FvH4_5g33060

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013179
Physical Location & Seq
Forward (+)
23878353 .. 23885631
7279 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33060.t2

Sequence Viewer

Length: 1032 bp
ATGAAACGCTGGCGCGCTCTCTACCGGAGTAGCTCGTTACGGCGGACCACCCCACACTCTCTCTCCTCCTCCACCGCTTTCTGTACCGACAGCCAAGCCGACGGACCTCAAACCTCCCGCTTCAAGGTCTTCGATCGCGACTTCCGACGCAAACAGCTTGATCGAGCGTCGTGGCTGATGCGTCCCAAAGACTCGTTCGTCGATTCCGTTGCTGACAACTTGTTAGATCGATTGGAGGATTGTAAGAAAACATTTCCAACAGCATTATGTTTAGGTGGTTCTTTGGAAGCCGTCAGGTGCTTGCTCCATGGTCGAGGTGCCATCGAAAAGCTCATTATGATGTCTGCATCATATGACATGGTAAAACGATGTAGAAATGCTGAGGATGAGGTACAGAATGAAAACATCGAAACATCTTTCATGGTCGGTGACGAAGAGTTTTTGCCCATTAAAGAAAGTTCTCTAGATCTGGTAATAAGTTGCTTGGGCCTTCATTGGACAAATGATCTTCCAGGAGCTATGATACAGTGCAGATTGGCATTAAAACCAGATGGCTTATTCTTAGCAGCAATTCTTGGCGGAGAAACCTTAAAAGAGCTGAGAATTGCATGCACTGTGGCACAAATGGAGCGTGAAGGAGGTATTAGTCCACGAATATCGCCTTTAGCACAAGTGCGGGATGCAGGAAATCTTTTGACGAGGGCAGGCTTTACCCTTCCAGGTGTTGATGTTGATGAATATGTAGTGAGATATCCAAGTGCTCTGGATCTCATAGAACATTTGCGTGGAATGGGTGAAACCAATGCCCTTCTACAAAGGAACCCTATGCTCAAGAGAGATACAGCCCTTGCAACTGCAGCAATTTATGATTCAATGTTTGCAGCAGATGATGGCACCATCCCTGCAACCTTTCAGGTTATTTATATGACTGGTTGGAAGGAGCATTCATCTCAGCCGAGGGCCAAAAGGAGGGGATCTGCAACTGTATCCTTCCAGGATATCCAGAAACAATTTGGAAGTGAGACTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

344

Amino Acids

38.23

Weight (kDa)

