MD04G1240000.v1.1

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013179
Physical Location & Seq
Reverse (-)
31787040 .. 31788071
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1240000.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGAGAGGCTTGTACCGGAGGAGCTCGCTATGGAGGACAAGAACAGAAGCTCCGCACTCACTCTTCTTCTTCTCAAATGCTCTCTGTACCGACGCCAACATTGATGGGGTTCAAAGCTCCAGGATCAAGGTCTTCGATCGCCACTTCAGACGCAAACAGCTTGATCGAGCTGCTTGGTTGATGCGTTCCAAGGATTCTTTTGTTGATACTGTGGCTAAGAATCTATTGGATCGTTTGGAGGACTGCAAGAAAACATTTCCAGCGGCATTATGTTTAGGAGGTCAATTGGAAGCTGTCAGGCGCTTGTTACGTGGTCGTGGTGCCATTGAAAAGCTCATTATGATGTCTGCATCATCTGACATGGTAGAACTGTGTAGAAATGCTGAGCGTGAGTTACAAACTGAAAACATTGAAACATCCTTTATGGTTGGTGACGAAGAGTTTTTGCCCGTTAAGGAAAATTCTGTGGATCTGGTATTAAGCTGCTTGGGACTTCACTGGACAAATGATCTTCCAGGAGCCATGATACAGTGCAGATCGGCATTGAAGCCAGATGGCCTATTTTTAGCAGCGATTCTGGGTGGAGAAACCTTAAAAGAGCTGAGAATAGCGTGCACCGTGGCACAAATGGAGCGTGAAGGAGGCATCAGTCCACGAATATCACCTTTGGCGCAAGTGCGGGATGCAGGCAATCTTTTGACCAGGGCAGGCTTTACCCTTCCAGGTGTTGATGTGGACGAATTTGTAGTAAGATATCCAAGTGCTCTGGATCTTATAGAACATTTGCGTGCAATGGGTGAAACCAATGCTCTGCTACAAAGGAACACTATTTTAAAGAGAGAAACTGCCCTGGCCACCGCAGCAATTTATGATTCTATGTTTGCAGCAGAAGATGGCACCATCCCAGCAACCTTCCAGGTGATTTATATGACAGGGTGGAGGGACCATCCATCTCAGCAGAGGGCAAAGAGGAGGGGGTCGGCAACTGTATCTTTTCAGGACATCCAGAACCAATTTGGAAGTGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

344

Amino Acids

38.39

Weight (kDa)

