FvH4_5g34470

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016644
Physical Location & Seq
Reverse (-)
25117098 .. 25121651
4554 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34470.t1

Sequence Viewer

Length: 2946 bp
ATGAACAAATTGCACTCGAAGTACTTGACCGTTCACCACAAAGCTCAGCAATTCGAGCAAAGAGTCTTAACCCATTTTGTCCATAGCATTGTGGATGCAACCCAGTCTAAGTTCGAATCCAATCTGCTATTAAAGAGGTTATCCGTTTCTGGTCGAGTCGATGATGCCCGCCAAGTGTTCGATGAAATGCCGAGCAGAGATGAGTTCAGTTGGAATACAATGATAGCTGCTTATGCCAATTCAGGAAGACTGACTGAAGCTAAACAAGTGTTTGATGCCACCCCAAGTAAGACTGCCATCACTTGGTCCTCACTGATATCTGGGTACTGCCGAAGTGAGTGTGGAAGTGAAGCTTTCCGGTTGTTTTGGCAAATGCAGCTTGAGGGGGAAAGGCCGAGTCTTTACACATTGGGGAGTTTGTTGAGGTTGTGTTCGAAATTGGGTTTGCTCCAAAGAGGTGAAATGGTGCATGGTTACATGGTAAAAACACGGTTTGATAGTAATGTTGATGTTGTTACTGGTTTGGTTGACATGTATGCAAAATGCAAGCGCATTTTTGAAGCCGGGTGTCTGTTTGAGAGTTTGAGGAATAGGAGAAATCATGTGGTGTGGACTGTTATGCTTACAGGGTACTCTCATAATGGGGATGGCTTGAAGGCAATGGAGTTTTTTCGTGACATGCAAGTGCAAGGGGTTGAATCCAATCAGTTCACATTCCCTAGTGTATTGACTGCTTCTGCATCGGTTTTAGCTCTTGGTTTTGGGGCTCAGGTGCACGGATGCATTGTACAGAGTGGTTTTGGGCCTAATGTGTTTGTTCAAAGTGCATTAGTTGACATGTACGTGAAATGCGGAGATCATATCAGTGCAAGAAAGGTGCTAAGAAGCATGGAGGTTGACGATGTTGTTTCTTGGAACTCGATGATTGTTGGGTGTGTAAGGCAGGGGTTTATAAGAGAAGCTTTGTCTTTGTTTAGAGAAATGCGTTCAAGAGAACTGAAAATTGATGATTATACATATCCATCTGTTCTGAATTCATTTGCTGCGCTGAAGGAGGTTAAAAACGCAGTGGCTATTCATTGTCTGATAGTGAAGACTGGATTTGAGGTTTATCAACTTGTAGGTAATGCTCTTGTTGATATGTATGCTAAACTGGGAAATATAGAATTTGCAGTTGAGATGTTTAGGTATATGCCAGACAAAGATGTTATCTCTTGGACCTCCCTGGTCACAGGCTATGCACAGAATGGCTCTCATGAAAAAGCACTTAAATTATTCTGTGAGATGAGGGATGCAGGGATCTATCCTGACCACTTTATCATTGCTAGCATTTTGAGTGCCTGTGCAGAACTGACACTTTTGGAGTTTGGCCAACAAATTCATGCAAACTTCATCAAGTCTGGCCTTCAAGCATCGTTATCAGTAGATAATTCTTTTCTAACATTGTATGCAAAATGTGGGTGTCTAGAAGAGGCTCTTAGAGTATTTGATTCTATGCAAGTTCAAAATGTGATTACTTGGACAGCTTTAATTGTTGGTTATGCCCAGAATGGTAGAGGAAAGGAGTCTCTGAAGTTCTACAATCAAATGCTTGCAACTGGTACCCAACCGGACTTCATTACTTTTATTGGCTTGTTATTTGCTTGTAGCCATGCTGGTCTTTTAGAAAAAGGTCAGTACTACTTTGAATCGATGAATAGGGTTTACGGAATAAAACCAGGTCCTGAGCATTATGCGTGTATGATTGACCTCTTGGGGCGCTCAGGGAAACTTAACGAGGCTGAGGCATTAGTTGATGAAATGGCTGTAGAACCAGACGGAACTGTATGGAAAGCACTACTAGCTGCATGTAGAGTTCATGGGAAAATAGAGCTGGGAGAGAGGGCAGCGGAGAACCTATTTATATTGGAGCCCAAGAATGCTGTGCCTTATGTTCAGTTATCTAATATGTATTCCGCAGCTGGTAGATGGGAAGATGCAGCAAGAATCAGAAGACTGATGAAATCAAAGGGAATTCTTAAGGAGCCTGGATGTAGTTGGATTGAGATGAATGGCCAAGTTCATACATTTATGTCTGAAGACAGAAGCCATTCATGGACTACTGAAGTATATTCAAAAATTGACGAGATCATGATGTTCATCAAGGAAGCTGGATACGTGCCAGATATGAATTTTGCACTTCATGACATGGATAAAGAGGGGAAGGAGCTTGGCCTAGCATATCACAGTGAGAAGTTGGCTGTTGCTTTTGGTCTTCTCACCACATCACCAGGGGCACCAATTCGAATCTTTAAGAATCTCCGGAAGGGAAGTGTTCCTGTGGTGACTACTGGTAGGAAATTCATCCGCCAACTGTTTTTCGTTATTGGTGGGAAATCAATTGGCTGGTTCGTGGTGTCACACTTCAAAGCCTACGCAGTGAAGTTTCTATTTAACTTCCTAGTGAGTTTTCTTGGAGCTCTAGGGAGTACTATGCTACAGGCATATGGCCGTCTAGAATTAAAAACGGCACCAGTTCATGCTCCTGCTGAGTGGTATCTGGAAAATGTCTTGCCCACAATGCATAGGTCACTCACAGATGAGGAACTCAGAATTCAGGGGCGTTGCCCGTTGAGGCTTGAAGAAATTGTATTGTTGCTAGCAGCTCTTGTCTTCAACAATAGCACACGGCTTTATCTTGCGTCCCACAAGGTTTATGGCGGAGAAGCAAGGATCACAACTCGCAGAAGATTATTTCCACTAATGGAGGATAAGAAGAGCCTTACTTCAGCCGAGCAAAGGGCCAAGGTTGAAGGCAAGGCTTCTCTGTCAGCTGCAGTTGACTATTATGTGAGCATGCAAAGTGATATCTTCATTTCTGCTTCTCCGGGAAACATGCACAATGCACTGGTAATACTTATCAAACTCTCAATACTCAGTAACGGTTTCTGTTCTACAAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR

Protein Analysis

982

Amino Acids

110.0

Weight (kDa)

8.42

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 98 - 145 1.2e-07 PPR repeat family
PPR PF01535 100 - 129 1.7e-06 PPR repeat
PPR_2 PF13041 200 - 245 1.6e-08 PPR repeat family
PPR PF01535 202 - 232 5.5e-06 PPR repeat
PPR_2 PF13041 301 - 348 4.5e-12 PPR repeat family
PPR PF01535 303 - 331 1.8e-08 PPR repeat
PPR_2 PF13041 401 - 448 2.2e-09 PPR repeat family
PPR PF01535 404 - 434 2e-08 PPR repeat
PPR_2 PF13041 503 - 550 5.7e-09 PPR repeat family
E_motif PF20431 620 - 682 1.7e-20 E motif
DYW_deaminase PF14432 718 - 768 8.4e-14 DYW family of nucleic acid deaminases
O-FucT PF10250 860 - 962 2.7e-15 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016644)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61170
fragaria_vesca FvH4_5g34470
malus_domestica MD07G1126900.v1.1
prunus_persica Prupe.2G157100_v2.0.a1
pyrus_communis pycom07g12240
rosa_chinensis RchiOBHm_Chr7g0235091
rosa_multiflora Rmu_sc0002239.1_g000010
rosa_roxburghii Rroxscaffold_3G00226310
rosa_rugosa Rorug07G0287300 Rorug07G0287400
rosa_samantha Rh7AG442400 Rh7BG415000 Rh7CG463300
rosa_wichuraiana Rw7G036780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
Acc65I GGTACC 1 cut(s) 1601
AccB1I GGYRCC 3 cut(s) 1601, 2275, 2509
AccB7I CCANNNNNTGG 1 cut(s) 303
AccIII TCCGGA 1 cut(s) 2301
AciI CCGC 6 cut(s) 169, 852, 1889, 1956, 2347, 2700
AclWI GGATC 2 cut(s) 1307, 2720
