FvH4_5g34310

U4 U6.U5 tri-snRNP-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012539
Physical Location & Seq
Forward (+)
24991391 .. 24995292
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34310.t1

Sequence Viewer

Length: 1161 bp
ATGGAAAGCTCACTCAAAAACCAAGCAAGTGAGAGAACAAGGAGGCTCAAAGATATAATTCTTAAAAAGAAAGAAGAGAGCGTGACCGGTGTAAAGTCAATTGCACTCTATGCCGCCACTGCAATCCCCTCCAGTGAGTCGTCTTCCCCCAAGGTTGGCTTAACATACATGGACAACTTCGTCTGCGAACTCCAGCTTAATTATGATGAATATTCCTACAACCATAAGCCTCAGTTTCTTGAGCAAGTTGCTGATTCTGATAAACTAAAACAAAAACAAGATGCTGATGAATATTTACAATTAAAAGAAGATGAAAATCAAGTAGTAATCCTGGATGACAAGGAAGCAAGTCCGGCTGATGTAACTATATGTGAAGCAGCGATTGGAAAAGGGAAGTGGATATTCAAATTGAAAGAACCGATGAGTTTGGCCGAAGATTTGACAAAGAAAACAGCTTATAAGAGATTTTGTCATACCTTTCATGGGAAAGGGTCTGGAGCAAGGAAGCAAGAAAAGCGCAGAAAAGCAACACCTAAGGCAGTGGAAAGAGTAGTAGAAGCAAATTCGAGAGGGCGCGATCCTTTCGATAGAAAAGATTATCTAGCGGCTTTTGATGCATATACTCAGCGACCGCCACTCATGGAGGAGCATTGTGGTTTCGCGACCTATGGTCCAGAGAGATCATCAAGGGCAGCAACGGAGGAGATCTTTCCGTCAACCCTAGCACAGTCCCTCTTCAGTGGGGAGGCAGGATTCTTGGGTGTTGATCGGGATCGAAGAACGGTTTTGGGTGCGTGCTTTGATTGGTGTGGGTCAAGTTTGAGCGGCGACGGCAGTGTAGTTTGGTGGAGAGGGCTTGCTTTAGAGGCGACCTCTATTGTTGTTGACGGGAGGATGAAGGATTTGTCTCATCTCTCCTCCTTGAATTGCAGTGGTGTGGTGATGGTGTCAATGGTGAGGTGGCATCGGGGACGTCCGCCGGAGGAGATGGCGGCAGCAGCAGCATGCTTGCAACTTGGGTGCTCATCTTGCTGGGTGCCCTTAGCAGTTGGGCCTTGGCTCTTTTGGGCTTGTGTTCATAATGTTGAGGCTTTTCTTCCTAGGGTTGGGCCTAGTCTGTCATGGTCTTTTCGGGCCAAACGAACTAGGTTGAATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SART-1

Protein Analysis

387

Amino Acids

43.07

Weight (kDa)