6.96

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 98 - 188 9.1e-10 Methyltransferase domain
Methyltransf_25 PF13649 109 - 185 3.8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22800
fragaria_vesca FvH4_5g33060
malus_domestica MD04G1240000.v1.1 MD08G1215200.v1.1 MD15G1399100.v1.1
prunus_persica Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1
pyrus_communis pycom08g18630 pycom15g35810
rosa_chinensis RchiOBHm_Chr7g0231231
rosa_laevigata RLG00000001427
rosa_multiflora Rmu_sc0004571.1_g000020
rosa_roxburghii Rroxscaffold_3G00229420
rosa_rugosa Rorug07G0265200
rosa_samantha Rh7BG394200 Rh7CG438300
rosa_wichuraiana Rw7G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AccB1I GGYRCC 2 cut(s) 317, 893
AccII CGCG 2 cut(s) 15, 138
AciI CCGC 5 cut(s) 43, 75, 118, 579, 676
AclWI GGATC 2 cut(s) 774, 982
AfaI GTAC 2 cut(s) 85, 393
AfiI CCNNNNNNNGG 2 cut(s) 124, 969
AgsI TTSAA 2 cut(s) 124, 873
AhlI ACTAGT 1 cut(s) 1025
AjnI CCWGG 3 cut(s) 511, 718, 993
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 33, 157, 331, 518, 598
AluI AGCT 5 cut(s) 33, 157, 331, 518, 598
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 1 cut(s) 1016
AlwI GGATC 2 cut(s) 774, 982
AoxI GGCC 2 cut(s) 487, 960
ApeKI GCWGC 3 cut(s) 566, 857, 881
AspLEI GCGC 2 cut(s) 15, 17
AspS9I GGNCC 4 cut(s) 45, 104, 487, 960
AsuHPI GGTGA 2 cut(s) 440, 806
AvaII GGWCC 2 cut(s) 45, 104
BanI GGYRCC 2 cut(s) 317, 893
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 1 cut(s) 763
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 578, 869, 893
BccI CCATC 4 cut(s) 329, 545, 884, 905
BceAI ACGGC 2 cut(s) 56, 275
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 3 cut(s) 513, 720, 995
BciVI GTATCC 1 cut(s) 997
BcoDI GTCTC 1 cut(s) 1016
BcuI ACTAGT 1 cut(s) 1025
BfaI CTAG 2 cut(s) 464, 1026
BfmI CTRYAG 1 cut(s) 855
BfuI GTATCC 1 cut(s) 997
BglII AGATCT 1 cut(s) 466
BisI GCNGC 3 cut(s) 567, 858, 882
BlsI GCNGC 3 cut(s) 568, 859, 883
Bme1390I CCNGG 3 cut(s) 513, 720, 995
Bme18I GGWCC 2 cut(s) 45, 104
BmgT120I GGNCC 4 cut(s) 45, 104, 487, 960
BmiI GGNNCC 3 cut(s) 319, 821, 895
BmrFI CCNGG 3 cut(s) 513, 720, 995
BmsI GCATC 3 cut(s) 168, 356, 670
BpiI GAAGAC 1 cut(s) 121
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 815
Bsa29I ATCGAT 1 cut(s) 229
BsaJI CCNNGG 2 cut(s) 307, 956
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 969
Bse1I ACTGG 1 cut(s) 934
BseBI CCWGG 3 cut(s) 513, 720, 995
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 2 cut(s) 307, 956
BseGI GGATG 3 cut(s) 391, 685, 897
BseLI CCNNNNNNNGG 2 cut(s) 124, 969
BseMII CTCAG 3 cut(s) 372, 590, 965
BseNI ACTGG 1 cut(s) 934
BsePI GCGCGC 1 cut(s) 13
BseRI GAGGAG 2 cut(s) 55, 58
BseXI GCAGC 3 cut(s) 578, 869, 893
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 2 cut(s) 15, 138
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 2 cut(s) 489, 962
BshNI GGYRCC 2 cut(s) 317, 893
BshVI ATCGAT 1 cut(s) 229
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 763
BsiSI CCGG 1 cut(s) 25
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 2 cut(s) 124, 969
BsmAI GTCTC 1 cut(s) 1016
BsmFI GGGAC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 943
BsnI GGCC 2 cut(s) 489, 962
Bsp1286I GDGCHC 1 cut(s) 763