AcoI YGGCCR 3 cut(s) 1369, 2053, 2488
AcsI RAATTY 7 cut(s) 1033, 1166, 1377, 2013, 2170, 2339, 2592
AcuI CTGAAG 6 cut(s) 276, 1070, 1592, 2097, 2124, 2751
AfaI GTAC 8 cut(s) 23, 326, 632, 789, 842, 1603, 1679, 2470
AfiI CCNNNNNNNGG 2 cut(s) 303, 2778
AflII CTTAAG 1 cut(s) 2018
AflIII ACRYGT 2 cut(s) 531, 837
AjnI CCWGG 4 cut(s) 1224, 1717, 2026, 2269
Alw21I GWGCWC 2 cut(s) 777, 2461
Alw26I GTCTC 1 cut(s) 1572
Alw44I GTGCAC 1 cut(s) 773
AlwI GGATC 2 cut(s) 1307, 2720
AlwNI CAGNNNCTG 1 cut(s) 1724
Aor13HI TCCGGA 1 cut(s) 2301
AoxI GGCC 8 cut(s) 392, 803, 1369, 1402, 2053, 2212, 2488, 2781
ApaLI GTGCAC 1 cut(s) 773
ApeKI GCWGC 9 cut(s) 227, 376, 1043, 1844, 1886, 1958, 1979, 2642, 2813
ApoI RAATTY 7 cut(s) 1033, 1166, 1377, 2013, 2170, 2339, 2592
Asp700I GAANNNNTTC 1 cut(s) 2089
Asp718I GGTACC 1 cut(s) 1601
AspLEI GCGC 3 cut(s) 552, 1048, 1761
AspS9I GGNCC 5 cut(s) 306, 803, 1218, 1721, 2781
AsuC2I CCSGG 2 cut(s) 565, 2868
AsuHPI GGTGA 5 cut(s) 26, 470, 2251, 2259, 2335
AsuII TTCGAA 3 cut(s) 114, 434, 2284
AsuNHI GCTAGC 2 cut(s) 1325, 2638
AvaII GGWCC 3 cut(s) 306, 1218, 1721
BaeGI GKGCMC 2 cut(s) 777, 2278
BalI TGGCCA 2 cut(s) 1371, 2055
BanI GGYRCC 3 cut(s) 1601, 2275, 2509
BanII GRGCYC 3 cut(s) 769, 1914, 2461
BbsI GAAGAC 6 cut(s) 253, 1100, 1999, 2085, 2246, 2644
Bbv12I GWGCWC 2 cut(s) 777, 2461
BbvCI CCTCAGC 1 cut(s) 1782
BbvI GCAGC 9 cut(s) 214, 388, 1030, 1831, 1898, 1970, 1991, 2654, 2800
BccI CCATC 4 cut(s) 305, 641, 1030, 1962
BceAI ACGGC 3 cut(s) 2475, 2523, 2684
BciT130I CCWGG 4 cut(s) 1226, 1719, 2028, 2271
BciVI GTATCC 1 cut(s) 2147
BcnI CCSGG 2 cut(s) 565, 2868
BcoDI GTCTC 1 cut(s) 1572
BfaI CTAG 9 cut(s) 720, 1326, 1466, 1841, 2216, 2441, 2462, 2495, 2639
BfmI CTRYAG 3 cut(s) 1806, 2477, 2814
BfoI RGCGCY 1 cut(s) 1762
BfrI CTTAAG 1 cut(s) 2018
BfuI GTATCC 1 cut(s) 2147
BisI GCNGC 9 cut(s) 228, 377, 1044, 1845, 1887, 1959, 1980, 2643, 2814
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 9 cut(s) 229, 378, 1045, 1846, 1888, 1960, 1981, 2644, 2815
BmcAI AGTACT 3 cut(s) 23, 1679, 2470
Bme1390I CCNGG 6 cut(s) 565, 1226, 1719, 2028, 2271, 2868
Bme18I GGWCC 3 cut(s) 306, 1218, 1721
BmgT120I GGNCC 5 cut(s) 306, 803, 1218, 1721, 2781
BmiI GGNNCC 5 cut(s) 1603, 1911, 2025, 2277, 2511
BmrFI CCNGG 6 cut(s) 565, 1226, 1719, 2028, 2271, 2868
BmrI ACTGGG 2 cut(s) 97, 1163
