Rroxscaffold_3G00229420

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013179
Physical Location & Seq
Reverse (-)
13892090 .. 13900314
8225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229420.1

Sequence Viewer

Length: 1047 bp
ATGAAGCCCTGGCGTTCTCTCTACCGGAGAAGCTCGTTATGGCGCACCACAACCATAGAATCATCCCCACACTCCATCTCCTTCTCCACCGCTTTCTGTACGGACAGCCACTCCGACGGAGTTCAAAGCTCCCGGTTCAAGGTCTTCGATCGCGATTTCCGACGCAAACAGCTTGATCGAGCTTCATGGCTGATGCGTTCCAAAGATTCCTTTGTTGATTCTGTTGCTGACAACCTATTAGATCGATTGGAGGATTGTAAGAAAACATTTCCAACAGCATTATGTTTAGGAGGTTCTTTGGAAGCTGTCAGGCGCTTGCTCCATGGTCGAGGTGCCATTGAAAAGCTCATTATGATGTCTACATCATATGACATGGTAAAACGATGTAGAAATGCTGAGGATGAGGTACAGAATGAAAACATTGAAACATCCTTCATGGTTGGCGATGAAGAGTTTTTGCCCATTAAAGAAAGTTCACTAGATCTGGTAATTAGTTGCTTGGGCCTTCATTGGACAAATGATCTTCCAGGAGCCATGATACAGTGCAGATTGGCATTAAAGCCAGATGGCTTATTTTTAGCAGCAATTCTGGGCGGGGAAACCTTAAAAGAGCTGAGAATTGCATGCACTGTGGCACAAATGGAGCGTGAAGGAGGCATCAGTCCACGAATATCGCCTCTGGCACAAGTGCGGGATGCAGGAAATCTTTTGACCAGGGCAGGCTTTACCCTTCCGGGTGTTGATGTTGATGAATATGTAGTGAGATATCCAAGTGCTCTGGATCTCATAGAACATCTGCGTGCAATGGGTGAAACCAATGCCCTTCTACAAAGGAACCCTATGCTCAAGAGAGATACAGCCCTTGCCACTGCAGCAATTTATGATTCAATGTTTGCAGCAGAAGATGGCACCATCCCTGCAACCTTTCAGGTTATTTATATGACTGGTTGGAGGGAGCATTCATCTCAGCCGAGGGCTAAAAGGAGGGGATCTGCAACCGTATCCTTCCAGGATATCCAGAAACAATTTGGAAGTGAGACTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

348

Amino Acids

39.0

Weight (kDa)

6.62

Isoelectric Point (pI)

