FvH4_6g09130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014347
Physical Location & Seq
Reverse (-)
5410555 .. 5411058
504 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09130.t1

Sequence Viewer

Length: 396 bp
ATGAATGACTACGAGTTCATAATCCGTAACACCATCAAGTCACTTGTAGCCTTGGTCCTCTTCATGGCGGTATTAACCATAAGTAGTACTACTACCACTTCTGCAGTATCTCATGATGATGTTCTTGAGTTTATTCCAATGAAGAATTCGGCGGAGTTTCGAGTAATGGACGTGGTAAGAGCTCACGACGAAGTACGAAGACGGAGGCTGGAGCCCTACCAGCTCTGCTTGGTTTGCAAGTGCTGCGCCGGCACGACATGCTCCACCATGCCTTGCTGTTTCGGCATAGACTGCCAGCTTCCCGGGAAGCCCTTCGGGGTTTGCGCCTTTGTTCCAAAGATCTGCAACTGTGTCAACTGTACAAGTAGTACTAATAATGCTAGTAGAACTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.48

Weight (kDa)

7.98

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7866 PF25268 71 - 121 2.3e-19 Domain of unknown function (DUF7866)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014347)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01015 AT5G01015
fragaria_vesca FvH4_6g09130
malus_domestica MD04G1180500.v1.1 MD12G1195900.v1.1
prunus_persica Prupe.6G300400_v2.0.a1
pyrus_communis pycom04g15960 pycom12g18210
rosa_chinensis RchiOBHm_Chr3g0459341
rosa_laevigata RLG00000025089
rosa_roxburghii Rroxscaffold_6G00420250
rosa_rugosa Rorug03G0035500
rosa_samantha Rh3AG093600 Rh3BG096500 Rh3CG097700 Rh3DG097100
rosa_wichuraiana Rw3G008050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 68, 152
AcsI RAATTY 1 cut(s) 145
AfaI GTAC 4 cut(s) 88, 195, 361, 370
AfiI CCNNNNNNNGG 1 cut(s) 64
AjiI CACGTC 1 cut(s) 172
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AluBI AGCT 3 cut(s) 182, 223, 298
AluI AGCT 3 cut(s) 182, 223, 298
Alw21I GWGCWC 1 cut(s) 184
Ama87I CYCGRG 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 145
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 2 cut(s) 248, 326
AspS9I GGNCC 1 cut(s) 55
AsuC2I CCSGG 2 cut(s) 303, 304
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 55
BanII GRGCYC 2 cut(s) 184, 216
BbsI GAAGAC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 230
BccI CCATC 1 cut(s) 41
BcnI CCSGG 2 cut(s) 303, 304
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 102
BglII AGATCT 1 cut(s) 339
BisI GCNGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 245
BmcAI AGTACT 2 cut(s) 88, 370
Bme1390I CCNGG 2 cut(s) 303, 304
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 2 cut(s) 303, 304
BpiI GAAGAC 1 cut(s) 205
BpmI CTGGAG 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 2 cut(s) 303, 304
BsaJI CCNNGG 2 cut(s) 51, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 248
BseDI CCNNGG 2 cut(s) 51, 302
BseLI CCNNNNNNNGG 1 cut(s) 64
BseXI GCAGC 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 184
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 2 cut(s) 249, 303
BslI CCNNNNNNNGG 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 184, 216
Bsp1407I TGTACA 1 cut(s) 359
Bsp143I GATC 1 cut(s) 339
BspACI CCGC 2 cut(s) 68, 152
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 51, 302
BssMI GATC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 2 cut(s) 350, 359
Bst6I CTCTTC 1 cut(s) 65
BstAPI GCANNNNNTGC 3 cut(s) 243, 258, 291
BstAUI TGTACA 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 250, 296
BstHHI GCGC 2 cut(s) 248, 326
BstKTI GATC 1 cut(s) 342
BstMBI GATC 1 cut(s) 339
BstMWI GCNNNNNNNGC 7 cut(s) 220, 234, 243, 249, 258, 282, 291
BstNSI RCATGY 1 cut(s) 261
BstSCI CCNGG 2 cut(s) 301, 302
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 230
BstV2I GAAGAC 1 cut(s) 205
BstX2I RGATCY 1 cut(s) 339
BstYI RGATCY 1 cut(s) 339
BtrI CACGTC 1 cut(s) 172
Cac8I GCNNGC 2 cut(s) 250, 296
CciI TCATGA 1 cut(s) 112
CfoI GCGC 2 cut(s) 248, 326