BmsI GCATC 8 cut(s) 85, 154, 265, 749, 770, 1282, 1421, 1966
BmtI GCTAGC 2 cut(s) 1329, 2642
BmuI ACTGGG 2 cut(s) 97, 1163
BpiI GAAGAC 6 cut(s) 253, 1100, 1999, 2085, 2246, 2644
Bpu10I CCTNAGC 4 cut(s) 768, 1725, 1762, 1782
Bpu1102I GCTNAGC 1 cut(s) 45
Bpu14I TTCGAA 3 cut(s) 114, 434, 2284
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 2 cut(s) 565, 2868
Bsa29I ATCGAT 1 cut(s) 1691
BsaAI YACGTR 2 cut(s) 844, 2158
BsaBI GATNNNNATC 1 cut(s) 2138
BsaJI CCNNGG 3 cut(s) 1224, 2270, 2784
BsaWI WCCGGW 3 cut(s) 357, 1609, 2301
Bsc4I CCNNNNNNNGG 2 cut(s) 303, 2778
Bse1I ACTGG 8 cut(s) 103, 523, 1102, 1158, 1603, 2335, 2513, 2892
Bse3DI GCAATG 2 cut(s) 666, 1320
Bse8I GATNNNNATC 1 cut(s) 2138
BseAI TCCGGA 1 cut(s) 2301
BseBI CCWGG 4 cut(s) 1226, 1719, 2028, 2271
BseCI ATCGAT 1 cut(s) 1691
BseDI CCNNGG 3 cut(s) 1224, 2270, 2784
BseGI GGATG 6 cut(s) 100, 652, 785, 1297, 2036, 2343
BseJI GATNNNNATC 1 cut(s) 2138
BseLI CCNNNNNNNGG 2 cut(s) 303, 2778
BseMI GCAATG 2 cut(s) 666, 1320
BseMII CTCAG 8 cut(s) 59, 782, 1716, 1773, 1776, 2520, 2602, 2929
BseNI ACTGG 8 cut(s) 103, 523, 1102, 1158, 1603, 2335, 2513, 2892
BseSI GKGCMC 2 cut(s) 777, 2278
BseXI GCAGC 9 cut(s) 214, 388, 1030, 1831, 1898, 1970, 1991, 2654, 2800
BseYI CCCAGC 1 cut(s) 1873
BsgI GTGCAG 1 cut(s) 1365
BshFI GGCC 8 cut(s) 394, 805, 1371, 1404, 2055, 2214, 2490, 2783
BshNI GGYRCC 3 cut(s) 1601, 2275, 2509
BshVI ATCGAT 1 cut(s) 1691
BsiHKAI GWGCWC 2 cut(s) 777, 2461
BsiSI CCGG 5 cut(s) 358, 564, 1610, 2302, 2867
BslFI GGGAC 1 cut(s) 2668
BslI CCNNNNNNNGG 2 cut(s) 303, 2778
BsmAI GTCTC 1 cut(s) 1572
BsmFI GGGAC 1 cut(s) 2668
BsmI GAATGC 1 cut(s) 1924
BsnI GGCC 8 cut(s) 394, 805, 1371, 1404, 2055, 2214, 2490, 2783
Bsp119I TTCGAA 3 cut(s) 114, 434, 2284
Bsp1286I GDGCHC 5 cut(s) 769, 777, 1914, 2278, 2461
Bsp13I TCCGGA 1 cut(s) 2301
Bsp1407I TGTACA 1 cut(s) 787
Bsp143I GATC 4 cut(s) 856, 1299, 2127, 2712
Bsp1720I GCTNAGC 1 cut(s) 45
BspACI CCGC 6 cut(s) 169, 852, 1889, 1956, 2347, 2700
BspANI GGCC 8 cut(s) 394, 805, 1371, 1404, 2055, 2214, 2490, 2783
BspCNI CTCAG 8 cut(s) 58, 781, 1717, 1774, 1775, 2521, 2601, 2928
BspDI ATCGAT 1 cut(s) 1691
BspEI TCCGGA 1 cut(s) 2301
BspHI TCATGA 3 cut(s) 1255, 2130, 2182
BspLI GGNNCC 5 cut(s) 1603, 1911, 2025, 2277, 2511
BspMAI CTGCAG 1 cut(s) 2818
BspOI GCTAGC 2 cut(s) 1329, 2642
BspPI GGATC 2 cut(s) 1307, 2720
