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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G24610 AT4G24610 AT4G24610 AT4G24610 |
| fragaria_vesca | FvH4_5g35670 FvH4_5g35670 |
| malus_domestica | MD08G1194200.v1.1 MD15G1382300.v1.1 |
| prunus_persica | Prupe.1G528500_v2.0.a1 |
| pyrus_communis | pycom08g16710 pycom08g16720 pycom15g34240 |
| rosa_chinensis | RchiOBHm_Chr7g0237261 |
| rosa_laevigata | RLG00000001014 RLG00000001019 |
| rosa_multiflora | Rmu_co8497497.1_g000001 Rmu_sc0002923.1_g000046 Rmu_sc0027723.1_g000002 |
| rosa_roxburghii | Rroxscaffold_3G00224340 |
| rosa_rugosa | Rorug07G0302600 |
| rosa_samantha | Rh2BG240700 Rh7AG459400 Rh7BG429500 Rh7CG476800 Rh7DG446000 |
| rosa_wichuraiana | Rw7G038050 Rw7G038070 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2203 |
| Acc16I | TGCGCA | 1 cut(s) | 1439 |
| Acc36I | ACCTGC | 2 cut(s) | 1342, 2203 |
| AccB1I | GGYRCC | 2 cut(s) | 595, 2971 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 752, 929 |
| AccI | GTMKAC | 2 cut(s) | 1277, 1923 |
| AccII | CGCG | 1 cut(s) | 385 |
| AccIII | TCCGGA | 1 cut(s) | 117 |
| AciI | CCGC | 7 cut(s) | 317, 341, 383, 593, 1226, 2536, 2576 |
| AclWI | GGATC | 8 cut(s) | 92, 1546, 1655, 2295, 2360, 2373, 2443, 3438 |
| AcoI | YGGCCR | 2 cut(s) | 1624, 2858 |
| AcsI | RAATTY | 6 cut(s) | 164, 693, 2097, 2355, 2965, 3064 |
| AcuI | CTGAAG | 2 cut(s) | 30, 1083 |
| AcyI | GRCGYC | 1 cut(s) | 158 |
| AdeI | CACNNNGTG | 1 cut(s) | 1328 |
| AfaI | GTAC | 9 cut(s) | 407, 667, 1332, 1384, 1478, 2946, 3364, 3450, 3491 |
| AfeI | AGCGCT | 2 cut(s) | 349, 1679 |
| AjiI | CACGTC | 2 cut(s) | 1355, 1555 |
| AjnI | CCWGG | 6 cut(s) | 217, 1534, 2280, 3160, 3387, 3582 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2951, 2983 |
| AleI | CACNNNNGTG | 1 cut(s) | 437 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2111, 2143 |
| Alw21I | GWGCWC | 4 cut(s) | 548, 742, 916, 1027 |
| Alw26I | GTCTC | 7 cut(s) | 439, 1486, 2069, 2250, 2315, 3386, 3517 |
| AlwI | GGATC | 8 cut(s) | 92, 1546, 1655, 2295, 2360, 2373, 2443, 3438 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 3161, 3556 |
| Ama87I | CYCGRG | 3 cut(s) | 207, 634, 3592 |
| Aor13HI | TCCGGA | 1 cut(s) | 117 |
| Aor51HI | AGCGCT | 2 cut(s) | 349, 1679 |
| AoxI | GGCC | 8 cut(s) | 920, 1223, 1624, 2566, 2858, 2986, 3095, 3588 |
