Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012625

Physical Location & Seq

Genomic Coordinates Forward (+)

28400001 .. 28402399

Gene Structure 2399 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1221400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGGGGGTATATTGAGCTTGAACCATTCCAAGGCTAGGGTCGGTGAACCGTCTCAAGATTTACGTAGTGCATCTTGCAAGAGACCGAGGTTGTCCCCAAGCTTTTATGACGAAAACCCGAGATTGATTCCCTGTCTTCCTGACGAGATATCTATTCAGATTCTTGCTAGAATTCCTAGGATCCACTATATGAAGCTCAAGTCTGTTTCGAGGACCTGGAAAGCCACCGTTACAAGTGCTGAACTCTTCTCTTTGAGAAAAGAGCTAGGAACAAAGGAGGAATGGCTCTACATGTTAACAAAGGTCCAAAATGATAAGCTCATATGGTATGCACTTGATCCTATGGCCGGAAGATGGCAAAGGTTGCCACCTATGCCTAATGTTACTCTGGAAGATGAATCTAGGAAAGGTTTAACTGGCCAGAGAATGTGGAATATGGCAGGCTCCAGTATCAGAATTGCGGATGCCGTCATGGGGGGATTTTCCAAGGCTTCGGCCCTGAGATGCACATGGCGGTACAATCCAGTTAAAAATACTTGGAGTGAATCAAGTCCAATGTCAATTGGTAGAGCTTATTGTAAGACCGGCATCTTAAACAATAAGCTTTATGTTGTTGGAGGTGTTACAAGGGGTCATGGTGGATTGACCCCTCTTCAATCAGCTGAAGTATTTGATCCAAAAACAGGTGTGTGGTCTCAAGTACCAAGCATGCCTTTTATGAAAGCTCAGGTGTTACCAACTGCATTTTTGGCTGATCTGCTGAAGCCTATTGCTACTGGAATGACTTCATACAGGGGAAGGTTGTTTGTTCCCCAGAGTTTGTACTGCTGGCCATTTTTTGTTGATGTTGGAGGAGAGGTTTATGATCCAGAGGCAAATTCATGGGTTGAAATGCCAATTGGTATGGGCGAGGGTTGGCCTGCGAGGCAGGCAGGGACGAAAATGAGTGTCACAGTTGATGGTGAATTGTATGCACTGGATCCTTCTAGTTCTCTTGACAGTGCAAAGATCAAGGTATACGATTACCAAGCTGATGCCTGGAAGGTTGTTGCAGAAGATGTTCCAATTCGTGACTTCACTGAATCAGAGTCTCCCTATTTGCTTGCTGGATTGCTGGGGAAGCTTCATGTAATCACAAAGGATGCAAATCACAAAATTGCTGTTCTGCAGGCTTCGGTGCAGGACCATTTTGCGTCAAGCTCAGTAGCTTCATCCTCATCACACAATGACCCCCATGAAGATGTTGAACGACCAGCAGAATCAGAACGAGATCTCTGGAAAGTAATTGCCACAAGAAGTGCACGGGCTGCTGACCTGGTTAGTTGTCTAGTCCTTGATGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like Kelch_1

Protein Analysis

448

Amino Acids

49.59

Weight (kDa)

8.38

Isoelectric Point (pI)