Bsp143I GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
Bsp19I CCATGG 1 cut(s) 307
Bsp68I TCGCGA 1 cut(s) 138
BspACI CCGC 5 cut(s) 43, 75, 118, 579, 676
BspANI GGCC 2 cut(s) 489, 962
BspCNI CTCAG 3 cut(s) 373, 591, 964
BspDI ATCGAT 1 cut(s) 229
BspFNI CGCG 2 cut(s) 15, 138
BspLI GGNNCC 3 cut(s) 319, 821, 895
BspMAI CTGCAG 1 cut(s) 859
BspPI GGATC 2 cut(s) 774, 982
BspT107I GGYRCC 2 cut(s) 317, 893
BsrI ACTGG 1 cut(s) 934
BssECI CCNNGG 2 cut(s) 307, 956
BssHII GCGCGC 1 cut(s) 13
BssMI GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 513, 720, 995
Bst4CI ACNGT 3 cut(s) 528, 616, 985
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 5 cut(s) 11, 15, 302, 610, 706
BstDEI CTNAG 4 cut(s) 381, 562, 599, 951
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 3 cut(s) 391, 685, 897
BstFNI CGCG 2 cut(s) 15, 138
BstHHI GCGC 2 cut(s) 15, 17
BstKTI GATC 7 cut(s) 136, 163, 229, 469, 508, 769, 977
BstMAI GTCTC 1 cut(s) 1016
BstMBI GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 1 cut(s) 857
BstNI CCWGG 3 cut(s) 513, 720, 995
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 3 cut(s) 511, 718, 993
BstSFI CTRYAG 1 cut(s) 855
BstUI CGCG 2 cut(s) 15, 138
BstV1I GCAGC 3 cut(s) 578, 869, 893
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 3 cut(s) 466, 766, 974
BstYI RGATCY 3 cut(s) 466, 766, 974
Bsu15I ATCGAT 1 cut(s) 229
BsuI GTATCC 1 cut(s) 997
BsuRI GGCC 2 cut(s) 489, 962
BsuTUI ATCGAT 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 3 cut(s) 391, 685, 897
BtsIMutI CAGTG 2 cut(s) 533, 612
BtuMI TCGCGA 1 cut(s) 138
Cac8I GCNNGC 5 cut(s) 11, 15, 302, 610, 706
CfoI GCGC 2 cut(s) 15, 17
Cfr13I GGNCC 4 cut(s) 45, 104, 487, 960
ClaI ATCGAT 1 cut(s) 229
CseI GACGC 3 cut(s) 156, 156, 170
Csp6I GTAC 2 cut(s) 84, 392
CviAII CATG 4 cut(s) 308, 358, 421, 609
CviQI GTAC 2 cut(s) 84, 392
DdeI CTNAG 4 cut(s) 381, 562, 599, 951
DpnI GATC 7 cut(s) 135, 162, 228, 468, 507, 768, 976
DpnII GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
EciI GGCGGA 2 cut(s) 58, 594
Eco130I CCWWGG 1 cut(s) 307
Eco32I GATATC 2 cut(s) 752, 1000
Eco47I GGWCC 2 cut(s) 45, 104
EcoRII CCWGG 3 cut(s) 511, 718, 993
EcoRV GATATC 2 cut(s) 752, 1000
EcoT14I CCWWGG 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 4 cut(s) 311, 361, 424, 612
FaqI GGGAC 1 cut(s) 168
FatI CATG 4 cut(s) 307, 357, 420, 608
FauI CCCGC 2 cut(s) 125, 669
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 567, 858, 882
FokI GGATG 3 cut(s) 398, 692, 884
Fsp4HI GCNGC 3 cut(s) 567, 858, 882
FspBI CTAG 2 cut(s) 464, 1026
GlaI GCGC 2 cut(s) 14, 16
GluI GCNGC 3 cut(s) 567, 858, 882
HaeIII GGCC 2 cut(s) 489, 962
HapII CCGG 1 cut(s) 25
HgaI GACGC 3 cut(s) 156, 156, 170
HhaI GCGC 2 cut(s) 15, 17
Hin1II CATG 4 cut(s) 311, 361, 424, 612
Hin6I GCGC 2 cut(s) 13, 15
HinP1I GCGC 2 cut(s) 13, 15
HinfI GANTC 3 cut(s) 191, 203, 869
HpaII CCGG 1 cut(s) 25
HphI GGTGA 2 cut(s) 440, 806
Hpy166II GTNNAC 1 cut(s) 650
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 5 cut(s) 137, 464, 764, 832, 1003
Hpy8I GTNNAC 1 cut(s) 650
Hpy99I CGWCG 4 cut(s) 104, 150, 172, 203
HpyAV CCTTC 6 cut(s) 500, 629, 725, 818, 931, 1000
HpyCH4III ACNGT 3 cut(s) 528, 616, 985
HpyF10VI GCNNNNNNNGC 1 cut(s) 857