6.97

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 100 - 189 2.4e-11 Methyltransferase domain
Methyltransf_25 PF13649 109 - 186 3.6e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22800
fragaria_vesca FvH4_5g33060
malus_domestica MD04G1240000.v1.1 MD08G1215200.v1.1 MD15G1399100.v1.1
prunus_persica Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1
pyrus_communis pycom08g18630 pycom15g35810
rosa_chinensis RchiOBHm_Chr7g0231231
rosa_laevigata RLG00000001427
rosa_multiflora Rmu_sc0004571.1_g000020
rosa_roxburghii Rroxscaffold_3G00229420
rosa_rugosa Rorug07G0265200
rosa_samantha Rh7BG394200 Rh7CG438300
rosa_wichuraiana Rw7G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 320, 896
AciI CCGC 4 cut(s) 53, 263, 679, 858
AclWI GGATC 4 cut(s) 131, 237, 477, 777
AcoI YGGCCR 1 cut(s) 852
AcsI RAATTY 2 cut(s) 460, 740
AcuI CTGAAG 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 14, 88
AgsI TTSAA 4 cut(s) 113, 329, 413, 547
AjnI CCWGG 6 cut(s) 119, 514, 701, 721, 849, 915
AloI GAACNNNNNNTCC 2 cut(s) 34, 66
AluBI AGCT 9 cut(s) 24, 50, 117, 160, 170, 293, 334, 483, 601
AluI AGCT 9 cut(s) 24, 50, 117, 160, 170, 293, 334, 483, 601
Alw21I GWGCWC 3 cut(s) 26, 617, 766
Alw44I GTGCAC 1 cut(s) 613
AlwI GGATC 4 cut(s) 131, 237, 477, 777
AoxI GGCC 2 cut(s) 556, 852
ApaLI GTGCAC 1 cut(s) 613
ApeKI GCWGC 5 cut(s) 170, 483, 569, 860, 884
ApoI RAATTY 2 cut(s) 460, 740
AspLEI GCGC 2 cut(s) 303, 673
AspS9I GGNCC 1 cut(s) 943
AsuHPI GGTGA 4 cut(s) 443, 654, 809, 931
AvaII GGWCC 1 cut(s) 943
BaeGI GKGCMC 1 cut(s) 617
BalI TGGCCA 1 cut(s) 854
BanI GGYRCC 2 cut(s) 320, 896
BanII GRGCYC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 3 cut(s) 26, 617, 766
BbvI GCAGC 5 cut(s) 157, 470, 581, 872, 896
BccI CCATC 6 cut(s) 98, 548, 887, 908, 954, 958
BciT130I CCWGG 6 cut(s) 121, 516, 703, 723, 851, 917
BfoI RGCGCY 1 cut(s) 304
BisI GCNGC 6 cut(s) 171, 264, 484, 570, 861, 885
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 6 cut(s) 172, 265, 485, 571, 862, 886
Bme1390I CCNGG 6 cut(s) 121, 516, 703, 723, 851, 917
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 4 cut(s) 322, 520, 898, 944
BmrFI CCNGG 6 cut(s) 121, 516, 703, 723, 851, 917
BmsI GCATC 4 cut(s) 171, 359, 654, 673
BpiI GAAGAC 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 103
Bpu1102I GCTNAGC 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 4 cut(s) 189, 618, 702, 849
BsaWI WCCGGW 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bse1I ACTGG 1 cut(s) 503
Bse3DI GCAATG 1 cut(s) 798
BseBI CCWGG 6 cut(s) 121, 516, 703, 723, 851, 917
BseDI CCNNGG 4 cut(s) 189, 618, 702, 849
BseGI GGATG 5 cut(s) 416, 688, 900, 946, 1002
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 3 cut(s) 375, 593, 968
BseNI ACTGG 1 cut(s) 503
BseRI GAGGAG 2 cut(s) 34, 985
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 5 cut(s) 157, 470, 581, 872, 896
BseYI CCCAGC 1 cut(s) 904
BsgI GTGCAG 1 cut(s) 553
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 2 cut(s) 558, 854
BshNI GGYRCC 2 cut(s) 320, 896
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 3 cut(s) 26, 617, 766
BsiSI CCGG 1 cut(s) 16
BslFI GGGAC 2 cut(s) 504, 956
BsmFI GGGAC 2 cut(s) 504, 956
BsnI GGCC 2 cut(s) 558, 854
Bsp1286I GDGCHC 3 cut(s) 26, 617, 766
