066, 1357
BstYI RGATCY 3 cut(s) 180, 1066, 1357
BsuRI GGCC 3 cut(s) 734, 919, 1006
BtrI CACGTC 1 cut(s) 722
BtsCI GGATG 5 cut(s) 469, 499, 873, 1234, 1298
BtsIMutI CAGTG 2 cut(s) 497, 1061
Cac8I GCNNGC 6 cut(s) 479, 797, 917, 1008, 1017, 1257
Cfr13I GGNCC 4 cut(s) 47, 337, 733, 780
Csp6I GTAC 1 cut(s) 213
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 2 cut(s) 813, 1288
DraI TTTAAA 1 cut(s) 681
DraIII CACNNNGTG 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 917
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco130I CCWWGG 3 cut(s) 176, 559, 1201
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 3 cut(s) 47, 337, 780
Eco57I CTGAAG 2 cut(s) 411, 771
Eco88I CYCGRG 1 cut(s) 118
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 1 cut(s) 596
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 3 cut(s) 176, 559, 1201
ErhI CCWWGG 3 cut(s) 176, 559, 1201
FalI AAGNNNNNCTT 2 cut(s) 1344, 1376
FaqI GGGAC 3 cut(s) 766, 1036, 1172
FauNDI CATATG 1 cut(s) 323
FbaI TGATCA 1 cut(s) 1218
Fnu4HI GCNGC 3 cut(s) 348, 547, 1293
FokI GGATG 5 cut(s) 476, 506, 880, 1241, 1285
Fsp4HI GCNGC 3 cut(s) 348, 547, 1293
FspBI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
GluI GCNGC 3 cut(s) 348, 547, 1293
HaeIII GGCC 3 cut(s) 734, 919, 1006
HapII CCGG 1 cut(s) 864
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 7 cut(s) 127, 160, 398, 632, 1169, 1175, 1346
HpaII CCGG 1 cut(s) 864
HphI GGTGA 5 cut(s) 953, 973, 986, 1061, 1299
Hpy166II GTNNAC 2 cut(s) 47, 1388
Hpy188I TCNGA 7 cut(s) 159, 455, 489, 1174, 1324, 1333, 1351
Hpy188III TCNNGA 7 cut(s) 140, 335, 389, 956, 1082, 1157, 1222
Hpy8I GTNNAC 2 cut(s) 47, 1388
HpyAV CCTTC 3 cut(s) 484, 1219, 1360
HpyCH4III ACNGT 3 cut(s) 229, 1042, 1274
HpyCH4IV ACGT 2 cut(s) 211, 721
HpyCH4V TGCA 8 cut(s) 497, 830, 1010, 1061, 1139, 1247, 1255, 1388
HpyF10VI GCNNNNNNNGC 8 cut(s) 364, 373, 416, 533, 836, 1012, 1016, 1394
HpyF3I CTNAG 2 cut(s) 813, 1288
HpySE526I ACGT 2 cut(s) 211, 721
Ksp22I TGATCA 1 cut(s) 1218
Lsp1109I GCAGC 3 cut(s) 359, 533, 1304
LweI GCATC 4 cut(s) 484, 1111, 1219, 1306
MaeI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
MaeII ACGT 2 cut(s) 211, 721
MaeIII GTNAC 4 cut(s) 207, 709, 819, 1036
MboII GAAGA 6 cut(s) 87, 128, 211, 404, 731, 1154
MfeI CAATTG 1 cut(s) 25
MflI RGATCY 3 cut(s) 180, 1066, 1357
MhlI GDGCHC 1 cut(s) 1390
MluCI AATT 9 cut(s) 25, 194, 243, 250, 964, 1052, 1152, 1326, 1371
MlyI GAGTC 2 cut(s) 1184, 1355
MmeI TCCRAC 3 cut(s) 467, 682, 916
MroXI GAANNNNTTC 1 cut(s) 1146
MseI TTAA 3 cut(s) 296, 413, 680
