MD08G1215200.v1.1

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013179
Physical Location & Seq
Reverse (-)
27817431 .. 27823225
5795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1215200.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGAGAGGCTTGTACCGGAGGAGCTCGCTATGGAGGACAAGAAGAGAAGCTCCGCACTCACTCTTCTTCTTCTCAAATGCTCTGTGTACCGACGCCAACATTGATGGGGTTCAAAGCTCCAGGATCAAGGTCTTCGATCGCCACTTCAGACGCAAACAGCTTGATCGAGCTGCTTGGTTGATGCGTTCCAAGGATTCTTTTGTTGATACTGTGGCTGAGAATCTATTGGATCGTTTGGAGGACTGCAAGAAAACATTTCCAGCGGCATTATGTTTAGGAGGTCAATTGGAAGCTGTCAGGCGCTTGTTACGTGGTCGTGGTGCCATTGAAAAGCTCATTATGATGTCTGCATCATCTGACATGGTAGAACTGTGTAGAAATGCTGAGCGTGAGTTACAAACTGAAAACATTGAAACATCCTTTATGGTTGGTGACGAAGAGTTTTTGCCCATTAAGGAAAATTCTGTGGATCTGGTATTAAGCTGCTTGGGACTTCACTGGACAAATGATCTTCCAGGAGCCATGATACAGTGCAGATTGGCATTGAAGCCAGATGGCCTATTTTTAGCAGCGATTCTGGGTGGAGAAACCTTAAAGTTAGAGCTGAGAATAGCGTGCACCGTGGCACAAATGGAGCGTGAAGGAGGCATCAGTCCACGAATATCACCTTTGGCGCAAGTGCGGGATGCAGGCAATCTTTTGACCAGGGCAGGCTTTACCCTTCCAGGTGTTGATGTGGACGAATTTGTAGTAAGATATCCAAGTGCTCTGGATCTCATAGAACATTTGCGTGCAATGGGTGAAACCAATGCTCTGCTACAAAGGAACACTATTTTAAAGAGAGAAATGGCCCTGGCCACCGCAGCAATTTATGATTCAATGTTTGCAGCAGAAGATGGCACCATCCCAGCAACCTTCCAGGTGATTTATATGACAGGGTGGAGGGACCATCCATCTCAGCAGAGGGCAAAGAGGAGGGGGTCGGCAACTGTATCTTTTCAGGACATCCAGAAGCAATTTGGAAGTGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

345

Amino Acids

38.65

Weight (kDa)