53.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 102 - 193 5.9e-10 Methyltransferase domain
Methyltransf_25 PF13649 113 - 190 3.4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22800
fragaria_vesca FvH4_5g33060
malus_domestica MD04G1240000.v1.1 MD08G1215200.v1.1 MD15G1399100.v1.1
prunus_persica Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1
pyrus_communis pycom08g18630 pycom15g35810
rosa_chinensis RchiOBHm_Chr7g0231231
rosa_laevigata RLG00000001427
rosa_multiflora Rmu_sc0004571.1_g000020
rosa_roxburghii Rroxscaffold_3G00229420
rosa_rugosa Rorug07G0265200
rosa_samantha Rh7BG394200 Rh7CG438300
rosa_wichuraiana Rw7G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 332, 908
AccI GTMKAC 1 cut(s) 359
AccII CGCG 1 cut(s) 153
AciI CCGC 3 cut(s) 90, 594, 691
AclWI GGATC 2 cut(s) 789, 997
AfaI GTAC 2 cut(s) 100, 408
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 5 cut(s) 125, 139, 341, 425, 888
AhlI ACTAGT 1 cut(s) 1040
AjnI CCWGG 4 cut(s) 8, 526, 713, 1008
AluBI AGCT 7 cut(s) 33, 129, 172, 182, 305, 346, 613
AluI AGCT 7 cut(s) 33, 129, 172, 182, 305, 346, 613
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 1 cut(s) 1031
AlwI GGATC 2 cut(s) 789, 997
AoxI GGCC 1 cut(s) 502
ApeKI GCWGC 3 cut(s) 581, 872, 896
AspLEI GCGC 2 cut(s) 45, 315
AspS9I GGNCC 1 cut(s) 502
AsuC2I CCSGG 2 cut(s) 133, 735
AsuHPI GGTGA 1 cut(s) 821
BanI GGYRCC 2 cut(s) 332, 908
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 778
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 3 cut(s) 593, 884, 908
BccI CCATC 4 cut(s) 83, 560, 899, 920
BciT130I CCWGG 4 cut(s) 10, 528, 715, 1010
BciVI GTATCC 1 cut(s) 1012
BcnI CCSGG 2 cut(s) 133, 735
BcoDI GTCTC 1 cut(s) 1031
BcuI ACTAGT 1 cut(s) 1040
BfaI CTAG 2 cut(s) 479, 1041
BfmI CTRYAG 1 cut(s) 870
BfoI RGCGCY 1 cut(s) 316
BfuI GTATCC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 481
BisI GCNGC 3 cut(s) 582, 873, 897
BlsI GCNGC 3 cut(s) 583, 874, 898
Bme1390I CCNGG 6 cut(s) 10, 133, 528, 715, 735, 1010
BmgT120I GGNCC 1 cut(s) 502
BmiI GGNNCC 4 cut(s) 334, 532, 836, 910
BmrFI CCNGG 6 cut(s) 10, 133, 528, 715, 735, 1010
BmsI GCATC 3 cut(s) 183, 666, 685
BpiI GAAGAC 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 2 cut(s) 133, 735
Bsa29I ATCGAT 1 cut(s) 244
BsaJI CCNNGG 4 cut(s) 8, 322, 714, 971
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 949
Bse3DI GCAATG 1 cut(s) 810
BseBI CCWGG 4 cut(s) 10, 528, 715, 1010
BseCI ATCGAT 1 cut(s) 244
BseDI CCNNGG 4 cut(s) 8, 322, 714, 971
BseGI GGATG 5 cut(s) 62, 406, 428, 700, 912
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 3 cut(s) 387, 605, 980
BseNI ACTGG 1 cut(s) 949
BseXI GCAGC 3 cut(s) 593, 884, 908
BsgI GTGCAG 1 cut(s) 565
Bsh1236I CGCG 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 1 cut(s) 504
BshNI GGYRCC 2 cut(s) 332, 908
BshVI ATCGAT 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 778
BsiSI CCGG 3 cut(s) 25, 133, 734
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 1 cut(s) 1031
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 778
Bsp143I GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
Bsp19I CCATGG 1 cut(s) 322
