FvH4_5g35310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014751
Physical Location & Seq
Forward (+)
25860087 .. 25862472
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35310.t1

Sequence Viewer

Length: 627 bp
ATGTCCCCCACCTTCATCTGCTTCCTACTAGTTGTTGATATCTTTGTTGGTCAATATGTTCTCCAAACCTGGAGAGGACGGGAGAACCTCCTTTCACACTTCCTTGTTTTCTTCATGGGCAATGACTACGCCGTTTACGCCATCATTTACCCAGATATCCAACAAATTGTACCACCACGTCAGCATAAGATTACTCTGACCTTGTCCAATTTTGCCGCTGTGCTCGCTGCTAAACTTCCCTATATTCTTGCTTTTGTGGACCTTGAGTTGCGTCCAAGTCTCTTCACTGTCCGCGGATTGGCTTCTTTCATCATTTCTTTCTTGACAGAAGCAGACATCTTTATCTTCCATTGGCGTCCAGGACGTTGGCTGCGGGTGGGATTGTATCCACAGTTGTATTGGCAGTCGTGTCTTCGGTTTCTGGTTTTTGTTGGTCTAATTGTATTTGGAGGTGCCAACATGATTGACAAGACATGGCATAGTGCTTTGGTCTTTCACAAGCATGTCTTGATCCCTTTGAGTCTCTGTGTTTTTGGTTATATCCGCTTCTGCGAAAAATATCGTAGATTGAGAAGGGTGATGAAAAAGGCAATGACTGTGATGCCAATAGATGAAAAGATTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

24.33

Weight (kDa)