6.97

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 100 - 189 7.6e-11 Methyltransferase domain
Methyltransf_25 PF13649 109 - 186 2e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22800
fragaria_vesca FvH4_5g33060
malus_domestica MD04G1240000.v1.1 MD08G1215200.v1.1 MD15G1399100.v1.1
prunus_persica Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1 Prupe.1G530500_v2.0.a1
pyrus_communis pycom08g18630 pycom15g35810
rosa_chinensis RchiOBHm_Chr7g0231231
rosa_laevigata RLG00000001427
rosa_multiflora Rmu_sc0004571.1_g000020
rosa_roxburghii Rroxscaffold_3G00229420
rosa_rugosa Rorug07G0265200
rosa_samantha Rh7BG394200 Rh7CG438300
rosa_wichuraiana Rw7G034630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 320, 899
AciI CCGC 4 cut(s) 53, 263, 682, 861
AclWI GGATC 4 cut(s) 131, 237, 477, 780
AcoI YGGCCR 1 cut(s) 855
AcsI RAATTY 2 cut(s) 460, 743
AcuI CTGAAG 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 14, 88
AgsI TTSAA 5 cut(s) 113, 329, 413, 547, 879
AjnI CCWGG 6 cut(s) 119, 514, 704, 724, 852, 918
AluBI AGCT 9 cut(s) 24, 50, 117, 160, 170, 293, 334, 483, 604
AluI AGCT 9 cut(s) 24, 50, 117, 160, 170, 293, 334, 483, 604
Alw21I GWGCWC 3 cut(s) 26, 620, 769
Alw44I GTGCAC 1 cut(s) 616
AlwI GGATC 4 cut(s) 131, 237, 477, 780
AoxI GGCC 3 cut(s) 556, 849, 855
ApaLI GTGCAC 1 cut(s) 616
ApeKI GCWGC 5 cut(s) 170, 483, 569, 863, 887
ApoI RAATTY 2 cut(s) 460, 743
AspLEI GCGC 2 cut(s) 303, 676
AspS9I GGNCC 2 cut(s) 850, 946
AsuHPI GGTGA 4 cut(s) 443, 657, 812, 934
AvaII GGWCC 1 cut(s) 946
BaeGI GKGCMC 1 cut(s) 620
BalI TGGCCA 1 cut(s) 857
BanI GGYRCC 2 cut(s) 320, 899
BanII GRGCYC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 3 cut(s) 26, 620, 769
BbvI GCAGC 5 cut(s) 157, 470, 581, 875, 899
BccI CCATC 6 cut(s) 98, 548, 890, 911, 957, 961
BciT130I CCWGG 6 cut(s) 121, 516, 706, 726, 854, 920
BfoI RGCGCY 1 cut(s) 304
BisI GCNGC 6 cut(s) 171, 264, 484, 570, 864, 888
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 6 cut(s) 172, 265, 485, 571, 865, 889
Bme1390I CCNGG 6 cut(s) 121, 516, 706, 726, 854, 920
Bme18I GGWCC 1 cut(s) 946
BmgT120I GGNCC 2 cut(s) 850, 946
BmiI GGNNCC 4 cut(s) 322, 520, 901, 947
BmrFI CCNGG 6 cut(s) 121, 516, 706, 726, 854, 920
BmsI GCATC 4 cut(s) 171, 359, 657, 676
BpiI GAAGAC 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 103
Bpu1102I GCTNAGC 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 4 cut(s) 189, 621, 705, 852
BsaWI WCCGGW 1 cut(s) 15
Bse1I ACTGG 1 cut(s) 503
Bse3DI GCAATG 1 cut(s) 801
BseBI CCWGG 6 cut(s) 121, 516, 706, 726, 854, 920
BseDI CCNNGG 4 cut(s) 189, 621, 705, 852
BseGI GGATG 5 cut(s) 416, 691, 903, 949, 1005
BseMI GCAATG 1 cut(s) 801
BseMII CTCAG 4 cut(s) 207, 375, 596, 971
BseNI ACTGG 1 cut(s) 503
BseRI GAGGAG 2 cut(s) 34, 988
BseSI GKGCMC 1 cut(s) 620
BseXI GCAGC 5 cut(s) 157, 470, 581, 875, 899
BseYI CCCAGC 1 cut(s) 907
BsgI GTGCAG 1 cut(s) 553
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 3 cut(s) 558, 851, 857
BshNI GGYRCC 2 cut(s) 320, 899
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 3 cut(s) 26, 620, 769
BsiSI CCGG 1 cut(s) 16
BslFI GGGAC 2 cut(s) 504, 959
BsmFI GGGAC 2 cut(s) 504, 959
BsnI GGCC 3 cut(s) 558, 851, 857
Bsp1286I GDGCHC 3 cut(s) 26, 620, 769
