FvH4_6g05151

Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006264
Physical Location & Seq
Reverse (-)
2886596 .. 2888506
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05151.t1

Sequence Viewer

Length: 1911 bp
ATGGCCGAAGAACCTACGACTCCTCCTCCTGATCTCAATAAACGCGAGCCAGAGCAGGCCGGGAGTACTTTCTCTCCTTCTAGCAGTGTCGACTCCAACATCCCGAAAGGAGAGCTCCAGTTCACTCCTTCTTCCATTACTTCTAAGCTGCGTGGGCCTGACTTTAGTTCGGATGGTGTTACTAACGATGAAGACCCTACGACTCCTCCTCCTAGTCTTAGTGAACAAGATTCAGAGCAGGCCGGGAGTACTATCTCTGCTCGTGGTAGTGTCAGCTCCAACATTGTCATCCAGCAGAAAGCAAAGACTGCTACTCCTTCCACACAGAACAGTGTTGTCGGATTTAATAACACTGATGATGAATATGAGCAGCACAGTGTTAGTAATGACGACTTAAGTACCAAGATAGCTAACCTTAACCTCACGGATGGTGTTACTGGCGTCACAAAGCAGGCACAGAAGGTGAACTCAATTGACCTGGAGGAATCAGAATGTACTGTATATACTGATGGCTACTCACAAGCGTTAGCGGCAGAGAATGAAAGTAGTTGTTCGCGCGTGGCAAGCATGGGCATATTACTCAAGAAGGAGTTCGATGACAATGGAAATTTTCATTTTGTGAGACAATCTTTTCCCCTAGGTCCAAGCCAAGAGGCTATACGAAAAGAAGAAGAGGAGCTTCGTAAACTCAAGGAAGATAAAGGTATTGCAAATGACGATTCTATTGTGAAGTCTCTTGAAAAGTTCAAGGAAGAGTATCTACGGACCAAACCCACCTACCCTAGTTTAAAACAATTAAGAAGGAACAATTTGCGAGGGTACAAGGATCTGTGGCAAGTCAAGGACATGCCTAAGAAGAGTGTTTGTAGAAAGATTGAAAGGCTATATCATATTTTGAGGAAACAGAAAAACCGCGGAGATGGGTACCGTTTTGAATTGCGACTGGAAAAAGAAATTGTCTACGTTCCAGAGCTGGAATGCTATATGTTAAATGGCGAAAGGAACTATCGCTGCGAATATGACAAAGTAAAAACCAGAAGCGTGTTCGTGAGGATGGATGTTCTTCTTTGCCTGATATACAATCTTTTAAACAAAGAGATGACTGTCAACATTCGTTGGATTCCGTATACATGGAGCGAGATATTTAACGATACTGACGATGTTGAATATGTGTTGGACCGCATATGTAAACATATAGGTTGCAGCCGGGACAACATAAATATCTTTGCGTCAGCTAAAGGGAGGATTGCTGGTGATATTAGTTTTAAAGCCACCACTGAAACTTATTGTCAAGGAGGAAGTACTTATTGTCAAGGCGGGGCTGATATAGAGCCTCGTACCGTTGCCGAGGAACTTGAGTTTACTACTTTAAGCAAGCAAGAGCTCTTGCTAATATTGGTTGTCGAAAAGGAGACAATCTTTCAGAGACTTTTGGAGGACAAAATTCATAAGAGATTCCGTTGCATCATAATCACCGGATGCGGAGTGCCAACTGTTGCAACTCGGACTCTATTAAACAAGGTTCAGGCATATTACGATCTCCCAGCAGTATCCCTTGTAGATCACAATGCAGGTGGCTTCAATATTTACTTGAATTACCTGTGCGGCAGCAAGAATATGTCGTACAACTCTTTAAACTTACGCACACCGGGTATTAAGTGGCTGGGTTTGAGTCCTTCAGACCTCATAGAACATAATATTGAAGCAATTGAGAATGAGAATGTGCAAGGACTTGAAACGATCATGAATAATGATTTTGTCAAGAAATCTCAGGAATTGGTCAAGGAGGTTGGGAAAATAAAATATAAAGTCGACATTGAGGGTATGATGAGTAAGAACGCCAAATATTTCACTGAGATATTTGTGCCGCTAAAGTTCAAAAGGTTGGGCGATGGTCGGACATATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04113 map04113
Pfam Domains
Protein Families
TP6A_N

Protein Analysis

637

Amino Acids

72.0

Weight (kDa)

5.72