| ApoI | RAATTY | 6 cut(s) | 164, 693, 2097, 2355, 2965, 3064 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3515, 3547 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1248, 2097, 2166 |
| AspLEI | GCGC | 6 cut(s) | 29, 350, 1095, 1440, 1680, 2715 |
| AspS9I | GGNCC | 9 cut(s) | 86, 261, 920, 1868, 2083, 2567, 2620, 3096, 3589 |
| AsuC2I | CCSGG | 2 cut(s) | 155, 3140 |
| AsuHPI | GGTGA | 5 cut(s) | 233, 306, 1556, 2565, 2761 |
| AsuNHI | GCTAGC | 1 cut(s) | 344 |
| AvaI | CYCGRG | 3 cut(s) | 207, 634, 3592 |
| AvaII | GGWCC | 5 cut(s) | 86, 261, 1868, 2083, 2620 |
| AxyI | CCTNAGG | 1 cut(s) | 2250 |
| BaeGI | GKGCMC | 1 cut(s) | 180 |
| BalI | TGGCCA | 1 cut(s) | 2860 |
| BamHI | GGATCC | 1 cut(s) | 2365 |
| BanI | GGYRCC | 2 cut(s) | 595, 2971 |
| BanII | GRGCYC | 4 cut(s) | 150, 548, 1194, 3500 |
| BauI | CACGAG | 2 cut(s) | 1326, 3501 |
| BbsI | GAAGAC | 3 cut(s) | 282, 1549, 3394 |
| Bbv12I | GWGCWC | 4 cut(s) | 548, 742, 916, 1027 |
| BbvCI | CCTCAGC | 2 cut(s) | 516, 1509 |
| BccI | CCATC | 7 cut(s) | 218, 421, 1287, 2569, 2850, 3137, 3648 |
| BceAI | ACGGC | 3 cut(s) | 295, 1067, 1230 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 851, 885, 1286, 1320, 3515, 3549 |
| BciT130I | CCWGG | 6 cut(s) | 219, 1536, 2282, 3162, 3389, 3584 |
| BciVI | GTATCC | 2 cut(s) | 501, 3146 |
| BclI | TGATCA | 1 cut(s) | 2455 |
| BcnI | CCSGG | 2 cut(s) | 155, 3140 |
| BcoDI | GTCTC | 7 cut(s) | 439, 1486, 2069, 2250, 2315, 3386, 3517 |
| BfaI | CTAG | 6 cut(s) | 336, 345, 795, 2108, 2529, 2948 |
| BfmI | CTRYAG | 3 cut(s) | 698, 1716, 2580 |
| BfoI | RGCGCY | 4 cut(s) | 30, 351, 1681, 2716 |
| BfuAI | ACCTGC | 2 cut(s) | 1342, 2203 |
| BfuI | GTATCC | 2 cut(s) | 501, 3146 |
| BglII | AGATCT | 1 cut(s) | 3482 |
| BmcAI | AGTACT | 1 cut(s) | 667 |
| Bme1390I | CCNGG | 8 cut(s) | 155, 219, 1536, 2282, 3140, 3162, 3389, 3584 |
| Bme18I | GGWCC | 5 cut(s) | 86, 261, 1868, 2083, 2620 |
| BmeT110I | CYCGRG | 3 cut(s) | 207, 634, 3592 |
| BmgBI | CACGTC | 2 cut(s) | 1355, 1555 |
| BmgT120I | GGNCC | 9 cut(s) | 86, 261, 920, 1868, 2083, 2567, 2620, 3096, 3589 |
| BmrFI | CCNGG | 8 cut(s) | 155, 219, 1536, 2282, 3140, 3162, 3389, 3584 |
| BmrI | ACTGGG | 2 cut(s) | 2190, 2466 |
| BmtI | GCTAGC | 1 cut(s) | 348 |
| BmuI | ACTGGG | 2 cut(s) | 2190, 2466 |
| BoxI | GACNNNNGTC | 1 cut(s) | 3398 |