BspQI GCTCTTC 1 cut(s) 2750
BspT104I TTCGAA 3 cut(s) 114, 434, 2284
BspT107I GGYRCC 3 cut(s) 1601, 2275, 2509
BspTI CTTAAG 1 cut(s) 2018
BsrDI GCAATG 2 cut(s) 666, 1320
BsrGI TGTACA 1 cut(s) 787
BsrI ACTGG 8 cut(s) 103, 523, 1102, 1158, 1603, 2335, 2513, 2892
BssECI CCNNGG 3 cut(s) 1224, 2270, 2784
BssMI GATC 4 cut(s) 856, 1299, 2127, 2712
BssT1I CCWWGG 1 cut(s) 2784
Bst2UI CCWGG 4 cut(s) 1226, 1719, 2028, 2271
Bst4CI ACNGT 7 cut(s) 31, 492, 616, 1825, 2228, 2355, 2924
Bst6I CTCTTC 2 cut(s) 1464, 2750
BstAFI CTTAAG 1 cut(s) 2018
BstAUI TGTACA 1 cut(s) 787
BstBAI YACGTR 2 cut(s) 844, 2158
BstBI TTCGAA 3 cut(s) 114, 434, 2284
BstC8I GCNNGC 6 cut(s) 169, 548, 1327, 1593, 2640, 2837
BstF5I GGATG 6 cut(s) 100, 652, 785, 1297, 2036, 2343
BstH2I RGCGCY 1 cut(s) 1762
BstHHI GCGC 3 cut(s) 552, 1048, 1761
BstKTI GATC 4 cut(s) 859, 1302, 2130, 2715
BstMAI GTCTC 1 cut(s) 1572
BstMBI GATC 4 cut(s) 856, 1299, 2127, 2712
BstMWI GCNNNNNNNGC 5 cut(s) 55, 233, 376, 1841, 2560
BstNI CCWGG 4 cut(s) 1226, 1719, 2028, 2271
BstNSI RCATGY 6 cut(s) 535, 682, 841, 1851, 2839, 2878
BstSCI CCNGG 6 cut(s) 563, 1224, 1717, 2026, 2269, 2866
BstSFI CTRYAG 3 cut(s) 1806, 2477, 2814
BstSLI GKGCMC 2 cut(s) 777, 2278
BstV1I GCAGC 9 cut(s) 214, 388, 1030, 1831, 1898, 1970, 1991, 2654, 2800
BstV2I GAAGAC 6 cut(s) 253, 1100, 1999, 2085, 2246, 2644
BstX2I RGATCY 1 cut(s) 1299
BstYI RGATCY 1 cut(s) 1299
Bsu15I ATCGAT 1 cut(s) 1691
BsuI GTATCC 1 cut(s) 2147
BsuRI GGCC 8 cut(s) 394, 805, 1371, 1404, 2055, 2214, 2490, 2783
BsuTUI ATCGAT 1 cut(s) 1691
BtsCI GGATG 6 cut(s) 100, 652, 785, 1297, 2036, 2343
BtsI GCAGTG 2 cut(s) 1074, 2424
BtsIMutI CAGTG 6 cut(s) 311, 871, 1074, 2233, 2424, 2885
Cac8I GCNNGC 6 cut(s) 169, 548, 1327, 1593, 2640, 2837
CaiI CAGNNNCTG 1 cut(s) 1724
CciI TCATGA 3 cut(s) 1255, 2130, 2182
CfoI GCGC 3 cut(s) 552, 1048, 1761
Cfr13I GGNCC 5 cut(s) 306, 803, 1218, 1721, 2781
ClaI ATCGAT 1 cut(s) 1691
CseI GACGC 1 cut(s) 2670
CsiI ACCWGGT 1 cut(s) 1717
Csp6I GTAC 8 cut(s) 22, 325, 631, 788, 841, 1602, 1678, 2469
CviQI GTAC 8 cut(s) 22, 325, 631, 788, 841, 1602, 1678, 2469
DpnI GATC 4 cut(s) 858, 1301, 2129, 2714
DpnII GATC 4 cut(s) 856, 1299, 2127, 2712
EaeI YGGCCR 3 cut(s) 1369, 2053, 2488
Eam1104I CTCTTC 2 cut(s) 1464, 2750
EarI CTCTTC 2 cut(s) 1464, 2750
EciI GGCGGA 2 cut(s) 2336, 2715
Ecl136II GAGCTC 1 cut(s) 2459