MslI CAYNNNNRTG 2 cut(s) 214, 230
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 1 cut(s) 864
MspR9I CCNGG 2 cut(s) 598, 865
MunI CAATTG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 513
MvaI CCWGG 1 cut(s) 598
MwoI GCNNNNNNNGC 8 cut(s) 364, 373, 416, 533, 836, 1012, 1016, 1394
NciI CCSGG 1 cut(s) 865
NdeI CATATG 1 cut(s) 323
NlaIV GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
NmuCI GTSAC 2 cut(s) 207, 1036
NspI RCATGY 1 cut(s) 799
PaeI GCATGC 1 cut(s) 799
PctI GAATGC 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 1146
PfeI GAWTC 5 cut(s) 127, 160, 398, 632, 1169
PkrI GCNGC 3 cut(s) 349, 548, 1294
PleI GAGTC 2 cut(s) 1183, 1354
PpsI GAGTC 2 cut(s) 1183, 1354
Ppu21I YACGTR 1 cut(s) 212
PsiI TTATAA 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 596
PspGI CCWGG 1 cut(s) 596
PspN4I GGNNCC 5 cut(s) 182, 339, 735, 782, 1068
PspPI GGNCC 4 cut(s) 47, 337, 733, 780
PstI CTGCAG 1 cut(s) 1257
PsuI RGATCY 3 cut(s) 180, 1066, 1357
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
RseI CAYNNNNRTG 2 cut(s) 214, 230
SaqAI TTAA 3 cut(s) 296, 413, 680
SatI GCNGC 3 cut(s) 348, 547, 1293
Sau96I GGNCC 4 cut(s) 47, 337, 733, 780
SchI GAGTC 2 cut(s) 1184, 1355
ScrFI CCNGG 2 cut(s) 598, 865
SduI GDGCHC 1 cut(s) 1390
SfaNI GCATC 4 cut(s) 484, 1111, 1219, 1306
SfcI CTRYAG 2 cut(s) 579, 1253
SinI GGWCC 3 cut(s) 47, 337, 780
SmiMI CAYNNNNRTG 2 cut(s) 214, 230
SmlI CTYRAG 1 cut(s) 848
SmoI CTYRAG 1 cut(s) 848
SphI GCATGC 1 cut(s) 799
Sse9I AATT 9 cut(s) 25, 194, 243, 250, 964, 1052, 1152, 1326, 1371
SsiI CCGC 3 cut(s) 237, 461, 601
SspMI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
StyD4I CCNGG 2 cut(s) 596, 863
StyI CCWWGG 3 cut(s) 176, 559, 1201
TaaI ACNGT 3 cut(s) 229, 1042, 1274
TaiI ACGT 2 cut(s) 214, 724
TaqI TCGA 2 cut(s) 505, 1044
TasI AATT 9 cut(s) 25, 194, 243, 250, 964, 1052, 1152, 1326, 1371
TfiI GAWTC 5 cut(s) 127, 160, 398, 632, 1169
Tru1I TTAA 3 cut(s) 296, 413, 680
Tru9I TTAA 3 cut(s) 296, 413, 680
TscAI CASTG 2 cut(s) 504, 1068
TseFI GTSAC 2 cut(s) 207, 1036
TseI GCWGC 3 cut(s) 347, 546, 1292
Tsp45I GTSAC 2 cut(s) 207, 1036
TspDTI ATGAA 6 cut(s) 87, 411, 864, 957, 1202, 1287
TspGWI ACGGA 1 cut(s) 1405
TspRI CASTG 2 cut(s) 504, 1068
VneI GTGCAC 1 cut(s) 1386
VpaK11BI GGWCC 3 cut(s) 47, 337, 780
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 3 cut(s) 243, 964, 1326
XceI RCATGY 1 cut(s) 799
XmaJI CCTAGG 2 cut(s) 176, 559
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 8 cut(s) 36, 177, 266, 378, 402, 560, 714, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.