52.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	43 - 83	1.4e-07	F-box domain
Kelch_1	PF01344	157 - 187	6.1e-06	Kelch motif
Kelch_FKB95	PF25210	157 - 380	7.3e-12	FKB95, Kelch-repeats domain
Kelch_1	PF01344	190 - 238	3.6e-13	Kelch motif
Kelch_2	PF07646	190 - 238	3.9e-06	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012625)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g30861 FvH4_5g30861
malus_domestica	MD08G1221400.v1.1 MD15G1412100.v1.1
prunus_persica	Prupe.1G558500_v2.0.a1 Prupe.1G558500_v2.0.a1
pyrus_communis	pycom08g19200 pycom15g36690 pycom15g36700
rosa_chinensis	RchiOBHm_Chr7g0226051
rosa_laevigata	RLG00000001769
rosa_multiflora	Rmu_sc0004200.1_g000006
rosa_roxburghii	Rroxscaffold_3G00233290
rosa_rugosa	Rorug07G0234400
rosa_samantha	Rh7AG368600 Rh7BG368700 Rh7CG387200 Rh7DG379800
rosa_wichuraiana	Rw0G020920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1017
AciI	CCGC	2 cut(s)	461, 514
AclWI	GGATC	7 cut(s)	175, 188, 332, 668, 860, 974, 987
AcoI	YGGCCR	3 cut(s)	345, 418, 830
AcsI	RAATTY	2 cut(s)	171, 877
AcuI	CTGAAG	2 cut(s)	684, 782
AfaI	GTAC	3 cut(s)	518, 702, 824
AfiI	CCNNNNNNNGG	6 cut(s)	31, 36, 347, 354, 474, 683
AflIII	ACRYGT	1 cut(s)	291
AgsI	TTSAA	4 cut(s)	22, 656, 890, 1247
AjnI	CCWGG	3 cut(s)	215, 1037, 1314
AjuI	GAANNNNNNNTTGG	2 cut(s)	882, 914
Alw21I	GWGCWC	1 cut(s)	1303
Alw26I	GTCTC	4 cut(s)	57, 76, 699, 1095
Alw44I	GTGCAC	1 cut(s)	1299
AlwI	GGATC	7 cut(s)	175, 188, 332, 668, 860, 974, 987
Ama87I	CYCGRG	1 cut(s)	118
AoxI	GGCC	5 cut(s)	345, 418, 495, 830, 917
ApaLI	GTGCAC	1 cut(s)	1299
ApeKI	GCWGC	1 cut(s)	1307
ApoI	RAATTY	2 cut(s)	171, 877
Asp700I	GAANNNNTTC	2 cut(s)	784, 1059
AspA2I	CCTAGG	1 cut(s)	176
AspS9I	GGNCC	4 cut(s)	213, 304, 496, 1183
AsuHPI	GGTGA	2 cut(s)	56, 974
AvaI	CYCGRG	1 cut(s)	118
AvaII	GGWCC	3 cut(s)	213, 304, 1183
AvrII	CCTAGG	1 cut(s)	176
BaeGI	GKGCMC	1 cut(s)	1303
BalI	TGGCCA	2 cut(s)	420, 832
BamHI	GGATCC	2 cut(s)	180, 979
BbsI	GAAGAC	1 cut(s)	128
Bbv12I	GWGCWC	1 cut(s)	1303
BbvI	GCAGC	1 cut(s)	1294
BccI	CCATC	2 cut(s)	348, 953
BceAI	ACGGC	1 cut(s)	452
BciT130I	CCWGG	3 cut(s)	217, 1039, 1316
BcoDI	GTCTC	4 cut(s)	57, 76, 699, 1095
BfaI	CTAG	7 cut(s)	36, 168, 177, 266, 402, 987, 1328
BfmI	CTRYAG	1 cut(s)	1166
BglI	GCCNNNNNGGC	1 cut(s)	925
BglII	AGATCT	1 cut(s)	1270
BisI	GCNGC	1 cut(s)	1308
BlnI	CCTAGG	1 cut(s)	176