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 3 cut(s) 90, 594, 691
BspANI GGCC 1 cut(s) 504
BspCNI CTCAG 3 cut(s) 388, 606, 979
BspDI ATCGAT 1 cut(s) 244
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 4 cut(s) 334, 532, 836, 910
BspMAI CTGCAG 1 cut(s) 874
BspPI GGATC 2 cut(s) 789, 997
BspT107I GGYRCC 2 cut(s) 332, 908
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 1 cut(s) 949
BssECI CCNNGG 4 cut(s) 8, 322, 714, 971
BssMI GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 4 cut(s) 10, 528, 715, 1010
Bst4CI ACNGT 3 cut(s) 543, 631, 1000
Bst6I CTCTTC 1 cut(s) 444
BstC8I GCNNGC 4 cut(s) 317, 625, 721, 801
BstDEI CTNAG 3 cut(s) 396, 614, 966
BstDSI CCRYGG 1 cut(s) 322
BstF5I GGATG 5 cut(s) 62, 406, 428, 700, 912
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 2 cut(s) 45, 315
BstKTI GATC 7 cut(s) 151, 178, 244, 484, 523, 784, 992
BstMAI GTCTC 1 cut(s) 1031
BstMBI GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 1 cut(s) 872
BstNI CCWGG 4 cut(s) 10, 528, 715, 1010
BstNSI RCATGY 1 cut(s) 627
BstSCI CCNGG 6 cut(s) 8, 131, 526, 713, 733, 1008
BstSFI CTRYAG 1 cut(s) 870
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 3 cut(s) 593, 884, 908
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 3 cut(s) 481, 781, 989
BstYI RGATCY 3 cut(s) 481, 781, 989
Bsu15I ATCGAT 1 cut(s) 244
BsuI GTATCC 1 cut(s) 1012
BsuRI GGCC 1 cut(s) 504
BsuTUI ATCGAT 1 cut(s) 244
BtgI CCRYGG 1 cut(s) 322
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 5 cut(s) 62, 406, 428, 700, 912
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 3 cut(s) 548, 627, 867
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 4 cut(s) 317, 625, 721, 801
CfoI GCGC 2 cut(s) 45, 315
Cfr13I GGNCC 1 cut(s) 502
ClaI ATCGAT 1 cut(s) 244
CseI GACGC 1 cut(s) 171
Csp6I GTAC 2 cut(s) 99, 407
CviAII CATG 6 cut(s) 186, 323, 373, 436, 535, 624
CviQI GTAC 2 cut(s) 99, 407
DdeI CTNAG 3 cut(s) 396, 614, 966
DpnI GATC 7 cut(s) 150, 177, 243, 483, 522, 783, 991
DpnII GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Eco130I CCWWGG 1 cut(s) 322
Eco32I GATATC 2 cut(s) 767, 1015
EcoRII CCWGG 4 cut(s) 8, 526, 713, 1008
EcoRV GATATC 2 cut(s) 767, 1015
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 6 cut(s) 189, 326, 376, 439, 538, 627
FatI CATG 6 cut(s) 185, 322, 372, 435, 534, 623
FauI CCCGC 2 cut(s) 587, 684
FauNDI CATATG 1 cut(s) 367
FblI GTMKAC 1 cut(s) 359
Fnu4HI GCNGC 3 cut(s) 582, 873, 897
FokI GGATG 5 cut(s) 49, 413, 415, 707, 899
Fsp4HI GCNGC 3 cut(s) 582, 873, 897
FspBI CTAG 2 cut(s) 479, 1041
GlaI GCGC 2 cut(s) 44, 314
GluI GCNGC 3 cut(s) 582, 873, 897
HaeII RGCGCY 1 cut(s) 316
HaeIII GGCC 1 cut(s) 504
HapII CCGG 3 cut(s) 25, 133, 734
HgaI GACGC 1 cut(s) 171
HhaI GCGC 2 cut(s) 45, 315
Hin1II CATG 6 cut(s) 189, 326, 376, 439, 538, 627
Hin6I GCGC 2 cut(s) 43, 313
HinP1I GCGC 2 cut(s) 43, 313
HinfI GANTC 4 cut(s) 59, 206, 218, 884
HpaII CCGG 3 cut(s) 25, 133, 734
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 3 cut(s) 360, 476, 665
Hpy188I TCNGA 2 cut(s) 115, 161
Hpy188III TCNNGA 4 cut(s) 152, 779, 847, 1018
Hpy8I GTNNAC 3 cut(s) 360, 476, 665
Hpy99I CGWCG 2 cut(s) 119, 165
HpyAV CCTTC 7 cut(s) 91, 442, 515, 644, 740, 833, 1015
