Rroxscaffold_3G00233290

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012625
Physical Location & Seq
Forward (+)
19229941 .. 19232457
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233290.1

Sequence Viewer

Length: 1383 bp
ATGGGGTTTCACAGAGTGAAACACGCAAGAGGCAGAGGTTGTCTTCATGCCCTTTATGAGGAAAACCCGAGATTGATTCCCAGTCTTCCTGACGAGATATCTATTCAGATTCTTGCCAGAATACCTAGGATCCACTATTTGAAATTGAAGATGGTGTCACGCACCTGGAAAACCACCGTTATGACTGCCGAACTTTTCAATTTGAGAAAAGAACTAGGAACAACAGAGGAATGGCTCTATGTATTAACAAAGGTTGAAAGTGATAAGCTCATGTGGTATGCTCTGGACCCTCTGGCCGCAAAATGGCAAAGGTTGCCACCAATGCCTAGTGTTTGTCCTGAAGATGAATCTAGGAGGGCTTTAACTGCTCGTAGAATGTGGAAAATGGTAGGTTCAAGTATCAGACTTGCAGATGTCATCATGGGCTGGCTTGGTCGGAAGGATGCACTGGATCGAATGCCTTTTTGTGGTTGTGCCGTTGGTGCTGTTGATGGCTGCCTCTATGTCCTAGGTGGATTTTCAAGGGCTGCAGCCCTATCATGTGCCTGGCGGTATAATCCAGTTATAAACTGTTGGAGTGAATCAACTCCAATGTCAATGGGTAGAGCGTATTGTAAGACAGGCATTTTAAACAATAAACTTTATGTTGTTGGAGGGGTTACTAGAGGACGTGGTGGATTGGCCCCTCTTCAATCAGCTGAAGTATTTGATCCAAAAACAGGTATATGGTCCCAAATACCAAGCATGCCCTTTGCCAAAGCTCAGGTGTTACCAACTGCATTTTTGGCTGATCTGCTCAAGCCTATTGCTACCGGGATGACTTCATACAAGGGGAGATTGTTTGTTCCACAAAGTTTATACTGCTGGCCGTTTTTTGTTGATGTTGGAGGTGAGGTTTATGATCCAGAGGTGAATTCATGGGGTGATATGCCAACTGGCATGGGGGAGGGTTGGCCTGCAAGGCAAGCAGGGACGAAAATGAGTGTCACAGTCGATGGTGAATTGTATGCACTGGATCCTTCTAGTTCTCTTGACAATGCCAAGATCAAGTTATACGATTACCAATATGATGCTTGGAATGTTGTTGCAGAAGATGTTCCAATTCGGGACTTTACTGAATCAGAGTCTCCCTATTTACTTGCTGGTTTCCTTGGGAAGCTTCATGTGATCACGAAGGATAACAATAACAATGTTGCAGTTCTGCAGGCTTATGTGCCGAACCGTCATGCTTCCACCTCAGCAGCTTCATCCTCATCACCAGATGATGCCTCTGAAATTTCTGAACCACAAGCAGAGTCAGAAGTAGATCTTTGGAAGGTAATTGCCACAAGGAGTGCATGGACTGCTGAACTGGTTAGCTGCTGCCTCGTCCTTGATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

460

Amino Acids

51.06

Weight (kDa)