BlsI	GCNGC	1 cut(s)	1309
Bme1390I	CCNGG	3 cut(s)	217, 1039, 1316
Bme18I	GGWCC	3 cut(s)	213, 304, 1183
BmeT110I	CYCGRG	1 cut(s)	118
BmgT120I	GGNCC	4 cut(s)	213, 304, 496, 1183
BmiI	GGNNCC	3 cut(s)	182, 445, 981
BmrFI	CCNGG	3 cut(s)	217, 1039, 1316
BmsI	GCATC	6 cut(s)	80, 454, 494, 597, 1024, 1132
BpiI	GAAGAC	1 cut(s)	128
BpmI	CTGGAG	1 cut(s)	430
Bpu10I	CCTNAGC	1 cut(s)	726
BpuEI	CTTGAG	3 cut(s)	39, 182, 681
BsaAI	YACGTR	1 cut(s)	65
BsaBI	GATNNNNATC	1 cut(s)	1146
BsaI	GGTCTC	2 cut(s)	76, 699
BsaJI	CCNNGG	4 cut(s)	30, 86, 176, 486
BsaXI	ACNNNNNCTCC	2 cut(s)	843, 873
Bsc4I	CCNNNNNNNGG	6 cut(s)	31, 36, 347, 354, 474, 683
Bse118I	RCCGGY	1 cut(s)	584
Bse1I	ACTGG	5 cut(s)	421, 447, 524, 781, 981
Bse8I	GATNNNNATC	1 cut(s)	1146
BseBI	CCWGG	3 cut(s)	217, 1039, 1316
BseDI	CCNNGG	4 cut(s)	30, 86, 176, 486
BseGI	GGATG	3 cut(s)	469, 1147, 1211
BseJI	GATNNNNATC	1 cut(s)	1146
BseLI	CCNNNNNNNGG	6 cut(s)	31, 36, 347, 354, 474, 683
BseMII	CTCAG	3 cut(s)	491, 740, 1215
BseNI	ACTGG	5 cut(s)	421, 447, 524, 781, 981
BseRI	GAGGAG	1 cut(s)	867
BseSI	GKGCMC	1 cut(s)	1303
BseXI	GCAGC	1 cut(s)	1294
BseYI	CCCAGC	1 cut(s)	1114
BsgI	GTGCAG	1 cut(s)	1199
BshFI	GGCC	5 cut(s)	347, 420, 497, 832, 919
BsiHKAI	GWGCWC	1 cut(s)	1303
BsiHKCI	CYCGRG	1 cut(s)	118
BsiSI	CCGG	2 cut(s)	348, 585
BslFI	GGGAC	2 cut(s)	79, 949
BslI	CCNNNNNNNGG	6 cut(s)	31, 36, 347, 354, 474, 683
BsmAI	GTCTC	4 cut(s)	57, 76, 699, 1095
BsmBI	CGTCTC	1 cut(s)	57
BsmFI	GGGAC	2 cut(s)	79, 949
BsnI	GGCC	5 cut(s)	347, 420, 497, 832, 919
Bso31I	GGTCTC	2 cut(s)	76, 699
BsoBI	CYCGRG	1 cut(s)	118
Bsp1286I	GDGCHC	1 cut(s)	1303
Bsp143I	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
BspACI	CCGC	2 cut(s)	461, 514
BspANI	GGCC	5 cut(s)	347, 420, 497, 832, 919
BspCNI	CTCAG	3 cut(s)	492, 739, 1214
BspLI	GGNNCC	3 cut(s)	182, 445, 981
BspMAI	CTGCAG	1 cut(s)	1170
BspPI	GGATC	7 cut(s)	175, 188, 332, 668, 860, 974, 987
BspTNI	GGTCTC	2 cut(s)	76, 699
BsrFI	RCCGGY	1 cut(s)	584
BsrI	ACTGG	5 cut(s)	421, 447, 524, 781, 981
BssAI	RCCGGY	1 cut(s)	584
BssECI	CCNNGG	4 cut(s)	30, 86, 176, 486
BssMI	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
BssNAI	GTATAC	1 cut(s)	1018
BssT1I	CCWWGG	3 cut(s)	30, 176, 486
Bst1107I	GTATAC	1 cut(s)	1018
Bst2UI	CCWGG	3 cut(s)	217, 1039, 1316
Bst4CI	ACNGT	4 cut(s)	51, 229, 955, 1001
Bst6I	CTCTTC	2 cut(s)	251, 657
BstAPI	GCANNNNNTGC	2 cut(s)	364, 1307