| BpiI | GAAGAC | 3 cut(s) | 282, 1549, 3394 |
| BpmI | CTGGAG | 8 cut(s) | 1208, 1249, 1443, 2676, 3183, 3410, 3593, 3605 |
| Bpu10I | CCTNAGC | 3 cut(s) | 516, 762, 1509 |
| BpuEI | CTTGAG | 1 cut(s) | 1623 |
| BpuMI | CCSGG | 2 cut(s) | 155, 3140 |
| Bsa29I | ATCGAT | 2 cut(s) | 425, 3617 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1942 |
| BsaHI | GRCGYC | 1 cut(s) | 158 |
| BsaJI | CCNNGG | 5 cut(s) | 153, 923, 1871, 2130, 2922 |
| BsaWI | WCCGGW | 2 cut(s) | 117, 493 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 112, 142 |
| Bse118I | RCCGGY | 1 cut(s) | 2173 |
| Bse1I | ACTGG | 6 cut(s) | 1204, 1229, 1245, 1426, 2185, 2472 |
| Bse21I | CCTNAGG | 1 cut(s) | 2250 |
| Bse3DI | GCAATG | 4 cut(s) | 987, 2547, 3405, 3568 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1942 |
| BseAI | TCCGGA | 1 cut(s) | 117 |
| BseBI | CCWGG | 6 cut(s) | 219, 1536, 2282, 3162, 3389, 3584 |
| BseCI | ATCGAT | 2 cut(s) | 425, 3617 |
| BseDI | CCNNGG | 5 cut(s) | 153, 923, 1871, 2130, 2922 |
| BseJI | GATNNNNATC | 1 cut(s) | 1942 |
| BseMI | GCAATG | 4 cut(s) | 987, 2547, 3405, 3568 |
| BseMII | CTCAG | 9 cut(s) | 423, 507, 561, 776, 1400, 1500, 2241, 2310, 3185 |
| BseNI | ACTGG | 6 cut(s) | 1204, 1229, 1245, 1426, 2185, 2472 |
| BseRI | GAGGAG | 1 cut(s) | 588 |
| BseSI | GKGCMC | 1 cut(s) | 180 |
| BseYI | CCCAGC | 2 cut(s) | 539, 3556 |
| BsgI | GTGCAG | 4 cut(s) | 662, 777, 1394, 2175 |
| Bsh1236I | CGCG | 1 cut(s) | 385 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2174 |
| BshFI | GGCC | 8 cut(s) | 922, 1225, 1626, 2568, 2860, 2988, 3097, 3590 |
| BshNI | GGYRCC | 2 cut(s) | 595, 2971 |
| BshVI | ATCGAT | 2 cut(s) | 425, 3617 |
| BsiEI | CGRYCG | 1 cut(s) | 2174 |
| BsiHKAI | GWGCWC | 4 cut(s) | 548, 742, 916, 1027 |
| BsiHKCI | CYCGRG | 3 cut(s) | 207, 634, 3592 |
| BsiSI | CCGG | 6 cut(s) | 118, 155, 494, 2174, 3094, 3140 |
| BslFI | GGGAC | 3 cut(s) | 99, 2633, 3453 |
| BsmAI | GTCTC | 7 cut(s) | 439, 1486, 2069, 2250, 2315, 3386, 3517 |
| BsmFI | GGGAC | 3 cut(s) | 99, 2633, 3453 |
| BsmI | GAATGC | 2 cut(s) | 3084, 3339 |
| BsnI | GGCC | 8 cut(s) | 922, 1225, 1626, 2568, 2860, 2988, 3097, 3590 |
| BsoBI | CYCGRG | 3 cut(s) | 207, 634, 3592 |
| Bsp1286I | GDGCHC | 8 cut(s) | 150, 180, 548, 742, 916, 1027, 1194, 3500 |
| Bsp13I | TCCGGA | 1 cut(s) | 117 |