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOP6A-Spo11_Toprim PF21180 465 - 625 1.1e-40 Topoisomerase 6 subunit A/Spo11, Toprim domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1562
Acc36I ACCTGC 1 cut(s) 1562
Acc65I GGTACC 1 cut(s) 924
AccB1I GGYRCC 1 cut(s) 924
AccI GTMKAC 4 cut(s) 90, 960, 1127, 1812
AccII CGCG 4 cut(s) 45, 556, 558, 915
AciI CCGC 8 cut(s) 530, 913, 915, 1180, 1317, 1482, 1605, 1868
AclWI GGATC 1 cut(s) 834
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 607, 1443
AcuI CTGAAG 1 cut(s) 1662
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 9 cut(s) 67, 250, 400, 496, 821, 926, 1303, 1339, 1625
AflII CTTAAG 1 cut(s) 394
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 8 cut(s) 115, 148, 276, 410, 679, 973, 1235, 1384
AluI AGCT 8 cut(s) 115, 148, 276, 410, 679, 973, 1235, 1384
Alw21I GWGCWC 2 cut(s) 117, 1386
Alw26I GTCTC 4 cut(s) 616, 738, 1406, 1420
AlwI GGATC 1 cut(s) 834
AoxI GGCC 4 cut(s) 3, 57, 155, 240
ApeKI GCWGC 5 cut(s) 148, 370, 1011, 1203, 1608
ApoI RAATTY 2 cut(s) 607, 1443
Asp700I GAANNNNTTC 1 cut(s) 590
Asp718I GGTACC 1 cut(s) 924
AspA2I CCTAGG 1 cut(s) 637
AspLEI GCGC 1 cut(s) 558
AspS9I GGNCC 4 cut(s) 155, 641, 765, 1177
AsuC2I CCSGG 4 cut(s) 61, 244, 1208, 1650
AsuHPI GGTGA 3 cut(s) 475, 1265, 1465
AvaII GGWCC 3 cut(s) 641, 765, 1177
AvrII CCTAGG 1 cut(s) 637
BaeI ACNNNNGTAYC 2 cut(s) 382, 415
BanI GGYRCC 1 cut(s) 924
BanII GRGCYC 2 cut(s) 117, 1386
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
BauI CACGAG 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 117, 1386
BbvI GCAGC 5 cut(s) 135, 382, 998, 1215, 1620
BccI CCATC 6 cut(s) 167, 422, 503, 914, 1048, 1886
BciT130I CCWGG 1 cut(s) 479
BciVI GTATCC 1 cut(s) 1561
BcnI CCSGG 4 cut(s) 61, 244, 1208, 1650
BcoDI GTCTC 4 cut(s) 616, 738, 1406, 1420
BfaI CTAG 4 cut(s) 81, 213, 638, 783
BfrI CTTAAG 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 1562
BfuI GTATCC 1 cut(s) 1561
BisI GCNGC 8 cut(s) 149, 371, 531, 1012, 1204, 1606, 1609, 1868
BlnI CCTAGG 1 cut(s) 637
BlsI GCNGC 8 cut(s) 150, 372, 532, 1013, 1205, 1607, 1610, 1869
BmcAI AGTACT 3 cut(s) 67, 250, 1303
Bme1390I CCNGG 5 cut(s) 61, 244, 479, 1208, 1650
Bme18I GGWCC 3 cut(s) 641, 765, 1177
BmgT120I GGNCC 4 cut(s) 155, 641, 765, 1177
BmiI GGNNCC 1 cut(s) 926
BmrFI CCNGG 5 cut(s) 61, 244, 479, 1208, 1650
BmsI GCATC 2 cut(s) 1469, 1473