BstBAI	YACGTR	1 cut(s)	65
BstC8I	GCNNGC	7 cut(s)	442, 710, 830, 921, 930, 1104, 1170
BstDEI	CTNAG	3 cut(s)	500, 726, 1201
BstF5I	GGATG	3 cut(s)	469, 1147, 1211
BstKTI	GATC	8 cut(s)	183, 340, 676, 757, 868, 982, 1011, 1273
BstMAI	GTCTC	4 cut(s)	57, 76, 699, 1095
BstMBI	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
BstMWI	GCNNNNNNNGC	7 cut(s)	364, 373, 749, 925, 929, 1120, 1307
BstNI	CCWGG	3 cut(s)	217, 1039, 1316
BstNSI	RCATGY	2 cut(s)	295, 712
BstSCI	CCNGG	3 cut(s)	215, 1037, 1314
BstSFI	CTRYAG	1 cut(s)	1166
BstSLI	GKGCMC	1 cut(s)	1303
BstSNI	TACGTA	1 cut(s)	65
BstV1I	GCAGC	1 cut(s)	1294
BstV2I	GAAGAC	1 cut(s)	128
BstX2I	RGATCY	3 cut(s)	180, 979, 1270
BstYI	RGATCY	3 cut(s)	180, 979, 1270
BstZ17I	GTATAC	1 cut(s)	1018
BsuRI	GGCC	5 cut(s)	347, 420, 497, 832, 919
BtsCI	GGATG	3 cut(s)	469, 1147, 1211
BtsIMutI	CAGTG	3 cut(s)	974, 1006, 1077
Cac8I	GCNNGC	7 cut(s)	442, 710, 830, 921, 930, 1104, 1170
Cfr10I	RCCGGY	1 cut(s)	584
Cfr13I	GGNCC	4 cut(s)	213, 304, 496, 1183
CseI	GACGC	1 cut(s)	1182
CsiI	ACCWGGT	1 cut(s)	1314
Csp6I	GTAC	3 cut(s)	517, 701, 823
CviAII	CATG	8 cut(s)	292, 472, 510, 635, 709, 882, 1127, 1235
CviQI	GTAC	3 cut(s)	517, 701, 823
DdeI	CTNAG	3 cut(s)	500, 726, 1201
DpnI	GATC	8 cut(s)	182, 339, 675, 756, 867, 981, 1010, 1272
DpnII	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
EaeI	YGGCCR	3 cut(s)	345, 418, 830
Eam1104I	CTCTTC	2 cut(s)	251, 657
EarI	CTCTTC	2 cut(s)	251, 657
Eco105I	TACGTA	1 cut(s)	65
Eco130I	CCWWGG	3 cut(s)	30, 176, 486
Eco31I	GGTCTC	2 cut(s)	76, 699
Eco32I	GATATC	1 cut(s)	150
Eco47I	GGWCC	3 cut(s)	213, 304, 1183
Eco57I	CTGAAG	2 cut(s)	684, 782
Eco88I	CYCGRG	1 cut(s)	118
EcoO109I	RGGNCCY	1 cut(s)	213
EcoRI	GAATTC	1 cut(s)	171
EcoRII	CCWGG	3 cut(s)	215, 1037, 1314
EcoRV	GATATC	1 cut(s)	150
EcoT14I	CCWWGG	3 cut(s)	30, 176, 486
ErhI	CCWWGG	3 cut(s)	30, 176, 486
Esp3I	CGTCTC	1 cut(s)	57
FaeI	CATG	8 cut(s)	295, 475, 513, 638, 712, 885, 1130, 1238
FalI	AAGNNNNNCTT	2 cut(s)	697, 729
FaqI	GGGAC	2 cut(s)	79, 949
FatI	CATG	8 cut(s)	291, 471, 509, 634, 708, 881, 1126, 1234
FauNDI	CATATG	1 cut(s)	323
FblI	GTMKAC	1 cut(s)	1017
Fnu4HI	GCNGC	1 cut(s)	1308
FokI	GGATG	3 cut(s)	476, 1154, 1198
Fsp4HI	GCNGC	1 cut(s)	1308
FspBI	CTAG	7 cut(s)	36, 168, 177, 266, 402, 987, 1328
GluI	GCNGC	1 cut(s)	1308
GsaI	CCCAGC	1 cut(s)	1118
GsuI	CTGGAG	1 cut(s)	430
HaeIII	GGCC	5 cut(s)	347, 420, 497, 832, 919