8.39

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SART-1 PF03343 146 - 175 3.4e-07 SART-1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AasI GACNNNNNNGTC 1 cut(s) 179
AatII GACGTC 1 cut(s) 976
AccB1I GGYRCC 1 cut(s) 1036
AccBSI CCGCTC 1 cut(s) 825
AccII CGCG 2 cut(s) 576, 662
AciI CCGC 6 cut(s) 114, 605, 632, 825, 977, 992
AclWI GGATC 2 cut(s) 572, 780
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 1 cut(s) 562
AcuI CTGAAG 1 cut(s) 721
AcyI GRCGYC 1 cut(s) 973
AfiI CCNNNNNNNGG 3 cut(s) 155, 483, 1106
AgeI ACCGGT 1 cut(s) 86
AgsI TTSAA 4 cut(s) 406, 412, 925, 1153
AhdI GACNNNNNGTC 1 cut(s) 669
AjnI CCWGG 1 cut(s) 330
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AluBI AGCT 3 cut(s) 9, 196, 455
AluI AGCT 3 cut(s) 9, 196, 455
Alw21I GWGCWC 1 cut(s) 1025
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 2 cut(s) 572, 780
AoxI GGCC 4 cut(s) 429, 1052, 1109, 1134
ApeKI GCWGC 5 cut(s) 377, 692, 995, 998, 1001
ApoI RAATTY 1 cut(s) 562
AsiGI ACCGGT 1 cut(s) 86
AspA2I CCTAGG 1 cut(s) 1100
AspLEI GCGC 2 cut(s) 519, 576
AspS9I GGNCC 4 cut(s) 671, 1052, 1109, 1134
AsuHPI GGTGA 2 cut(s) 952, 967
AvaII GGWCC 1 cut(s) 671
AvrII CCTAGG 1 cut(s) 1100
AxyI CCTNAGG 1 cut(s) 534
BaeGI GKGCMC 1 cut(s) 1041
BanI GGYRCC 1 cut(s) 1036
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 1025
BbvI GCAGC 5 cut(s) 389, 704, 1007, 1010, 1013
BccI CCATC 2 cut(s) 937, 982
BceAI ACGGC 1 cut(s) 847
BciT130I CCWGG 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 5 cut(s) 602, 722, 1101, 1113, 1146
BglII AGATCT 1 cut(s) 705
BisI GCNGC 9 cut(s) 114, 378, 606, 693, 826, 993, 996, 999, 1002
BlnI CCTAGG 1 cut(s) 1100
BlsI GCNGC 9 cut(s) 115, 379, 607, 694, 827, 994, 997, 1000, 1003
Bme1390I CCNGG 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 671
BmeRI GACNNNNNGTC 1 cut(s) 669
BmgT120I GGNCC 4 cut(s) 671, 1052, 1109, 1134
BmiI GGNNCC 1 cut(s) 1038
BmrFI CCNGG 1 cut(s) 332
BmsI GCATC 3 cut(s) 271, 604, 973
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 857, 889
BpmI CTGGAG 3 cut(s) 115, 176, 516
Bpu10I CCTNAGC 1 cut(s) 1042
BpuEI CTTGAG 1 cut(s) 260
BsaBI GATNNNNATC 2 cut(s) 315, 771
BsaHI GRCGYC 1 cut(s) 973
BsaJI CCNNGG 3 cut(s) 150, 1055, 1100
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 483, 1106
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 132
Bse21I CCTNAGG 1 cut(s) 534
Bse8I GATNNNNATC 2 cut(s) 315, 771
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 3 cut(s) 150, 1055, 1100
BseGI GGATG 2 cut(s) 340, 900
BseJI GATNNNNATC 2 cut(s) 315, 771
BseLI CCNNNNNNNGG 3 cut(s) 155, 483, 1106
BseMII CTCAG 2 cut(s) 245, 638
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 4 cut(s) 659, 716, 907, 998
BseSI GKGCMC 1 cut(s) 1041
BseXI GCAGC 5 cut(s) 389, 704, 1007, 1010, 1013
BseYI CCCAGC 1 cut(s) 1032
Bsh1236I CGCG 2 cut(s) 576, 662
Bsh1285I CGRYCG 1 cut(s) 632
BshFI GGCC 4 cut(s) 431, 1054, 1111, 1136
BshNI GGYRCC 1 cut(s) 1036
BshTI ACCGGT 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 632
BsiHKAI GWGCWC 1 cut(s) 1025
BsiSI CCGG 3 cut(s) 87, 353, 980
BslFI GGGAC 2 cut(s) 715, 984
BslI CCNNNNNNNGG 3 cut(s) 155, 483, 1106
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 2 cut(s) 715, 984
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 4 cut(s) 431, 1054, 1111, 1136
Bsp1286I GDGCHC 2 cut(s) 1025, 1041
Bsp143I GATC 5 cut(s) 577, 680, 705, 766, 772
Bsp68I TCGCGA 1 cut(s) 662