6.62

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 64 4.6e-07 F-box domain
Kelch_FKB95 PF25210 151 - 393 1.6e-12 FKB95, Kelch-repeats domain
Kelch_1 PF01344 154 - 199 1.3e-12 Kelch motif
Beta-prop_Calicin PF13964 159 - 251 1e-07 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 159 - 249 1.8e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 202 - 250 3.4e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AciI CCGC 2 cut(s) 297, 550
AclWI GGATC 7 cut(s) 124, 137, 459, 704, 896, 1010, 1023
AcoI YGGCCR 2 cut(s) 294, 866
AcsI RAATTY 2 cut(s) 913, 1275
AcuI CTGAAG 2 cut(s) 360, 720
AdeI CACNNNGTG 1 cut(s) 16
AfiI CCNNNNNNNGG 4 cut(s) 58, 303, 467, 719
AgsI TTSAA 7 cut(s) 142, 148, 199, 257, 396, 522, 692
AjiI CACGTC 1 cut(s) 671
AjnI CCWGG 2 cut(s) 164, 545
AluBI AGCT 6 cut(s) 268, 698, 761, 1159, 1244, 1359
AluI AGCT 6 cut(s) 268, 698, 761, 1159, 1244, 1359
Alw26I GTCTC 1 cut(s) 1131
AlwI GGATC 7 cut(s) 124, 137, 459, 704, 896, 1010, 1023
Ama87I CYCGRG 1 cut(s) 67
AoxI GGCC 4 cut(s) 294, 681, 866, 953
ApeKI GCWGC 6 cut(s) 495, 527, 530, 1241, 1359, 1362
ApoI RAATTY 2 cut(s) 913, 1275
Asp700I GAANNNNTTC 1 cut(s) 1095
AspA2I CCTAGG 2 cut(s) 125, 508
AspS9I GGNCC 3 cut(s) 286, 682, 729
AsuC2I CCSGG 1 cut(s) 814
AsuHPI GGTGA 5 cut(s) 902, 922, 935, 1010, 1248
AvaI CYCGRG 1 cut(s) 67
AvaII GGWCC 2 cut(s) 286, 729
AvrII CCTAGG 2 cut(s) 125, 508
BamHI GGATCC 2 cut(s) 129, 1015
BbsI GAAGAC 2 cut(s) 35, 77
BbvCI CCTCAGC 1 cut(s) 1237
BbvI GCAGC 6 cut(s) 482, 514, 542, 1253, 1346, 1349
BccI CCATC 3 cut(s) 145, 485, 989
BceAI ACGGC 2 cut(s) 461, 853
BciT130I CCWGG 2 cut(s) 166, 547
BclI TGATCA 1 cut(s) 1167
BcnI CCSGG 1 cut(s) 814
BcoDI GTCTC 1 cut(s) 1131
BfaI CTAG 7 cut(s) 126, 215, 327, 351, 509, 663, 1023
BfmI CTRYAG 2 cut(s) 528, 1202
BglI GCCNNNNNGGC 1 cut(s) 961
BglII AGATCT 1 cut(s) 1306
BisI GCNGC 7 cut(s) 297, 496, 528, 531, 1242, 1360, 1363
BlnI CCTAGG 2 cut(s) 125, 508
BlsI GCNGC 7 cut(s) 298, 497, 529, 532, 1243, 1361, 1364
Bme1390I CCNGG 3 cut(s) 166, 547, 814
Bme18I GGWCC 2 cut(s) 286, 729
BmeT110I CYCGRG 1 cut(s) 67
BmgBI CACGTC 1 cut(s) 671
BmgT120I GGNCC 3 cut(s) 286, 682, 729
BmiI GGNNCC 5 cut(s) 131, 288, 684, 731, 1017
BmrFI CCNGG 3 cut(s) 166, 547, 814
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 3 cut(s) 433, 1060, 1255
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 2 cut(s) 35, 77
Bpu10I CCTNAGC 2 cut(s) 762, 1237
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 1 cut(s) 814
BsaJI CCNNGG 3 cut(s) 125, 508, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 303, 467, 719
Bse1I ACTGG 6 cut(s) 81, 453, 560, 940, 1017, 1356
BseBI CCWGG 2 cut(s) 166, 547
BseDI CCNNGG 3 cut(s) 125, 508, 1150
BseGI GGATG 3 cut(s) 448, 822, 1247
BseLI CCNNNNNNNGG 4 cut(s) 58, 303, 467, 719
BseMII CTCAG 2 cut(s) 776, 1251
BseNI ACTGG 6 cut(s) 81, 453, 560, 940, 1017, 1356
BseXI GCAGC 6 cut(s) 482, 514, 542, 1253, 1346, 1349
BshFI GGCC 4 cut(s) 296, 683, 868, 955
BsiHKCI CYCGRG 1 cut(s) 67