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 2 cut(s) 101, 500
BpuEI CTTGAG 3 cut(s) 566, 674, 1376
BpuMI CCSGG 4 cut(s) 61, 244, 1208, 1650
BsaHI GRCGYC 1 cut(s) 441
BsaJI CCNNGG 3 cut(s) 637, 913, 1347
BsaWI WCCGGW 1 cut(s) 1475
Bse1I ACTGG 3 cut(s) 118, 442, 948
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 3 cut(s) 637, 913, 1347
BseGI GGATG 7 cut(s) 99, 178, 288, 433, 1059, 1063, 1484
BseMII CTCAG 2 cut(s) 1784, 1845
BseNI ACTGG 3 cut(s) 118, 442, 948
BseRI GAGGAG 5 cut(s) 12, 15, 195, 198, 689
BseXI GCAGC 5 cut(s) 135, 382, 998, 1215, 1620
BseYI CCCAGC 2 cut(s) 1543, 1663
Bsh1236I CGCG 4 cut(s) 45, 556, 558, 915
BshFI GGCC 4 cut(s) 5, 59, 157, 242
BshNI GGYRCC 1 cut(s) 924
BsiHKAI GWGCWC 2 cut(s) 117, 1386
BsiSI CCGG 5 cut(s) 60, 243, 1207, 1476, 1649
BslFI GGGAC 1 cut(s) 1223
BsmAI GTCTC 4 cut(s) 616, 738, 1406, 1420
BsmFI GGGAC 1 cut(s) 1223
BsmI GAATGC 1 cut(s) 983
BsnI GGCC 4 cut(s) 5, 59, 157, 242
Bsp1286I GDGCHC 2 cut(s) 117, 1386
Bsp143I GATC 5 cut(s) 31, 826, 1537, 1561, 1740
BspACI CCGC 8 cut(s) 530, 913, 915, 1180, 1317, 1482, 1605, 1868
BspANI GGCC 4 cut(s) 5, 59, 157, 242
BspCNI CTCAG 2 cut(s) 1783, 1846
BspFNI CGCG 4 cut(s) 45, 556, 558, 915
BspHI TCATGA 1 cut(s) 1743
BspLI GGNNCC 1 cut(s) 926
BspMI ACCTGC 1 cut(s) 1562
BspPI GGATC 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 924
BspTI CTTAAG 1 cut(s) 394
BsrI ACTGG 3 cut(s) 118, 442, 948
BssECI CCNNGG 3 cut(s) 637, 913, 1347
BssMI GATC 5 cut(s) 31, 826, 1537, 1561, 1740
BssNAI GTATAC 1 cut(s) 1128
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 637
Bst1107I GTATAC 1 cut(s) 1128
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 7 cut(s) 332, 377, 499, 929, 1105, 1342, 1495
Bst6I CTCTTC 3 cut(s) 666, 747, 851
BstACI GRCGYC 1 cut(s) 441
BstAFI CTTAAG 1 cut(s) 394
BstAPI GCANNNNNTGC 1 cut(s) 308
BstC8I GCNNGC 6 cut(s) 47, 57, 240, 453, 565, 1376
BstDEI CTNAG 5 cut(s) 144, 218, 852, 1770, 1854
BstDSI CCRYGG 1 cut(s) 913
BstF5I GGATG 7 cut(s) 99, 178, 288, 433, 1059, 1063, 1484
BstFNI CGCG 4 cut(s) 45, 556, 558, 915
BstHHI GCGC 1 cut(s) 558
BstKTI GATC 5 cut(s) 34, 829, 1540, 1564, 1743
BstMAI GTCTC 4 cut(s) 616, 738, 1406, 1420
BstMBI GATC 5 cut(s) 31, 826, 1537, 1561, 1740
BstMWI GCNNNNNNNGC 4 cut(s) 154, 308, 530, 564
BstNI CCWGG 1 cut(s) 479
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 5 cut(s) 59, 242, 477, 1206, 1648
