MD15G1399100.v1.1

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013179
Physical Location & Seq
Reverse (-)
50002076 .. 50008084
6009 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1399100.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGAGAGCCTTGTACCGGAGGTGCTCGCTATGGAGGACAAGAAGAGAAGCTCCGCACTCACTTTTCTTCTTCTCAAATGCTCTCTGTACCCACACCAACAACATTGATGGGGTTCACAACTCCAGGATCAAGGTCTTTGATCGCCACTTCAGACGCAAACAGCTTGATCGAGCTGCTTGGTTGATGCGTTCCAAGGATTCTTTTGTTGATACTGTTGCTGAGAATCTATTAGATCGCTTGGAGGACTGCAAGAAAACTTTTCCAACAGCATTATGTTTGGGAGGCCAATTAGAAGCTGTCAGGCGTTTGTTAAATGGTCGCGGTGCCATTGAAAAGCTCATTATGATGTCGGCATCATATGACATGGCAAAACTGTGTAGAAATGCTGAGCATGAGTTACAAACTGAAAACATTGAAACATCCTTTATGGTTGGTGACGAAGAGTTTTTGCCGGTCAAGGAAAGTTCTGTGGATCTGGTATTAAGTTGCTTGGGCCTTCACTGGACAAATGATCTTCCAGGAGCAATGATACAGTGCAGATTGGCATTGAAGCCAGATGGCCTATTTTTAGCGGCAATTCTTGGTGGAGAAACCTTAAAGTTAGAACTGAGGATAGCATGCACCGTGGCACAAATGGAGCGTGAAGGAGGCATCAGTCCACGAGTATCACCTTTGGCACAAGTGCGGGATGCAGGCAATCTCTTGACCAGGGCAGGCTTTACCCTTCCAGGTGTTGATGTGGATGAGTTTGTAGTAAGATATCCAAGCGCTCTGGATCTCATAGAACATCTGCGTGCAATGGGTGAAACGAATGCTCTTCTACAAAGGAACACTATTTTAAAGAGAGACACGGCCCTGGCCACTGCAGCAATTTATGATTCAATGTTTGCAGCAGAAGATGGCACCATCCCAGCAACCTTCCAGGTGATTTATATGACAGGGTGGAGTGACCATCCATCTCAGCAGAGGGCAAAGAGGAGGGGATCTGCAACTGTATCTTTTCAGGACATCCAGAAGCAATTTGGAAGTGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

346

Amino Acids

38.71

Weight (kDa)