HpyF3I CTNAG 4 cut(s) 381, 562, 599, 951
Hsp92II CATG 4 cut(s) 311, 361, 424, 612
HspAI GCGC 2 cut(s) 13, 15
Kzo9I GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
LmnI GCTCC 4 cut(s) 309, 515, 628, 940
Lsp1109I GCAGC 3 cut(s) 578, 869, 893
LweI GCATC 3 cut(s) 168, 356, 670
MaeI CTAG 2 cut(s) 464, 1026
MaeIII GTNAC 2 cut(s) 36, 428
MalI GATC 7 cut(s) 135, 162, 228, 468, 507, 768, 976
MboI GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
MboII GAAGA 3 cut(s) 121, 446, 500
MflI RGATCY 3 cut(s) 466, 766, 974
MhlI GDGCHC 1 cut(s) 763
MluCI AATT 4 cut(s) 570, 603, 861, 1010
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 3 cut(s) 169, 281, 914
MseI TTAA 3 cut(s) 450, 542, 590
MslI CAYNNNNRTG 2 cut(s) 338, 783
MspI CCGG 1 cut(s) 25
MspR9I CCNGG 3 cut(s) 513, 720, 995
Mva1269I GAATGC 1 cut(s) 943
MvaI CCWGG 3 cut(s) 513, 720, 995
MvnI CGCG 2 cut(s) 15, 138
MwoI GCNNNNNNNGC 1 cut(s) 857
NcoI CCATGG 1 cut(s) 307
NdeI CATATG 1 cut(s) 352
NdeII GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
NlaIII CATG 4 cut(s) 311, 361, 424, 612
NlaIV GGNNCC 3 cut(s) 319, 821, 895
NmeAIII GCCGAG 1 cut(s) 981
NmuCI GTSAC 1 cut(s) 428
NruI TCGCGA 1 cut(s) 138
NspI RCATGY 1 cut(s) 612
PaeI GCATGC 1 cut(s) 612
PauI GCGCGC 1 cut(s) 13
PcsI WCGNNNNNNNCGW 2 cut(s) 142, 204
PctI GAATGC 1 cut(s) 943
PfeI GAWTC 2 cut(s) 203, 869
PfoI TCCNGGA 2 cut(s) 511, 993
PkrI GCNGC 3 cut(s) 568, 859, 883
Ple19I CGATCG 1 cut(s) 136
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Psp6I CCWGG 3 cut(s) 511, 718, 993
PspGI CCWGG 3 cut(s) 511, 718, 993
PspN4I GGNNCC 3 cut(s) 319, 821, 895
PspPI GGNCC 4 cut(s) 45, 104, 487, 960
PstI CTGCAG 1 cut(s) 859
PsuI RGATCY 3 cut(s) 466, 766, 974
PteI GCGCGC 1 cut(s) 13
PvuI CGATCG 1 cut(s) 136
RruI TCGCGA 1 cut(s) 138
RsaI GTAC 2 cut(s) 85, 393
RsaNI GTAC 2 cut(s) 84, 392
RseI CAYNNNNRTG 2 cut(s) 338, 783
SaqAI TTAA 3 cut(s) 450, 542, 590
SatI GCNGC 3 cut(s) 567, 858, 882
Sau3AI GATC 7 cut(s) 133, 160, 226, 466, 505, 766, 974
Sau96I GGNCC 4 cut(s) 45, 104, 487, 960
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 513, 720, 995
SduI GDGCHC 1 cut(s) 763
SfaNI GCATC 3 cut(s) 168, 356, 670
SfcI CTRYAG 1 cut(s) 855
SinI GGWCC 2 cut(s) 45, 104
SmiMI CAYNNNNRTG 2 cut(s) 338, 783
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
SpeI ACTAGT 1 cut(s) 1025
SphI GCATGC 1 cut(s) 612
Sse9I AATT 4 cut(s) 570, 603, 861, 1010
SsiI CCGC 5 cut(s) 43, 75, 118, 579, 676
SspMI CTAG 2 cut(s) 464, 1026
StyD4I CCNGG 3 cut(s) 511, 718, 993
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 3 cut(s) 528, 616, 985
TaqI TCGA 7 cut(s) 132, 163, 201, 229, 313, 324, 408
TasI AATT 4 cut(s) 570, 603, 861, 1010
TfiI GAWTC 2 cut(s) 203, 869
Tru1I TTAA 3 cut(s) 450, 542, 590
Tru9I TTAA 3 cut(s) 450, 542, 590
TscAI CASTG 2 cut(s) 533, 619
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 3 cut(s) 566, 857, 881
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 6 cut(s) 17, 409, 414, 482, 750, 936
TspGWI ACGGA 2 cut(s) 117, 196
TspRI CASTG 2 cut(s) 533, 619
VpaK11BI GGWCC 2 cut(s) 45, 104
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 1 cut(s) 612
XcmI CCANNNNNNNNNTGG 1 cut(s) 1010
XspI CTAG 2 cut(s) 464, 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.