| Bsp19I | CCATGG | 2 cut(s) | 923, 1871 |
| BspACI | CCGC | 7 cut(s) | 317, 341, 383, 593, 1226, 2536, 2576 |
| BspANI | GGCC | 8 cut(s) | 922, 1225, 1626, 2568, 2860, 2988, 3097, 3590 |
| BspCNI | CTCAG | 9 cut(s) | 422, 508, 560, 775, 1399, 1501, 2242, 2311, 3186 |
| BspDI | ATCGAT | 2 cut(s) | 425, 3617 |
| BspEI | TCCGGA | 1 cut(s) | 117 |
| BspFNI | CGCG | 1 cut(s) | 385 |
| BspMAI | CTGCAG | 1 cut(s) | 702 |
| BspMI | ACCTGC | 2 cut(s) | 1342, 2203 |
| BspOI | GCTAGC | 1 cut(s) | 348 |
| BspPI | GGATC | 8 cut(s) | 92, 1546, 1655, 2295, 2360, 2373, 2443, 3438 |
| BspT107I | GGYRCC | 2 cut(s) | 595, 2971 |
| BsrDI | GCAATG | 4 cut(s) | 987, 2547, 3405, 3568 |
| BsrFI | RCCGGY | 1 cut(s) | 2173 |
| BsrI | ACTGG | 6 cut(s) | 1204, 1229, 1245, 1426, 2185, 2472 |
| BssAI | RCCGGY | 1 cut(s) | 2173 |
| BssECI | CCNNGG | 5 cut(s) | 153, 923, 1871, 2130, 2922 |
| BssNAI | GTATAC | 1 cut(s) | 1924 |
| BssNI | GRCGYC | 1 cut(s) | 158 |
| BssSI | CACGAG | 2 cut(s) | 1326, 3501 |
| BssT1I | CCWWGG | 4 cut(s) | 923, 1871, 2130, 2922 |
| Bst1107I | GTATAC | 1 cut(s) | 1924 |
| Bst2BI | CACGAG | 2 cut(s) | 1326, 3501 |
| Bst2UI | CCWGG | 6 cut(s) | 219, 1536, 2282, 3162, 3389, 3584 |
| Bst6I | CTCTTC | 2 cut(s) | 829, 3068 |
| BstACI | GRCGYC | 1 cut(s) | 158 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 947, 1652, 3553 |
| BstC8I | GCNNGC | 9 cut(s) | 346, 471, 1299, 1377, 2208, 2538, 2554, 2629, 3240 |
| BstDSI | CCRYGG | 2 cut(s) | 923, 1871 |
| BstFNI | CGCG | 1 cut(s) | 385 |
| BstH2I | RGCGCY | 4 cut(s) | 30, 351, 1681, 2716 |
| BstHHI | GCGC | 6 cut(s) | 29, 350, 1095, 1440, 1680, 2715 |
| BstMAI | GTCTC | 7 cut(s) | 439, 1486, 2069, 2250, 2315, 3386, 3517 |
| BstMCI | CGRYCG | 1 cut(s) | 2174 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 347, 947, 1266, 1643, 1652, 2195, 3553 |
| BstNI | CCWGG | 6 cut(s) | 219, 1536, 2282, 3162, 3389, 3584 |
| BstNSI | RCATGY | 2 cut(s) | 954, 2044 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 3398 |
| BstSCI | CCNGG | 8 cut(s) | 153, 217, 1534, 2280, 3138, 3160, 3387, 3582 |
| BstSFI | CTRYAG | 3 cut(s) | 698, 1716, 2580 |
| BstSLI | GKGCMC | 1 cut(s) | 180 |
| BstUI | CGCG | 1 cut(s) | 385 |
| BstV2I | GAAGAC | 3 cut(s) | 282, 1549, 3394 |
| BstX2I | RGATCY | 5 cut(s) | 1538, 2300, 2365, 2448, 3482 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1878 |