Bsp143I GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 4 cut(s) 53, 263, 682, 861
BspANI GGCC 3 cut(s) 558, 851, 857
BspCNI CTCAG 4 cut(s) 208, 376, 597, 970
BspLI GGNNCC 4 cut(s) 322, 520, 901, 947
BspPI GGATC 4 cut(s) 131, 237, 477, 780
BspT107I GGYRCC 2 cut(s) 320, 899
BsrDI GCAATG 1 cut(s) 801
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 4 cut(s) 189, 621, 705, 852
BssMI GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 6 cut(s) 121, 516, 706, 726, 854, 920
Bst4CI ACNGT 5 cut(s) 211, 372, 531, 622, 991
Bst6I CTCTTC 3 cut(s) 37, 68, 432
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 5 cut(s) 26, 616, 691, 712, 792
BstDEI CTNAG 4 cut(s) 216, 384, 605, 957
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 5 cut(s) 416, 691, 903, 949, 1005
BstH2I RGCGCY 1 cut(s) 304
BstHHI GCGC 2 cut(s) 303, 676
BstKTI GATC 7 cut(s) 126, 139, 166, 232, 472, 511, 775
BstMBI GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 863
BstNI CCWGG 6 cut(s) 121, 516, 706, 726, 854, 920
BstSCI CCNGG 6 cut(s) 119, 514, 704, 724, 852, 918
BstSLI GKGCMC 1 cut(s) 620
BstV1I GCAGC 5 cut(s) 157, 470, 581, 875, 899
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 2 cut(s) 469, 772
BstYI RGATCY 2 cut(s) 469, 772
BsuRI GGCC 3 cut(s) 558, 851, 857
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 5 cut(s) 416, 691, 903, 949, 1005
BtsIMutI CAGTG 2 cut(s) 496, 536
Cac8I GCNNGC 5 cut(s) 26, 616, 691, 712, 792
CfoI GCGC 2 cut(s) 303, 676
Cfr13I GGNCC 2 cut(s) 850, 946
CseI GACGC 2 cut(s) 101, 159
Csp6I GTAC 2 cut(s) 13, 87
CviAII CATG 2 cut(s) 361, 523
CviQI GTAC 2 cut(s) 13, 87
DdeI CTNAG 4 cut(s) 216, 384, 605, 957
DpnI GATC 7 cut(s) 125, 138, 165, 231, 471, 510, 774
DpnII GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
DraI TTTAAA 1 cut(s) 837
EaeI YGGCCR 1 cut(s) 855
Eam1104I CTCTTC 3 cut(s) 37, 68, 432
EarI CTCTTC 3 cut(s) 37, 68, 432
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 26
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 946
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 24
EcoRII CCWGG 6 cut(s) 119, 514, 704, 724, 852, 918
EcoRV GATATC 1 cut(s) 758
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 2 cut(s) 364, 526
FaqI GGGAC 2 cut(s) 504, 959
FatI CATG 2 cut(s) 360, 522
FauI CCCGC 1 cut(s) 675
Fnu4HI GCNGC 6 cut(s) 171, 264, 484, 570, 864, 888
FokI GGATG 5 cut(s) 403, 698, 890, 936, 992
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 6 cut(s) 171, 264, 484, 570, 864, 888
GlaI GCGC 2 cut(s) 302, 675
GluI GCNGC 6 cut(s) 171, 264, 484, 570, 864, 888
GsaI CCCAGC 1 cut(s) 911
GsuI CTGGAG 1 cut(s) 103
HaeII RGCGCY 1 cut(s) 304
HaeIII GGCC 3 cut(s) 558, 851, 857
HapII CCGG 1 cut(s) 16
HgaI GACGC 2 cut(s) 101, 159
HhaI GCGC 2 cut(s) 303, 676
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 2 cut(s) 364, 526
Hin6I GCGC 2 cut(s) 301, 674
HinP1I GCGC 2 cut(s) 301, 674
HinfI GANTC 4 cut(s) 194, 220, 574, 875
HpaII CCGG 1 cut(s) 16
HphI GGTGA 4 cut(s) 443, 657, 812, 934
Hpy166II GTNNAC 4 cut(s) 87, 618, 656, 739
Hpy188I TCNGA 2 cut(s) 149, 358
Hpy188III TCNNGA 3 cut(s) 770, 1001, 1009
Hpy8I GTNNAC 4 cut(s) 87, 618, 656, 739
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 3 cut(s) 635, 731, 925
HpyCH4III ACNGT 5 cut(s) 211, 372, 531, 622, 991