Eco130I CCWWGG 1 cut(s) 2784
Eco24I GRGCYC 3 cut(s) 769, 1914, 2461
Eco32I GATATC 2 cut(s) 318, 2848
Eco47I GGWCC 3 cut(s) 306, 1218, 1721
Eco53kI GAGCTC 1 cut(s) 2459
Eco57I CTGAAG 6 cut(s) 276, 1070, 1592, 2097, 2124, 2751
EcoICRI GAGCTC 1 cut(s) 2459
EcoO109I RGGNCCY 1 cut(s) 1721
EcoRI GAATTC 3 cut(s) 1033, 2013, 2592
EcoRII CCWGG 4 cut(s) 1224, 1717, 2026, 2269
EcoRV GATATC 2 cut(s) 318, 2848
EcoT14I CCWWGG 1 cut(s) 2784
EcoT22I ATGCAT 2 cut(s) 785, 2565
EcoT38I GRGCYC 3 cut(s) 769, 1914, 2461
ErhI CCWWGG 1 cut(s) 2784
FalI AAGNNNNNCTT 6 cut(s) 337, 369, 946, 978, 1461, 1493
FaqI GGGAC 1 cut(s) 2668
FauI CCCGC 1 cut(s) 176
FauNDI CATATG 1 cut(s) 2485
Fnu4HI GCNGC 9 cut(s) 228, 377, 1044, 1845, 1887, 1959, 1980, 2643, 2814
FokI GGATG 6 cut(s) 107, 659, 792, 1304, 2043, 2330
FriOI GRGCYC 3 cut(s) 769, 1914, 2461
Fsp4HI GCNGC 9 cut(s) 228, 377, 1044, 1845, 1887, 1959, 1980, 2643, 2814
FspBI CTAG 9 cut(s) 720, 1326, 1466, 1841, 2216, 2441, 2462, 2495, 2639
GlaI GCGC 3 cut(s) 551, 1047, 1760
GluI GCNGC 9 cut(s) 228, 377, 1044, 1845, 1887, 1959, 1980, 2643, 2814
GsaI CCCAGC 1 cut(s) 1877
HaeII RGCGCY 1 cut(s) 1762
HaeIII GGCC 8 cut(s) 394, 805, 1371, 1404, 2055, 2214, 2490, 2783
HapII CCGG 5 cut(s) 358, 564, 1610, 2302, 2867
HgaI GACGC 1 cut(s) 2670
HhaI GCGC 3 cut(s) 552, 1048, 1761
Hin6I GCGC 3 cut(s) 550, 1046, 1759
HinP1I GCGC 3 cut(s) 550, 1046, 1759
HincII GTYRAC 4 cut(s) 529, 835, 898, 2821
HindII GTYRAC 4 cut(s) 529, 835, 898, 2821
HindIII AAGCTT 2 cut(s) 351, 960
HpaII CCGG 5 cut(s) 358, 564, 1610, 2302, 2867
HphI GGTGA 5 cut(s) 26, 470, 2251, 2259, 2335
Hpy166II GTNNAC 9 cut(s) 34, 529, 612, 711, 775, 835, 898, 1705, 2821
Hpy188I TCNGA 6 cut(s) 1032, 1086, 1572, 1991, 2077, 2591
Hpy8I GTNNAC 9 cut(s) 34, 529, 612, 711, 775, 835, 898, 1705, 2821
HpyAV CCTTC 6 cut(s) 649, 1045, 1415, 2197, 2299, 2786
HpyCH4III ACNGT 7 cut(s) 31, 492, 616, 1825, 2228, 2355, 2924
HpyCH4IV ACGT 2 cut(s) 843, 2157
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 233, 376, 1841, 2560
HpySE526I ACGT 2 cut(s) 843, 2157
HspAI GCGC 3 cut(s) 550, 1046, 1759
Kpn2I TCCGGA 1 cut(s) 2301
KpnI GGTACC 1 cut(s) 1605
Kzo9I GATC 4 cut(s) 856, 1299, 2127, 2712
LguI GCTCTTC 1 cut(s) 2750
LmnI GCTCC 6 cut(s) 453, 1909, 2023, 2206, 2456, 2527
Lsp1109I GCAGC 9 cut(s) 214, 388, 1030, 1831, 1898, 1970, 1991, 2654, 2800