7.62

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 103 - 190 1.5e-09 Methyltransferase domain
Methyltransf_25 PF13649 114 - 187 7.4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22800
fragaria_vesca FvH4_5g33060
malus_domestica MD04G1240000.v1.1 MD08G1215200.v1.1 MD15G1399100.v1.1
prunus_persica Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1
pyrus_communis pycom08g18630 pycom15g35810
rosa_chinensis RchiOBHm_Chr7g0231231
rosa_laevigata RLG00000001427
rosa_multiflora Rmu_sc0004571.1_g000020
rosa_roxburghii Rroxscaffold_3G00229420
rosa_rugosa Rorug07G0265200
rosa_samantha Rh7BG394200 Rh7CG438300
rosa_wichuraiana Rw7G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 323, 902
AccII CGCG 1 cut(s) 321
AciI CCGC 4 cut(s) 53, 321, 572, 685
AclWI GGATC 4 cut(s) 134, 480, 783, 991
AcoI YGGCCR 1 cut(s) 858
AcuI CTGAAG 1 cut(s) 133
AfaI GTAC 2 cut(s) 14, 88
AfeI AGCGCT 1 cut(s) 769
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 4 cut(s) 332, 416, 550, 882
AjnI CCWGG 6 cut(s) 122, 517, 707, 727, 855, 921
AluBI AGCT 5 cut(s) 50, 163, 173, 296, 337
AluI AGCT 5 cut(s) 50, 163, 173, 296, 337
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 2 cut(s) 840, 1025
AlwI GGATC 4 cut(s) 134, 480, 783, 991
Aor51HI AGCGCT 1 cut(s) 769
AoxI GGCC 5 cut(s) 283, 493, 559, 852, 858
ApeKI GCWGC 3 cut(s) 173, 866, 890
AspLEI GCGC 1 cut(s) 770
AspS9I GGNCC 2 cut(s) 493, 853
AsuHPI GGTGA 4 cut(s) 446, 660, 815, 937
BalI TGGCCA 1 cut(s) 860
BanI GGYRCC 2 cut(s) 323, 902
BauI CACGAG 1 cut(s) 660
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 3 cut(s) 160, 878, 902
BccI CCATC 6 cut(s) 101, 551, 893, 914, 960, 964
BceAI ACGGC 1 cut(s) 867
BciT130I CCWGG 6 cut(s) 124, 519, 709, 729, 857, 923
BcoDI GTCTC 2 cut(s) 840, 1025
BfmI CTRYAG 1 cut(s) 864
BfoI RGCGCY 1 cut(s) 771
BisI GCNGC 4 cut(s) 174, 573, 867, 891
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 4 cut(s) 175, 574, 868, 892
Bme1390I CCNGG 6 cut(s) 124, 519, 709, 729, 857, 923
BmgT120I GGNCC 2 cut(s) 493, 853
BmiI GGNNCC 2 cut(s) 325, 904
BmrFI CCNGG 6 cut(s) 124, 519, 709, 729, 857, 923
BmsI GCATC 4 cut(s) 174, 362, 660, 679
BpmI CTGGAG 1 cut(s) 106
Bpu1102I GCTNAGC 1 cut(s) 387
BsaJI CCNNGG 4 cut(s) 192, 624, 708, 855
BsaWI WCCGGW 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 2 cut(s) 531, 804
BseBI CCWGG 6 cut(s) 124, 519, 709, 729, 857, 923
BseDI CCNNGG 4 cut(s) 192, 624, 708, 855
BseGI GGATG 6 cut(s) 419, 694, 748, 906, 952, 1008
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMI GCAATG 2 cut(s) 531, 804
BseMII CTCAG 4 cut(s) 210, 378, 599, 974
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 991
BseXI GCAGC 3 cut(s) 160, 878, 902
BseYI CCCAGC 1 cut(s) 910
BsgI GTGCAG 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 5 cut(s) 285, 495, 561, 854, 860
BshNI GGYRCC 2 cut(s) 323, 902
BsiHKAI GWGCWC 1 cut(s) 26
BsiSI CCGG 2 cut(s) 16, 452
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 2 cut(s) 840, 1025
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 5 cut(s) 285, 495, 561, 854, 860
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 4 cut(s) 53, 321, 572, 685