HapII	CCGG	2 cut(s)	348, 585
HgaI	GACGC	1 cut(s)	1182
Hin1II	CATG	8 cut(s)	295, 475, 513, 638, 712, 885, 1130, 1238
HincII	GTYRAC	1 cut(s)	297
HindII	GTYRAC	1 cut(s)	297
HindIII	AAGCTT	3 cut(s)	100, 602, 1121
HinfI	GANTC	7 cut(s)	127, 160, 398, 545, 1082, 1088, 1259
HpaI	GTTAAC	1 cut(s)	297
HpaII	CCGG	2 cut(s)	348, 585
HphI	GGTGA	2 cut(s)	56, 974
Hpy166II	GTNNAC	4 cut(s)	47, 297, 1018, 1301
Hpy188I	TCNGA	4 cut(s)	159, 455, 1087, 1264
Hpy188III	TCNNGA	7 cut(s)	56, 140, 389, 869, 995, 1070, 1276
Hpy8I	GTNNAC	4 cut(s)	47, 297, 1018, 1301
HpyAV	CCTTC	3 cut(s)	792, 993, 1036
HpyCH4III	ACNGT	4 cut(s)	51, 229, 955, 1001
HpyCH4IV	ACGT	1 cut(s)	64
HpyF10VI	GCNNNNNNNGC	7 cut(s)	364, 373, 749, 925, 929, 1120, 1307
HpyF3I	CTNAG	3 cut(s)	500, 726, 1201
HpySE526I	ACGT	1 cut(s)	64
Hsp92II	CATG	8 cut(s)	295, 475, 513, 638, 712, 885, 1130, 1238
KspAI	GTTAAC	1 cut(s)	297
Kzo9I	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
LmnI	GCTCC	1 cut(s)	449
Lsp1109I	GCAGC	1 cut(s)	1294
LweI	GCATC	6 cut(s)	80, 454, 494, 597, 1024, 1132
MabI	ACCWGGT	1 cut(s)	1314
MaeI	CTAG	7 cut(s)	36, 168, 177, 266, 402, 987, 1328
MaeII	ACGT	1 cut(s)	64
MaeIII	GTNAC	6 cut(s)	229, 382, 622, 732, 949, 1070
MalI	GATC	8 cut(s)	182, 339, 675, 756, 867, 981, 1010, 1272
MboI	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
MboII	GAAGA	7 cut(s)	128, 238, 363, 404, 644, 1067, 1250
MfeI	CAATTG	2 cut(s)	561, 897
MflI	RGATCY	3 cut(s)	180, 979, 1270
MhlI	GDGCHC	1 cut(s)	1303
MlsI	TGGCCA	2 cut(s)	420, 832
MluCI	AATT	9 cut(s)	171, 456, 561, 877, 897, 965, 1065, 1155, 1284
MluNI	TGGCCA	2 cut(s)	420, 832
MlyI	GAGTC	1 cut(s)	1097
MmeI	TCCRAC	2 cut(s)	595, 829
Mox20I	TGGCCA	2 cut(s)	420, 832
MroXI	GAANNNNTTC	2 cut(s)	784, 1059
MscI	TGGCCA	2 cut(s)	420, 832
MseI	TTAA	4 cut(s)	296, 413, 528, 593
MslI	CAYNNNNRTG	1 cut(s)	1239
Msp20I	TGGCCA	2 cut(s)	420, 832
MspA1I	CMGCKG	1 cut(s)	662
MspI	CCGG	2 cut(s)	348, 585
MspR9I	CCNGG	3 cut(s)	217, 1039, 1316
MunI	CAATTG	2 cut(s)	561, 897
MvaI	CCWGG	3 cut(s)	217, 1039, 1316
MwoI	GCNNNNNNNGC	7 cut(s)	364, 373, 749, 925, 929, 1120, 1307
NdeI	CATATG	1 cut(s)	323
NdeII	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
NlaIII	CATG	8 cut(s)	295, 475, 513, 638, 712, 885, 1130, 1238
NlaIV	GGNNCC	3 cut(s)	182, 445, 981
NmuCI	GTSAC	2 cut(s)	949, 1070
NspI	RCATGY	2 cut(s)	295, 712
PaeI	GCATGC	1 cut(s)	712