Bsp143I GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 4 cut(s) 53, 263, 679, 858
BspANI GGCC 2 cut(s) 558, 854
BspCNI CTCAG 3 cut(s) 376, 594, 967
BspLI GGNNCC 4 cut(s) 322, 520, 898, 944
BspPI GGATC 4 cut(s) 131, 237, 477, 777
BspT107I GGYRCC 2 cut(s) 320, 896
BsrDI GCAATG 1 cut(s) 798
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 4 cut(s) 189, 618, 702, 849
BssMI GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 6 cut(s) 121, 516, 703, 723, 851, 917
Bst4CI ACNGT 5 cut(s) 211, 372, 531, 619, 988
Bst6I CTCTTC 2 cut(s) 68, 432
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 5 cut(s) 26, 613, 688, 709, 789
BstDEI CTNAG 4 cut(s) 216, 384, 602, 954
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 5 cut(s) 416, 688, 900, 946, 1002
BstH2I RGCGCY 1 cut(s) 304
BstHHI GCGC 2 cut(s) 303, 673
BstKTI GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 539, 772
BstMBI GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 860
BstNI CCWGG 6 cut(s) 121, 516, 703, 723, 851, 917
BstSCI CCNGG 6 cut(s) 119, 514, 701, 721, 849, 915
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 5 cut(s) 157, 470, 581, 872, 896
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 2 cut(s) 469, 769
BstYI RGATCY 2 cut(s) 469, 769
BsuRI GGCC 2 cut(s) 558, 854
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 5 cut(s) 416, 688, 900, 946, 1002
BtsIMutI CAGTG 2 cut(s) 496, 536
Cac8I GCNNGC 5 cut(s) 26, 613, 688, 709, 789
CfoI GCGC 2 cut(s) 303, 673
Cfr13I GGNCC 1 cut(s) 943
CseI GACGC 2 cut(s) 101, 159
Csp6I GTAC 2 cut(s) 13, 87
CviAII CATG 2 cut(s) 361, 523
CviQI GTAC 2 cut(s) 13, 87
DdeI CTNAG 4 cut(s) 216, 384, 602, 954
DpnI GATC 8 cut(s) 125, 138, 165, 231, 471, 510, 538, 771
DpnII GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
DraI TTTAAA 1 cut(s) 834
EaeI YGGCCR 1 cut(s) 852
Eam1104I CTCTTC 2 cut(s) 68, 432
EarI CTCTTC 2 cut(s) 68, 432
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 26
Eco32I GATATC 1 cut(s) 755
Eco47I GGWCC 1 cut(s) 943
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 24
EcoRII CCWGG 6 cut(s) 119, 514, 701, 721, 849, 915
EcoRV GATATC 1 cut(s) 755
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 2 cut(s) 364, 526
FaqI GGGAC 2 cut(s) 504, 956
FatI CATG 2 cut(s) 360, 522
FauI CCCGC 1 cut(s) 672
Fnu4HI GCNGC 6 cut(s) 171, 264, 484, 570, 861, 885
FokI GGATG 5 cut(s) 403, 695, 887, 933, 989
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 6 cut(s) 171, 264, 484, 570, 861, 885
GlaI GCGC 2 cut(s) 302, 672
GluI GCNGC 6 cut(s) 171, 264, 484, 570, 861, 885
GsaI CCCAGC 1 cut(s) 908
GsuI CTGGAG 1 cut(s) 103
HaeII RGCGCY 1 cut(s) 304
HaeIII GGCC 2 cut(s) 558, 854
HapII CCGG 1 cut(s) 16
HgaI GACGC 2 cut(s) 101, 159
HhaI GCGC 2 cut(s) 303, 673
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 2 cut(s) 364, 526
Hin6I GCGC 2 cut(s) 301, 671
HinP1I GCGC 2 cut(s) 301, 671
HinfI GANTC 4 cut(s) 194, 220, 574, 872
HpaII CCGG 1 cut(s) 16
HphI GGTGA 4 cut(s) 443, 654, 809, 931
Hpy166II GTNNAC 3 cut(s) 615, 653, 736
Hpy188I TCNGA 2 cut(s) 149, 358
Hpy188III TCNNGA 3 cut(s) 767, 998, 1006
Hpy8I GTNNAC 3 cut(s) 615, 653, 736
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 3 cut(s) 632, 728, 922
HpyCH4III ACNGT 5 cut(s) 211, 372, 531, 619, 988