BstUI CGCG 4 cut(s) 45, 556, 558, 915
BstV1I GCAGC 5 cut(s) 135, 382, 998, 1215, 1620
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BstZ17I GTATAC 1 cut(s) 1128
BsuI GTATCC 1 cut(s) 1561
BsuRI GGCC 4 cut(s) 5, 59, 157, 242
BtgI CCRYGG 1 cut(s) 913
BtgZI GCGATG 1 cut(s) 1905
BtsCI GGATG 7 cut(s) 99, 178, 288, 433, 1059, 1063, 1484
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 6 cut(s) 91, 337, 351, 382, 1275, 1851
BveI ACCTGC 1 cut(s) 1562
Cac8I GCNNGC 6 cut(s) 47, 57, 240, 453, 565, 1376
CciI TCATGA 1 cut(s) 1743
CfoI GCGC 1 cut(s) 558
Cfr13I GGNCC 4 cut(s) 155, 641, 765, 1177
Cfr42I CCGCGG 1 cut(s) 916
CseI GACGC 2 cut(s) 430, 1218
Csp6I GTAC 9 cut(s) 66, 249, 399, 495, 820, 925, 1302, 1338, 1624
CspCI CAANNNNNGTGG 2 cut(s) 1555, 1590
CviAII CATG 4 cut(s) 568, 847, 1131, 1744
CviQI GTAC 9 cut(s) 66, 249, 399, 495, 820, 925, 1302, 1338, 1624
DdeI CTNAG 5 cut(s) 144, 218, 852, 1770, 1854
DpnI GATC 5 cut(s) 33, 828, 1539, 1563, 1742
DpnII GATC 5 cut(s) 31, 826, 1537, 1561, 1740
DraI TTTAAA 4 cut(s) 789, 1089, 1267, 1635
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 666, 747, 851
EarI CTCTTC 3 cut(s) 666, 747, 851
Ecl136II GAGCTC 2 cut(s) 115, 1384
Eco130I CCWWGG 1 cut(s) 637
Eco24I GRGCYC 2 cut(s) 117, 1386
Eco47I GGWCC 3 cut(s) 641, 765, 1177
Eco53kI GAGCTC 2 cut(s) 115, 1384
Eco57I CTGAAG 1 cut(s) 1662
EcoICRI GAGCTC 2 cut(s) 115, 1384
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 637
EcoT38I GRGCYC 2 cut(s) 117, 1386
ErhI CCWWGG 1 cut(s) 637
FaeI CATG 4 cut(s) 571, 850, 1134, 1747
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FaqI GGGAC 1 cut(s) 1223
FatI CATG 4 cut(s) 567, 846, 1130, 1743
FauI CCCGC 1 cut(s) 1310
FauNDI CATATG 1 cut(s) 1184
FblI GTMKAC 4 cut(s) 90, 960, 1127, 1812
Fnu4HI GCNGC 8 cut(s) 149, 371, 531, 1012, 1204, 1606, 1609, 1868
FokI GGATG 7 cut(s) 86, 185, 275, 440, 1066, 1070, 1491
FriOI GRGCYC 2 cut(s) 117, 1386
Fsp4HI GCNGC 8 cut(s) 149, 371, 531, 1012, 1204, 1606, 1609, 1868
FspBI CTAG 4 cut(s) 81, 213, 638, 783
GlaI GCGC 1 cut(s) 557
GluI GCNGC 8 cut(s) 149, 371, 531, 1012, 1204, 1606, 1609, 1868
GsaI CCCAGC 2 cut(s) 1547, 1667
GsuI CTGGAG 2 cut(s) 101, 500
HaeIII GGCC 4 cut(s) 5, 59, 157, 242
HapII CCGG 5 cut(s) 60, 243, 1207, 1476, 1649
HgaI GACGC 2 cut(s) 430, 1218
HhaI GCGC 1 cut(s) 558
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 4 cut(s) 571, 850, 1134, 1747