BspANI GGCC 5 cut(s) 285, 495, 561, 854, 860
BspCNI CTCAG 4 cut(s) 211, 379, 600, 973
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 2 cut(s) 325, 904
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 4 cut(s) 134, 480, 783, 991
BspQI GCTCTTC 1 cut(s) 822
BspT107I GGYRCC 2 cut(s) 323, 902
BsrDI GCAATG 2 cut(s) 531, 804
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 4 cut(s) 192, 624, 708, 855
BssMI GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 6 cut(s) 124, 519, 709, 729, 857, 923
Bst4CI ACNGT 5 cut(s) 214, 375, 534, 625, 994
Bst6I CTCTTC 3 cut(s) 37, 435, 822
BstC8I GCNNGC 5 cut(s) 26, 619, 694, 715, 795
BstDEI CTNAG 4 cut(s) 219, 387, 608, 960
BstDSI CCRYGG 1 cut(s) 624
BstF5I GGATG 6 cut(s) 419, 694, 748, 906, 952, 1008
BstFNI CGCG 1 cut(s) 321
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 1 cut(s) 770
BstKTI GATC 8 cut(s) 129, 142, 169, 235, 475, 514, 778, 986
BstMAI GTCTC 2 cut(s) 840, 1025
BstMBI GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
BstMWI GCNNNNNNNGC 1 cut(s) 866
BstNI CCWGG 6 cut(s) 124, 519, 709, 729, 857, 923
BstNSI RCATGY 1 cut(s) 621
BstSCI CCNGG 6 cut(s) 122, 517, 707, 727, 855, 921
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 3 cut(s) 160, 878, 902
BstX2I RGATCY 3 cut(s) 472, 775, 983
BstYI RGATCY 3 cut(s) 472, 775, 983
BsuRI GGCC 5 cut(s) 285, 495, 561, 854, 860
BtgI CCRYGG 1 cut(s) 624
BtsCI GGATG 6 cut(s) 419, 694, 748, 906, 952, 1008
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 3 cut(s) 499, 539, 861
Cac8I GCNNGC 5 cut(s) 26, 619, 694, 715, 795
CfoI GCGC 1 cut(s) 770
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 493, 853
CseI GACGC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 13, 87
CviAII CATG 3 cut(s) 364, 392, 618
CviQI GTAC 2 cut(s) 13, 87
DdeI CTNAG 4 cut(s) 219, 387, 608, 960
DpnI GATC 8 cut(s) 128, 141, 168, 234, 474, 513, 777, 985
DpnII GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
DraI TTTAAA 1 cut(s) 840
EaeI YGGCCR 1 cut(s) 858
Eam1104I CTCTTC 3 cut(s) 37, 435, 822
EarI CTCTTC 3 cut(s) 37, 435, 822
Eco130I CCWWGG 1 cut(s) 192
Eco32I GATATC 1 cut(s) 761
Eco47III AGCGCT 1 cut(s) 769
Eco57I CTGAAG 1 cut(s) 133
EcoRII CCWGG 6 cut(s) 122, 517, 707, 727, 855, 921
EcoRV GATATC 1 cut(s) 761
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 3 cut(s) 367, 395, 621
FalI AAGNNNNNCTT 1 cut(s) 1018
FatI CATG 3 cut(s) 363, 391, 617
FauI CCCGC 1 cut(s) 678
FauNDI CATATG 1 cut(s) 358
Fnu4HI GCNGC 4 cut(s) 174, 573, 867, 891
FokI GGATG 6 cut(s) 406, 701, 755, 893, 939, 995
Fsp4HI GCNGC 4 cut(s) 174, 573, 867, 891
GlaI GCGC 1 cut(s) 769
GluI GCNGC 4 cut(s) 174, 573, 867, 891
GsaI CCCAGC 1 cut(s) 914
GsuI CTGGAG 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 5 cut(s) 285, 495, 561, 854, 860
HapII CCGG 2 cut(s) 16, 452
HgaI GACGC 1 cut(s) 162
HhaI GCGC 1 cut(s) 770
Hin1II CATG 3 cut(s) 367, 395, 621
Hin6I GCGC 1 cut(s) 768
HinP1I GCGC 1 cut(s) 768
HinfI GANTC 3 cut(s) 197, 223, 878
HpaII CCGG 2 cut(s) 16, 452
HphI GGTGA 4 cut(s) 446, 660, 815, 937
Hpy166II GTNNAC 2 cut(s) 115, 659
Hpy188I TCNGA 1 cut(s) 152
Hpy188III TCNNGA 4 cut(s) 703, 773, 1004, 1012