| BstYI | RGATCY | 5 cut(s) | 1538, 2300, 2365, 2448, 3482 |
| BstZ17I | GTATAC | 1 cut(s) | 1924 |
| Bsu15I | ATCGAT | 2 cut(s) | 425, 3617 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2250 |
| BsuI | GTATCC | 2 cut(s) | 501, 3146 |
| BsuRI | GGCC | 8 cut(s) | 922, 1225, 1626, 2568, 2860, 2988, 3097, 3590 |
| BsuTUI | ATCGAT | 2 cut(s) | 425, 3617 |
| BtgI | CCRYGG | 2 cut(s) | 923, 1871 |
| BtgZI | GCGATG | 1 cut(s) | 922 |
| BtrI | CACGTC | 2 cut(s) | 1355, 1555 |
| BtsI | GCAGTG | 2 cut(s) | 400, 3566 |
| BtsIMutI | CAGTG | 7 cut(s) | 6, 264, 400, 432, 595, 2353, 3566 |
| BveI | ACCTGC | 2 cut(s) | 1342, 2203 |
| Cac8I | GCNNGC | 9 cut(s) | 346, 471, 1299, 1377, 2208, 2538, 2554, 2629, 3240 |
| CaiI | CAGNNNCTG | 2 cut(s) | 3161, 3556 |
| CfoI | GCGC | 6 cut(s) | 29, 350, 1095, 1440, 1680, 2715 |
| Cfr10I | RCCGGY | 1 cut(s) | 2173 |
| Cfr13I | GGNCC | 9 cut(s) | 86, 261, 920, 1868, 2083, 2567, 2620, 3096, 3589 |
| ClaI | ATCGAT | 2 cut(s) | 425, 3617 |
| CseI | GACGC | 4 cut(s) | 147, 947, 2801, 3158 |
| Csp6I | GTAC | 9 cut(s) | 406, 666, 1331, 1383, 1477, 2945, 3363, 3449, 3490 |
| CviQI | GTAC | 9 cut(s) | 406, 666, 1331, 1383, 1477, 2945, 3363, 3449, 3490 |
| DraI | TTTAAA | 1 cut(s) | 3345 |
| DraIII | CACNNNGTG | 1 cut(s) | 1328 |
| EaeI | YGGCCR | 2 cut(s) | 1624, 2858 |
| Eam1104I | CTCTTC | 2 cut(s) | 829, 3068 |
| EarI | CTCTTC | 2 cut(s) | 829, 3068 |
| Ecl136II | GAGCTC | 1 cut(s) | 546 |
| Eco130I | CCWWGG | 4 cut(s) | 923, 1871, 2130, 2922 |
| Eco24I | GRGCYC | 4 cut(s) | 150, 548, 1194, 3500 |
| Eco47I | GGWCC | 5 cut(s) | 86, 261, 1868, 2083, 2620 |
| Eco47III | AGCGCT | 2 cut(s) | 349, 1679 |
| Eco53kI | GAGCTC | 1 cut(s) | 546 |
| Eco57I | CTGAAG | 2 cut(s) | 30, 1083 |
| Eco81I | CCTNAGG | 1 cut(s) | 2250 |
| Eco88I | CYCGRG | 3 cut(s) | 207, 634, 3592 |
| EcoICRI | GAGCTC | 1 cut(s) | 546 |
| EcoO109I | RGGNCCY | 4 cut(s) | 86, 2567, 2620, 3589 |
| EcoRI | GAATTC | 2 cut(s) | 693, 2097 |
| EcoRII | CCWGG | 6 cut(s) | 217, 1534, 2280, 3160, 3387, 3582 |
| EcoT14I | CCWWGG | 4 cut(s) | 923, 1871, 2130, 2922 |
| EcoT22I | ATGCAT | 2 cut(s) | 952, 3602 |
| EcoT38I | GRGCYC | 4 cut(s) | 150, 548, 1194, 3500 |
| ErhI | CCWWGG | 4 cut(s) | 923, 1871, 2130, 2922 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 641, 673 |