Hin6I GCGC 1 cut(s) 556
HinP1I GCGC 1 cut(s) 556
HincII GTYRAC 3 cut(s) 91, 1108, 1813
HindII GTYRAC 3 cut(s) 91, 1108, 1813
HpaII CCGG 5 cut(s) 60, 243, 1207, 1476, 1649
HphI GGTGA 3 cut(s) 475, 1265, 1465
Hpy188I TCNGA 8 cut(s) 172, 235, 341, 490, 1425, 1506, 1681, 1899
Hpy188III TCNNGA 9 cut(s) 29, 103, 583, 737, 968, 1048, 1744, 1762, 1772
HpyAV CCTTC 7 cut(s) 87, 138, 327, 454, 580, 795, 1686
HpyCH4III ACNGT 7 cut(s) 332, 377, 499, 929, 1105, 1342, 1495
HpyCH4IV ACGT 1 cut(s) 963
HpyCH4V TGCA 6 cut(s) 710, 1203, 1464, 1499, 1571, 1726
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 308, 530, 564
HpyF3I CTNAG 5 cut(s) 144, 218, 852, 1770, 1854
HpySE526I ACGT 1 cut(s) 963
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 4 cut(s) 571, 850, 1134, 1747
HspAI GCGC 1 cut(s) 556
KpnI GGTACC 1 cut(s) 928
KspI CCGCGG 1 cut(s) 916
Kzo9I GATC 5 cut(s) 31, 826, 1537, 1561, 1740
LmnI GCTCC 4 cut(s) 120, 281, 676, 1134
Lsp1109I GCAGC 5 cut(s) 135, 382, 998, 1215, 1620
LweI GCATC 2 cut(s) 1469, 1473
MaeI CTAG 4 cut(s) 81, 213, 638, 783
MaeII ACGT 1 cut(s) 963
MaeIII GTNAC 3 cut(s) 178, 433, 442
MalI GATC 5 cut(s) 33, 828, 1539, 1563, 1742
MboI GATC 5 cut(s) 31, 826, 1537, 1561, 1740
MboII GAAGA 9 cut(s) 20, 123, 203, 680, 683, 707, 764, 868, 1055
MfeI CAATTG 2 cut(s) 471, 1707
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 2 cut(s) 117, 1386
MlyI GAGTC 5 cut(s) 13, 86, 196, 1501, 1681
MmeI TCCRAC 6 cut(s) 120, 303, 319, 1097, 1155, 1877
MroXI GAANNNNTTC 1 cut(s) 590
MslI CAYNNNNRTG 1 cut(s) 1906
MspA1I CMGCKG 1 cut(s) 915
MspCI CTTAAG 1 cut(s) 394
MspI CCGG 5 cut(s) 60, 243, 1207, 1476, 1649
MspR9I CCNGG 5 cut(s) 61, 244, 479, 1208, 1650
MunI CAATTG 2 cut(s) 471, 1707
Mva1269I GAATGC 1 cut(s) 983
MvaI CCWGG 1 cut(s) 479
MvnI CGCG 4 cut(s) 45, 556, 558, 915
MwoI GCNNNNNNNGC 4 cut(s) 154, 308, 530, 564
NciI CCSGG 4 cut(s) 61, 244, 1208, 1650
NdeI CATATG 1 cut(s) 1184
NdeII GATC 5 cut(s) 31, 826, 1537, 1561, 1740
NlaIII CATG 4 cut(s) 571, 850, 1134, 1747
NlaIV GGNNCC 1 cut(s) 926
NmeAIII GCCGAG 1 cut(s) 1372
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 1 cut(s) 850
PagI TCATGA 1 cut(s) 1743
PaqCI CACCTGC 1 cut(s) 1562
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PctI GAATGC 1 cut(s) 983
PdmI GAANNNNTTC 1 cut(s) 590
PfeI GAWTC 5 cut(s) 230, 485, 719, 1120, 1455