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 55
Cfr9I CCCGGG 1 cut(s) 302
Csp6I GTAC 4 cut(s) 87, 194, 360, 369
CviAII CATG 4 cut(s) 64, 113, 258, 268
CviJI RGCY 7 cut(s) 50, 182, 208, 214, 223, 298, 310
CviKI_1 RGCY 7 cut(s) 50, 182, 208, 214, 223, 298, 310
CviQI GTAC 4 cut(s) 87, 194, 360, 369
DpnI GATC 1 cut(s) 341
DpnII GATC 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 184, 216
Eco47I GGWCC 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 184, 216
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 4 cut(s) 67, 116, 261, 271
FaiI YATR 7 cut(s) 20, 65, 80, 114, 259, 269, 287
FatI CATG 4 cut(s) 63, 112, 257, 267
Fnu4HI GCNGC 1 cut(s) 244
FriOI GRGCYC 2 cut(s) 184, 216
Fsp4HI GCNGC 1 cut(s) 244
FspBI CTAG 1 cut(s) 381
GlaI GCGC 2 cut(s) 247, 325
GluI GCNGC 1 cut(s) 244
GsuI CTGGAG 1 cut(s) 230
HapII CCGG 2 cut(s) 249, 303
HhaI GCGC 2 cut(s) 248, 326
Hin1II CATG 4 cut(s) 67, 116, 261, 271
Hin6I GCGC 2 cut(s) 246, 324
HinP1I GCGC 2 cut(s) 246, 324
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HpaII CCGG 2 cut(s) 249, 303
Hpy166II GTNNAC 1 cut(s) 355
Hpy188III TCNNGA 3 cut(s) 113, 125, 185
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 2 cut(s) 350, 359
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 104, 237, 345
HpyF10VI GCNNNNNNNGC 7 cut(s) 220, 234, 243, 249, 258, 282, 291
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 4 cut(s) 67, 116, 261, 271
HspAI GCGC 2 cut(s) 246, 324
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 211, 266
LpnPI CCDG 5 cut(s) 194, 233, 262, 308, 316
Lsp1109I GCAGC 1 cut(s) 230
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 26, 39
MalI GATC 1 cut(s) 341
MboI GATC 1 cut(s) 339
MboII GAAGA 3 cut(s) 52, 154, 210
MflI RGATCY 1 cut(s) 339
MhlI GDGCHC 2 cut(s) 184, 216
MluCI AATT 1 cut(s) 145
MnlI CCTC 2 cut(s) 68, 198
MroNI GCCGGC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 2 cut(s) 249, 303
MspR9I CCNGG 2 cut(s) 303, 304
MwoI GCNNNNNNNGC 7 cut(s) 220, 234, 243, 249, 258, 282, 291
NaeI GCCGGC 1 cut(s) 250
NciI CCSGG 2 cut(s) 303, 304
NdeII GATC 1 cut(s) 339
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 4 cut(s) 67, 116, 261, 271
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 39
NspI RCATGY 1 cut(s) 261
PagI TCATGA 1 cut(s) 112
PdiI GCCGGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 311
PkrI GCNGC 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 1 cut(s) 55
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 339
RsaI GTAC 4 cut(s) 88, 195, 361, 370
RsaNI GTAC 4 cut(s) 87, 194, 360, 369
RseI CAYNNNNRTG 1 cut(s) 117
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 1 cut(s) 244
Sau3AI GATC 1 cut(s) 339
Sau96I GGNCC 1 cut(s) 55
ScaI AGTACT 2 cut(s) 88, 370
ScrFI CCNGG 2 cut(s) 303, 304
SduI GDGCHC 2 cut(s) 184, 216
SetI ASST 4 cut(s) 174, 184, 225, 300
SfcI CTRYAG 1 cut(s) 102
SinI GGWCC 1 cut(s) 55
SmaI CCCGGG 1 cut(s) 304
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 1 cut(s) 145
SsiI CCGC 2 cut(s) 68, 152
SspMI CTAG 1 cut(s) 381
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 301, 302
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 350, 359
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 160
TasI AATT 1 cut(s) 145
TatI WGTACW 3 cut(s) 86, 359, 368
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TseFI GTSAC 1 cut(s) 39
TseI GCWGC 1 cut(s) 243
Tsp45I GTSAC 1 cut(s) 39
TspDTI ATGAA 4 cut(s) 7, 17, 52, 155
TspGWI ACGGA 2 cut(s) 14, 217
TspMI CCCGGG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 145
XceI RCATGY 1 cut(s) 261
XmaI CCCGGG 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 381
ZrmI AGTACT 2 cut(s) 88, 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.