| FaqI | GGGAC | 3 cut(s) | 99, 2633, 3453 |
| FauI | CCCGC | 1 cut(s) | 2529 |
| FauNDI | CATATG | 4 cut(s) | 943, 1369, 1456, 1801 |
| FbaI | TGATCA | 1 cut(s) | 2455 |
| FblI | GTMKAC | 2 cut(s) | 1277, 1923 |
| FriOI | GRGCYC | 4 cut(s) | 150, 548, 1194, 3500 |
| FspBI | CTAG | 6 cut(s) | 336, 345, 795, 2108, 2529, 2948 |
| FspI | TGCGCA | 1 cut(s) | 1439 |
| GlaI | GCGC | 6 cut(s) | 28, 349, 1094, 1439, 1679, 2714 |
| GsaI | CCCAGC | 2 cut(s) | 543, 3560 |
| GsuI | CTGGAG | 8 cut(s) | 1208, 1249, 1443, 2676, 3183, 3410, 3593, 3605 |
| HaeII | RGCGCY | 4 cut(s) | 30, 351, 1681, 2716 |
| HaeIII | GGCC | 8 cut(s) | 922, 1225, 1626, 2568, 2860, 2988, 3097, 3590 |
| HapII | CCGG | 6 cut(s) | 118, 155, 494, 2174, 3094, 3140 |
| HgaI | GACGC | 4 cut(s) | 147, 947, 2801, 3158 |
| HhaI | GCGC | 6 cut(s) | 29, 350, 1095, 1440, 1680, 2715 |
| Hin1I | GRCGYC | 1 cut(s) | 158 |
| Hin6I | GCGC | 6 cut(s) | 27, 348, 1093, 1438, 1678, 2713 |
| HinP1I | GCGC | 6 cut(s) | 27, 348, 1093, 1438, 1678, 2713 |
| HpaII | CCGG | 6 cut(s) | 118, 155, 494, 2174, 3094, 3140 |
| HphI | GGTGA | 5 cut(s) | 233, 306, 1556, 2565, 2761 |
| Hpy166II | GTNNAC | 6 cut(s) | 6, 1278, 1331, 1700, 1924, 3149 |
| Hpy8I | GTNNAC | 6 cut(s) | 6, 1278, 1331, 1700, 1924, 3149 |
| HpyCH4IV | ACGT | 3 cut(s) | 1354, 1475, 1554 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 347, 947, 1266, 1643, 1652, 2195, 3553 |
| HpySE526I | ACGT | 3 cut(s) | 1354, 1475, 1554 |
| Hsp92I | GRCGYC | 1 cut(s) | 158 |
| HspAI | GCGC | 6 cut(s) | 27, 348, 1093, 1438, 1678, 2713 |
| KflI | GGGWCCC | 1 cut(s) | 86 |
| Kpn2I | TCCGGA | 1 cut(s) | 117 |
| Ksp22I | TGATCA | 1 cut(s) | 2455 |
| MaeI | CTAG | 6 cut(s) | 336, 345, 795, 2108, 2529, 2948 |
| MaeII | ACGT | 3 cut(s) | 1354, 1475, 1554 |
| MaeIII | GTNAC | 5 cut(s) | 312, 386, 1426, 2831, 3181 |
| MboII | GAAGA | 9 cut(s) | 287, 816, 1088, 1162, 1526, 1549, 2222, 3085, 3394 |
| MflI | RGATCY | 5 cut(s) | 1538, 2300, 2365, 2448, 3482 |
| MhlI | GDGCHC | 8 cut(s) | 150, 180, 548, 742, 916, 1027, 1194, 3500 |
| MlsI | TGGCCA | 1 cut(s) | 2860 |
| MluNI | TGGCCA | 1 cut(s) | 2860 |
| MlyI | GAGTC | 6 cut(s) | 533, 1404, 1638, 1861, 2927, 3388 |
| MmeI | TCCRAC | 1 cut(s) | 2343 |
| Mox20I | TGGCCA | 1 cut(s) | 2860 |
| Mph1103I | ATGCAT | 2 cut(s) | 952, 3602 |