PkrI GCNGC 8 cut(s) 150, 372, 532, 1013, 1205, 1607, 1610, 1869
PleI GAGTC 5 cut(s) 13, 86, 196, 1501, 1680
PpsI GAGTC 5 cut(s) 13, 86, 196, 1501, 1680
Psp124BI GAGCTC 2 cut(s) 117, 1386
Psp6I CCWGG 1 cut(s) 477
PspFI CCCAGC 2 cut(s) 1543, 1663
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 1 cut(s) 926
PspPI GGNCC 4 cut(s) 155, 641, 765, 1177
PsuI RGATCY 1 cut(s) 826
RsaI GTAC 9 cut(s) 67, 250, 400, 496, 821, 926, 1303, 1339, 1625
RsaNI GTAC 9 cut(s) 66, 249, 399, 495, 820, 925, 1302, 1338, 1624
RseI CAYNNNNRTG 1 cut(s) 1906
SacI GAGCTC 2 cut(s) 117, 1386
SacII CCGCGG 1 cut(s) 916
SalI GTCGAC 2 cut(s) 89, 1811
SatI GCNGC 8 cut(s) 149, 371, 531, 1012, 1204, 1606, 1609, 1868
Sau3AI GATC 5 cut(s) 31, 826, 1537, 1561, 1740
Sau96I GGNCC 4 cut(s) 155, 641, 765, 1177
ScaI AGTACT 3 cut(s) 67, 250, 1303
SchI GAGTC 5 cut(s) 13, 86, 196, 1501, 1681
ScrFI CCNGG 5 cut(s) 61, 244, 479, 1208, 1650
SduI GDGCHC 2 cut(s) 117, 1386
SfaNI GCATC 2 cut(s) 1469, 1473
Sfr303I CCGCGG 1 cut(s) 916
SgrBI CCGCGG 1 cut(s) 916
SinI GGWCC 3 cut(s) 641, 765, 1177
SmiMI CAYNNNNRTG 1 cut(s) 1906
SmlI CTYRAG 4 cut(s) 394, 581, 689, 1355
SmoI CTYRAG 4 cut(s) 394, 581, 689, 1355
SsiI CCGC 8 cut(s) 530, 913, 915, 1180, 1317, 1482, 1605, 1868
SspI AATATT 4 cut(s) 1395, 1585, 1699, 1847
SspMI CTAG 4 cut(s) 81, 213, 638, 783
SstI GAGCTC 2 cut(s) 117, 1386
StyD4I CCNGG 5 cut(s) 59, 242, 477, 1206, 1648
StyI CCWWGG 1 cut(s) 637
TaaI ACNGT 7 cut(s) 332, 377, 499, 929, 1105, 1342, 1495
TaiI ACGT 1 cut(s) 966
TaqI TCGA 4 cut(s) 90, 594, 1404, 1812
TatI WGTACW 4 cut(s) 65, 248, 494, 1301
TauI GCSGC 3 cut(s) 533, 1608, 1870
TfiI GAWTC 5 cut(s) 230, 485, 719, 1120, 1455
TscAI CASTG 6 cut(s) 91, 337, 358, 382, 1282, 1858
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 5 cut(s) 148, 370, 1011, 1203, 1608
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 6 cut(s) 204, 375, 555, 602, 1436, 1760
TspGWI ACGGA 4 cut(s) 440, 778, 1113, 1448
TspRI CASTG 6 cut(s) 91, 337, 358, 382, 1282, 1858
Vha464I CTTAAG 1 cut(s) 394
VpaK11BI GGWCC 3 cut(s) 641, 765, 1177
XapI RAATTY 2 cut(s) 607, 1443
XceI RCATGY 1 cut(s) 850
XmaJI CCTAGG 1 cut(s) 637
XmiI GTMKAC 4 cut(s) 90, 960, 1127, 1812
XmnI GAANNNNTTC 1 cut(s) 590
XspI CTAG 4 cut(s) 81, 213, 638, 783
ZrmI AGTACT 3 cut(s) 67, 250, 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.