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 7 cut(s) 246, 350, 534, 618, 689, 794, 887
HpyF10VI GCNNNNNNNGC 1 cut(s) 863
HpyF3I CTNAG 4 cut(s) 216, 384, 605, 957
HpySE526I ACGT 1 cut(s) 310
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 2 cut(s) 364, 526
HspAI GCGC 2 cut(s) 301, 674
Kzo9I GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
LmnI GCTCC 5 cut(s) 21, 55, 122, 518, 634
Lsp1109I GCAGC 5 cut(s) 157, 470, 581, 875, 899
LweI GCATC 4 cut(s) 171, 359, 657, 676
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 3 cut(s) 306, 393, 431
MalI GATC 7 cut(s) 125, 138, 165, 231, 471, 510, 774
MboI GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
MboII GAAGA 8 cut(s) 54, 55, 58, 61, 124, 449, 503, 905
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 2 cut(s) 469, 772
MhlI GDGCHC 3 cut(s) 26, 620, 769
MlsI TGGCCA 1 cut(s) 857
MluCI AATT 5 cut(s) 284, 460, 743, 867, 1016
MluNI TGGCCA 1 cut(s) 857
Mox20I TGGCCA 1 cut(s) 857
MscI TGGCCA 1 cut(s) 857
MseI TTAA 4 cut(s) 453, 479, 593, 836
MslI CAYNNNNRTG 2 cut(s) 341, 789
Msp20I TGGCCA 1 cut(s) 857
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 6 cut(s) 121, 516, 706, 726, 854, 920
MunI CAATTG 1 cut(s) 284
MvaI CCWGG 6 cut(s) 121, 516, 706, 726, 854, 920
MwoI GCNNNNNNNGC 1 cut(s) 863
NdeII GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
NlaIII CATG 2 cut(s) 364, 526
NlaIV GGNNCC 4 cut(s) 322, 520, 901, 947
NmuCI GTSAC 1 cut(s) 431
PfeI GAWTC 4 cut(s) 194, 220, 574, 875
PfoI TCCNGGA 2 cut(s) 119, 514
PkrI GCNGC 6 cut(s) 172, 265, 485, 571, 865, 889
Ple19I CGATCG 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 26
Psp6I CCWGG 6 cut(s) 119, 514, 704, 724, 852, 918
PspFI CCCAGC 1 cut(s) 907
PspGI CCWGG 6 cut(s) 119, 514, 704, 724, 852, 918
PspN4I GGNNCC 4 cut(s) 322, 520, 901, 947
PspPI GGNCC 2 cut(s) 850, 946
PsuI RGATCY 2 cut(s) 469, 772
PvuI CGATCG 1 cut(s) 139
RsaI GTAC 2 cut(s) 14, 88
RsaNI GTAC 2 cut(s) 13, 87
RseI CAYNNNNRTG 2 cut(s) 341, 789
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 4 cut(s) 453, 479, 593, 836
SatI GCNGC 6 cut(s) 171, 264, 484, 570, 864, 888
Sau3AI GATC 7 cut(s) 123, 136, 163, 229, 469, 508, 772
Sau96I GGNCC 2 cut(s) 850, 946
ScrFI CCNGG 6 cut(s) 121, 516, 706, 726, 854, 920
SduI GDGCHC 3 cut(s) 26, 620, 769
SfaNI GCATC 4 cut(s) 171, 359, 657, 676
SinI GGWCC 1 cut(s) 946
SmiMI CAYNNNNRTG 2 cut(s) 341, 789
Sse9I AATT 5 cut(s) 284, 460, 743, 867, 1016
SsiI CCGC 4 cut(s) 53, 263, 682, 861
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 6 cut(s) 119, 514, 704, 724, 852, 918
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 5 cut(s) 211, 372, 531, 622, 991
TaiI ACGT 1 cut(s) 313
TaqI TCGA 2 cut(s) 135, 166
TasI AATT 5 cut(s) 284, 460, 743, 867, 1016
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 4 cut(s) 194, 220, 574, 875
Tru1I TTAA 4 cut(s) 453, 479, 593, 836
Tru9I TTAA 4 cut(s) 453, 479, 593, 836
TscAI CASTG 2 cut(s) 503, 536
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 5 cut(s) 170, 483, 569, 863, 887
Tsp45I GTSAC 1 cut(s) 431
TspRI CASTG 2 cut(s) 503, 536
VneI GTGCAC 1 cut(s) 616
VpaK11BI GGWCC 1 cut(s) 946
XapI RAATTY 2 cut(s) 460, 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.