| MroI | TCCGGA | 1 cut(s) | 117 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1248, 2097, 2166 |
| MscI | TGGCCA | 1 cut(s) | 2860 |
| MslI | CAYNNNNRTG | 5 cut(s) | 357, 437, 877, 1526, 1876 |
| Msp20I | TGGCCA | 1 cut(s) | 2860 |
| MspA1I | CMGCKG | 5 cut(s) | 1508, 1667, 1976, 2210, 2631 |
| MspI | CCGG | 6 cut(s) | 118, 155, 494, 2174, 3094, 3140 |
| MspR9I | CCNGG | 8 cut(s) | 155, 219, 1536, 2282, 3140, 3162, 3389, 3584 |
| Mva1269I | GAATGC | 2 cut(s) | 3084, 3339 |
| MvaI | CCWGG | 6 cut(s) | 219, 1536, 2282, 3162, 3389, 3584 |
| MvnI | CGCG | 1 cut(s) | 385 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 347, 947, 1266, 1643, 1652, 2195, 3553 |
| NciI | CCSGG | 2 cut(s) | 155, 3140 |
| NcoI | CCATGG | 2 cut(s) | 923, 1871 |
| NdeI | CATATG | 4 cut(s) | 943, 1369, 1456, 1801 |
| NheI | GCTAGC | 1 cut(s) | 344 |
| NmeAIII | GCCGAG | 1 cut(s) | 1652 |
| NmuCI | GTSAC | 2 cut(s) | 312, 2831 |
| NsbI | TGCGCA | 1 cut(s) | 1439 |
| NsiI | ATGCAT | 2 cut(s) | 952, 3602 |
| NspI | RCATGY | 2 cut(s) | 954, 2044 |
| OliI | CACNNNNGTG | 1 cut(s) | 437 |
| PaeR7I | CTCGAG | 1 cut(s) | 634 |
| PaqCI | CACCTGC | 1 cut(s) | 2203 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 40, 308, 1312 |
| PctI | GAATGC | 2 cut(s) | 3084, 3339 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1248, 2097, 2166 |
| PflFI | GACNNNGTC | 1 cut(s) | 1808 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 752, 929 |
| PfoI | TCCNGGA | 3 cut(s) | 3138, 3160, 3387 |
| PleI | GAGTC | 6 cut(s) | 532, 1404, 1637, 1860, 2926, 3388 |
| PpsI | GAGTC | 6 cut(s) | 532, 1404, 1637, 1860, 2926, 3388 |
| PpuMI | RGGWCCY | 2 cut(s) | 86, 2620 |
| PshAI | GACNNNNGTC | 1 cut(s) | 3398 |
| Psp124BI | GAGCTC | 1 cut(s) | 548 |
| Psp5II | RGGWCCY | 2 cut(s) | 86, 2620 |
| Psp6I | CCWGG | 6 cut(s) | 217, 1534, 2280, 3160, 3387, 3582 |
| PspFI | CCCAGC | 2 cut(s) | 539, 3556 |
| PspGI | CCWGG | 6 cut(s) | 217, 1534, 2280, 3160, 3387, 3582 |
| PspPI | GGNCC | 9 cut(s) | 86, 261, 920, 1868, 2083, 2567, 2620, 3096, 3589 |
| PspPPI | RGGWCCY | 2 cut(s) | 86, 2620 |
| PspXI | VCTCGAGB | 1 cut(s) | 634 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 799, 831, 838, 870 |
| PstI | CTGCAG | 1 cut(s) | 702 |
| PstNI | CAGNNNCTG | 2 cut(s) | 3161, 3556 |
| PsuI | RGATCY | 5 cut(s) | 1538, 2300, 2365, 2448, 3482 |
| PsyI | GACNNNGTC | 1 cut(s) | 1808 |