BspACI CCGC 6 cut(s) 114, 605, 632, 825, 977, 992
BspANI GGCC 4 cut(s) 431, 1054, 1111, 1136
BspCNI CTCAG 2 cut(s) 244, 637
BspFNI CGCG 2 cut(s) 576, 662
BspLI GGNNCC 1 cut(s) 1038
BspPI GGATC 2 cut(s) 572, 780
BspT107I GGYRCC 1 cut(s) 1036
BsrBI CCGCTC 1 cut(s) 825
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 3 cut(s) 150, 1055, 1100
BssMI GATC 5 cut(s) 577, 680, 705, 766, 772
BssNI GRCGYC 1 cut(s) 973
BssT1I CCWWGG 3 cut(s) 150, 1055, 1100
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 2 cut(s) 729, 784
Bst6I CTCTTC 2 cut(s) 69, 740
BstACI GRCGYC 1 cut(s) 973
BstAPI GCANNNNNTGC 1 cut(s) 110
BstC8I GCNNGC 4 cut(s) 796, 858, 1006, 1010
BstDEI CTNAG 4 cut(s) 231, 534, 624, 1042
BstF5I GGATG 2 cut(s) 340, 900
BstFNI CGCG 2 cut(s) 576, 662
BstHHI GCGC 2 cut(s) 519, 576
BstKTI GATC 5 cut(s) 580, 683, 708, 769, 775
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 5 cut(s) 577, 680, 705, 766, 772
BstMCI CGRYCG 1 cut(s) 632
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 1008
BstSCI CCNGG 1 cut(s) 330
BstSLI GKGCMC 1 cut(s) 1041
BstUI CGCG 2 cut(s) 576, 662
BstV1I GCAGC 5 cut(s) 389, 704, 1007, 1010, 1013
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 705
BstYI RGATCY 1 cut(s) 705
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 4 cut(s) 431, 1054, 1111, 1136
BtsCI GGATG 2 cut(s) 340, 900
BtsI GCAGTG 4 cut(s) 117, 546, 841, 937
BtsIMutI CAGTG 6 cut(s) 117, 139, 546, 745, 841, 937
BtuMI TCGCGA 1 cut(s) 662
Cac8I GCNNGC 4 cut(s) 796, 858, 1006, 1010
CfoI GCGC 2 cut(s) 519, 576
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 4 cut(s) 671, 1052, 1109, 1134
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 5 cut(s) 169, 482, 640, 1005, 1122
DdeI CTNAG 4 cut(s) 231, 534, 624, 1042
DpnI GATC 5 cut(s) 579, 682, 707, 768, 774
DpnII GATC 5 cut(s) 577, 680, 705, 766, 772
DrdI GACNNNNNNGTC 1 cut(s) 179
DriI GACNNNNNGTC 1 cut(s) 669
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 2 cut(s) 69, 740
Eam1105I GACNNNNNGTC 1 cut(s) 669
EarI CTCTTC 2 cut(s) 69, 740
EciI GGCGGA 1 cut(s) 966
Eco130I CCWWGG 3 cut(s) 150, 1055, 1100
Eco47I GGWCC 1 cut(s) 671
Eco57I CTGAAG 1 cut(s) 721
Eco81I CCTNAGG 1 cut(s) 534
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 3 cut(s) 150, 1055, 1100
EcoT22I ATGCAT 1 cut(s) 619
ErhI CCWWGG 3 cut(s) 150, 1055, 1100
FaeI CATG 5 cut(s) 172, 485, 643, 1008, 1125
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 715, 984
FatI CATG 5 cut(s) 168, 481, 639, 1004, 1121
Fnu4HI GCNGC 9 cut(s) 114, 378, 606, 693, 826, 993, 996, 999, 1002
FokI GGATG 2 cut(s) 347, 907
Fsp4HI GCNGC 9 cut(s) 114, 378, 606, 693, 826, 993, 996, 999, 1002
FspBI CTAG 5 cut(s) 602, 722, 1101, 1113, 1146
GlaI GCGC 2 cut(s) 518, 575
GluI GCNGC 9 cut(s) 114, 378, 606, 693, 826, 993, 996, 999, 1002
GsaI CCCAGC 1 cut(s) 1036
GsuI CTGGAG 3 cut(s) 115, 176, 516
HaeIII GGCC 4 cut(s) 431, 1054, 1111, 1136
HapII CCGG 3 cut(s) 87, 353, 980
HhaI GCGC 2 cut(s) 519, 576
Hin1I GRCGYC 1 cut(s) 973
Hin1II CATG 5 cut(s) 172, 485, 643, 1008, 1125
Hin6I GCGC 2 cut(s) 517, 574
HinP1I GCGC 2 cut(s) 517, 574
HincII GTYRAC 2 cut(s) 717, 886
HindII GTYRAC 2 cut(s) 717, 886
HinfI GANTC 3 cut(s) 137, 254, 753
HpaII CCGG 3 cut(s) 87, 353, 980
HphI GGTGA 2 cut(s) 952, 967
Hpy166II GTNNAC 2 cut(s) 717, 886
Hpy188I TCNGA 1 cut(s) 259
Hpy188III TCNNGA 6 cut(s) 239, 495, 567, 661, 674, 770