HpyCH4III ACNGT 3 cut(s) 543, 631, 1000
HpyCH4V TGCA 9 cut(s) 546, 623, 627, 698, 803, 872, 896, 920, 995
HpyF10VI GCNNNNNNNGC 1 cut(s) 872
HpyF3I CTNAG 3 cut(s) 396, 614, 966
Hsp92II CATG 6 cut(s) 189, 326, 376, 439, 538, 627
HspAI GCGC 2 cut(s) 43, 313
Kzo9I GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
LmnI GCTCC 5 cut(s) 134, 324, 530, 643, 955
Lsp1109I GCAGC 3 cut(s) 593, 884, 908
LweI GCATC 3 cut(s) 183, 666, 685
MaeI CTAG 2 cut(s) 479, 1041
MalI GATC 7 cut(s) 150, 177, 243, 483, 522, 783, 991
MboI GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
MboII GAAGA 4 cut(s) 136, 461, 515, 914
MflI RGATCY 3 cut(s) 481, 781, 989
MhlI GDGCHC 1 cut(s) 778
MluCI AATT 5 cut(s) 489, 585, 618, 876, 1025
MmeI TCCRAC 4 cut(s) 138, 184, 296, 929
MseI TTAA 3 cut(s) 465, 557, 605
MslI CAYNNNNRTG 2 cut(s) 353, 798
MspI CCGG 3 cut(s) 25, 133, 734
MspR9I CCNGG 6 cut(s) 10, 133, 528, 715, 735, 1010
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 4 cut(s) 10, 528, 715, 1010
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 872
NciI CCSGG 2 cut(s) 133, 735
NcoI CCATGG 1 cut(s) 322
NdeI CATATG 1 cut(s) 367
NdeII GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
NlaIII CATG 6 cut(s) 189, 326, 376, 439, 538, 627
NlaIV GGNNCC 4 cut(s) 334, 532, 836, 910
NmeAIII GCCGAG 1 cut(s) 996
NruI TCGCGA 1 cut(s) 153
NspI RCATGY 1 cut(s) 627
PaeI GCATGC 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 1 cut(s) 958
PfeI GAWTC 4 cut(s) 59, 206, 218, 884
PfoI TCCNGGA 2 cut(s) 526, 1008
PkrI GCNGC 3 cut(s) 583, 874, 898
Ple19I CGATCG 1 cut(s) 151
Psp6I CCWGG 4 cut(s) 8, 526, 713, 1008
PspGI CCWGG 4 cut(s) 8, 526, 713, 1008
PspN4I GGNNCC 4 cut(s) 334, 532, 836, 910
PspPI GGNCC 1 cut(s) 502
PstI CTGCAG 1 cut(s) 874
PsuI RGATCY 3 cut(s) 481, 781, 989
PvuI CGATCG 1 cut(s) 151
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 2 cut(s) 100, 408
RsaNI GTAC 2 cut(s) 99, 407
RseI CAYNNNNRTG 2 cut(s) 353, 798
SaqAI TTAA 3 cut(s) 465, 557, 605
SatI GCNGC 3 cut(s) 582, 873, 897
Sau3AI GATC 7 cut(s) 148, 175, 241, 481, 520, 781, 989
Sau96I GGNCC 1 cut(s) 502
ScrFI CCNGG 6 cut(s) 10, 133, 528, 715, 735, 1010
SduI GDGCHC 1 cut(s) 778
SfaNI GCATC 3 cut(s) 183, 666, 685
SfcI CTRYAG 1 cut(s) 870
SmiMI CAYNNNNRTG 2 cut(s) 353, 798
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
SpeI ACTAGT 1 cut(s) 1040
SphI GCATGC 1 cut(s) 627
Sse9I AATT 5 cut(s) 489, 585, 618, 876, 1025
SsiI CCGC 3 cut(s) 90, 594, 691
SspMI CTAG 2 cut(s) 479, 1041
StyD4I CCNGG 6 cut(s) 8, 131, 526, 713, 733, 1008
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 3 cut(s) 543, 631, 1000
TaqI TCGA 4 cut(s) 147, 178, 244, 328
TasI AATT 5 cut(s) 489, 585, 618, 876, 1025
TfiI GAWTC 4 cut(s) 59, 206, 218, 884
Tru1I TTAA 3 cut(s) 465, 557, 605
Tru9I TTAA 3 cut(s) 465, 557, 605
TscAI CASTG 3 cut(s) 548, 634, 874
TseI GCWGC 3 cut(s) 581, 872, 896
TspDTI ATGAA 8 cut(s) 17, 174, 424, 429, 462, 497, 765, 951
TspGWI ACGGA 2 cut(s) 116, 132
TspRI CASTG 3 cut(s) 548, 634, 874
XceI RCATGY 1 cut(s) 627
XcmI CCANNNNNNNNNTGG 1 cut(s) 1025
XmiI GTMKAC 1 cut(s) 359
XspI CTAG 2 cut(s) 479, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.