PciI	ACATGT	1 cut(s)	291
PdmI	GAANNNNTTC	2 cut(s)	784, 1059
PfeI	GAWTC	6 cut(s)	127, 160, 398, 545, 1082, 1259
PkrI	GCNGC	1 cut(s)	1309
PleI	GAGTC	1 cut(s)	1096
PpsI	GAGTC	1 cut(s)	1096
Ppu21I	YACGTR	1 cut(s)	65
PpuMI	RGGWCCY	1 cut(s)	213
PscI	ACATGT	1 cut(s)	291
Psp5II	RGGWCCY	1 cut(s)	213
Psp6I	CCWGG	3 cut(s)	215, 1037, 1314
PspFI	CCCAGC	1 cut(s)	1114
PspGI	CCWGG	3 cut(s)	215, 1037, 1314
PspN4I	GGNNCC	3 cut(s)	182, 445, 981
PspPI	GGNCC	4 cut(s)	213, 304, 496, 1183
PspPPI	RGGWCCY	1 cut(s)	213
PstI	CTGCAG	1 cut(s)	1170
PsuI	RGATCY	3 cut(s)	180, 979, 1270
PvuII	CAGCTG	1 cut(s)	662
RsaI	GTAC	3 cut(s)	518, 702, 824
RsaNI	GTAC	3 cut(s)	517, 701, 823
RseI	CAYNNNNRTG	1 cut(s)	1239
SaqAI	TTAA	4 cut(s)	296, 413, 528, 593
SatI	GCNGC	1 cut(s)	1308
Sau3AI	GATC	8 cut(s)	180, 337, 673, 754, 865, 979, 1008, 1270
Sau96I	GGNCC	4 cut(s)	213, 304, 496, 1183
SchI	GAGTC	1 cut(s)	1097
ScrFI	CCNGG	3 cut(s)	217, 1039, 1316
SduI	GDGCHC	1 cut(s)	1303
SexAI	ACCWGGT	1 cut(s)	1314
SfaNI	GCATC	6 cut(s)	80, 454, 494, 597, 1024, 1132
SfcI	CTRYAG	1 cut(s)	1166
SinI	GGWCC	3 cut(s)	213, 304, 1183
SmiMI	CAYNNNNRTG	1 cut(s)	1239
SmlI	CTYRAG	3 cut(s)	54, 197, 696
SmoI	CTYRAG	3 cut(s)	54, 197, 696
SnaBI	TACGTA	1 cut(s)	65
SphI	GCATGC	1 cut(s)	712
Sse9I	AATT	9 cut(s)	171, 456, 561, 877, 897, 965, 1065, 1155, 1284
SsiI	CCGC	2 cut(s)	461, 514
SspMI	CTAG	7 cut(s)	36, 168, 177, 266, 402, 987, 1328
StyD4I	CCNGG	3 cut(s)	215, 1037, 1314
StyI	CCWWGG	3 cut(s)	30, 176, 486
TaaI	ACNGT	4 cut(s)	51, 229, 955, 1001
TaiI	ACGT	1 cut(s)	67
TaqI	TCGA	1 cut(s)	209
TaqII	GACCGA	1 cut(s)	100
TasI	AATT	9 cut(s)	171, 456, 561, 877, 897, 965, 1065, 1155, 1284
TatI	WGTACW	1 cut(s)	822
TfiI	GAWTC	6 cut(s)	127, 160, 398, 545, 1082, 1259
Tru1I	TTAA	4 cut(s)	296, 413, 528, 593
Tru9I	TTAA	4 cut(s)	296, 413, 528, 593
TscAI	CASTG	3 cut(s)	981, 1006, 1084
TseFI	GTSAC	2 cut(s)	949, 1070
TseI	GCWGC	1 cut(s)	1307
Tsp45I	GTSAC	2 cut(s)	949, 1070
TspDTI	ATGAA	8 cut(s)	206, 411, 734, 777, 870, 1115, 1200, 1251
TspRI	CASTG	3 cut(s)	981, 1006, 1084
VneI	GTGCAC	1 cut(s)	1299
VpaK11BI	GGWCC	3 cut(s)	213, 304, 1183
XapI	RAATTY	2 cut(s)	171, 877
XceI	RCATGY	2 cut(s)	295, 712
XmaJI	CCTAGG	1 cut(s)	176
XmiI	GTMKAC	1 cut(s)	1017
XmnI	GAANNNNTTC	2 cut(s)	784, 1059
XspI	CTAG	7 cut(s)	36, 168, 177, 266, 402, 987, 1328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.