LweI GCATC 8 cut(s) 85, 154, 265, 749, 770, 1282, 1421, 1966
MabI ACCWGGT 1 cut(s) 1717
MaeI CTAG 9 cut(s) 720, 1326, 1466, 1841, 2216, 2441, 2462, 2495, 2639
MaeII ACGT 2 cut(s) 843, 2157
MaeIII GTNAC 8 cut(s) 473, 514, 674, 1228, 2323, 2397, 2568, 2918
MalI GATC 4 cut(s) 858, 1301, 2129, 2714
MboI GATC 4 cut(s) 856, 1299, 2127, 2712
MfeI CAATTG 1 cut(s) 2379
MflI RGATCY 1 cut(s) 1299
MhlI GDGCHC 5 cut(s) 769, 777, 1914, 2278, 2461
MlsI TGGCCA 2 cut(s) 1371, 2055
MluNI TGGCCA 2 cut(s) 1371, 2055
MlyI GAGTC 4 cut(s) 72, 165, 406, 1574
MmeI TCCRAC 2 cut(s) 191, 2018
Mox20I TGGCCA 2 cut(s) 1371, 2055
Mph1103I ATGCAT 2 cut(s) 785, 2565
MroI TCCGGA 1 cut(s) 2301
MroXI GAANNNNTTC 1 cut(s) 2089
MscI TGGCCA 2 cut(s) 1371, 2055
MslI CAYNNNNRTG 3 cut(s) 683, 842, 864
Msp20I TGGCCA 2 cut(s) 1371, 2055
MspA1I CMGCKG 3 cut(s) 1889, 1961, 2813
MspCI CTTAAG 1 cut(s) 2018
MspI CCGG 5 cut(s) 358, 564, 1610, 2302, 2867
MspR9I CCNGG 6 cut(s) 565, 1226, 1719, 2028, 2271, 2868
MunI CAATTG 1 cut(s) 2379
Mva1269I GAATGC 1 cut(s) 1924
MvaI CCWGG 4 cut(s) 1226, 1719, 2028, 2271
MwoI GCNNNNNNNGC 5 cut(s) 55, 233, 376, 1841, 2560
NciI CCSGG 2 cut(s) 565, 2868
NdeI CATATG 1 cut(s) 2485
NdeII GATC 4 cut(s) 856, 1299, 2127, 2712
NheI GCTAGC 2 cut(s) 1325, 2638
NlaIV GGNNCC 5 cut(s) 1603, 1911, 2025, 2277, 2511
NmeAIII GCCGAG 3 cut(s) 216, 420, 2797
NmuCI GTSAC 5 cut(s) 674, 1228, 2323, 2397, 2568
NsiI ATGCAT 2 cut(s) 785, 2565
NspI RCATGY 6 cut(s) 535, 682, 841, 1851, 2839, 2878
NspV TTCGAA 3 cut(s) 114, 434, 2284
PaeI GCATGC 1 cut(s) 2839
PagI TCATGA 3 cut(s) 1255, 2130, 2182
PciI ACATGT 2 cut(s) 531, 837
PciSI GCTCTTC 1 cut(s) 2750
PctI GAATGC 1 cut(s) 1924
PdmI GAANNNNTTC 1 cut(s) 2089
PfeI GAWTC 7 cut(s) 116, 698, 1490, 1688, 1986, 2286, 2296
PflMI CCANNNNNTGG 1 cut(s) 303
PfoI TCCNGGA 1 cut(s) 2866
PkrI GCNGC 9 cut(s) 229, 378, 1045, 1846, 1888, 1960, 1981, 2644, 2815
PleI GAGTC 4 cut(s) 71, 164, 405, 1573
PpsI GAGTC 4 cut(s) 71, 164, 405, 1573
Ppu21I YACGTR 2 cut(s) 844, 2158
PpuMI RGGWCCY 1 cut(s) 1721
PscI ACATGT 2 cut(s) 531, 837
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 2461
Psp5II RGGWCCY 1 cut(s) 1721
Psp6I CCWGG 4 cut(s) 1224, 1717, 2026, 2269
PspFI CCCAGC 1 cut(s) 1873
PspGI CCWGG 4 cut(s) 1224, 1717, 2026, 2269
PspN4I GGNNCC 5 cut(s) 1603, 1911, 2025, 2277, 2511