BsiSI CCGG 1 cut(s) 813
BslFI GGGAC 3 cut(s) 715, 985, 1121
BslI CCNNNNNNNGG 4 cut(s) 58, 303, 467, 719
BsmAI GTCTC 1 cut(s) 1131
BsmFI GGGAC 3 cut(s) 715, 985, 1121
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 4 cut(s) 296, 683, 868, 955
BsoBI CYCGRG 1 cut(s) 67
BspACI CCGC 2 cut(s) 297, 550
BspANI GGCC 4 cut(s) 296, 683, 868, 955
BspCNI CTCAG 2 cut(s) 775, 1250
BspLI GGNNCC 5 cut(s) 131, 288, 684, 731, 1017
BspMAI CTGCAG 2 cut(s) 532, 1206
BspPI GGATC 7 cut(s) 124, 137, 459, 704, 896, 1010, 1023
BsrI ACTGG 6 cut(s) 81, 453, 560, 940, 1017, 1356
BssECI CCNNGG 3 cut(s) 125, 508, 1150
BssT1I CCWWGG 3 cut(s) 125, 508, 1150
Bst2UI CCWGG 2 cut(s) 166, 547
Bst4CI ACNGT 4 cut(s) 178, 572, 991, 1223
Bst6I CTCTTC 1 cut(s) 693
BstAPI GCANNNNNTGC 2 cut(s) 313, 1343
BstC8I GCNNGC 6 cut(s) 428, 746, 866, 957, 966, 1206
BstDEI CTNAG 2 cut(s) 762, 1237
BstENI CCTNNNNNAGG 1 cut(s) 56
BstF5I GGATG 3 cut(s) 448, 822, 1247
BstMAI GTCTC 1 cut(s) 1131
BstMWI GCNNNNNNNGC 8 cut(s) 313, 322, 365, 482, 785, 961, 965, 1343
BstNI CCWGG 2 cut(s) 166, 547
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 3 cut(s) 164, 545, 812
BstSFI CTRYAG 2 cut(s) 528, 1202
BstV1I GCAGC 6 cut(s) 482, 514, 542, 1253, 1346, 1349
BstV2I GAAGAC 2 cut(s) 35, 77
BstX2I RGATCY 3 cut(s) 129, 1015, 1306
BstYI RGATCY 3 cut(s) 129, 1015, 1306
BsuRI GGCC 4 cut(s) 296, 683, 868, 955
BtrI CACGTC 1 cut(s) 671
BtsCI GGATG 3 cut(s) 448, 822, 1247
BtsIMutI CAGTG 2 cut(s) 446, 1010
Cac8I GCNNGC 6 cut(s) 428, 746, 866, 957, 966, 1206
Cfr13I GGNCC 3 cut(s) 286, 682, 729
DdeI CTNAG 2 cut(s) 762, 1237
DraI TTTAAA 1 cut(s) 630
DraIII CACNNNGTG 1 cut(s) 16
EaeI YGGCCR 2 cut(s) 294, 866
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco130I CCWWGG 3 cut(s) 125, 508, 1150
Eco32I GATATC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 286, 729
Eco57I CTGAAG 2 cut(s) 360, 720
Eco88I CYCGRG 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 2 cut(s) 164, 545
EcoRV GATATC 1 cut(s) 99
EcoT14I CCWWGG 3 cut(s) 125, 508, 1150
ErhI CCWWGG 3 cut(s) 125, 508, 1150
FalI AAGNNNNNCTT 2 cut(s) 1293, 1325
FaqI GGGAC 3 cut(s) 715, 985, 1121
FbaI TGATCA 1 cut(s) 1167
Fnu4HI GCNGC 7 cut(s) 297, 496, 528, 531, 1242, 1360, 1363
FokI GGATG 3 cut(s) 455, 829, 1234
Fsp4HI GCNGC 7 cut(s) 297, 496, 528, 531, 1242, 1360, 1363
FspBI CTAG 7 cut(s) 126, 215, 327, 351, 509, 663, 1023
GluI GCNGC 7 cut(s) 297, 496, 528, 531, 1242, 1360, 1363
HaeIII GGCC 4 cut(s) 296, 683, 868, 955
HapII CCGG 1 cut(s) 813
HindIII AAGCTT 1 cut(s) 1157
HinfI GANTC 7 cut(s) 76, 109, 347, 581, 1118, 1124, 1295
HpaII CCGG 1 cut(s) 813
HphI GGTGA 5 cut(s) 902, 922, 935, 1010, 1248
Hpy188I TCNGA 7 cut(s) 108, 404, 438, 1123, 1273, 1282, 1300
Hpy188III TCNNGA 7 cut(s) 89, 284, 338, 905, 1031, 1106, 1171
HpyAV CCTTC 4 cut(s) 433, 1029, 1168, 1309
HpyCH4III ACNGT 4 cut(s) 178, 572, 991, 1223
HpyCH4IV ACGT 1 cut(s) 670
HpyF10VI GCNNNNNNNGC 8 cut(s) 313, 322, 365, 482, 785, 961, 965, 1343