Hpy8I GTNNAC 2 cut(s) 115, 659
HpyAV CCTTC 4 cut(s) 506, 638, 734, 928
HpyCH4III ACNGT 5 cut(s) 214, 375, 534, 625, 994
HpyCH4V TGCA 8 cut(s) 249, 537, 621, 692, 797, 866, 890, 989
HpyF10VI GCNNNNNNNGC 1 cut(s) 866
HpyF3I CTNAG 4 cut(s) 219, 387, 608, 960
Hsp92II CATG 3 cut(s) 367, 395, 621
HspAI GCGC 1 cut(s) 768
Kzo9I GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
LguI GCTCTTC 1 cut(s) 822
LmnI GCTCC 3 cut(s) 55, 521, 637
Lsp1109I GCAGC 3 cut(s) 160, 878, 902
LweI GCATC 4 cut(s) 174, 362, 660, 679
MaeIII GTNAC 3 cut(s) 396, 434, 947
MalI GATC 8 cut(s) 128, 141, 168, 234, 474, 513, 777, 985
MboI GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
MboII GAAGA 7 cut(s) 54, 58, 61, 452, 506, 809, 908
MflI RGATCY 3 cut(s) 472, 775, 983
MhlI GDGCHC 1 cut(s) 26
MlsI TGGCCA 1 cut(s) 860
MluCI AATT 4 cut(s) 287, 576, 870, 1019
MluNI TGGCCA 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 287
MnlI CCTC 9 cut(s) 12, 27, 235, 275, 603, 641, 960, 969, 972
Mox20I TGGCCA 1 cut(s) 860
MscI TGGCCA 1 cut(s) 860
MseI TTAA 4 cut(s) 311, 482, 596, 839
MslI CAYNNNNRTG 2 cut(s) 344, 792
Msp20I TGGCCA 1 cut(s) 860
MspI CCGG 2 cut(s) 16, 452
MspR9I CCNGG 6 cut(s) 124, 519, 709, 729, 857, 923
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 6 cut(s) 124, 519, 709, 729, 857, 923
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 866
NdeI CATATG 1 cut(s) 358
NdeII GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
NlaIII CATG 3 cut(s) 367, 395, 621
NlaIV GGNNCC 2 cut(s) 325, 904
NmuCI GTSAC 2 cut(s) 434, 947
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PciSI GCTCTTC 1 cut(s) 822
PctI GAATGC 1 cut(s) 817
PfeI GAWTC 3 cut(s) 197, 223, 878
PfoI TCCNGGA 2 cut(s) 122, 517
PkrI GCNGC 4 cut(s) 175, 574, 868, 892
Psp6I CCWGG 6 cut(s) 122, 517, 707, 727, 855, 921
PspFI CCCAGC 1 cut(s) 910
PspGI CCWGG 6 cut(s) 122, 517, 707, 727, 855, 921
PspN4I GGNNCC 2 cut(s) 325, 904
PspPI GGNCC 2 cut(s) 493, 853
PstI CTGCAG 1 cut(s) 868
PsuI RGATCY 3 cut(s) 472, 775, 983
RsaI GTAC 2 cut(s) 14, 88
RsaNI GTAC 2 cut(s) 13, 87
RseI CAYNNNNRTG 2 cut(s) 344, 792
SapI GCTCTTC 1 cut(s) 822
SaqAI TTAA 4 cut(s) 311, 482, 596, 839
SatI GCNGC 4 cut(s) 174, 573, 867, 891
Sau3AI GATC 8 cut(s) 126, 139, 166, 232, 472, 511, 775, 983
Sau96I GGNCC 2 cut(s) 493, 853
ScrFI CCNGG 6 cut(s) 124, 519, 709, 729, 857, 923
SduI GDGCHC 1 cut(s) 26
SfaNI GCATC 4 cut(s) 174, 362, 660, 679
SfcI CTRYAG 1 cut(s) 864
SmiMI CAYNNNNRTG 2 cut(s) 344, 792
SphI GCATGC 1 cut(s) 621
Sse9I AATT 4 cut(s) 287, 576, 870, 1019
SsiI CCGC 4 cut(s) 53, 321, 572, 685
StyD4I CCNGG 6 cut(s) 122, 517, 707, 727, 855, 921
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 5 cut(s) 214, 375, 534, 625, 994
TaqI TCGA 1 cut(s) 169
TasI AATT 4 cut(s) 287, 576, 870, 1019
TauI GCSGC 1 cut(s) 575
TfiI GAWTC 3 cut(s) 197, 223, 878
Tru1I TTAA 4 cut(s) 311, 482, 596, 839
Tru9I TTAA 4 cut(s) 311, 482, 596, 839
TscAI CASTG 3 cut(s) 506, 539, 868
TseFI GTSAC 2 cut(s) 434, 947
TseI GCWGC 3 cut(s) 173, 866, 890
Tsp45I GTSAC 2 cut(s) 434, 947
TspRI CASTG 3 cut(s) 506, 539, 868
XceI RCATGY 1 cut(s) 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.