PspPI GGNCC 5 cut(s) 306, 803, 1218, 1721, 2781
PspPPI RGGWCCY 1 cut(s) 1721
PstI CTGCAG 1 cut(s) 2818
PstNI CAGNNNCTG 1 cut(s) 1724
PsuI RGATCY 1 cut(s) 1299
PvuII CAGCTG 2 cut(s) 1961, 2813
RsaI GTAC 8 cut(s) 23, 326, 632, 789, 842, 1603, 1679, 2470
RsaNI GTAC 8 cut(s) 22, 325, 631, 788, 841, 1602, 1678, 2469
RseI CAYNNNNRTG 3 cut(s) 683, 842, 864
SacI GAGCTC 1 cut(s) 2461
SapI GCTCTTC 1 cut(s) 2750
SatI GCNGC 9 cut(s) 228, 377, 1044, 1845, 1887, 1959, 1980, 2643, 2814
Sau3AI GATC 4 cut(s) 856, 1299, 2127, 2712
Sau96I GGNCC 5 cut(s) 306, 803, 1218, 1721, 2781
ScaI AGTACT 3 cut(s) 23, 1679, 2470
SchI GAGTC 4 cut(s) 72, 165, 406, 1574
ScrFI CCNGG 6 cut(s) 565, 1226, 1719, 2028, 2271, 2868
SduI GDGCHC 5 cut(s) 769, 777, 1914, 2278, 2461
SexAI ACCWGGT 1 cut(s) 1717
SfaNI GCATC 8 cut(s) 85, 154, 265, 749, 770, 1282, 1421, 1966
SfcI CTRYAG 3 cut(s) 1806, 2477, 2814
SfuI TTCGAA 3 cut(s) 114, 434, 2284
SinI GGWCC 3 cut(s) 306, 1218, 1721
SmiMI CAYNNNNRTG 3 cut(s) 683, 842, 864
SmlI CTYRAG 2 cut(s) 380, 2018
SmoI CTYRAG 2 cut(s) 380, 2018
SphI GCATGC 1 cut(s) 2839
SsiI CCGC 6 cut(s) 169, 852, 1889, 1956, 2347, 2700
SspMI CTAG 9 cut(s) 720, 1326, 1466, 1841, 2216, 2441, 2462, 2495, 2639
SstI GAGCTC 1 cut(s) 2461
StyD4I CCNGG 6 cut(s) 563, 1224, 1717, 2026, 2269, 2866
StyI CCWWGG 1 cut(s) 2784
TaaI ACNGT 7 cut(s) 31, 492, 616, 1825, 2228, 2355, 2924
TaiI ACGT 2 cut(s) 846, 2160
TatI WGTACW 4 cut(s) 21, 787, 1677, 2468
TfiI GAWTC 7 cut(s) 116, 698, 1490, 1688, 1986, 2286, 2296
TscAI CASTG 6 cut(s) 318, 871, 1074, 2233, 2424, 2892
TseFI GTSAC 5 cut(s) 674, 1228, 2323, 2397, 2568
TseI GCWGC 9 cut(s) 227, 376, 1043, 1844, 1886, 1958, 1979, 2642, 2813
Tsp45I GTSAC 5 cut(s) 674, 1228, 2323, 2397, 2568
TspGWI ACGGA 4 cut(s) 133, 792, 1722, 1833
TspRI CASTG 6 cut(s) 318, 871, 1074, 2233, 2424, 2892
Van91I CCANNNNNTGG 1 cut(s) 303
Vha464I CTTAAG 1 cut(s) 2018
VneI GTGCAC 1 cut(s) 773
VpaK11BI GGWCC 3 cut(s) 306, 1218, 1721
XapI RAATTY 7 cut(s) 1033, 1166, 1377, 2013, 2170, 2339, 2592
XbaI TCTAGA 2 cut(s) 1465, 2494
XceI RCATGY 6 cut(s) 535, 682, 841, 1851, 2839, 2878
XcmI CCANNNNNNNNNTGG 1 cut(s) 2693
XmnI GAANNNNTTC 1 cut(s) 2089
XspI CTAG 9 cut(s) 720, 1326, 1466, 1841, 2216, 2441, 2462, 2495, 2639
ZrmI AGTACT 3 cut(s) 23, 1679, 2470
Zsp2I ATGCAT 2 cut(s) 785, 2565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.