MD07G1289000.v1.1

U4 U6.U5 tri-snRNP-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012539
Physical Location & Seq
Reverse (-)
34988768 .. 34989449
682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1289000.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGAATTGATCGCGAAGACAAAGAACACATTAATCCTCCTGCAGAGGACCATAAGGAGGAGAAGGGCGTTTCAGATGGAATTTTTCGCGGTTGGGAAAGGCTTATCGGGTGCTCTGAAGCTGCTGAAAGAGCGAGGAAGCCTTGAAGTAAAACATGATACGGATGCAGTTCGGATGACTGATGAATTAGGTCGGCCTATGACGCAAAAGGAAGCGTATAACAGACTTTCGCACGCATTCCACAGGAAGGGGCCTGGCAAAAGGAAGCAAGAAAAGTGCAGGAAGCAATTCGAGAAGGAACAGAAACTGAAGCAGATGAGTAGTTCAGACACGCCATTACAGTCCATGGAGACAGTGAGGGAAGCTCAAGCTCAGTTGCGAAAACCCTACCTTGTCCTCAGCGGTCACGTTCATCCGGGTCAGACAAGCGATCCAGCGAGCGGATTTGCCACTGTCGAACAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SART-1

Protein Analysis

156

Amino Acids

17.74

Weight (kDa)