9.59

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 452
AccII CGCG 1 cut(s) 294
AciI CCGC 5 cut(s) 216, 292, 294, 373, 544
AclWI GGATC 1 cut(s) 505
AcyI GRCGYC 1 cut(s) 355
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 298
AhlI ACTAGT 1 cut(s) 28
AjiI CACGTC 1 cut(s) 179
AjnI CCWGG 2 cut(s) 68, 358
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 284, 527
AlwI GGATC 1 cut(s) 505
ApeKI GCWGC 2 cut(s) 227, 370
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 452
BbsI GAAGAC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 214, 357
BccI CCATC 1 cut(s) 149
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 2 cut(s) 70, 360
BciVI GTATCC 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 284, 527
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 1 cut(s) 29
BfuI GTATCC 1 cut(s) 396
BisI GCNGC 3 cut(s) 216, 228, 371
BlsI GCNGC 3 cut(s) 217, 229, 372
Bme1390I CCNGG 2 cut(s) 70, 360
Bme18I GGWCC 1 cut(s) 259
BmgBI CACGTC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 454
BmrFI CCNGG 2 cut(s) 70, 360
BmsI GCATC 1 cut(s) 591
BpiI GAAGAC 1 cut(s) 404
BpmI CTGGAG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 284
BsaHI GRCGYC 1 cut(s) 355
BsaJI CCNNGG 1 cut(s) 292
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse3DI GCAATG 2 cut(s) 127, 597
BseBI CCWGG 2 cut(s) 70, 360
BseDI CCNNGG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 2 cut(s) 127, 597
BseXI GCAGC 2 cut(s) 214, 357
Bsh1236I CGCG 1 cut(s) 294
BshNI GGYRCC 1 cut(s) 452
BsiHKAI GWGCWC 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 2 cut(s) 284, 527
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 1 cut(s) 510
BspACI CCGC 5 cut(s) 216, 292, 294, 373, 544
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 454
BspPI GGATC 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 127, 597
BssECI CCNNGG 1 cut(s) 292
BssMI GATC 1 cut(s) 510
BssNI GRCGYC 1 cut(s) 355
Bst2UI CCWGG 2 cut(s) 70, 360
Bst4CI ACNGT 3 cut(s) 289, 393, 598
Bst6I CTCTTC 1 cut(s) 287
BstACI GRCGYC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 225
BstDSI CCRYGG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 1 cut(s) 513
BstMAI GTCTC 2 cut(s) 284, 527
BstMBI GATC 1 cut(s) 510
BstMWI GCNNNNNNNGC 2 cut(s) 137, 224
BstNI CCWGG 2 cut(s) 70, 360
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 2 cut(s) 68, 358
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 214, 357
BstV2I GAAGAC 1 cut(s) 404
BstXI CCANNNNNNTGG 1 cut(s) 366
BsuI GTATCC 1 cut(s) 396
BtgI CCRYGG 1 cut(s) 292
BtrI CACGTC 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 259
Cfr42I CCGCGG 1 cut(s) 295
CseI GACGC 2 cut(s) 260, 344
Csp6I GTAC 1 cut(s) 170
CviAII CATG 4 cut(s) 115, 460, 474, 503
CviJI RGCY 2 cut(s) 302, 370
CviKI_1 RGCY 2 cut(s) 302, 370
CviQI GTAC 1 cut(s) 170
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
Eco32I GATATC 2 cut(s) 40, 157
Eco47I GGWCC 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 68, 358
EcoRV GATATC 2 cut(s) 40, 157
FaeI CATG 4 cut(s) 118, 463, 477, 506
FaiI YATR 9 cut(s) 57, 116, 186, 243, 461, 475, 480, 504, 540
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FatI CATG 4 cut(s) 114, 459, 473, 502
FauI CCCGC 1 cut(s) 366
Fnu4HI GCNGC 3 cut(s) 216, 228, 371
Fsp4HI GCNGC 3 cut(s) 216, 228, 371
FspBI CTAG 1 cut(s) 29
GluI GCNGC 3 cut(s) 216, 228, 371
GsuI CTGGAG 1 cut(s) 91
HgaI GACGC 2 cut(s) 260, 344
Hin1I GRCGYC 1 cut(s) 355
Hin1II CATG 4 cut(s) 118, 463, 477, 506
HinfI GANTC 1 cut(s) 520
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 2 cut(s) 136, 259
Hpy188I TCNGA 1 cut(s) 198
Hpy188III TCNNGA 2 cut(s) 322, 508
Hpy8I GTNNAC 2 cut(s) 136, 259
HpyAV CCTTC 2 cut(s) 22, 567
HpyCH4III ACNGT 3 cut(s) 289, 393, 598
HpyCH4IV ACGT 2 cut(s) 178, 364
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 224
HpySE526I ACGT 2 cut(s) 178, 364
Hsp92I GRCGYC 1 cut(s) 355
Hsp92II CATG 4 cut(s) 118, 463, 477, 506
KspI CCGCGG 1 cut(s) 295
Kzo9I GATC 1 cut(s) 510
LpnPI CCDG 6 cut(s) 55, 82, 165, 345, 372, 407
Lsp1109I GCAGC 2 cut(s) 214, 357
LweI GCATC 1 cut(s) 591
MaeI CTAG 1 cut(s) 29
MaeII ACGT 2 cut(s) 178, 364
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 4 cut(s) 103, 274, 337, 404
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 3 cut(s) 165, 208, 438
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 3 cut(s) 68, 98, 443
MslI CAYNNNNRTG 1 cut(s) 501
MspA1I CMGCKG 2 cut(s) 218, 294
MspR9I CCNGG 2 cut(s) 70, 360
MvaI CCWGG 2 cut(s) 70, 360
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 137, 224
NdeII GATC 1 cut(s) 510
NlaIII CATG 4 cut(s) 118, 463, 477, 506
NlaIV GGNNCC 1 cut(s) 454
NspI RCATGY 1 cut(s) 506
PflFI GACNNNGTC 1 cut(s) 202
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 3 cut(s) 217, 229, 372
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
Psp6I CCWGG 2 cut(s) 68, 358
PspGI CCWGG 2 cut(s) 68, 358
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 202
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
RseI CAYNNNNRTG 1 cut(s) 501
SacII CCGCGG 1 cut(s) 295
SatI GCNGC 3 cut(s) 216, 228, 371
Sau3AI GATC 1 cut(s) 510
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 2 cut(s) 70, 360
SduI GDGCHC 1 cut(s) 225
SetI ASST 8 cut(s) 14, 71, 90, 181, 203, 264, 367, 454
SfaNI GCATC 1 cut(s) 591
Sfr303I CCGCGG 1 cut(s) 295
SgrBI CCGCGG 1 cut(s) 295
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SpeI ACTAGT 1 cut(s) 28
Sse9I AATT 3 cut(s) 165, 208, 438
SsiI CCGC 5 cut(s) 216, 292, 294, 373, 544
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 2 cut(s) 68, 358
TaaI ACNGT 3 cut(s) 289, 393, 598
TaiI ACGT 2 cut(s) 181, 367
TasI AATT 3 cut(s) 165, 208, 438
TauI GCSGC 1 cut(s) 218
TscAI CASTG 1 cut(s) 292
TseI GCWGC 2 cut(s) 227, 370
TspDTI ATGAA 5 cut(s) 4, 103, 298, 596, 627
TspRI CASTG 1 cut(s) 292
Tth111I GACNNNGTC 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 259
XceI RCATGY 1 cut(s) 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 396
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.