Hpy8I GTNNAC 2 cut(s) 717, 886
Hpy99I CGWCG 1 cut(s) 833
HpyAV CCTTC 1 cut(s) 892
HpyCH4III ACNGT 2 cut(s) 729, 784
HpyCH4IV ACGT 1 cut(s) 973
HpyCH4V TGCA 5 cut(s) 104, 122, 617, 930, 1012
HpyF3I CTNAG 4 cut(s) 231, 534, 624, 1042
HpySE526I ACGT 1 cut(s) 973
Hsp92I GRCGYC 1 cut(s) 973
Hsp92II CATG 5 cut(s) 172, 485, 643, 1008, 1125
HspAI GCGC 2 cut(s) 517, 574
Kzo9I GATC 5 cut(s) 577, 680, 705, 766, 772
LmnI GCTCC 2 cut(s) 497, 646
Lsp1109I GCAGC 5 cut(s) 389, 704, 1007, 1010, 1013
LweI GCATC 3 cut(s) 271, 604, 973
MaeI CTAG 5 cut(s) 602, 722, 1101, 1113, 1146
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 2 cut(s) 82, 361
MalI GATC 5 cut(s) 579, 682, 707, 768, 774
MbiI CCGCTC 1 cut(s) 825
MboI GATC 5 cut(s) 577, 680, 705, 766, 772
MboII GAAGA 7 cut(s) 86, 135, 320, 446, 727, 789, 1088
MfeI CAATTG 1 cut(s) 99
MflI RGATCY 1 cut(s) 705
MhlI GDGCHC 2 cut(s) 1025, 1041
MluCI AATT 7 cut(s) 57, 99, 199, 299, 407, 562, 925
MlyI GAGTC 1 cut(s) 146
Mph1103I ATGCAT 1 cut(s) 619
MseI TTAA 4 cut(s) 63, 161, 198, 302
MspI CCGG 3 cut(s) 87, 353, 980
MspR9I CCNGG 1 cut(s) 332
MunI CAATTG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 1159
MvaI CCWGG 1 cut(s) 332
MvnI CGCG 2 cut(s) 576, 662
NdeII GATC 5 cut(s) 577, 680, 705, 766, 772
NlaIII CATG 5 cut(s) 172, 485, 643, 1008, 1125
NlaIV GGNNCC 1 cut(s) 1038
NmuCI GTSAC 1 cut(s) 82
NruI TCGCGA 1 cut(s) 662
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 1 cut(s) 1008
PaeI GCATGC 1 cut(s) 1008
PcsI WCGNNNNNNNCGW 1 cut(s) 1138
PctI GAATGC 1 cut(s) 1159
PfeI GAWTC 2 cut(s) 254, 753
PfoI TCCNGGA 1 cut(s) 330
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 9 cut(s) 115, 379, 607, 694, 827, 994, 997, 1000, 1003
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 1032
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 1038
PspPI GGNCC 4 cut(s) 671, 1052, 1109, 1134
PsuI RGATCY 1 cut(s) 705
RruI TCGCGA 1 cut(s) 662
SaqAI TTAA 4 cut(s) 63, 161, 198, 302
SatI GCNGC 9 cut(s) 114, 378, 606, 693, 826, 993, 996, 999, 1002
Sau3AI GATC 5 cut(s) 577, 680, 705, 766, 772
Sau96I GGNCC 4 cut(s) 671, 1052, 1109, 1134
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 332
SduI GDGCHC 2 cut(s) 1025, 1041
SfaNI GCATC 3 cut(s) 271, 604, 973
SinI GGWCC 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
SphI GCATGC 1 cut(s) 1008
Sse9I AATT 7 cut(s) 57, 99, 199, 299, 407, 562, 925
SsiI CCGC 6 cut(s) 114, 605, 632, 825, 977, 992
SspI AATATT 2 cut(s) 212, 293
SspMI CTAG 5 cut(s) 602, 722, 1101, 1113, 1146
StyD4I CCNGG 1 cut(s) 330
StyI CCWWGG 3 cut(s) 150, 1055, 1100
TaaI ACNGT 2 cut(s) 729, 784
TaiI ACGT 1 cut(s) 976
TaqI TCGA 3 cut(s) 566, 585, 775
TasI AATT 7 cut(s) 57, 99, 199, 299, 407, 562, 925
TauI GCSGC 4 cut(s) 116, 608, 828, 995
TfiI GAWTC 2 cut(s) 254, 753
Tru1I TTAA 4 cut(s) 63, 161, 198, 302
Tru9I TTAA 4 cut(s) 63, 161, 198, 302
TscAI CASTG 6 cut(s) 124, 139, 546, 745, 841, 937
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 5 cut(s) 377, 692, 995, 998, 1001
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 6 cut(s) 222, 303, 327, 470, 911, 1067
TspGWI ACGGA 2 cut(s) 702, 713
TspRI CASTG 6 cut(s) 124, 139, 546, 745, 841, 937
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 1 cut(s) 562
XceI RCATGY 1 cut(s) 1008
XmaJI CCTAGG 1 cut(s) 1100
XspI CTAG 5 cut(s) 602, 722, 1101, 1113, 1146
ZraI GACGTC 1 cut(s) 974
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.