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 7 cut(s) 246, 350, 534, 615, 686, 791, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 860
HpyF3I CTNAG 4 cut(s) 216, 384, 602, 954
HpySE526I ACGT 1 cut(s) 310
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 2 cut(s) 364, 526
HspAI GCGC 2 cut(s) 301, 671
Kzo9I GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
LmnI GCTCC 5 cut(s) 21, 55, 122, 518, 631
Lsp1109I GCAGC 5 cut(s) 157, 470, 581, 872, 896
LweI GCATC 4 cut(s) 171, 359, 654, 673
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 3 cut(s) 306, 393, 431
MalI GATC 8 cut(s) 125, 138, 165, 231, 471, 510, 538, 771
MboI GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
MboII GAAGA 7 cut(s) 55, 58, 61, 124, 449, 503, 902
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 2 cut(s) 469, 769
MhlI GDGCHC 3 cut(s) 26, 617, 766
MlsI TGGCCA 1 cut(s) 854
MluCI AATT 5 cut(s) 284, 460, 740, 864, 1013
MluNI TGGCCA 1 cut(s) 854
Mox20I TGGCCA 1 cut(s) 854
MscI TGGCCA 1 cut(s) 854
MseI TTAA 4 cut(s) 453, 479, 593, 833
MslI CAYNNNNRTG 2 cut(s) 341, 786
Msp20I TGGCCA 1 cut(s) 854
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 6 cut(s) 121, 516, 703, 723, 851, 917
MunI CAATTG 1 cut(s) 284
MvaI CCWGG 6 cut(s) 121, 516, 703, 723, 851, 917
MwoI GCNNNNNNNGC 1 cut(s) 860
NdeII GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
NlaIII CATG 2 cut(s) 364, 526
NlaIV GGNNCC 4 cut(s) 322, 520, 898, 944
NmuCI GTSAC 1 cut(s) 431
PfeI GAWTC 4 cut(s) 194, 220, 574, 872
PfoI TCCNGGA 2 cut(s) 119, 514
PkrI GCNGC 6 cut(s) 172, 265, 485, 571, 862, 886
Ple19I CGATCG 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 26
Psp6I CCWGG 6 cut(s) 119, 514, 701, 721, 849, 915
PspFI CCCAGC 1 cut(s) 904
PspGI CCWGG 6 cut(s) 119, 514, 701, 721, 849, 915
PspN4I GGNNCC 4 cut(s) 322, 520, 898, 944
PspPI GGNCC 1 cut(s) 943
PsuI RGATCY 2 cut(s) 469, 769
PvuI CGATCG 1 cut(s) 139
RsaI GTAC 2 cut(s) 14, 88
RsaNI GTAC 2 cut(s) 13, 87
RseI CAYNNNNRTG 2 cut(s) 341, 786
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 4 cut(s) 453, 479, 593, 833
SatI GCNGC 6 cut(s) 171, 264, 484, 570, 861, 885
Sau3AI GATC 8 cut(s) 123, 136, 163, 229, 469, 508, 536, 769
Sau96I GGNCC 1 cut(s) 943
ScrFI CCNGG 6 cut(s) 121, 516, 703, 723, 851, 917
SduI GDGCHC 3 cut(s) 26, 617, 766
SfaNI GCATC 4 cut(s) 171, 359, 654, 673
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 2 cut(s) 341, 786
Sse9I AATT 5 cut(s) 284, 460, 740, 864, 1013
SsiI CCGC 4 cut(s) 53, 263, 679, 858
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 6 cut(s) 119, 514, 701, 721, 849, 915
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 5 cut(s) 211, 372, 531, 619, 988
TaiI ACGT 1 cut(s) 313
TaqI TCGA 2 cut(s) 135, 166
TasI AATT 5 cut(s) 284, 460, 740, 864, 1013
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 4 cut(s) 194, 220, 574, 872
Tru1I TTAA 4 cut(s) 453, 479, 593, 833
Tru9I TTAA 4 cut(s) 453, 479, 593, 833
TscAI CASTG 2 cut(s) 503, 536
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 5 cut(s) 170, 483, 569, 860, 884
Tsp45I GTSAC 1 cut(s) 431
TspRI CASTG 2 cut(s) 503, 536
VneI GTGCAC 1 cut(s) 613
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 2 cut(s) 460, 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.