| PvuII | CAGCTG | 5 cut(s) | 1508, 1667, 1976, 2210, 2631 |
| RsaI | GTAC | 9 cut(s) | 407, 667, 1332, 1384, 1478, 2946, 3364, 3450, 3491 |
| RsaNI | GTAC | 9 cut(s) | 406, 666, 1331, 1383, 1477, 2945, 3363, 3449, 3490 |
| RseI | CAYNNNNRTG | 5 cut(s) | 357, 437, 877, 1526, 1876 |
| SacI | GAGCTC | 1 cut(s) | 548 |
| Sau96I | GGNCC | 9 cut(s) | 86, 261, 920, 1868, 2083, 2567, 2620, 3096, 3589 |
| ScaI | AGTACT | 1 cut(s) | 667 |
| SchI | GAGTC | 6 cut(s) | 533, 1404, 1638, 1861, 2927, 3388 |
| ScrFI | CCNGG | 8 cut(s) | 155, 219, 1536, 2282, 3140, 3162, 3389, 3584 |
| SduI | GDGCHC | 8 cut(s) | 150, 180, 548, 742, 916, 1027, 1194, 3500 |
| SfcI | CTRYAG | 3 cut(s) | 698, 1716, 2580 |
| Sfr274I | CTCGAG | 1 cut(s) | 634 |
| SinI | GGWCC | 5 cut(s) | 86, 261, 1868, 2083, 2620 |
| SlaI | CTCGAG | 1 cut(s) | 634 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 357, 437, 877, 1526, 1876 |
| SmlI | CTYRAG | 2 cut(s) | 634, 1638 |
| SmoI | CTYRAG | 2 cut(s) | 634, 1638 |
| SsiI | CCGC | 7 cut(s) | 317, 341, 383, 593, 1226, 2536, 2576 |
| SspI | AATATT | 3 cut(s) | 1312, 2419, 2647 |
| SspMI | CTAG | 6 cut(s) | 336, 345, 795, 2108, 2529, 2948 |
| SstI | GAGCTC | 1 cut(s) | 548 |
| StyD4I | CCNGG | 8 cut(s) | 153, 217, 1534, 2280, 3138, 3160, 3387, 3582 |
| StyI | CCWWGG | 4 cut(s) | 923, 1871, 2130, 2922 |
| TaiI | ACGT | 3 cut(s) | 1357, 1478, 1557 |
| TaqI | TCGA | 9 cut(s) | 43, 425, 635, 1252, 2170, 2727, 3084, 3433, 3617 |
| TatI | WGTACW | 5 cut(s) | 405, 665, 2944, 3362, 3448 |
| TauI | GCSGC | 2 cut(s) | 344, 1228 |
| TscAI | CASTG | 7 cut(s) | 13, 271, 400, 439, 595, 2353, 3573 |
| TseFI | GTSAC | 2 cut(s) | 312, 2831 |
| Tsp45I | GTSAC | 2 cut(s) | 312, 2831 |
| TspRI | CASTG | 7 cut(s) | 13, 271, 400, 439, 595, 2353, 3573 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1808 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 752, 929 |
| VpaK11BI | GGWCC | 5 cut(s) | 86, 261, 1868, 2083, 2620 |
| XapI | RAATTY | 6 cut(s) | 164, 693, 2097, 2355, 2965, 3064 |
| XceI | RCATGY | 2 cut(s) | 954, 2044 |
| XhoI | CTCGAG | 1 cut(s) | 634 |
| XmiI | GTMKAC | 2 cut(s) | 1277, 1923 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1248, 2097, 2166 |
| XspI | CTAG | 6 cut(s) | 336, 345, 795, 2108, 2529, 2948 |
| ZrmI | AGTACT | 1 cut(s) | 667 |
| Zsp2I | ATGCAT | 2 cut(s) | 952, 3602 |