HpyF3I CTNAG 2 cut(s) 762, 1237
HpySE526I ACGT 1 cut(s) 670
Ksp22I TGATCA 1 cut(s) 1167
Lsp1109I GCAGC 6 cut(s) 482, 514, 542, 1253, 1346, 1349
LweI GCATC 3 cut(s) 433, 1060, 1255
MaeI CTAG 7 cut(s) 126, 215, 327, 351, 509, 663, 1023
MaeII ACGT 1 cut(s) 670
MaeIII GTNAC 4 cut(s) 156, 658, 768, 985
MboII GAAGA 6 cut(s) 35, 77, 160, 353, 680, 1103
MflI RGATCY 3 cut(s) 129, 1015, 1306
MluCI AATT 7 cut(s) 143, 199, 913, 1001, 1101, 1275, 1320
MlyI GAGTC 2 cut(s) 1133, 1304
MmeI TCCRAC 4 cut(s) 416, 554, 631, 865
MroXI GAANNNNTTC 1 cut(s) 1095
MseI TTAA 3 cut(s) 245, 362, 629
MslI CAYNNNNRTG 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 698
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 3 cut(s) 166, 547, 814
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 2 cut(s) 166, 547
MwoI GCNNNNNNNGC 8 cut(s) 313, 322, 365, 482, 785, 961, 965, 1343
NciI CCSGG 1 cut(s) 814
NlaIV GGNNCC 5 cut(s) 131, 288, 684, 731, 1017
NmuCI GTSAC 2 cut(s) 156, 985
NspI RCATGY 1 cut(s) 748
PaeI GCATGC 1 cut(s) 748
PctI GAATGC 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 5 cut(s) 76, 109, 347, 581, 1118
PkrI GCNGC 7 cut(s) 298, 497, 529, 532, 1243, 1361, 1364
PleI GAGTC 2 cut(s) 1132, 1303
PpsI GAGTC 2 cut(s) 1132, 1303
PsiI TTATAA 1 cut(s) 566
Psp6I CCWGG 2 cut(s) 164, 545
PspGI CCWGG 2 cut(s) 164, 545
PspN4I GGNNCC 5 cut(s) 131, 288, 684, 731, 1017
PspPI GGNCC 3 cut(s) 286, 682, 729
PstI CTGCAG 2 cut(s) 532, 1206
PsuI RGATCY 3 cut(s) 129, 1015, 1306
PvuII CAGCTG 1 cut(s) 698
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 3 cut(s) 245, 362, 629
SatI GCNGC 7 cut(s) 297, 496, 528, 531, 1242, 1360, 1363
Sau96I GGNCC 3 cut(s) 286, 682, 729
SchI GAGTC 2 cut(s) 1133, 1304
ScrFI CCNGG 3 cut(s) 166, 547, 814
SfaNI GCATC 3 cut(s) 433, 1060, 1255
SfcI CTRYAG 2 cut(s) 528, 1202
SinI GGWCC 2 cut(s) 286, 729
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
SphI GCATGC 1 cut(s) 748
Sse9I AATT 7 cut(s) 143, 199, 913, 1001, 1101, 1275, 1320
SsiI CCGC 2 cut(s) 297, 550
SspMI CTAG 7 cut(s) 126, 215, 327, 351, 509, 663, 1023
StyD4I CCNGG 3 cut(s) 164, 545, 812
StyI CCWWGG 3 cut(s) 125, 508, 1150
TaaI ACNGT 4 cut(s) 178, 572, 991, 1223
TaiI ACGT 1 cut(s) 673
TaqI TCGA 2 cut(s) 454, 993
TasI AATT 7 cut(s) 143, 199, 913, 1001, 1101, 1275, 1320
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 5 cut(s) 76, 109, 347, 581, 1118
Tru1I TTAA 3 cut(s) 245, 362, 629
Tru9I TTAA 3 cut(s) 245, 362, 629
TscAI CASTG 2 cut(s) 453, 1017
TseFI GTSAC 2 cut(s) 156, 985
TseI GCWGC 6 cut(s) 495, 527, 530, 1241, 1359, 1362
Tsp45I GTSAC 2 cut(s) 156, 985
TspDTI ATGAA 6 cut(s) 35, 360, 813, 906, 1151, 1236
TspRI CASTG 2 cut(s) 453, 1017
VpaK11BI GGWCC 2 cut(s) 286, 729
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 2 cut(s) 913, 1275
XceI RCATGY 1 cut(s) 748
XmaJI CCTAGG 2 cut(s) 125, 508
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 7 cut(s) 126, 215, 327, 351, 509, 663, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.