10.18

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SART-1 PF03343 59 - 100 2.5e-13 SART-1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 441
AccII CGCG 2 cut(s) 14, 89
AciI CCGC 3 cut(s) 89, 402, 441
AclWI GGATC 1 cut(s) 425
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 2 cut(s) 137, 329
AfiI CCNNNNNNNGG 3 cut(s) 57, 247, 440
AgsI TTSAA 1 cut(s) 146
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 3 cut(s) 121, 365, 371
AluI AGCT 3 cut(s) 121, 365, 371
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 1 cut(s) 425
AlwNI CAGNNNCTG 1 cut(s) 307
AoxI GGCC 2 cut(s) 194, 251
ApeKI GCWGC 1 cut(s) 121
ApoI RAATTY 1 cut(s) 80
AseI ATTAAT 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 48, 251
AsuC2I CCSGG 1 cut(s) 417
AvaII GGWCC 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 115
BbvCI CCTCAGC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 255
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 344
BfmI CTRYAG 1 cut(s) 41
BisI GCNGC 1 cut(s) 122
BlsI GCNGC 1 cut(s) 123
Bme1390I CCNGG 2 cut(s) 255, 417
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 2 cut(s) 48, 251
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 2 cut(s) 255, 417
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 23
BplI GAGNNNNNCTC 2 cut(s) 349, 381
Bpu10I CCTNAGC 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 351
BpuMI CCSGG 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 345
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 247, 440
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 3 cut(s) 169, 180, 412
BseLI CCNNNNNNNGG 3 cut(s) 57, 247, 440
BseMII CTCAG 2 cut(s) 386, 412
BseRI GAGGAG 1 cut(s) 73
BseXI GCAGC 1 cut(s) 108
BsgI GTGCAG 1 cut(s) 298
Bsh1236I CGCG 2 cut(s) 14, 89
BshFI GGCC 2 cut(s) 196, 253
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 416
BslI CCNNNNNNNGG 3 cut(s) 57, 247, 440
BsmAI GTCTC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 236
BsnI GGCC 2 cut(s) 196, 253
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 9, 430
Bsp19I CCATGG 1 cut(s) 345
Bsp68I TCGCGA 1 cut(s) 14
BspACI CCGC 3 cut(s) 89, 402, 441
BspANI GGCC 2 cut(s) 196, 253
BspCNI CTCAG 2 cut(s) 385, 411
BspFNI CGCG 2 cut(s) 14, 89
BspLI GGNNCC 1 cut(s) 252
BspMAI CTGCAG 1 cut(s) 45
BspPI GGATC 1 cut(s) 425
BsrBI CCGCTC 1 cut(s) 441
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 2 cut(s) 9, 430
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 4 cut(s) 342, 355, 454, 462
BstC8I GCNNGC 2 cut(s) 234, 439
BstDEI CTNAG 2 cut(s) 372, 398
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 3 cut(s) 169, 180, 412
BstFNI CGCG 2 cut(s) 14, 89
BstKTI GATC 2 cut(s) 12, 433
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 2 cut(s) 9, 430
BstMWI GCNNNNNNNGC 2 cut(s) 130, 202
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 2 cut(s) 253, 415
BstSFI CTRYAG 1 cut(s) 41
BstUI CGCG 2 cut(s) 14, 89
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 196, 253
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 3 cut(s) 169, 180, 412
BtsIMutI CAGTG 2 cut(s) 360, 450
BtuMI TCGCGA 1 cut(s) 14
Cac8I GCNNGC 2 cut(s) 234, 439
CaiI CAGNNNCTG 1 cut(s) 307
Cfr13I GGNCC 2 cut(s) 48, 251
CseI GACGC 1 cut(s) 211
CviAII CATG 2 cut(s) 155, 346
CviJI RGCY 7 cut(s) 102, 121, 141, 196, 253, 365, 371
CviKI_1 RGCY 7 cut(s) 102, 121, 141, 196, 253, 365, 371
DdeI CTNAG 2 cut(s) 372, 398
DpnI GATC 2 cut(s) 11, 432
DpnII GATC 2 cut(s) 9, 430
Eco130I CCWWGG 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 48
Eco57I CTGAAG 2 cut(s) 137, 329
EcoO109I RGGNCCY 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 2 cut(s) 158, 349
FaiI YATR 5 cut(s) 53, 156, 200, 219, 347
FatI CATG 2 cut(s) 154, 345
Fnu4HI GCNGC 1 cut(s) 122
FokI GGATG 3 cut(s) 176, 187, 399
Fsp4HI GCNGC 1 cut(s) 122
GluI GCNGC 1 cut(s) 122
HaeIII GGCC 2 cut(s) 196, 253
HapII CCGG 1 cut(s) 416
HgaI GACGC 1 cut(s) 211
Hin1II CATG 2 cut(s) 158, 349
HpaII CCGG 1 cut(s) 416
Hpy188I TCNGA 6 cut(s) 75, 117, 174, 328, 423, 467
Hpy188III TCNNGA 2 cut(s) 13, 292
HpyAV CCTTC 3 cut(s) 57, 241, 289
HpyCH4III ACNGT 4 cut(s) 342, 355, 454, 462
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 3 cut(s) 43, 167, 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 202
HpyF3I CTNAG 2 cut(s) 372, 398
HpySE526I ACGT 1 cut(s) 408
Hsp92II CATG 2 cut(s) 158, 349
Kzo9I GATC 2 cut(s) 9, 430
LpnPI CCDG 7 cut(s) 53, 229, 240, 265, 267, 429, 447
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 154
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 404
MalI GATC 2 cut(s) 11, 432
MbiI CCGCTC 1 cut(s) 441
MboI GATC 2 cut(s) 9, 430
MboII GAAGA 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 4 cut(s) 5, 80, 185, 287
MnlI CCTC 6 cut(s) 39, 47, 51, 128, 351, 407
MroXI GAANNNNTTC 1 cut(s) 287
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 2 cut(s) 255, 417
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 1 cut(s) 255
MvnI CGCG 2 cut(s) 14, 89
MwoI GCNNNNNNNGC 2 cut(s) 130, 202
NciI CCSGG 1 cut(s) 417
NcoI CCATGG 1 cut(s) 345
NdeII GATC 2 cut(s) 9, 430
NlaIII CATG 2 cut(s) 158, 349
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 404
NruI TCGCGA 1 cut(s) 14
PctI GAATGC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 287
PkrI GCNGC 1 cut(s) 123
PshBI ATTAAT 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 2 cut(s) 48, 251
PstI CTGCAG 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 307
RruI TCGCGA 1 cut(s) 14
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 1 cut(s) 122
Sau3AI GATC 2 cut(s) 9, 430
Sau96I GGNCC 2 cut(s) 48, 251
ScrFI CCNGG 2 cut(s) 255, 417
SduI GDGCHC 1 cut(s) 115
SetI ASST 6 cut(s) 123, 193, 367, 373, 393, 411
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 41
SinI GGWCC 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 4 cut(s) 5, 80, 185, 287
SsiI CCGC 3 cut(s) 89, 402, 441
StyD4I CCNGG 2 cut(s) 253, 415
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 4 cut(s) 342, 355, 454, 462
TaiI ACGT 1 cut(s) 411
TaqI TCGA 2 cut(s) 291, 456
TasI AATT 4 cut(s) 5, 80, 185, 287
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 2 cut(s) 360, 457
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 1 cut(s) 121
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 198, 401
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 360, 457
VpaK11BI GGWCC 1 cut(s) 48
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.